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https://www.readbyqxmd.com/read/27906587/differentiation-of-mouse-ovarian-stem-cells-toward-oocyte-like-structure-by-coculture-with-granulosa-cells
#1
Soraya Parvari, Hossein Yazdekhasti, Zahra Rajabi, Valliollah Gerayeli Malek, Tayebeh Rastegar, Mehdi Abbasi
An increasing body of evidence has confirmed existence and function of ovarian stem cells (OSCs). In this study, a novel approach on differentiation of OSCs into oocyte-like cells (OLCs) has been addressed. Recently, different methods have been recruited to isolate and describe aspects of OSCs, but newer and more convenient strategies in isolation are still growing. Herein, a morphology-based method was used to isolate OSCs. Cell suspension of mouse neonatal ovaries was cultured and formed colonies were harvested mechanically and cultivated on mouse embryonic fibroblasts...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27895165/rats-with-a-missense-mutation-in-atm-display-neuroinflammation-and-neurodegeneration-subsequent-to-accumulation-of-cytosolic-dna-following-unrepaired-dna-damage
#2
Hazel Quek, John Luff, KaGeen Cheung, Sergei Kozlov, Magtouf Gatei, C Soon Lee, Mark C Bellingham, Peter G Noakes, Yi Chieh Lim, Nigel L Barnett, Steven Dingwall, Ernst Wolvetang, Tomoji Mashimo, Tara L Roberts, Martin F Lavin
Mutations in the ataxia-telangiectasia (A-T)-mutated (ATM) gene give rise to the human genetic disorder A-T, characterized by immunodeficiency, cancer predisposition, and neurodegeneration. Whereas a series of animal models recapitulate much of the A-T phenotype, they fail to present with ataxia or neurodegeneration. We describe here the generation of an Atm missense mutant [amino acid change of leucine (L) to proline (P) at position 2262 (L2262P)] rat by intracytoplasmic injection (ICSI) of mutant sperm into oocytes...
November 28, 2016: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/27875264/expression-of-wnt4-5-during-reproductive-cycle-of-catfish-and-wnt5-promoter-analysis
#3
Yarikipati Prathibha, Balasubramanian Senthilkumaran
Signaling molecules, Wnt4 and Wnt5, are essential for ovarian growth during developmental stages in mammals. Although these molecules were identified in several teleosts, their precise expression and role in reproductive processes have not yet been explored in any lower vertebrates. In view of this, using catfish, Clarias batrachus as an animal model, cloning and expression analysis of wnt4 and wnt5 were analyzed in different tissues, at various developmental stages, during ovarian reproductive cycle and after gonadotropin induction...
January 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/27813674/induction-of-tissue-factor-pathway-inhibitor-2-by-hcg-regulates-periovulatory-gene-expression-and-plasmin-activity
#4
Muraly Puttabyatappa, Linah F Al-Alem, Farnosh Zakerkish, Katherine L Rosewell, Mats Brännström, Thomas E Curry
A key event during the process of ovulation is an increased proteolytic activity that aids in breakdown of the follicular wall to permit oocyte release. How the activity of these proteases is regulated is still not known. We hypothesize that Tissue Factor Pathway Inhibitor 2 (TFPI2), a matrix-associated Kunitz-type serine protease inhibitor, plays a role in regulating proteolytic activity during the periovulatory period as in other tissues. TFPI2 is secreted into the extracellular matrix (ECM) where it is postulated to regulate physiological ECM remodeling...
November 4, 2016: Endocrinology
https://www.readbyqxmd.com/read/27796004/ultrastructural-studies-on-oocyte-development-and-vitellogenesis-associated-with-follicle-cells-in-female-scapharca-subcrenata-pelecypoda-arcidae-in-western-korea
#5
Sung Han Kim
Ultrastructural studies on oocyte development and vitellogenesis in oocytes, and the functions of follicle cells during oogenesis and oocyte degeneration were investigated to clarifyb the reproductive mechanism on vitellogenesis of Scapharca subcrenata using electron microscope observations. In this study, vitellogenesis during oogenesis in the oocytes occured by way of autosynthesis and heterosynthesis. Of two processes of vitellogenesis during oogenesis, the process of endogenous autosynthesis involved the combined activity of the Golgi complex, mitochondria and rough endoplasmic reticulum...
September 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/27773884/efficient-mutagenesis-by-crispr-cas-system-during-meiotic-maturation-of-porcine-oocytes
#6
Asuka Onuma, Wataru Fujii, Koji Sugiura, Kunihiko Naito
Genome editing using the CRISPR/Cas system can induce mutations with high efficiency, and allows easier production of genome-modified animals than that offered by the conventional method where embryonic stem cells are used. However, studies using CRISPR/Cas systems have been mostly limited to proliferating somatic cells and pronuclear-stage fertilized eggs. In contrast, the efficiency of a CRISPR/Cas system in immature and maturing oocytes progressing through meiosis has not yet been assessed. In the present study, we evaluated the genome-modification efficiency of the CRISPR/Cas system during meiotic maturation of porcine oocytes...
October 21, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27767095/inhibitory-phosphorylation-of-cdk1-mediates-prolonged-prophase-i-arrest-in-female-germ-cells-and-is-essential-for-female-reproductive-lifespan
#7
Deepak Adhikari, Kiran Busayavalasa, Jingjing Zhang, Mengwen Hu, Sanjiv Risal, Mustafa Bilal Bayazit, Meenakshi Singh, M Kasim Diril, Philipp Kaldis, Kui Liu
A unique feature of female germ cell development in mammals is their remarkably long arrest at the prophase of meiosis I, which lasts up to 50 years in humans. Both dormant and growing oocytes are arrested at prophase I and completely lack the ability to resume meiosis. Here, we show that the prolonged meiotic arrest of female germ cells is largely achieved via the inhibitory phosphorylation of Cdk1 (cyclin-dependent kinase 1). In two mouse models where we have introduced mutant Cdk1(T14AY15F) which cannot be inhibited by phosphorylation (Cdk1AF) in small meiotically incompetent oocytes, the prophase I arrest is interrupted, leading to a premature loss of female germ cells...
October 21, 2016: Cell Research
https://www.readbyqxmd.com/read/27759816/randomized-double-blind-clinical-trial-comparing-two-anesthetic-techniques-for-ultrasound-guided-transvaginal-follicular-puncture
#8
Gilvandro Lins de Oliveira, Fernando Cesar Serralheiro, Fernando Luiz Affonso Fonseca, Onésimo Duarte Ribeiro, Fernando Adami, Denise Maria Christofolini, Bianca Bianco, Caio Parente Barbosa
Objective: To compare the anesthetic techniques using propofol and fentanyl versus midazolam and remifentanil associated with a paracervical block with lidocaine in performing ultrasound-guided transvaginal oocyte aspiration. Methods: A randomized double-blind clinical trial (#RBR-8kqqxh) performed in 61 women submitted to assisted reproductive treatment. The patients were divided into two groups: anesthetic induction with 1mcg/kg of fentanyl associated with 1.5mg/kg of propofol (FP Group, n=32), in comparison with anesthetic induction using 0...
July 2016: Einstein
https://www.readbyqxmd.com/read/27756766/involvement-of-pax2-in-ovarian-development-and-recrudescence-of-catfish-a-role-in-steroidogenesis
#9
Yarikipati Prathibha, Balasubramanian Senthilkumaran
PAX2, a member of paired box family, is an essential transcription factor for the organ development in vertebrates including teleosts, yet no evidence has been shown for its involvement in reproduction. To study this, partial- and/or full-length cDNA of pax2 was isolated from the ovary of catfish, Clarias batrachus, along with its other Pax family members, pax1 and pax9 Tissue distribution and ontogeny expression analysis indicated the prevalence of pax2 but not pax1 and pax9 in ovary. Varied phase-wise expression during ovarian cycle and elevation of pax2 after human chorionic gonadotropin induction showed probable regulation by gonadotropins...
December 2016: Journal of Endocrinology
https://www.readbyqxmd.com/read/27746868/the-effects-of-unilateral-varicose-ovarian-vein-on-antioxidant-capacity-and-oocyte-quality-in-rat-ovary
#10
Babatunde Adebayo Kehinde, Farid Abolhassani, Hossein Yazdekhasti, Niloufar Abbasi, Leyla Heydari, Erfan Daneshi, Zahra Rajabi, Alaa Hamada, Ashok Agarwal, Mehdi Abbasi
OBJECTIVES: Several researchers have reported the relationship between infertility in male and varicocele for so many years but the implication of varicocele in female patients is remains elusive. Here, we aim to examine the effects of unilateral varicose ovarian vein on antioxidant capacity and oocyte quality of rat ovary after the experimental creation of varicocele in female rats. MATERIALS AND METHODS: In this study, thirty adult female albino rats were divided into three equal groups: Group 1 as the control group has 10 rats, Group 2 as the sham group has 10 rats and they underwent a sham operation and finally Group 3 has the varicocele group has 10 rats...
August 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27738657/gnrh-agonist-trigger-versus-hcg-trigger-in-gnrh-antagonist-in-ivf-icsi-cycles-a-review-article
#11
Ashraf Alyasin, Shayesteh Mehdinejadiani, Marzieh Ghasemi
Routinely, a bolus of 5.000-10.000 IU human chorionic gonadotropin (hCG) is used for the final follicular maturation and ovulation as a standard method. HCG has the same effect of luteinizing hormone (LH) with long half-life. It has the long lutheotrophic effect which increases the risk of ovarian hyper stimulation syndrome (OHSS). Recently, gonadotropin-releasing hormone agonist (GnRH-a) trigger has been used for the induction of final follicular maturation and ovulation with the aim of reducing the OHSS risk...
September 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27728887/vitellogenin-induction-and-reduced-fecundity-in-zebrafish-exposed-to-effluents-from-the-city-of-bulawayo-zimbabwe
#12
Charles Teta, Yogeshkumar S Naik
Industrial and municipal effluents regularly pollute water bodies and cause various toxic effects to aquatic life. Because of the diverse nature of industrial processes and domestic products, urban effluents are often tainted with various anthropogenic endocrine disrupting chemicals that may interfere with the reproductive physiology of aquatic fauna. In this study, we tested effluents from the City of Bulawayo for the presence of estrogenic endocrine disrupting chemicals and their effects on fish gonads and fecundity...
October 8, 2016: Chemosphere
https://www.readbyqxmd.com/read/27720753/ovaprim-a-commercial-spawning-inducer-stimulates-gonadotropin-subunit-gene-transcriptional-activity-a-study-correlated-with-plasma-steroid-profile-ovulation-and-fertilization-in-the-catfish-heteropneustes-fossilis
#13
A Acharjee, R Chaube, K P Joy
The commercial fish spawning inducer Ovaprim (OVP) containing a salmon gonadotropin-releasing hormone analogue and domperidone (a dopamine receptor-2 antagonist) has been widely used as an effective spawning inducer in artificial breeding of fishes. It induces a preovulatory LH surge resulting in final oocyte maturation (FOM) and ovulation through a mechanism involving a steroidogenic shift to secrete a maturation-inducing steroid (MIS). In the present study, a 0.5μL/g body weight dose of OVP each injected at 0h and 24h intraperitoneally into gravid female catfish, Heteropneustes fossilis resulted in periovulatory changes in gonadotropin (GtH) subunit gene expression and steroid hormone levels...
October 5, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27720752/in-vivo-induction-of-human-chorionic-gonadotropin-by-osmotic-pump-advances-sexual-maturation-during-pre-spawning-phase-in-adult-catfish
#14
Raju Murugananthkumar, Yarikipati Prathibha, Balasubramanian Senthilkumaran, Anbazhagan Rajakumar, Hirohiko Kagawa
Gonadal maturation is a critical event wherein gonads, under the influence of several hormones and factors, undergo cyclic morphological and physiological changes to produce functional gametes during the spawning phase. However, artificial induction can be effectively used to advance the maturation of gonad vis-à-vis spawning like behavior in seasonal breeders during the off-breeding season. In the present study, osmotic pumps loaded with 5000IU of human chorionic gonadotropin (hCG) or saline as control were implanted intraperitoneally for 21days during the pre-spawning phase (May-June) in catfish Clarias batrachus and C...
October 5, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27717202/induction-of-multiple-ovulation-via-modulation-of-angiotensin-ii-receptors-in-in-vitro-ovarian-follicle-culture-models
#15
Yong Jin Kim, Yoon Young Kim, Byeong-Cheol Kang, Moon Suk Kim, In Kap Ko, Hung Ching Liu, Zev Rosenwaks, Seung-Yup Ku
In vitro culture of ovarian follicles is a promising bioengineering technique for retrieving fertilizable oocytes from preserved ovarian tissues of cancer survivors. However, current in vitro follicle culture techniques are labour-intensive and of low efficiency, as only single follicle culture (SFC) has been possible to date. The present study investigated the feasibility of multifollicular cluster culture (MFCC) system using angiotensin II receptor (ATII-Rc) analogues. Ovarian pre-antral follicles isolated from 2-week-old C57BL6 mice were cultured with ATII-Rc agonist or antagonist and their maturation outcomes were compared with control group...
September 15, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27692521/-does-hyperandrogenic-statute-in-the-polycystic-ovary-syndrome-constitute-an-obstacle-to-the-success-of-in-vitro-fertilization
#16
M Gaddas, N Chaouache, M Ajina, Z Tabka, M Zaouali Ajina
OBJECTIVES: The aim of this study was to evaluate the impact of initial androgenic statute of women with polycystic ovary syndrome (SOPK) on the expected results of in vitro fertilization (FIV). MATERIALS AND METHODS: It is about an exploratory study carried out in 61 unfertile SOPK patients, having profited all from a cycle of FIV/ICSI (intracytoplasmic sperm injection). We have divided our population in two groups according to their hormonal androgenic statute: groups with hyperandrogenism and without hyperandrogenism...
September 28, 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
https://www.readbyqxmd.com/read/27677591/retinoic-acid-and-meiosis-induction-in-adult-versus-embryonic-gonads-of-medaka
#17
Mateus C Adolfi, Amaury Herpin, Martina Regensburger, Jacopo Sacquegno, Joshua S Waxman, Manfred Schartl
In vertebrates, one of the first recognizable sex differences in embryos is the onset of meiosis, known to be regulated by retinoic acid (RA) in mammals. We investigated in medaka a possible meiotic function of RA during the embryonic sex determination (SD) period and in mature gonads. We found RA mediated transcriptional activation in germ cells of both sexes much earlier than the SD stage, however, no such activity during the critical stages of SD. In adults, expression of the RA metabolizing enzymes indicates sexually dimorphic RA levels...
September 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27650588/induction-of-gonadal-maturation-at-different-temperatures-in-burbot-lota-lota
#18
F J Schaefer, B Hermelink, P Husmann, W Meeus, J Adriaen, S Wuertz
A rearing experiment was conducted to test whether temperature protocols that differed from a simulation of natural conditions might induce maturation after isothermal grow-out in burbot Lota lota. Lota lota were acclimated to two different temperature regimes: low temperature (LT), close to natural temperature at 4·0° C and elevated, high temperature (HT) at 8·5° C over 40 and 27 days respectively, with all fish then wintered for 47 days. Every second fish was treated with a gonadotropin-releasing hormone analogue...
September 20, 2016: Journal of Fish Biology
https://www.readbyqxmd.com/read/27629151/conversion-of-goat-fibroblasts-into-lineage-specific-cells-using-a-direct-reprogramming-strategy
#19
Yanjie Guo, Tong Yu, Lei Lei, Anqin Duan, Xiaoling Ma, Huayan Wang
Direct reprogramming is an efficient strategy to convert one cell type to another. In this study, due to the failure of maintaining the undifferentiated state of goat embryotic stem- and induced pluripotent stem-like cells in vitro, we explored an alternative way to directly convert goat fibroblasts to lineage-specific cells. The 'Yamanaka factors' was ectopically expressed in fibroblasts for a short term to situate cells in a metastable state. By culturing with lineage-specific media for 1-2 weeks, the cardiomyocyte-like cells and neurocyte-like cells were generated and confirmed by the quantitative RT-PCR and immunocytochemical staining...
September 15, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/27614971/roles-of-organic-anion-transporters-oats-in-renal-proximal-tubules-and-their-localization
#20
Naoyuki Otani, Motoshi Ouchi, Keitaro Hayashi, Promsuk Jutabha, Naohiko Anzai
Organic anions (OAs) are secreted in renal proximal tubules in two steps. In the first step, OAs are transported from the blood through basolateral membranes into proximal tubular cells. The prototypical substrate for renal organic anion transport systems, para-aminohippurate (PAH), is transported across basolateral membranes of proximal tubular cells via OAT1 (SLC22A6) and OAT3 (SLC22A8) against an electrochemical gradient in exchange for intracellular dicarboxylates. In the second step, OAs exit into urine through apical membranes of proximal tubules...
September 10, 2016: Anatomical Science International
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