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Oocytes induction

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https://www.readbyqxmd.com/read/28193316/polydatin-improves-the-developmental-competence-of-bovine-embryos-in-vitro-via-induction-of-sirtuin-1-sirt1
#1
Imran Khan, Sung Woo Kim, Kyung-Lim Lee, Seok-Hwan Song, Ayman Mesalam, M M R Chowdhury, Zia Uddin, Ki Hun Park, Il-Keun Kong
The aim of the present study was to investigate the beneficial effect of polydatin (PD), the glycoside form of resveratrol, on embryo development in vitro. Oocytes were aspirated from ovaries of Korean Hanwoo cows and cultured until Day 8 in a humidified atmosphere of 5% CO2 in air at 38.5°C. Protein and gene expression levels were determined through confocal microscopy and reverse transcription-polymerase chain reaction respectively, whereas the number of total and apoptotic cells in Day 8 blastocysts was determined using Hoechst 33342 staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling...
February 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28185602/impaired-hypothalamic-pituitary-testicular-axis-activity-spermatogenesis-and-sperm-function-promote-infertility-in-males-with-lead-poisoning
#2
Jason Gandhi, Rafael J Hernandez, Andrew Chen, Noel L Smith, Yefim R Sheynkin, Gargi Joshi, Sardar Ali Khan
Lead poisoning is a stealthy threat to human physiological systems as chronic exposure can remain asymptomatic for long periods of time before symptoms manifest. We presently review the biophysical mechanisms of lead poisoning that contribute to male infertility. Environmental and occupational exposure of lead may adversely affect the hypothalamic-pituitary-testicular axis, impairing the induction of spermatogenesis. Dysfunction at the reproductive axis, namely testosterone suppression, is most susceptible and irreversible during pubertal development...
February 10, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28144637/induction-of-fas-mediated-apoptosis-by-interferon-%C3%AE-is-dependent-on-granulosa-cell-differentiation-and-follicular-maturation-in-the-rat-ovary
#3
Hye-Jeong Lee, Ji Young Kim, Ji Eun Park, Yong-Dal Yoon, Benjamin K Tsang, Jong-Min Kim
Fas ligand (FasL) and its receptor Fas have been implicated in granulosa cell apoptosis during follicular atresia. Although interferon-gamma (IFN-γ) is believed to be involved in the regulation Fas expression in differentiated granulosa or granulosa-luteal cells, the expression of this cytokine and its role in the regulation of the granulosa cell Fas/FasL system and apoptosis during follicular maturation have not been thoroughly investigated. In the present study, we have examined the presence of IFN-γ in ovarian follicles at different stage of development by immunohistochemistry and related their relative intensities with follicular expression of Fas and FasL, and with differences in granulosa cell sensitivity to Fas activation by exogenous agonistic Anti-Fas monoclonal antibody (Fas mAb)...
December 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28112985/comparison-of-human-amniotic-chorionic-and-umbilical-cord-multipotent-mesenchymal-stem-cells-regarding-their-capacity-for-differentiation-toward-female-germ-cells
#4
Hamid Reza Asgari, Mohammad Akbari, Hossein Yazdekhasti, Zahra Rajabi, Shadan Navid, Fereshte Aliakbari, Niloufar Abbasi, Freidon Sargolzaei Aval, Alireza Shams, Mehdi Abbasi
Placenta harbors a plentiful source of various cells with stem cells or stem-like cell properties, which can be used in therapeutic procedures and research. Mesenchymal stem cells (MSCs) have attracted much attention due to their specific differentiation potential and tolerogenic properties. MSCs have been isolated from different parts of placenta; however, in this study, we isolated MSCs from amnion and chorion membrane, as well as umbilical cord (Wharton's jelly [WJ]) and compared their capacity regarding differentiation toward female germ cells under influence of 10 ng/mL BMP4...
February 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28070808/combined-positive-effect-of-oocyte-extracts-and-brilliant-cresyl-blue-stained-recipient-cytoplasts-on-epigenetic-reprogramming-and-gene-expression-in-buffalo-nuclear-transfer-embryos
#5
E M Sadeesh, Shah Fozia, Kataria Meena
This study examined the effects of buffalo oocyte extracts (BOE) on donor cells reprogramming and molecular characterisation of oocytes screened via brilliant cresyl blue (BCB) staining and comparison of gene expression profiles of developmentally important genes in blastocysts from IVF and cloned derived from BOE treated donor cells with BCB selected recipient cytoplasts. Relative abundance (RA) of OCT4 and NANOG was increased (P < 0.05) and HDAC-1, DNMT-1, and DNMT-3A decreased (P < 0.05) in extract treated cells (ETCs)...
January 9, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28069903/improvement-of-in-vitro-produced-bovine-embryo-treated-with-coa-a-under-heat-stressed-condition
#6
Imran Khan, Kyeong-Lim Lee, Lianguang Xu, Ayman Mesalam, Mmr Chowdhury, Myeong-Don Joo, Ihsan-Ul- Haq, Bushra Mirza, Il-Keun Kong
Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during in vitro production of bovine embryos. Oocytes were incubated in in vitro maturation (IVM) media with or without coa-A at two different temperatures, 40.5 °C and 42 °C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5 °C (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-kB) and cyclooxygenase-2 (COX2)...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28050961/final-oocyte-maturation-in-assisted-reproduction-with-human-chorionic-gonadotropin-and-gonadotropin-releasing-hormone-agonist-dual-trigger
#7
REVIEW
Sofia Andrade de Oliveira, Vinícius Fernando Calsavara, Gemma Castillón Cortés
Final oocyte maturation with Human Chorionic Gonadotropin (hCG) and ovarian stimulation with Follicle Stimulation Hormone (FSH) combined with Gonadotrophin-releasing Hormone (GnRH) antagonist to block Luteinizing hormone (LH) surge is a standard procedure of in vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI). However, GnRH agonist has been replacing the use of hCG in certain situations, especially in patients at risk of Ovarian Hyperstimulation Syndrome (OHSS). Some studies have also shown advantages in the combined use of GnRH agonist concurrently with hCG in inducing final oocyte maturation, a treatment known as "Dual Trigger"...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28043371/sperm-bound-antisperm-antibodies-prevent-capacitation-of-bovine-spermatozoa
#8
M S Ferrer, J Klabnik-Bradford, D E Anderson, A C Bullington, R A Palomares, L M J Miller, R Stawicki, M Miesner
It was hypothesized here that sperm-bound antisperm antibodies (ASAs) impair the ability of bovine spermatozoa to undergo capacitation, bind to the zona pellucida, and complete the acrosome reaction. The effect of ASA binding on these functions was evaluated in frozen/thawed spermatozoa from four bulls before and after induction of ASAs. Ejaculates were divided into ASA negative (<10% immunoglobulin [Ig]G- and IgA-bound spermatozoa) or ASA positive (≥20% IgG and/or IgA-bound spermatozoa). The percentage of capacitated (Merocyanine 540 positive) live spermatozoa in response to heparin was lower in ASA-positive than ASA-negative ejaculates (P < 0...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/27998857/-clinical-outcome-of-in-vitro-fertilization-or-intracytoplasmic-sperm-injection-embryo-transfer-in-women-aged-40-years-and-above
#9
Xing-Yu Zhou, Shu-Xian Feng, Xue-Lan Li, Yu-Dong Liu, Xin Chen, De-Sheng Ye, Jing Niu, Shi-Ling Chen
OBJECTIVE: To investigate the clinical outcomes in vitro fertilization or intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) in women aged over 40 years. METHODS: We retrospectively analyzed 1050 non-donor IVF/ICSI-ET cycles performed from January, 2007 to December, 2015 in women at the age 40 years or above, including 393 women at 40 years of age, 266 at 41 years, 158 at 42 years, 107 at 43 years, 64 at 44 years, and 65 at 45-51 years. The clinical characteristics and outcomes of the women in different age groups were compared and analyzed...
December 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27988256/oocyte-competence-in-women-with-polycystic-ovary-syndrome
#10
REVIEW
Stefano Palomba, Jessica Daolio, Giovanni Battista La Sala
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age and affects fertility and pregnancy in cases of oligoanovulation. Ovulation induction is often used to treat anovulatory patients with PCOS, but many of these women fail to conceive and resort to assisted reproductive technologies. Alterations in oocyte competence (OC) are considered potential causative factors for subfertility in women with PCOS. In this review we present and critically assess all recent clinical and experimental data regarding OC in women with PCOS...
December 14, 2016: Trends in Endocrinology and Metabolism: TEM
https://www.readbyqxmd.com/read/27974271/silver-nanoparticles-induce-oocyte-maturation-in-zebrafish-danio-rerio
#11
Shi Xi Chen, Xiao Zhen Yang, Ying Deng, Jing Huang, Yan Li, Qian Sun, Chang-Ping Yu, Yong Zhu, Wan Shu Hong
Public concern regarding silver nanoparticles (AgNPs) in the environment has been increasing since they can cause adverse effects in some aquatic species. However, few data are actually available on the effects of AgNPs on the germ cells. In the present study, we used the zebrafish ovarian follicle as a model to assess the potentially adverse effects of AgNPs on oocyte maturation (germinal vesicle breakdown, GVBD) in vitro. Similar to the maturation inducing hormone (17α, 20β-dihydroxy-4-pregnen-3-one), AgNPs induced GVBD, and reduced the total cyclic adenosine monophosphate (cAMP) concentration in zebrafish ovarian follicles...
March 2017: Chemosphere
https://www.readbyqxmd.com/read/27943108/associations-between-ivf-outcomes-and-essential-trace-elements-measured-in-follicular-fluid-and-urine-a-pilot-study
#12
Mary E Ingle, Michael S Bloom, Patrick J Parsons, Amy J Steuerwald, Pamela Kruger, Victor Y Fujimoto
PURPOSE: A hypothesis-generating pilot study exploring associations between essential trace elements measured in follicular fluid (FF) and urine and in vitro fertilization (IVF) endpoints. METHODS: We recruited 58 women undergoing IVF between 2007 and 2008, and measured cobalt, chromium, copper, manganese, molybdenum, and zinc in FF (n = 46) and urine (n = 45) by inductively coupled plasma mass spectrometry (ICP-MS). We used multivariable regression models to assess the impact of FF and urine trace elements on IVF outcomes, adjusted for age, body mass index, race, and cigarette smoking...
February 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27906587/differentiation-of-mouse-ovarian-stem-cells-toward-oocyte-like-structure-by-coculture-with-granulosa-cells
#13
Soraya Parvari, Hossein Yazdekhasti, Zahra Rajabi, Valliollah Gerayeli Malek, Tayebeh Rastegar, Mehdi Abbasi
An increasing body of evidence has confirmed existence and function of ovarian stem cells (OSCs). In this study, a novel approach on differentiation of OSCs into oocyte-like cells (OLCs) has been addressed. Recently, different methods have been recruited to isolate and describe aspects of OSCs, but newer and more convenient strategies in isolation are still growing. Herein, a morphology-based method was used to isolate OSCs. Cell suspension of mouse neonatal ovaries was cultured and formed colonies were harvested mechanically and cultivated on mouse embryonic fibroblasts...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27895165/rats-with-a-missense-mutation-in-atm-display-neuroinflammation-and-neurodegeneration-subsequent-to-accumulation-of-cytosolic-dna-following-unrepaired-dna-damage
#14
Hazel Quek, John Luff, KaGeen Cheung, Sergei Kozlov, Magtouf Gatei, C Soon Lee, Mark C Bellingham, Peter G Noakes, Yi Chieh Lim, Nigel L Barnett, Steven Dingwall, Ernst Wolvetang, Tomoji Mashimo, Tara L Roberts, Martin F Lavin
Mutations in the ataxia-telangiectasia (A-T)-mutated (ATM) gene give rise to the human genetic disorder A-T, characterized by immunodeficiency, cancer predisposition, and neurodegeneration. Whereas a series of animal models recapitulate much of the A-T phenotype, they fail to present with ataxia or neurodegeneration. We describe here the generation of an Atm missense mutant [amino acid change of leucine (L) to proline (P) at position 2262 (L2262P)] rat by intracytoplasmic injection (ICSI) of mutant sperm into oocytes...
November 28, 2016: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/27875264/expression-of-wnt4-5-during-reproductive-cycle-of-catfish-and-wnt5-promoter-analysis
#15
Yarikipati Prathibha, Balasubramanian Senthilkumaran
Signaling molecules, Wnt4 and Wnt5, are essential for ovarian growth during developmental stages in mammals. Although these molecules were identified in several teleosts, their precise expression and role in reproductive processes have not yet been explored in any lower vertebrates. In view of this, using catfish, Clarias batrachus as an animal model, cloning and expression analysis of wnt4 and wnt5 were analyzed in different tissues, at various developmental stages, during ovarian reproductive cycle and after gonadotropin induction...
January 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/27813674/induction-of-tissue-factor-pathway-inhibitor-2-by-hcg-regulates-periovulatory-gene-expression-and-plasmin-activity
#16
Muraly Puttabyatappa, Linah F Al-Alem, Farnosh Zakerkish, Katherine L Rosewell, Mats Brännström, Thomas E Curry
A key event during the process of ovulation is an increased proteolytic activity that aids in breakdown of the follicular wall to permit oocyte release. How the activity of these proteases is regulated is still not known. We hypothesize that Tissue Factor Pathway Inhibitor 2 (TFPI2), a matrix-associated Kunitz-type serine protease inhibitor, plays a role in regulating proteolytic activity during the periovulatory period as in other tissues. TFPI2 is secreted into the extracellular matrix (ECM) where it is postulated to regulate physiological ECM remodeling...
November 4, 2016: Endocrinology
https://www.readbyqxmd.com/read/27796004/ultrastructural-studies-on-oocyte-development-and-vitellogenesis-associated-with-follicle-cells-in-female-scapharca-subcrenata-pelecypoda-arcidae-in-western-korea
#17
Sung Han Kim
Ultrastructural studies on oocyte development and vitellogenesis in oocytes, and the functions of follicle cells during oogenesis and oocyte degeneration were investigated to clarifyb the reproductive mechanism on vitellogenesis of Scapharca subcrenata using electron microscope observations. In this study, vitellogenesis during oogenesis in the oocytes occured by way of autosynthesis and heterosynthesis. Of two processes of vitellogenesis during oogenesis, the process of endogenous autosynthesis involved the combined activity of the Golgi complex, mitochondria and rough endoplasmic reticulum...
September 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/27773884/efficient-mutagenesis-by-crispr-cas-system-during-meiotic-maturation-of-porcine-oocytes
#18
Asuka Onuma, Wataru Fujii, Koji Sugiura, Kunihiko Naito
Genome editing using the CRISPR/Cas system can induce mutations with high efficiency, and allows easier production of genome-modified animals than that offered by the conventional method where embryonic stem cells are used. However, studies using CRISPR/Cas systems have been mostly limited to proliferating somatic cells and pronuclear-stage fertilized eggs. In contrast, the efficiency of a CRISPR/Cas system in immature and maturing oocytes progressing through meiosis has not yet been assessed. In the present study, we evaluated the genome-modification efficiency of the CRISPR/Cas system during meiotic maturation of porcine oocytes...
October 21, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27767095/inhibitory-phosphorylation-of-cdk1-mediates-prolonged-prophase-i-arrest-in-female-germ-cells-and-is-essential-for-female-reproductive-lifespan
#19
Deepak Adhikari, Kiran Busayavalasa, Jingjing Zhang, Mengwen Hu, Sanjiv Risal, Mustafa Bilal Bayazit, Meenakshi Singh, M Kasim Diril, Philipp Kaldis, Kui Liu
A unique feature of female germ cell development in mammals is their remarkably long arrest at the prophase of meiosis I, which lasts up to 50 years in humans. Both dormant and growing oocytes are arrested at prophase I and completely lack the ability to resume meiosis. Here, we show that the prolonged meiotic arrest of female germ cells is largely achieved via the inhibitory phosphorylation of Cdk1 (cyclin-dependent kinase 1). In two mouse models where we have introduced mutant Cdk1(T14AY15F) which cannot be inhibited by phosphorylation (Cdk1AF) in small meiotically incompetent oocytes, the prophase I arrest is interrupted, leading to a premature loss of female germ cells...
November 2016: Cell Research
https://www.readbyqxmd.com/read/27759816/randomized-double-blind-clinical-trial-comparing-two-anesthetic-techniques-for-ultrasound-guided-transvaginal-follicular-puncture
#20
Gilvandro Lins de Oliveira, Fernando Cesar Serralheiro, Fernando Luiz Affonso Fonseca, Onésimo Duarte Ribeiro, Fernando Adami, Denise Maria Christofolini, Bianca Bianco, Caio Parente Barbosa
Objective: To compare the anesthetic techniques using propofol and fentanyl versus midazolam and remifentanil associated with a paracervical block with lidocaine in performing ultrasound-guided transvaginal oocyte aspiration. Methods: A randomized double-blind clinical trial (#RBR-8kqqxh) performed in 61 women submitted to assisted reproductive treatment. The patients were divided into two groups: anesthetic induction with 1mcg/kg of fentanyl associated with 1.5mg/kg of propofol (FP Group, n=32), in comparison with anesthetic induction using 0...
July 2016: Einstein
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