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https://www.readbyqxmd.com/read/28924139/srphi-ratiometric-ph-biosensors-for-super-resolution-microscopy
#1
Douglas S Richardson, Carola Gregor, Franziska R Winter, Nicolai T Urban, Steffen J Sahl, Katrin I Willig, Stefan W Hell
Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy...
September 18, 2017: Nature Communications
https://www.readbyqxmd.com/read/28923121/-i-think-it-is-right-a-qualitative-exploration-of-the-acceptability-and-desired-future-use-of-oral-swab-and-finger-prick-hiv-self-tests-by-lay-users-in-kwazulu-natal-south-africa
#2
Lucia Knight, Tawanda Makusha, Jeanette Lim, Roger Peck, Miriam Taegtmeyer, Heidi van Rooyen
BACKGROUND: The uptake of HIV testing has increased in sub-Saharan Africa over the past three decades. However, the proportion of people aware of their HIV status remains lower than required to change the pandemic. HIV self-testing (HIVST) may meet this gap. Assessment of readiness for and the acceptability of HIVST by lay users in South Africa is limited. This paper presents results from a formative study designed to assess the perceived usability and acceptability of HIVST among lay users using several self-test prototypes...
September 18, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28923069/perceived-barriers-and-enablers-to-participation-in-a-community-tailored-physical-activity-program-with-indigenous-australians-in-a-regional-and-rural-setting-a-qualitative-study
#3
Ashleigh Sushames, Terry Engelberg, Klaus Gebel
BACKGROUND: Aboriginal and Torres Strait Islander people have higher rates of chronic disease and a lower life expectancy than non-Indigenous Australians. In non-urban areas these health disparities are even larger. The aim of this qualitative study was to explore perceived barriers and enablers to attending an eight-week physical activity program in a rural and regional setting which aimed to improve health outcomes, but had a low attendance rate. METHODS: Thirty-four Indigenous Australians participated in the intervention from the rural (n = 12) and the regional (n = 22) community...
September 18, 2017: International Journal for Equity in Health
https://www.readbyqxmd.com/read/28917222/validation-of-summer-and-winter-elisa-measurements-of-serum-25-hydroxyvitamin-d-concentrations-in-mongolia
#4
Sabri Bromage, Daria Tselmen, Gary Bradwin, Michael F Holick, Davaasaambuu Ganmaa
BACKGROUND AND OBJECTIVES: Assay cost, quality, and availability pose challenges for vitamin D surveys in limited resource settings. This study aimed to validate an inexpensive vitamin D assay (ELISA) under real-world conditions in Mongolia, the northernmost developing country, to characterize the assay's usefulness and inform the design of epidemiologic studies in similar regions. METHODS AND STUDY DESIGN: We collected paired summer and winter serum samples from 120 men and women (aged 20-57 years) in urban and rural Mongolia, analyzed each sample for 25(OH)D concentration using both Immunodiagnostic Systems ELISA and DiaSorin LIAISON 25(OH)D TOTAL, and compared the assays using multiple statistics...
2017: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28905095/growing-canopy-on-a-college-campus-understanding-urban-forest-change-through-archival-records-and-aerial-photography
#5
Lara A Roman, Jason P Fristensky, Theodore S Eisenman, Eric J Greenfield, Robert E Lundgren, Chloe E Cerwinka, David A Hewitt, Caitlin C Welsh
Many municipalities are setting ambitious tree canopy cover goals to increase the extent of their urban forests. A historical perspective on urban forest development can help cities strategize how to establish and achieve appropriate tree cover targets. To understand how long-term urban forest change occurs, we examined the history of trees on an urban college campus: the University of Pennsylvania in Philadelphia, PA. Using a mixed methods approach, including qualitative assessments of archival records (1870-2017), complemented by quantitative analysis of tree cover from aerial imagery (1970-2012), our analysis revealed drastic canopy cover increase in the late 20th and early 21st centuries along with the principle mechanisms of that change...
September 13, 2017: Environmental Management
https://www.readbyqxmd.com/read/28904814/factors-impacting-perceived-safety-among-staff-working-on-mental-health-wards
#6
Alina Haines, Andrew Brown, Rhiannah McCabe, Michelle Rogerson, Richard Whittington
BACKGROUND: Safety at work is a core issue for mental health staff working on in-patient units. At present, there is a limited theoretical base regarding which factors may affect staff perceptions of safety. AIMS: This study attempted to identify which factors affect perceived staff safety working on in-patient mental health wards. METHOD: A cross-sectional design was employed across 101 forensic and non-forensic mental health wards, over seven National Health Service trusts nationally...
September 2017: BJPsych Open
https://www.readbyqxmd.com/read/28903935/glaucoma-and-intraocular-pressure-in-epic-norfolk-eye-study-cross-sectional-study
#7
Michelle P Y Chan, David C Broadway, Anthony P Khawaja, Jennifer L Y Yip, David F Garway-Heath, Jennifer M Burr, Robert Luben, Shabina Hayat, Nichola Dalzell, Kay-Tee Khaw, Paul J Foster
Objectives To report the distribution of intraocular pressure (IOP) by age and sex and the prevalence of glaucoma.Design Community based cross sectional observational study.Setting EPIC-Norfolk cohort in Norwich and the surrounding rural and urban areas.Participants 8623 participants aged 48-92 recruited from the community who underwent ocular examination to identify glaucoma.Main outcome measures Prevalence and characteristics of glaucoma, distribution of IOP, and the sensitivity and specificity of IOP for case finding for glaucoma...
September 13, 2017: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/28902317/-methodological-design-of-the-national-health-and-nutrition-survey-2016
#8
Martín Romero-Martínez, Teresa Shamah-Levy, Lucía Cuevas-Nasu, Ignacio Méndez Gómez-Humarán, Elsa Berenice Gaona-Pineda, Luz María Gómez-Acosta, Juan Ángel Rivera-Dommarco, Mauricio Hernández-Ávila
Objective: Describe the design methodology of the halfway health and nutrition national survey (Ensanut-MC) 2016. Materials and methods: The Ensanut-MC is a national probabilistic survey whose objective population are the inhabitants of private households in Mexico. The sample size was determined to make inferences on the urban and rural areas in four regions. Describes main design elements: target population, topics of study, sampling procedure, measurement procedure and logistics organization...
May 2017: Salud Pública de México
https://www.readbyqxmd.com/read/28900893/associations-of-neighborhood-environmental-attributes-with-walking-in-japan-moderating-effects-of-area-level-socioeconomic-status
#9
Mohammad Javad Koohsari, Tomoya Hanibuchi, Tomoki Nakaya, Ai Shibata, Kaori Ishii, Yung Liao, Koichiro Oka, Takemi Sugiyama
Several studies have examined how the associations of built environment attributes with walking behaviors may be moderated by socioeconomic status (SES). Such understanding is important to address socioeconomic inequalities in health through urban design initiatives. However, to date, there is no study examining the moderation effects of SES in the relationships of environmental attributes and walking in non-Western countries. The current study aims to examine associations of environmental attributes with walking behaviors among Japanese adults, and to test whether these associations were moderated by area-level SES...
September 12, 2017: Journal of Urban Health: Bulletin of the New York Academy of Medicine
https://www.readbyqxmd.com/read/28900788/take-care-of-your-neighborhood
#10
Elmer E Huerta, Patricia Weeks-Coulthurst, Courtney Williams, Sandra M Swain
PURPOSE: Urban women in certain Washington, DC neighborhoods present with advanced breast cancer at high rates despite access to health insurance and health care. METHODS: Through a two-phase intervention, community health workers (CHWs) educated and surveyed individuals regarding healthcare utilization and breast health and cancer awareness. In phase I, CHWs educated and administered a survey to 1092 women, of whom 95.1% had health insurance, in an attempt to explain the high rate of advanced breast cancer despite having health insurance...
September 12, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28899469/-association-between-family-environment-and-developmental-coordination-disorder-in-preschool-children
#11
Li-Fei Liu, Lan Lu, Hong-Ni Yue, Bei Huan, Gui-Xiong Gu, Hua Jin, Yu-Mei Wang
OBJECTIVE: To investigate the influence of family environment on developmental coordination disorder (DCD) in preschool children. METHODS: Stratified random cluster sampling was used to select 1 727 children (4-6 years old). The Movement Assessment Battery for Children was used to screen out the children with DCD. The Family Environment Scale on Motor Development for Preschool Urban Children and a self-designed questionnaire were used to assess family environment...
September 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28899406/forecasting-the-regional-distribution-and-sufficiency-of-physicians-in-japan-with-a-coupled-system-dynamics-geographic-information-system-model
#12
Tomoki Ishikawa, Kensuke Fujiwara, Hisateru Ohba, Teppei Suzuki, Katsuhiko Ogasawara
BACKGROUND: In Japan, the shortage of physicians has been recognized as a major medical issue. In our previous study, we reported that the absolute shortage will be resolved in the long term, but maldistribution among specialties will persist. To address regional shortage, several Japanese medical schools increased existing quota and established "regional quotas." This study aims to assist policy makers in designing effective policies; we built a model for forecasting physician numbers by region to evaluate future physician supply-demand balances...
September 12, 2017: Human Resources for Health
https://www.readbyqxmd.com/read/28898938/characterization-of-wash-off-from-urban-impervious-surfaces-and-suds-design-criteria-for-source-control-under-semi-arid-conditions
#13
Ignacio Andrés-Doménech, Carmen Hernández-Crespo, Miguel Martín, Valerio C Andrés-Valeri
Knowledge about pollutant wash-off from urban impervious surfaces is a key feature for developing effective management strategies. Accordingly, further information is required about urban areas under semi-arid climate conditions at the sub-catchment scale. This is important for designing source control systems for pollution. In this study, a characterization of pollutant wash-off has been performed over sixteen months, at the sub-catchment scale for urban roads as impervious surfaces. The study was conducted in Valencia, Spain, a city with a Mediterranean climate...
September 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28895027/the-aging-urban-brain-analyzing-outdoor-physical-activity-using-the-emotiv-affectiv-suite-in-older-people
#14
Chris Neale, Peter Aspinall, Jenny Roe, Sara Tilley, Panagiotis Mavros, Steve Cinderby, Richard Coyne, Neil Thin, Gary Bennett, Catharine Ward Thompson
This research directly assesses older people's neural activation in response to a changing urban environment while walking, as measured by electroencephalography (EEG). The study builds on previous research that shows changes in cortical activity while moving through different urban settings. The current study extends this methodology to explore previously unstudied outcomes in older people aged 65 years or more (n = 95). Participants were recruited to walk one of six scenarios pairing urban busy (a commercial street with traffic), urban quiet (a residential street) and urban green (a public park) spaces in a counterbalanced design, wearing a mobile Emotiv EEG headset to record real-time neural responses to place...
September 11, 2017: Journal of Urban Health: Bulletin of the New York Academy of Medicine
https://www.readbyqxmd.com/read/28894532/an-ethno-pharmacological-study-of-plants-used-for-traditional-medication-in-tangail-district-bangladesh
#15
A H M Mahmudur Rahman, Mahmoud Rafieian-Kopaei
BACKGROUND: In Bangladesh, folk medicinal practitioners are called "Kaviraj" and are consulted for treatment of various ailments by a large part of the rural and urban population. There are some previous studies conducted in the Tangail district of Bangladesh about medicinal plants, but there is no relevant information about this aspect in some parts of this district. AIM: To conduct an ethno-pharmacological survey among the "Kaviraj" of two upazilas (regions) in Tangail district, namely Tangail Sadar Upazila and Nagarpur Upazila, to identify the trouble-free formulations of medicinal plants for various diseases used by the folk medicine practitioners on or after other forms of medical practices...
July 2017: Electronic Physician
https://www.readbyqxmd.com/read/28893178/evaluation-design-of-urban-health-centres-europe-uhce-preventive-integrated-health-and-social-care-for-community-dwelling-older-persons-in-five-european-cities
#16
Carmen B Franse, Antonius J J Voorham, Rob van Staveren, Elin Koppelaar, Rens Martijn, Elisa Valía-Cotanda, Tamara Alhambra-Borrás, Tasos Rentoumis, Lovorka Bilajac, Vanja Vasiljev Marchesi, Tomislav Rukavina, Arpana Verma, Greg Williams, Gary Clough, Jorge Garcés-Ferrer, Francesco Mattace Raso, Hein Raat
BACKGROUND: Older persons often have interacting physical and social problems and complex care needs. An integrated care approach in the local context with collaborations between community-, social-, and health-focused organisations can contribute to the promotion of independent living and quality of life. In the Urban Health Centres Europe (UHCE) project, five European cities (Greater Manchester, United Kingdom; Pallini (in Greater Athens Area), Greece; Rijeka, Croatia; Rotterdam, the Netherlands; and Valencia, Spain) develop and implement a care template that integrates health and social care and includes a preventive approach...
September 11, 2017: BMC Geriatrics
https://www.readbyqxmd.com/read/28891541/inequity-in-access-to-childhood-immunization-in-enugu-urban-southeast-nigeria
#17
B S Uzochukwu, C C Okeke, E Envuladu, C Mbachu, C Okwuosa, O E Onwujekwe
BACKGROUND: The Nigerian National Programme on Immunization aims at increasing the immunization coverage of children under 1 year of age. However, there is still a gap between the national immunization targets and the immunization coverage rates, and data are rarely disaggregated according to socioeconomic status. As a result, there is a dearth of information about the coverage of subgroups, especially at the local level. This study determined the socioeconomic differentials in immunization coverage for children under 5 years and under 1 year in Enugu urban, Southeast Nigeria...
August 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28891407/substance-use-and-self-harm-case-studies-from-patients-admitted-to-an-urban-hospital-following-medically-serious-self-harm
#18
Elsie Breet, Jason Bantjes
Few qualitative studies have explored the relationship between substance use and self-harm. We employed a multiple-case study research design to analyze data from 80 patients who were admitted to a hospital in South Africa following self-harm. Our analysis revealed, from the perspective of patients, a number of distinct ways in which substance use is implicated in self-harm. Some patients reported that substance intoxication resulted in poor decision making and impulsivity, which led to self-harm. Others said substance use facilitated their self-harm...
September 1, 2017: Qualitative Health Research
https://www.readbyqxmd.com/read/28890948/green-environment-and-incident-depression-in-south-africa-a-geospatial-analysis-and-mental-health-implications-in-a-resource-limited-setting
#19
Andrew Tomita, Alain M Vandormael, Diego Cuadros, Enrico Di Minin, Vuokko Heikinheimo, Frank Tanser, Rob Slotow, Jonathan K Burns
BACKGROUND: Unprecedented levels of habitat transformation and rapid urbanisation are changing the way individuals interrelate with the natural environment in developing countries with high economic disparities. Although the potential benefit of green environments for mental health has been recognised, population-level evidence to this effect is scarce. We investigated the effect of green living environment in potentially countering incident depression in a nationally representative survey in South Africa...
July 2017: The Lancet. Planetary health
https://www.readbyqxmd.com/read/28890794/engaging-experts-and-patients-to-refine-the-nutrition-literacy-assessment-instrument
#20
Heather D Gibbs, Susan Harvey, Sarah Owens, Diane Boyle, Debra K Sullivan
BACKGROUND: An objective measure of nutrition literacy is unavailable for use in the primary care population. The Nutrition Literacy Assessment instrument (NLit) is a tool designed to measure nutrition literacy across six domains and has been previously piloted in breast cancer and parent populations. The purpose of this research was to engage nutrition experts and patients to guide revisions of the NLit for use in adult primary care. METHODS: Experts (n=5) reviewed each item in the NLit using a survey to assign rankings of their agreement according to relevance, clarity, and reading difficulty...
2017: BMC Nutrition
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