keyword
MENU ▼
Read by QxMD icon Read
search

lipid modification and sequencing

keyword
https://www.readbyqxmd.com/read/28435686/apple-dwarfing-rootstocks-exhibit-an-imbalance-in-carbohydrate-allocation-and-reduced-cell-growth-and-metabolism
#1
Toshi M Foster, Peter A McAtee, Chethi N Waite, Helen L Boldingh, Tony K McGhie
Apple dwarfing rootstocks cause earlier shoot termination and reduced root and shoot mass. To identify physiological factors responsible for rootstock-induced growth restriction, we compared vascular-enriched gene expression between two dwarfing rootstocks ('M27' and 'M9') and the vigorous rootstock 'M793' using RNA sequencing and quantitative reverse transcriptase PCR. Differentially expressed genes common to both dwarfing rootstocks belonged to five main biological processes: (1) primary metabolism, (2) cell wall synthesis and modification, (3) secondary metabolism, (4) hormone signalling and response and (5) redox homeostasis...
2017: Horticulture Research
https://www.readbyqxmd.com/read/28433721/optimizing-the-restored-chemotactic-behavior-of-anticancer-agent-salmonella-enterica-serovar-typhimurium-vnp20009
#2
Katherine M Broadway, Seungbeum Suh, Bahareh Behkam, Birgit E Scharf
Bacteria, including strains of Salmonella, have been researched and applied as therapeutic cancer agents for centuries. Salmonella are particularly of interest due to their facultative anaerobic nature, facilitating colonization of differentially oxygenated tumor regions. Additionally, Salmonella can be manipulated with relative ease, resulting in the ability to attenuate the pathogen or engineer vectors for drug delivery. It was recently discovered that the anti-cancer Salmonella enterica serovar Typhimurium strain VNP20009 is lacking in chemotactic ability, due to a non-synonymous single nucleotide polymorphism in cheY...
April 19, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28395341/electrophilic-aldehyde-products-of-lipid-peroxidation-selectively-adduct-to-heat-shock-protein-90-and-arylsulfatase-a-in-stallion-spermatozoa%C3%A2
#3
Sally E Hall, R John Aitken, Brett Nixon, Nathan D Smith, Zamira Gibb
Oxidative stress is a major determinant of mammalian sperm function stimulating lipid peroxidation cascades that culminate in the generation of potentially cytotoxic aldehydes. The aim of this study was to assess the impact of such aldehydes on the functionality of stallion spermatozoa. The impact of exposure to exogenous acrolein (ACR) and 4-hydroxynonenal (4HNE) was manifested in a highly significant dose- and time-dependent increase in mitochondrial reactive oxygen species (ROS), total cellular ROS, a decrease in sperm motility, and a time-dependent increase in lipid peroxidation...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28377111/genome-wide-identification-of-micrornas-involved-in-fatty-acid-and-lipid-metabolism-of-brassica-napus-by-small-rna-and-degradome-sequencing
#4
Zhiwei Wang, Yan Qiao, Jingjing Zhang, Wenhui Shi, Jinwen Zhang
Rapeseed (Brassica napus) is an important cash crop considered as the third largest oil crop worldwide. Rapeseed oil contains various saturation or unsaturation fatty acids, these fatty acids, whose could incorporation with TAG form into lipids stored in seeds play various roles in the metabolic activity. The different fatty acids in B. napus seeds determine oil quality, define if the oil is edible or must be used as industrial material. miRNAs are kind of non-coding sRNAs that could regulate gene expressions through post-transcriptional modification to their target transcripts playing important roles in plant metabolic activities...
April 1, 2017: Gene
https://www.readbyqxmd.com/read/28373551/identification-of-specific-posttranslational-o-mycoloylations-mediating-protein-targeting-to-the-mycomembrane
#5
Clément Carel, Julien Marcoux, Valérie Réat, Julien Parra, Guillaume Latgé, Françoise Laval, Pascal Demange, Odile Burlet-Schiltz, Alain Milon, Mamadou Daffé, Maryelle G Tropis, Marie A M Renault
The outer membranes (OMs) of members of the Corynebacteriales bacterial order, also called mycomembranes, harbor mycolic acids and unusual outer membrane proteins (OMPs), including those with α-helical structure. The signals that allow precursors of such proteins to be targeted to the mycomembrane remain uncharacterized. We report here the molecular features responsible for OMP targeting to the mycomembrane of Corynebacterium glutamicum, a nonpathogenic member of the Corynebacteriales order. To better understand the mechanisms by which OMP precursors were sorted in C...
April 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28344321/broad-spectrum-antiviral-activity-for-paramyxoviruses-is-modulated-by-biophysical-properties-of-fusion-inhibitory-peptides
#6
Cyrille Mathieu, Marcelo T Augusto, Stefan Niewiesk, Branka Horvat, Laura M Palermo, Giuseppina Sanna, Silvia Madeddu, Devra Huey, Miguel A R B Castanho, Matteo Porotto, Nuno C Santos, Anne Moscona
Human paramyxoviruses include global causes of lower respiratory disease like the parainfluenza viruses, as well as agents of lethal encephalitis like Nipah virus. Infection is initiated by viral glycoprotein-mediated fusion between viral and host cell membranes. Paramyxovirus viral fusion proteins (F) insert into the target cell membrane, and form a transient intermediate that pulls the viral and cell membranes together as two heptad-repeat regions refold to form a six-helix bundle structure that can be specifically targeted by fusion-inhibitory peptides...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28323072/impairment-of-the-class-iia-bacteriocin-receptor-function-and-membrane-structural-changes-are-associated-to-enterocin-crl35-high-resistance-in-listeria-monocytogenes
#7
Emilse Masias, Fernando G Dupuy, Paulo Ricardo da Silva Sanches, Juan Vicente Farizano, Eduardo Cilli, Augusto Bellomio, Lucila Saavedra, Carlos Minahk
BACKGROUND: Enterocin CRL35 is a class IIa bacteriocin with anti-Listeria activity. Resistance to these peptides has been associated with either the downregulation of the receptor expression or changes in the membrane and cell walls. The scope of the present work was to characterize enterocin CRL35 resistant Listeria strains with MICs more than 10,000 times higher than the MIC of the WT sensitive strain. METHODS: Listeria monocytogenes INS7 resistant isolates R2 and R3 were characterized by 16S RNA gene sequencing and rep-PCR...
March 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28301719/a-reconfigured-kennedy-pathway-which-promotes-efficient-accumulation-of-medium-chain-fatty-acids-in-leaf-oils
#8
Kyle B Reynolds, Matthew C Taylor, Darren P Cullerne, Christopher L Blanchard, Craig C Wood, Surinder P Singh, James R Petrie
Medium chain fatty acids (MCFA, C6-14 fatty acids) are an ideal feedstock for biodiesel and broader oleochemicals. In recent decades, several studies have used transgenic engineering to produce MCFA in seeds oils, though these modifications result in unbalance membrane lipid profiles that impair oil yields and agronomic performance. Given the ability to engineer non-seed organs to produce oils, we have previously demonstrated that MCFA profiles can be produced in leaves, but this also results in unbalanced membrane lipid profiles and undesirable chlorosis and cell death...
March 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28286565/everolimus-in-the-management-of-metastatic-neuroendocrine-tumours
#9
REVIEW
David L Chan, Eva Segelov, Simron Singh
Neuroendocrine tumours are increasing in incidence and cause a variety of symptoms. The mammalian target of rapamycin (mTOR) pathway plays a key role in neuroendocrine tumour (NET) pathogenesis, leading to increased lipid synthesis, protein synthesis and cellular growth. Upregulation of this pathway is noted in both hereditary and sporadic NETs. This understanding has led to investigations of mTOR inhibitors as therapy for metastatic NETs. After promising preclinical findings, everolimus, an mTOR inhibitor, was trialled in the RADIANT-1-4 studies on patients with advanced, well differentiated NETs...
January 2017: Therapeutic Advances in Gastroenterology
https://www.readbyqxmd.com/read/28259411/genetic-and-functional-analysis-of-the-bovine-uterine-microbiota-part-ii-purulent-vaginal-discharge-versus-healthy-cows
#10
M L S Bicalho, S Lima, C H Higgins, V S Machado, F S Lima, R C Bicalho
The aim of this study was to characterize, using metagenomic shotgun DNA sequencing, the intrauterine microbial population and its predicted functional diversity within healthy cows and cows presenting purulent vaginal discharge (PVD). Twenty Holstein dairy cows from a single farm were enrolled in the study at 25 to 35 d postpartum. Purulent vaginal discharge was diagnosed by retrieving and scoring vaginal discharge using the Metricheck device (Simcro, Hamilton, New Zealand). Intrauterine samples for metagenomic analysis were collected by the cytobrush technique from 8 cows diagnosed with PVD and 12 healthy cows...
May 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28209172/evaluation-of-the-contribution-of-the-transmembrane-region-to-the-ectodomain-conformation-of-the-human-immunodeficiency-virus-hiv-1-envelope-glycoprotein
#11
Hanh T Nguyen, Navid Madani, Haitao Ding, Emerald Elder, Amy Princiotto, Christopher Gu, Patrice Darby, James Alin, Alon Herschhorn, John C Kappes, Youdong Mao, Joseph G Sodroski
BACKGROUND: The human immunodeficiency virus (HIV-1) envelope glycoprotein (Env), a Type 1 transmembrane protein, assembles into a trimeric spike complex that mediates virus entry into host cells. The high potential energy of the metastable, unliganded Env trimer is maintained by multiple non-covalent contacts among the gp120 exterior and gp41 transmembrane Env subunits. Structural studies suggest that the gp41 transmembrane region forms a left-handed coiled coil that contributes to the Env trimer interprotomer contacts...
February 16, 2017: Virology Journal
https://www.readbyqxmd.com/read/28207798/altered-hepatic-lipid-metabolism-in-mice-lacking-both-the-melanocortin-type-4-receptor-and-low-density-lipoprotein-receptor
#12
Vera Lede, Andrej Meusel, Antje Garten, Yulia Popkova, Melanie Penke, Christin Franke, Albert Ricken, Angela Schulz, Wieland Kiess, Daniel Huster, Torsten Schöneberg, Jürgen Schiller
Obesity is often associated with dyslipidemia and hepatosteatosis. A number of animal models of non-alcoholic fatty liver disease (NAFLD) are established but they significantly differ in the molecular and biochemical changes depending on the genetic modification and diet used. Mice deficient for melanocortin type 4 receptor (Mc4rmut) develop hyperphagia, obesity, and subsequently NAFLD already under regular chow and resemble more closely the energy supply-driven obesity found in humans. This animal model was used to assess the molecular and biochemical consequences of hyperphagia-induced obesity on hepatic lipid metabolism...
2017: PloS One
https://www.readbyqxmd.com/read/28130120/trifluoroacetylated-tyrosine-rich-d-tetrapeptides-have-potent-antioxidant-activity
#13
Annamaria Sandomenico, Valeria Severino, Fabio Apone, Adriana De Lucia, Andrea Caporale, Nunzianna Doti, Anna Russo, Rosita Russo, Camilla Rega, Tiziana Del Giacco, Lucia Falcigno, Menotti Ruvo, Angela Chambery
The term "oxidative stress" indicates a set of chemical reactions unleashed by a disparate number of events inducing DNA damage, lipid peroxidation, protein modification and other effects, which are responsible of altering the physiological status of cells or tissues. Excessive Reactive Oxygen Species (ROS) levels may accelerate ageing of tissues or induce damage of biomolecules thus promoting cell death or proliferation in dependence of cell status and of targeted molecules. In this context, new antioxidants preventing such effects may have a relevant role as modulators of cell homeostasis and as therapeutic agents...
March 2017: Peptides
https://www.readbyqxmd.com/read/28122677/colistin-and-carbapenem-resistant-klebsiella-oxytoca-harboring-blavim-2-and-an-insertion-in-the-mgrb-gene-isolated-from-blood-culture
#14
Michaela Simon, Holger Melzl, Andreas Hiergeist, Katharina Richert, Linda Falgenhauer, Yvonne Pfeifer, Roman G Gerlach, Kornelius Fuchs, Udo Reischl, André Gessner, Jonathan Jantsch
A carbapenemase-producing colistin-resistant Klebsiella oxytoca isolate was recovered from a blood culture of a female patient without previous report of risk factors to obtain multidrug-resistant Gram-negative bacilli. A combination of biochemical and molecular methods was used to identify the resistance mechanism of this isolate. Carbapenemase production was mediated by Verona integron-encoded metallo-β-lactamase (VIM)-2. Colistin resistance was not due to plasmid- borne mcr-1 gene, but we found an integration of IS5-like sequence in the mgrB gene of K...
February 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28114274/akt3-is-a-privileged-first-responder-in-isozyme-specific-electrophile-response
#15
Marcus J C Long, Saba Parvez, Yi Zhao, Sanjna L Surya, Yiran Wang, Sheng Zhang, Yimon Aye
Isozyme-specific post-translational regulation fine tunes signaling events. However, redundancy in sequence or activity renders links between isozyme-specific modifications and downstream functions uncertain. Methods to study this phenomenon are underdeveloped. Here we use a redox-targeting screen to reveal that Akt3 is a first-responding isozyme sensing native electrophilic lipids. Electrophile modification of Akt3 modulated downstream pathway responses in cells and Danio rerio (zebrafish) and markedly differed from Akt2-specific oxidative regulation...
March 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28000567/analysis-and-prediction-of-myristoylation-sites-using-the-mrmr-method-the-ifs-method-and-an-extreme-learning-machine-algorithm
#16
ShaoPeng Wang, Yu-Hang Zhang, Guohua Huang, Lei Chen, Yu-Dong Cai
Myristoylation is an important hydrophobic post-translational modificationthat is covalently bound tothe amino group of Gly residueson the N-terminus of proteins. The many diversefunctions of myristoylation on proteins,such as membrane targeting, signal pathway regulation and apoptosis,are largely due to the lipid modification,whereasabnormal or irregular myristoylation on proteins can lead to several pathological changes in the cell. To better understand the function o fmyristoylated sites and to correctly identify them in protein sequences, this study conducted a novel investigation of myristoylation sites...
December 20, 2016: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/27988885/lipid-metabolites-in-seeds-of-diverse-gossypium-accessions-molecular-identification-of-a-high-oleic-mutant-allele
#17
Drew Sturtevant, Patrick Horn, Christopher Kennedy, Lori Hinze, Richard Percy, Kent Chapman
Genetically diverse cottonseeds show altered compositions and spatial distributions of phosphatidylcholines and triacylglycerols. Lipidomics profiling led to the discovery of a novel FAD2 - 1 allele, fad2 - 1D - 1 , resulting in a high oleic phenotype. The domestication and breeding of cotton for elite, high-fiber cultivars have led to reduced variation of seed constituents within currently cultivated upland cotton genotypes. However, a recent screen of the genetically diverse U.S. National Cotton Germplasm Collection identified Gossypium accessions with marked differences in seed oil and protein content...
December 17, 2016: Planta
https://www.readbyqxmd.com/read/27930681/transcriptomic-changes-in-liver-of-young-bulls-caused-by-diets-low-in-mineral-and-protein-contents-and-supplemented-with-n-3-fatty-acids-and-conjugated-linoleic-acid
#18
Sara Pegolo, Alessio Cecchinato, Núria Mach, Massimiliano Babbucci, Marianna Pauletto, Luca Bargelloni, Stefano Schiavon, Giovanni Bittante
The aim of the present study was to identify transcriptional modifications and regulatory networks accounting for physiological and metabolic responses to specific nutrients in the liver of young Belgian Blue × Holstein bulls using RNA-sequencing. A larger trial has been carried out in which animals were fed with different diets: 1] a conventional diet; 2] a low-protein/low-mineral diet (low-impact diet) and 3] a diet enriched in n-3 fatty acids (FAs), conjugated linoleic acid (CLA) and vitamin E (nutraceutical diet)...
2016: PloS One
https://www.readbyqxmd.com/read/27899336/complete-genome-sequence-of-stenotrophomonas-sp-kacc-91585-an-efficient-bacterium-for-unsaturated-fatty-acid-hydration
#19
Kyoung-Rok Kim, Woo-Ri Kang, Deok-Kun Oh
Hydroxy fatty acids (HFAs) such as 10-hydroxystearic acid (10-HSA) and 10-hydroxy-12(Z)-octadecenoic acid (10-HOD), which are similar to ricinoleic acid, are important starting materials and intermediates for the industrial manufacture of many commodities. Stenotrophomonas sp. KACC 91585, which was isolated from lake sediment, is an efficient bacterium for unsaturated fatty acid hydration that produces 10-HSA and 10-HOD from oleic acid and linoleic acid, respectively, with high conversion rates. The complete genome of this strain is 4,541,729bp with 63...
January 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/27870114/the-molecular-genetics-of-autosomal-recessive-nonsyndromic-intellectual-disability-a-mutational-continuum-and-future-recommendations
#20
REVIEW
Muzammil Ahmad Khan, Saadullah Khan, Christian Windpassinger, Muhammad Badar, Zafar Nawaz, Ramzi M Mohammad
Intellectual disability (ID) is a clinical manifestation of the central nervous system without any major dysmorphologies of the brain. Biologically it affects learning capabilities, memory, and cognitive functioning. The basic defining features of ID are characterized by IQ<70, age of onset before 18 years, and impairment of at least two of the adaptive skills. Clinically it is classified in a syndromic (with additional abnormalities) and a nonsyndromic form (with only cognitive impairment). The study of nonsyndromic intellectual disability (NSID) can best explain the pathophysiology of cognition, intelligence and memory...
November 2016: Annals of Human Genetics
keyword
keyword
56642
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"