keyword
MENU ▼
Read by QxMD icon Read
search

growth parameters

keyword
https://www.readbyqxmd.com/read/28346794/comparative-study-of-xenobiotic-free-media-for-the-cultivation-of-human-limbal-epithelial-stem-progenitor-cells
#1
Sheyla González, Luxia Chen, Sophie X Deng
The culture of human limbal epithelial stem/progenitor cells (LSCs) in the presence of animal components poses the risk of cross-species contamination in clinical applications. We quantitatively compared different xenobiotic-free culture media for the cultivation of human LSCs. LSCs were cultured from 2 × 2 mm limbal tissue explants on denuded human amniotic membrane with different xenobiotic-free culture media: CnT-Prime (CnT-PR) supplemented with 0%, 1%, 5%, and 10% human serum (HS), embryonic stem cell medium (ESCM) alone or in combination with the standard supplemented hormonal epithelium medium (SHEM, control) at a 1:1 dilution ratio, and modified SHEM (mSHEM), in which cholera toxin and dimethyl sulfoxide (DMSO) were removed, isoproterenol was added, and the epidermal growth factor concentration was reduced...
March 24, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28346107/a-high-throughput-method-for-measuring-drug-residence-time-using-the-transcreener-adp-assay
#2
Meera Kumar, Robert G Lowery
Analysis of drug-target residence times during drug development can result in improved efficacy, increased therapeutic window, and reduced side effects. Residence time can be estimated as the reciprocal of the dissociation rate ( koff) of an inhibitor from its target. The traditional methods for measuring koff require synthesis of labeled ligands or low-throughput label-free methods. To provide an alternative that is better suited to an automated high-throughput screening (HTS) environment, we adapted a classic "jump dilution" catalytic assay method for determination of koff values for kinase inhibitor drugs...
February 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28346105/high-throughput-clonogenic-analysis-of-3d-cultured-patient-derived-cells-with-a-micropillar-and-microwell-chip
#3
Dong Woo Lee, Sang-Yun Lee, Lily Park, Mi-Sun Kang, Myoung-Hee Kim, Il Doh, Gyu Ha Ryu, Do-Hyun Nam
A high-throughput clonogenic assay with a micropillar-microwell chip platform is proposed by using the colony area of glioblastoma multiforme (GBM) patient-derived cells (PDCs) from colony images. Unlike conventional cell lines, PDCs from the tumor are composed of heterogeneous cell populations, and some clonogenic populations form colonies during culture while the rest die off or remain unchanged, thus causing the diverse distribution of colony size. Therefore, area-based analysis of the total colonies is not sufficient to estimate total cell viability or toxicity responses...
February 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28346096/a-novel-multiparametric-drug-scoring-method-for-high-throughput-screening-of-3d-multicellular-tumor-spheroids-using-the-celigo-image-cytometer
#4
Scott Cribbes, Sarah Kessel, Scott McMenemy, Jean Qiu, Leo Li-Ying Chan
Three-dimensional (3D) tumor models have been increasingly used to investigate and characterize cancer drug compounds. The ability to perform high-throughput screening of 3D multicellular tumor spheroids (MCTS) can highly improve the efficiency and cost-effectiveness of discovering potential cancer drug candidates. Previously, the Celigo Image Cytometer has demonstrated a novel method for high-throughput screening of 3D multicellular tumor spheroids. In this work, we employed the Celigo Image Cytometer to examine the effects of 14 cancer drug compounds on 3D MCTS of the glioblastoma cell line U87MG in 384-well plates...
January 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28346093/a-label-free-continuous-fluorescence-based-assay-for-monitoring-ornithine-decarboxylase-activity-with-a-synthetic-putrescine-receptor
#5
Mohamed Nilam, Philip Gribbon, Jeanette Reinshagen, Kathrin Cordts, Edzard Schwedhelm, Werner M Nau, Andreas Hennig
Polyamines play an important role in cell growth, differentiation, and cancer development, and the biosynthetic pathway of polyamines is established as a drug target for the treatment of parasitic diseases, neoplasia, and cancer chemoprevention. The key enzyme in polyamine biosynthesis is ornithine decarboxylase (ODC). We report herein an analytical method for the continuous fluorescence monitoring of ODC activity based on the supramolecular receptor cucurbit[6]uril (CB6) and the fluorescent dye trans-4-[4-(dimethylamino)styryl]-1-methylpyridinium iodide (DSMI)...
January 1, 2017: SLAS Discov
https://www.readbyqxmd.com/read/28345797/metabolomics-strategy-for-the-mapping-of-volatile-exometabolome-from-saccharomyces-spp-widely-used-in-the-food-industry-based-on-comprehensive-two-dimensional-gas-chromatography
#6
Cátia Martins, Tiago Brandão, Adelaide Almeida, Sílvia M Rocha
Saccharomyces spp. are widely used in the food and beverages industries. Their cellular excreted metabolites are important for products' general quality and can contribute to product differentiation. This exploratory study presents a metabolomics strategy for the comprehensive mapping of cellular metabolites of two yeast species, Saccharomyces cerevisiae and S. pastorianus (both collected in an industrial context) through a multidimensional chromatography platform. Solid-phase microextraction was used as a sample preparation method...
March 27, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28345432/utero-placental-perfusion-doppler-indices-in-growth-restricted-fetuses-effect-of-sildenafil-citrate
#7
Mohamed Adel El-Sayed, Said Abdel-Aty Saleh, Mohammad Ahmed Maher, Asmaa Mohamed Khidre
OBJECTIVE: To assess efficacy and tolerability of sildenafil citrate on utero-placental blood flow and fetal growth in pregnancies complicated by fetal growth restriction (FGR). METHODS: From March 2015, a randomized controlled trial of 54 patients at 24 weeks or more complicated by FGR and abnormal Doppler indices were randomly allocated 1:1 into an intervention arm (receive sildenafil citrate, 50 mg) or a control arm (receive placebo). The primary outcomes were changes occurred in the Doppler parameters 2 h following drug administration...
March 27, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28344956/maternal-serum-placental-growth-factor-and-pregnancy-associated-plasma-protein-a-measured-in-the-first-trimester-as-parameters-of-subsequent-pre-eclampsia-and-small-for-gestational-age-infants-a-prospective-observational-study
#8
Kyung Uk Sung, Jeong A Roh, Kyung Jin Eoh, Eui Hyeok Kim
OBJECTIVE: To examine the first-trimester maternal serum placental growth factor (PlGF) and pregnancy-associated plasma protein A (PAPP-A) levels in pregnancies associated with pre-eclampsia (PE) or small-for-gestational-age (SGA) infants, and determine the predictive accuracy of PlGF and of PAPP-A for either PE or SGA infants. METHODS: This prospective, observational study included 175 pregnant women, and of these women, due to participant withdrawal or loss to follow-up, delivery data were collected from the medical records of 155 women, including 4 who had twin pregnancies...
March 2017: Obstetrics & Gynecology Science
https://www.readbyqxmd.com/read/28344584/lanthanum-affects-bell-pepper-seedling-quality-depending-on-the-genotype-and-time-of-exposure-by-differentially-modifying-plant-height-stem-diameter-and-concentrations-of-chlorophylls-sugars-amino-acids-and-proteins
#9
Atonaltzin García-Jiménez, Fernando C Gómez-Merino, Olga Tejeda-Sartorius, Libia I Trejo-Téllez
Lanthanum (La) is considered a beneficial element, capable of inducing hormesis. Hormesis is a dose-response relationship phenomenon characterized by low-dose stimulation and high-dose inhibition. Herein we tested the effect of 0 and 10 μM La on growth and biomolecule concentrations of seedlings of four sweet bell pepper (Capsicum annuum L.) varieties, namely Sven, Sympathy, Yolo Wonder, and Zidenka. Seedling evaluations were performed 15 and 30 days after treatment applications (dat) under hydroponic greenhouse conditions...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28344500/plant-growth-and-diosgenin-enhancement-effect-of-silver-nanoparticles-in-fenugreek-trigonella-foenum-graecum-l
#10
B Jasim, Roshmi Thomas, Jyothis Mathew, E K Radhakrishnan
Various methods have been used to enhance production of chemically diverse phytochemicals especially medicinal natural products. With the advancement in nanotechnology, nanoparticles have been reported to have varying impact in plant growth and inducibility of phytochemical composition. Major objective of the study was to study the secondary metabolite modulatory effect of silver nanoparticles. In the current study, treatment of fenugreek seedlings with biosynthesized silver nanoparticles (Ag-NPs) was found to have significant impact on its growth parameters such as leaf number, root length, shoot length and wet weight...
March 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28344396/effect-of-supplementation-of-lecithin-and-carnitine-on-growth-performance-and-nutrient-digestibility-in-pigs-fed-high-fat-diet
#11
Arathy Saseendran, K Ally, P Gangadevi, P S Banakar
AIM: To study the effect of dietary supplementation of lecithin and carnitine on growth performance and nutrient digestibility in pigs fed high-fat diet. MATERIALS AND METHODS: A total of 30 weaned female large white Yorkshire piglets of 2 months of age were selected and randomly divided into three groups allotted to three dietary treatments, T1 - Control ration as per the National Research Council nutrient requirement, T2 - Control ration plus 5% fat, and T3 - T2 plus 0...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28343353/development-reproduction-survival-and-demographic-patterns-of-tuta-absoluta-meyrick-lepidoptera-gelechiidae-on-different-commercial-tomato-cultivars
#12
F S Krechemer, L A Foerster
The increase in the production of tomato, Solanum lycopersicon Mill. (Solanaceae), has favored the proliferation of pests, especially Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). In this study, the development, reproduction, survival, and demographic parameters of T. absoluta reared on six commercial tomato cultivars (Cherry, Cordilheira, Giuliana, Nemoneta, Paron, and Santa Clara) were evaluated. Tuta absoluta completed its development in all tomato cultivars. Development from newly hatched caterpillar to newly emerged adult varied between 24...
March 25, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28343271/protein-degradation-systems-in-the-skeletal-muscles-of-parr-and-smolt-atlantic-salmon-salmo-salar-l-and-brown-trout-salmo-trutta-l
#13
Nadezda P Kantserova, Liudmila A Lysenko, Alexey E Veselov, Nina N Nemova
Although protein degradation limits the rate of muscle growth in fish, the role of proteolytic systems responsible for degrading myofibrillar proteins in skeletal muscle is not well defined. The study herein aims to evaluate the role of calpains (calcium-activated proteases) and proteasomes (ATP-dependent proteases) in mediating muscle protein turnover at different life stages in wild salmonids. Protease activities were estimated in Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.) parr and smolts from the Indera River (Kola Peninsula, Russia)...
March 26, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28343085/guava-fruit-extract-and-its-triterpene-constituents-have-osteoanabolic-effect-stimulation-of-osteoblast-differentiation-by-activation-of-mitochondrial-respiration-via-the-wnt-%C3%AE-catenin-signaling
#14
Konica Porwal, Subhashis Pal, Kapil Dev, Shyamsundar Pal China, Yogesh Kumar, Chandan Singh, Tarun Barbhuyan, Neeraj Sinha, Sabyasachi Sanyal, Arun Kumar Trivedi, Rakesh Maurya, Naibedya Chattopadhyay
The aim of this study was to evaluate the skeletal effect of guava triterpene-enriched extract (GE) in rats and identify osteogenic compounds thereof, and determine their modes of action. In growing female rats, GE at 250 mg/kg dose increased parameters of peak bone mass including femur length, bone mineral density (BMD) and biomechanical strength, suggesting that GE promoted modeling-directed bone growth. GE also stimulated bone regeneration at the site of bone injury. In adult osteopenic rats (osteopenia induced by ovariectomy, OVX) GE completely restored the lost bones at both axial and appendicular sites, suggesting a strong osteoanabolic effect...
March 8, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28343059/internal-cycling-not-external-loading-decides-the-nutrient-limitation-in-eutrophic-lake-a-dynamic-model-with-temporal-bayesian-hierarchical-inference
#15
Zhen Wu, Yong Liu, Zhongyao Liang, Sifeng Wu, Huaicheng Guo
Lake eutrophication is associated with excessive anthropogenic nutrients (mainly nitrogen (N) and phosphorus (P)) and unobserved internal nutrient cycling. Despite the advances in understanding the role of external loadings, the contribution of internal nutrient cycling is still an open question. A dynamic mass-balance model was developed to simulate and measure the contributions of internal cycling and external loading. It was based on the temporal Bayesian Hierarchical Framework (BHM), where we explored the seasonal patterns in the dynamics of nutrient cycling processes and the limitation of N and P on phytoplankton growth in hyper-eutrophic Lake Dianchi, China...
March 19, 2017: Water Research
https://www.readbyqxmd.com/read/28343049/stabilisation-of-spent-mushroom-substrate-for-application-as-a-plant-growth-promoting-organic-amendment
#16
Fabiana S Paula, Enrico Tatti, Florence Abram, Jude Wilson, Vincent O'Flaherty
Over three million tonnes of spent mushroom substrate (SMS) are produced in Europe every year as a by-product of the cultivation of Agaricus bisporus. The management of SMS has become an increasing challenge for the mushroom production industry, and finding environmentally and economically sustainable solutions for this organic residue is, therefore, highly desirable. Due to its physical properties and nutrient content, SMS has great potential to be employed in agricultural and horticultural sectors, and further contribute to reduce the use of non-renewable resources, such as peat...
March 23, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28342814/tear-cytokine-profile-of-glaucoma-patients-treated-with-preservative-free-or-preserved-latanoprost
#17
Jose M Martinez-de-la-Casa, Francisco Perez-Bartolome, Elena Urcelay, Jose L Santiago, Javier Moreno-Montañes, Pedro Arriola-Villalobos, Jose M Benitez-Del-Castillo, Julian Garcia-Feijoo
PURPOSE: To determine variations in cytokine levels of glaucoma patients treated either with preservative-free latanoprost or preserved latanoprost, relative to healthy individuals. METHODS: Tear samples were collected from 39 healthy subjects, 20 glaucoma patients treated with preserved latanoprost, and 20 patients treated with preservative-free latanoprost. A set of 27 inflammatory cytokines was analyzed in each group, including interleukin (IL)-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor (FGF) basic, granulocyte colony stimulating factor (G-CSF), granulocyte monocyte colony stimulating factor (GM-CSF), interferon (IFN)-γ, interferon gamma-induced protein (IP)-10, monocyte chemo attractant protein (MCP)-1MCAF, macrophage inflammatory protein (MIP)-1α, MIP-1β, platelet-derived growth factor (PDGF)-BB, regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF)...
March 22, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28342602/sex-effects-on-net-protein-and-energy-requirements-for-growth-of-saanen-goats
#18
A P Souza, N R St-Pierre, M H R M Fernandes, A K Almeida, J A C Vargas, K T Resende, I A M A Teixeira
Requirements for growth in the different sexes remain poorly quantified in goats. The objective of this study was to develop equations for estimating net protein (NPG) and net energy (NEG) for growth in Saanen goats of different sexes from 5 to 45 kg of body weight (BW). A data set from 7 comparative slaughter studies (238 individual records) of Saanen goats was used. Allometric equations were developed to determine body protein and energy contents in the empty BW (EBW) as dependent variables and EBW as the allometric predictor...
March 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28342418/dynamics-of-runoff-and-sediment-trapping-performance-of-vegetative-filter-strips-run-on-experiments-and-modeling
#19
Daili Pan, Xiaodong Gao, Miles Dyck, Yaqian Song, Pute Wu, Xining Zhao
Vegetative filter strips (VFSs) are a labor-saving and cost-effective agricultural best management practice to trap water runoff and sediment from the source areas. They also provide forage and/or fuel and are therefore potentially profitable for land owners. VFSs are however a dynamic system: the runoff delivery ratio (RDR) and sediment delivery ratio (SDR) vary with growth stage and vegetation types. The impacts of vegetation characteristics as well as soil physical properties modified by vegetation growth, on the RDR and SDR of VFS were evaluated by a flume experiment...
March 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28342374/accumulation-of-%C3%AE-hexabromocyclododecane-%C3%AE-hbcdd-in-tissues-of-fast-and-slow-growing-broilers-gallus-domesticus
#20
Catherine Jondreville, Ronan Cariou, Bertrand Méda, Elena Dominguez-Romero, Elsa Omer, Gaud Dervilly-Pinel, Bruno Le Bizec, Angélique Travel, Elisabeth Baéza
The aim of the current study was to describe the fate of ingested α-hexabromocyclododecane (α-HBCDD) in fast-growing (FG) and slow-growing (SG) broilers, through an exposure to a dietary concentration of 50 ng α-HBCDD g(-1) feed during 42 and 84 days, respectively. Depuration parameters were assessed in SG broilers successively exposed during 42 days and depurated during 42 days. At market age, SG broilers had ingested 42% more feed than FG broilers, while their body weight gain per g of feed ingested was 34% lower...
March 22, 2017: Chemosphere
keyword
keyword
56661
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"