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Felix J F Herth, Ralf Eberhardt, Daniel Sterman, Gerard A Silvestri, Hans Hoffmann, Pallav L Shah
INTRODUCTION: The promise of benefits from lung cancer screening is tempered by the false positive rate and the need to perform diagnostic procedures to determine the aetiology of the solitary pulmonary nodules (SPN) identified. We have developed a novel procedure which allows sampling of SPNs via a transparenchymal approach, and report the results from this as a first in human trial. METHODS: This study was a prospective single-arm interventional study. We recruited patients with a SPN detected on CT imaging, which was suspicious for lung cancer, who were suitable for surgical resection...
April 2015: Thorax
Daniel H Sterman, Thomas Keast, Lav Rai, Jason Gibbs, Henky Wibowo, Jeff Draper, Felix J Herth, Gerard A Silvestri
BACKGROUND: Bronchoscopic transparenchymal nodule access (BTPNA) is a novel approach to accessing pulmonary nodules. This real-time, image-guided approach was evaluated for safety, accuracy, and yield in the healthy canine model. METHODS: A novel, inorganic model of subcentimeter pulmonary nodules was developed, consisting of 0.25-cc aliquots of calcium hydroxylapatite (Radiesse) implanted via transbronchial access in airways seven generations beyond the main bronchi to represent targets for evaluation of accuracy and yield...
March 2015: Chest
Dibyendu Paik, Partha Das, Tripti De, Tapati Chakraborti
Leishmaniasis, a neglected tropical disease (NTD) causes major health problems in the tropical and subtropical world. Most of the antileishmanial modern therapies with different formulations of pentavalent antimonials, Miltefosine, Amphotericin B etc. are not satisfactory in recent times due to high toxicity to the host and present rising strain resistance issues. So there is an urgent need to develop new, safe and cost-effective drugs against leishmaniasis. In this regard, bioactive phytocomponents may lead to the discovery of new medicines with appropriate efficiency...
November 2014: Experimental Parasitology
Gerard A Silvestri, Felix J Herth, Thomas Keast, Lav Rai, Jason Gibbs, Henky Wibowo, Daniel H Sterman
BACKGROUND: The current approaches for tissue diagnosis of a solitary pulmonary nodule are transthoracic needle aspiration, guided bronchoscopy, or surgical resection. The choice of procedure is driven by patient and radiographic factors, risks, and benefits. We describe a new approach to the diagnosis of a solitary pulmonary nodule, namely bronchoscopic transparenchymal nodule access (BTPNA). METHODS: In anesthetized dogs, fiducial markers were placed and thoracic CT images acquired...
April 2014: Chest
Caterina Temporini, Enrica Calleri, Daniel Campèse, Karin Cabrera, Guy Félix, Gabriella Massolini
The preparation and optimization of a new monolithic chymotrypsin bioreactor for online protein digestion is described. Silica monolithic supports have been activated with epoxide functionalities following an optimized in situ procedure and used for covalent immobilization of chymotrypsin in one-step reaction under different conditions. A total of four bioreactors were prepared and characterized in terms of the amount of immobilized enzyme and apparent active units by using a standard substrate, N-benzoyl-L-tyrosine p-nitroanilide (BTPNA)...
November 2007: Journal of Separation Science
Saleh A Mohamed, Afaf S Fahmy, Tarek M Mohamed, Soha M Hamdy
Proteolytic activity of 0-12 day old eggs, miracidium and adult worm of Fasciola gigantica was assessed and proteases were partially purified by DEAE-Sepharose and CM-cellulose columns. Four forms of protease were separated, PIa, PIb, PIc and PII. Purifications were completed for PIc and PII using Sephacryl S-200 chromatography. A number of natural and synthetic proteins were tested as substrates for F. gigantica PIc and PII. The two proteases had moderate activity levels toward azoalbumin and casein compared to azocasein, while gelatin, hemoglobin, albumin and fibrin had very low affinity toward the two enzymes...
October 2005: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
A P Valaitis, S Augustin, K M Clancy
Three proteolytic enzymes, trypsin, chymotrypsin, and aminopeptidase-N (APN), were purified from laboratory-reared western spruce budworm, Choristoneura occidentalis [Freeman], larvae. Budworm trypsin exhibited a high degree of substrate specificity, was inactivated by DFP and TLCK, and was inhibited by trypsin inhibitors. The western spruce budworm chymotrypsin hydrolyzed SAAPFpNA and SAAPLpNA, but not SFpNA, SGGFpNA, SGGLpNA or BTpNA. The chymotrypsin was inactivated by DFP, and was inhibited by chymostatin and the chymotrypsin inhibitor, POT-1...
May 1999: Insect Biochemistry and Molecular Biology
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