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https://www.readbyqxmd.com/read/28342377/live-cell-biosensing-platforms-using-graphene-based-hybrid-nanomaterials
#1
REVIEW
Tae-Hyung Kim, Donghyun Lee, Jeong-Woo Choi
A novel strategy to precisely detect or monitor various biomaterials in living cells poses paramount importance in understanding cellular processes. Graphene, a newly emerged two-dimensional carbon material, has been widely utilized for biosensors owing to its multifarious characteristics including mechanical, electrical, and optical properties (e.g. stability, conductivity, fluorescence quenching and photoluminescence). In addition, graphene derivatives and their innate characteristics, such as biocompatibility low cytotoxicity and water solubility have facilitated the use of graphene-based materials for live cell biosensing, wherein graphene is utilized as a core material by itself or in combination with other functional nanomaterials to load target-specific probes, fluorescent dyes, and other signaling molecules...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28337919/new-insights-towards-efficient-charge-separation-mechanism-for-high-performances-photoelectrochemical-aptasensing-enhanced-charge-carriers-lifetime-via-coupling-ultrathin-mos2-nanoplates-with-nitrogen-doped-graphene-quantum-dots
#2
Ding Jiang, Xiaojiao Du, Lei Zhou, Henan Li, Kun Wang
Deeply understanding the internal mechanism of photoelectrohemical (PEC) process is conducive to fabricate high-performances PEC biosensors. In this work, we proposed a new insight towards efficient charge separation mechanism in high-performances PEC biosensors. Specifically, we disclosed that the lifetimes of photogenerated charge carriers of ultrathin MoS2 nanosheets could be prolonged by approximately millisecond timescales after a proper mole ratio of NGQDs were coupled, leading to the promoted charge separation, and giant photocurrent signal-magnify...
March 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28335185/nanomaterials-for-biosensing-applications
#3
EDITORIAL
Sichao Hou, Aiying Zhang, Ming Su
Nanomaterials have shown tremendous potentials to impact the broad field of biological sensing. Nanomaterials, with extremely small sizes and appropriate surface modifications, allow intimate interaction with target biomolecules. [...].
March 30, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28332478/addressed-immobilization-of-biofunctionalized-diatoms-on-electrodes-by-gold-electrodeposition
#4
S Leonardo, D Garibo, M Fernández-Tejedor, C K O'Sullivan, M Campàs
Diatoms are single cell microalgae with a silica shell (frustule), which possess a micro/nanoporous pattern of unparalleled diversity far beyond the possibilities of current micro- and nanofabrication techniques. To explore diatoms as natural three-dimensional nanostructured supports in sensing and biosensing devices, a simple, rapid and stable method to immobilize diatoms via gold electrodeposition is described. In this process, gold microstructures are formed, immobilizing diatoms by entrapment or crossing their nanopores...
March 23, 2017: Biofabrication
https://www.readbyqxmd.com/read/28325036/microwave-hydrothermal-synthesis-of-terbium-ions-complexed-with-porous-graphene-for-effective-absorbent-for-organic-dye
#5
Keqin Chen, Hui Gao, Bowei Bai, Wenjing Liu, Xiaolong Li
A luminescent terbium ions/reduced graphene oxide complex (Tb-RGO) was successfully and rapidly synthesized by the microwave hydrothermal reaction via the interactions between terbium ions and the active oxygen functional groups of graphene oxide. The as-prepared material was porous stacked by multilayer graphene in all directions. Thus, the resulting product owed the high specific surface area, high adsorption capacity and ultra-fast adsorption rate. Combined with the characteristic photoluminescence derived from terbium ions, the material has potential applications in biosensing and environmental protection...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28319906/functional-characterization-of-gram-negative-bacteria-from-different-genera-as-multiplex-cadmium-biosensors
#6
Lara Bereza-Malcolm, Sanja Aracic, Ruban Kannan, Gülay Mann, Ashley E Franks
Widespread presence of cadmium in soil and water systems is a consequence of industrial and agricultural processes. Subsequent accumulation of cadmium in food and drinking water can result in accidental consumption of dangerous concentrations. As such, cadmium environmental contamination poses a significant threat to human health. Development of microbial biosensors, as a novel alternative method for in situ cadmium detection, may reduce human exposure by complementing traditional analytical methods. In this study, a multiplex cadmium biosensing construct was assembled by cloning a single-output cadmium biosensor element, cadRgfp, and a constitutively expressed mrfp1 onto a broad-host range vector...
March 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28318819/slow-pathway-radiofrequency-ablation-using-magnetic-navigation-a-description-of-technique-and-retrospective-case-analysis
#7
Abhishek Bhaskaran, Maha Albarri, Neil Ross, Sara Al Raisi, Rahul Samantha, Leonette Roode, Fazlur Nadri, Jeanette Ng, Stuart Thomas, Aravinda Thiagalingam, Pramesh Kovoor
BACKGROUND: The Magnetic Navigation System (MNS) catheter was shown to be stable in the presence of significant cardiac wall motion and delivered more effective lesions compared to manual control. This stability could potentially make AV junctional reentrant tachycardia (AVNRT) ablation safer. The aim of this study is to describe the method of mapping and ablation of AVNRT with MNS and 3-D electro-anatomical mapping system (CARTO, Biosense Webster, Diamond bar, CA, USA) anatomical mapping, with a view to improve the safety of ablation...
February 21, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28317969/yolk-shell-nanostructured-fe3o4-c-magnetic-nanoparticles-with-enhanced-peroxidase-like-activity-for-label-free-colorimetric-detection-of-h2o2-and-glucose
#8
Na Lu, Min Zhang, Lei Ding, Jing Zheng, Caixia Zeng, Yanli Wen, Gang Liu, Ali Aldalbahi, Jiye Shi, Shiping Song, Xiaolei Zuo, Lihua Wang
Herein, we have developed a simple and facile method to synthesize yolk-shell nanostructured Fe3O4@C nanoparticles (NPs) as a multifunctional biosensing platform for the label-free colorimetric detection of H2O2 and glucose. It was demonstrated that Fe3O4@C yolk-shell nanostructures (YSNs) retained the magnetic properties that can be used for separation and concentration. Also importantly, the Fe3O4@C YSNs exhibited an intrinsic peroxidase-like activity that could quickly catalyze the enzyme substrate in the presence of H2O2 and produce a blue color...
March 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28316108/cry1a-b-16-toxin-from-bacillus-thuringiensis-theoretical-refinement-of-three-dimensional-structure-and-prediction-of-peptides-as-molecular-markers-for-detection-of-genetically-modified-organisms
#9
Alexandra Plácido, Andreia Coelho, Lucas Abreu do Nascimento, Andreanne Gomes Vasconcelos, Maria Fátima Barroso, Joilson Ramos-Jesus, Vladimir Costa, Francisco das Chagas Alves Lima, Cristina Delerue-Matos, Ricardo Martins Ramos, Mariela M Marani, José Roberto de Souza de Almeida Leite
Transgenic maize produced by the insertion of the Cry transgene into its genome became the second most cultivated crop worldwide. Cry gene from Bacillus thuringiensis kurstaki expresses protein derivatives of crystalline endotoxins which confer insect resistance onto the maize crop. Mandatory labeling of processed food containing or made by genetically modified organisms is in force in many countries, so, it is very urgent to develop fast and practical methods for GMO identification, e.g., biosensors. In the absence of an available empirical structure of Cry1A(b)16 protein, a theoretical model was effectively generated, in this work, by homology modeling and molecular dynamics simulations based on two available homologous protein structures...
March 18, 2017: Proteins
https://www.readbyqxmd.com/read/28314426/erratum-to-nanocrystals-of-zeolite-act-as-enhanced-sensing-interface-for-biosensing-of-leviteracetum
#10
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28314279/comparative-analysis-of-a-fret-based-plk1-kinase-assay-to-identify-plk1-inhibitors-for-chemotherapy
#11
Sol-Bi Shin, Sang-Uk Woo, Young-Joo Lee, Hyungshin Yim
Advanced techniques for detecting kinase inhibitors are in demand due to limitations of traditional approaches. Here, we used a fluorescence resonance energy transfer (FRET)-based kinase assay, a sensitive fluorescence turn-on biosensing platform, to identify a Polo-like kinase 1 (PLK1) inhibitor. The assay was developed with the Z'-Lyte™ FRET-peptide and PLK1 kinase purified from a baculovirus expression system. Using PLK1 inhibitors, sensitivity and efficiency of this FRET-based PLK1 kinase assay were compared to those of radioisotope-based and immunoblot-based assays...
March 2017: Anticancer Research
https://www.readbyqxmd.com/read/28301751/plasmonic-imaging-of-electrochemical-impedance
#12
Liang Yuan, Nongjian Tao, Wei Wang
Electrochemical impedance spectroscopy (EIS) measures the frequency spectrum of an electrochemical interface to resist an alternating current. This method allows label-free and noninvasive studies on interfacial adsorption and molecular interactions and has applications in biosensing and drug screening. Although powerful, traditional EIS lacks spatial resolution or imaging capability, hindering the study of heterogeneous electrochemical processes on electrodes. We have recently developed a plasmonic-based electrochemical impedance technique to image local electrochemical impedance with a submicron spatial resolution and a submillisecond temporal resolution...
March 6, 2017: Annual Review of Analytical Chemistry
https://www.readbyqxmd.com/read/28301164/effect-of-meso-vs-macro-size-of-hierarchical-porous-silica-on-the-adsorption-and-activity-of-immobilized-%C3%AE-galactosidase
#13
Ileana-Alexandra Pavel, Sofia F Prazeres, Gemma Montalvo, Carmen Garcia-Ruiz, Vincent Nicolas, Alain Celzard, Francois Dehez, Laetitia Canabady-Rochelle, Nadia Canilho, Andreea Pasc
β-galactosidase (β-Gal) is one of the most important enzymes used in milk processing for improving their nutritional quality and digestibility. Herein, β-Gal has been entrapped into a meso-macroporous material (average pore size 9 and 200 nm, respectively) prepared by a sol-gel method from a silica precursor and a dispersion of solid lipid nanoparticles in a micelle phase. The physisorption of the enzyme depends on the concentration of the feed solution and on the pore size of the support. The enzyme is preferentially adsorbed either in mesopores or in macropores, depending on its initial concentration...
March 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28300246/pore-modulation-of-zirconium-organic-frameworks-for-high-efficiency-detection-of-trace-proteins
#14
Chun-Sen Liu, Zhi-Hong Zhang, Min Chen, Hui Zhao, Feng-He Duan, Di-Ming Chen, Ming-Hua Wang, Shuai Zhang, Miao Du
Tunable electrochemical biosensors based on analogous Zr-MOFs were developed for protein detection, the performances of which rely on the pore sizes and surroundings of the MOFs that show diverse binding behaviors to aptamers and then the targeted proteins. The optimized Zr-MOF-based sensor has high selectivity to lysozyme in a wide concentration range and a low detection limit of 3.6 pg mL(-1), with good repeatability, stability, and applicability in real samples. This work will establish a new platform for biosensing and extend the application scope of MOF materials...
March 16, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28299937/non-covalent-protein-and-peptide-functionalization-of-single-walled-carbon-nanotubes-for-bio-delivery-and-optical-sensing-applications
#15
Alessandra Antonucci, Justyna Kupis-Rozmyslowicz, Ardemis Boghossian
The exquisite structural and optical characteristics of single-walled carbon nanotubes (SWCNTs), combined with the tunable specificities of proteins and peptides, can be exploited to strongly benefit technologies with applications in fields ranging from biomedicine to industrial biocatalysis. The key to exploiting the synergism of these materials is designing protein/peptide-SWCNT conjugation schemes that preserve biomolecule activity while keeping the near-infrared optical and electronic properties of SWCNTs intact...
March 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28299707/functionalized-vesicles-by-microfluidic-device
#16
Derek Vallejo, Shih-Hui Lee, Abraham Lee
In recent years, lipid vesicles have become popular vehicles for the creation of biosensors. Vesicles can hold reaction components within a selective permeable membrane that provides an ideal environment for membrane protein biosensing elements. The lipid bilayer allows a protein to retain its native structure and function, and the membrane fluidity can allow for conformational changes and physiological interactions with target analytes. Here, we present two methods for the production of giant unilamellar vesicles (GUVs) within a microfluidic device that can be used as the basis for a biosensor...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28299695/development-of-novel-piezoelectric-biosensor-using-pzt-ceramic-resonator-for-detection-of-cancer-markers
#17
Li Su, Chi-Chun Fong, Pik-Yuan Cheung, Mengsu Yang
A novel biosensor based on piezoelectric ceramic resonator was developed for direct detection of cancer markers in the study. For the first time, a commercially available PZT ceramic resonator with high resonance frequency was utilized as transducer for a piezoelectric biosensor. A dual ceramic resonators scheme was designed wherein two ceramic resonators were connected in parallel: one resonator was used as the sensing unit and the other as the control unit. This arrangement minimizes environmental influences including temperature fluctuation, while achieving the required frequency stability for biosensing applications...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28299694/electrochemical-quartz-crystal-nanobalance-eqcn-based-biosensor-for-sensitive-detection-of-antibiotic-residues-in-milk
#18
Sunil Bhand, Geetesh K Mishra
An electrochemical quartz crystal nanobalance (EQCN), which provides real-time analysis of dynamic surface events, is a valuable tool for analyzing biomolecular interactions. EQCN biosensors are based on mass-sensitive measurements that can detect small mass changes caused by chemical binding to small piezoelectric crystals. Among the various biosensors, the piezoelectric biosensor is considered one of the most sensitive analytical techniques, capable of detecting antigens at picogram levels. EQCN is an effective monitoring technique for regulation of the antibiotics below the maximum residual limit (MRL)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28299680/application-of-a-nanostructured-enzymatic-biosensor-based-on-fullerene-and-gold-nanoparticles-to-polyphenol-detection
#19
Cristina Tortolini, Gabriella Sanzò, Riccarda Antiochia, Franco Mazzei, Gabriele Favero
Electrochemical biosensors provide an attractive means of analyzing the content of a biological sample due to the direct conversion of a biological event to an electronic signal. The signal transduction and the general performance of electrochemical biosensors are often determined by the surface architectures that connect the sensing element to the biological sample at the nanometer scale. The most common surface modification techniques, the various electrochemical transduction mechanisms, and the choice of the recognition receptor molecules all influence the ultimate sensitivity of the sensor...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28299678/an-electrochemical-dna-sensing-system-using-modified-nanoparticle-probes-for-detecting-methicillin-resistant-staphylococcus-aureus
#20
Hiroaki Sakamoto, Yoshihisa Amano, Takenori Satomura, Shin-Ichiro Suye
We have developed a novel, highly sensitive, biosensing system for detecting methicillin-resistant Staphylococcus aureus (MRSA). The system employs gold nanoparticles (AuNPs), magnetic nanoparticles (mNPs), and an electrochemical detection method. We have designed and synthesized ferrocene- and single-stranded DNA-conjugated nanoparticles that hybridize to MRSA DNA. Hybridized complexes are easily separated by taking advantage of mNPs. A current response could be obtained through the oxidation of ferrocene on the AuNP surface when a constant potential of +250 mV vs...
2017: Methods in Molecular Biology
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