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DNA silver nanocluster, protein detection

Xiaoru Zhang, Fengting Chen, Xiaojie Song, Peng He, Shusheng Zhang
A novel method for study the interaction between carbohydrate and lectin was developed in homogenous solution. DNA was introduced to the recognition process of carbohydrate with protein. Due to the versatile detection mode of DNA, the detection efficiency for carbohydrate and protein was highly promoted. Herein, two kinds of mannose-DNA conjugates were synthesized. One of which was used to prepare DNA-templated silver nanocluster (AgNCs) and the other one contained guanine-rich DNA sequences. When the mannose of two conjugates binding to lectin Concanavalin A (Con A), great enhancement on fluorescence intensity was obtained due to the proximity ligation of the DNA-templated Ag NCs with guanine-rich DNA sequences...
May 1, 2018: Biosensors & Bioelectronics
Zhikun Zhang, Yang Liu, Peifeng Liu, Lu Yang, Xingyu Jiang, Dan Luo, Dayong Yang
Chirality plays essential roles in life systems such that l-amino acids (LAAs) are predominantly found as the building units of protein for organisms. The presence of the d-enantiomer (DAA) has been found to be specifically relevant to gastric cancer. We herein construct a luminescent DNA/silver nanocluster based biosensing system to achieve rapid and specific detection of DAAs. As a proof of application, we detected DAAs in saliva samples from patients with gastric cancer, and the test results exhibited excellent specificity...
December 14, 2017: Nanoscale
Zhenjun Liu, Wansong Chen, Yajin Han, Jiang Ouyang, Min Chen, Shengqiang Hu, Liu Deng, You-Nian Liu
Recently, membrane proteins have been considered as candidate cancer biomarkers and drug targets, due to their important roles in numerous physiological processes. Therefore, a facile, sensitive and quantitative detection of the membrane proteins is crucial for better understanding their roles in cancer cells and further validating their function in clinical research. We report a highly facile and sensitive detection method for membrane proteins on living cells in situ based on membrane protein-triggered release of cytosine (C)-rich single-stranded DNA (ssDNA) sequences, and the subsequent silver nanoclusters (AgNCs) transfer from polymer to C-rich ssDNA...
December 1, 2017: Talanta
Wei-Xian Wang, Yee-Wai Cheung, Roderick M Dirkzwager, Wai-Chung Wong, Julian A Tanner, Hong-Wei Li, Yuqing Wu
Innovative nanomaterials offer significant potential for diagnosis of severe diseases of the developing world such as malaria. Small sized silver nanoclusters have shown promise for diagnostics due to their intense fluorescence emission and photo-stabilities. Here, double-stranded DNA-scaffolded silver nanoclusters (AgNCs-dsDNA) were prepared to detect the established malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). Significant luminescence enhancement over a wide concentration range of PfLDH was demonstrated...
January 31, 2017: Analyst
Guoliang Liu, Jingjing Li, Da-Qian Feng, Jun-Jie Zhu, Wei Wang
An activatable silver nanoclusters beacon (ASNCB) was synthesized through a facile one-pot approach and applied for multiplex DNAs, small molecule, and protein sensing. Multifunctional single-stranded DNA sequences are rationally designed and used for ASNCB in situ synthesis. Via target-responsive structure transformation of ASNCB, target recognition induced ASNCB conformational transition and lit up the fluorescent signal of silver nanoclusters. By further implementing two different color ASNCBs (520 and 600 nm), the parallel multiplexed analysis of two target genes (Influenza A virus genes H1N1 and H5N1) is achieved...
January 3, 2017: Analytical Chemistry
Mojtaba Shamsipur, Fatemeh Molaabasi, Saman Hosseinkhani, Fereshteh Rahmati
Cytochrome c (Cyt c) is an important biomarker in cell lysates for the early stage of apoptosis or anticancer agents. Here, two novel label-free fluorescence assays based on hemoglobin-stabilized gold nanoclusters (Hb/AuNCs) and aptamer-stabilized silver nanoclusters (DNA/AgNCs) for analysis of Cyt c are presented. The heme group of the protein induces sensitive sensing platforms accompanied by the decreased fluorescence of both metal nanoclusters. The quenching processes observed found to be based on the fluorescence resonance energy transfer mechanism from Hb/AuNCs to Cyt c and photoinduced electron transfer from DNA/AgNCs to the aptamer-Cyt c complex...
February 16, 2016: Analytical Chemistry
Jiamian Wang, Xiuyun Wang, Shuo Wu, Jie Song, Yanqiu Zhao, Yanqiu Ge, Changgong Meng
Silver nanoclusters and graphene oxide nanocomposite (AgNCs/GRO) is synthesized and functionalized with detection antibody for highly sensitive electrochemical sensing of carcinoembryonic antigen (CEA), a model tumor marker involved in many cancers. AgNCs with large surface area and abundant amount of low-coordinated sites are synthesized with DNA as template and exhibit high catalytic activity towards the electrochemical reduction of H2O2. GRO is employed to assemble with AgNCs because it has large specific surface area, super electronic conductivity and strong π-π stacking interaction with the hydrophobic bases of DNA, which can further improve the catalytic ability of the AgNCs...
February 4, 2016: Analytica Chimica Acta
Bing Zhang, Bingqian Liu, Jun Zhou, Juan Tang, Dianping Tang
A new signal-on immunoassay protocol for sensitive electronic detection of alpha-fetoprotein (AFP) was developed by coupling with metal sulfide nanolabels and a silver nanocluster (AgNC)-based rolling circle amplification (RCA) strategy. Initially, a sandwiched immunocomplex was formed on a primary antibody-coated microplate using a PbS nanoparticle-labeled polyclonal anti-AFP antibody (PbS-pAb2) as the detection antibody, and then the carried PbS-pAb2 was dissolved by acid to release a large number of lead ions, which could induce the cleavage of lead-specific DNAzyme immobilized on the electrode...
May 22, 2013: ACS Applied Materials & Interfaces
Ya Wen Zhou, Chun Mei Li, Yue Liu, Cheng Zhi Huang
Silver nanoclusters (AgNCs) have gained considerable attention over a wide range from chemical detection to bioimaging applications. Herein we developed a facile way to synthesize water-soluble, intense fluorescent targetable AgNCs with yellow-emission by using DNA via a chemical reduction method. The sequence of DNA was designed to contain two fragments, one of which is the DNA aptamer fragment which can selectively bind to the target, and the other is rich in cytosine sequence fragments that can result in a high quantum efficiency...
February 21, 2013: Analyst
Jing-Jing Liu, Xiao-Rong Song, Yi-Wei Wang, Ai-Xian Zheng, Guo-Nan Chen, Huang-Hao Yang
A simple turn-on and homogeneous aptasensor, which relies on target induced formation of silver nanoclusters (Ag NCs), was developed for the determination of platelet-derived growth factor B-chain homodimer (PDGF-BB). The aptasensor contains two hairpin DNA probes termed as P1 and P2. P1 consists of the aptamer sequence of PDGF-BB. Meanwhile, P2 contains the Ag NCs nucleation sequence, which is blocked by the hairpin stem region. P1 and P2 can co-exist metastably in the absence of PDGF-BB and maintain hairpin structure...
October 24, 2012: Analytica Chimica Acta
Yanan Wang, Jing Zhang, Lingyun Huang, Dacheng He, Lin Ma, Jin Ouyang, Fubin Jiang
We have developed a novel application for DNA oligonucleotide-stabilized Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE). Oligonucleotide-stabilized Ag nanoclusters were used as fluorescent probes for direct detection of proteins after native PAGE. Some relatively low-abundance proteins, such as α-1-antichymotrypsin (ACT) and α-2-glycoprotein 1, zinc (ZAG) were easily detected by oligonucleotide-stabilized Ag nanocluster-based fluorescent imaging and identified by MS and MS/MS techniques, without the need of expensive antibodies or tedious immunoassay procedures...
January 27, 2012: Chemistry: a European Journal
Bingyan Han, Erkang Wang
In this review, we discuss the synthesis and applications of DNA-templated fluorescent silver nanoclusters in aqueous solution. Various oligonucleotide sequences or conformations have been utilized to synthesize silver nanoclusters with excellent fluorescence properties. The range of applications has expanded greatly, from live cell staining and the detection of metal ions and small biomolecules to the detection of DNA or proteins.
January 2012: Analytical and Bioanalytical Chemistry
Guo-Yu Lan, Wei-Yu Chen, Huan-Tsung Chang
A simple strategy for the preparation of strongly fluorescent and stable DNA-Cu/Ag NCs from reduction of AgNO(3) and Cu(NO(3))(2) by NaBH(4) in the presence of DNA having a sequence 5'-CCCTTAATCCCC-3' has been demonstrated. Fluorescence, absorption, X-ray photoelectron spectroscopy (XPS), and electrospray ionization-mass spectrometry (ESI-MS) measurements have been applied to the characterization of the DNA-Cu/Ag NCs. The ESI-MS data reveal that each DNA-Cu/Ag NC contained 2 Ag and 1 Cu atoms. The interactions among DNA with the Ag and Cu atoms are further supported by the data of low-temperature fluorescence...
September 21, 2011: Analyst
Jaswinder Sharma, Hsin-Chih Yeh, Hyojong Yoo, James H Werner, Jennifer S Martinez
We have synthesized an intrinsically fluorescent recognition ligand that combines the high fluorescence quantum yield (>50%) of oligonucleotide templated AgNCs with the specificity and strong binding affinity of DNA aptamers for their target proteins, to develop a new strategy for detection of specific proteins.
February 28, 2011: Chemical Communications: Chem Comm
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