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https://www.readbyqxmd.com/read/28212656/using-a-vector-pool-containing-variable-strength-promoters-to-optimize-protein-production-in-yarrowia-lipolytica
#1
Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud, Christophe Leplat
BACKGROUND: The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and the hybrid hp4d promoter. Recently, new hp4d-inspired promoters have been created that couple various numbers of UAS1 tandem elements with the minimal LEU2 promoter or the TEF promoter. Three different protein-secretion signaling sequences can be used: preLip2, preXpr2, and preSuc2...
February 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28207250/dna-product-formation-in-female-sprague-dawley-rats-following-polyhalogenated-aromatic-hydrocarbon-phah-exposure
#2
Lina Gao, Esra Mutlu, Leonard B Collins, Nigel J Walker, Hadley J Hartwell, James R Olson, Wei Sun, Avram Gold, Louise M Ball, James A Swenberg
DNA oxidation damage has been regarded as one of the possible mechanisms for the hepatic carcinogenesis of dioxin-like compounds (DLCs). In this study, we evaluated the toxic equivalency factor (TEF) from the standpoint of induced DNA oxidation products and their relationship to toxicity and carcinogenicity. Nine DNA oxidation products were analyzed in the liver of female Sprague-Dawley rats exposed to 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) alone or the tertiary mixture of TCDD, 3,3',4,4',5-pentachlorobiphenyl (PCB 126), and 2,3,4,7,8-pentachlorodibenzofuran (PeCDF) by gavage for 14, 31, and 53 weeks (5 days/week) by LC-MS/MS: 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo); 1,N(6)-etheno-2'-deoxyadenosine (1,N(6)-εdAdo); N(2),3-ethenoguanine (N(2),3-εG); 7-(2-oxoethly)guanine (7-OEG); 1,N(2)-etheno-2'-deoxyguanosine (1,N(2)-εdGuo); malondialdehyde (M1dGuo); acrolein (AcrdGuo); crotonaldehyde (CrdGuo); and 4-hydroxynonenal (HNEdGuo) derived 2'-deoxyguanosine adducts...
February 16, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28205736/p-15-a-specialized-multi-disciplinary-clinic-for-children-with-esophageal-atresia-tracheoesophageal-fistula-ea-tef
#3
M C Chiang, V S Scaini, P M Marcon, P C Chiu
No abstract text is available yet for this article.
April 1, 2016: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
https://www.readbyqxmd.com/read/28205733/p-17-incidence-of-malnutrition-in-children-with-ea-tef-and-the-role-of-a-multidisciplinary-clinic-in-improving-feeding-and-nutritional-outcomes
#4
J M Menzies, J H Hughes, C C Clarkson, V V Varjavandi, A J Jiwane, S A Adams, C W Wu, M S Soma, Y B Belessis, U K Krishnan
No abstract text is available yet for this article.
April 1, 2016: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
https://www.readbyqxmd.com/read/28205730/p-35-gastric-metaplasia-in-patients-with-esophageal-atresia-tracheo-esophageal-fistula-ea-tef-aged-less-than-10-years
#5
H H Hsieh, A Frenette, A Aspirot, C Faure
No abstract text is available yet for this article.
April 1, 2016: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
https://www.readbyqxmd.com/read/28205717/p-41-what-mothers-think-of-communication-about-their-child-with-tracheoesophageal-fistula-tef
#6
J M Platt, I M Mitchell, L Fairservice, M Soles, J A M Bailey, C Bjornson, M Brindle
No abstract text is available yet for this article.
April 1, 2016: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
https://www.readbyqxmd.com/read/28205710/p-39-endoscopic-multidisciplinary-team-for-ea-tef-patients-a-pilot-study
#7
D Lévesque, D Zielinski, S J Daniel
No abstract text is available yet for this article.
April 1, 2016: Diseases of the Esophagus: Official Journal of the International Society for Diseases of the Esophagus
https://www.readbyqxmd.com/read/28199040/isotopic-half-life-and-enrichment-factor-in-two-species-of-european-freshwater-fish-larvae-an-experimental-approach
#8
Adrien Latli, Nicolas Sturaro, Nelson Desjardin, Loïc N Michel, William Otjacques, Gilles Lepoint, Patrick Kestemont
RATIONALE: Stable isotope ratios of carbon and nitrogen are valuable tools for field ecologists to use to analyse animal diets. However, the application of these tools requires knowledge of the tissue enrichment factor (TEF) and half-life (HL). We experimentally compared TEF and HL in 2 freshwater fish larvae. We hypothesised that chub had a better growth/tissue replacement ratio than roach, due to the use of a food closer to their natural diet. METHODS: We determined the isotopic HL, the TEF and the contribution of growth or metabolic tissue replacement to dynamic isotopic incorporation...
February 15, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28190769/paf1-has-distinct-roles-in-transcription-elongation-and-differential-transcript-fate
#9
Harry Fischl, Françoise S Howe, Andre Furger, Jane Mellor
RNA polymerase II (Pol2) movement through chromatin and the co-transcriptional processing and fate of nascent transcripts is coordinated by transcription elongation factors (TEFs) such as polymerase-associated factor 1 (Paf1), but it is not known whether TEFs have gene-specific functions. Using strand-specific nucleotide resolution techniques, we show that levels of Paf1 on Pol2 vary between genes, are controlled dynamically by environmental factors via promoters, and reflect levels of processing and export factors on the encoded transcript...
January 31, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28166998/isolated-tracheoesophageal-fistula-versus-esophageal-atresia-early-morbidity-and-short-term-outcome-a-single-institution-series
#10
R B Tröbs, W Finke, M Bahr, C Roll, M Nissen, M R Vahdad, G Cernaianu
PURPOSE: We compared the postnatal course, morbidity and early results after repair for cases of isolated or "pure" TEF with those for cases of esophageal atresia (EA) with distal tracheoesophageal fistula (TEF). METHODS: Twenty-four consecutive infants were divided into two groups: isolated TEF [TEF group] (n = 5) and EA with distal TEF [EA group] (n = 19). RESULTS: A high rate of prematurity (29%) and major cardiac and other surgically-relevant malformations (0...
March 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28164003/ingested-metallic-spool-a-rare-cause-of-acquired-tracheoesophageal-fistula
#11
Imran Hashim, Nabila Talat
Foreign body (FB) ingestion is a common problem in children. Prolonged impaction of FB in esophagus may result in tracheoesophageal fistula (TEF). A 6-year-old girl presented with progressive dysphagia and recurrent chest infections. No history of FB ingestion was given by parents. Further investigations revealed FB (spool) in cervical esophagus. Patient was successfully managed by surgery through trans-cervical approach.
January 2017: APSP Journal of Case Reports
https://www.readbyqxmd.com/read/28144844/surveillance-in-patients-with-esophageal-atresia-tracheoesophageal-fistula
#12
REVIEW
Arunjot Singh, William Middlesworth, Julie Khlevner
PURPOSE OF REVIEW: Esophageal atresia/tracheoesophageal fistula (EA/TEF) is a congenital aerodigestive anomaly with high survival rates after surgical repair. Care should now be focused on prevention of long-term complications using appropriate surveillance techniques. RECENT FINDINGS: The incidence of gastroesophageal reflux disease (GERD) is high in patients with EA/TEF. Consequences of untreated GERD include esophagitis, strictures, and Barrett esophagus. Subjective symptoms are an unreliable indicator of presence or severity of GERD, and therefore, diagnostic testing is needed to assess esophageal heath and monitor the effectiveness of anti-reflux treatment...
January 2017: Current Gastroenterology Reports
https://www.readbyqxmd.com/read/28143945/differential-regulation-of-cyclin-e-by-yorkie-scalloped-signaling-in-organ-development
#13
Zhiqiang Shu, Wu-Min Deng
Tissue integrity and homeostasis are accomplished through strict spatial and temporal regulation of cell growth and proliferation during development. Various signaling pathways have emerged as major growth regulators across metazoans; yet, how differential growth within a tissue is spatiotemporally coordinated remains largely unclear. Here, we report a role of a growth modulator Yorkie (Yki), the Drosophila homolog of Yes-associated protein (YAP), differentially regulates its targets in Drosophila wing imaginal discs, whereby Yki interacts with its transcriptional partner, Scalloped (Sd), the homolog of the TEAD/TEF family transcription factor in mammals, to control an essential cell-cycle regulator Cyclin E (CycE)...
January 30, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28134049/outcomes-after-orthopedic-trauma
#14
Michael Suk, Monica Daigl, Richard E Buckley, Dean G Lorich, David L Helfet, Beate Hanson
BACKGROUND: Clinical orthopedic research needs better ability to assess patient expectations with regard to orthopedic trauma surgery outcomes. The aim of this study was to investigate to which extent patient expectations prior to surgery could be met after surgery. METHODS: Patients (≥18 years) with surgical ankle fractures were prospectively recruited at 5 orthopedic trauma clinics in the United States (USA), Canada, and Brazil and followed up for 12 months...
January 2017: Journal of Orthopaedic Surgery
https://www.readbyqxmd.com/read/28122791/repair-of-challenging-non-malignant-tracheo-or-broncho-oesophageal-fistulas-by-extrathoracic-muscle-flaps
#15
Petra Rosskopfova, Jean Yannis Perentes, Markus Schäfer, Thorsten Krueger, Alban Lovis, Gian Dorta, Moira Baeriswyl, Hans-Beat Ris, Michel Gonzalez
OBJECTIVES: Evaluation of complex, acquired, non-malignant tracheo/broncho-oesophageal fistulas (TEF) repaired by extrathoracic pedicled muscle flaps that were, in addition to their interposition between the airways and the gastro-intestinal tract, patched into gastro-intestinal or airway defects if primary closure seemed risky. METHODS: A single institution experience of patients treated between 2003 and 2015. Twenty-two patients required TEF repair following oesophageal surgery (18), Boerhaave syndrome (1), chemotherapy for mediastinal lymphoma (1), carinal resection and irradiation (1) and laryngectomy (1); 64% of them underwent prior radio- or chemotherapy and 50% prior airway or oesophageal stenting...
January 25, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28110246/fe-fe2o3-promoted-electrochemical-mineralization-of-atrazine-via-a-triazinon-ring-opening-mechanism
#16
Xing Ding, Shengyao Wang, Wanqiu Shen, Yi Mu, Li Wang, Hao Chen, Lizhi Zhang
In this study, an electrochemical/electro-Fenton oxidation (EC/EF) system was designed to degrade atrazine, by utilizing boron-doped diamond (BDD) and Fe@Fe2O3 core-shell nanowires loaded active carbon fiber (Fe@Fe2O3/ACF) as the anode and the cathode, respectively. This EC/EF system exhibited much higher degradation rate, decholorination and mineralization efficiency of atrazine than the electrochemical (EC) and electrochemical/traditional electro-Fenton (EC/TEF) oxidation counterpart systems without Fe@Fe2O3 core-shell nanowires...
January 16, 2017: Water Research
https://www.readbyqxmd.com/read/28105337/development-of-a-polymerase-chain-reaction-restriction-fragment-length-polymorphism-method-for-identification-of-the-fusarium-genus-using-the-transcription-elongation-factor-1%C3%AE-gene
#17
Majid Zarrin, Farzaneh Ganj, Sama Faramarzi
Fusarium species are well-known plant pathogens and food contaminants that have also appeared as one of the most important groups of medically significant fungi. The sequences of the translation elongation factor (TEF)-1α gene have been broadly employed for species detection. A total of 50 strains of Fusarium spp., including environmental, clinical and reference isolates were used for the current study. The primer sets, Fu3f and Fu3r, were used to amplify an ~420-bp DNA fragment of the TEF-1α gene. Double digestion with two restriction enzymes, XhoI and SduI was used for discrimination of the Fusarium species in the TEF-1α gene fragment...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28066602/a-novel-surgical-method-for-acquired-non-malignant-complicated-tracheoesophageal-and-bronchial-gastric-stump-fistula-the-double-patch-technique
#18
Guang Yang, Wei-Miao Li, Jin-Bo Zhao, Jian Wang, Yun-Feng Ni, Yong-An Zhou, Yong Han, Xiao-Fei Li, Xiao-Long Yan
BACKGROUND: To manage the acquired benign complicated tracheoesophageal fistula (TEF) and bronchial-gastric stump fistula (BGSF) are clinical technical challenge. The purpose of this study is to retrospectively review a surgical "double patch" technique in treating nonmalignant complicated TEF and BGSF, and then clarify the long-term curative effect of the technique. METHODS: Clinical records of 30 patients with non-malignant complicated TEF and BGSF treated by "double patch" technique in Tangdu Hospital between August 2004 and August 2014, were analyzed and summarized retrospectively...
November 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28064362/thoracoscopic-surgery-for-esophageal-atresia
#19
REVIEW
George W Holcomb
This review centers on the thoracoscopic management of esophageal atresia (EA) and distal tracheoesophageal fistula (TEF). The first thoracoscopic repair of EA was performed by Rothenberg and Lobe in Berlin in 1999 just prior to an IPEG meeting. Since that time, the largest report describing the use of thoracoscopy for EA/TEF repair came in 2005 with a multi-national, multi-institutional retrospective review from six institutions around the world. The outcomes reported were quite good and very comparable to large series of open operations that had been previously reported...
January 7, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28063029/tracheo-esophageal-fistula-tef-in-a-newborn-following-maternal-antenatal-exposure-to-olanzapine
#20
Vikas Maharshi, Indranil Banerjee, Pravesh Nagar, Harmeet Singh Rehan
There is a dearth of evidence on the safety of the use of antipsychotics during pregnancy. Olanzapine, a pregnancy category C drug, has no unequivocal evidence of harm to the fetus. Against this backdrop, we report the first case of a tracheo-esophageal fistula (TEF) in a newborn following maternal antenatal exposure to olanzapine. A 29-year-old woman with acute psychotic disorder had been treated with olanzapine for the last 7 years. Her first pregnancy, while taking olanzapine, resulted in a miscarriage at 4 months' gestation, following which she discontinued olanzapine...
December 2017: Drug Safety—Case Reports
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