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https://www.readbyqxmd.com/read/28823133/genetic-association-of-swine-leukocyte-antigen-class-ii-haplotypes-and-body-weight-in-microminipigs
#1
Tatsuya Matsubara, Masaki Takasu, Noriaki Imaeda, Naohito Nishii, Satoshi Takashima, Takashi Nishimura, Toshiaki Nishimura, Takashi Shiina, Asako Ando, Hitoshi Kitagawa
Objective: Microminipigs are a novel animal model with extensive applications in laboratory studies owing, in part, to their extremely small body sizes. In this study, the relationship between swine leukocyte antigen (SLA) class II haplotype and body weight was evaluated in the Microminipig population. Methods: A total of 1,900 haplotypes, covering SLA class II haplotypes Lr-0.7, Lr-0.23, Lr-0.17, Lr-0.37, Lr-0.16, Lr-0.11, Lr-0.13, and Lr-0.18, were analyzed in 950 piglets...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28814522/characterization-of-cis-acting-rna-elements-of-zika-virus-by-using-a-self-splicing-ribozyme-dependent-infectious-clone
#2
Zhong-Yu Liu, Jiu-Yang Yu, Xing-Yao Huang, Hang Fan, Xiao-Feng Li, Yong-Qiang Deng, Xue Ji, Meng-Li Cheng, Qing Ye, Hui Zhao, Jian-Feng Han, Xiao-Ping An, Tao Jiang, Bo Zhang, Yi-Gang Tong, Cheng-Feng Qin
Zika virus (ZIKV) has caused significant outbreaks and epidemics in the Americas recently, raising global concern due to its ability to cause microcephaly and other neurological complications. A stable and efficient infectious clone of ZIKV is urgently needed. However, the instability and toxicity of flavivirus cDNA clones in E. coli hosts has hindered the development of ZIKV infectious clones. Herein, using a novel self-splicing ribozyme-based strategy, we have generated a stable infectious cDNA clone of a contemporary ZIKV strain imported from Venezuela to China in 2016...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28810614/evolution-of-correlation-between-helicobacter-pylori-infection-and-autoimmune-liver-disease
#3
Xin-Guo Peng, Yu-Yuan Li, Hui-Ting Chen, Yan Zhou, Jian-Guo Ma, Hong-Min Yin
The present study was planned to evaluate correlation between Helicobacter pylori (HP) infection and autoimmune liver disease (AILD). A total of 60 patients diagnosed with AILD in Affiliated Hospital of Binzhou Medical College were continuously enrolled in the present study. HP infection was detected by 13C-urea breath test. The levels of anti-myeloperoxidase were tested by ELISA. The positive rate of anti-nuclear antibody (ANA), anti-mitochondrial antibody (AMA), anti-smooth muscle antibody (SMA) and anti-neutrophil cytoplasm antibody (ANCA) were tested by indirect immunofluorescence...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28783913/sea-level-anomaly-in-the-north-atlantic-and-seas-around-europe-long-term-variability-and-response-to-north-atlantic-teleconnection-patterns
#4
Isabel Iglesias, M Nieves Lorenzo, Clara Lázaro, M Joana Fernandes, Luísa Bastos
Sea level anomaly (SLA), provided globally by satellite altimetry, is considered a valuable proxy for detecting long-term changes of the global ocean, as well as short-term and annual variations. In this manuscript, monthly sea level anomaly grids for the period 1993-2013 are used to characterise the North Atlantic Ocean variability at inter-annual timescales and its response to the North Atlantic main patterns of atmospheric circulation variability (North Atlantic Oscillation, Eastern Atlantic, Eastern Atlantic/Western Russia, Scandinavian and Polar/Eurasia) and main driven factors as sea level pressure, sea surface temperature and wind fields...
August 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28781073/accuracy-of-stereolithography-additive-casts-used-in-a-digital-workflow
#5
Hiba Al-Imam, Mia Gram, Ana R Benetti, Klaus Gotfredsen
STATEMENT OF PROBLEM: Despite the increasing demand for a digital workflow in the fabrication of indirect restorations, information on the accuracy of the resulting definitive casts is limited. PURPOSE: The purpose of this in vitro study was to compare the accuracy of definitive casts produced with digital scans and conventional impressions. MATERIAL AND METHODS: Chamfer preparations were made on the maxillary right canine and second molar of a typodont...
August 4, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28757213/oxidative-stress-parameters-in-two-pelophylax-esculentus-complex-frogs-during-pre-and-post-hibernation-arousal-vs-heavy-metals
#6
Marko D Prokić, Slavica S Borković-Mitić, Imre I Krizmanić, Jelena J Mutić, Jelena P Gavrić, Svetlana G Despotović, Branka R Gavrilović, Tijana B Radovanović, Slađan Z Pavlović, Zorica S Saičić
In spring, frogs from temperate regions are faced with arousal-induced oxidative stress and exposure to various xenobiotics from the environment. The question is whether pollutants can significantly modify the antioxidative defense system (AOS) response of hibernators during recovery from hibernation. If this assumption is true, we would then expect different patterns of seasonal variations in the AOS between individuals exposed to different levels of pollution. To examine this assumption, we determined the relationship between seasonal variations of accumulated metals and AOS parameters in the skin and muscle of two frog species from the Pelophylax esculentus complex (P...
July 27, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28751109/polymorphism-and-peptide-binding-specificities-of-porcine-major-histocompatibility-complex-mhc-class-i-molecules
#7
Shuhua Fan, Yongli Wang, Song Wang, Xian Wang, Yanan Wu, Zibin Li, Nianzhi Zhang, Chun Xia
The swine lymphocyte antigen class I (SLA I) is a highly polymorphic gene superfamily that plays an important role in swine anti-viral immune responses. However, an understanding of the highly variable sites and peptide-binding specificities of SLA I molecule is limited. In this study, a total of 27 SLA I alleles were identified from 3 Tibetan wild boars and 3 Heishan pigs. The phylogenetic relationship between the Tibetan wild boar and other breeds was analyzed using bioinformatics methods, and the highly variable sites were noted in the three dimensional structures of SLA I...
July 24, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28740271/fabrication-of-a-3d-printing-definitive-obturator-prosthesis-a-clinical-report
#8
Theodoros Tasopoulos, Georgios Kouveliotis, Grigoris Polyzois, Vasiliki Karathanasi
INTRODUCTION: Digital technologies related to imaging and manufacturing provide the clinician with a wide variety of treatment options. Stereolithography (SLA) offers a simple and predictable way for an accurate reconstruction of congenital or acquired defects. CLINICAL CASE: A 65-years old cancer patient with non- keratinized squamous cell carcinoma of left maxillary sinus came for a prosthetic clinical evaluation. A bilateral maxillectomy was performed and the treatment plan included definite obturator prosthesis for the upper arch...
March 2017: Acta Stomatologica Croatica
https://www.readbyqxmd.com/read/28731897/marginal-bone-loss-around-early-loaded-sla-and-slactive-implants-radiological-follow-up-evaluation-up-to-6-5-years
#9
Işil Damla Şener-Yamaner, Gökhan Yamaner, Atilla Sertgöz, Cenk Fatih Çanakçi, Mutlu Özcan
PURPOSE: The aim of this study was to compare marginal bone loss around early-loaded SLA and SLActive tissue-level implants (Straumann Dental Implants; Institut Straumann AG, Basel, Switzerland) after a mean of 81-month follow-up period. MATERIAL AND METHODS: One hundred seven SLA and 68 SLActive implants were placed in 55 patients and loaded with final restoration after 8 and 3 weeks of healing time, respectively. Marginal bone loss around implants was determined radiographically at initial and after a mean observation time ranging between 20 and 81 months...
August 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28728120/cadmium-and-high-temperature-effects-on-brain-and-behaviour-of-lymantria-dispar-l-caterpillars-originating-from-polluted-and-less-polluted-forests
#10
Vesna Perić-Mataruga, Branka Petković, Larisa Ilijin, Marija Mrdaković, Slađana Dronjak Čučaković, Dajana Todorović, Milena Vlahović
Insects brain as a part of nervous system is the first-line of fast stress response that integrate stress signals to regulate all aspects of insect physiology and behaviour. The cadmium (Cd) bioaccumulation factor (BF), activity of the neurotoxicity biomarker acetylcholinesterase (AChE), dopamine content, expression and amount of Hsp70 in the brain and locomotor activity were evaluated in the 4th instar of Lymantria dispar L. caterpillars fed a Cd supplemented diet and reared in an optimal temperature regime (23 °C) and/or exposed to high temperature (28 °C)...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28726927/custom-3d-printer-and-resin-for-18-%C3%AE-m-%C3%A3-20-%C3%AE-m-microfluidic-flow-channels
#11
Hua Gong, Bryce P Bickham, Adam T Woolley, Gregory P Nordin
While there is great interest in 3D printing for microfluidic device fabrication, to-date the achieved feature sizes have not been in the truly microfluidic regime (<100 μm). In this paper we demonstrate that a custom digital light processor stereolithographic (DLP-SLA) 3D printer and a specifically-designed, low cost, custom resin can readily achieve flow channel cross sections as small as 18 μm × 20 μm. Our 3D printer has a projected image plane resolution of 7.6 μm and uses a 385 nm LED, which dramatically increases the available selection of UV absorbers for resin formulation compared to 3D printers with 405 nm LEDs...
July 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28717801/mimicking-arterial-thrombosis-in-a-3d-printed-microfluidic-in-vitro-vascular-model-based-on-computed-tomography-angiography-data
#12
Pedro F Costa, Hugo J Albers, John E A Linssen, Heleen H T Middelkamp, Linda van der Hout, Robert Passier, Albert van den Berg, Jos Malda, Andries D van der Meer
Arterial thrombosis is the main instigating factor of heart attacks and strokes, which result in over 14 million deaths worldwide every year. The mechanism of thrombosis involves factors from the blood and the vessel wall, and it also relies strongly on 3D vessel geometry and local blood flow patterns. Microfluidic chip-based vascular models allow controlled in vitro studies of the interaction between vessel wall and blood in thrombosis, but until now, they could not fully recapitulate the 3D geometry and blood flow patterns of real-life healthy or diseased arteries...
August 8, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28715926/microencapsulation-of-anthocyanin-rich-black-soybean-coat-extract-by-spray-drying-using-maltodextrin-gum-arabic-and-skimmed-milk-powder
#13
Ana Kalušević, Steva Lević, Bojan Čalija, Milena Pantić, Miona Belović, Vladimir Pavlović, Branko Bugarski, Jela Milić, Slađana Žilić, Viktor Nedović
Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80...
August 2, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28708908/activation-of-osteoblastic-function-on-titanium-surface-with-titanium-doped-hydroxyapatite-nanoparticle-coating-an-in-vitro-study
#14
Masahiro Nakazawa, Masahiro Yamada, Masato Wakamura, Hiroshi Egusa, Kaoru Sakurai
PURPOSE: Titanium-doped hydroxyapatite (TiHA) nanoparticles contain titanium atoms in the hydroxyapatite lattice, which can physicochemically functionalize the titanium surface without modification of the surface topography. This study aimed to evaluate the physicochemical properties of machined or microroughened titanium surfaces coated with TiHA nanoparticles and the functions of osteoblasts cultured on them. MATERIALS AND METHODS: Titanium disks with commercially available surface topography, such as machined or sandblasted, large-grit, and acid-etched (SLA) surfaces, were coated with TiHA...
July 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28707226/the-role-of-macrophage-polarization-on-fibroblast-behavior-an-in-vitro-investigation-on-titanium-surfaces
#15
Xuzhu Wang, Yulan Wang, Dieter D Bosshardt, Richard J Miron, Yufeng Zhang
OBJECTIVES: This study investigated the effect of smooth and rough titanium surface topographies on macrophage polarization and their influence on gingival fibroblast behavior cultured on titanium surfaces. MATERIALS AND METHODS: RAW 264.7 macrophages were seeded on smooth (pickled titanium (PT)) and rough Sand-blasted with Large grit particles followed by Acid-etching (SLA) titanium surfaces and first investigated for macrophage polarization towards tissue-inflammatory M1 macrophages or wound-healing M2 macrophages...
July 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28690845/diagnostic-autoantibodies-for-autoimmune-liver-diseases
#16
REVIEW
Ban-Hock Toh
Autoimmune liver diseases are conditions of low prevalence that comprise the triad of autoimmune hepatitis, primary biliary cholangitis (cirrhosis) and primary sclerosing cholangitis and their poorly characterised overlapping syndromes. Diagnostic autoantibodies are associated with autoimmune hepatitis and primary biliary cholangitis but not with primary sclerosing cholangitis. Autoantibodies are useful disease markers that facilitate early diagnosis of autoimmune hepatitis and primary biliary cholangitis and allow for therapeutic intervention to prevent progression to liver cirrhosis and associated complications...
May 2017: Clinical & Translational Immunology
https://www.readbyqxmd.com/read/28680714/characteristics-of-contact-and-distance-osteogenesis-around-modified-implant-surfaces-in-rabbit-tibiae
#17
Jung-Yoo Choi, Jae-Hyuk Sim, In-Sung Luke Yeo
PURPOSE: Contact and distance osteogenesis occur around all endosseous dental implants. However, the mechanisms underlying these processes have not been fully elucidated. We hypothesized that these processes occur independently of each other. To test this, we used titanium (Ti) tubes to physically separate contact and distance osteogenesis, thus allowing contact osteogenesis to be measured in the absence of possible triggers from distance osteogenesis. METHODS: Sandblasted and acid-etched (SLA) and modified SLA (modSLA) implants were used...
June 2017: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/28680548/evaluation-of-marginal-and-internal-gaps-of-ni-cr-and-co-cr-alloy-copings-manufactured-by-microstereolithography
#18
Dong-Yeon Kim, Chong-Myeong Kim, Ji-Hwan Kim, Hae-Young Kim, Woong-Chul Kim
PURPOSE: The purpose of this study was to evaluate the marginal and internal gaps of Ni-Cr and Co-Cr copings, fabricated using the dental µ-SLA system. MATERIALS AND METHODS: Ten study dies were made using a two-step silicone impression with a dental stone (type IV) from the master die of a tooth. Ni-Cr (NC group) and Co-Cr (CC group) alloy copings were designed using a dental scanner, CAD software, resin coping, and casting process. In addition, 10 Ni-Cr alloy copings were manufactured using the lost-wax technique (LW group)...
June 2017: Journal of Advanced Prosthodontics
https://www.readbyqxmd.com/read/28670315/sequence-based-genotyping-of-expressed-swine-leukocyte-antigen-class-i-alleles-by-next-generation-sequencing-reveal-novel-swine-leukocyte-antigen-class-i-haplotypes-and-alleles-in-belgian-danish-and-kenyan-fattening-pigs-and-g%C3%A3-ttingen-minipigs
#19
Maria Rathmann Sørensen, Mette Ilsøe, Mikael Lenz Strube, Richard Bishop, Gitte Erbs, Sofie Bruun Hartmann, Gregers Jungersen
The need for typing of the swine leukocyte antigen (SLA) is increasing with the expanded use of pigs as models for human diseases and organ-transplantation experiments, their use in infection studies, and for design of veterinary vaccines. Knowledge of SLA sequences is furthermore a prerequisite for the prediction of epitope binding in pigs. The low number of known SLA class I alleles and the limited knowledge of their prevalence in different pig breeds emphasizes the need for efficient SLA typing methods. This study utilizes an SLA class I-typing method based on next-generation sequencing of barcoded PCR amplicons...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28667473/differences-in-functional-traits-between-invasive-and-native-amaranthus-species-under-different-forms-of-n-deposition
#20
Congyan Wang, Jiawei Zhou, Jun Liu, Kun Jiang
Differences in functional traits between invasive and native plant species are believed to determine the invasion success of the former. Increasing amounts of anthropogenic nitrogen (N) are continually deposited into natural ecosystems, which may change the relative occurrence of the different N deposition forms (such as NH4-N, NO3-N, and CO(NH2)2-N) naturally deposited. Under high N deposition scenarios, some invasive species may grow faster, gaining advantage over native species. In a greenhouse experiment, we grew invasive and native Amaranthus species from seed both alone and in competition under simulated N enriched environments with different forms of N over 3 months...
August 2017: Die Naturwissenschaften
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