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Apoptotic cells

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https://www.readbyqxmd.com/read/28102851/cellular-prion-protein-mediates-early-apoptotic-proteome-alternation-and-phospho-modification-in-human-neuroblastoma-cells
#1
Saima Zafar, Christina Behrens, Hassan Dihazi, Matthias Schmitz, Inga Zerr, Walter J Schulz-Schaeffer, Sanja Ramljak, Abdul R Asif
Anti-apoptotic properties of physiological and elevated levels of the cellular prion protein (PrP(c)) under stress conditions are well documented. Yet, detrimental effects of elevated PrP(c) levels under stress conditions, such as exposure to staurosporine (STS) have also been described. In the present study, we focused on discerning early apoptotic STS-induced proteome and phospho-proteome changes in SH-SY5Y human neuroblastoma cells stably transfected either with an empty or PRNP-containing vector, expressing physiological or supraphysiological levels of PrP(c), respectively...
January 19, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28102575/bcl-2-family-in-non-small-cell-lung-cancer-its-prognostic-and-therapeutic-implications
#2
Ping-Li Sun, Hironobu Sasano, Hongwen Gao
Non-small cell lung cancer (NSCLC) accounts for approximately 85% of all lung cancers. Despite significant advances in its research of tumor biology and therapy, the prognosis for this neoplasm has still remained poor. The great majority of anticancer agents, regardless of their mechanisms of action, exert their lethal actions on cancer cells by inducing apoptosis following drug-induced cellular damage. Many reported studies have evaluated the prognostic and therapeutic implications of apoptosis in lung cancer, but their exact clinical value has remained unclear...
January 19, 2017: Pathology International
https://www.readbyqxmd.com/read/28101579/role-of-trna-selenocysteine-1-associated-protein-1-in-the-proliferation-and-apoptosis-of-cardiomyocyte%C3%A2-like-h9c2-cells
#3
Meng-Di Li, Wan-Peng Cheng, Min-Xia Shi, Tang-Dong Ge, Xiao-Lin Zheng, Dong-Yuan Wu, Xiao-Yan Hu, Jin-Cheng Luo, Feng-Lan Li, Hui Li
Transfer RNA selenocysteine 1 associated protein 1 (Trnau1ap) serves an essential role in the synthesis of selenoproteins, which have critical functions in numerous biological processes. Selenium deficiency results in a variety of diseases, including cardiac disease. However, the mechanisms underlying myocardial injury induced by selenium deficiency remain unclear. The present study examined the effects of Trnau1ap under‑ and overexpression in cardiomyocyte‑like H9c2 cells, by transfection with small interfering RNA and an overexpression plasmid, respectively...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28101573/combining-doxorubicin-with-a-phenolic-extract-from-flaxseed-oil-evaluation-of-the-effect-on-two-breast-cancer-cell-lines
#4
Eliana Guerriero, Angela Sorice, Francesca Capone, Gabriella Storti, Giovanni Colonna, Gennaro Ciliberto, Susan Costantini
Breast cancer is one of the most frequently diagnosed forms of cancer and different treatments are used to block its progression. However, it still represents a very common cause of death in women. Doxorubicin (Dox) is reported as an effective agent in breast cancer treatment nonetheless it induces many side‑effects. For this reason, many laboratories are engaged in understanding how it is possible to decrease the drug concentration, considering that one of the possible solutions is to use drug synergy, combining it with natural substances...
February 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28101569/resveratrol-inhibits-adventitial-fibroblast-proliferation-and-induces-cell-apoptosis-through-the-sirt1-pathway
#5
Lin Ling, Shaohua Gu, Yan Cheng
Atherosclerosis is one of the most important causes of cardiovascular disease and studies have showed that adventitial fibroblasts, which are considered to be the most common cell type of the vascular adventitia, are involved in the development of early atherosclerotic plaques. Resveratrol is a plant polyphenolic compound confirmed to have anti‑atherosclerotic and cardioprotective effects. The aim of the present study was to investigate the effects of resveratrol on adventitial fibroblasts in vitro and to clarify the underlying mechanism...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28101417/investigation-of-terahertz-radiation-influence-on-rat-glial-cells
#6
Mariia Borovkova, Maria Serebriakova, Viacheslav Fedorov, Egor Sedykh, Vladimir Vaks, Alexander Lichutin, Alina Salnikova, Mikhail Khodzitsky
We studied an influence of continuous terahertz (THz) radiation (0.12 - 0.18 THz, average power density of 3.2 mW/cm(2)) on a rat glial cell line. A dose-dependent cytotoxic effect of THz radiation is demonstrated. After 1 minute of THz radiation exposure a relative number of apoptotic cells increased in 1.5 times, after 3 minutes it doubled. This result confirms the concept of biological hazard of intense THz radiation. Diagnostic applications of THz radiation can be restricted by the radiation power density and exposure time...
January 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28101374/arginine-methylation-of-usp9x-promotes-its-interaction-with-tdrd3-and-its-anti-apoptotic-activities-in-breast-cancer-cells
#7
Nithya Narayanan, Zhihao Wang, Ling Li, Yanzhong Yang
The Tudor domain-containing proteins are characterized by their specific interactions with methylated protein motifs, including methyl-arginines and methyl-lysines. The Tudor domain-containing protein 3 (TDRD3) is one of the major methyl-arginine effector molecules that recognizes methylated arginine residues on histones and the C-terminal domain of RNA polymerase II, and activates transcription. However, majority of the cellular TDRD3 localizes to the cytoplasm and its functions there are still elusive. Here, we have identified ubiquitin-specific protease 9 X-linked (USP9X) as a TDRD3-interacting protein by GST (glutathione S-transferase) pull-down and co-immunoprecipitation...
2017: Cell Discovery
https://www.readbyqxmd.com/read/28101228/increased-expression-of-pdia3-and-its-association-with-cancer-cell-proliferation-and-poor-prognosis-in-hepatocellular-carcinoma
#8
Hideyuki Takata, Mitsuhiro Kudo, Tetsushi Yamamoto, Junji Ueda, Kousuke Ishino, Wei-Xia Peng, Ryuichi Wada, Nobuhiko Taniai, Hiroshi Yoshida, Eiji Uchida, Zenya Naito
The prognosis of hepatocellular carcinoma (HCC) is unfavorable following complete tumor resection. The aim of the present study was to identify a molecule able to predict HCC prognosis through comprehensive protein profiling and to elucidate its clinicopathological significance. Comprehensive protein profiling of HCC was performed by liquid chromatography-tandem mass spectrometry. Through the bioinformatic analysis of proteins expressed differentially in HCC and non-HCC tissues, protein disulfide-isomerase A3 (PDIA3) was identified as a candidate for the prediction of prognosis...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28101183/protective-effect-of-berberine-against-oxidative-stress-induced-apoptosis-in-rat-bone-marrow-derived-mesenchymal-stem-cells
#9
Wangyang Li, Yamei Liu, Bin Wang, Yiwen Luo, Nianhong Hu, Dongfeng Chen, Xunchao Zhang, Yunpu Xiong
Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to be used for the treatment of delayed union, nonunion or persistent bone defects in MSC-based cell therapy. However, implantation of BMSCs into the fracture site is confronted with apoptosis on account of harsh conditions and oxidative stress. In the present study, the anti-apoptotic effects of berberine (BBR) on BMSCs subjected to hydrogen peroxide (H2O2) are investigated, and the potential underlying mechanisms are explored. Oxidative injury was induced by exposure to H2O2, and cell viability was assessed using a cell counting kit-8 assay...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28101164/paeoniflorin-attenuates-hippocampal-damage-in-a-rat-model-of-vascular-dementia
#10
Ying Zhang, Li-Li Wang, Yan Wu, Ning Wang, Shang-Ming Wang, Bin Zhang, Cui-Ge Shi, Shu-Cheng Zhang
Paeoniflorn (PF), the principal bioactive component of Paeoniae radix prescribed in traditional Chinese medicine, possesses a wide range of biological effects and exhibits neuroprotective effects in numerous diseases. Previous studies have demonstrated that PF significantly attenuates memory impairment in rats with vascular dementia (VD). In the present study, a bilateral common carotid artery occlusion (BCCAO) rat model was used to explore the underlying mechanisms of PF. The expression levels of neuron-specific enolase (NSE), S100β, B-cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein, cytochrome c and brain-derived neurotrophic factor (BDNF) in the hippocampus were measured by western blot analysis...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28101129/a-traditional-poly-herbal-medicine-le-pana-guliya-induces-apoptosis-in-hepg2-and-hela-cells-but-not-in-cc1-cells-an-in-vitro-assessment
#11
Nekadage Don Amal Wageesha, Preethi Soysa, Keerthi Atthanayake, Muhammad Iqbal Choudhary, Mahinda Ekanayake
"Le Pana Guliya" (LPG) is a polyherbal formulation which is used to treat different types of cancers in traditional medicine. In this study we describe in vitro efficacy and mechanism of action of LPG on two cancer cell lines (HepG2 and HeLa) compared with a normal cell line CC1. The MTT, LDH assays and protein synthesis were used to study antiproliferative activity of LPG while NO synthesis and GSH content were assayed to determine the oxidative stress exerted by LPG. Rhodamine 123 staining, caspase 3 activity, DNA fragmentation and microscopic examination of cells stained with ethidium bromide/acridine orange were used to identify the apoptosis mechanisms associated with LPG...
2017: Chemistry Central Journal
https://www.readbyqxmd.com/read/28100347/-salvianolate-protects-h9c2-cells-from-hypoxia-reoxygenation-injury-induced-apoptosis-by-attenuating-mitochondrial-dna-oxidative-damage
#12
R C Yue, X L Yang, R Y Zhang, S Liu, J Liu, J Zeng, H Liang, W Wang, H X Hu, C Y Zeng
Objective: To investigate the possible mechanism related to the protective effects of salvianolate in H9c2 cells underwent hypoxia/reoxygenation (H/R)-injury. Methods: H9c2 cells were divided into four groups: control group, salvianolate group (S group), H/R group, and salvianolate+ H/R group(S+ H/R group), in which the H9c2 cells were pretreated with salvianolate before H/R-treatment.Apoptotic cells were detected by Tunel assays and AnnexinⅤ-FITC apoptosis detection kit.The intracellular ATP level, the change of mitochondrial membrane potential and the mitochondrial DNA oxidative damage were also determined in these groups...
January 25, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28100275/cell-in-cell-structures-are-more-potent-predictors-of-outcome-than-senescence-or-apoptosis-in-head-and-neck-squamous-cell-carcinomas
#13
Hannah Schenker, Maike Büttner-Herold, Rainer Fietkau, Luitpold V Distel
BACKGROUND: This study sheds light on cell inactivating processes with focus on the phenomenon of cell-in-cell (CIC). Cell-in-cell describes a cell process where one cell is being engulfed by another non-professional phagocyte. We determined frequency and prognostic impact of CIC structures (CICs) as well as of senescent and apoptotic cells in head and neck squamous cell carcinomas (HNSCC). METHODS: These different forms of cell inactivation as well as the proportion of proliferating and tumor cells were assessed in 169 pre-radiochemotherapy biopsies and 32 post-therapy tumor resections by immunohistochemistry of tissue microarrays...
January 18, 2017: Radiation Oncology
https://www.readbyqxmd.com/read/28100259/antitumor-activity-of-mir-34a-in-peritoneal-mesothelioma-relies-on-c-met-and-axl-inhibition-persistent-activation-of-erk-and-akt-signaling-as-a-possible-cytoprotective-mechanism
#14
Rihan El Bezawy, Michelandrea De Cesare, Marzia Pennati, Marcello Deraco, Paolo Gandellini, Valentina Zuco, Nadia Zaffaroni
BACKGROUND: The value of microRNAs (miRNAs) as novel targets for cancer therapy is now widely recognized. However, no information is currently available on the expression/functional role of miRNAs in diffuse malignant peritoneal mesothelioma (DMPM), a rapidly lethal disease, poorly responsive to conventional treatments, for which the development of new therapeutic strategies is urgently needed. Here, we evaluated the expression and biological effects of miR-34a-one of the most widely deregulated miRNAs in cancer and for which a lipid-formulated mimic is already clinically available-in a large cohort of DMPM clinical samples and a unique collection of in house-developed preclinical models, with the aim to assess the potential of a miR-34a-based approach for disease treatment...
January 18, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28100091/the-effects-of-polyethylenimine-dna-nanoparticle-on-transcript-levels-of-apoptosis-related-genes
#15
Elham Pishavar, Mohammad Shafiei, Soghra Mehri, Mohammad Ramezani, Khalil Abnous
CONTEXT: Polyethylenimine (PEI) is a cationic polymer commonly used in gene transfer. Although numerous investigations have indicated that PEI can induce apoptosis/necrosis but the mechanism of its cytotoxicity is still poorly understood. OBJECTIVE: The purpose of this study was to investigate the effects of PEI/DNA complexes on the expression of apoptotic genes in human colon adenocarcinoma cells (HT29). METHODS: HT29 cells were exposed to PEI/DNA complex (C/P = 0...
January 18, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28100031/impact-of-melatonin-receptor-signaling-on-zeitgeber-time-dependent-changes-in-cell-proliferation-and-apoptosis-in-the-adult-murine-hippocampus
#16
Michaela Fredrich, Mareike Hampel, Kay Seidel, Elmar Christ, Horst-Werner Korf
The hippocampus is subjected to diurnal/circadian rhythms on both the morphological and molecular levels. Certain aspects of cell proliferation in the adult hippocampus are regulated by melatonin and accompanied by apoptosis to ensure proper tissue maintenance and function. The present study investigated Zeitgeber time (ZT)-dependent changes in cell proliferation and apoptosis in the adult murine hippocampus and their regulation by melatonin receptor type1 and type2 (MT1/2)-mediated signaling. Adult melatonin-proficient C3H/HeN mice and melatonin-proficient (C3H/HeN) mice with targeted deletion of MT1/2 were adapted to a 12-hours light, 12-hours dark photoperiod and were sacrificed at ZT00, ZT06, ZT12, and ZT18...
January 18, 2017: Hippocampus
https://www.readbyqxmd.com/read/28099966/involvement-of-xbp1s-in-blue-light-induced-a2e-containing-retinal-pigment-epithelium-cell-death
#17
Bing Lu, Pengfei Zhang, Minwen Zhou, Wenqiu Wang, Qing Gu, Jingyang Feng, Xueting Luo, Xiangjun Sun, Fenghua Wang, Xiaodong Sun
PURPOSE: Retinal pigment epithelium (RPE) cell dysfunction is essential to the development of retinal degenerative disease. This study was designed to investigate how spliced X-box-binding protein 1 (XBP1s) regulates different modes of RPE cell death in vitro. METHODS: Human ARPE19 cells were incubated with 25 μM N-retinylidene-N-retinylethanolamine (A2E) and irradiated with blue light. Expressions of glucose-regulated protein 78 (GRP78) and XBP1s were detected by real-time quantitative PCR and Western blot...
January 19, 2017: Ophthalmic Research
https://www.readbyqxmd.com/read/28099931/a-new-semisynthetic-cardenolide-analog-3%C3%AE-2-1-amantadine-1-on-ethylamine-digitoxigenin-amantadig-affects-g2-m-cell-cycle-arrest-and-mirna-expression-profiles-and-enhances-proapoptotic-survivin-2b-expression-in-renal-cell-carcinoma-cell-lines
#18
Elke Nolte, Sven Wach, Izabella Thais Silva, Sabine Lukat, Arif B Ekici, Jennifer Munkert, Frieder Müller-Uri, Wolfgang Kreis, Cláudia Maria Oliveira Simões, Julio Vera, Bernd Wullich, Helge Taubert, Xin Lai
Cardiac glycosides are well known in the treatment of cardiovascular diseases; however, their application as treatment option for cancer patients is under discussion. We showed that the cardiac glycoside digitoxin and its analog AMANTADIG can inhibit the growth of renal cell carcinoma (RCC) cell lines and increase G2/M cell cycle arrest. To identify the signaling pathways and molecular basis of this G2/M arrest, microRNAs were profiled using microRNA arrays. Cardiac glycoside treatment significantly deregulated two microRNAs, miR-2278 and miR-670-5p...
January 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28099869/amitriptyline-down-regulates-coenzyme-q10-biosynthesis-in-lung-cancer-cells
#19
Tamara Ortiz, Marina Villanueva-Paz, Eduardo Díaz-Parrado, Matilde Illanes, Ana Fernández-Rodríguez, José A Sánchez-Alcázar, Manuel de Miguel
Amitriptyline, a tricyclic antidepressant, has been proposed as an antitumoral drug in oxidative therapy. Its pro-apoptotic effects, mediated by high reactive oxygen species generation, have been already described. In this study we analysed the effect of amitriptyline on the biosynthesis of coenzyme Q10 (CoQ), an essential component for electron transport and a potent membrane antioxidant involved in redox signaling. We treated H460 cells, a non-small-cell lung cancer cell line, with amitriptyline and we analyzed CoQ levels by HPLC and CoQ biosynthesis rate, as well as the enzymes involved in CoQ biosynthesis by real-time PCR and Western blot...
January 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28099785/a-dual-topoisomerase-inhibitor-of-intense-pro-apoptotic-and-antileukemic-nature-for-cancer-treatment
#20
Christopher Meier, Tamara N Steinhauer, Fabian Koczian, Birte Plitzko, Katharina Jarolim, Ulrich Girreser, Simone Braig, Doris Marko, Angelika M Vollmar, Bernd Clement
Classic cytotoxic drugs constantly remain an indispensable instrument in antitumor therapy due to their effectiveness and a more prevalent insensibility against tumor resistance mechanisms. We describe the favorable properties of P8-D6, a powerful inductor of apoptosis caused by an equipotent inhibition of human topoisomerase I and II activities. A broad spectrum effect against human tumor cell lines in nanomolar concentrations as well as strong anti-leukemic effects were shown and proven to be superior to marketed topo-targeting drugs and dual topoisomerase inhibitors in clinical trials...
January 18, 2017: ChemMedChem
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