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https://www.readbyqxmd.com/read/28078781/a-novel-conversion-method-for-radiographic-guide-into-surgical-guide
#1
Yao-Te Peng, Chung-Chih Tseng, Yi-Chun Du, Yen-Nien Chen, Chih-Han Chang
BACKGROUND: The study proposed a novel method for converting a radiographic guide into a surgical guide and evaluated its accuracy. MATERIALS AND METHODS: Radiographic guide was reformed with the addition of index rods for geometric conversion method (GCM). Planning implants were projected on geometric projection planes, and the implant positions were measured. The radiographic guide was converted into surgical guide using a generic bench drill machine with GCM data...
January 11, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/28077588/first-in-human-evaluation-of-the-safety-and-immunogenicity-of-an-intranasally-administered-replication-competent-sendai-virus-vectored-hiv-type-1-gag-vaccine-induction-of-potent-t-cell-or-antibody-responses-in-prime-boost-regimens
#2
Julien Nyombayire, Omu Anzala, Brian Gazzard, Etienne Karita, Philip Bergin, Peter Hayes, Jakub Kopycinski, Gloria Omosa-Manyonyi, Akil Jackson, Jean Bizimana, Bashir Farah, Eddy Sayeed, Christopher L Parks, Makoto Inoue, Takashi Hironaka, Hiroto Hara, Tsugumine Shu, Tetsuro Matano, Len Dally, Burc Barin, Harriet Park, Jill Gilmour, Angela Lombardo, Jean-Louis Excler, Patricia Fast, Dagna S Laufer, Josephine H Cox
BACKGROUND:  We report the first-in-human safety and immunogenicity assessment of a prototype intranasally administered, replication-competent Sendai virus (SeV)-vectored, human immunodeficiency virus type 1 (HIV-1) vaccine. METHODS:  Sixty-five HIV-1-uninfected adults in Kenya, Rwanda, and the United Kingdom were assigned to receive 1 of 4 prime-boost regimens (administered at 0 and 4 months, respectively; ratio of vaccine to placebo recipients, 12:4): priming with a lower-dose SeV-Gag given intranasally, followed by boosting with an adenovirus 35-vectored vaccine encoding HIV-1 Gag, reverse transcriptase, integrase, and Nef (Ad35-GRIN) given intramuscularly (SLA); priming with a higher-dose SeV-Gag given intranasally, followed by boosting with Ad35-GRIN given intramuscularly (SHA); priming with Ad35-GRIN given intramuscularly, followed by boosting with a higher-dose SeV-Gag given intranasally (ASH); and priming and boosting with a higher-dose SeV-Gag given intranasally (SHSH)...
January 1, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28075047/growing-larger-with-domestication-a-matter-of-physiology-morphology-or-allocation
#3
R Milla, S Matesanz
• Domestication might affect plant size. We investigated whether herbaceous crops are larger than their wild progenitors, and the traits that influence size variation. • We grew six crop plants and their wild progenitors under common garden conditions. We measured the aboveground biomass gain by individual plants during the vegetative stage. We then tested whether the photosynthesis rate, biomass allocation to leaves, leaf size, and specific leaf area (SLA) accounted for variations in whole-plant photosynthesis, and ultimately in aboveground biomass...
January 11, 2017: Plant Biology
https://www.readbyqxmd.com/read/28061998/effects-of-mycoplasma-hyopneumoniae-on-porcine-nasal-cavity-dendritic-cells
#4
Yumeng Shen, Weiwei Hu, Yanna Wei, Zhixin Feng, Qian Yang
Mycoplasma hyopneumoniae (Mhp) is the primary etiological agent responsible for swine enzootic pneumonia (EP), a disease that cause tremendous economic losses all over the swine industry. Dendritic cells (DCs), the most effective antigen-presenting cells, are widely distributed beneath respiratory epithelium. DCs uptake and present antigens to T cells, to initiate protective immune responses or generate immune-mediated pathology in different infections. In this study, we investigated the changes in the different DCs subpopulations, T cells and SIgA positive cells counts in porcine nasal cavity after long time Mhp infection...
January 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28054449/drought-responses-phenotypic-plasticity-and-survival-of-mediterranean-species-in-two-different-microclimatic-sites
#5
Franca J Bongers, Manuel Olmo, Barbara Lopez-Iglesias, Niels P R Anten, Rafael Villar
Climate models predict a further drying of the Mediterranean summer. One way for plant species to persist during such climatic changes is through acclimation. Here, we determine the extent to which trait plasticity in response to drought differs between species and between sites, and address the question whether there is a trade-off between drought survival and phenotypic plasticity. Through the summer we measured physiological traits (Photosynthesis - Amax , stomatal conductance - gs , transpiration - E, leaf water potential - ψl) and structural traits (specific leaf area - SLA, leaf density - LD, leaf dry matter content - LDMC, leaf relative water content - LRWC) of leaves of eight woody species in two sites with slightly different microclimate (north versus south-facing slopes) in southern Spain...
January 5, 2017: Plant Biology
https://www.readbyqxmd.com/read/28053904/comparative-study-on-the-osseointegration-of-implants-in-dog-mandibles-according-to-the-implant-surface-treatment
#6
Wook-Jae Yoon, Su-Gwan Kim, Ji-Su Oh, Jae-Seek You, Kyung-In Jeong, Sung-Chul Lim, Mi-Ae Jeong
OBJECTIVES: This study compared the impact of implant surface treatment on the stability and osseointegration of implants in dog mandibles. MATERIALS AND METHODS: Six adult dogs received a total of 48 implants that were prepared using four different surface treatments; resorbable blast media (RBM), hydroxyapatite (HA), hydrothermal-treated HA, and sand blasting and acid etching (SLA). Implants were installed, and dogs were separated into 2- and 4-week groups. Implant stability was evaluated via Periotest M, Osstell Mentor, and removal torque analyzers...
December 2016: Journal of the Korean Association of Oral and Maxillofacial Surgeons
https://www.readbyqxmd.com/read/28053571/comparative-study-of-oxidative-stress-parameters-and-acetylcholinesterase-activity-in-the-liver-of-pelophylax-esculentus-complex-frogs
#7
Marko Prokić, Slavica Borković-Mitić, Imre Krizmanić, Jelena Gavrić, Svetlana Despotović, Branka Gavrilović, Tijana Radovanović, Slađan Pavlović, Zorica Saičić
Comparative activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), the phase II biotransformation enzyme glutathione-S-transferase (GST), the concentrations of total glutathione (GSH), sulfhydryl groups (-SH) and the activity of the neurotoxicity biomarker acetylcholinesterase (AChE) were investigated in the livers of species belonging to the Pelophylax esculentus "complex" (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax kl...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28052395/herbivores-sculpt-leaf-traits-differently-in-grasslands-depending-on-life-form-and-land-use-histories
#8
Jennifer Firn, Martin Schütz, Huong Nguyen, Anita C Risch
Vertebrate and invertebrate herbivores alter plant communities directly by selectively consuming plant species; and indirectly by inducing morphological and physiological changes to plant traits that provide competitive or survivorship advantages to some life forms over others. Progressively excluding aboveground herbivore communities (ungulates, medium and small sized mammals, invertebrates) over five growing seasons, we explored how leaf morphology (specific leaf area or SLA) and nutrition (nitrogen, carbon, phosphorous, potassium, sodium, and calcium) of different plant life forms (forbs, legumes, grasses, sedges) correlated with their dominance...
January 2017: Ecology
https://www.readbyqxmd.com/read/28043034/oxidative-stress-biomarkers-cholinesterase-activity-and-biotransformation-enzymes-in-the-liver-of-dice-snake-natrix-tessellata-laurenti-during-pre-hibernation-and-post-hibernation-a-possible-correlation-with-heavy-metals-in-the-environment
#9
Jelena Gavrić, Marko Anđelković, Ljiljana Tomović, Marko Prokić, Svetlana Despotović, Branka Gavrilović, Tijana Radovanović, Slavica Borković-Mitić, Slađan Pavlović, Zorica Saičić
We investigated in the liver of dice snakes during pre- and post-hibernation changes in the following antioxidant parameters: total, manganese and copper zinc containing superoxide dismutases (Tot SOD, MnSOD, CuZn SOD, respectively), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the concentrations of total glutathione (GSH) and sulfhydryl groups (-SH). In addition, we examined the expression of phase I biotransformation enzyme cytochrome P4501A (CYP1A) and the activity of phase II biotransformation enzyme glutathioneS-transferase (GST), the level of lipid peroxidation (by measuring the thiobarbituric acid-reactive substances (TBARS)), cholinesterase activity (ChE) and metallothionein expression (MT)...
December 30, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28042263/efficacy-of-la-axxess-burs-gates-glidden-drills-and-protaper-sx-in-obtaining-straight-line-access-in-mesiobuccal-roots-of-mandibular-first-molars-a-cone-beam-computed-tomography-assessment
#10
Promila Verma, Rhythm Bains, A P Tikku, Anil Chandra, Shibha Mehta
OBJECTIVE: This study aims at cone-beam computed tomography (CBCT) evaluation of the ability of Gates Glidden (GG) drills, Protaper Sx, and LA Axxess burs to produce a straight line access (SLA) in mesiobuccal canals of mandibular first molars. METHODOLOGY: Forty-five freshly extracted mandibular teeth with a canal curvature of 10-20° were taken for the study and divided into three groups according to the instruments used for cervical preflaring: Group I (LA Axxess burs), Group II (GG drills), and Group III (Protaper Sx)...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28035276/nitrogen-phosphorous-supply-ratio-and-allometry-in-five-alpine-plant-species
#11
Xi Luo, Susan J Mazer, Hui Guo, Nan Zhang, Jacob Weiner, Shuijin Hu
: In terrestrial ecosystems, atmospheric nitrogen (N) deposition has greatly increased N availability relative to other elements, particularly phosphorus (P). Alterations in the availability of N relative to P can affect plant growth rate and functional traits, as well as resource allocation to above- versus belowground biomass (MA and MB). Biomass allocation among individual plants is broadly size-dependent, and this can often be described as an allometric relationship between MA and MB, as represented by the equation MA=αMBβ, or log MA = logα + βlog MB...
December 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/28024815/fit-of-pressed-crowns-fabricated-from-two-cad-cam-wax-pattern-process-plans-a-comparative-in%C3%A2-vitro-study
#12
Loubna Shamseddine, Rola Mortada, Khaldoun Rifai, Jose Johann Chidiac
STATEMENT OF PROBLEM: Subtractive and additive computer-aided design and computer-aided manufacturing (CAD-CAM) wax pattern processing are 2 methods of fabricating a pressed ceramic crown. Whether a subtractive milled wax pattern or a pattern from the micro-stereolithography additive process produces lithium disilicate crowns with better marginal and internal fit is unclear. MATERIALS AND METHODS: Ten silicone impressions were made for a prepared canine tooth. Each die received 2 lithium disilicate (IPS e...
December 23, 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28012063/the-influence-of-implantoplasty-on-the-diameter-chemical-surface-composition-and-biocompatibility-of-titanium-implants
#13
Frank Schwarz, Gordon John, Jürgen Becker
OBJECTIVES: The objective of the study was to assess the influence of implantoplasty (IP) on the diameter, chemical surface composition, and biocompatibility of titanium implants in vitro. MATERIAL AND METHODS: Twenty soft tissue-level (TL; machined transmucosal-M and rough endosseous part-SLA) and 20 bone-level (BL; SLA) implants were allocated to IP covering 3 or 6 mm of the structured surface (SLA) area. The samples were subjected to diameter, energy-dispersive X-ray spectroscopy (EDX), and cell viability (ginigval fibroblasts, 6 days) assessments...
December 24, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28003480/non-structural-protein-4-of-porcine-reproductive-and-respiratory-syndrome-virus-modulates-cell-surface-swine-leukocyte-antigen-class-i-expression-by-down-regulating-%C3%AE-2-microglobulin-transcription
#14
Pengfei Qi, Ke Liu, Jianchao Wei, Yuming Li, Beibei Li, Donghua Shao, Zhuanchang Wu, Yuanyuan Shi, Guangzhi Tong, Yafeng Qiu, Zhiyong Ma
: Porcine reproductive and respiratory syndrome (PPRS) virus (PRRSV) is the causative agent of PRRS, which has important impacts on the pig industry. PRRSV infection results in disruption of the swine leukocyte antigen class I (SLA-I) antigen presentation pathway. In this study, highly pathogenic PRRSV (HP-PRRSV) infection inhibited transcription of the β2-microglobulin (β2M) gene (B2M), and reduced cellular levels of β2M, which forms a heterotrimeric complex with SLA-I heavy chain and a variable peptide, and plays a critical role in SLA-I antigen presentation...
December 21, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27995703/shade-tolerance-and-herbivory-are-associated-with-rgr-of-tree-species-via-different-functional-traits
#15
Cristian Salgado-Luarte, Ernesto Gianoli
Relative growth rate (RGR) plays an important role in plant adaptation to the light environment through the growth potential/survival trade-off. RGR is a complex trait with physiological and biomass allocation components. It has been argued that herbivory may influence the evolution of plant strategies to cope with the light environment, but little is known about the relation between susceptibility to herbivores and growth-related functional traits. Here, we examined in 11 evergreen tree species from a temperate rainforest the association between growth-related functional traits and (i) species' shade-tolerance, and (ii) herbivory rate in the field...
December 20, 2016: Plant Biology
https://www.readbyqxmd.com/read/27994142/regulatory-module-involving-fgf13-mir-504-and-p53-regulates-ribosomal-biogenesis-and-supports-cancer-cell-survival
#16
Débora R Bublik, Slađana Bursać, Michal Sheffer, Ines Oršolić, Tali Shalit, Ohad Tarcic, Eran Kotler, Odelia Mouhadeb, Yonit Hoffman, Gilad Fuchs, Yishai Levin, Siniša Volarević, Moshe Oren
The microRNA miR-504 targets TP53 mRNA encoding the p53 tumor suppressor. miR-504 resides within the fibroblast growth factor 13 (FGF13) gene, which is overexpressed in various cancers. We report that the FGF13 locus, comprising FGF13 and miR-504, is transcriptionally repressed by p53, defining an additional negative feedback loop in the p53 network. Furthermore, we show that FGF13 1A is a nucleolar protein that represses ribosomal RNA transcription and attenuates protein synthesis. Importantly, in cancer cells expressing high levels of FGF13, the depletion of FGF13 elicits increased proteostasis stress, associated with the accumulation of reactive oxygen species and apoptosis...
December 19, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27990284/a-structure-function-approach-to-optimizing-tlr4-ligands-for-human-vaccines
#17
Darrick Carter, Christopher B Fox, Tracey A Day, Jeffrey A Guderian, Hong Liang, Tom Rolf, Julie Vergara, Zachary K Sagawa, Greg Ireton, Mark T Orr, Anthony Desbien, Malcolm S Duthie, Rhea N Coler, Steven G Reed
Adjuvants are combined with vaccine antigens to enhance and modify immune responses, and have historically been primarily crude, undefined entities. Introducing toll-like receptor (TLR) ligands has led to a new generation of adjuvants, with TLR4 ligands being the most extensively used in human vaccines. The TLR4 crystal structures demonstrate extensive contact with their ligands and provide clues as to how they discriminate a broad array of molecules and activate or attenuate innate, as well as adaptive, responses resulting from these interactions...
November 2016: Clinical & Translational Immunology
https://www.readbyqxmd.com/read/27987735/combined-effect-of-grain-refinement-and-surface-modification-of-pure-titanium-on-the-attachment-of-mesenchymal-stem-cells-and-osteoblast-like-saos-2-cells
#18
A E Medvedev, A Neumann, H P Ng, R Lapovok, C Kasper, T C Lowe, V N Anumalasetty, Y Estrin
Surface modification is an important step in production of medical implants. Surface roughening creates additional surface area to enhance the bonding between the implant and the bone. Recent research provided a means to alter the microstructure of titanium by severe plastic deformation (SPD) in order to increase its strength, and thereby reduce the size of the implants (specifically, their diameter). The purpose of the present study was to examine the effect of bulk microstructure of commercially pure titanium with coarse-grained (CG) and ultrafine-grained (UFG) bulk structure on the surface state of these materials after surface modification by sand blasting and acid etching (SLA)...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27981788/plasticity-in-seedling-morphology-biomass-allocation-and-physiology-among-ten-temperate-tree-species-in-response-to-shade-is-related-to-shade-tolerance-and-not-leaf-habit
#19
Daniel J Chmura, Jerzy Modrzyński, Paweł Chmielarz, Mark G Tjoelker
Mechanisms of shade tolerance in tree seedlings, and thus growth in shade, may differ by leaf habit and vary with ontogeny following seed germination. To examine early responses of seedlings to shade in relation to morphological, physiological and biomass allocation traits, we compared seedlings of ten temperate species, varying in their leaf habit (broadleaved vs. needleleaved) and observed tolerance to shade, growing in two contrasting light treatments - open (about 20% of full sunlight), and shade (about 5% of full sunlight)...
December 16, 2016: Plant Biology
https://www.readbyqxmd.com/read/27974250/the-influence-of-genetic-predisposition-and-autoimmune-hepatitis-inducing-antigens-in-disease-development
#20
Matthias Hardtke-Wolenski, Janine Dywicki, Katja Fischer, Martin Hapke, Maren Sievers, Jerome Schlue, Mark S Anderson, Richard Taubert, Fatih Noyan, Michael P Manns, Elmar Jäckel
: Autoimmune hepatitis (AIH) is defined as a chronic liver inflammation with loss of tolerance against hepatocytes. The etiology and pathophysiology of AIH are still poorly understood because reliable animal models are limited. Therefore, we recently introduced a model of experimental murine AIH by a self-limited adenoviral infection with the AIH type 2 antigen formiminotransferase cyclodeaminase (FTCD). We could demonstrate that break of humoral tolerance towards liver specific autoantigens like FTCD and cytochrome P450 2D6 (CYP2D6) is not dependent on the genetic background...
December 11, 2016: Journal of Autoimmunity
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