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https://www.readbyqxmd.com/read/28510253/feline-immunodeficiency-virus-and-feline-leukemia-virus-frequency-and-associated-factors-in-cats-in-northeastern-brazil
#1
L C Lacerda, A N Silva, J S Freitas, R D S Cruz, R A Said, A D Munhoz
Our aims were to determine the frequencies of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in owned and stray cats in the northeastern region of Brazil, ascertain the status of FeLV infection, and investigate potential associated factors among the owned cats. Blood samples from 200 asymptomatic owned cats and 30 stray cats were processed using nested PCR and commercial immunochromatographic tests to diagnose infections. To evaluate the factors associated with FIV and/or FeLV in owned cats, a semi-structured interview was conducted with each owner about the animal's environment, and these data were subjected to unconditional logistic regression...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28491447/successful-treatment-of-multifocal-pedal-prototheca-wickerhamii-infection-in-a-feline-immunodeficiency-virus-positive-cat-with-multiple-bowenoid-in-situ-carcinomas-containing-papillomaviral-dna-sequences
#2
Allan E Kessell, Derek McNair, John S Munday, Richard Savory, Catriona Halliday, Richard Malik
CASE SUMMARY: A 16-year-old, castrated male, feline immunodeficiency virus (FIV)-positive, domestic shorthair cat developed multiple skin lesions. Most of these were Bowenoid carcinoma in situ and contained DNA sequences consistent with Felis catus papillomavirus type 2. Two additional lesions that developed in the skin and subcutaneous tissues between the digital and carpal pads on the left forelimb and right hindlimb were shown by cytology, histology and culture to be caused by Prototheca wickerhamii...
January 2017: JFMS Open Rep
https://www.readbyqxmd.com/read/28491433/evaluation-of-rapid-diagnostic-test-kits-for-feline-leukemia-virus-infection-using-samples-from-naturally-infected-cats
#3
Jiayou Liu, Thomas O'Connor, Melissa Beall, Ramaswamy Chandrashekar, Michael Lappin
OBJECTIVES: Feline leukemia virus (FeLV) is a potentially life-threatening oncogenic retrovirus. The p27 viral core protein is produced by the virus in infected feline cells, is found in the cytoplasm in several blood cells and can be free in the serum and plasma. ELISA or particle-based immunoassay are commonly used to detect the presence of the p27 core protein in samples obtained from blood. The objective of this study was to compare the performance of several in-clinic tests: the SNAP Feline Triple Test (IDEXX Laboratories), the WITNESS FeLV-FIV Test (Zoetis) and the VetScan Feline FeLV/FIV Rapid Test (Abaxis)...
July 2016: JFMS Open Rep
https://www.readbyqxmd.com/read/28475623/analysis-of-the-functional-compatibility-of-siv-capsid-sequences-in-the-context-of-the-fiv-gag-precursor
#4
César A Ovejero, José L Affranchino, Silvia A González
The formation of immature lentiviral particles is dependent on the multimerization of the Gag polyprotein at the plasma membrane of the infected cells. One key player in the virus assembly process is the capsid (CA) domain of Gag, which establishes the protein-protein interactions that give rise to the hexagonal lattice of Gag molecules in the immature virion. To gain a better understanding of the functional equivalence between the CA proteins of simian and feline immunodeficiency viruses (SIV and FIV, respectively), we generated a series of chimeric FIV Gag proteins in which the CA-coding region was partially or totally replaced by its SIV counterpart...
2017: PloS One
https://www.readbyqxmd.com/read/28384338/peripheral-and-central-immune-cell-reservoirs-in-tissues-from-asymptomatic-cats-chronically-infected-with-feline-immunodeficiency-virus
#5
C D Eckstrand, E E Sparger, K A Pitt, B G Murphy
Feline immunodeficiency virus (FIV) infection in cats results in life-long viral persistence and progressive immunopathology. We have previously described a cohort of experimentally infected cats demonstrating a progressive decline of peripheral blood CD4+ T-cell over six years in the face of apparent peripheral viral latency. More recently we reported findings from this same cohort that revealed popliteal lymph node tissue as sites for ongoing viral replication suggesting that tissue reservoirs are important in FIV immunopathogenesis during the late asymptomatic phase of infection...
2017: PloS One
https://www.readbyqxmd.com/read/28375041/prevalence-of-external-ear-disorders-in-belgian-stray-cats
#6
Anouck Bollez, Hilde de Rooster, Alessandra Furcas, Sophie Vandenabeele
Objectives Feline otitis externa is a multifactorial dermatological disorder about which very little is known. The objective of this study was to map the prevalence of external ear canal disorders and the pathogens causing otitis externa in stray cats roaming around the region of Ghent, Belgium. Methods One hundred and thirty stray cats were randomly selected during a local trap-neuter-return programme. All cats were European Shorthairs. This study included clinical, otoscopic and cytological evaluation of both external ears of each cat...
April 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28360895/genetic-diversity-of-brucella-reference-and-non-reference-phages-and-its-impact-on-brucella-typing
#7
Jens A Hammerl, Cornelia Göllner, Claudia Jäckel, Holger C Scholz, Karsten Nöckler, Jochen Reetz, Sascha Al Dahouk, Stefan Hertwig
Virulent phages have been used for many years to type Brucella isolates, but until recently knowledge about the genetic makeup of these phages remains limited. In this work the host specificity and genomic sequences of the original set (deposited in 1960) of VLA Brucella reference phages Tb, Fi, Wb, Bk2, R/C, and Iz were analyzed and compared with hitherto described brucellaphages. VLA phages turned out to be different from homonymous phages in other laboratories. The host range of the phages was defined by performing plaque assays with a wide selection of Brucella strains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28358636/seroprevalence-of-heartworm-infection-risk-factors-for-seropositivity-and-frequency-of-prescribing-heartworm-preventives-for-cats-in-the-united-states-and-canada
#8
Julie K Levy, Amie N Burling, Michael M Crandall, Sylvia J Tucker, Erin G Wood, Jessie D Foster
OBJECTIVE To determine the seroprevalence of heartworm infection, risk factors for seropositivity, and frequency of prescribing heartworm preventives for cats. DESIGN Prospective cross-sectional study. ANIMALS 34,975 cats from 1,353 veterinary clinics (n = 26,707) and 125 animal shelters (8,268) in the United States and Canada. PROCEDURES Blood samples were collected from all cats and tested with a point-of-care ELISA for Dirofilaria immitis antigen, FeLV antigen, and FIV antibody. Results were compared among geographic regions and various cat groupings...
April 15, 2017: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/28331087/feline-immunodeficiency-virus-evolutionarily-acquires-two-proteins-vif-and-protease-capable-of-antagonizing-feline-apobec3
#9
Rokusuke Yoshikawa, Junko S Takeuchi, Eri Yamada, Yusuke Nakano, Naoko Misawa, Yuichi Kimura, Fengrong Ren, Takayuki Miyazawa, Yoshio Koyanagi, Kei Sato
The interplay between viral and host proteins has been well studied to elucidate virus-host interactions and their relevance to virulence. Mammalian genes encode apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) proteins, which act as intrinsic restriction factors against lentiviruses. To overcome APOBEC3-mediated anti-viral actions, lentiviruses have evolutionarily acquired an accessory protein, viral infectivity factor (Vif), and Vif degrades host APOBEC3 proteins via a ubiquitin/proteasome-dependent pathway...
March 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28285597/prevalence-study-and-risk-factor-analysis-of-selected-bacterial-protozoal-and-viral-including-vector-borne-pathogens-in-cats-from-cyprus
#10
Charalampos Attipa, Kostas Papasouliotis, Laia Solano-Gallego, Gad Baneth, Yaarit Nachum-Biala, Elpida Sarvani, Toby G Knowles, Sena Mengi, David Morris, Chris Helps, Séverine Tasker
BACKGROUND: Feline infectious agent studies are lacking in Cyprus. The aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (FVBP), in cats from Cyprus. METHODS: A cross-sectional, descriptive, multicentre study was performed on 174 feline samples [138 owned and 36 shelter-feral, including both healthy (43) and non-healthy (131), cats] from private veterinary clinics from all six districts of Cyprus...
March 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28285590/molecular-detection-of-hepatozoon-spp-and-cytauxzoon-sp-in-domestic-and-stray-cats-from-madrid-spain
#11
David Díaz-Regañón, Alejandra Villaescusa, Tania Ayllón, Fernando Rodríguez-Franco, Gad Baneth, Lydia Calleja-Bueno, Mercedes García-Sancho, Beatriz Agulla, Ángel Sainz
BACKGROUND: Different species of apicomplexan protozoans of the genera Hepatozoon and Cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. The aim of this study was to assess the molecular prevalence of Hepatozoon spp. and Cytauxzoon spp. and to identify associated risk factors and clinical and laboratory abnormalities in a population of cats from Madrid, Spain. METHODS: Six hundred and forty-four client-owned and stray cats from Madrid, Spain, were included in this study...
March 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28285588/prevalence-and-molecular-characterization-of-canine-and-feline-hemotropic-mycoplasmas-hemoplasmas-in-northern-italy
#12
Silvia Ravagnan, Erika Carli, Eleonora Piseddu, Graziana Da Rold, Elena Porcellato, Claudia Zanardello, Antonio Carminato, Marta Vascellari, Gioia Capelli
BACKGROUND: Hemotropic mycoplasmas (hemoplasmas), the agents of infectious anemia, have been reported in dogs and cats. Little data are available on hemoplasma infections in Italy. The aim of this study was to evaluate the species of hemoplasmas and their prevalence in dogs and cats of northern Italy. METHODS: Blood samples were obtained from 117 candidate blood donor dogs, 278 free-roaming dogs and 227 free-roaming cats in 2014 and 2015. Samples were first screened for hemoplasmas with a SYBR green real time PCR...
March 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28202016/risk-factors-of-different-hemoplasma-species-infections-in-cats
#13
Michèle Bergmann, Theresa Englert, Bianca Stuetzer, Jennifer R Hawley, Michael R Lappin, Katrin Hartmann
BACKGROUND: Hemoplasma species (spp.) commonly cause infections in cats worldwide. However, data on risk factors for infections are limited. The aim of this study was to determine the prevalence of hemoplasma spp. infections in cats in Southern Germany and to assess risk factors associated with infection. RESULTS: DNA was extracted from blood samples of 479 cats presented to different veterinary hospitals for various reasons. DNA of feline hemoplasmas was amplified by use of a previously reported PCR assay...
February 16, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28158913/performance-of-4-point-of-care-screening-tests-for-feline-leukemia-virus-and-feline-immunodeficiency-virus
#14
J K Levy, P Cynda Crawford, S J Tucker
BACKGROUND: More than 3 million cats in the United States are infected with FeLV or FIV. The cornerstone of control is identification and segregation of infected cats. HYPOTHESIS/OBJECTIVES: To compare test performance with well-characterized clinical samples of currently available FeLV antigen/FIV antibody combination test kits. ANIMALS: Surplus serum and plasma from diagnostic samples submitted by animal shelters, diagnostic laboratories, veterinary clinics, and cat research colonies...
March 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28155290/the-diagnosis-of-bilateral-primary-renal-paragangliomas-in-a-cat
#15
Ryan B Friedlein, Alain J Carter, Robert D Last, Sarah Clift
A 9-year-old sterilised female domestic short-hair cat was referred with a history of vomiting and anorexia of 3 months' duration. Biochemistry, full-blood counts, thoracic radiographs, feline pancreatic-specific lipase, abdominal ultrasonography and feline immunodeficiency virus/feline leukaemia virus (FIV/FeLV) SNAP tests had been performed. Mild hypochloraemia and moderate hypokalaemia were evident on initial presentation. Abdominal ultrasonography initially revealed unilateral renal nodules on the left side...
January 24, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28131385/comparison-of-three-feline-leukaemia-virus-felv-point-of-care-antigen-test-kits-using-blood-and-saliva
#16
COMPARATIVE STUDY
Mark E Westman, Richard Malik, Evelyn Hall, Paul A Sheehy, Jacqueline M Norris
Feline leukaemia virus (FeLV) can be a challenging infection to diagnose due to a complex feline host-pathogen relationship and occasionally unreliable test results. This study compared the accuracy of three point-of-care (PoC) FeLV p27 antigen test kits commonly used in Australia and available commercially worldwide (SNAP FIV/FeLV Combo, Witness FeLV/FIV and Anigen Rapid FIV/FeLV), using detection of FeLV provirus by an in-house real-time polymerase chain reaction (qPCR) assay as the diagnostic gold standard...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28130406/prevalence-of-bartonella-species-infections-in-cats-in-southern-germany
#17
M Bergmann, T Englert, B Stuetzer, J R Hawley, M R Lappin, K Hartmann
Bartonella species are zoonotic pathogens, and infections in cats are common. However, prevalence in cats in Southern Germany is still unknown. Therefore, prevalence of Bartonella species DNA in blood of 479 Southern German cats was determined using a previously published conventional PCR targeting a fragment of the 16S-23S rRNA intergenic spacer region. Associations between Bartonella bacteraemia, housing conditions, feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) status, including progressive, regressive and abortive FeLV infection, were evaluated using Fisher's exact test...
April 1, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28107367/first-molecular-characterization-of-feline-immunodeficiency-virus-in-domestic-cats-from-mainland-china
#18
Jilei Zhang, Liang Wang, Jing Li, Patrick Kelly, Stuart Price, Chengming Wang
The feline immunodeficiency virus (FIV) is a retrovirus of the Lentivirus genus that was initially isolated from a colony of domestic cats in California in 1986 and has now been recognized as a common feline pathogen worldwide. To date, there is only one recent serology-based report on FIV in mainland China which was published in 2016. We designed this study to investigate the molecular prevalence and diversity of feline immunodeficiency virus (FIV) in domestic cats from mainland China. We studied the prevalence of FIV in whole blood samples of 615 domestic cats in five cities (Beijing, Guangzhou, Nanjing, Shanghai and Yangzhou) of mainland China and examined them using FRET-PCR (Fluorescence Resonance Energy Transfer-Polymerase Chain Reaction) and regular PCRs for the gag and env genes...
2017: PloS One
https://www.readbyqxmd.com/read/28039030/sexually-transmitted-infections-and-mate-finding-allee-effects
#19
Luděk Berec, Eva Janoušková, Michal Theuer
Infectious diseases can seriously impact dynamics of their host species. In this study, we model and analyze an interaction between a sexually transmitted infection and its animal host population affected by a mate-finding Allee effect. Since mating drives both host reproduction and infection transmission, the Allee effect shapes the transmission rate of the infection which we show takes a saturating form. Our model combining sexually transmitted infections with the mate-finding Allee effect in the host produces quite rich dynamics, including oscillations, several multistability regimes, and infection-induced host extinction...
April 2017: Theoretical Population Biology
https://www.readbyqxmd.com/read/27875092/morphological-variability-of-demodex-cati-in-a-feline-immunodeficiency-virus-positive-cat
#20
Elien R L Taffin, Stijn Casaert, Edwin Claerebout, Thomas J J Vandekerkhof, Sophie Vandenabeele
CASE DESCRIPTION A 17-year-old FIV-positive cat was evaluated because of weight loss during the preceding few months. The cat had a weight loss of 0.5 kg (1.1 lb) during the last month. Because of its FIV-positive status, the cat was confined indoors. CLINICAL FINDINGS A large nonpruritic area of alopecia with hyperpigmentation and comedones was present on the right lateral aspect of the neck. The chin had diffuse alopecia and comedones. Mild alopecia was present on the dorsal aspect of the muzzle. Trichography and microscopic examination of acetate tape imprint preparations and skin scrapings revealed a very morphologically heterogeneous population of Demodex mites...
December 1, 2016: Journal of the American Veterinary Medical Association
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