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https://www.readbyqxmd.com/read/29778714/continuous-mononuclear-cell-collection-cmnc-protocol-impact-on-hematopoietic-stem-cell-collections-in-donors-with-negative-collection-predictors
#1
Krzysztof Bartnik, Martyna Maciejewska, Roiya Farhan, Elżbieta Urbanowska, Małgorzata Król, Maria Król, Magdalena Feliksbrot, Wiesław Wiktor-Jędrzejczak, Emilian Snarski
BACKGROUND: Recently, novel protocol utilizing Continuous Mononuclear Cell Collection (cMNC) have been introduced for leukapheresis. We compared the efficacy of cMNC with an older protocol - mononuclear cell collection (MNC) for CD34+ cell collection in unrelated donors with negative stem cell collection predictors. MATERIAL AND METHODS: Retrospective data from a series of 258 consecutive unrelated hematopoietic stem cell donors was included in this single-center study (80 donors collected with cMNC and 178 with MNC)...
April 22, 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29767061/biochemical-and-histopathological-changes-in-sheep-fed-different-detoxified-karanj-pongamia-glabra-seed-cake-as-partial-protein-supplements
#2
Nira M Soren, Anil K Sharma, Vadali R B Sastry
The study investigated the long-term effect of feeding processed solvent extracted karanj ( Pongamia glabra ) cake (SKC) on gross pathology and histopathological changes in some vital organs, and on the activities of serum enzymes in Jalauni lambs. Twenty-four male lambs were divided into 4 groups and allotted randomly to a soybean meal (SBM) based control (CON) and 3 treatment groups receiving concentrate mixtures, containing water washed (WW), 2.5% lime (LM) and 0.4% binder (BN) treated SKC replacing 50% nitrogen of SBM to meet the protein requirements...
June 2017: Animal nutrition
https://www.readbyqxmd.com/read/29732573/evaluation-of-the-new-continuous-mononuclear-cell-collection-protocol-versus-an-older-version-on-two-different-apheresis-machines
#3
Silvia Spoerl, Dagmar Wäscher, Stefanie Nagel, Christian Peschel, Mareike Verbeek, Katharina Götze, Angela M Krackhardt
BACKGROUND: Cell separators are routinely used to collect CD34+ blood stem cells in the context of customized stem cell transplantation procedures. The Spectra Optia (Terumo BCT) is a novel development of the precursor instrument, the Cobe Spectra (Terumo BCT). STUDY DESIGN AND METHODS: In this report, 146 autologous and 42 allogeneic donors undergoing apheresis on the Cobe Spectra using the mononuclear cell (MNC) program 4.7 or on the Spectra Optia using the new continuous mononuclear cell (cMNC) program 11...
May 6, 2018: Transfusion
https://www.readbyqxmd.com/read/29538638/natural-conception-rates-in-couples-with-unexplained-or-mild-male-subfertility-scheduled-for-fertility-treatment-a-secondary-analysis-of-a-randomized-controlled-trial
#4
R van Eekelen, R I Tjon-Kon-Fat, P M M Bossuyt, N van Geloven, M J C Eijkemans, A J Bensdorp, F van der Veen, B W Mol, M van Wely
STUDY QUESTION: What is the natural conception rate over the course of 12 months in couples with unexplained or mild male subfertility who are scheduled for fertility treatment and have a predicted unfavourable prognosis for natural conception? SUMMARY ANSWER: The natural conception rate over the course of 12 months in couples who were allocated to treatment was estimated to be 24.5% (95% CI: 20-29%). WHAT IS KNOWN ALREADY: After starting treatment, couples often perceive unsuccessful cycles as evidence of definitive failure even though they are still able to conceive naturally in between and after treatment...
May 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29465755/comparison-of-the-cmnc-and-mnc-apheresis-protocol-for-the-collection-of-t-cells-showed-comparable-outcome-an-observational-study-in-a-single-centre
#5
Daniel Putensen, Richard Smith, Linda Pilcher, George Trandafir
BACKGROUND: An increasing demand for human lymphocytes require an efficient, reliable and reproducible lymphocyte process. Here, we compare the Spectra Optia® CMNC protocol with the Optia MNC platform in unmobilised donor lymphocyte collections. PURPOSE: To establish and compare the feasibility, efficiency, and practicability of the two apheresis protocols. METHODS: Data was collected prospectively from 60 non-cytokine stimulate donors who underwent a total of 64 T-cell collection procedures...
February 21, 2018: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/29451308/apheresis-buffy-coat-collection-without-photoactivation-has-no-effect-on-apoptosis-cell-proliferation-and-total-viability-of-mononuclear-cells-collected-using-photopheresis-systems
#6
Zbigniew M Szczepiorkowski, Christine A Burnett, Larry J Dumont, Sunil H Abhyankar
BACKGROUND: Extracorporeal photopheresis (ECP) has been approved for the treatment of advanced cutaneous T-cell lymphoma since 1988. While the precise mechanisms resulting in clinical effects are not fully understood, the photoactivation of mononuclear cells (MNCs) using ultraviolet A (UVA) light and methoxsalen is believed to be the predominant initiating process. The effects of MNC passage through the instrument without photoactivation are unknown. The objective of this study was to evaluate the effect of cell processing through the photopheresis instruments on MNCs...
April 2018: Transfusion
https://www.readbyqxmd.com/read/29210068/optimal-storage-conditions-for-apheresis-research-oscar-a-biomedical-excellence-for-safer-transfusion-best-collaborative-study
#7
Thomas J Gniadek, Henk S P Garritsen, David Stroncek, Zbigniew M Szczepiorkowski, David H McKenna
BACKGROUND: Cell therapy products are often stored and transported between sites. The aim of this study was to determine the effect of storage temperature, solution, and cell concentration on nonmobilized, peripheral blood-derived mononuclear cells (MNCs). STUDY DESIGN AND METHODS: This was a multicenter prospective study involving healthy volunteers who underwent nonmobilized MNC collection by apheresis. Products were processed at local laboratories and concentrated to either 100 × 106 or 300 × 106 nucleated cells/mL in 5% human serum albumin (HSA) or HypoThermosol FRS (HT; BioLife Solutions)...
February 2018: Transfusion
https://www.readbyqxmd.com/read/29070145/-effect-of-erk1-2-signaling-pathway-inhibitor-pd98059-on-the-expression-of-ras-braf-mek-erk1-2-in-marrow-nucleated-red-blood-cells-of-cms-patients
#8
Yuan-Fang Han, Lin-Hua Ji, Ting-Ting Feng, Fang Liu, Sen Cui, Juan Su
OBJECTIVE: To investigate the effect of ERK1 / 2 signaling pathway inhibitor PD98059 on Ras, Raf, MEK, ERK1, ERK2 expression in order to explore a new way for basic research and clinical treatment of the chronic mountain sickness(CMS). METHODS: Sixteen CMS patients were selected, the bone marrow was collected for isolation of monomuclear cells (MNC), the cells were sorted by using CD71 and CD235a antibody magnetic beads, then positive cells were diveded into 5 groups: blank control, DMSO and PD98059 5, 10 and 20 µmol/L, and were cultured in hypoxid condition for 72 hours...
October 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28954978/stroke-and-the-cell-therapy-saga-towards-a-safe-swift-and-efficient-utilization-of-cells
#9
Nathalie Kubis
The first clinical trials of cell therapy in stroke were first published in the 2000s and consisted of neural stems cells transplanted via the intracerebral pathway. Since mesenchymal stem cells showed similar capacities to differentiate into neural cells and allowed autologous cell transplantation, they were then preferentially studied, including diabetes and hypertension. More recently, bone marrow derived mononuclear cells were successfully transplanted in stroke with no need of culture processing, and simple collection by density gradient centrifugation rendering them immediately ready for use...
2017: Keio Journal of Medicine
https://www.readbyqxmd.com/read/28947358/rice-protein-prolamin-promotes-anti-leukemia-immunity-and-inhibits-leukemia-growth-in-vivo
#10
Chien-Kuo Liu, Chien-An Chen, Tzung-Yan Lee, Hen-Hong Chang, Hui-Fen Liao, Yu-Jen Chen
Prolamin is a heat-stable storage protein of rice (Oryza sativa). This study aimed to examine the effect of prolamin on anti-tumor immune response in vitro and leukemia growth in vivo. The prolamin-enriched rice fractions were prepared to stimulate peripheral blood mononuclear cells (MNC) from mice spleen. The MNC-conditioned medium (MNC-CM) was collected to treat leukemia L1210 cells. Human MNC-CM was prepared to treat Jurkat acute T cell leukemia cells. Purified prolamin was orally administered to syngeneic L1210-bearing DBA/2 mice to assess weights of tumor, liver and spleen, liver histopathology, peripheral blood neutrophil count and cytokine levels...
February 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28940295/comparing-leukapheresis-protocols-for-an-aml-patient-with-symptomatic-leukostasis
#11
Abigail Cline, Ryan Jajosky, James Shikle, Roni Bollag
BACKGROUND: Acute myeloid leukemia (AML) is a malignancy characterized by rapid clonal proliferation of myeloid precursors, which can result in hyperleukocytosis. Leukapheresis can be used to rapidly reduce the white blood cell count (WBC). However, the only FDA cleared device for WBC depletion, the COBE Spectra, will no longer be supported by the manufacturer in 2017, and there are few studies comparing different methods of leukapheresis. CASE REPORT: A 68-year-old African American female was admitted to the hospital for relapse of her AML...
September 22, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/28809049/evolution-of-mnc-and-lymphocyte-collection-settings-employing-different-spectra-optia-%C3%A2-leukapheresis-systems
#12
COMPARATIVE STUDY
M Punzel, A Kozlova, A Quade, A H Schmidt, R Smith
BACKGROUND AND OBJECTIVES: The Spectra Optia® continuous mononuclear cell (CMNC apheresis) system has emerged as the preferred device in peripheral blood stem cell collections over the original two-step Spectra Optia® mononuclear cell (MNC apheresis) system. Until now, no comparative data were available for non-stimulated MNC collections that are required for immunotherapy. MATERIALS AND METHODS: We compared collection parameters and product composition for Spectra Optia MNC- as well as CMNC-apheresis systems in non-stimulated MNC collections from 35 registry donors intended for donor lymphocyte infusions...
August 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28800844/first-comparative-analysis-concerning-the-plasma-platelet-contamination-during-mnc-collection
#13
Hella Pfeiffer, Susanne Achenbach, Julian Strobel, Robert Zimmermann, Reinhold Eckstein, Erwin F Strasser
BACKGROUND AND OBJECTIVES: Monocytes can be cultured into dendritic cells with addition of autologous plasma, which is highly prone to platelet contamination due to the apheresis process. Since platelets affect the maturation process of monocytes into dendritic cells and might even lead to a diminished harvest of dendritic cells, it is very important to reduce the platelet contamination. A new collection device (Spectra Optia) was analyzed, compared to two established devices (COM.TEC, Cobe Spectra) and evaluated regarding the potential generation of source plasma...
July 13, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28731276/attempt-to-harvest-a-sufficient-number-of-mononuclear-cells-in-an-appropriate-blood-product-volume-by-modification-of-the-default-apheresis-setting
#14
Yumi Tanaka, Kohshi Ohishi, Toshiki Sawai, Hitoshi Iwasaki, Shinichi Kageyama, Masahiro Masuya, Takeshi Matsumoto, Takashi Tanigawa, Hideo Wada, Hiroshi Shiku, Masaaki Ito, Naoyuki Katayama
To harvest for T cell therapy, a 1.6-fold higher number of CD3(+) T cells was collected with MNC mode (N = 10) compared with Auto PBSC mode (N = 5) in COBE Spectra cell separator, but the blood product volume was increased by 3.5-fold. For therapeutic angiogenesis therapy, apheresis was initially performed using Auto PBSC mode (N = 4) to fine tune the blood product volume to omit cell concentration, but the collected number of mononuclear cells was lower than expected. However, an increase of the harvest cycle number from 3...
October 2017: Therapeutic Apheresis and Dialysis
https://www.readbyqxmd.com/read/28603176/autologous-bone-marrow-mononuclear-cell-implantation-and-its-impact-on-the-outcome-of-patients-with-critical-limb-ischemia%C3%A3-results-of-a-randomized-double-blind-placebo-controlled-trial
#15
Bernard Pignon, Marie-Antoinette Sevestre, Lukshe Kanagaratnam, Gilles Pernod, Dominique Stephan, Joseph Emmerich, Claude Clement, Gabrielle Sarlon, Carine Boulon, Claire Tournois, Philippe Nguyen
BACKGROUND: Cell therapy is a therapeutic option for patients presenting with nonrevascularizable critical limb ischemia (CLI). However there is a lack of firm evidence on its efficacy because of the paucity of randomized controlled trials.Methods and Results:The BALI trial was a multicenter, randomized, controlled, double-blind clinical trial that included 38 patients. For all of them, 500 mL of bone marrow were collected for preparation of a BM-MNC product that was implanted in patients assigned to active treatment...
June 10, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28485045/performance-and-safety-of-femoral-central-venous-catheters-in-pediatric-autologous-peripheral-blood-stem-cell-collection
#16
Laura Cooling, Sandra Hoffmann, Dawn Webb, Chisa Yamada, Robertson Davenport, Sung Won Choi
INTRODUCTION: Autologous peripheral blood hematopoietic progenitor cell collection (A-HPCC) in children typically requires placement of a central venous catheter (CVC) for venous access. There is scant published data regarding the performance and safety of femoral CVCs in pediatric A-HPCC. METHODS: Seven-year, retrospective study of A-HPCC in pediatric patients collected between 2009 and January 2017. Inclusion criteria were an age ≤ 21 years and A-HPCC using a femoral CVC for venous access...
December 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/28445013/comparison-of-two-apheresis-systems-of-cobe-and-optia-for-autologous-peripheral-blood-stem-cell-collection
#17
COMPARATIVE STUDY
Se Na Lee, Ji Yeon Sohn, Jung Hee Kong, Hyeon Seok Eom, Hyewon Lee, Sun Young Kong
Peripheral blood stem cell (PBSC) transplantation following myeloablative therapy is a mainstay of treatment for various types of malignancies. This study aimed to evaluate the differences between the Optia MNC and COBE Spectra MNC systems (Terumo BCT, Japan) according to apheresis procedures and the parameters of apheresis, products, and collection. The clinical data of 74 patients who underwent autologous PBSC collection from July 2012 to July 2015 were reviewed retrospectively. The patients comprised 48 (65%) men and 26 (35%) women with a median age of 56 yr (range, 23-66 yr)...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28357515/clinical-features-and-predictors-of-patients-with-critical-limb-ischemia-who-responded-to-autologous-mononuclear-cell-transplantation-for-therapeutic-angiogenesis
#18
Naoyoshi Aoyama, Makoto Nishinari, Shinichi Ohtani, Akifumi Kanai, Chiharu Noda, Mitsuhiro Hirata, Akira Miyamoto, Masafumi Watanabe, Tohru Minamino, Tohru Izumi, Jyunya Ako
The clinical features of patients with critical limb ischemia (CLI) who responded to angiogenesis using autologous peripheral blood mononuclear cell transplantation (PB-MNC) have not yet been fully characterized, and there are no useful predictors to judge the curative effect in the early period after PB-MNC. This study sought to clarify the clinical features and predictors in patients with CLI who were successfully treated using PB-MNC. 30 consecutive patients [arteriosclerosis obliterans: 24 patients, thromboangiitis obliterans: 6 patients] who were diagnosed with major amputation despite maximal medical therapy were enrolled in this study...
September 2017: Heart and Vessels
https://www.readbyqxmd.com/read/28340039/infant-birth-outcomes-are-associated-with-dna-damage-biomarkers-as-measured-by-the-cytokinesis-block-micronucleus-cytome-assay-the-dadhi-study
#19
Mansi Dass Singh, Philip Thomas, Maryam Hor, Theodora Almond, Julie Owens, William Hague, Michael Fenech
Accumulation of DNA damage in the first 1000 days may increase risk of accelerated ageing and degenerative diseases in adult life such as cancers. The extent of DNA damage in infants and the correlation of maternal factors during pregnancy with neonate birth outcomes and DNA damage is not known in infants born in Australia. Therefore, we performed a prospective cohort study to collect data on DNA damage in lymphocytes of Australian infants (aged 0, 3 and 6 months), using the cytokinesis block micronucleus cytome (CBMN-Cyt) assay...
May 1, 2017: Mutagenesis
https://www.readbyqxmd.com/read/28320105/anti-fibrotic-potential-of-human-umbilical-cord-mononuclear-cells-and-mouse-bone-marrow-cells-in-ccl-4-induced-liver-fibrosis-in-mice
#20
Nageh Ahmed Elmahdy, Samia Salem Sokar, Mohamed Labib Salem, Naglaa Ibrahim Sarhan, Sherin Hamed Abou-Elela
Liver fibrosis is the consequence of hepatocyte injury that leads to the activation of hepatic stellate cells (HSC). The treatment of choice is Liver transplantation; however, it has many problems such as surgery-related complications, immunological rejection and high costs associated with the procedure. Stem cell-based therapy would be a potential alternative, so the aim of this study is to investigate the therapeutic potential of human umbilical cord mononuclear cells (MNC) and mouse bone marrow cells (BMC) against carbon tetrachloride (CCl4 ) induced liver fibrosis in mice and compare it with that of silymarin...
May 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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