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Complex 1

Simone Angeloni, Luciano Navarini, Gianni Sagratini, Elisabetta Torregiani, Sauro Vittori, Giovanni Caprioli
Lignans are polyphenolic compounds that are considered phytoestrogens for their plant origins and they possess different biological activities. Three different extraction methods, i.e., 'dilute and shoot', acidic hydrolysis and enzymatic digestion, have been compared for extracting lignans (secoisolariciresinol, SECO, matairesinol, MAT and lariciresinol LARI) from espresso coffee (EC) by using high performance liquid chromatography (HPLC) tandem mass spectrometry (MS/MS). The best recovery values (SECO: 97%, LARI: 98% and MAT: 93%) were obtained by using enzymatic hydrolysis with Clara-Diastase at 10% (w/v), keeping the sample at 37 °C for 3 hours...
June 20, 2018: Journal of Mass Spectrometry: JMS
Tripti Mandal, Anowar Hossain, Anamika Dhara, Abdulla Al Masum, Saugata Konar, Saikat Kumar Manna, Saikat Kumar Seth, Sudipta Pathak, Subrata Mukhopadhyay
A terpyridine based compound L1 was designed and synthesized as an "off-on" chemosensor for the detection of Zn2+. Chemosensor L1 showed excellent selectivity and sensitivity toward Zn2+ by exhibiting a large fluorescence enhancement (∼51-fold) at 370 nm whereas other competitive metal ions did not show any noticeable change in the emission spectra of chemosensor L1. The chemosensor (L1) was shown to detect Zn2+ ions down to 9.76 μM at pH 7.4. However, chemosensor L1 binds Zn2+ in a 1 : 2 ratio (receptor : metal) with an association constant of 1...
June 20, 2018: Photochemical & Photobiological Sciences
Young In Lee, Ju Hee Lee, Do Young Kim, Kee Yang Chung, Jung U Shin
BACKGROUND: Recent reports have suggested that the topical formulation of sirolimus is effective in treating facial angiofibromas in tuberous sclerosis complex (TSC). Here, we determined the safety and efficacy of 0.2% topical sirolimus for the treatment of facial angiofibroma and compared its effects based on age. METHOD: This was a retrospective study which involved 36 TSC patients with facial angiofibromas who were treated with 0.2% sirolimus ointment. Its effect was evaluated using the Facial Angiofibroma Severity Index (FASI)...
June 20, 2018: Dermatology: International Journal for Clinical and Investigative Dermatology
C R M de Lima, D N Gomes, J R de Morais Filho, M R Pereira, J L C Fonseca
Interpolyelectrolyte complexes of chitosan and poly(sodium 4-styrenesulfonate) [NaPSS] were synthesized and obtained in the form of solid particles, with two different sulfonate to aminium molar ratios: 0.7, resulting in particles with positive zeta potential (IPEC+ ), and 1.4, yielding particles with negative zeta potential (IPEC- ). Both particles were characterized as potential drug sorbents using differently charged drugs: sodium cromoglycate (negatively charged), and tetracycline hydrochloride (positively charged)...
June 15, 2018: Colloids and Surfaces. B, Biointerfaces
Yue-Yue Chang, Hai-Long Wu, Huan Fang, Tong Wang, Zhi Liu, Yang-Zi Ouyang, Yu-Jie Ding, Ru-Qin Yu
In this study, a smart and green analytical method based on the second-order calibration algorithm coupled with excitation-emission matrix (EEM) fluorescence was developed for the determination of rhodamine dyes illegally added into chilli samples. The proposed method not only has the advantage of high sensitivity over the traditional fluorescence method but also fully displays the "second-order advantage". Pure signals of analytes were successfully extracted from severely interferential EEMs profiles via using alternating trilinear decomposition (ATLD) algorithm even in the presence of common fluorescence problems such as scattering, peak overlaps and unknown interferences...
June 15, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Jeong Hwan Park, Cheol Lee, Mee Soo Chang, Kwangsoo Kim, Seongmin Choi, Hyunjung Lee, Hyun-Seob Lee, Kyung Chul Moon
Tuberous sclerosis complex-associated renal cell carcinoma (TSC-RCC) has distinct clinical and histopathologic features and is considered a specific subtype of RCC. The genetic alterations of TSC1 or TSC2 are responsible for the development of TSC. In this study, we assessed the mTOR pathway activation and aimed to evaluate molecular characteristics and pathogenic pathways of TSC-RCC. Two cases of TSC-RCC, one from a 31-year-old female and the other from an 8-year-old male, were assessed. The mTOR pathway activation was determined by immunohistochemistry...
June 17, 2018: Translational Oncology
Thomas Schmitt, Stefan Lange, Stefan Sonnenberger, Bodo Dobner, Bruno Demé, Andreas Langner, Reinhard H H Neubert
This study used neutron diffraction to investigate a ceramide-[NP] C24/[AP] C24 /[EOS]-br C30/cholesterol/lignoceric acid (0.6: 0.3: 0.1: 0.7: 1) based stratum corneum modelling system. By adding specifically deuterated ceramides-[NP]-D3 , [AP]-D3 , and [EOS]-br-D3 , detailed information on the lamellar and the nanostructure of the system was obtained. For the short periodicity phase a natural-like lamellar repeat distance of 5.47 ± 0.02 nm was observed, similar to the [NP]/[AP] base system without the [EOS]-br...
June 17, 2018: Biochimica et Biophysica Acta
Rebecca L Maher, Michelle A Johnston, Marilyn E Brandt, Tyler B Smith, Adrienne M S Correa
Bioerosion, the removal of calcium carbonate from coral frameworks by living organisms, influences a variety of reef features, from their topographic complexity to the net balance of carbonate budgets. Little is known, however, about how macroborers, which bore into reef substrates leaving traces greater than 0.1 mm diameter, are distributed across coral reefs, particularly reef systems with high (>50%) stony coral cover or at mesophotic depths (≥30 m). Here, we present an accurate and efficient method for quantifying macroborer densities from stony coral hosts via image analysis, using the bioeroding barnacle, Lithotrya dorsalis, and its host coral, Orbicella franksi, as a case study...
2018: PloS One
Ryan A McManamay, Matthew J Troia, Christopher R DeRolph, Arlene Olivero Sheldon, Analie R Barnett, Shih-Chieh Kao, Mark G Anderson
Describing the physical habitat diversity of stream types is important for understanding stream ecosystem complexity, but also prioritizing management of stream ecosystems, especially those that are rare. We developed a stream classification system of six physical habitat layers (size, gradient, hydrology, temperature, valley confinement, and substrate) for approximately 1 million stream reaches within the Eastern United States in order to conduct an inventory of different types of streams and examine stream diversity...
2018: PloS One
S G Kozlov, L Sh Khamchieva, O A Pogorelova, M I Tripoten', T V Balakhonova
The study was aimed at assessing the dynamics of asymptomatic atherosclerosis of carotid arteries (CA) depending upon the achieved level of low-density lipoprotein cholesterol (LDLC) in patients with moderate total risk by the SCORE scale. We followed up a total of eighty-two 40-to-65-year-old patients with the LDLC level above 2.6 mmol/l, being at moderate total risk by the SCORE scale and having symptom-free atherosclerosis of the extracranial portion of brachiocephalic arteries (up to 50% narrowing of their lumen) as diagnosed by duplex scanning...
2018: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
Alzira Alves de Siqueira Carvalho, Emmanuele Lacene, Guy Brochier, Clémance Labasse, Angeline Madelaine, Vinicius Gomes da Silva, Roseli Corazzini, Konstantinos Papadopoulos, Anthony Behin, Pascal Laforêt, Tania Stojkovic, Bruno Eymard, Michel Fardeau, Norma Romero
BACKGROUND: Protein aggregate myopathies (PAM) represent a group of familial or sporadic neuromuscular conditions with marked clinical and genetic heterogeneity that occur in children and adults. Familial PAM includes myofibrillar myopathies defined by the presence of desmin-positive protein aggregates and degenerative intermyofibrillar network changes. PAM is often caused by dysfunctional genes, such as DES, PLEC 1, CRYAB, FLNC, MYOT, ZASP, BAG3, FHL1, and DNAJB6. OBJECTIVE: To retrospectively analyze genetic mutations and demographic, clinical, and morphological aspects of PAM in a French population...
June 2018: Genetic Testing and Molecular Biomarkers
Anil Annadi, Guanglei Cheng, Hyungwoo Lee, Jung-Woo Lee, Shicheng Lu, Anthony Tylan-Tyler, Megan Briggeman, Michelle Tomczyk, Mengchen Huang, David Pekker, Chang-Beom Eom, Patrick Irvin, Jeremy Levy
SrTiO3 -based heterointerfaces support quasi-two-dimensional (2D) electron systems that are analogous to III-V semiconductor heterostructures, but also possess superconducting, magnetic, spintronic, ferroelectric, and ferroelastic degrees of freedom. Despite these rich properties, the relatively low mobilities of 2D complex-oxide interfaces appear to preclude ballistic transport in 1D. Here we show that the 2D LaAlO3 /SrTiO3 interface can support quantized ballistic transport of electrons and (non-superconducting) electron pairs within quasi-1D structures that are created using a well-established conductive atomic-force microscope (c-AFM) lithography technique...
June 20, 2018: Nano Letters
Nicholas M Riley, Jacek W Sikora, Henrique S Seckler, Joseph B Greer, Ryan T Fellers, Richard D LeDuc, Michael S Westphall, Paul M Thomas, Neil L Kelleher, Joshua J Coon
High-throughput top-down proteomic experiments directly identify proteoforms in complex mixtures, making high quality tandem mass spectra necessary to deeply characterize proteins with many sources of variation. Collision-based dissociation methods offer expedient data acquisition, but often fail to extensively fragment proteoforms for thorough analysis. Electron-driven dissociation methods are a popular alternative approach, especially for precursor ions with high charge density. Combining infrared photo-activation concurrent with electron transfer dissociation (ETD) reactions, i...
June 20, 2018: Analytical Chemistry
L M M Oliveira, M V A Amorim, C A Corsini, C C A Neto, D G Chaves
INTRODUCTION: Diagnosis of von Willebrand disease (VWD) is difficult due to the heterogenic phenotype of patients and to the complex tests that are required for an adequate investigation. The collagen binding assay (VWF:CB) reveals the adhesion capacity of von Willebrand factor (VWF) to collagen and can be useful to reduce the misleading diagnosis of VWD. This study aimed the standardization of 2 nonautomated VWF:CB assays based on ELISA and flow cytometry. METHODS: Plasma samples from 87 patients previously diagnosed with VWD and 22 healthy controls were analyzed...
June 20, 2018: International Journal of Laboratory Hematology
Michael P Triebwasser, Xiaobo Wu, Paula Bertram, Dennis E Hourcade, Donald Michael Nelson, John P Atkinson
PROBLEM: Crry is a widely expressed type 1 transmembrane complement regulatory protein in rodents which protects self-tissue by downregulating C3 activation. Crry-/- concepti produced by Crry+/-  × Crry+/- matings are attacked by maternal complement system leading to loss before day 10. The membrane attack complex is not the mediator of this death. We hypothesized that the ability of C3b to engage the alternative pathway's feedback loop relatively unchecked on placental membranes induces the lesion yielding the demise of the Crry-/- mouse...
June 20, 2018: American Journal of Reproductive Immunology: AJRI
Eric Fombonne
The first autism surveys were simple head counts of children already diagnosed with a severe autism phenotype and residing in small, circumscribed geographical areas. Prevalence was low, ranging from 0.4 to 2/1,000 in the 1960's and 1970's. Today, the methodology of surveys has become more complex; studies include large populations, multiple sites, stratified samples and rely on intricate sets of screening activities followed by some form of diagnostic confirmation procedures. Yet, and as surprising as it may be, there is no standardization of autism survey methodology...
July 2018: Journal of Child Psychology and Psychiatry, and Allied Disciplines
X Averós, I Estevez
To better understand how relevant intensive systems' characteristics simultaneously affect the performance and welfare of broiler chickens, a meta-analysis of recent literature was carried out. The study determined the effects of gender, genetics, experimental initial age (EIA, d), stocking density (SD; kg/m2), group size (GS; n), bedding material (yes/no), duration of photoperiod (DP; h), divided scotoperiod (yes/no), feeding phases (1/2/3/>3), environmental control (EC; yes/no), environmental enrichment (yes/no), use of vaccines and other medications (yes/no), experimental duration (d), and relevant 2-way interactions on average daily gain (g/d), average daily feed intake (g/d), FCR (g: g), mortality (%), behavior (%), and gait score (mean value)...
June 19, 2018: Poultry Science
Wioleta Drobik-Czwarno, Anna Wolc, Janet E Fulton, Tomasz Jankowski, Jesus Arango, Neil P O'Sullivan, Jack C M Dekkers
An outbreak of H5N2 highly pathogenic avian influenza (HPAI) in 2015, resulting in mandatory euthanization of millions of chickens, was one of the most fatal in the US history. The aim of this study was to detect genes associated with survival following natural infection with HPAI during this outbreak. Blood samples were collected from 274 individuals from 3 commercial varieties of White Leghorn. Survivors and age and genetics matched non-affected controls from each variety were included in the comparison. All individuals were genotyped on the 600k SNP array...
June 19, 2018: Poultry Science
Moritz Bömer, José A O'Brien, Imma Pérez-Salamó, Jovaras Krasauskas, Paul Finch, Andrea Briones, Arsalan Daudi, Puneet Souda, Tjir-Li Tsui, Julian P Whitelegge, G Paul Bolwell, Alessandra Devoto
Background and Aims: Cultured cell suspensions have been the preferred model to study the apoplast as well as to monitor metabolic and cell cycle-related changes. Previous work showed that methyl jasmonate (MeJA) inhibits leaf growth in a CORONATINE INSENSITIVE 1 (COI1)-dependent manner, with COI1 being the jasmonate (JA) receptor. Here, the effect of COI1 overexpression on the growth of stably transformed arabidopsis cell cultures is described. Methods: Time-course experiments were carried out to analyse gene expression, and protein and metabolite levels...
June 19, 2018: Annals of Botany
Laurino Carmen, Vadala Maria, Julio Cesar Morales-Medina, Annamaria Vallelunga, Beniamino Palmieri, Tommaso Iannitti
Duchenne Muscular dystrophy (DMD) is an inherited fatal X-linked myogenic disorder with a prevalence of 1 in 3500 male live births. It affects voluntary muscles, and heart and breathing muscles. DMD is characterised by continuous degeneration and regeneration cycles resulting in extensive fibrosis and a progressive reduction in muscle mass. Since the identification of a reduction in dystrophin protein as the cause of this disorder, numerous innovative and experimental therapies, focusing on increasing the levels of dystrophin, have been proposed, but the clinical improvement has been unsatisfactory...
June 19, 2018: Glycobiology
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