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Kasper Jacobsen Kyng, Anders Valdemar Edhager, Tine Brink Henriksen, Christer Zøylner Swan, Niels Gregersen, Johan Palmfeldt
BACKGROUND: Biomarkers may qualify diagnosis, treatment allocation, and prognostication in neonatal encephalopathy. Biomarker development is challenged by competing etiologies, inter-individual genetic variability, and a lack of specific neonatal markers. To address these challenges, we used a standardized neonatal hypoxic-ischemic (HI) encephalopathy model with pre- and post-HI sampling of cerebrospinal fluid (CSF) and plasma. OBJECTIVES: The study aimed to identify novel candidate protein biomarkers of HI encephalopathy in a newborn piglet model in CSF and plasma...
July 19, 2018: Neonatology
Zhong-Ling Qiu, Wei-Jun Wei, Zhen-Kui Sun, Chen-Tian Shen, Hong-Jun Song, Xin-Yun Zhang, Guo-Qiang Zhang, Xiao-Yue Chen, Quan-Yong Luo
BACKGROUND/AIMS: As biomarkers, circulating tumor cells (CTCs) from solid tumors can predict metastases and prognoses, and help monitor treatment efficacy. However, conventional CellSearch methods have low sensitivity to differentiated thyroid cancer (DTC) CTCs. In this study, for the first time, we used negative enriching (NE) immunofluorescence-in situ hybridization (iFISH) of chromosome 8 to capture and identify CTCs in DTC patients; and investigated how CTCs correlate with clinicopathological factors and prognosis in DTC patients with distant metastases (DM)...
July 19, 2018: Cellular Physiology and Biochemistry
Yi-Xin Wang, Heng-Gui Chen, Xian-Dong Li, Ying-Jun Chen, Chong Liu, Wei Feng, Qiang Zeng, Peng Wang, An Pan, Wen-Qing Lu
Widespread human exposure to vanadium has been well documented. Vanadium exposure was reported to induce male reproductive toxicity in toxicological studies, yet human epidemiologic studies are lacking. Here we determined the associations between environmental exposure to vanadium and semen quality, spermatozoa DNA damage and serum reproductive hormones. Concentrations of vanadium in seminal plasma and repeated urine samples were determined among 764 men recruited from a reproductive medicine centre. Associations of vanadium concentrations with semen quality parameters (n = 764), DNA integrity measures (n = 404) and serum reproductive hormones (n = 381) were assessed by logistic or linear regression models with adjustment for potential confounders...
July 16, 2018: Science of the Total Environment
Sean T Garrity, David Sarraf, K Bailey Freund, Srinivas R Sadda
Nonneovascular (dry) AMD is a retinal disease with potential for significant central visual impairment. The hallmarks of this disease are macular drusen, RPE alterations, and geographic atrophy (GA). Classification schemes for nonneovascular AMD have evolved over the years as major advances in retinal imaging have enabled a greater understanding of disease pathophysiology. The original classifications of nonneovascular AMD were based on color fundus photography (CFP), while more modern schemes rely on a multimodal imaging approach...
March 20, 2018: Investigative Ophthalmology & Visual Science
Christian Schori, Christian Trachsel, Jonas Grossmann, Ioanna Zygoula, Daniel Barthelmes, Christian Grimm
Purpose: In contrast to neovascular AMD (nAMD), no treatment option exists for dry AMD. Hence, the identification of specific biomarkers is required to facilitate diagnosis and therapy of dry AMD. Methods: The proteome of 34 vitreous humor samples (dry AMD: n = 6; nAMD: n = 10; proliferative diabetic retinopathy [PDR]: n = 9; epiretinal membrane [ERM]: n = 9) was analyzed by liquid chromatography coupled mass spectrometry. Then, label-free relative quantification of dry AMD, nAMD, and PDR relative to ERM, which was defined as the reference group, was performed...
March 20, 2018: Investigative Ophthalmology & Visual Science
Cirous Dehghani, Shaun Frost, Rajiv Jayasena, Colin L Masters, Yogesan Kanagasingam
Globally, Alzheimer's disease (AD) is a growing health and economic challenge that has no effective cure. Recent clinical trials indicate that preclinical treatment may be required but a routine screening tool for AD has been elusive. Hence, a simple, yet sensitive biomarker for preclinical AD, when the disease is most likely to be amenable to treatment, is lacking. Due to several features, the eye has been explored for this purpose and, among the ocular tissues, the retina has received the most attention. Currently, major works investigating the potential AD diagnosis by detecting amyloid-β (Aβ) signatures in the retinal tissue are underway, while the anterior eye is more accessible for in vivo imaging and examination...
July 2, 2018: Investigative Ophthalmology & Visual Science
Nadine Gerber-Hollbach, Kim Plattner, Olivia E O'Leary, Paul Jenoe, Suzette Moes, Beatrice Drexler, Andreas Schoetzau, Jörg P Halter, David Goldblum
Purpose: To date, no biomarkers for ocular graft versus host disease (GvHD), a frequent complication following allogeneic hematopoietic cell transplantation (HCT), exist. In this prospective study, we evaluated the potential of human tear proteins as biomarkers for ocular GvHD. Methods: Tears from 10 patients with moderate-to-severe ocular GvHD were compared to 10 patients without ocular GvHD. After a full ocular surface clinical examination, tears were collected onto Schirmer strips and protein composition was analyzed by liquid chromatography tandem mass spectrometry...
July 2, 2018: Investigative Ophthalmology & Visual Science
Helle Glud Binderup, Jonna Skov Madsen, Niels Henrik Helweg Heegaard, Kim Houlind, Rikke Fredslund Andersen, Claus Lohman Brasen
Numerous studies have reported a potential role for circulating microRNAs as biomarkers in a wide variety of diseases. However, there is a critical reproducibility challenge some of which might be due to differences in preanalytical and/or analytical factors. Thus, in the current study we systematically investigated the impact of selected preanalytical and analytical variables on the measured microRNA levels in plasma. Similar levels of microRNA were found in platelet-poor plasma obtained by dual compared to prolonged single centrifugation...
2018: PloS One
Afsaneh Mojtabanezhad Shariatpanahi, Maryam Yassi, Mehdi Nouraie, Amirhossein Sahebkar, Fatemeh Varshoee Tabrizi, Mohammad Amin Kerachian
A large number of tumor-related methylated genes have been suggested to be of diagnostic and prognostic values for CRC when analyzed in patients' stool samples; however, reported sensitivities and specificities have been inconsistent and widely varied. This meta-analysis was conducted to assess the detection accuracy of stool DNA methylation assay in CRC, early stages of CRC (advanced adenoma, non-advanced adenomas) and hyperplastic polyps, separately. We searched MEDLINE, Web of Science, Scopus and Google Scholar databases until May 1, 2016...
2018: PloS One
Lorena Fontán, Qi Qiao, John M Hatcher, Gabriella Casalena, Ilkay Us, Matt Teater, Matthew Durant, Guangyan Du, Min Xia, Natalia Bilchuk, Spandan Chennamadhavuni, Giuseppe Palladino, Giorgio Inghirami, Ulrike Philippar, Hao Wu, David A Scott, Nathanael S Gray, Ari Melnick
The MALT1 paracaspase plays an essential role in Activated B-cell like Diffuse Large B cell Lymphoma (ABC DLBCL) downstream of B cell and Toll-like receptor pathway genes mutated in these tumors. Although MALT1 is considered to be a compelling therapeutic target, development of tractable and specific MALT1 protease inhibitors has thus far been elusive. Herein, we developed a target engagement assay that provides a quantitative readout for specific MALT1 inhibitory effects in living cells. This enabled a structure-guided medicinal chemistry effort culminating in the discovery of pharmacologically tractable irreversible substrate-mimetic compounds that bind the MALT1 active site...
July 19, 2018: Journal of Clinical Investigation
Radwa Sharaf, Guinevere Q Lee, Xiaoming Sun, Behzad Etemad, Layla M Aboukhater, Zixin Hu, Zabrina L Brumme, Evgenia Aga, Ronald J Bosch, Ying Wen, Golnaz Namazi, Ce Gao, Edward P Acosta, Rajesh T Gandhi, Jeffrey M Jacobson, Daniel Skiest, David M Margolis, Ronald Mitsuyasu, Paul Volberding, Elizabeth Connick, Daniel R Kuritzkes, Michael M Lederman, Xu G Yu, Mathias Lichterfeld, Jonathan Z Li
HIV post-treatment controllers (PTCs) represent a natural model of sustained HIV remission, but they are rare and little is known about their viral reservoir. We obtained 1450 proviral sequences after near-full-length amplification for 10 PTCs and 16 post-treatment non-controllers (NCs). Before treatment interruption, the median intact and total reservoir size in PTCs was 7-fold lower than in NCs, but the proportion of intact, defective and total clonally-expanded viral genomes was not significantly different between the two groups...
July 19, 2018: Journal of Clinical Investigation
Maria Salinas, Maite López-Garrigós, Emilio Flores, Javier Lugo, Carlos Leiva-Salinas
Background The clinical laboratory plays a crucial role in the diagnosis and monitoring of chronic kidney disease. The quantitative measurement of urine albumin in a spot sample, expressed as ratio per creatinine (ACR) is the most frequently used biomarker for such a purpose. Our aim was to evaluate the diagnostic performances of a strip for measuring ACR for differentiating patients who are candidates for subsequent albumin quantification, and to evaluate the economic effects of its implementation. Methods We systematically measured strip analysis when quantitative urinary albumin was requested...
July 19, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Rosa De Las Heras, José Luis Rodríguez-Gil, Jon San Sebastián Sauto, Paz Sánchez Sánchez, Myriam Catalá
The development of field-based biomarkers can allow for a more reliable assessment of the exposure of organisms to pollutants. Different sampling sites, along two streams running through an irrigable agricultural area, were selected to evaluate the effect of agrochemical load on the measured endpoints. The levels of lipid peroxidation were evaluated in several organs of Procambarus clarkii. The same method was applied to leaves of two woody species. Determining levels of MDA (malonaldehyde) by thiobarbituric acid reactive substances assay allows measuring the levels of lipid peroxidation...
July 19, 2018: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Lv Yong, Bai Guang, Li Yan
BACKGROUND: The rarity of acute liver failure, along with its severity and heterogeneity, has resulted in a very limited evidence to understand of the molecular mechanism. To analyze the differentially expressed genes (DEGs) in the Hepatitis B Virus -Associated Acute Liver Failure and elucidate the biological significance of the DEGs. METHODS: Firstly, differentially expressed genes (DEGs) between seventeen HBV-associated acute liver failure liver samples and ten control normal liver samples were identified by R package...
April 2018: Acta Gastro-enterologica Belgica
Youssra Haouami, Tarak Dhaouadi, Imen Sfar, Mongi Bacha, Tahar Gargah, Rafika Bardi, Ezzeddine Abderrahim, Rym Goucha, Taïeb Ben Abdallah, Yousr Gorgi
Th17 cell subset has been implicated in autoimmune diseases, tumor immunity and, transplant rejection. In order to investigate the role of IL-17/IL-23 pathway in allograft outcome, intragraft expression of IL-17 mRNA and single nucleotide polymorphisms (SNPs) of IL-17A, IL-17F, IL-17RC, and IL23R genes were evaluated with a quantification of IL-17A, IL-17F, and IL-23 plasma levels. This study revealed that recipients with acute rejection (AR) had a significant increase in IL-17A mRNA expression levels after transplantation compared to controls (P = 0...
July 19, 2018: Journal of Leukocyte Biology
L-L Bi, F Han, X-M Zhang, Y-Y Li
OBJECTIVE: The aim of this study was to investigate the roles of MT1JP and β-catenin in retinoblastoma. PATIENTS AND METHODS: We performed quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) to quantify the expressions of MT1JP and β-catenin in 44 retinoblastoma tissues and matched non-tumor tissues. What's more, retinoblastoma cell lines were transfected with pcDNA3.1-MT1JP, after which proliferation, cell cycle, apoptosis, and expression of β-catenin as well as its downstream targets were assayed...
July 2018: European Review for Medical and Pharmacological Sciences
H-F Li, Q Xie, Q-W Nie, X Ye
OBJECTIVE: To determine whether prostate-specific antigen (PSA) could serve as a biomarker for breast cancer. MATERIALS AND METHODS: We performed an electronic search on Medline, PubMed, SPRINGER, John Wiley, Science Direct, EBSCO, CNKI and Wanfang Data to identify relevant studies for our meta-analysis. The search terms included ['prostate specific antigen' or 'PSA' (MESH)] and ['breast cancer' or 'breast carcinoma' (MESH)]. RESULTS: A comprehensive meta-analysis of 10 studies comprising of 770 cases and 799 controls were included...
July 2018: European Review for Medical and Pharmacological Sciences
Y Yuan, X-Y Xu, H-G Zheng, B-J Hua
OBJECTIVE: Increasing studies have investigated the prognostic value of high miR-21 expression in non-small cell lung cancer (NSCLC) with inconsistent results. We conducted this meta-analysis to explore whether the expression of miR-21 was associated with prognosis in NSCLC patients. MATERIALS AND METHODS: We systematically searched Medline, EMBASE, Web of Science and Cochrane Library for relevant studies. Studies exploring the relationship between miR-21 expression and NSCLC prognosis and clinical pathology, and reporting enough data to get the hazard ratio (HR) and 95% confidence intervals (CIs), were included...
July 2018: European Review for Medical and Pharmacological Sciences
S-W Li, K-Z Hu, S-C Chen, S-L Liu, Y-H Wang
OBJECTIVE: Expression of long non-coding RNA LOC730101 (LOC730101) has been closely linked to the carcinogenesis of osteosarcoma and has been shown to function as a tumor promoter. However, the clinical significance of LOC730101 remains unknown. Our present study aimed to investigate the feasibility of LOC730101 as a novel prognostic biomarker for osteosarcoma. PATIENTS AND METHODS: Quantitative Real-time RT-PCR (qRT-PCR) was performed to examine LOC730101 expression in osteosarcoma tissues and adjacent non-cancerous tissues...
July 2018: European Review for Medical and Pharmacological Sciences
Lian-Hua Fu, Chao Qi, Jing Lin, Peng Huang
Glucose oxidase (GOx) is an endogenous oxido-reductase that is widely distributed in living organisms. Over recent years, GOx has attracted increasing interest in the biomedical field due to its inherent biocompatibility, non-toxicity, and unique catalysis against β-d-glucose. GOx efficiently catalyzes the oxidization of glucose into gluconic acid and hydrogen peroxide (H2O2), which can be employed by various biosensors for the detection of cancer biomarkers. Various cancer therapeutic strategies have also been developed based on the catalytic chemistry of GOx: (1) the consumption of glucose provides an alternative strategy for cancer-starvation therapy; (2) the consumption of oxygen increases tumor hypoxia, which can be harnessed for hypoxia-activated therapy; (3) the generation of gluconic acid enhances the acidity of the tumor microenvironment, which can trigger pH-responsive drug release; (4) the generation of H2O2 increases the levels of tumor oxidative stress, and the H2O2 can be converted into toxic hydroxyl radicals that can kill cancer cells upon exposure to light irradiation or via the Fenton reaction...
July 19, 2018: Chemical Society Reviews
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