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https://www.readbyqxmd.com/read/28730131/clinical-study-of-congenital-esophageal-stenosis-comparison-according-to-association-of-esophageal-atresia-and-tracheoesophageal-fistula
#1
Soo-Hong Kim, Hyun-Young Kim, Sung-Eun Jung, Seong-Cheol Lee, Kwi-Won Park
PURPOSE: Congenital esophageal atresia (CES) is a rare congenital disease. The severity of symptoms is variable; thus, diagnosis is difficult and tends to be delayed. CES is frequently accompanied by esophageal atresia (EA) with/without tracheoesophageal fistula (TEF). We investigated the characteristics of CES by reviewing our experience with CES patients and researched the differences between CES with EA-TEF and isolated CES. METHODS: A total of 31 patients underwent operations for CES were reviewed retrospectively...
June 2017: Pediatric Gastroenterology, Hepatology & Nutrition
https://www.readbyqxmd.com/read/28718991/learning-curve-for-the-thoracoscopic-repair-of-esophageal-atresia-with-tracheoesophageal-fistula
#2
Hiroomi Okuyama, Yuko Tazuke, Takehisa Ueno, Hiroaki Yamanaka, Yuichi Takama, Ryuta Saka, Noriaki Usui, Hideki Soh, Takeo Yonekura
AIM: Thoracoscopic repair (TR) of esophageal atresia with tracheoesophageal fistula (EA/TEF) remains a considerable challenge, even for the most experienced pediatric surgeons. The aim of this study is to report the outcomes of our experience with TR of EA/TEF and to determine the learning curve for this procedure. METHODS: Eleven consecutive cases that had undergone TR of EA/TEF at our institutes were included in this study. The medical charts were reviewed retrospectively...
July 18, 2017: Asian Journal of Endoscopic Surgery
https://www.readbyqxmd.com/read/28671814/preparation-and-gas-permeation-properties-of-fluorine-silica-membranes-with-controlled-amorphous-silica-structures-effect-of-fluorine-source-and-calcination-temperature-on-network-size
#3
Masakoto Kanezashi, Takuya Matsutani, Toru Wakihara, Hiroki Nagasawa, Tatsuya Okubo, Toshinori Tsuru
Triethoxyfluorosilane (TEFS), which is a pendant-type alkoxysilane with a Si-F bond, was utilized as a Si precursor for the fabrication of a molecular sieving membrane. The effect that a source of fluorine and calcination temperature exerted on network pore size and gas permeation properties was evaluated via single-gas permeation properties across a wide range of temperatures. A TEFS membrane calcined at 350 oC showed high H2 permeance (2.0 x 10-6 mol m-2 s-1 Pa-1) and high selectivity for H2 over larger molecules (H2/CF4: > 300, H2/SF6: > 18,000), indicating that this network pore size would be suitable for a H2 permselective membrane that could promote the process of methylcyclohexane (MCH) dehydrogenation to produce toluene (TOL)...
July 3, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28671160/placement-of-tracheobronchial-silicone-y-stents-multicenter-experience-and-systematic-review-of-the-literature
#4
Inderpaul Singh Sehgal, Sahajal Dhooria, Karan Madan, Vallandramam Pattabhiraman, Ravindra Mehta, Rajiv Goyal, Jayachandra Akkaraju, Ritesh Agarwal
BACKGROUND: Airway obstruction or tracheoesophageal fistula (TEF) near the tracheal carina requires placement of Y-shaped stents. Herein, we describe our multicenter experience with the placement of Dumon silicone Y-stents. We also conduct a systematic review for studies describing the deployment of airway silicone Y-stents. METHODS: This was a retrospective analysis of consecutive subjects who underwent placement of silicone Y-stents. The clinical details including the underlying diagnosis, indication for the placement of silicone Y-stents, success of stent placement, and follow-up are presented...
July 2017: Lung India: Official Organ of Indian Chest Society
https://www.readbyqxmd.com/read/28669845/dioxin-like-activity-of-brominated-dioxins-as-individual-compounds-or-mixtures-in-in-vitro-reporter-gene-assays-with-rat-and-mouse-hepatoma-cell-lines
#5
G Suzuki, M Nakamura, C Michinaka, N M Tue, H Handa, H Takigami
In vitro reporter gene assays detecting dioxin-like compounds have been developed and validated since the middle 1990's, and applied to the determination of dioxin-like activities in various samples for their risk management. Data on characterizing the potency of individual brominated dioxins and their activity in mixture with chlorinated dioxins are still limited on the cell-based assay. This study characterized the dioxin-like activities of the 32 brominated dioxins, such as polybrominated dibenzo-p-dioxins, polybrominated dibenzofurans (PBDFs), coplanar polybrominated biphenyls, mixed halogenated dibenzo-p-dioxins and dibenzofurans (PXDFs), as a sole component or in a mixture by DR-CALUX (dioxin-responsive chemically activated luciferase expression) using the rat hepatoma H4IIE cell line and XDS-CALUX (xenobiotic detection systems-chemically activated luciferase expression) assays using the mouse hepatoma H1L6...
June 29, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28667772/genotypic-diversity-in-root-endophytic-fungi-reflects-efficient-dispersal-and-environmental-adaptation
#6
Kyriaki Glynou, Tahir Ali, Sevda Haghi Kia, Marco Thines, Jose G Maciá-Vicente
Studying community structure and dynamics of plant-associated fungi is the basis for unravelling their interactions with hosts and ecosystem functions. A recent sampling revealed that only a few fungal groups, as defined by ITS sequence similarity, dominate culturable root endophytic communities of non-mycorrhizal Microthlaspi spp. plants across Europe. Strains of these fungi display a broad phenotypic and functional diversity, which suggests a genetic variability masked by ITS clustering into operational taxonomic units (OTUs)...
July 1, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28656514/is-routine-use-of-transanastomotic-tube-justified-in-the-repair-of-esophageal-atresia
#7
Sarath Kumar Narayanan, Arun Preeth Vazhiyodan, Prathap Somnath, Arun Mohanan
BACKGROUND: Transanastomotic feeding tube (TAFT) is commonly used for post-operative enteral nutrition after esophageal atresia (EA)/tracheoesophageal fistula (TEF) repairs. The purpose of this study is to analyze the therapeutic implications of avoiding a TAFT and its impact on the outcomes post-operatively. METHODS: The medical data of 33 inpatients with EA/TEF type C repair from 2009 to 2014 was retrospectively reviewed. Patients were divided into two groups for comparison based on the usage of TAFT during the repair: TAFT- (without) and TAFT+ (with) groups, without randomization...
June 27, 2017: World Journal of Pediatrics: WJP
https://www.readbyqxmd.com/read/28648292/fusarium-proliferatum-causal-agent-of-garlic-bulb-rot-in-spain-genetic-variability-and-mycotoxin-production
#8
Laura Gálvez, Monika Urbaniak, Agnieszka Waśkiewicz, Łukasz Stępień, Daniel Palmero
Fusarium proliferatum is a world-wide occurring fungal pathogen affecting several crops included garlic bulbs. In Spain, this is the most frequent pathogenic fungus associated with garlic rot during storage. Moreover, F. proliferatum is an important mycotoxigenic species, producing a broad range of toxins, which may pose a risk for food safety. The aim of this study is to assess the intraspecific variability of the garlic pathogen in Spain implied by analyses of translation elongation factor (tef-1α) and FUM1 gene sequences as well as the differences in growth rates...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28623582/analysis-of-in-vivo-chromatin-and-protein-interactions-of-arabidopsis-transcript-elongation-factors
#9
Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, Klaus D Grasser
A central step to elucidate the function of proteins commonly comprises the analysis of their molecular interactions in vivo. For nuclear regulatory proteins this involves determining protein-protein interactions as well as mapping of chromatin binding sites. Here, we present two protocols to identify protein-protein and chromatin interactions of transcript elongation factors (TEFs) in Arabidopsis. The first protocol (Subheading 3.1) describes protein affinity-purification coupled to mass spectrometry (AP-MS) that utilizes suspension cultured cells as experimental system...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28615721/spatio-temporal-variability-of-trace-elements-fingerprints-in-cockle-cerastoderma-edule-shells-and-its-relevance-for-tracing-geographic-origin
#10
Fernando Ricardo, Tânia Pimentel, Luciana Génio, Ricardo Calado
Understanding spatio-temporal variability of trace elements fingerprints (TEF) in bivalve shells is paramount to determine the discrimination power of this analytical approach and secure traceability along supply chains. Spatio-temporal variability of TEF was assessed in cockle (Cerastoderma edule) shells using inductively coupled plasma-mass spectrometry (ICP-MS). Four elemental ratios (Mg/Ca, Mn/Ca, Sr/Ca and Ba/Ca) were measured from the shells of specimens originating from eight different ecosystems along the Portuguese coast, as well as from four different areas, within one of them, over two consecutive years (2013 and 2014)...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28599967/challenging-surgical-dogma-in-the-management-of-proximal-esophageal-atresia-with-distal-tracheoesophageal-fistula-outcomes-from-the-midwest-pediatric-surgery-consortium
#11
Dave R Lal, Samir K Gadepalli, Cynthia D Downard, Daniel J Ostlie, Peter C Minneci, Ruth M Swedler, Thomas H Chelius, Laura Cassidy, Cooper T Rapp, Deborah Billmire, Steven Bruch, R Carland Burns, Katherine J Deans, Mary E Fallat, Jason D Fraser, Julia Grabowski, Ferdynand Hebel, Michael A Helmrath, Ronald B Hirschl, Rashmi Kabre, Jonathan Kohler, Matthew P Landman, Charles M Leys, Grace Z Mak, Jessica Raque, Beth Rymeski, Jacqueline M Saito, Shawn D St Peter, Daniel von Allmen, Brad W Warner, Thomas T Sato
PURPOSE: Perioperative management of infants with esophageal atresia and tracheoesophageal fistula (EA/TEF) is frequently based on surgeon experience and dogma rather than evidence-based guidelines. This study examines whether commonly perceived important aspects of practice affect outcome in a contemporary multi-institutional cohort of patients undergoing primary repair for the most common type of esophageal atresia anomaly, proximal EA with distal TEF. METHODS: The Midwest Pediatric Surgery Consortium conducted a multicenter, retrospective study examining selected outcomes on infants diagnosed with proximal EA with distal TEF who underwent primary repair over a 5-year period (2009-2014), with a minimum 1-year follow up, across 11 centers...
June 1, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28583493/endoscopic-esophageal-and-tracheal-cauterization-for-closure-of-recurrent-tracheoesophageal-fistula-a-case-report-and-review-of-the-literature
#12
REVIEW
Stacie Gregory, Robert H Chun, Daiva Parakininkas, Louella Amos, Roger Fons, Diana G Lerner, Dave R Lal, Cecille Sulman
OBJECTIVE: Recurrent tracheoesophageal fistula (TEF) can be a diagnostic and therapeutic challenge. Traditional treatment is thoracotomy, which carries significant morbidity and technical difficulty especially in a previously operated field. Recently, endoscopic techniques have been advocated as a primary approach for treatment of recurrent TEF prior to open repair. This case report describes the endoscopic technique used to address a recurrent TEF. The existing literature of all reported endoscopic cauterization methods is reviewed...
July 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28580843/risk-assessment-of-combined-exposure-to-alkenylbenzenes-through-consumption-of-plant-food-supplements-containing-parsley-and-dill
#13
Abdalmajeed M Alajlouni, Amer J Al-Malahmeh, Sebastiaan Wesseling, Marina Kalli, Jacques Vervoort, Ivonne M C M Rietjens
A risk assessment was performed of parsley and dill based plant food supplements (PFS) containing apiol and related alkenylbenzenes. First the levels of the alkenylbenzenes in the PFS and the resulting estimated daily intake (EDI) resulting from use of the PFS were quantified. Since most PFS appeared to contain more than one alkenylbenzene a combined risk assessment was performed based on equal potency or using a so-called Toxic Equivalency (TEQ) approach based on Toxic Equivalency Factors (TEFs) for the different alkenylbenzenes...
June 5, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28580312/the-role-of-radiofrequency-hyperthermia-in-the-radiosensitization-of-a-human-prostate-cancer-cell-line
#14
Azam Janati Esfahani, Seied Rabi Mahdavi, Mohammad Bagher Shiran, Samideh Khoei
OBJECTIVE: This study evaluated enhanced induced DNA damages and apoptosis of a spheroid culture of DU145 prostate cancer cells treated by a combination of radiofrequency hyperthermia (RF HT) with radiation treatment (RT) from an external radiotherapy machine compared to RT alone. MATERIALS AND METHODS: In this experimental study, DU145 cells were cultured as spheroids until they reached 300 µm in diameter. We exposed these cultures to either: RF HT for 90 minutes at 43˚C originated from a Celsius TCS system, RF HT followed by RT at doses of 2 Gy or 4 Gy (15 MV energy) with 15-minute interval, or RT alone at the above mentioned doses...
2017: Cell Journal
https://www.readbyqxmd.com/read/28580311/enhanced-dna-damages-of-human-prostate-cancer-cells-induced-by-radiofrequency-capacitive-hyperthermia-pre-and-post-x-rays-6-mv-versus-15-mv
#15
Seied Rabi Mahdavi, Azam Janati Esfahani, Mohammad Bagher Shiran, Samideh Khoei, Nader Estiri
OBJECTIVE: This study aimed to determine the effect of 13.56 MHz radiofrequency (RF) capacitive hyperthermia (HT) on radiosensivity of human prostate cancer cells pre and post X-ray radiation treatment (RT). MATERIALS AND METHODS: In this experimental study, the human prostate cancer cell line DU145 was cultured as 300 µm diameter spheroids. We divided the spheroids into group I: control, group II: HT at 43˚C for 30 minutes (HT), group III: 4 Gy irradiation with 6 MV X-ray [RT (6 MV)], group IV: 4 Gy irradiation with 15 MV X-ray [RT (15 MV)], group V: HT+RT (6 MV), group VI: HT+RT (15 MV), group VII: RT (6 MV)+HT, and group VIII: RT (15 MV)+HT...
2017: Cell Journal
https://www.readbyqxmd.com/read/28575191/recurrent-and-congenital-tracheoesophageal-fistula-in-adults%C3%A2
#16
Peter Downey, William Middlesworth, Matthew Bacchetta, Joshua Sonett
OBJECTIVES: Recurrent congenital tracheoesophageal fistula (TEF) is well documented in infancy but may also present later in life. This study reviews our experience with the clinical presentation, diagnosis and management of 5 recurrent and 2 primary congenital tracheoesophageal fistulas (TEF) in adult patients. There are no literature series of late recurrence of TEF (repaired in childhood and recurring in adulthood) and relatively few reported cases of initial adult presentation of TEF...
May 31, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28573516/-persistent-organic-contaminants-in-food-exposure-hazard-potential-and-health-assessment
#17
REVIEW
Ulrike Pabel, Thorsten Buhrke, Klaus Abraham, Thilo Nölke, Matthias Gehling, Alfonso Lampen, Monika Lahrssen-Wiederholt, Reiner Wittkowski
Environmental emissions of organic contaminants are caused by man-made and natural combustion processes, industrial production facilities, and the release from products. Food represents the main source of human exposure for some of these compounds. This is the case for three groups of persistent organic contaminants: (1) per- and polyfluoroalkyl substances (PFAS), (2) polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), polychlorinated biphenyls (PCBs), and for (3) polycyclic aromatic hydrocarbons (PAHs)...
June 1, 2017: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
https://www.readbyqxmd.com/read/28556924/determination-and-risk-assessment-of-naturally-occurring-genotoxic-and-carcinogenic-alkenylbenzenes-in-nutmeg-based-plant-food-supplements
#18
Amer J Al-Malahmeh, Abdalmajeed M Alajlouni, Jia Ning, Sebastiaan Wesseling, Jacques Vervoort, Ivonne M C M Rietjens
A risk assessment of nutmeg-based plant food supplements (PFS) containing different alkenylbenzenes was performed based on the alkenylbenzene levels quantified in a series of PFS collected via the online market. The estimated daily intake (EDI) of the alkenylbenzenes amounted to 0.3 to 312 μg kg(-1) body weight (bw) for individual alkenylbenzenes, to 1.5 to 631 μg kg(-1) bw when adding up the alkenylbenzene levels assuming equal potency, and to 0.4 to 295 μg kg(-1) bw when expressed in safrole equivalents using toxic equivalency factors (TEFs)...
May 29, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28554817/outcome-of-primary-repair-in-extremely-and-very-low-birth-weight-infants-with-esophageal-atresia-distal-tracheoesophageal-fistula
#19
Andreas Schmidt, Florian Obermayr, Justus Lieber, Christian Gille, Frank Fideler, Joerg Fuchs
PURPOSE: The optimal surgical management of extremely (ELBW) and very low-birth-weight (VLBW) neonates with esophageal atresia and distal tracheoesophageal fistula (EA/TEF) (Gross type C) is still debated. The aim of this study was to evaluate the surgical outcome of primary repair in these patients and compare it to ≥1500g neonates. METHODS: Medical records of neonates with repaired EA from 2002 to 2016 were reviewed. RESULTS: 4 ELBW, 7 VLBW, and 24 ≥1500g infants had type C EA/TEF and underwent primary repair...
May 17, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28552310/separate-screening-of-brominated-and-chlorinated-dioxins-in-field-samples-using-in%C3%A2-vitro-reporter-gene-assays-with-rat-and-mouse-hepatoma-cell-lines
#20
Go Suzuki, Masafumi Nakamura, Chieko Michinaka, Nguyen Minh Tue, Hiroshi Handa, Hidetaka Takigami
In 2011, a joint World Health Organization (WHO) and United Nations Environment Programme expert panel recommended the use of the WHO toxicity equivalency factor (TEF) scheme for assessment of the human health risks of brominated dioxins which have different sources from chlorinated dioxins. Therefore, WHO toxic equivalents (TEQs) should be determined for both chlorinated and brominated dioxins. As alternative to the conventional but time-consuming dioxin analysis using gas chromatography high-resolution mass spectrometry, we have developed a screening method involving cleanup and separation of brominated dioxins from chlorinated dioxins in field samples and subsequent analysis by CALUX (chemically activated luciferase expression) assays which are in vitro reporter gene assays detecting dioxin-like compounds...
July 4, 2017: Analytica Chimica Acta
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