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https://www.readbyqxmd.com/read/27896423/long-term-outcomes-following%C3%A2-h-type-tracheoesophageal-fistula%C3%A2-repair-in-infants
#1
Augusto Zani, Luai Jamal, Giovanni Cobellis, Justyna M Wolinska, Samuel Fung, Evan J Propst, Priscilla P L Chiu, Agostino Pierro
PURPOSE: To evaluate outcomes following repair of H-type tracheoesophageal fistula (TEF). METHODS: Retrospective chart review of infants with H-type TEF treated at our institution between 2000 and 2014. Patient demographics, surgical management, and postoperative function were evaluated. RESULTS: Of the 268 patients with esophageal atresia/TEF treated at our center, 16 (6%) had an H-type TEF (10 males). Thirteen (81%) had associated anomalies...
November 28, 2016: Pediatric Surgery International
https://www.readbyqxmd.com/read/27896158/congenital-duodenal-obstruction-in-neonates-over-13-years-experience-from-a-single-centre
#2
Parveen Kumar, Chiranjiv Kumar, Prince Raj Pandey, Yogesh Kumar Sarin
AIM: To study the prevalence of associated anomalies with neonatal duodenal obstruction and factors impacting short-term survival. MATERIAL AND METHODS: Records of 31 neonates with neonatal duodenal obstruction could be retrieved and analyzed for a 13.5-year-period (October 2003-May 2016). M:F ratio was 1.58:1. The mean birth weight was 2.15 kg; 12 patients were preterm. Etiologies included duodenal atresia (n=23), duodenal web (n=8) and malrotation of gut (n= 6)...
October 2016: Journal of Neonatal Surgery
https://www.readbyqxmd.com/read/27875095/routine-bronchoscopy-and-fogarty-catheter-occlusion-of-tracheoesophageal-fistulas
#3
Victoria K Pepper, Laura A Boomer, Arlyne K Thung, Jonathan M Grischkan, Karen A Diefenbach
INTRODUCTION: Use of a Fogarty catheter for emergent occlusion of tracheoesophageal fistulas (TEFs) has been described for use in unstable neonates. Our purpose was to describe a case series of elective Fogarty catheter occlusion of the TEF. MATERIALS AND METHODS: A formal operative laryngoscopy and rigid bronchoscopy were performed with Fogarty catheter placement into the fistula before surgical intervention. The balloon was inflated and gentle traction provided occlusion of the tract...
November 22, 2016: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/27862796/proton-gated-photoisomerization-of-amino-substituted-dibenzofulvene-rotors
#4
Udaya Jayasundara, HyunJong Kim, Krishna Sahteli, Joseph I Cline, Thomas W Bell
Derivatives of 9-(2,2,2-triphenylethylidene)-fluorene (TEF) undergo E/Z photoisomerization and are candidates for light-powered molecular actuators and switches. The 2-substituted derivatives bearing an amino group (ATEF) or a dimethylamino group (DTEF) are weakly photoactive in the absence of acids, but protonation increases photoisomerization quantum yields by factors of 30-60. Such compounds may be useful for incorporation into pH-switchable photoactive devices.
November 14, 2016: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/27858521/preliminary-study-of-video-based-pediatric-endoscopic-surgical-skill-assessment-using-a-neonatal-esophageal-atresia-tracheoesophageal-fistula-model
#5
Kyoichi Deie, Tetsuya Ishimaru, Shinya Takazawa, Kanako Harada, Naohiko Sugita, Mamoru Mitsuishi, Jun Fujishiro, Tadashi Iwanaka
PURPOSE: Thoracoscopic esophageal atresia/tracheoesophageal fistula (EA/TEF) repair in neonates is technically difficult because of the small working space and fragility of tissues. This study aimed to show the construct validity of the neonatal EA/TEF simulator by video-based assessment of endoscopic suturing skill. MATERIALS AND METHODS: A rapid-prototyped neonatal chest model with an artificial esophagus model similar to the actual neonatal esophagus was developed...
November 18, 2016: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/27827901/structure-activity-relationship-studies-using-natural-and-synthetic-okadaic-acid-dinophysistoxin-toxins
#6
Michael J Twiner, Gregory J Doucette, Yucheng Pang, Chao Fang, Craig J Forsyth, Christopher O Miles
Okadaic acid (OA) and the closely related dinophysistoxins (DTXs) are algal toxins that accumulate in shellfish and are known serine/threonine protein phosphatase (ser/thr PP) inhibitors. Phosphatases are important modulators of enzyme activity and cell signaling pathways. However, the interactions between the OA/DTX toxins and phosphatases are not fully understood. This study sought to identify phosphatase targets and characterize their structure-activity relationships (SAR) with these algal toxins using a combination of phosphatase activity and cytotoxicity assays...
November 4, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27825338/sequential-and-counter-selectable-cassettes-for-fission-yeast
#7
Hanna Amelina, Vera Moiseeva, Laura Catharine Collopy, Siân Rosanna Pearson, Christine Anne Armstrong, Kazunori Tomita
BACKGROUND: Fission yeast is one of the most commonly used model organisms for studying genetics. For selection of desirable genotypes, antibiotic resistance cassettes are widely integrated into the genome near genes of interest. In yeasts, this is achieved by PCR amplification of the cassette flanked by short homology sequences, which can be incorporated by homology directed repair. However, the currently available cassettes all share the same tef promoter and terminator sequences. It can therefore be challenging to perform multiple genetic modifications by PCR-based targeting, as existing resistance cassettes in strains can be favored for recombination due to shared homology between the cassettes...
November 8, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27790070/first-report-of-leaf-spot-caused-by-alternaria-tenuissima-on-black-chokeberry-aronia-melanocarpa-in-korea
#8
Jung-In Wee, Jong-Han Park, Chang-Gi Back, Young-Hyun You, Taehyun Chang
In July 2015, diseased leaves of black chokeberry (Aronia melanocarpa) were observed in Danyang and Gochang, Korea. The symptoms appeared as circular or irregular brown leaf spots, from which Alternaria tenuissima was isolated. The isolates were cultured on potato dextrose agar, and their morphological characteristics were observed under a light microscope. The colonies were whitish to ash colored. The pathogenicity test on healthy black chokeberry leaves produced circular brown spots, in line with the original symptoms...
September 2016: Mycobiology
https://www.readbyqxmd.com/read/27785539/computed-tomography-and-upper-gastrointestinal-series-findings-of-esophageal-bronchi-in-infants
#9
Gabrielle C Colleran, Ciara E Ryan, Edward Y Lee, Brian Sweeney, David Rea, Clare Brenner
BACKGROUND: Esophageal bronchus is a rare form of communicating bronchopulmonary foregut malformation and a rare but important cause of an opaque hemithorax on chest radiography. A higher incidence of esophageal bronchus is associated with esophageal atresia, tracheo-esophageal fistula (TEF) and VACTERL (vertebral defects, anal atresia, cardiac defects, tracheo-esophageal fistula, renal anomalies, and limb abnormalities) association. In the presence of these conditions, the pediatric radiologist may be the first to consider the diagnosis of esophageal bronchus or esophageal lung...
October 26, 2016: Pediatric Radiology
https://www.readbyqxmd.com/read/27779122/track-average-let-of-secondary-electrons-generated-in-lif-mg-ti-and-liquid-water-by-20-300%C3%A2-kv-x-ray-137-cs-and-60-co-beams
#10
A Cabrera-Santiago, G Massillon-Jl
Electrons generated in matter by photons could be a fundamental basis for an adequate analysis of radiation effects and damage. We have studied separately the 'primary electrons' generated directly by photons from the 'secondary electrons' (SE) produced by electron-electron interactions. In this work, track-average linear energy transfer, [Formula: see text], of SE in LiF:Mg,Ti and liquid water produced by twelve photon energy beams from 20 kV x-ray to (60)Co gamma rays have been investigated using the EGSnrc Monte Carlo Code...
November 21, 2016: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/27775206/an-improved-method-for-calculating-toxicity-based-pollutant-loads-part-2-application-to-contaminants-discharged-to-the-great-barrier-reef-queensland-australia
#11
Rachael A Smith, Michael St J Warne, Kerrie Mengersen, Ryan D R Turner
Pollutant loads are widely used to set pollution reduction targets and assess regulatory compliance for the protection of receiving waterbodies. However, when a pollutant load is comprised of a mixture of chemicals, reducing the overall load (mass) will not necessarily reduce the toxicity by a similar amount. This can be overcome by setting targets based on toxicity-based loads (toxic loads), where the load is modified according to the relative toxicity (expressed as toxic equivalency factors- TEFs) of each toxicant...
October 24, 2016: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/27771948/level-of-alkenylbenzenes-in-parsley-and-dill-based-teas-and-associated-risk-assessment-using-the-margin-of-exposure-approach
#12
Abdalmajeed M Alajlouni, Amer J Almalahmeh, Farida Nur Isnaeni, Sebastiaan Wesseling, Jacques Vervoort, Ivonne Magdalena Catharina Maria Rietjens
Risk assessment of parsley and dill based teas that contain alkenylbenzenes was performed. To this end the estimated daily intake (EDI) of alkenylbenzenes resulting from use of the teas was quantified. Since most teas appeared to contain more than one alkenylbenzene a combined risk assessment was performed based on equal potency of all alkenylbenzenes or using a so-called Toxic Equivalency (TEQ) approach through defining Toxic Equivalency Factors (TEFs) for the different alkenylbenzenes. The EDI values resulting from consuming one cup of tea a day were 0...
October 22, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27766254/increased-cost-of-motor-activity-and-heat-transfer-between-non-shivering-thermogenesis-motor-activity-and-thermic-effect-of-feeding-in-mice-housed-at-room-temperature-implications-in-pre-clinical-studies
#13
Patrick C Even, Anne Blais
The components of energy expenditure, total metabolic rate (TMR), resting metabolic rate (RMR), thermogenic response to feeding (TEF), activity, and cost of activity were measured in fed and fasted mice housed at 22 and 30°C. Mice housed at 22°C had more than two times larger TMR and RMR. Mice at 22°C were less active when fasted but more active when fed. Cost of activity was nearly doubled in the fasted and in the fed state. Analysis of the short-term relation between TMR, RMR, and bouts of activity showed that, at 22°C, the bouts of activity induced a decrease in the intensity of RMR that reflected the reduced need for thermal regulation induced by the heat released from muscular contraction...
2016: Frontiers in Nutrition
https://www.readbyqxmd.com/read/27764137/role-of-teffector-memory-cells-tbx21-gene-expression-and-t-cell-homing-receptor-on-type-1-reaction-in-borderline-lepromatous-leprosy-patients
#14
Luciana Nahar Dos Santos, Pedro Henrique Lopes da Silva, Iris Maria Peixoto Alvim, José Augusto da Costa Nery, Flávio Alves Lara, Euzenir Nunes Sarno, Danuza Esquenazi
In spite of hyporesponsivity to Mycobacterium leprae, borderline lepromatous (BL) patients show clinical and immunological instability, and undergo frequent acute inflammatory episodes such as type 1 reaction (T1R), which may cause nerve damages. This work focused on the participation of T cell subsets from blood and skin at T1R onset. We observed a significantly increased ex vivo frequency of both effector and memory CD4+ and CD8+ T cells in T1R group. Besides, ex vivo frequency of T cell homing receptor, the Cutaneous Leukocyte-associated Antigen (CLA) was significantly increased in T cells from T1R patients...
2016: PloS One
https://www.readbyqxmd.com/read/27761449/tracheoesophageal-fistula-induced-by-invasive-pulmonary-aspergillosis
#15
Yuetian Yu, Cheng Zhu, Xiaozhe Qian, Yuan Gao
Invasive pulmonary aspergillosis (IPA) is commonly seen in immunocompromised patients, and tracheoesophageal fistula (TEF) induced by IPA is rare and seldom reported. Management of these critically ill patients is challenging and often requires a multidisciplinary approach. The authors reported an adult suffering from aplastic anemia who developed TEF caused by IPA. The diagnosis was confirmed following bronchoscopy and histopathological examination. Antifungal and bronchoscopic intervention provided a cure without any recurrence as yet...
September 2016: Annals of Translational Medicine
https://www.readbyqxmd.com/read/27743466/an-improved-method-for-calculating-toxicity-based-pollutant-loads-part-1-method-development
#16
Rachael A Smith, Michael St J Warne, Kerrie Mengersen, Ryan D R Turner
Pollutant loads are a means for assessing regulatory compliance and setting targets to reduce pollution entering receiving waterbodies. However, a pollutant load is often comprised of multiple chemicals, which may exert joint toxicity on biota. When the ultimate goal for assessing pollutant loads is to protect ecosystems from adverse effects of toxicants, then the total pollutant load needs to be calculated based on the principles of mixture toxicology. In this paper, an improved method is proposed to convert a pollutant load to a toxicity-based load (toxic load) using a modified toxic equivalency factor (TEF) derivation method...
October 15, 2016: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/27721177/novel-quercetin-derivative-tef-induces-er-stress-and-mitochondria-mediated-apoptosis-in-human-colon-cancer-hct-116-cells
#17
Imran Khan, Souren Paul, Rekha Jakhar, Monika Bhardwaj, Jaehong Han, Sun Chul Kang
Although quercetin is very well known for its anticancer activity, however it shows some drawbacks. Herein, we have evaluated the apoptotic effect TEF (5, 3'-dihydroxy-3, 7, 4'-triethoxyflavone), a newly synthesized quercetin derivative on HCT-116 colon cancer cells. After 24h of treatment, the proliferation of colon cancer cells was inhibited by TEF. TEF induced apoptosis, as confirmed by the presence of fragmented nuclei, reduced mitochondrial membrane potential, and elevated cytoplasmic and mitochondrial reactive oxygen species (ROS) levels...
October 6, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27706736/morphological-and-molecular-characterization-of-cladosporium-cladosporioides-species-complex-causing-pecan-tree-leaf-spot
#18
C Walker, M F B Muniz, J M Rolim, R R O Martins, V C Rosenthal, C G Maciel, R Mezzomo, L R S Reiniger
The objective of this study was to characterize species of the Cladosporium cladosporioides complex isolated from pecan trees (Carya illinoinensis) with symptoms of leaf spot, based on morphological and molecular approaches. Morphological attributes were assessed using monosporic cultures on potato dextrose agar medium, which were examined for mycelial growth, sporulation, color, and conidia and ramoconidia size. Molecular characterization comprised isolation of DNA and subsequent amplification of the translation elongation factor 1α (TEF-1α) region...
September 16, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27637489/new-families-of-single-integration-vectors-and-gene-tagging-plasmids-for-genetic-manipulations-in-budding-yeast
#19
Victoria Wosika, Eric Durandau, Clémence Varidel, Delphine Aymoz, Marta Schmitt, Serge Pelet
The tractability of the budding yeast genome has provided many insights into the fundamental mechanisms regulating cellular life. With the advent of synthetic biology and single-cell measurements, novel tools are required to manipulate the yeast genome in a more controlled manner. We present, here, a new family of yeast shuttle vectors called single integration vectors (pSIV). Upon transformation in yeast, these plasmids replace the entire deficient auxotrophy marker locus by a cassette containing an exogenous marker...
September 16, 2016: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/27616617/categorization-and-repair-of-recurrent-and-acquired-tracheoesophageal-fistulae-occurring-after-esophageal-atresia-repair
#20
C Jason Smithers, Thomas E Hamilton, Michael A Manfredi, Lawrence Rhein, Peter Ngo, Dorothy Gallagher, John E Foker, Russell W Jennings
PURPOSE: Recurrent trachea-esophageal fistula (recTEF) is a frequent (5%-10%) complication of congenital TEF (conTEF) and esophageal atresia (EA) repair. In addition, postoperative acquired TEF (acqTEF) can occur in addition to or even in the absence of prior conTEF in the setting of esophageal anastomotic complications. Reliable repair often proves difficult by endoluminal or standard surgical techniques. We present the results of an approach that reliably identifies the TEF and facilitates airway closure as well as repair of associated tracheal and esophageal problems...
August 31, 2016: Journal of Pediatric Surgery
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