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https://www.readbyqxmd.com/read/27913833/tight-repulsion-linkage-between-sr36-and-sr39-was-revealed-by-genetic-cytogenetic-and-molecular-analyses
#1
Bosco Chemayek, Urmil K Bansal, Naeela Qureshi, Peng Zhang, William W Wagoire, Harbans S Bariana
The shortening of Aegilops speltoides segment did not facilitate recombination between stem rust resistance genes Sr36 and Sr39 . Robustness of marker rwgs28 for marker-assisted selection of Sr39 was demonstrated. Stem rust resistance genes Sr39 and Sr36 were transferred from Aegilops speltoides and Triticum timopheevii, respectively, to chromosome 2B of wheat. Genetic stocks RL6082 and RWG1 carrying Sr39 on a large and a shortened Ae. speltoides segments, respectively, and the Sr36-carrying Australian wheat cultivar Cook were used in this study...
December 2, 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/27909815/homoeologous-recombination-in-the-presence-of-ph1-gene-in-wheat
#2
Dal-Hoe Koo, Wenxuan Liu, Bernd Friebe, Bikram S Gill
A crossover (CO) and its cytological signature, the chiasma, are major features of eukaryotic meiosis. The formation of at least one CO/chiasma between homologous chromosome pairs is essential for accurate chromosome segregation at the first meiotic division and genetic recombination. Polyploid organisms with multiple sets of homoeologous chromosomes have evolved additional mechanisms for the regulation of CO/chiasma. In hexaploid wheat (2n = 6× = 42), this is accomplished by pairing homoeologous (Ph) genes, with Ph1 having the strongest effect on suppressing homoeologous recombination and homoeologous COs...
December 1, 2016: Chromosoma
https://www.readbyqxmd.com/read/27900504/a-large-scale-chromosome-specific-snp-discovery-guideline
#3
Bala Ani Akpinar, Stuart Lucas, Hikmet Budak
Single-nucleotide polymorphisms (SNPs) are the most prevalent type of variation in genomes that are increasingly being used as molecular markers in diversity analyses, mapping and cloning of genes, and germplasm characterization. However, only a few studies reported large-scale SNP discovery in Aegilops tauschii, restricting their potential use as markers for the low-polymorphic D genome. Here, we report 68,592 SNPs found on the gene-related sequences of the 5D chromosome of Ae. tauschii genotype MvGB589 using genomic and transcriptomic sequences from seven Ae...
November 29, 2016: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/27887035/impact-of-transgene-genome-location-on-gene-migration-from-herbicide-resistant-wheat-triticum-aestivum-l-to-jointed-goatgrass-aegilops-cylindrica-host
#4
M Rehman, J L Hansen, C A Mallory-Smith, R S Zemetra
BACKGROUND: Wheat (Triticum aestivum) (ABD) and jointed goatgrass (Aegilops cylindrica) (CD) can cross and produce hybrids that can backcross to either parent. Such backcrosses can result in progeny with chromosomes and/or chromosome segments retained from wheat. Thus, a herbicide resistance gene could migrate from wheat to jointed goatgrass. In theory, the risk of gene migration from herbicide resistant-wheat to jointed goatgrass is more likely if the gene is located on the D-genome and less likely if the gene is located on A or B genome of wheat...
November 25, 2016: Pest Management Science
https://www.readbyqxmd.com/read/27814405/the-2ns-translocation-from-aegilops-ventricosa-confers-resistance-to-the-triticum-pathotype-of-magnaporthe-oryzae
#5
C D Cruz, G L Peterson, W W Bockus, P Kankanala, J Dubcovsky, K W Jordan, E Akhunov, F Chumley, F D Baldelomar, B Valent
Wheat blast is a serious disease caused by the fungus Magnaporthe oryzae (Triticum pathotype) (MoT). The objective of this study was to determine the effect of the 2NS translocation from Aegilops ventricosa (Zhuk.) Chennav on wheat head and leaf blast resistance. Disease phenotyping experiments were conducted in growth chamber, greenhouse, and field environments. Among 418 cultivars of wheat (Triticum aestivum L.), those with 2NS had 50.4 to 72.3% less head blast than those without 2NS when inoculated with an older MoT isolate under growth chamber conditions...
2016: Crop Science
https://www.readbyqxmd.com/read/27812277/a-bioinformatics-approach-for-detecting-repetitive-nested-motifs-using-pattern-matching
#6
José R Romero, Jessica A Carballido, Ingrid Garbus, Viviana C Echenique, Ignacio Ponzoni
The identification of nested motifs in genomic sequences is a complex computational problem. The detection of these patterns is important to allow the discovery of transposable element (TE) insertions, incomplete reverse transcripts, deletions, and/or mutations. In this study, a de novo strategy for detecting patterns that represent nested motifs was designed based on exhaustive searches for pairs of motifs and combinatorial pattern analysis. These patterns can be grouped into three categories, motifs within other motifs, motifs flanked by other motifs, and motifs of large size...
2016: Evolutionary Bioinformatics Online
https://www.readbyqxmd.com/read/27795677/cytological-identification-of-an-aegilops-variabilis-chromosome-carrying-stripe-rust-resistance-in-wheat
#7
Laibin Zhao, Shunzong Ning, Jianjun Yu, Ming Hao, Lianquan Zhang, Zhongwei Yuan, Youliang Zheng, Dengcai Liu
Aegilops variabilis (UUS(v)S(v)), an important sources for wheat improvement, originated from chromosome doubling of a natural hybrid between Ae. umbellulata (UU) with Ae. longissima (S(l)S(l)). The Ae. variabilis karyotype was poorly characterized by fluorescent in situ hybridization (FISH). The FISH probe combination of pSc119.2, pTa71 and pTa-713 identified each of the 14 pairs of Ae. variabilis chromosomes. Our FISH ideogram was further used to detect an Ae. variabilis chromosome carrying stripe rust resistance in the background of wheat lines developed from crosses of the stripe rust susceptible bread wheat cultivar Yiyuan 2 with a resistant Ae...
September 2016: Breeding Science
https://www.readbyqxmd.com/read/27777460/transfer-of-hmw-glutenin-subunits-from-aegilops-kotschyi-to-wheat-through-radiation-hybridization
#8
Jasmeet Singh, Imran Sheikh, Prachi Sharma, Satish Kumar, Shailender Kumar Verma, Rahul Kumar, Priyanka Mathpal, Sundip Kumar, Pritesh Vyas, H S Dhaliwal
High molecular weight glutenin subunits (HMWGS) are responsible for dough elasticity and bread making quality of bread wheat. Related wild non-progenitor species, Aegilops kotschyi possesses higher molecular weight x and y glutenin subunits than the bread wheat cultivars. A wheat-Aegilops substitution line with 1U chromosome was used for the transfer of (HMWGS) of 1U to wheat by using pollen radiation hybridization approach. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling showed different patterns of allelic variations with either the presence or absence of HMWGS, Glu-1A (1, null), Glu-1B (7, 7 + 8, 17 + 18) and Glu-1D (5 + 10, 2 + 12, null)...
September 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27765484/sequencing-and-comparative-analysis-of-aegilops-tauschii-chromosome-arm-3ds-reveal-rapid-evolution-of-triticeae-genomes
#9
Jingzhong Xie, Naxin Huo, Shenghui Zhou, Yi Wang, Guanghao Guo, Karin R Deal, Shuhong Ouyang, Yong Liang, Zhenzhong Wang, Lichan Xiao, Tingting Zhu, Tiezhu Hu, Vijay Tiwari, Jianwei Zhang, Hongxia Li, Zhongfu Ni, Yingyin Yao, Huiru Peng, Shengli Zhang, Olin D Anderson, Patrick E McGuire, Jan Dvorak, Ming-Cheng Luo, Zhiyong Liu, Yong Q Gu, Qixin Sun
Bread wheat (Triticum aestivum, AABBDD) is an allohexaploid species derived from two rounds of interspecific hybridizations. A high-quality genome sequence assembly of diploid Aegilops tauschii, the donor of the wheat D genome, will provide a useful platform to study polyploid wheat evolution. A combined approach of BAC pooling and next-generation sequencing technology was employed to sequence the minimum tiling path (MTP) of 3176 BAC clones from the short arm of Ae. tauschii chromosome 3 (At3DS). The final assembly of 135 super-scaffolds with an N50 of 4...
October 5, 2016: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/27755540/chromosome-specific-substitution-lines-of-aegilops-geniculata-alter-parameters-of-bread-making-quality-of-wheat
#10
Monika Garg, Hisashi Tsujimoto, Raj Kumar Gupta, Aman Kumar, Navneet Kaur, Rohit Kumar, Venkatesh Chunduri, Nand Kishor Sharma, Meenakshi Chawla, Saloni Sharma, Jaspreet Kaur Mundey
Wheat cultivars with wide introgression have strongly impacted global wheat production. Aegilops geniculata (MgUg) is an important wild relative with several useful traits that can be exploited for wheat improvement. Screening of Ae. geniculata addition lines indicated a negative effect of 1Ug and the positive effect of 1Mg chromosome on wheat dough strength. Negative effect of 1Ug is probably associated with variation in number and position of the tripeptide repeat motif in the high molecular weight glutenin (HMW-G) gene...
2016: PloS One
https://www.readbyqxmd.com/read/27743251/similarities-and-differences-in-the-nuclear-genome-organization-within-pooideae-species-revealed-by-comparative-genomic-in-situ-hybridization-gish
#11
Joanna Majka, Maciej Majka, Michał Kwiatek, Halina Wiśniewska
In this paper, we highlight the affinity between the genomes of key representatives of the Pooideae subfamily, revealed at the chromosomal level by genomic in situ hybridization (GISH). The analyses were conducted using labeled probes from each species to hybridize with chromosomes of every species used in this study based on a "round robin" rule. As a result, the whole chromosomes or chromosome regions were distinguished or variable types of signals were visualized to prove the different levels of the relationships between genomes used in this study...
October 14, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27742820/piece-2-0-an-update-for-the-plant-gene-structure-comparison-and-evolution-database
#12
Yi Wang, Ling Xu, Roger Thilmony, Frank M You, Yong Q Gu, Devin Coleman-Derr
PIECE (Plant Intron Exon Comparison and Evolution) is a web-accessible database that houses intron and exon information of plant genes. PIECE serves as a resource for biologists interested in comparing intron-exon organization and provides valuable insights into the evolution of gene structure in plant genomes. Recently, we updated PIECE to a new version, PIECE 2.0 (http://probes.pw.usda.gov/piece or http://aegilops.wheat.ucdavis.edu/piece). PIECE 2.0 contains annotated genes from 49 sequenced plant species as compared to 25 species in the previous version...
October 13, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27695116/characterization-and-expression-analysis-of-phytoene-synthase-from-bread-wheat-triticum-aestivum-l
#13
Flowerika, Anshu Alok, Jitesh Kumar, Neha Thakur, Ashutosh Pandey, Ajay Kumar Pandey, Santosh Kumar Upadhyay, Siddharth Tiwari
Phytoene synthase (PSY) regulates the first committed step of the carotenoid biosynthetic pathway in plants. The present work reports identification and characterization of the three PSY genes (TaPSY1, TaPSY2 and TaPSY3) in wheat (Triticum aestivum L.). The TaPSY1, TaPSY2, and TaPSY3 genes consisted of three homoeologs on the long arm of group 7 chromosome (7L), short arm of group 5 chromosome (5S), and long arm of group 5 chromosome (5L), respectively in each subgenomes (A, B, and D) with a similarity range from 89% to 97%...
2016: PloS One
https://www.readbyqxmd.com/read/27665367/the-eyespot-resistance-genes-pch1-and-pch2-of-wheat-are-not-homoeoloci
#14
M Pasquariello, J Ham, C Burt, J Jahier, S Paillard, C Uauy, P Nicholson
Phenotyping and mapping data reveal that chromosome intervals containing eyespot resistance genes Pch1 and Pch2 on 7D and 7A, respectively, do not overlap, and thus, these genes are not homoeloci. Eyespot is a stem-base fungal disease of cereals growing in temperate regions. Two main resistances are currently available for use in wheat. Pch1 is a potent single major gene transferred to wheat from Aegilops ventricosa and located on the distal end of chromosome 7D. Pch2, a moderate resistance deriving from Cappelle Desprez, is located at the end of 7AL...
September 24, 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/27659328/molecular-cloning-characterization-and-expression-of-wag-2-alternative-splicing-transcripts-in-developing-spikes-of-aegilops-tauschii
#15
Shuhong Wei
WAG-2 is a C-class MADS-box gene, which is orthologous to AGAMOUS (AG) in Arabidopsis. The AG group C-class MADS-box genes are involved in stamen and pistil identity. In this study, two WAG-2 transcripts, namely, WAG-2f and WAG- 2g, were isolated and characterized from Aegilops tauschii. The open reading frames of WAG-2f and WAG-2g were 825 and 822 bp, respectively, encoding 275 and 274 amino acid residues. BLAST searches of partial WAG-2 genomic sequence against the draft sequence of Ae. tauschii genome database revealed the complex structure of WAG-2 gene, which consisted of seven exons and six introns...
September 2016: Journal of Genetics
https://www.readbyqxmd.com/read/27611704/high-transferability-of-homoeolog-specific-markers-between-bread-wheat-and-newly-synthesized-hexaploid-wheat-lines
#16
Deying Zeng, Jiangtao Luo, Zenglin Li, Gang Chen, Lianquan Zhang, Shunzong Ning, Zhongwei Yuan, Youliang Zheng, Ming Hao, Dengcai Liu
Bread wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) has a complex allohexaploid genome, which makes it difficult to differentiate between the homoeologous sequences and assign them to the chromosome A, B, or D subgenomes. The chromosome-based draft genome sequence of the 'Chinese Spring' common wheat cultivar enables the large-scale development of polymerase chain reaction (PCR)-based markers specific for homoeologs. Based on high-confidence 'Chinese Spring' genes with known functions, we developed 183 putative homoeolog-specific markers for chromosomes 4B and 7B...
2016: PloS One
https://www.readbyqxmd.com/read/27581540/fine-mapping-of-the-stem-rust-resistance-gene-srta10187
#17
Andrew T Wiersma, Linda K Brown, Elizabeth I Brisco, Tiffany L Liu, Kevin L Childs, Jesse A Poland, Sunish K Sehgal, Eric L Olson
SrTA10187 was fine-mapped to a 1.1 cM interval, candidate genes were identified in the region of interest, and molecular markers were developed for marker-assisted selection and Sr gene pyramiding. Stem rust (Puccinia graminis f. sp. tritici, Pgt) races belonging to the Ug99 (TTKSK) race group pose a serious threat to global wheat (Triticum aestivum L.) production. To improve Pgt host resistance, the Ug99-effective resistance gene SrTA10187 previously identified in Aegilops tauschii Coss. was introgressed into wheat, and mapped to the short arm of wheat chromosome 6D...
August 31, 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/27562405/beta-amylase-gene-variability-in-introgressive-wheat-lines
#18
Maksym Antonyuk, Anastasiia Navalikhina, Tamara Ternovska
Variability of the beta-amylase gene in bread wheat, artificial amphidiploids, and derived introgression wheat lines was analyzed. Variation in homeologous beta-amylase sequences caused by the presence of MITE (Miniature Inverted-Repeat Transposable Element) and its footprint has been identified in bread wheat. The previously unknown location of MITE in Triticum urartu and T. aestivum L. beta-amylase gene has been found. These species have a MITE sequence in the third intron of beta-amylase, as opposed to Aegilops comosa and a number of other Triticeae species, which have it in the fourth intron...
August 25, 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/27561724/genome-wide-characterization-of-microsatellites-in-triticeae-species-abundance-distribution-and-evolution
#19
Pingchuan Deng, Meng Wang, Kewei Feng, Licao Cui, Wei Tong, Weining Song, Xiaojun Nie
Microsatellites are an important constituent of plant genome and distributed across entire genome. In this study, genome-wide analysis of microsatellites in 8 Triticeae species and 9 model plants revealed that microsatellite characteristics were similar among the Triticeae species. Furthermore, genome-wide microsatellite markers were designed in wheat and then used to analyze the evolutionary relationship of wheat and other Triticeae species. Results displayed that Aegilops tauschii was found to be the closest species to Triticum aestivum, followed by Triticum urartu, Triticum turgidum and Aegilops speltoides, while Triticum monococcum, Aegilops sharonensis and Hordeum vulgare showed a relatively lower PCR amplification effectivity...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27560650/genome-wide-association-study-of-drought-related-resistance-traits-in-aegilops-tauschii
#20
Peng Qin, Yu Lin, Yaodong Hu, Kun Liu, Shuangshuang Mao, Zhanyi Li, Jirui Wang, Yaxi Liu, Yuming Wei, Youliang Zheng
The D-genome progenitor of wheat (Triticum aestivum), Aegilops tauschii, possesses numerous genes for resistance to abiotic stresses, including drought. Therefore, information on the genetic architecture of A. tauschii can aid the development of drought-resistant wheat varieties. Here, we evaluated 13 traits in 373 A. tauschii accessions grown under normal and polyethylene glycol-simulated drought stress conditions and performed a genome-wide association study using 7,185 single nucleotide polymorphism (SNP) markers...
July 2016: Genetics and Molecular Biology
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