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https://www.readbyqxmd.com/read/29792521/problematic-video-gaming-in-a-young-spanish-population-association-with-psychosocial-health
#1
Carlos Buiza-Aguado, Araceli Alonso-Canovas, Celia Conde-Mateos, Juan Jose Buiza-Navarrete, Douglas Gentile
Problematic video gaming (PVG) is a concern for psychologists attending children and adolescents. Uniform diagnostic criteria are lacking, and risk factors are poorly understood. Internet gaming disorder (IGD) was included in the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5), and scales derived from its diagnostic criteria may be helpful to assess PVG. A multicenter study was conducted in secondary schools using an IGD-derived scale (dichotomous Nine-Item Internet Gaming Disorder Scale [IGD-9]), analyzing PVG-related variables...
May 24, 2018: Cyberpsychology, Behavior and Social Networking
https://www.readbyqxmd.com/read/29792492/evaluation-of-second-step-on-early-elementary-students-academic-outcomes-a-randomized-controlled-trial
#2
Clayton R Cook, Sabina Low, Joanne Buntain-Ricklefs, Kelly Whitaker, Michael D Pullmann, Jaclyn Lally
Research has consistently linked social-emotional learning to important educational and life outcomes. Early elementary represents an opportune developmental period to proactively support children to acquire social-emotional skills that enable academic success. Using data from a large scale randomized controlled trial, the purpose of this study was to investigate the impact of the 4th edition of Second Step on early elementary students' academic-related outcomes. Participants were Kindergarten to 2nd grade students in 61 schools (310 teachers; 7,419 students) across six school districts in Washington State and Arizona...
May 24, 2018: School Psychology Quarterly
https://www.readbyqxmd.com/read/29792239/screening-for-delirium-with-the-intensive-care-delirium-screening-checklist-icdsc-symptom-profile-and-utility-of-individual-items-in-the-identification-of-delirium-dependent-on-the-level-of-sedation
#3
Soenke Boettger, Rafael Meyer, André Richter, Susana Franco Fernandez, Alain Rudiger, Maria Schubert, Josef Jenewein, David Garcia Nuñez
OBJECTIVE: The importance of the proper identification of delirium, with its high incidence and adversities in the intensive care setting, has been widely recognized. One common screening instrument is the Intensive Care Delirium Screening Checklist (ICDSC); however, the symptom profile and key features of delirium dependent on the level of sedation have not yet been evaluated. METHOD: In this prospective cohort study, the ICDSC was evaluated versus the Diagnostic and Statistical Manual, 4th edition, text revision, diagnosis of delirium set as standard with respect to the symptom profile, and correct identification of delirium...
May 24, 2018: Palliative & Supportive Care
https://www.readbyqxmd.com/read/29791966/outcomes-of-bethesda-categories-iii-and-iv-thyroid-nodules-over-5-years-and-performance-of-the-afirma-gene-expression-classifier-a-single-institution-study
#4
Kelsi E Deaver, Bryan R Haugen, Nikita Pozdeyev, Carrie B Marshall
OBJECTIVE: The second edition Bethesda System for Reporting Thyroid Cytology estimates 6-18% malignancy rate of category III (B3) and 10-40% for category IV (B4) nodules; however, reported malignancy rates have considerable variability among institutions. Use of molecular classifiers (including Afirma Gene Expression Classifier, GEC) can be utilized in management of thyroid nodules. Our objective was to analyze malignancy rates of B3 and B4 nodules and determine clinical outcomes of GEC Benign nodules...
May 23, 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/29791835/nicotinic-cholinergic-receptors-in-vta-glutamate-neurons-modulate-excitatory-transmission
#5
Yijin Yan, Can Peng, Matthew C Arvin, Xiao-Tao Jin, Veronica J Kim, Matthew D Ramsey, Yong Wang, Sambashiva Banala, David L Wokosin, J Michael McIntosh, Luke D Lavis, Ryan M Drenan
Ventral tegmental area (VTA) glutamate neurons are important components of reward circuitry, but whether they are subject to cholinergic modulation is unknown. To study this, we used molecular, physiological, and photostimulation techniques to examine nicotinic acetylcholine receptors (nAChRs) in VTA glutamate neurons. Cells in the medial VTA, where glutamate neurons are enriched, are responsive to acetylcholine (ACh) released from cholinergic axons. VTA VGLUT2+ neurons express mRNA and protein subunits known to comprise heteromeric nAChRs...
May 22, 2018: Cell Reports
https://www.readbyqxmd.com/read/29790644/crispr-cas9-assisted-multiplex-genome-editing-technique-in-escherichia-coli
#6
Xu Feng, Dongdong Zhao, Xueli Zhang, Xiang Ding, Changhao Bi
Genome editing for site-specific chromosome modification is one of the most significant techniques in biological research. While conventional techniques usually deal with one genomic locus at a time, multiple genomic targets are often required to be modified to develop microbial cell factories. Thus, it is necessary to develop techniques for simultaneous editing of multiple loci. In this work, we developed a CRISPR/Cas9 assisted multiplex genome editing (CMGE) technique in Escherichia coli. With this editing method, all functional parts were assembled into replicable plasmids, and stringent inducible expression systems were used to control Cas9 gene expression, which was to decouple transformation from editing process to increase editing efficiency...
May 23, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29790357/-control-editing-of-the-genome-within-reach-or-already-in-our-hands
#7
Radim Brdička, Radoslav Omelka
Although different genome editing tools have been around for decades, the recent emergence of cheap, quick, and accessible CRISPR/Cas9 technology has led to a revolution in this field. The technique has the potential to transform medicine from curative into preventive using a gene therapy. An application of genome editing has proven to be effective for both genetic and non-genetic (e.g. infectious) diseases. However, cancer and rare diseases treatment is at the forefront of interest. Concurrently, the legal and ethical frameworks should be discussed, especially as the technology moves towards a modification of the germ cells or embryos...
2018: Casopís Lékar̆ů C̆eských
https://www.readbyqxmd.com/read/29790280/cas9-versus-cas12a-cpf1-structure-function-comparisons-and-implications-for-genome-editing
#8
REVIEW
Daan C Swarts, Martin Jinek
Cas9 and Cas12a are multidomain CRISPR-associated nucleases that can be programmed with a guide RNA to bind and cleave complementary DNA targets. The guide RNA sequence can be varied, making these effector enzymes versatile tools for genome editing and gene regulation applications. While Cas9 is currently the best-characterized and most widely used nuclease for such purposes, Cas12a (previously named Cpf1) has recently emerged as an alternative for Cas9. Cas9 and Cas12a have distinct evolutionary origins and exhibit different structural architectures, resulting in distinct molecular mechanisms...
May 22, 2018: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/29789579/endogenous-authentic-oct4a-proteins-directly-regulate-fos-ap-1-transcription-in-somatic-cancer-cells
#9
Yanwen Zhou, Xinyu Chen, Bo Kang, Shiqi She, Xiaobing Zhang, Cheng Chen, Wenxin Li, Wenjie Chen, Songsong Dan, Xiaoyun Pan, Xiaoli Liu, Jianqin He, Qingwei Zhao, Chenggang Zhu, Ling Peng, Haoyi Wang, Hangping Yao, Hongcui Cao, Lanjuan Li, Meenhard Herlyn, Ying-Jie Wang
OCT4A is well established as a master transcription factor for pluripotent stem cell (PSC) self-renewal and a pioneer factor for initiating somatic cell reprogramming, yet its presence and functionality in somatic cancer cells remain controversial and obscure. By combining the CRISPR-Cas9-based gene editing with highly specific PCR assays, highly sensitive immunoassays, and mass spectrometry, we provide unequivocal evidence here that full-length authentic OCT4A transcripts and proteins were both present in somatic cancer cells, and OCT4A proteins were heterogeneously expressed in the whole cell population and when expressed, they are predominantly localized in cell nucleus...
May 22, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29789521/triad3a-induces-the-degradation-of-early-necrosome-to-limit-ripk1-dependent-cytokine-production-and-necroptosis
#10
Norah A Alturki, Scott McComb, Ardeshir Ariana, Dikchha Rijal, Robert G Korneluk, Shao-Cong Sun, Emad Alnemri, Subash Sad
Understanding the molecular signaling in programmed cell death is vital to a practical understanding of inflammation and immune cell function. Here we identify a previously unrecognized mechanism that functions to downregulate the necrosome, a central signaling complex involved in inflammation and necroptosis. We show that RipK1 associates with RipK3 in an early necrosome, independent of RipK3 phosphorylation and MLKL-induced necroptotic death. We find that formation of the early necrosome activates K48-ubiquitin-dependent proteasomal degradation of RipK1, Caspase-8, and other necrosomal proteins...
May 22, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29789427/impact-of-tobacco-specific-nitrosamine-derived-dna-adducts-on-the-efficiency-and-fidelity-of-dna-replication-in-human-cells
#11
Hua Du, Jiapeng Leng, Pengcheng Wang, Lin Li, Yinsheng Wang
The tobacco-derived nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N '-nitrosonornicotine (NNN) are known human carcinogens. Following metabolic activation, NNK and NNN can induce a number of DNA lesions, including several 4-(3-pyridyl)-4-oxobut-1-yl (POB) adducts. However, it remains unclear to what extent these lesions affect the efficiency and accuracy of DNA replication and how their replicative bypass is influenced by translesion synthesis (TLS) DNA polymerases. In this study, we investigated the effects of three stable POB DNA adducts (i...
May 22, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29789357/reactivation-of-%C3%AE-globin-in-adult-%C3%AE-yac-mice-after-ex-vivo-and-in-vivo-hematopoietic-stem-cell-genome-editing
#12
Chang Li, Nikoletta Psatha, Pavel Sova, Sucheol Gil, Hongjie Wang, Jiho Kim, Chandana Kulkarni, Cristina Valensisi, R David Hawkins, George Stamatoyannopoulos, André Lieber
Disorders involving β-globin gene mutations, mainly β-thalassemia and sickle cell disease, represent a major target for hematopoietic stem/progenitor cell (HSPC) gene therapy. This includes CRISPR/Cas9-mediated genome editing approaches in adult CD34+ cells aimed toward the re-activation of fetal γ-globin expression in red blood cells. Because models involving erythroid differentiation of CD34+ cells have limitations in assessing γ-globin re-activation, we focused on human β-globin locus transgenic (β-YAC) mice...
May 22, 2018: Blood
https://www.readbyqxmd.com/read/29789263/gross-motor-skills-in-toddlers-prevalence-and-socio-demographic-differences
#13
Sanne L C Veldman, Rachel A Jones, Rute Santos, Eduarda Sousa-Sá, Anthony D Okely
OBJECTIVES: Gross motor skills (GMS) are a vital component of a child's development. Monitoring levels and correlates of GMS is important to ensure appropriate strategies are put in place to promote these skills in young children. The aim of this study was to describe the current level of GMS development of children aged 11-29months and how these levels differ by age, sex, BMI and socio-economic status. DESIGN: Cross-sectional study. METHODS: This study involved children from 30 childcare services in NSW, Australia...
May 19, 2018: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/29789195/steroid-pulse-therapy-transiently-destroys-the-discriminative-histological-structure-of-tonsils-in-iga-nephropathy-tonsillectomy-should-be-performed-before-or-just-after-steroid-pulse-therapy
#14
Mika Adachi, Mitsuhiro Sato, Mariko Miyazaki, Osamu Hotta, Koji Hozawa, Toshinobu Sato, Yoshio Taguma, Yukio Katori
OBJECTIVE: Tonsillectomy combined with steroid-pulse therapy is a widely accepted method for the treatment of IgA nephropathy (IgAN) in Japan. However, the indication of tonsillectomy for IgAN is still controversial, and the timing of tonsillectomy is not clearly defined for the protocol of this therapy. Based on the results of a randomized control trial in Japan, the Evidence-Based Clinical Practice Guidelines for IgA nephropathy 2014 (edited in Japan) recommended tonsillectomy combined with steroid-pulse therapy for Grade C1...
May 19, 2018: Auris, Nasus, Larynx
https://www.readbyqxmd.com/read/29788858/capacity-of-objective-measures-of-physical-activity-to-predict-brazilian-children-s-low-motor-proficiency
#15
Manoella O Santos, Diego G Barbosa, Geraldo J F Junior, Raísa C Silva, Andreia Pelegrini, Érico P G Felden
This study analyzed the associations between physical activity (PA) and motor proficiency (MP) so as to determine what level of moderate PA might help avoid low MP in 8-10-year-old Brazilian children. We assessed MP of 98 children using the Bruininks-Oseretsky Motor Proficiency, Second Edition Short Form (BOT-2 SF), and we assessed PA using the GT3X + accelerometer. We analyzed data using means, standard deviations, frequency distribution, spearman coefficient correlation test, Mann-Whitney U test, and receiver operating characteristics curve...
January 1, 2018: Perceptual and Motor Skills
https://www.readbyqxmd.com/read/29788753/do-gaming-disorder-and-hazardous-gaming-belong-in-the-icd-11-considerations-regarding-the-death-of-a-hospitalized-patient-that-was-reported-to-have-occurred-while-a-care-provider-was-gaming
#16
Marc N Potenza
There has been much debate regarding the extent to which different types and patterns of gaming may be considered harmful from individual and public health perspectives. A recent event in which a hospitalized patient was reported to have died while a care provider was gaming is worth considering as an example as to how gaming may distract individuals from work-related tasks or other activities, with potential negative consequences. As the 11th edition of the International Classification of Diseases is being developed, events like these are important to remember when considering entities like, and generating criteria for, disordered or hazardous gaming...
May 23, 2018: Journal of Behavioral Addictions
https://www.readbyqxmd.com/read/29788529/-a-web-based-colour-vision-test-as-a-tool-for-qualitative-evaluation-of-pseudoisochromatic-pfl%C3%A3-ger-trident-colour-plates
#17
Joern Kuchenbecker
BACKGROUND: Pseudoisochromatic colour plates are constructed according to specific principles. They can be very different in quality. To check the diagnostic quality, they have to be tested on a large number of subjects, but this procedure is can be tedious and expensive. Therefore, the use of a standardised web-based test is recommended. METHODS: Eight Pflüger trident colour plates (including 1 demo plate) according to the Velhagen edition of 1980 were digitised and inserted into a web-based colour vision test (www...
May 22, 2018: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/29788381/the-role-of-apathy-and-depression-on-verbal-learning-and-memory-performance-after-stroke
#18
Keera N Fishman, Andrea R Ashbaugh, Krista L Lanctôt, Megan L Cayley, Nathan Herrmann, Brian J Murray, Michelle Sicard, Karen Lien, Demetrios J Sahlas, Richard H Swartz
Objective: Psychiatric symptoms, including depression and apathy, may significantly impede functional and cognitive capabilities following a cerebrovascular event. This study examined the role of apathy and depression on learning and memory performance in stroke patients. Method: Stroke patients (n = 140 [119 ischemic, 21 hemorrhagic], mean age = 60.6 [SD = 15.1]) completed the Apathy Evaluation Scale (AES), the Center for Epidemiologic Studies Depression Scale (CES-D), and the California Verbal Learning Test-Second Edition (CVLT-II)...
May 18, 2018: Archives of Clinical Neuropsychology: the Official Journal of the National Academy of Neuropsychologists
https://www.readbyqxmd.com/read/29788374/presence-of-hpv-apobec-related-minority-variants-in-hpv16-genomes-from-anal-and-cervical-samples-but-not-in-hpv52-and-hpv58
#19
Alice-Andrée Mariaggi, Hélène Péré, Marine Perrier, Benoit Visseaux, Gilles Collin, David Veyer, Quentin Le Hingrat, Valentine Marie Ferré, Véronique Joly, Anne Couvelard, Margot Bucau, Carine Davitian, Diane Descamps, Laurent Abramowitz, Charlotte Charpentier
Background: The aim of this study was to investigate the presence of minority variants (MV) in high-risk Human Papillomavirus types (hrHPV16, HPV52, HPV58) from cervical and anal smears. Methods: Whole HPV genome ultra-deep sequencing (UDS) was performed on cervical and anal smears collected during patients' follow-up. Bioinformatics analyses were performed using Bowtie2® (Geneious®). Results: We assessed 55 HPV16+, 20 HPV52+ and 17 HPV58+ samples, with significant differences in patient characteristics for the two anatomic sites...
May 17, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29788086/prognostic-ability-of-new-t1-descriptors-in-the-tumour-node-and-metastasis-classification-of-surgically-treated-non-small-cell-lung-cancer
#20
Tomoyoshi Takenaka, Koji Yamazaki, Naoko Miura, Sadanori Takeo
OBJECTIVES: In the tumour, node and metastasis (TNM) classification (8th edition) of non-small-cell lung cancer, T (tumour size) is determined solely according to the size of the solid component determined using computed tomography (CT). However, it is unclear whether tumours of equal size but with differing solid and part-solid components should be similarly treated. Herein, we assessed the prognostic significance of the newly proposed T1 descriptors with respect to the size of the solid component...
May 19, 2018: Interactive Cardiovascular and Thoracic Surgery
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