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Oocytes induction

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https://www.readbyqxmd.com/read/28323994/direct-regulation-of-histidine-ammonia-lyase-2-gene-by-thyroid-hormone-in-the-developing-adult-intestinal-stem-cells
#1
Nga Luu, Liezhen Fu, Kenta Fujimoto, Yun-Bo Shi
Most vertebrate organs utilize adult stem cells to maintain homeostasis and ensure proper repair when damaged. How such organ-specific stem cells are formed during vertebrate development is largely unexplored. We have been using the thyroid hormone (T3)-dependent amphibian metamorphosis to address this issue. Early studies in Xenopus laevis have shown that intestinal remodeling involves complete degeneration of the larval epithelium and de novo formation of adult stem cells through dedifferentiation of some larval epithelial cells...
February 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28323970/obesity-modulates-inflammation-and-lipid-metabolism-oocyte-gene-expression-a-single-cell-transcriptome-perspective
#2
Meghan L Ruebel, Matthew Cotter, Clark R Sims, Dean M Moutos, Thomas M Badger, Mario A Cleves, Kartik Shankar, Aline Andres
Context: It is hypothesized that obesity adversely affects the ovarian environment which can disrupt oocyte maturation and may further impact embryonic development. Objective: This study aimed to compare oocyte gene expression profiles and follicular fluid (FF) content from overweight/obese (OW) women and normal weight (NW) women who were undergoing fertility treatments. Design: Using single cell transcriptomic analyses, we investigated oocyte gene expression using RNA-seq...
March 15, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28322775/pregnancy-and-birth-outcomes-in-couples-with-infertility-with-and-without-assisted-reproductive-technology-with-an-emphasis-on-us-population-based-studies
#3
REVIEW
Barbara Luke
Infertility, defined as the inability to conceive within one year of unprotected intercourse, affects an estimated 80 million individuals worldwide, or 10-15 percent of couples of reproductive age. Assisted reproductive technology (ART) includes all infertility treatments to achieve conception; in vitro fertilization (IVF) is the process by which an oocyte is fertilized by semen outside the body; non-IVF ART treatments include ovulation induction, artificial insemination, and intrauterine insemination. Use of ART has risen steadily in the United States during the past two decades due to several reasons, including childbearing at older maternal ages and increasing insurance coverage...
March 18, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28306560/growth-hormone-in-ivf-cycles-any-hope
#4
Roger J Hart, Luk Rombauts, Robert J Norman
PURPOSE OF REVIEW: Growth hormone (GH) has been used as an adjunct in ovulation induction and IVF for 25 years, particularly as an adjunct to ovarian stimulation for women who had a previous poor response to stimulation in an IVF cycle. It does not have US Food and Drug Administration approval for this use. Unfortunately, due to the problems inherent with recruiting women who have undergone unsuccessful IVF treatment cycles and their inevitable low live birth rate per initiated cycle, many studies performed to date have been underpowered...
March 16, 2017: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28265462/fine-selection-of-up-regulated-genes-during-ovulation-by-in-vivo-induction-of-oocyte-maturation-and-ovulation-in-zebrafish
#5
Wanlada Klangnurak, Toshinobu Tokumoto
BACKGROUND: Two essential processes, oocyte maturation and ovulation, are independently induced, but proceed cooperatively as the final step in oogenesis before oocytes become fertilizable. Although these two processes are induced by the same maturation-inducing steroid, 17α, 20β-dihydroxy-4-pregnen-3-one (17, 20β-DHP), in zebrafish, it has been suggested that the receptor, and thus the signal transduction pathway is different for each process. Although much progress has been made in understanding the molecular mechanisms underlying the induction of oocyte maturation, the mechanisms for inducing ovulation remain under investigation...
2017: Zoological Letters
https://www.readbyqxmd.com/read/28257815/gadolinium-released-by-the-linear-gadolinium-based-contrast-agent-gd-dtpa-decreases-the-activity-of-human-epithelial-na-channels-enacs
#6
Fenja Knoepp, Joerg Bettmer, Martin Fronius
BACKGROUND: Gadolinium-based-contrast-agents (GBCAs) are used for magnetic-resonance-imaging and associated with renal and cardiovascular adverse reactions caused by released Gd(3+) ions. Gd(3+) is also a modulator of mechano-gated ion channels, including the epithelial Na(+) channel (ENaC) that is expressed in kidney epithelium and the vasculature. ENaC is important for salt-/water homeostasis and blood pressure regulation and a likely target of released Gd(3+) from GBCAs causing the above-mentioned adverse reactions...
February 28, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28253290/transcriptomic-features-of-pecten-maximus-oocyte-quality-and-maturation
#7
Marianna Pauletto, Massimo Milan, Arnaud Huvet, Charlotte Corporeau, Marc Suquet, Josep V Planas, Rebeca Moreira, Antonio Figueras, Beatriz Novoa, Tomaso Patarnello, Luca Bargelloni
The king scallop Pecten maximus is a high valuable species of great interest in Europe for both fishery and aquaculture. Notably, there has been an increased investment to produce seed for enhancement programmes of wild scallop populations. However, hatchery production is a relatively new industry and it is still underdeveloped. Major hurdles are spawning control and gamete quality. In the present study, a total of 14 scallops were sampled in the bay of Brest (Brittany, France) to compare transcriptomic profiles of mature oocytes collected by spawning induction or by stripping...
2017: PloS One
https://www.readbyqxmd.com/read/28243539/inhibition-of-calcineurin-by-fk506-stimulates-germinal-vesicle-breakdown-of-mouse-oocytes-in-hypoxanthine-supplemented-medium
#8
Li Wang, Yan-Hong Zhen, Xiao-Ming Liu, Jing Cao, Yan-Ling Wang, Li-Jun Huo
Calcineurin (CN) is a serine/threonine phosphatase which plays important roles in meiosis maturation in invertebrate oocytes; however, the role of CN in mouse oocytes is relatively unexplored. In this study, we examined the expression, localization and functional roles of CN in mouse oocytes and granulosa cells. The RT-PCR results showed that the β isoform of calcineurin A subunit (Cn A) expressed significantly higher than α and γ isoforms, and the expression of Cn Aβ mRNA obviously decreased in oocytes in which germinal vesicle breakdown (GVBD) occurred, while only B1 of calcineurin B subunit (Cn B) was detected in oocytes and stably expressed during oocytes maturation...
2017: PeerJ
https://www.readbyqxmd.com/read/28239035/characterization-of-membrane-progestin-receptor-%C3%AE-mpr%C3%AE-of-the-medaka-and-role-in-the-induction-of-oocyte-maturation
#9
Shimi Rani Roy, Jun Wang, Md Rubel Rana, Mikiko Nakashima, Toshinobu Tokumoto
Oocyte maturation in medaka is induced by the maturation-inducing hormone (MIH) via its membrane receptor. The most likely candidates for the membrane receptor are membrane progestin receptors (mPRs). In order to characterize the mPRα subtype of medaka, a human cell line expressing the mPRα gene of medaka was established and its steroid binding property was assessed. The α subtype exhibited high binding affinity for 17,20β-DHP, the MIH in medaka. Treatment with a morpholino antisense oligonucleotide to mPRα blocked oocyte maturation in vivo...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28193316/polydatin-improves-the-developmental-competence-of-bovine-embryos-in-vitro-via-induction-of-sirtuin-1-sirt1
#10
Imran Khan, Sung Woo Kim, Kyung-Lim Lee, Seok-Hwan Song, Ayman Mesalam, M M R Chowdhury, Zia Uddin, Ki Hun Park, Il-Keun Kong
The aim of the present study was to investigate the beneficial effect of polydatin (PD), the glycoside form of resveratrol, on embryo development in vitro. Oocytes were aspirated from ovaries of Korean Hanwoo cows and cultured until Day 8 in a humidified atmosphere of 5% CO2 in air at 38.5°C. Protein and gene expression levels were determined through confocal microscopy and reverse transcription-polymerase chain reaction respectively, whereas the number of total and apoptotic cells in Day 8 blastocysts was determined using Hoechst 33342 staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling...
February 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28185602/impaired-hypothalamic-pituitary-testicular-axis-activity-spermatogenesis-and-sperm-function-promote-infertility-in-males-with-lead-poisoning
#11
Jason Gandhi, Rafael J Hernandez, Andrew Chen, Noel L Smith, Yefim R Sheynkin, Gargi Joshi, Sardar Ali Khan
Lead poisoning is a stealthy threat to human physiological systems as chronic exposure can remain asymptomatic for long periods of time before symptoms manifest. We presently review the biophysical mechanisms of lead poisoning that contribute to male infertility. Environmental and occupational exposure of lead may adversely affect the hypothalamic-pituitary-testicular axis, impairing the induction of spermatogenesis. Dysfunction at the reproductive axis, namely testosterone suppression, is most susceptible and irreversible during pubertal development...
February 10, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28144637/induction-of-fas-mediated-apoptosis-by-interferon-%C3%AE-is-dependent-on-granulosa-cell-differentiation-and-follicular-maturation-in-the-rat-ovary
#12
Hye-Jeong Lee, Ji Young Kim, Ji Eun Park, Yong-Dal Yoon, Benjamin K Tsang, Jong-Min Kim
Fas ligand (FasL) and its receptor Fas have been implicated in granulosa cell apoptosis during follicular atresia. Although interferon-gamma (IFN-γ) is believed to be involved in the regulation Fas expression in differentiated granulosa or granulosa-luteal cells, the expression of this cytokine and its role in the regulation of the granulosa cell Fas/FasL system and apoptosis during follicular maturation have not been thoroughly investigated. In the present study, we have examined the presence of IFN-γ in ovarian follicles at different stage of development by immunohistochemistry and related their relative intensities with follicular expression of Fas and FasL, and with differences in granulosa cell sensitivity to Fas activation by exogenous agonistic Anti-Fas monoclonal antibody (Fas mAb)...
December 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28112985/comparison-of-human-amniotic-chorionic-and-umbilical-cord-multipotent-mesenchymal-stem-cells-regarding-their-capacity-for-differentiation-toward-female-germ-cells
#13
Hamid Reza Asgari, Mohammad Akbari, Hossein Yazdekhasti, Zahra Rajabi, Shadan Navid, Fereshte Aliakbari, Niloufar Abbasi, Freidon Sargolzaei Aval, Alireza Shams, Mehdi Abbasi
Placenta harbors a plentiful source of various cells with stem cells or stem-like cell properties, which can be used in therapeutic procedures and research. Mesenchymal stem cells (MSCs) have attracted much attention due to their specific differentiation potential and tolerogenic properties. MSCs have been isolated from different parts of placenta; however, in this study, we isolated MSCs from amnion and chorion membrane, as well as umbilical cord (Wharton's jelly [WJ]) and compared their capacity regarding differentiation toward female germ cells under influence of 10 ng/mL BMP4...
February 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28070808/combined-positive-effect-of-oocyte-extracts-and-brilliant-cresyl-blue-stained-recipient-cytoplasts-on-epigenetic-reprogramming-and-gene-expression-in-buffalo-nuclear-transfer-embryos
#14
E M Sadeesh, Shah Fozia, Kataria Meena
This study examined the effects of buffalo oocyte extracts (BOE) on donor cells reprogramming and molecular characterisation of oocytes screened via brilliant cresyl blue (BCB) staining and comparison of gene expression profiles of developmentally important genes in blastocysts from IVF and cloned derived from BOE treated donor cells with BCB selected recipient cytoplasts. Relative abundance (RA) of OCT4 and NANOG was increased (P < 0.05) and HDAC-1, DNMT-1, and DNMT-3A decreased (P < 0.05) in extract treated cells (ETCs)...
January 9, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28069903/improvement-of-in-vitro-produced-bovine-embryo-treated-with-coa-a-under-heat-stressed-condition
#15
Imran Khan, Kyeong-Lim Lee, Lianguang Xu, Ayman Mesalam, Mmr Chowdhury, Myeong-Don Joo, Ihsan-Ul- Haq, Bushra Mirza, Il-Keun Kong
Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during in vitro production of bovine embryos. Oocytes were incubated in in vitro maturation (IVM) media with or without coa-A at two different temperatures, 40.5 °C and 42 °C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5 °C (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-kB) and cyclooxygenase-2 (COX2)...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28050961/final-oocyte-maturation-in-assisted-reproduction-with-human-chorionic-gonadotropin-and-gonadotropin-releasing-hormone-agonist-dual-trigger
#16
REVIEW
Sofia Andrade de Oliveira, Vinícius Fernando Calsavara, Gemma Castillón Cortés
Final oocyte maturation with Human Chorionic Gonadotropin (hCG) and ovarian stimulation with Follicle Stimulation Hormone (FSH) combined with Gonadotrophin-releasing Hormone (GnRH) antagonist to block Luteinizing hormone (LH) surge is a standard procedure of in vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI). However, GnRH agonist has been replacing the use of hCG in certain situations, especially in patients at risk of Ovarian Hyperstimulation Syndrome (OHSS). Some studies have also shown advantages in the combined use of GnRH agonist concurrently with hCG in inducing final oocyte maturation, a treatment known as "Dual Trigger"...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28043371/sperm-bound-antisperm-antibodies-prevent-capacitation-of-bovine-spermatozoa
#17
M S Ferrer, J Klabnik-Bradford, D E Anderson, A C Bullington, R A Palomares, L M J Miller, R Stawicki, M Miesner
It was hypothesized here that sperm-bound antisperm antibodies (ASAs) impair the ability of bovine spermatozoa to undergo capacitation, bind to the zona pellucida, and complete the acrosome reaction. The effect of ASA binding on these functions was evaluated in frozen/thawed spermatozoa from four bulls before and after induction of ASAs. Ejaculates were divided into ASA negative (<10% immunoglobulin [Ig]G- and IgA-bound spermatozoa) or ASA positive (≥20% IgG and/or IgA-bound spermatozoa). The percentage of capacitated (Merocyanine 540 positive) live spermatozoa in response to heparin was lower in ASA-positive than ASA-negative ejaculates (P < 0...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/27998857/-clinical-outcome-of-in-vitro-fertilization-or-intracytoplasmic-sperm-injection-embryo-transfer-in-women-aged-40-years-and-above
#18
Xing-Yu Zhou, Shu-Xian Feng, Xue-Lan Li, Yu-Dong Liu, Xin Chen, De-Sheng Ye, Jing Niu, Shi-Ling Chen
OBJECTIVE: To investigate the clinical outcomes in vitro fertilization or intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) in women aged over 40 years. METHODS: We retrospectively analyzed 1050 non-donor IVF/ICSI-ET cycles performed from January, 2007 to December, 2015 in women at the age 40 years or above, including 393 women at 40 years of age, 266 at 41 years, 158 at 42 years, 107 at 43 years, 64 at 44 years, and 65 at 45-51 years. The clinical characteristics and outcomes of the women in different age groups were compared and analyzed...
December 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27988256/oocyte-competence-in-women-with-polycystic-ovary-syndrome
#19
REVIEW
Stefano Palomba, Jessica Daolio, Giovanni Battista La Sala
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age and affects fertility and pregnancy in cases of oligoanovulation. Ovulation induction is often used to treat anovulatory patients with PCOS, but many of these women fail to conceive and resort to assisted reproductive technologies. Alterations in oocyte competence (OC) are considered potential causative factors for subfertility in women with PCOS. In this review we present and critically assess all recent clinical and experimental data regarding OC in women with PCOS...
March 2017: Trends in Endocrinology and Metabolism: TEM
https://www.readbyqxmd.com/read/27974271/silver-nanoparticles-induce-oocyte-maturation-in-zebrafish-danio-rerio
#20
Shi Xi Chen, Xiao Zhen Yang, Ying Deng, Jing Huang, Yan Li, Qian Sun, Chang-Ping Yu, Yong Zhu, Wan Shu Hong
Public concern regarding silver nanoparticles (AgNPs) in the environment has been increasing since they can cause adverse effects in some aquatic species. However, few data are actually available on the effects of AgNPs on the germ cells. In the present study, we used the zebrafish ovarian follicle as a model to assess the potentially adverse effects of AgNPs on oocyte maturation (germinal vesicle breakdown, GVBD) in vitro. Similar to the maturation inducing hormone (17α, 20β-dihydroxy-4-pregnen-3-one), AgNPs induced GVBD, and reduced the total cyclic adenosine monophosphate (cAMP) concentration in zebrafish ovarian follicles...
March 2017: Chemosphere
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