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Oocytes induction

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https://www.readbyqxmd.com/read/29151336/efficacy-of-bispectral-index-monitoring-for-prevention-of-anestheticawareness-and-complications-during-oocyte-pick-up-procedure
#1
Aykut Urfalıoğlu, Mahmut Arslan, Murat Bakacak, Hafize Öksüz, Ömer Faruk Boran, Gözen Öksüz
Background/aim: This study was planned by considering that the use of bispectral index (BIS) monitoring ensures sufficient depth of anesthesia and avoids anesthetic awareness and patient movement in the oocyte pick-up (OPU) procedure.Materials and methods: Ninety-eight patients undergoing OPU were randomly divided into 2 groups as the control group (n = 48) and BIS group (n = 50). After propofol and remifentanil induction, the control group was given additional propofol according to reaction response, while the BIS group was given propofol at BIS values of 60 and above with the aim that BIS values be 40?60...
November 13, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29149414/role-of-tctp-for-cellular-differentiation-and-cancer-therapy
#2
Ean-Jeong Seo, Nicolas Fischer, Thomas Efferth
The translationally controlled tumor protein (TCTP) is a highly conserved protein that is regulated due to a high number of extracellular stimuli. TCTP has an important role for cell cycle and normal development. On the other side, tumor reversion and malignant transformation have been associated with TCTP. TCTP has been found among the 12 genes that are differentially expressed during mouse oocyte maturation, and an overexpression of this gene was reported in a wide variety of different cancer types. Its antiapoptotic effect is indicated by the interaction with several proapoptotic proteins of the Bcl-2 family and the p53 tumor suppressor protein...
2017: Results and Problems in Cell Differentiation
https://www.readbyqxmd.com/read/29147493/the-effect-of-the-ovarian-varicose-vein-on-the-dna-methylation-in-the-rat-s-oocyte
#3
Amirhossein Mohammadi, Bagher Minaei Zangi, Mahshid Delfan Azari, Rafieh Alizadeh, Mohammad Salehi, Erfan Daneshi, Mohammad Jafar Rezaei, Mehdi Abbasi
Objectives: We intended to determine whether the ovarian varicose which is one of the common etiologies of the pelvic congestion syndrome, has the ability to interfere with the DNA methylation reprogramming in the oocyte and thereby affect the oocyte quality or not. Materials and Methods: Varicose model was induced according to the Turner's method in the rats. Briefly, a 20-gauge needle was placed on the left renal vein and a thread was tied over both the needle and the renal vein medial to the insertion of the ovarian vein, and then the needle was removed...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29132734/role-of-vitamin-e-and-d3-supplementation-in-intra-cytoplasmic-sperm-injection-outcomes-of-women-with-polycystic-ovarian-syndrome-a-double-blinded-randomized-placebo-controlled-trial
#4
Farnaz Fatemi, Afsaneh Mohammadzadeh, Mohammad Reza Sadeghi, Mohammad Mehdi Akhondi, Shayan Mohammadmoradi, Koorosh Kamali, Nicknam Lackpour, Sheida Jouhari, Simin Zafadoust, Sara Mokhtar, Ladan Giahi
BACKGROUND & AIMS: Ovulation induction has been proven to impose oxidative stress during ICSI treatment cycle. Also, data indicates that PCOS women show higher oxidative markers. Available data are not convincing about which antioxidant supplements have the potential to overcome oxidative stress in PCOS subjects. The aim of this trial was to investigate the possible role of combined vitamin E and D supplementation in the ICSI outcomes (oocyte number and quality, embryo number and quality, pregnancy rate) of PCOS subjects...
April 2017: Clinical Nutrition ESPEN
https://www.readbyqxmd.com/read/29132339/sequential-e2-levels-not-ovarian-maximal-diameter-estimates-were-correlated-with-outcome-of-cetrotide-therapy-for-management-of-women-at-high-risk-of-ovarian-hyperstimulation-syndrome-a-randomized-controlled-study
#5
Khalid M Salama, Hesham M Abo Ragab, Mohammed F El Sherbiny, Ali A Morsi, Ibrahim I Souidan
BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is an important condition with considerable morbidity and a small risk of mortality and most commonly results as an iatrogenic condition following follicular stimulation of the ovaries. We aimed to evaluate safety and efficacy of 3-day cetrotide therapy started on day of oocyte retrieval (Day-0) in women at high-risk for development of ovarian hyperstimulation syndrome (OHSS) after GnRH agonist induction protocol. METHODS: Forty-eight women fulfilling inclusion criteria underwent ultrasound scanning for maximal ovarian diameter (MOD) estimation and ascites grading...
November 13, 2017: BMC Women's Health
https://www.readbyqxmd.com/read/29122660/a-potential-role-of-galectin-1-in-promoting-mouse-trophoblast-stem-cell-differentiation
#6
Jia-Li You, Wei Wang, Min-Yue Tang, Ying-Hui Ye, Ai-Xia Liu, Yi-Min Zhu
Galectin-1 is highly expressed in blastocysts and trophoblast giant cells during implantation, and dysregulated galectin-1 is associated with many pregnancy-related abnormalities. Elevated galectin-1 contributes to cancer cells invasion. Here, we found that galectin-1 is expressed in mouse oocytes, preimplantation embryos (all stages), and trophoblast stem (TS) cells. Peak levels of galectin-1 mRNA and protein were detected on day 4 and day 5 after the induction of TS cells differentiation. Overexpression of galectin-1 increased TS cells migration and invasion, whereas knockdown of galectin-1 attenuated these effects...
November 6, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29113725/hormonal-induction-of-ovulation-using-ovaprim%C3%A2-d-arg-6-pro-9-net-sgnrh-domperidone-and-its-impact-on-embryonic-development-of-wild-caught-longspine-scraper-capoeta-trutta-heckel-1843
#7
Vahid Zadmajid, Rahman Mirzaee, Hamid Hoseinpour, Nazir Vahedi, Ian Anthony Ernest Butts
Knowledge of gamete quality is a prerequisite for developing techniques to fertilize eggs and rear offspring for hatchery production. Our objective was to develop assisted reproductive techniques, via hormonal induction of final oocyte maturation (FOM), for Longspine scraper, Capoeta trutta. Fish were administered injections of salmon gonadotropin-releasing hormone analogue containing anti-dopaminergic drug (Ovaprim™) or saline (control). Effects of Ovaprim on induction of ovulation, gamete quality, embryonic development, and larval survival were later examined with serum steroid hormone levels and ovarian histology...
October 23, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29113581/comparison-of-follicle-isolation-methods-for-mouse-ovarian-follicle-culture-in-vitro
#8
Eun Jung Kim, Jaewang Lee, Hye Won Youm, Seul Ki Kim, Jung Ryeol Lee, Chang Suk Suh, Seok Hyun Kim
Ovarian follicle in vitro culture is a promising fertility preservation option to avoid risk of reintroduction of malignant cells. The objective of this study is to compare 4 different follicle isolation methods from ovarian tissue and evaluate the effect of follicle isolation on further in vitro follicle culture and oocyte competency. Mouse ovaries were dissected and randomly divided into 4 groups according to follicle isolation method: mechanical (MCH) isolation, mincing (MNC) isolation, enzymatically digestion using collagenase (COL), and enzymatically digestion using liberase (LIB)...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/29109171/postovulatory-aging-causes-the-deterioration-of-porcine-oocytes-via-induction-of-oxidative-stress
#9
Yilong Miao, Changyin Zhou, Zhaokang Cui, Mianqun Zhang, Xiayan ShiYang, Yajuan Lu, Bo Xiong
Negative effects of postovulatory aging on fertilization ability and subsequent embryo development have been reported in rodents; however, the molecular and cellular changes during this process have not been fully defined. Here, we used porcine oocytes, a model that is physiologically and developmentally similar to humans, to explore the molecular mechanisms that underlie how postovulatory aging affects oocyte quality and fertilization capacity. We found that postovulatory aging caused the morphologic change of porcine oocytes by exhibiting the incompact expansion of cumulus cells and an increased occurrence of fragmentation...
November 6, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29096046/oral-medications-including-clomiphene-citrate-or-aromatase-inhibitors-with-gonadotropins-for-controlled-ovarian-stimulation-in-women-undergoing-in-vitro-fertilisation
#10
REVIEW
Mohan S Kamath, Abha Maheshwari, Siladitya Bhattacharya, Kar Yee Lor, Ahmed Gibreel
BACKGROUND: Gonadotropins are the most commonly used medications for controlled ovarian stimulation in in vitro fertilisation (IVF). However, they are expensive and invasive, and are associated with the risk of ovarian hyperstimulation syndrome (OHSS). Recent calls for more patient-friendly regimens have led to growing interest in the use of clomiphene citrate (CC) and aromatase inhibitors with or without gonadotropins to reduce the burden of hormonal injections. It is currently unknown whether regimens using CC or aromatase inhibitors such as letrozole (Ltz) are as effective as gonadotropins alone...
November 2, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29043702/n-acetylcysteine-compared-to-metformin-improves-the-expression-profile-of-growth-differentiation-factor-9-and-receptor-tyrosine-kinase-c-kit-in-the-oocytes-of-patients-with-polycystic-ovarian-syndrome
#11
Ebrahim Cheraghi, Malek Soleimani Mehranjani, Seyed Mohammad Ali Shariatzadeh, Mohammad Hossein Nasr Esfahani, Behrang Alani
Background: Paracrine disruption of growth factors in women with polycystic ovarian syndrome (PCOS) results in production of low quality oocyte, especially following ovulation induction. The aim of this study was to investigate the effects of metformin (MET), N-acetylcysteine (NAC) and their combination on the hormonal levels and expression profile of GDF-9, BMP-15 and c-kit, as hallmarks of oocyte quality, in PCOS patients. MATERIALS AND METHODS: This prospective randomized, double-blind, placebo controlled trial aims to study the effects of MET, NAC and their combination (MET+NAC) on expression of GDF-9, BMP-15 and c-kit mRNA in oocytes [10 at the germinal vesicle (GV) stage, 10 at the MI stage, and 10 at the MII stage from per group] derived following ovulation induction in PCOS...
January 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29029246/the-epidermal-growth-factor-network-role-in-oocyte-growth-maturation-and-developmental-competence
#12
Dulama Richani, Robert B Gilchrist
BACKGROUND: The LH surge induces great physiological changes within the preovulatory follicle, which culminate in the ovulation of a mature oocyte that is capable of supporting embryo and foetal development. However, unlike mural granulosa cells, the oocyte and its surrounding cumulus cells are not directly responsive to LH, indicating that the LH signal is mediated by secondary factors produced by the granulosa cells. The mechanisms by which the oocyte senses the ovulatory LH signal and hence prepares for ovulation has been a subject of considerable controversy for the past four decades...
September 20, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/29025092/heat-stress-induces-autophagy-in-pig-ovaries-during-follicular-development
#13
Benjamin J Hale, Candice L Hager, Jacob T Seibert, Joshua T Selsby, Lance H Baumgard, Aileen F Keating, Jason W Ross
Hyperthermia or heat stress (HS) occurs when heat dissipation mechanisms are overwhelmed by external and internal heat production. Hyperthermia negatively affects reproduction and potentially compromises oocyte integrity and reduces developmental competence of ensuing embryos. Autophagy is the process by which cells recycle energy through the reutilization of cellular components and is activated by a variety of stressors. Study objectives were to characterize autophagy-related proteins in the ovary following cyclical HS during the follicular phase...
September 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28960749/detoxification-of-ivermectin-by-atp-binding-cassette-transporter-c4-and-cytochrome-p450-monooxygenase-6cj1-in-the-human-body-louse-pediculus-humanus-humanus
#14
J H Kim, K J Gellatly, B Lueke, M Kohler, R Nauen, E Murenzi, K S Yoon, J M Clark
We previously observed that ivermectin-induced detoxification genes, including ATP binding cassette transporter C4 (PhABCC4) and cytochrome P450 6CJ1 (CYP6CJ1) were identified from body lice following a brief exposure to a sublethal dose of ivermectin using a non-invasive induction assay. In this current study, the functional properties of PhABCC4 and CYP6CJ1 were investigated after expression in either X. laevis oocytes or using a baculovirus expression system, respectively. Efflux of [(3) H]-9-(2-phosphonomethoxyethyl) adenine ([(3) H]-PMEA), a known ABCC4 substrate in humans, was detected from PhABCC4 cRNA-injected oocytes by liquid scintillation spectrophotometric analysis and PhABCC4 expression in oocytes was confirmed using ABC transporter inhibitors...
September 27, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28934084/outcome-of-icsi-with-motile-testicular-spermatozoa-obtained-through-microscopically-assisted-testicular-sperm-extraction-in-relation-to-the-ovarian-response
#15
E Erdem, M Karacan, A Usta, A Arvas, Z Cebi, T Camlibel
INTRODUCTION: To determine the relationship between AFC, basal FSH level, woman's age, the number of oocytes retrieved and the outcome of ICSI with testicular spermatozoa obtained with microscopically assisted testicular sperm extraction. MATERIAL AND METHODS: In this retrospective cohort study, a total of 340 couples who underwent ICSI treatment with testicular sperm were enrolled. Women aged?40years and the first cycles of couples were included. ICSI was performed with motile testicular spermatozoa obtained from 89 men with obstructive azoospermia and 251 men with nonobstructive azoospermia...
May 2017: Journal of gynecology obstetrics and human reproduction
https://www.readbyqxmd.com/read/28928204/bone-morphogenetic-protein-and-retinoic-acid-synergistically-specify-female-germ-cell-fate-in-mice
#16
Hidetaka Miyauchi, Hiroshi Ohta, So Nagaoka, Fumio Nakaki, Kotaro Sasaki, Katsuhiko Hayashi, Yukihiro Yabuta, Tomonori Nakamura, Takuya Yamamoto, Mitinori Saitou
The mechanism for sex determination in mammalian germ cells remains unclear. Here, we reconstitute the female sex determination in mouse germ cells in vitro under a defined condition without the use of gonadal somatic cells. We show that retinoic acid (RA) and its key effector, STRA8, are not sufficient to induce the female germ-cell fate. In contrast, bone morphogenetic protein (BMP) and RA synergistically induce primordial germ cells (PGCs)/PGC-like cells (PGCLCs) derived from embryonic stem cells (ESCs) into fetal primary oocytes...
November 2, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28915715/epigenetic-regulation-during-the-differentiation-of-stem-cells-to-germ-cells
#17
REVIEW
Yuan-Chao Sun, Yong-Yong Wang, Wei Ge, Shun-Feng Cheng, Paul W Dyce, Wei Shen
Gametogenesis is an essential process to ensure the transfer of genetic information from one generation to the next. It also provides a mechanism by which genetic evolution can take place. Although the genome of primordial germ cells (PGCs) is exactly the same with somatic cells within an organism, there are significant differences between their developments. For example, PGCs eventually undergo meiosis to become functional haploid gametes, and prior to that they undergo epigenetic imprinting which greatly alter their genetic regulation...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28893570/phytoestrogenic-effect-of-inula-racemosa-hook-f-a-cardioprotective-root-drug-in-traditional-medicine
#18
Mangathayaru Kalachaveedu, Divya Raghavan, Srivani Telapolu, Sarah Kuruvilla, Balakrishna Kedike
ETHNOPHARMACOLOGICAL RELEVANCE: Roots of Inula racemosa are used as a cardio protective in Ayurveda in India, being prescribed as a medicine for precordial chest pain, cough and dyspnoea, both singly and as a poly herbal. AIM: Evaluation of Phytoestrogenic activity of the root extracts of Inula racemosa and compounds isolated therefrom in vivo, in silico and in vitro. MATERIALS AND METHODS: Alcohol (IrA) and hexane (IrH) extracts characterized by HPTLC/GC-MS analysis respectively and processed for compound isolation were evaluated for estrogenic activity (100 & 250mg/kg bw) by the Immature rat uterotrophic assay using ethinylestradiol (EE -30µg/kg bw) as standard drug...
September 8, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28855574/genetic-studies-in-mice-directly-link-oocytes-produced-during-adulthood-to-ovarian-function-and-natural-fertility
#19
Ning Wang, Chonthicha Satirapod, Yasuyo Ohguchi, Eun-Sil Park, Dori C Woods, Jonathan L Tilly
Multiple labs have reported that mammalian ovaries contain oogonial stem cells (OSCs), which can differentiate into oocytes that fertilize to produce offspring. However, the physiological relevance of these observations to adult ovarian function is unknown. Here we performed targeted and reversible ablation of premeiotic germ cells undergoing differentiation into oocytes in transgenic mice expressing the suicide gene, herpes simplex virus thymidine kinase (HSVtk), driven by the promoter of stimulated by retinoic acid gene 8 (Stra8), a germ cell-specific gene activated during meiotic commitment...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28853096/induced-aneugenic-effects-in-mouse-oocytes-in-vivo
#20
K L Pligina, A K Zhanataev, A V Kulakova, N O Daugel-Dauge, A D Durnev
Aneugenic effects of the chemicals with antitumor activity were studied in mouse oocytes in vivo by cytogenetic analysis. In control mice, no oocytes with numerical chromosome aberrations were found. Colchicine (0.2-4 mg/kg), paclitaxel (2.5-7.5 mg/kg), and etoposide (10-60 mg/kg) produced a significant dose-dependent aneugenic effects (induction of up to 25% aneuploid oocytes) and increased the yield of oocytes arrested in the meiotic MI stage and with premature separation of sister chromatid. Paclitaxel induced up to 20% polyploid chromosomes...
August 29, 2017: Bulletin of Experimental Biology and Medicine
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