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Elise A Lamont, Erin Poulin, Srinand Sreevatsan, Maxim C-J Cheeran
Influenza A virus in swine (IAV-S) is a prevalent respiratory pathogen in pigs that has deleterious consequences to animal and human health. Pigs represent an important reservoir for influenza and potential mixing vessel for novel gene reassortments. Despite the central role of pigs in recent influenza outbreaks, much remains unknown about the impact of swine immunity on IAV-S transmission, pathogenesis, and evolution. An incomplete understanding of interactions between the porcine immune system and IAV-S has hindered development of new diagnostic tools and vaccines...
January 12, 2018: Journal of General Virology
M D Jiménez, R de Torre, I Mola, M A Casado, L Balaguer
The growing number of road vehicles is a major source of regional and global atmospheric pollution increasing concentrations of CO2 in the air, and levels of metals in air and soil. Nevertheless, the effects of these pollutants on plants growing at roadsides are poorly documented. We carried out an observational study of unmanipulated plants growing by the road, to identify the morpho-physiological responses in a perennial grass Dactylis glomerata. Firstly, we wanted to know the general effect of traffic intensity and ambient CO2 and its interactions on different plant traits...
February 15, 2018: Journal of Environmental Management
Božidar Rašković, Vesna Poleksić, Stefan Skorić, Katarina Jovičić, Slađana Spasić, Aleksandar Hegediš, Nebojša Vasić, Mirjana Lenhardt
Chub (Squalius cephalus) specimens were collected in Korenita River seven months after spillover from the waste water of antimony mine tailing pond and compared with chub living in Kruščica reservoir (intended for water supply) and Međuvršje reservoir (influenced by intense emission of industrial, urban and rural wastewater). Concentrations of 15 elements (Al, As, B, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Si, Sr, Zn) were determined in muscle, liver and gills of chub by inductively coupled plasma optical spectrometry (ICP-OES) and histopathological alterations in liver and gills were assessed...
February 14, 2018: Ecotoxicology and Environmental Safety
Martí Cortey, Gaston Arocena, Tahar Ait-Ali, Anna Vidal, Yanli Li, Gerard Martín-Valls, Alison D Wilson, Allan L Archibald, Enric Mateu, Laila Darwich
Porcine reproductive and respiratory syndrome virus (PRRSv) infection alters the host's cellular and humoral immune response. Immunity against PRRSv is multigenic and vary between individuals. The aim of the present study was to compare several genes that encode for molecules involved in the immune response between two groups of vaccinated pigs that experienced short or long viremic periods after PRRSv challenge. These analyses include the sequencing of four SLA Class I, two Class II allele groups, and CD163, plus the analysis by quantitative realtime qRT-PCR of the constitutive expression of TLR2, TLR3, TLR4, TLR7, TLR8 and TLR9 mRNA and other molecules in peripheral blood mononuclear cells...
February 15, 2018: Veterinary Research
Myron Nevins, Stefano Parma-Benfenati, Cosmin Sava, Catalin Sava, Franco Quinti, Primo Galletti, Gerardo Mendoza-Azpur, Erik Valdivia, Yong-Han Koo, David M Kim
The goal of this investigation was to evaluate the bone-to-implant contact (BIC) of dental implants placed into fresh extraction sockets without pre-existing periapical pathology. When the extraction sites exhibited a gap distance of > 2 mm, autogenous bone harvested from surrounding surgical sites was grafted to fill that gap with no barrier membranes. All implants were clinically stable and successful at 6 months postoperative. The histologic examination demonstrated an average of 66.2% BIC for all five immediately placed dental implants...
March 2018: International Journal of Periodontics & Restorative Dentistry
Nima Madani, John S Kimball, Ashley P Ballantyne, David L R Affleck, Peter M van Bodegom, Peter B Reich, Jens Kattge, Anna Sala, Mona Nazeri, Matthew O Jones, Maosheng Zhao, Steven W Running
Plant traits are both responsive to local climate and strong predictors of primary productivity. We hypothesized that future climate change might promote a shift in global plant traits resulting in changes in Gross Primary Productivity (GPP). We characterized the relationship between key plant traits, namely Specific Leaf Area (SLA), height, and seed mass, and local climate and primary productivity. We found that by 2070, tropical and arid ecosystems will be more suitable for plants with relatively lower canopy height, SLA and seed mass, while far northern latitudes will favor woody and taller plants than at present...
February 12, 2018: Scientific Reports
Feng-Shan Gao, Xiao-Xin Zhai, Ping Jiang, Qiang Zhang, Hua Gao, Zi-Bin Li, Yong Han, Jie Yang, Zong-Hui Zhang
Currently available vaccines from inactivated Foot-and-mouth disease virus (FMDV) only protect animals by inducing neutralizing antibodies. A vaccine that contains cytotoxic T lymphocytes (CTL) epitopes to induce strong CTL responses might protect animals more effectively. Herein, we used swine leukocyte antigen class I (SLAI) proteins derived from six different strains of domestic pigs to screen and identify shared FMDV CTL epitopes. Four potential FMDV CTL epitopes (Q01, Q02, AS3, and QA4) were confirmed by mass spectrometry...
February 9, 2018: Gene
Yanxin An, Yan Song, Zhaoling Wang, Jing Wang, Gaoyi Wu, Guoxiong Zhu, Lei Chen
The use of low-intensity pulsed ultrasound (LIPUS) is a promising approach to promote osteogenesis. However, few studies have reported the influence of this technique on the osseointegration of endosseous implants, especially regarding different implant topographies. We focused on how the initial interaction between cells and the titanium surface is enhanced by LIPUS and the potential regulatory mechanisms. The bone marrow mesenchymal stem cells (BMSCs) of rats were cultured on two types of titanium surfaces (polished surface, Flat and large grain blast acid etched, SLA) under LIPUS stimulation or control conditions...
2018: American Journal of Translational Research
Yun Zou, Qing Han, Xisheng Weng, Yongwei Zou, Yingying Yang, Kesong Zhang, Kerong Yang, Xiaolin Xu, Chenyu Wang, Yanguo Qin, Jincheng Wang
Recently, clinical application of 3D printed model was increasing. However, there was no systemic study for confirming the precision and reliability of 3D printed model. Some senior clinical doctors mistrusted its reliability in clinical application. The purpose of this study was to evaluate the precision and reliability of stereolithography appearance (SLA) 3D printed model.Some related parameters were selected to research the reliability of SLA 3D printed model. The computed tomography (CT) data of bone/prosthesis and model were collected and 3D reconstructed...
February 2018: Medicine (Baltimore)
Dong He, Yongfa Chen, Kangning Zhao, J H C Cornelissen, Chengjin Chu
Background and Aims: How functional traits vary with environmental conditions is of fundamental importance in trait-based community ecology. However, how intraspecific variability in functional traits is connected to species distribution is not well understood. This study investigated inter- and intraspecific variation of a key functional trait, i.e. specific leaf area (leaf area per unit dry mass; SLA), in relation to soil factors and tested if trait variation is more closely associated with specific environmental regimes for low-variability species than for high-variability species...
February 3, 2018: Annals of Botany
Se Yeon Song, Min Soo Park, Jung Woo Lee, Ji Sun Yun
Silane coupling agents (SCAs) with different organofunctional groups were coated on the surfaces of Al₂O₃ ceramic particles through hydrolysis and condensation reactions, and the SCA-coated Al₂O₃ ceramic particles were dispersed in a commercial photopolymer based on interpenetrating networks (IPNs). The organofunctional groups that have high radical reactivity and are more effective in UV curing systems are usually functional groups based on acryl, such as acryloxy groups, methacrloxy groups, and acrylamide groups, and these silane coupling agents seem to improve interfacial adhesion and dispersion stability...
February 7, 2018: Nanomaterials
Vlatko Kastratović, Miljan Bigović, Željko Jaćimović, Milica Kosović, Dijana Đurović, Slađana Krivokapić
Macrophytes react to changes in the quality of the environment in which they live (water/sediment), and they are good bioindicators of surface water conditions. In the present study, the content of the metals cobalt (Co) and nickel (Ni) was determined in the sediment, the water, and different organs of macrophytes from six localities around Lake Skadar, across four different seasons of year. The aquatic macrophytes that have been used as bioindicator species in this study are Phragmites australis (an emerged species), Ceratophyllum demersum (a submerged species), and Lemna minor (a floating species)...
February 6, 2018: Environmental Science and Pollution Research International
Amaya Mendikoetxea, Cristóbal Lozano
This paper shows the need to triangulate different approaches in Bilingualism and Second Language Acquisition (SLA) research to fully understand late bilinguals' interlanguage grammars. Methodologically, we show how experimental and corpus data can be (and should be) triangulated by reporting on a corpus study (Lozano and Mendikoetxea in Biling Lang Cognit 13(4):475-497, 2010) and a new follow-up offline experiment investigating Subject-Verb inversion (Subject-Verb/Verb-Subject order) in L1 Spanish-L2 English (n = 417)...
February 5, 2018: Journal of Psycholinguistic Research
Ning Yang, Jiandong Li, Qian Yang, Jinzeng Qiao, Defeng Cui, Fenghua Liu, Huanrong Li, Shuanghai Zhou
The efficiency of immune responses and host defense against pathogens largely depends on the function of dendritic cells (DCs). Porcine circovirus type 2 (PCV2) infection causes viremia and extensive modulation of immune activities in the blood. The objective of the present study was to investigate the effects of PCV2 infection in vivo on the immunological function of DCs induced from peripheral blood monocytes (MoDCs). At different points after infection with PCV2, peripheral blood monocytes from PCV2-infected pigs were used to induce differentiation of DCs in vitro...
February 3, 2018: Archives of Virology
Xian Zhou, Xiulian Hu, Ye Lin
PURPOSE: The objective was to prepare tantalum (Ta)-coated sandblasted and acid-etched (SLA) dental implants using vacuum plasma spraying (VPS) and to analyze their morphologies. MATERIALS AND METHODS: Twelve SLA implants were coated with Ta using VPS. The topographies of the coatings and Ta/SLA surface interfaces were examined using scanning electron microscopy. The thickness at 4 locations for 6 Ta-coated and 6 uncoated SLA implants and pore sizes of the neck, central, and root areas of Ta-coated implants were measured...
January 30, 2018: Implant Dentistry
Masanobu Nakajima, Hiroyuki Kato, Hiroto Muroi, Maiko Kikuchi, Masakazu Takahashi, Satoru Yamaguchi, Kinro Sasaki, Hitoshi Ishikawa, Hideyuki Sakurai, Hiroyuki Kuwano
BACKGROUND/AIMS: Because salvage surgery after definitive chemoradiotherapy for esophageal cancer is associated with high postoperative mortality and morbidity, minimally invasive methods are desirable. We analyzed the validity of minimally invasive salvage operations (MISO). METHODS: Twenty-five patients underwent salvage operation between 2010 and 2016 in our institution, 10 having undergone right transthoracic salvage esophagectomy (TTSE group), 6 transhiatal salvage esophagectomy (THSE), 6 salvage lymphadenectomy (SLA), and 3 salvage endoscopic submucosal dissection (SESD)...
February 1, 2018: Digestion
(no author information available yet)
No abstract text is available yet for this article.
February 2018: Transplantation
Crystal E Owens, A John Hart
Wider use and adaptation of microfluidics is hindered by the infrastructure, knowledge, and time required to build prototype systems, especially when multiple fluid operations and measurements are required. As a result, 3D printing of microfluidics is attracting interest, yet cannot readily achieve the feature size, smoothness, and optical transparency needed for many standard microfluidic systems. Herein we present a new approach to the design and construction of high-precision modular microfluidics, using standard injection-molded blocks that are modified using micromilling and assembled via elastically averaged contacts...
January 26, 2018: Lab on a Chip
Daria Matczyńska, Daniel Sypniewski, Sabina Gałka, Dagna Sołtysik, Tomasz Loch, Ewa Nowak, Zdzisław Smorąg, Ilona Bednarek
Molecular characterization of the SLA (Swine Leukocyte Antigen) genes is important for understanding the immune responses between swine-donor and human-recipient in course of xenotransplantation. Explanation of association between alleles of SLA class I genes, type of pigs genetic modification, the PERV viral titer, and its subtypes may shed light on the nature of xenograft acceptance or rejection and the safety of xenotransplantation. No significant differences in PERV gag RNA level between transgenic and non-transgenic pigs were noted, likewise the type of applied transgene had no impact on PERV viremia...
January 25, 2018: Journal of Veterinary Science
Slađana Davidović, Miona Miljković, Miloš Tomić, Milan Gordić, Aleksandra Nešić, Suzana Dimitrijević
The aim of this study was to develop dextran-based edible films plasticized by sorbitol. In order to optimise the film-forming formulation, response surface methodology was used. The influence of dextran and sorbitol concentration on the mechanical and water vapour barrier properties of obtained films was investigated. The results showed that both parameters exhibited significant effect on the water vapour permeability of a film. Both dextran and sorbitol concentration had significant influence on tensile strength and elongation at break, whereas only sorbitol concentration had significant effect on Young's modulus...
March 15, 2018: Carbohydrate Polymers
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