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https://www.readbyqxmd.com/read/28731316/phytochemical-analysis-of-myrtus-communis-plant-conventional-versus-microwave-assisted-extraction-procedures
#1
Nadia Bouaoudia-Madi, Lila Boulekbache-Makhlouf, Nabil Kadri, Farid Dahmoune, Hocine Remini, Sofiane Dairi, Sonia Oukhmanou-Bensidhoum, Khodir Madani
Background Myrtle (Myrtus communis L) may constitute an interesting dietary source of health protective compounds. Microwave-assisted extraction (MAE) of total phenolic compounds (TPC) from myrtle leaf, stems, pericarp, and seeds was studied and the results were compared with those of the conventional method extraction (CME) in terms of extraction time. Methods Extraction yield/efficiency and antioxidant activity were measured using radical scavenging assay (DPPH•) and reducing power. Results The results show that the MAE was higher in terms of saving energy, extraction time (62 s) and extraction efficiency of bioactive compound compared to CME (2 h)...
June 10, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28725039/surface-chemistry-and-germination-improvement-of-quinoa-seeds-subjected-to-plasma-activation
#2
A Gómez-Ramírez, C López-Santos, M Cantos, J L García, R Molina, J Cotrino, J P Espinós, A R González-Elipe
Plasma treatment is recognized as a suitable technology to improve germination efficiency of numerous seeds. In this work Quinoa seeds have been subjected to air plasma treatments both at atmospheric and low pressure and improvements found in germination rate and percentage of success. Seed water uptake by exposure to water vapor, although slightly greater for plasma treated seeds, did not justify the observed germination improvement. To identify other possible factors contributing to germination, the chemical changes experienced by outer parts of the seed upon plasma exposure have been investigated by X-ray photoemission spectroscopy (XPS) and scanning electron microscopy (SEM-EDX)...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724949/overexpression-of-slpre2-an-atypical-bhlh-transcription-factor-affects-plant-morphology-and-fruit-pigment-accumulation-in-tomato
#3
Zhiguo Zhu, Guoping Chen, Xuhu Guo, Wencheng Yin, Xiaohui Yu, Jingtao Hu, Zongli Hu
The basic helix-loop-helix (bHLH) proteins are a large family of transcription factors that control various developmental processes in eukaryotes, but the biological roles of most bHLH proteins are not very clear, especially in tomato. In this study, a PRE-like atypical bHLH gene was isolated and designated as SlPRE2 in tomato. SlPRE2 was highly expressed in immature-green fruits, moderately in young leaves, flowers, and mature-green fruits. To further research the function of SlPRE2, a 35 S:PRE2 binary vector was constructed and transformed into wild type tomato...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724331/randomized-double-blinded-pilot-clinical-study-of-the-antidiabetic-activity-of-balanites-aegyptiaca-and-uplc-esi-ms-ms-identification-of-its-metabolites
#4
Hend Rashad, Fateheya M Metwally, Shahira M Ezzat, Maha M Salama, Adel Hasheesh, Amira Abdel Motaal
CONTEXT: Balanites aegyptiaca Del. (Zygophyllaceae) fruits are traditionally known for the treatment of hyperglycaemia. Several in vitro and in vivo studies proposed some mechanisms of action. However, clinical trials in human beings were never reported to date. OBJECTIVES: To investigate the antidiabetic efficacy of the 70% ethanol extract of the pericarps of B. aegyptiaca (BE) within a nutritional intervention in elderly people. MATERIALS AND METHODS: Ultra-performance electrospray ionization-mass spectroscopy (UPLC-ESI-MS/MS) analysis was used for metabolic profiling of BE which was incorporated in hard gelatine capsules (400 mg/day) and tested on 30 type 2 diabetes (T2D) Egyptian patients for 8 weeks...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28720977/effect-of-integration-of-oxalic-acid-and-hot-water-treatments-on-postharvest-quality-of-rambutan-nephelium-lappaceum-l-cv-anak-sekolah-under-modified-atmosphere-packaging
#5
Ahmad Faiz Ahmad Hafiz, Yeoh Wei Keat, Asgar Ali
The shelf life of rambutan is often limited due to rapid water loss from the spinterns and browning of the pericarp. An integrated approach, which combined hot water treatment (HWT) (56 °C for 1 min), oxalic acid (OA) dip (10% for 10 min) and modified atmosphere packaging (MAP), was used to study their effectiveness on the quality of rambutan during storage (10 °C, 90-95% relative humidity). Significant differences were observed in rambutan quality with the combination of MAP + HWT + OA after 20 days of storage...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720800/itraq-based-quantitative-proteomic-analysis-reveals-alterations-in-the-metabolism-of-actinidia-arguta
#6
Miaomiao Lin, Jinbao Fang, Xiujuan Qi, Yukuo Li, Jinyong Chen, Leiming Sun, Yunpeng Zhong
Actinidia arguta 'Tianyuanhong' is a new kiwifruit variety with an all-red pericarp and pulp, in contrast to the all-green pulp of A. arguta 'Yongfengyihao'. Transcriptome profile analysis of fruit color has been reported, however, the metabolic mechanisms producing red flesh remain unknown, and it is unclear why the pulp of 'Tianyuanhong' is red rather than green. Herein, we identified differences between the proteomes of two A. arguta cultivars with different fruit color by using iTRAQ-based quantitative proteomic methods during the stage of color change...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28702654/direct-and-indirect-measurements-of-enhanced-phenolic-bioavailability-from-litchi-pericarp-procyanidins-by-lactobacillus-casei-01
#7
Shuyi Li, Xiaopeng Li, Avi Shpigelman, Jose M Lorenzo, Domenico Montesano, Francisco J Barba
Litchi pericarp procyanidins (LPP) are dietary supplements with high antioxidant activity, but poor oral bioavailability and efficacy. Lactobacillus casei (L. casei-01) can transform flavan-3-ols from litchi pericarp and increase their antioxidant ability; thus, L. casei-01 with LPP was administered to rats for four and eight weeks to study the effect of such a combination on metabolic parameters and on phase II metabolism and detoxification pathways in the liver as an indirect measure for phenolic bioavailability...
July 12, 2017: Food & Function
https://www.readbyqxmd.com/read/28700827/compatible-rhizosphere-competent-microbial-consortium-add-value-to-nutritional-quality-in-edible-parts-of-chickpea
#8
Sudheer KUmar Yadav, Surendra Singh, Harikesh Bahadur Singh, Birinchi Kumar Sarma
Chickpea is used as a high energy and protein source in human and livestock's diets. Moreover, chickpea straw can be used as alternative of forage in ruminant diets. The present study evaluates the effect of beneficial microbial inoculation on enhancing the nutritional values in edible parts of chickpea. Two rhizosphere competent compatible microbes (Pseudomonas fluorescens OKC and Trichoderma asperellum T42) were selected and applied to seeds either individually or in consortium before sowing. Chickpea seeds treated with the microbes showed enhanced plant growth (88...
July 12, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28700755/the-dead-seed-coat-functions-as-a-long-term-storage-for-active-hydrolytic-enzymes
#9
Buzi Raviv, Lusine Aghajanyan, Gila Granot, Vardit Makover, Omer Frenkel, Yitzchak Gutterman, Gideon Grafi
Seed development culminates in programmed cell death (PCD) and hardening of organs enclosing the embryo (e.g., pericarp, seed coat) providing essentially a physical shield for protection during storage in the soil. We examined the proposal that dead organs enclosing embryos are unique entities that store and release upon hydration active proteins that might increase seed persistence in soil, germination and seedling establishment. Proteome analyses of dead seed coats of Brassicaceae species revealed hundreds of proteins being stored in the seed coat and released upon hydration, many are stress-associated proteins such as nucleases, proteases and chitinases...
2017: PloS One
https://www.readbyqxmd.com/read/28675968/effects-of-a-high-o2-dynamic-controlled-atmosphere-technology-on-the-browning-of-postharvest-white-mushroom-agaricus-bisporus-in-relation-to-energy-metabolism
#10
Ling Li, Han Sun, Hiroaki Kitazawa, Xiangyou Wang
Browning is one of the main problems in senescence of mushrooms, and it is also one of the most important attributes accounting for the loss of the quality and reduction in market value. In order to study the relationship between the energy metabolism and the browning of white mushroom under high O2 dynamic-controlled atmosphere (HO-DCA), mushrooms were stored in 100% O2 (SCA1), 80% O2 + 20% CO2 (SCA2), 100% O2 for three days and then transferred into the treatment of 80% O2 + 20% CO2 (HO-DCA) at 2 ± 1 ℃ and air as control...
July 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28674553/expression-of-genes-for-si-uptake-accumulation-and-correlation-of-si-with-other-elements-in-ionome-of-maize-kernel
#11
Boris Bokor, Slavomír Ondoš, Marek Vaculík, Silvia Bokorová, Marieluise Weidinger, Irene Lichtscheidl, Ján Turňa, Alexander Lux
The mineral composition of cells, tissues, and organs is decisive for the functioning of the organisms, and is at the same time an indicator for understanding of physiological processes. We measured the composition of the ionome in the different tissues of maize kernels by element microanalysis, with special emphasis on silicon (Si). We therefore also measured the expression levels of the Si transporter genes ZmLsi1, ZmLsi2 and ZmLsi6, responsible for Si uptake and accumulation. Two weeks after pollination ZmLsi1 and ZmLsi6 genes were expressed, and expression continued until the final developmental stage of the kernels, while ZmLsi2 was not expressed...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28659942/identification-of-two-new-mechanisms-that-regulate-fruit-growth-by-cell-expansion-in-tomato
#12
Constance Musseau, Daniel Just, Joana Jorly, Frédéric Gévaudant, Annick Moing, Christian Chevalier, Martine Lemaire-Chamley, Christophe Rothan, Lucie Fernandez
Key mechanisms controlling fruit weight and shape at the levels of meristem, ovary or very young fruit have already been identified using natural tomato diversity. We reasoned that new developmental modules prominent at later stages of fruit growth could be discovered by using new genetic and phenotypic diversity generated by saturated mutagenesis. Twelve fruit weight and tissue morphology mutants likely affected in late fruit growth were selected among thousands of fruit size and shape EMS mutants available in our tomato EMS mutant collection...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28649256/evolutionary-insights-based-on-snp-haplotypes-of-red-pericarp-grain-size-and-starch-synthase-genes-in-wild-and-cultivated-rice
#13
Nisha Singh, Balwant Singh, Vandna Rai, Sukhjeet Sidhu, Ashok K Singh, Nagendra K Singh
The origin and domestication of rice has been a subject of considerable debate in the post-genomic era. Rice varieties have been categorized based on isozyme and DNA markers into two broad cultivar groups, Indica and Japonica. Among other well-known cultivar groups Aus varieties are closer to Indica and Aromatic varieties including Basmati are closer to Japonica, while deep-water rice varieties share kinship to both Indica and Japonica cultivar groups. Here, we analyzed haplotype networks and phylogenetic relationships in a diverse set of genotypes including Indian Oryza nivara/Oryza rufipogon wild rice accessions and representative varieties of four rice cultivar groups based on pericarp color (Rc), grain size (GS3) and eight different starch synthase genes (GBSSI, SSSI, SSIIa, SSIIb, SSIIIa, SSIIIb, SSIVa, and SSIVb)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28641010/alpha-mangostin-reverses-multidrug-resistance-by-attenuating-the-function-of-the-multidrug-resistance-linked-abcg2-transporter
#14
Chung-Pu Wu, Sung-Han Hsiao, Megumi Murakami, Yu-Jen Lu, Yan-Qing Li, Yang-Hui Huang, Tai-Ho Hung, Suresh V Ambudkar, Yu-Shan Wu
The ATP-binding cassette (ABC) drug transporter ABCG2 can actively efflux a wide variety of chemotherapeutic agents out of cancer cells and subsequently reduce the intracellular accumulation of these drugs. Therefore, the overexpression of ABCG2 often contributes to the development of multidrug resistance (MDR) in cancer cells, which is one of the major obstacles to successful cancer chemotherapy. Moreover, ABCG2 is highly expressed in various tissues including the intestine and blood-brain barrier (BBB), limiting the absorption and bioavailability of many therapeutic agents...
June 22, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28624092/study-on-the-effects-of-wheat-bran-incorporation-on-water-mobility-and-biopolymer-behavior-during-bread-making-and-storage-using-time-domain-1-h-nmr-relaxometry
#15
S Hemdane, P J Jacobs, G M Bosmans, J Verspreet, J A Delcour, C M Courtin
Water binding is suggested to be key in the deleterious effect of wheat bran on bread quality. This study investigates water mobility and biopolymer behavior during bran-rich bread making and storage, using (1)H NMR. Coarse, ground, and pericarp-enriched bran were incorporated in bread dough, and their impact on freshly baked and stored bread properties was assessed. Compared to wheat flour control dough, bran incorporation resulted in a progressive immobilization of water during dough resting, which could be linked to changes in evolution of dough height during fermentation and oven rise...
December 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28624091/study-of-biopolymer-mobility-and-water-dynamics-in-wheat-bran-using-time-domain-1-h-nmr-relaxometry
#16
S Hemdane, P J Jacobs, G M Bosmans, J Verspreet, J A Delcour, C M Courtin
In this study, the molecular mobility of water and biopolymers in coarse, ground, and pericarp-enriched (PE) wheat bran and refined flour was investigated using time-domain proton nuclear magnetic resonance relaxometry, and related to their hydration properties. Several specific proton populations were present in the bran samples but not in flour. These populations were mainly assigned to protons of bran-related compounds such as arabinoxylan, cellulose, and lipids. All bran samples showed similar proton distributions at a 44% moisture level, although the chemical composition of coarse/ground bran and PE bran differed...
December 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28602011/differences-in-the-fruit-structure-and-the-location-and-content-of-bioactive-substances-in-viburnum-opulus-and-viburnum-lantana-fruits
#17
Agata Konarska, Marcin Domaciuk
Many Viburnum species are popular ornamental shrubs and, simultaneously, highly valued medicinal plants as a source of many bioactive compounds, including antioxidants. Viburnum bark, flowers, and fruits are widely used in traditional and folk medicine, and the fruits of some species are used as cooking ingredients. The knowledge of the microstructure of Viburnum fruits and the accumulation sites of bioactive substances in these organs is rather poor. Comparative analyses of the microstructure of ripe Viburnum opulus and Viburnum lantana drupes were carried out using light, scanning, and transmission electron microscopes...
June 10, 2017: Protoplasma
https://www.readbyqxmd.com/read/28592557/comparative-mapping-of-seed-dormancy-loci-between-tropical-and-temperate-ecotypes-of-weedy-rice-oryza-sativa-l
#18
Lihua Zhang, Jieqiong Luo, Michael E Foley, Xing-You Gu
Genotypic variation at multiple loci for seed dormancy (SD) contributes to plant adaptation to diverse ecosystems. Weedy rice (Oryza sativa) was used as a model to address the similarity of SD genes between distinct ecotypes. A total of 12 quantitative trait loci for SD were identified in a primary and two advanced backcross populations derived from a temperate ecotype of weedy rice (34.3°N Lat.). Nine (75%) of the 12 loci were mapped to the same positions as those identified from a tropical ecotype of weedy rice (7...
June 7, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28587348/hepatoprotective-effects-of-litchi-litchi-chinensis-procyanidin-a2-on-carbon-tetrachloride-induced-liver-injury-in-icr-mice
#19
Lih-Geeng Chen, Cheng-Wei Chang, Jwu-Guh Tsay, Brian Bor-Chun Weng
Drug tolerance, lacking liver regenerative activity and inconclusive inhibition of steatosis and cirrhosis by silymarin treatment during chronic liver injury have increased the demand for novel alternative or synergistic treatments for liver damage. Litchi fruit is abundant in polyphenolic compounds and is used in traditional Chinese medicine for treatments that include the strengthening of hepatic and pancreatic functions. Unique polyphenolic compounds obtained from litchi pericarp extract (LPE) were studied in vitro and in vivo for hepatoprotection...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28582759/%C3%AE-mangostin-inhibits-dmba-tpa-induced-skin-cancer-through-inhibiting-inflammation-and-promoting-autophagy-and-apoptosis-by-regulating-pi3k-akt-mtor-signaling-pathway-in-mice
#20
Fei Wang, Hongxia Ma, Zhaoguo Liu, Wei Huang, Xiaojing Xu, Xuemei Zhang
Skin cancer is the most common form of cancer responsible for considerable morbidity and mortality, the treatment progress of which remains slow though. Therefore, studies identifying anti-skin cancer agents that are innocuous are urgently needed. α-Mangostin, a natural product isolated from the pericarp of mangosteen fruit, has potent anti-cancer activity. However, its role in skin cancer remains unclear. The aim of this study was to evaluate the treatment effect of α-mangostin on skin tumorigenesis induced by 9,10-dimethylbenz[a]anthracene (DMBA)/TPA in mice and the potential mechanism...
August 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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