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Pyrroloquinoline

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https://www.readbyqxmd.com/read/28578007/sensitivity-of-hips-derived-neural-stem-cells-nsc-to-pyrroloquinoline-quinone-depends-on-their-developmental-stage
#1
J Augustyniak, J Lenart, M Zychowicz, G Lipka, P Gaj, M Kolanowska, P P Stepien, L Buzanska
Pyrroloquinoline quinone (PQQ) is a factor influencing on the mitochondrial biogenesis. In this study the PQQ effect on viability, total cell number, antioxidant capacity, mitochondrial biogenesis and differentiation potential was investigated in human induced Pluripotent Stem Cells (iPSC) - derived: neural stem cells (NSC), early neural progenitors (eNP) and neural progenitors (NP). Here we demonstrated that sensitivity to PQQ is dependent upon its dose and neural stage of development. Induction of the mitochondrial biogenesis by PQQ at three stages of neural differentiation was evaluated at mtDNA, mRNA and protein level...
May 31, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28572558/synthesis-and-crystal-structure-of-pyrroloquinoline-quinol-pqqh2-and-pyrroloquinoline-quinone-pqq
#2
Kazuto Ikemoto, Shigeki Mori, Kazuo Mukai
Pyrroloquinoline quinone (PQQ) is a water-soluble quinone compound first identified as a cofactor of alcohol- and glucose-dehydrogenases (ADH and GDH) in bacteria. For example, in the process of ADH reaction, alcohol is oxidized to the corresponding aldehyde, and inversely PQQ is reduced to pyrroloquinoline quinol (PQQH2). PQQ and PQQH2 molecules play an important role as a cofactor in ADH and GDH reactions. However, crystal structure analysis has not been performed for PQQ and PQQH2. In the present study, the synthesis of PQQH2 powder crystals was performed under air, by utilizing vitamin C as a reducing agent...
June 1, 2017: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
https://www.readbyqxmd.com/read/28481092/nuclear-magnetic-resonance-structure-and-binding-studies-of-pqqd-a-chaperone-required-in-the-biosynthesis-of-the-bacterial-dehydrogenase-cofactor-pyrroloquinoline-quinone
#3
Robert L Evans, John A Latham, Youlin Xia, Judith P Klinman, Carrie M Wilmot
Biosynthesis of the ribosomally synthesized and post-translationally modified peptide (RiPP), pyrroloquinoline quinone (PQQ), is initiated when the precursor peptide, PqqA, is recognized and bound by the RiPP precursor peptide recognition element (RRE), PqqD, for presentation to the first enzyme in the pathway, PqqE. Unlike other RiPP-producing, postribosomal peptide synthesis (PRPS) pathways in which the RRE is a component domain of the first enzyme, PqqD is predominantly a separate scaffolding protein that forms a ternary complex with the precursor peptide and first tailoring enzyme...
May 30, 2017: Biochemistry
https://www.readbyqxmd.com/read/28469179/highly-selective-and-sensitive-self-powered-glucose-sensor-based-on-capacitor-circuit
#4
Gymama Slaughter, Tanmay Kulkarni
Enzymatic glucose biosensors are being developed to incorporate nanoscale materials with the biological recognition elements to assist in the rapid and sensitive detection of glucose. Here we present a highly sensitive and selective glucose sensor based on capacitor circuit that is capable of selectively sensing glucose while simultaneously powering a small microelectronic device. Multi-walled carbon nanotubes (MWCNTs) is chemically modified with pyrroloquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BOD) at anode and cathode, respectively, in the biofuel cell arrangement...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28448745/medium-optimization-for-pyrroloquinoline-quinone-pqq-production-by-methylobacillus-sp-zju323-using-response-surface-methodology-and-artificial-neural-network-genetic-algorithm
#5
Peilian Wei, Zhenjun Si, Yao Lu, Qingfei Yu, Lei Huang, Zhinan Xu
Methylobacillus sp. zju323 was adopted to improve the biosynthesis of pyrroloquinoline quinone (PQQ) by systematic optimization of the fermentation medium. The Plackett-Burman design was implemented to screen for the key medium components for the PQQ production. CoCl2 · 6H2O, ρ-amino benzoic acid, and MgSO4 · 7H2O were found capable of enhancing the PQQ production most significantly. A five-level three-factor central composite design was used to investigate the direct and interactive effects of these variables...
April 27, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28407913/pyrroloquinoline-quinone-from-gluconobacter-oxydans-fermentation-broth-enhances-superoxide-anion-scavenging-capacity-of-cu-zn-sod
#6
Ke Ma, Jun-Zhu Cui, Jian-Bin Ye, Xian-Mei Hu, Ge-Li Ma, Xue-Peng Yang
A bioassay-guided fractionation of extract from Gluconobacter oxydans fermentation broth afforded Compound 1, which was identified as pyrroloquinoline quinone (PQQ) by spectroscopic methods. PQQ has been shown to enhance the superoxide anion-scavenging capacity significantly for Cu/Zn-SOD. To illustrate the mechanism, the interaction between PQQ and Cu/Zn-SOD was investigated. The multiple binding sites involving hydrogen bonds and van der Waals force between PQQ and Cu/Zn-SOD were revealed by isothermal titration calorimetry...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28386349/pyrroloquinoline-quinone-prevents-testosterone-deficiency-induced-osteoporosis-by-stimulating-osteoblastic-bone-formation-and-inhibiting-osteoclastic-bone-resorption
#7
Xuan Wu, Jie Li, Hengwei Zhang, Hui Wang, Guoyong Yin, Dengshun Miao
Accumulating evidences suggest that oxidative stress caused and deteriorated the aging related osteoporosis and pyrroloquinoline quinone (PQQ) is a powerful antioxidant. However, it is unclear whether PQQ can prevent testosterone deficiency-induced osteoporosis. In this study, the orchidectomized (ORX) mice were supplemented in diet with/without PQQ for 48 weeks, and compared with each other and with sham mice. Results showed that bone mineral density, trabecular bone volume, collagen deposition and osteoblast number were decreased significantly in ORX mice compared with shame mice, whereas PQQ supplementation largely prevented these alterations...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28374050/enhanced-fed-batch-production-of-pyrroloquinoline-quinine-in-methylobacillus-sp-cctcc-m2016079-with-a-two-stage-ph-control-strategy
#8
Zhenjun Si, David Machaku, Peilian Wei, Lei Huang, Jin Cai, Zhinan Xu
The effects of pH control strategy and fermentative operation modes on the biosynthesis of pyrroloquinoline quinine (PQQ) were investigated systematically with Methylobacillus sp. CCTCC M2016079 in the present work. Firstly, the shake-flask cultivations and benchtop fermentations at various pH values ranging from 5.3 to 7.8 were studied. Following a kinetic analysis of specific cell growth rate (μ x ) and specific PQQ formation rate (μ p ), the discrepancy in optimal pH values between cell growth and PQQ biosynthesis was observed, which stimulated us to develop a novel two-stage pH control strategy...
April 3, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28344084/a-rapid-procedure-for-the-in-situ-assay-of-periplasmic-pqq-dependent-methanol-dehydrogenase-in-intact-single-bacterial-colonies
#9
Venkata Ramana Vemuluri, Shreya Shaw, Caroline Autenrieth, Robin Ghosh
Mechanistic details of methanol oxidation catalyzed by the periplasmically-located pyrroloquinoline quinone-dependent methanol dehydrogenase of methylotrophs can be elucidated using site-directed mutants. Here, we present an in situ colony assay of methanol dehydrogenase, which allows robotic screening of large populations of intact small colonies, and regrowth of colonies for subsequent analysis.
March 23, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28155960/connecting-quantum-dots-with-enzymes-mediator-based-approaches-for-the-light-directed-read-out-of-glucose-and-fructose-oxidation
#10
M Riedel, N Sabir, F W Scheller, W J Parak, F Lisdat
The combination of the biocatalytic features of enzymes with the unique physical properties of nanoparticles in a biohybrid system provides a promising approach for the development of advanced bioelectrocatalytic devices. This study describes the construction of photoelectrochemical signal chains based on CdSe/ZnS quantum dot (QD) modified gold electrodes as light switchable elements, and low molecular weight redox molecules for the combination with different biocatalysts. Photoelectrochemical and photoluminescence experiments verify that electron transfer can be achieved between the redox molecules hexacyanoferrate and ferrocene, and the QDs under illumination...
February 3, 2017: Nanoscale
https://www.readbyqxmd.com/read/28124619/the-effect-of-pyrroloquinoline-quinone-on-apoptosis-and-autophagy-in-traumatic-brain-injury
#11
Pengju Zhang, Yongqi Ye, Yuhang Qian, Baoxin Yin, Jianmei Zhao, Shunxin Zhu, Li Zhang, Meijuan Yan
BACKGROUND: Pyrroloquinoline quinoe is an anionic, water-soluble compound with antioxidant characteristic. The role of pyrroloquinoline quinoe in pharmacology and nutrition has attracted wide attention of researchers. Although a few experiments have confirmed that pyrroloquinoline quinoe plays an obvious effective role in neuroprotection. There are little reports about the effect of pyrroloquinoline quinoe in traumatic brain injury. Traumatic brain injury is one of the leading causes of adult disability and death in the world...
January 24, 2017: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/28115068/the-role-of-oxidative-stress-in-dna-damage-in-pancreatic-%C3%AE-cells-induced-by-di-2-ethylhexyl-phthalate
#12
Yan She, Liping Jiang, Liangliang Zheng, Heming Zuo, Min Chen, Xiance Sun, Qiujuan Li, Chengyan Geng, Guang Yang, Lijie Jiang, Xiaofang Liu
Di(2-ethyhexyl) phthalate (DEHP) is commonly used as a plasticizer, which loosely binds to plastic materials and easily leaches out of these products and enters into the environment. Exposure to DEHP can impair pancreatic beta cells (INS-1 cells)function, which is associated with Insulin Resistance (IR) and type 2 diabetes. However, the mechanism of how DEHP leads to Insulin Resistance is unknown. Our results showed that the cell viability of INS-1 cells exposed to DEHP (0-1600 μM) were decreased in a concentration-dependent manner...
March 1, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28105098/radioprotective-effects-of-pyrroloquinoline-quinone-on-parotid-glands-in-c57bl-6j-mice
#13
Yuanqing Huang, Ning Chen, Dengshun Miao
The aim of the present study was to investigate whether pyrroloquinoline quinine (PQQ) serve a radioprotective role in parotid gland damage induced by total body irradiation (TBI) in C57BL/6J mice. A total of 15 female 8-week-old C57BL/6J mice were randomly assigned into three treatment groups: i) Untreated control (no irradiation); ii) 4 gray (Gy) X-ray irradiation; iii) 4 Gy X-ray irradiation with additional dietary PQQ (4 mg PQQ/kg in normal diet). Each group included five mice. After 4 weeks, all animals were collected for evaluating the phenotype, body weight, pathological and biochemical parameters...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28063921/tricalcium-phosphate-solubilization-and-nitrogen-fixation-by-newly-isolated-aneurinibacillus-aneurinilyticus-ckmv1-from-rhizosphere-of-valeriana-jatamansi-and-its-growth-promotional-effect
#14
Anjali Chauhan, Shiwani Guleria, Praveen P Balgir, Abhishek Walia, Rishi Mahajan, Preeti Mehta, Chand Karan Shirkot
Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260mg/L), nitrogen fixation (202.91nmolethylenemL(-1)h(-1)), indole-3-acetic acid (IAA) (8.1μg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1...
April 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28009976/ammosamides-unveil-novel-biosynthetic-machinery
#15
Dominic A Colosimo, John B MacMillan
In this issue of Cell Chemical Biology, Jordan and Moore (2016) present a thorough biosynthetic analysis of ammosamides, a bacterial natural product. The work highlights the previously unknown overlap between two natural products families: pyrroloquinoline alkaloids and ribosomally synthesized posttranslationally modified peptides (RiPPs).
December 22, 2016: Cell Chemical Biology
https://www.readbyqxmd.com/read/28007783/early-pqq-supplementation-has-persistent-long-term-protective-effects-on-developmental-programming-of-hepatic-lipotoxicity-and-inflammation-in-obese-mice
#16
Karen R Jonscher, Michael S Stewart, Alba Alfonso-Garcia, Brian C DeFelice, Xiaoxin X Wang, Yuhuan Luo, Moshe Levi, Margaret J R Heerwagen, Rachel C Janssen, Becky A de la Houssaye, Ellen Wiitala, Garrett Florey, Raleigh L Jonscher, Eric O Potma, Oliver Fiehn, Jacob E Friedman
Nonalcoholic fatty liver disease (NAFLD) is widespread in adults and children. Early exposure to maternal obesity or Western-style diet (WD) increases steatosis and oxidative stress in fetal liver and is associated with lifetime disease risk in the offspring. Pyrroloquinoline quinone (PQQ) is a natural antioxidant found in soil, enriched in human breast milk, and essential for development in mammals. We investigated whether a supplemental dose of PQQ, provided prenatally in a mouse model of diet-induced obesity during pregnancy, could protect obese offspring from progression of NAFLD...
April 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27951709/amphiphilic-polymer-mediators-promoting-electron-transfer-on-bioanodes-with-pqq-dependent-glucose-dehydrogenase
#17
Yasuo Nakashima, Norihiro Mizoshita, Hiromitsu Tanaka, Yuichiro Nakaoki
Redox-active phenazinium salts bonded to amphiphilic polymer backbones are demonstrated to function as high-performance electron-transfer mediators in enzymatic bioanodes applicable to biofuel cells. The redox-active moieties could be easily tethered to the electrodes by physical adsorption of the hydrophobic regions of the polymer backbones onto the electrode surface. On the other hand, long hydrophilic chains were essential to ensure high mobility of the redox-active moieties in aqueous solutions and to enhance their electron-transfer properties...
December 13, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27866908/biosynthetic-pathway-connects-cryptic-ribosomally-synthesized-posttranslationally-modified-peptide-genes-with-pyrroloquinoline-alkaloids
#18
Peter A Jordan, Bradley S Moore
In an era where natural product biosynthetic gene clusters can be rapidly identified from sequenced genomes, it is unusual for the biosynthesis of an entire natural product class to remain unknown. Yet, the genetic determinates for pyrroloquinoline alkaloid biosynthesis have remained obscure despite their abundance and deceptive structural simplicity. In this work, we have identified the biosynthetic gene cluster for ammosamides A-C, pyrroloquinoline alkaloids from Streptomyces sp. CNR-698. Through direct cloning, heterologous expression and gene deletions we have validated the ammosamide biosynthetic gene cluster and demonstrated that these seemingly simple molecules are derived from a surprisingly complex set of biosynthetic genes that are also found in the biosynthesis of lymphostin, a structurally related pyrroloquinoline alkaloid from Salinispora and Streptomyces...
December 22, 2016: Cell Chemical Biology
https://www.readbyqxmd.com/read/27836608/an-improved-glycerol-biosensor-with-an-au-fes-nad-glycerol-dehydrogenase-anode
#19
Aishwarya Mahadevan, Sandun Fernando
An improved glycerol biosensor was developed via direct attachment of NAD(+)-glycerol dehydrogenase coenzyme-apoenzyme complex onto supporting gold electrodes, using novel inorganic iron (II) sulfide (FeS)-based single molecular wires. Sensing performance factors, i.e., sensitivity, a detection limit and response time of the FeS and conventional pyrroloquinoline quinone (PQQ)-based biosensor were evaluated by dynamic constant potential amperometry at 1.3V under non-buffered conditions. For glycerol concentrations ranging from 1 to 25mM, a 77% increase in sensitivity and a 53% decrease in detection limit were observed for the FeS-based biosensor when compared to the conventional PQQ-based counterpart...
June 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27760187/genetically-engineered-escherichia-coli-nissle-1917-synbiotics-reduce-metabolic-effects-induced-by-chronic-consumption-of-dietary-fructose
#20
Chaudhari Archana Somabhai, Ruma Raghuvanshi, G Nareshkumar
AIMS: To assess protective efficacy of genetically modified Escherichia coli Nissle 1917 (EcN) on metabolic effects induced by chronic consumption of dietary fructose. MATERIALS AND METHODS: EcN was genetically modified with fructose dehydrogenase (fdh) gene for conversion of fructose to 5-keto-D-fructose and mannitol-2-dehydrogenase (mtlK) gene for conversion to mannitol, a prebiotic. Charles foster rats weighing 150-200 g were fed with 20% fructose in drinking water for two months...
2016: PloS One
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