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https://www.readbyqxmd.com/read/28623582/analysis-of-in-vivo-chromatin-and-protein-interactions-of-arabidopsis-transcript-elongation-factors
#1
Alexander Pfab, Wojciech Antosz, Philipp Holzinger, Astrid Bruckmann, Joachim Griesenbeck, Klaus D Grasser
A central step to elucidate the function of proteins commonly comprises the analysis of their molecular interactions in vivo. For nuclear regulatory proteins this involves determining protein-protein interactions as well as mapping of chromatin binding sites. Here, we present two protocols to identify protein-protein and chromatin interactions of transcript elongation factors (TEFs) in Arabidopsis. The first protocol (Subheading 3.1) describes protein affinity-purification coupled to mass spectrometry (AP-MS) that utilizes suspension cultured cells as experimental system...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28615721/spatio-temporal-variability-of-trace-elements-fingerprints-in-cockle-cerastoderma-edule-shells-and-its-relevance-for-tracing-geographic-origin
#2
Fernando Ricardo, Tânia Pimentel, Luciana Génio, Ricardo Calado
Understanding spatio-temporal variability of trace elements fingerprints (TEF) in bivalve shells is paramount to determine the discrimination power of this analytical approach and secure traceability along supply chains. Spatio-temporal variability of TEF was assessed in cockle (Cerastoderma edule) shells using inductively coupled plasma-mass spectrometry (ICP-MS). Four elemental ratios (Mg/Ca, Mn/Ca, Sr/Ca and Ba/Ca) were measured from the shells of specimens originating from eight different ecosystems along the Portuguese coast, as well as from four different areas, within one of them, over two consecutive years (2013 and 2014)...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28599967/challenging-surgical-dogma-in-the-management-of-proximal-esophageal-atresia-with-distal-tracheoesophageal-fistula-outcomes-from-the-midwest-pediatric-surgery-consortium
#3
Dave R Lal, Samir K Gadepalli, Cynthia D Downard, Daniel J Ostlie, Peter C Minneci, Ruth M Swedler, Thomas H Chelius, Laura Cassidy, Cooper T Rapp, Deborah Billmire, Steven Bruch, R Carland Burns, Katherine J Deans, Mary E Fallat, Jason D Fraser, Julia Grabowski, Ferdynand Hebel, Michael A Helmrath, Ronald B Hirschl, Rashmi Kabre, Jonathan Kohler, Matthew P Landman, Charles M Leys, Grace Z Mak, Jessica Raque, Beth Rymeski, Jacqueline M Saito, Shawn D St Peter, Daniel von Allmen, Brad W Warner, Thomas T Sato
PURPOSE: Perioperative management of infants with esophageal atresia and tracheoesophageal fistula (EA/TEF) is frequently based on surgeon experience and dogma rather than evidence-based guidelines. This study examines whether commonly perceived important aspects of practice affect outcome in a contemporary multi-institutional cohort of patients undergoing primary repair for the most common type of esophageal atresia anomaly, proximal EA with distal TEF. METHODS: The Midwest Pediatric Surgery Consortium conducted a multicenter, retrospective study examining selected outcomes on infants diagnosed with proximal EA with distal TEF who underwent primary repair over a 5-year period (2009-2014), with a minimum 1-year follow up, across 11 centers...
June 1, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28583493/endoscopic-esophageal-and-tracheal-cauterization-for-closure-of-recurrent-tracheoesophageal-fistula-a-case-report-and-review-of-the-literature
#4
REVIEW
Stacie Gregory, Robert H Chun, Daiva Parakininkas, Louella Amos, Roger Fons, Diana G Lerner, Dave R Lal, Cecille Sulman
OBJECTIVE: Recurrent tracheoesophageal fistula (TEF) can be a diagnostic and therapeutic challenge. Traditional treatment is thoracotomy, which carries significant morbidity and technical difficulty especially in a previously operated field. Recently, endoscopic techniques have been advocated as a primary approach for treatment of recurrent TEF prior to open repair. This case report describes the endoscopic technique used to address a recurrent TEF. The existing literature of all reported endoscopic cauterization methods is reviewed...
July 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28580843/risk-assessment-of-combined-exposure-to-alkenylbenzenes-through-consumption-of-plant-food-supplements-containing-parsley-and-dill
#5
Abdalmajeed M Alajlouni, Amer J Al-Malahmeh, Sebastiaan Wesseling, Marina Kalli, Jacques Vervoort, Ivonne M C M Rietjens
A risk assessment was performed of parsley and dill based plant food supplements (PFS) containing apiol and related alkenylbenzenes. First the levels of the alkenylbenzenes in the PFS and the resulting estimated daily intake (EDI) resulting from use of the PFS were quantified. Since most PFS appeared to contain more than one alkenylbenzene a combined risk assessment was performed based on equal potency or using a so-called Toxic Equivalency (TEQ) approach based on Toxic Equivalency Factors (TEFs) for the different alkenylbenzenes...
June 5, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28580312/the-role-of-radiofrequency-hyperthermia-in-the-radiosensitization-of-a-human-prostate-cancer-cell-line
#6
Azam Janati Esfahani, Seied Rabi Mahdavi, Mohammad Bagher Shiran, Samideh Khoei
OBJECTIVE: This study evaluated enhanced induced DNA damages and apoptosis of a spheroid culture of DU145 prostate cancer cells treated by a combination of radiofrequency hyperthermia (RF HT) with radiation treatment (RT) from an external radiotherapy machine compared to RT alone. MATERIALS AND METHODS: In this experimental study, DU145 cells were cultured as spheroids until they reached 300 µm in diameter. We exposed these cultures to either: RF HT for 90 minutes at 43˚C originated from a Celsius TCS system, RF HT followed by RT at doses of 2 Gy or 4 Gy (15 MV energy) with 15-minute interval, or RT alone at the above mentioned doses...
2017: Cell Journal
https://www.readbyqxmd.com/read/28580311/enhanced-dna-damages-of-human-prostate-cancer-cells-induced-by-radiofrequency-capacitive-hyperthermia-pre-and-post-x-rays-6-mv-versus-15-mv
#7
Seied Rabi Mahdavi, Azam Janati Esfahani, Mohammad Bagher Shiran, Samideh Khoei, Nader Estiri
OBJECTIVE: This study aimed to determine the effect of 13.56 MHz radiofrequency (RF) capacitive hyperthermia (HT) on radiosensivity of human prostate cancer cells pre and post X-ray radiation treatment (RT). MATERIALS AND METHODS: In this experimental study, the human prostate cancer cell line DU145 was cultured as 300 µm diameter spheroids. We divided the spheroids into group I: control, group II: HT at 43˚C for 30 minutes (HT), group III: 4 Gy irradiation with 6 MV X-ray [RT (6 MV)], group IV: 4 Gy irradiation with 15 MV X-ray [RT (15 MV)], group V: HT+RT (6 MV), group VI: HT+RT (15 MV), group VII: RT (6 MV)+HT, and group VIII: RT (15 MV)+HT...
2017: Cell Journal
https://www.readbyqxmd.com/read/28575191/recurrent-and-congenital-tracheoesophageal-fistula-in-adults%C3%A2
#8
Peter Downey, William Middlesworth, Matthew Bacchetta, Joshua Sonett
OBJECTIVES: Recurrent congenital tracheoesophageal fistula (TEF) is well documented in infancy but may also present later in life. This study reviews our experience with the clinical presentation, diagnosis and management of 5 recurrent and 2 primary congenital tracheoesophageal fistulas (TEF) in adult patients. There are no literature series of late recurrence of TEF (repaired in childhood and recurring in adulthood) and relatively few reported cases of initial adult presentation of TEF...
May 31, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28573516/-persistent-organic-contaminants-in-food-exposure-hazard-potential-and-health-assessment
#9
REVIEW
Ulrike Pabel, Thorsten Buhrke, Klaus Abraham, Thilo Nölke, Matthias Gehling, Alfonso Lampen, Monika Lahrssen-Wiederholt, Reiner Wittkowski
Environmental emissions of organic contaminants are caused by man-made and natural combustion processes, industrial production facilities, and the release from products. Food represents the main source of human exposure for some of these compounds. This is the case for three groups of persistent organic contaminants: (1) per- and polyfluoroalkyl substances (PFAS), (2) polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), polychlorinated biphenyls (PCBs), and for (3) polycyclic aromatic hydrocarbons (PAHs)...
June 1, 2017: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
https://www.readbyqxmd.com/read/28556924/determination-and-risk-assessment-of-naturally-occurring-genotoxic-and-carcinogenic-alkenylbenzenes-in-nutmeg-based-plant-food-supplements
#10
Amer J Al-Malahmeh, Abdalmajeed M Alajlouni, Jia Ning, Sebastiaan Wesseling, Jacques Vervoort, Ivonne M C M Rietjens
A risk assessment of nutmeg-based plant food supplements (PFS) containing different alkenylbenzenes was performed based on the alkenylbenzene levels quantified in a series of PFS collected via the online market. The estimated daily intake (EDI) of the alkenylbenzenes amounted to 0.3 to 312 μg kg(-1) body weight (bw) for individual alkenylbenzenes, to 1.5 to 631 μg kg(-1) bw when adding up the alkenylbenzene levels assuming equal potency, and to 0.4 to 295 μg kg(-1) bw when expressed in safrole equivalents using toxic equivalency factors (TEFs)...
May 29, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28554817/outcome-of-primary-repair-in-extremely-and-very-low-birth-weight-infants-with-esophageal-atresia-distal-tracheoesophageal-fistula
#11
Andreas Schmidt, Florian Obermayr, Justus Lieber, Christian Gille, Frank Fideler, Joerg Fuchs
PURPOSE: The optimal surgical management of extremely (ELBW) and very low-birth-weight (VLBW) neonates with esophageal atresia and distal tracheoesophageal fistula (EA/TEF) (Gross type C) is still debated. The aim of this study was to evaluate the surgical outcome of primary repair in these patients and compare it to ≥1500g neonates. METHODS: Medical records of neonates with repaired EA from 2002 to 2016 were reviewed. RESULTS: 4 ELBW, 7 VLBW, and 24 ≥1500g infants had type C EA/TEF and underwent primary repair...
May 17, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28552310/separate-screening-of-brominated-and-chlorinated-dioxins-in-field-samples-using-in%C3%A2-vitro-reporter-gene-assays-with-rat-and-mouse-hepatoma-cell-lines
#12
Go Suzuki, Masafumi Nakamura, Chieko Michinaka, Nguyen Minh Tue, Hiroshi Handa, Hidetaka Takigami
In 2011, a joint World Health Organization (WHO) and United Nations Environment Programme expert panel recommended the use of the WHO toxicity equivalency factor (TEF) scheme for assessment of the human health risks of brominated dioxins which have different sources from chlorinated dioxins. Therefore, WHO toxic equivalents (TEQs) should be determined for both chlorinated and brominated dioxins. As alternative to the conventional but time-consuming dioxin analysis using gas chromatography high-resolution mass spectrometry, we have developed a screening method involving cleanup and separation of brominated dioxins from chlorinated dioxins in field samples and subsequent analysis by CALUX (chemically activated luciferase expression) assays which are in vitro reporter gene assays detecting dioxin-like compounds...
July 4, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28545763/13-ribs-as-a-predictor-of-long-gap-esophageal-atresia-myth-or-reality-analysis-of-associated-findings-of-esophageal-atresia-and-abnormal-rib-count
#13
Jonathan Durell, Haitham Dagash, Bala Eradi, Ashok Rajimwale, Shawqui Nour, Nitin Patwardhan
BACKGROUND: The presence of 13 pairs of ribs on pre-operative chest x-ray has been postulated to be an indicator for long gap esophageal atresia (EA). This study sought to determine the validity of this theory and identify associated pathological conditions in patients with EA and abnormal rib count. METHODS: Babies with EA from January 2005 - December 2012 were retrospectively analyzed. Information was gathered from neonatal health records and operation notes. Chest x-rays were reviewed to determine rib count...
May 2, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28533986/atorvastatin-alters-the-expression-of-genes-related-to-bile-acid-metabolism-and-circadian-clock-in-livers-of-mice
#14
Wen-Kai Li, Huan Li, Yuan-Fu Lu, Ying-Ying Li, Zidong Donna Fu, Jie Liu
AIM: Atorvastatin is a HMG-CoA reductase inhibitor used for hyperlipidemia. Atorvastatin is generally safe but may induce cholestasis. The present study aimed to examine the effects of atorvastatin on hepatic gene expression related to bile acid metabolism and homeostasis, as well as the expression of circadian clock genes in livers of mice. METHODS: Adult male mice were given atorvastatin (10, 30, and 100 mg/kg, po) daily for 30 days, and blood biochemistry, histopathology, and gene expression were examined...
2017: PeerJ
https://www.readbyqxmd.com/read/28533816/conferring-cellulose-degrading-ability-to-yarrowia-lipolytica-to-facilitate-a-consolidated-bioprocessing-approach
#15
Zhong-Peng Guo, Sophie Duquesne, Sophie Bozonnet, Gianluca Cioci, Jean-Marc Nicaud, Alain Marty, Michael Joseph O'Donohue
BACKGROUND: Yarrowia lipolytica, one of the most widely studied "nonconventional" oleaginous yeast species, is unable to grow on cellulose. Recently, we identified and overexpressed two endogenous β-glucosidases in Y. lipolytica, thus enabling this yeast to use cello-oligosaccharides as a carbon source for growth. Using this engineered yeast platform, we have now gone further toward building a fully cellulolytic Y. lipolytica for use in consolidated bioprocessing of cellulose. RESULTS: Initially, different essential enzyme components of a cellulase cocktail (i...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28532148/gluten-free-sources-of-fermentable-extract-effect-of-temperature-and-germination-time-on-quality-attributes-of-teff-eragrostis-tef-zucc-trotter-malt-and-wort
#16
Lidia Di Ghionno, Ombretta Marconi, Eung Gwan Lee, Christopher J Rice, Valeria Sileoni, Giuseppe Perretti
This study was conducted to evaluate the behavior of a white teff variety called Witkop during malting by using different parameters (germination temperature and duration) and to identify the best malting program. Samples were evaluated for standard quality malt and wort attributes, pasting characteristics, β-glucan and arabinoxylan content, and sugar profile. It was concluded that malting teff at 24 °C for 6 days produced acceptable malt in terms of quality attributes and sugar profile for brewing. The main attributes were 80...
June 1, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28528013/congenital-h-type-tracheoesophageal-fistula-a-multicenter-review-of-outcomes-in-a-rare-disease
#17
Sara C Fallon, Jacob C Langer, Shawn D St Peter, KuoJen Tsao, Caroline M Kellagher, Dave R Lal, Jill S Whitehouse, Diana L Diesen, Michael D Rollins, Elizabeth Pontarelli, Marcus M Malek, Corey W Iqbal, Jeffrey S Upperman, Charles M Leys, Mark L Wulkan, Sarah J Hill, Martin L Blakely, Timothy D Kane, David E Wesson
OBJECTIVE: To perform a multicenter review of outcomes in patients with H-type tracheoesophageal fistula (TEF) in order to better understand the incidence and causes of post-operative complications. BACKGROUND: H-type TEF without esophageal atresia (EA) is a rare anomaly with a fundamentally different management algorithm than the more common types of EA/TEF. Outcomes after surgical treatment of H-type TEF are largely unknown, but many authoritative textbooks describe a high incidence of respiratory complications...
May 11, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28481261/attenuating-the-biologic-drive-for-weight-regain-following-weight-loss-must-what-goes-down-always-go-back-up
#18
REVIEW
Christopher L Melby, Hunter L Paris, Rebecca M Foright, James Peth
Metabolic adaptations occur with weight loss that result in increased hunger with discordant simultaneous reductions in energy requirements-producing the so-called energy gap in which more energy is desired than is required. The increased hunger is associated with elevation of the orexigenic hormone ghrelin and decrements in anorexigenic hormones. The lower total daily energy expenditure with diet-induced weight loss results from (1) a disproportionately greater decrease in circulating leptin and resting metabolic rate (RMR) than would be predicted based on the decline in body mass, (2) decreased thermic effect of food (TEF), and (3) increased energy efficiency at work intensities characteristic of activities of daily living...
May 6, 2017: Nutrients
https://www.readbyqxmd.com/read/28457467/isolation-and-characterisation-of-nanoparticles-from-tef-and-maize-starch-modified-with-stearic-acid
#19
Wokadala O Cuthbert, Suprakas S Ray, Naushad M Emmambux
Nanoparticles were isolated from tef and maize starch modified with added stearic acid after pasting at 90°C for 130min. This was followed by thermo-stable alpha-amylase hydrolysis of the paste. The resultant residues were then characterized using X-ray diffraction (XRD), differential scanning calorimetry (DSC), dynamic laser scattering particle size distribution (DLPSD), atomic force microscopy (AFM) and high-resolution transmission electron microscopy (HRTEM). XRD and DSC showed that the isolated residues consisted of amylose-lipid complexes...
July 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28425876/characterization-of-the-translation-elongation-factor-1-%C3%AE-gene-in-a-wide-range-of-pathogenic-aspergillus-species
#20
Sadegh Nouripour-Sisakht, Bahram Ahmadi, Koichi Makimura, Sybren de Hoog, Yoshiko Umeda, Mohamed Mahdi Alshahni, Hossein Mirhendi
PURPOSE: We aimed to evaluate the resolving power of the translation elongation factor (TEF)-1α gene for phylogenetic analysis of Aspergillus species. METHODOLOGY: Sequences of 526 bp representing the coding region of the TEF-1α gene were used for the assessment of levels of intra- and inter-specific nucleotide polymorphism in 33 species of Aspergillus, including 57 reference, clinical and environmental strains. RESULTS: Analysis of TEF-1α sequences indicated a mean similarity of 92...
April 21, 2017: Journal of Medical Microbiology
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