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Tianci Huang, Qi Yu, Shujuan Liu, Wei Huang, Qiang Zhao
This article highlights the utilization of phosphorescent iridium(iii) complexes for biosensing and photodynamic therapy. Comments on the effectiveness of these complexes in biological applications are included. Challenges and potential solutions are also discussed for further development and exploitation.
April 25, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Nadia Peerboom, Eneas Schmidt, Edward Trybala, Stephan Block, Tomas Bergström, Hudson P Pace, Marta Bally
Discovery and development of new antiviral therapies essentially rely on two key factors: an in-depth understanding of the mechanisms involved in viral infection and the development of fast and versatile drug screening platforms. To meet those demands, we present a biosensing platform to probe virus-cell membrane interactions on a single particle level. Our method is based on the formation of supported lipid bilayers from cell membrane material. Using total internal reflection fluorescence microscopy, we report the contribution of viral and cellular components to the interaction kinetics of herpes simplex virus type 1 with the cell membrane...
April 24, 2018: ACS Infectious Diseases
Thomas R Carey, Kristen L Cotner, Brian Li, Lydia L Sohn
Advancements in microfluidic technologies have led to the development of many new tools for both the characterization and sorting of single cells without the need for exogenous labels. Label-free microfluidics reduce the preparation time, reagents needed, and cost of conventional methods based on fluorescent or magnetic labels. Furthermore, these devices enable analysis of cell properties such as mechanical phenotype and dielectric parameters that cannot be characterized with traditional labels. Some of the most promising technologies for current and future development toward label-free, single-cell analysis and sorting include electronic sensors such as Coulter counters and electrical impedance cytometry; deformation analysis using optical traps and deformation cytometry; hydrodynamic sorting such as deterministic lateral displacement, inertial focusing, and microvortex trapping; and acoustic sorting using traveling or standing surface acoustic waves...
April 24, 2018: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
Qianqian Zhu, Wei Zhuang, Yong Chen, Zhanke Wang, Byron Villacorta Hernandez, Jinglan Wu, Pengpeng Yang, Dong Liu, Chenjie Zhu, Hanjie Ying, Zhonghua Zhu
In order to improve the stability and recyclability of enzymes immobilized on metal organic framewoks (MOFs), graphene oxide (GO) with surface oxygen-rich functional groups was selected to form ZIF-8/GO nanocomposites with the zeolitic imidazolate framework (ZIF-8) for Cytochrome c (Cyt c) immobilization. It was found that functional groups on GO surface were involved in the growth of ZIF-8 without affecting the crystal structure, but their particle sizes reduced to about 200 nm. The storage stability and resistance toward to organic solvents of Cyt c were obviously improved after immobilization on the ZIF-8/GO nanocomposite...
April 24, 2018: ACS Applied Materials & Interfaces
Huan Pang, Yang Zang, Jing Fan, Ju Yun, Huai Guo Xue
Photoelectrochemical (PEC) analysis is a new detection technique developed in recent years, which has the advantages of high sensitivity, low background signal and desirable selectivity, obtaining the great progress in sensing applications. Semiconductor nanomaterials with excellent photoelectric activity have played a vital role in the construction of PEC sensing platform. Thus, this review introduces the recent advances of semiconductor nanomaterials-based PEC analysis, and describes the typical PEC sensing strategies...
April 24, 2018: Chemistry: a European Journal
Eloy Povedano, Eva Vargas, Víctor Ruiz-Valdepeñas Montiel, Rebeca M Torrente-Rodríguez, María Pedrero, Rodrigo Barderas, Pablo San Segundo-Acosta, Alberto Peláez-García, Marta Mendiola, David Hardisson, Susana Campuzano, José M Pingarrón
This paper describes two different electrochemical affinity biosensing approaches for the simple, fast and bisulfite and PCR-free quantification of 5-methylated cytosines (5-mC) in DNA using the anti-5-mC antibody as biorecognition element. One of the biosensing approaches used the anti-5-mC as capture bioreceptor and a sandwich type immunoassay, while the other one involved the use of a specific DNA probe and the anti-5-mC as a detector bioreceptor of the captured methylated DNA. Both strategies, named for simplicity in the text as immunosensor and DNA sensor, respectively, were implemented on the surface of magnetic microparticles and the transduction was accomplished by amperometry at screen-printed carbon electrodes by means of the hydrogen peroxide/hydroquinone system...
April 23, 2018: Scientific Reports
Ghobad Mohammadi, Elahe Faramarzi, Majid Mahmoudi, Sirous Ghobadi, Ali Reza Ghiasvand, Hector C Goicoechea, Ali R Jalalvand
In this work, voltammetric data recorded by a glassy carbon electrode (GCE) was used to investigate the interactions of tolcapone (Tasmar, TAS) with human serum albumin (HSA) at the electrode surface. The recorded voltammetric data was also combined with spectroscopic data to construct an augmented data matrix which was analysed by multivariate curve resolution-alternating least squares (MCR-ALS) as an efficient chemometric tool to obtain more information about TAS-HSA interactions. The results of MCR-ALS confirmed formation of one complex species (HSA-TAS2 ) and application of MCR-BANDS to the results of MCR-ALS confirmed the absence of rotational ambiguities and existing unambiguous and reliable results...
April 17, 2018: Journal of Pharmaceutical and Biomedical Analysis
Chunxiu Liu, Hao Yu, Qiang Li, Changqing Zhu, Yunsheng Xia
The exploration of fluorescent tools with distinguished optical properties and favorable biocompatibility is significant for bio-sensing and bio-imaging. We herein present a host-guest interactions aided strategy for fabricating fluorescent silica nanoparticles (FSNPs), which is enabled by cyclodextrin (CD) supermolecules. Compared with conventional FSNPs, the modified products (are named as fluorophore@CD@SNPs) possess several advantages. First, the incorporated fluorophores can thoroughly get rid of their intrinsic aggregation due to CD's inclusion effect, and the fluorescence intensity of the obtained fluorophore@CD@SNPs can enhance 48-67%...
April 23, 2018: ACS Applied Materials & Interfaces
Yongfei Li, Lei Song, Bang Wang, Jianbo He, Yulin Li, Zhaoxiang Deng, Chengde Mao
pH-responsivity has been widely pursued in dynamic DNA nanotechnology, due to its great potential in biosensing, controlled release and nanomachinery. Previously developed pH-triggering systems mostly depend on specific designs of DNA sequences. However, sequence-independent regulation could provide a more general tool to achieve pH-responsive DNA assembly, which has yet to be developed. Herein, we propose a mechanism for dynamic DNA assembly by utilizing ethylenediamine (EN) as a reversibly chargeable (via protonation) cation to overcome electrostatic repulsions...
April 23, 2018: Angewandte Chemie
Sahin Buyukdagli
The optimal functioning of nanopore-based biosensing tools necessitates rapid polymer capture from the ion reservoir. We identify an ionic correlation-induced transport mechanism that provides this condition without the chemical modification of the polymer or the pore surface. In the typical experimental configuration where a negatively charged silicon-based pore confines a 1 : 1 electrolyte solution, anionic polymer capture is limited by electrostatic polymer-membrane repulsion and the electroosmotic (EO) flow...
April 23, 2018: Soft Matter
Rohit Chand, Saipriya Ramalingam, Suresh Neethirajan
Nanoscale MoS2 has attracted extensive attention for sensing due to its superior properties. This study outlines a microfluidic and electrochemical biosensing methodology for the multiplex detection of paratuberculosis-specific miRNAs. Herein, we report the synthesis of MoS2 nanosheets decorated with a copper ferrite (CuFe2O4) nanoparticle composite and molecular probe immobilized MoS2 nanosheets as nanocarriers for the electrochemical detection of miRNAs. Paratuberculosis is a bacterial infection of the intestinal tract of dairy cattle, and is a cause of substantial economic and animal losses all over the world...
April 23, 2018: Nanoscale
Jin-Xiang Zeng, Juan Wang, Shou-Wen Zhang, Ji-Xiao Zhu, Min Li, Wei-Hua Huang, Jin-Yi Wan, Hai-Qiang Yao, Chong-Zhi Wang, Chun-Su Yuan
The XOD inhibitory effects of Plantaginis Semen, that is, the seeds of P. asiatisca , and its representative four single compounds, acteoside, 1H-indolo-3-carbaldehyde, isoacteoside, and myristic acid, were evaluated by electron transfer signal blocking activities (ETSBA), which is based on the electron transfer signal of XOD enzymatic reaction. The blocking activities were detected using an electrochemical biosensing method. Compared with control, significant effects were observed after the addition of P. asiatica extract, acteoside, and 1H-indolo-3-carbaldehyde (all p < 0...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Yanfang He, Fei Qi, Xiangheng Niu, Wenchi Zhang, Xifeng Zhang, Jianming Pan
In clinical diagnosis, monitoring of uric acid (UA) is generally realized by combining uricase with natural peroxidase. The use of bio-enzymes, however, shadows some highlights of these methods due to their vulnerable activities against environments. Herein, we report a novel biosensor for the natural enzyme-free colorimetric detection of UA by using CoP nanosheet arrays grown on Ni foam (NF) as a monolithic peroxidase mimic. The integrated nanozyme can be put into and taken out from reaction systems conveniently with only tweezers, making it possible for on-demand analysis...
August 27, 2018: Analytica Chimica Acta
Julian Bello, Jiwook Shim
Nanopore fabrication via the controlled dielectric breakdown (CDB) method offers an opportunity to create solid-state nanopores directly in salt solution with sub-nanometer precision. Driven by trap assisted current tunneling, the method uses localized defects, or traps, in the dielectric material to isolate a breakdown point and fabricate a single pore in less than 10 minutes. Here we present an approach to controlled dielectric breakdown of SiNx in which the nanopore is fabricated in LiCl buffer instead of the traditional KCl buffer...
April 21, 2018: Biomedical Microdevices
Laura Moro, Gianni Pezzotti, Mehmet Turemis, Josep Sanchís, Marinella Farré, Renata Denaro, Maria Grazia Giacobbe, Francesca Crisafi, Maria Teresa Giardi
The present study evaluates an optical bioassay based on green photosynthetic microalgae as a promising alternative for monitoring of relevant seawater pollutants. Photosystem II fluorescence parameters from several microalgae species were examined in the presence of three common marine pesticides that act as photosynthesis inhibitors. The three pollutants were detected within 10 min in concentrations between ng/L-μg/L. The different algae species showed slightly diverse pesticide sensitivities, being Chlorella mirabilis the most sensitive one...
April 2018: Marine Pollution Bulletin
Elkin Lopez-Fontal, Anna Grochmal, Tom Foran, Lilia Milanesi, Salvador Tomas
Understanding self-assembly in confined spaces is essential to fully understand molecular processes in confined cell compartments and will offer clues on the behaviour of simple confined systems, such as protocells and lipid-vesicle based devices. Using a model system composed of lipid vesicles, a membrane impermeable receptor and a membrane-permeable ligand, we have studied in detail how compartmentalization modulates the interaction between the confined receptor and its ligand. We demonstrate that confinement of one of the building blocks stabilizes complex self-assembled structures to the extent that dilution leads, counterintuitively, to the formation of long range assemblies...
February 21, 2018: Chemical Science
Marcos Eguílaz, Reynaldo Villalonga, Gustavo Rivas
We are reporting a novel biosensing platform based on a hybrid nanomaterial that combines the advantages of Nafion-coated multiwalled carbon nanotubes (MWCNTs) and mesoporous silica MCM41 nanoparticles functionalized with hemoglobin (Hb). MWCNTs-MCM41-Hb hybrid bioconjugate was characterized by scanning electron microscopy (SEM), UV-vis spectroscopy and electrochemical techniques after deposition at glassy carbon electrodes (GCE). The combination of the high surface area, biocompatibility and protein loading capacity of MCM41 nanoparticles and the high surface area and catalytic properties of MWCNTs allowed the direct electron transfer (DET) between Hb and the electrode surface...
April 4, 2018: Biosensors & Bioelectronics
Claire Chattaway, Sabrina Belbekhouche, Filip E Du Prez, Karine Glinel, Sophie Madeleine Demoustier-Champagne
The development of multifunctional surfaces is of general interest for the fabrication of biomedical, catalytic, microfluidic or biosensing devices. Herein, we report on the preparation of copolymer layers immobilized on gold surface and showing both free thiol and amino groups. These layers are produced by aminolysis of a thiolactone-based copolymer in the presence of a diamine, according to a one-step procedure. The free thiol and amino groups present in the modified copolymer layers can be successfully functionalized with respectively thiolated and carboxylic derivatives, in order to produce bifunctionalized surfaces...
April 19, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
YoungTae Seo, Sunil Jeong, JuKyung Lee, Hak Soo Choi, Jonghan Kim, HeaYeon Lee
Nanostructured biosensors have pioneered biomedical engineering by providing highly sensitive analyses of biomolecules. The nanowell array (NWA)-based biosensing platform is particularly innovative, where the small size of NWs within the array permits extremely profound sensing of a small quantity of biomolecules. Undoubtedly, the NWA geometry of a gently-sloped vertical wall is critical for selective docking of specific proteins without capillary resistances, and nanoprocessing has contributed to the fabrication of NWA electrodes on gold substrate such as molding process, e-beam lithography, and krypton-fluoride (KrF) stepper semiconductor method...
2018: Nano Convergence
Nobuo Misawa, Toshihisa Osaki, Shoji Takeuchi
This review highlights recent development of biosensors that use the functions of membrane proteins. Membrane proteins are essential components of biological membranes and have a central role in detection of various environmental stimuli such as olfaction and gustation. A number of studies have attempted for development of biosensors using the sensing property of these membrane proteins. Their specificity to target molecules is particularly attractive as it is significantly superior to that of traditional human-made sensors...
April 2018: Journal of the Royal Society, Interface
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