keyword
MENU ▼
Read by QxMD icon Read
search

mnc collection

keyword
https://www.readbyqxmd.com/read/28320105/anti-fibrotic-potential-of-human-umbilical-cord-mononuclear-cells-and-mouse-bone-marrow-cells-in-ccl4-induced-liver-fibrosis-in-mice
#1
Nageh Ahmed Elmahdy, Samia Salem Sokar, Mohamed Labib Salem, Naglaa Ibrahim Sarhan, Sherin Hamed Abou-Elela
Liver fibrosis is the consequence of hepatocyte injury that leads to the activation of hepatic stellate cells (HSC). The treatment of choice is Liver transplantation; however, it has many problems such as surgery-related complications, immunological rejection and high costs associated with the procedure. Stem cell-based therapy would be a potential alternative, so the aim of this study is to investigate the therapeutic potential of human umbilical cord mononuclear cells (MNC) and mouse bone marrow cells (BMC) against carbon tetrachloride (CCl4) induced liver fibrosis in mice and compare it with that of silymarin...
March 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28167301/non-invasive-tracking-of-injected-bone-marrow-mononuclear-cells-to-injury-and-implanted-biomaterials
#2
Richard P Tan, Bob S L Lee, Alex H P Chan, Sui Ching G Yuen, Juichien Hung, Steven G Wise, Martin K C Ng
Biomaterial scaffolds enhancing the engraftment of transplanted bone-marrow mononuclear cells (BM-MNC) have enormous potential for tissue regeneration applications. However, development of appropriate materials is challenging given the precise microenvironments required to support BM-MNC engraftment and function. In this study, we have developed a non-invasive, real-time tracking model of injected BM-MNC engraftment to wounds and implanted biomaterial scaffolds. BM-MNCs, encoded with firefly luciferase and enhanced GFP reporter genes, were tail vein injected into subcutaneously wounded mice...
February 3, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28150319/factors-influencing-platelet-clumping-during-peripheral-blood-hematopoietic-stem-cell-collection
#3
Gagan Mathur, Sarah L Mott, Laura Collins, Gail A Nelson, C Michael Knudson, Annette J Schlueter
BACKGROUND: Platelet clumping is a common occurrence during peripheral blood hematopoietic stem cell (HSC) collection using the Spectra Optia mononuclear cell (MNC) protocol. If clumping persists, it may prevent continuation of the collection and interfere with proper MNC separation. This study is the first to report the incidence of clumping, identify precollection factors associated with platelet clumping, and describe the degree to which platelet clumping interferes with HSC product yield...
February 1, 2017: Transfusion
https://www.readbyqxmd.com/read/28118760/road-safety-perspectives-among-employees-of-a-multinational-corporation-in-urban-india-local-context-for-global-injury-prevention
#4
Sara F Jacoby, Flaura K Winston, Therese S Richmond
In rapidly developing economies, like urban India, where road traffic injury rates are among the world's highest, the corporate workplace offers a non-traditional venue for road safety interventions. In partnership with a major multinational corporation (MNC) with a large Indian workforce, this study aimed to elicit local employee perspectives on road safety to inform a global corporate health platform. The safety attitudes and behaviours of 75 employees were collected through self-report survey and focus groups in the MNC offices in Bangalore and Pune...
January 24, 2017: International Journal of Injury Control and Safety Promotion
https://www.readbyqxmd.com/read/28024510/-effect-of-different-hemapheresis-procedures-on-components-of-hematopoietic-stem-cells-collected-from-healthy-donors
#5
Chun-Hong Sun, Jian-Na Yao, Fang Liu, Xiao-Juan Wang, Juan-Juan Ma, Mi-Mi Cui, Mei Zhang, Xiao-Ning Wang
OBJECTIVE: To investigate the effects of different hemapheresis procedures on the components of hematopoietic stem cells(HSCs) collected from helathy donors. METHODS: twelve donors were underwent stem cell collection from January 2015 to August 2016, and the stem cells were randomly colleted by AutoPBSC procedure of COBE spectra and MNC procedure of the Spectra Optia blood cell separator, the mononuclear cells, CD34(+) cells, granulocytes, lymphocytes and platelets in the collections were compared...
December 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28019012/improved-planning-of-leukapheresis-endpoint-with-customized-prediction-algorithm-minimizing-collection-days-volume-of-blood-processed-procedure-time-and-citrate-toxicity
#6
Daniel L Leberfinger, Kira L Badman, Joseph M Roig, Tamara Loos
BACKGROUND: Increased emphasis on cost and productivity in apheresis centers calls for a proficient and predictable hematopoietic progenitor cell (HPC) collection. Therefore, the aim of this study was to determine the value of a customized predictive algorithm that estimates how many blood volumes are required to process for a targeted CD34 cell dose. STUDY DESIGN AND METHODS: Retrospective analysis was performed on 107 HPC collections completed on the Spectra Optia MNC from January 2013 to June 2014 in 51 patients and 12 donors...
March 2017: Transfusion
https://www.readbyqxmd.com/read/27906549/anti-inflammatory-and-ros-suppressive-effects-of-the-addition-of-fiber-to-a-high-fat-high-calorie-meal
#7
Husam Ghanim, Manav Batra, Sanaa Abuaysheh, Kelly Green, Antoine Makdissi, Nitesh Kuhadiya, Ajay Chaudhuri, Paresh Dandona
BACKGROUND: Fiber intake is associated with a reduction in the occurrence of cardiovascular events and diabetes. OBJECTIVE: To investigate whether the addition of fiber to a high fat-high Calorie (HFHC) meal prevents pro-inflammatory changes induced by HFHC meal. DESIGN: Ten fasting normal subjects consumed HFHC meal with or without an additional 30g of insoluble dietary fiber on 2 separate visits. Blood samples were collected over 5hr and mononuclear cells (MNC) isolated...
December 1, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27891769/exenatide-induces-an-increase-in-vasodilatory-and-a-decrease-in-vasoconstrictive-mediators
#8
Ajay Chaudhuri, Husam Ghanim, Antoine Makdissi, Kelly Green, Sanaa Abuaysheh, Manav Batra, Nitesh Kuhadiya, Paresh Dandona
In view of the known vasodilatory effects of GLP-1, and exenatide, we investigated the effects of exenatide on vasoactive factors. We analyzed blood samples and mononuclear cells (MNC) from a previous study collected after a single dose and following 12 weeks of exenatide or placebo treatment on a series of 24 patients with T2DM. Following exenatide, plasma concentrations of ANP, cGMP, cAMP increased significantly at 12 weeks. Plasma cGMP and adenylate cyclase expression in MNC increased significantly following single dose...
November 28, 2016: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/27882430/identification-of-cardiovascular-risk-factors-associated-with-bone-marrow-cell-subsets-in-patients-with-stemi-a-biorepository-evaluation-from-the-cctrn-time-and-latetime-clinical-trials
#9
Ariadna Contreras, Aaron F Orozco, Micheline Resende, Robert C Schutt, Jay H Traverse, Timothy D Henry, Dejian Lai, John P Cooke, Roberto Bolli, Michelle L Cohen, Lem Moyé, Carl J Pepine, Phillip C Yang, Emerson C Perin, James T Willerson, Doris A Taylor
Autologous bone marrow mononuclear cell (BM-MNC) therapy for patients with ST-segment elevation myocardial infarction (STEMI) has produced inconsistent results, possibly due to BM-MNC product heterogeneity. Patient-specific cardiovascular risk factors (CRFs) may contribute to variations in BM-MNC composition. We sought to identify associations between BM-MNC subset frequencies and specific CRFs in STEMI patients. Bone marrow was collected from 191 STEMI patients enrolled in the CCTRN TIME and LateTIME trials...
January 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/27784387/-effect-of-different-procedures-from-blood-cell-separators-on-autologous-pbsc-collection-efficiency
#10
Xiao-Ning Wang, Jian-Na Yao, Fang Liu, Xiao-Juan Wang, Juan-Juan Ma, Cai-Li Guo, Hua-Sheng Liu, Peng-Cheng He, Mei Zhang, Chun-Hong Sun
OBJECTIVE: To observe the efficacy and adverse reactions of autologous PBSC collection when the autoPBSC procedure and MNC procedure of COBE Spectra cell separator and the MNC procedure of Spectra Optia cell separator were used. METHODS: The autologous perepheral blood hematopoietic stem cells from 41 patients were collected by using autoPBSC procedure and MNC procedure of COBE Spectra blood cell separator and MNC procedure of Spectra Optia blood cell separator...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27784364/-effects-of-hepatovirus-b-infection-on-the-hematopoietic-stem-cell-collection-and-implantment-in-lymphoma-patients-received-autologous-peripheral-blood-hematopoietic-stem-cell-transplantation
#11
Xiao-Ning Wang, Peng-Cheng He, Cai-Li Guo, Chun-Hong Sun, Mei Zhang, Hua-Sheng Liu
OBJECTIVE: To investigate the effect of hepatovirus B(HBV) infection on the hematopoietic stem cell collection and implantment in lymphoma patients received autologous peripheral hematopoietic blood stem cells transplantation. METHODS: Clinical data of 40 lymphoma patients who received autologous peripheral hematopoietic blood stem cell transplantation between January 2006 and October 2014 was analyzed retrospectively. Among 40 patients with lymphoma 8 patients combined with HBV infection were prophylacticly given nucleoside analogues and 32 patients without HBV infection...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27652247/assessment-of-micronuclei-frequency-in-individuals-with-a-habit-of-tobacco-by-means-of-exfoliated-oral-buccal-cells
#12
Tanvi Dosi, Dhaman Gupta, Alka Hazari, Rajan Rajput, Prabhav Chauhan, Anushri S Rajapuri
AIMS AND OBJECTIVES: To study the genotoxic effects of tobacco on the exfoliated buccal epithelial cells in patients with oral precancerous lesions (OPLs) and Patients with tobacco habit but without oral precancerous lesion(habit controls) by using micronucleus assay as well as the quantification and detection of the biomarkers in these premalignant lesions which will be helpful in finding those patients who are at higher risk for malignant transformation. MATERIALS AND METHODS: Forty samples were collected from the right and left side of buccal epithelial cells obtained from 20 individuals, i...
August 2016: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/27589225/continuous-cd34-cell-collection-by-a-new-device-is-safe-and-more-efficient-than-by-a-standard-collection-procedure-results-of-a-two-center-crossover-randomized-trial
#13
Jose A Cancelas, Edward P Scott, Jerome R Bill
BACKGROUND: Peripheral blood stem cells (PBSCs) collected from granulocyte-colony-stimulating factor (G-CSF)-mobilized donors are routinely used for hematopoietic stem cell transplantation. Most PBSC collections worldwide have used the COBE Spectra (COBE) platform that is being replaced by the Spectra Optia (OPTIA). This study compared the PBSC collection performance and safety of the OPTIA using a single-step, continuous mononuclear cell (CMNC) collection procedure and the standard COBE MNC procedure...
September 2, 2016: Transfusion
https://www.readbyqxmd.com/read/27452644/multiple-small-versus-few-large-amount-aspirations-for-bone-marrow-harvesting-in-autologous-and-allogeneic-bone-marrow-transplantation
#14
Volker Witt, Herbert Pichler, Gerhard Fritsch, Christina Peters
For successful bone marrow transplantation it is necessary to obtain enough progenitor cells during the bone marrow (BM) harvesting procedure. Most centers are using multiple aspirations of maximum 2 ml BM (A), while other centers are using few larger amount aspirations for BM harvesting (B). There is still a discussion about possible differences in graft composition between A and B. To evaluate the feasibility in children we evaluated twenty BM harvestings that were performed in 18 donors, 7 autologous (median age 6...
October 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/27442837/peripheral-blood-stem-cells-collection-on-spectra-optia-apheresis-system-using-the-continuous-mononuclear-cell-collection-protocol-a-single-center-report-of-39-procedures
#15
F Sanderson, P Poullin, R Smith, C Nicolino-Brunet, P Philip, A Chaib, R Costello
BACKGROUND: The aim of the study was to assess the performance of the new Continuous Mononuclear Cell Collection (CMNC) protocol on the Spectra Optia Apheresis System for collecting autologous Peripheral Blood Stem Cells (PBSC) in adult patients with respect to collection variables, CD34+ cells harvest prediction and engraftment data. MATERIALS AND METHODS: In this retrospective study, 39 CMNC procedures on 23 mobilized patients with multiple myeloma and lymphoma were analyzed...
July 21, 2016: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/27387375/histochemical-study-of-the-effects-on-abomasal-mucins-of-haemonchus-contortus-or-teladorsagia-circumcincta-infection-in-lambs
#16
H V Simpson, S Umair, V C Hoang, M S Savoian
Previously, chemical analysis of gastric fundic mucin showed that infection of sheep with Haemonchus contortus or Teladorsagia circumcincta changed the proportions of monosaccharides and decreased terminal mucin fucosylation and sialylation. To identify the effects of these parasites on the two mucin-secreting cell lineages, fundic and antral tissues were collected for histochemistry from 69 lambs aged from 3-4 to 9-10 months-of-age which had received a single infection of either H. contortus or T. circumcincta and euthanased at Day 21 or 28 post- infection respectively...
August 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27301272/lactobacillus-rhamnosus-gg-modulates-innate-signaling-pathway-and-cytokine-responses-to-rotavirus-vaccine-in-intestinal-mononuclear-cells-of-gnotobiotic-pigs-transplanted-with-human-gut-microbiota
#17
Haifeng Wang, Kan Gao, Ke Wen, Irving Coy Allen, Guohua Li, Wenming Zhang, Jacob Kocher, Xingdong Yang, Ernawati Giri-Rachman, Guan-Hong Li, Sherrie Clark-Deener, Lijuan Yuan
BACKGROUND: A better understanding of mechanisms underlying dose-effects of probiotics in their applications as treatments of intestinal infectious or inflammatory diseases and as vaccine adjuvant is needed. In this study, we evaluated the modulatory effects of Lactobacillus rhamnosus GG (LGG) on transplanted human gut microbiota (HGM) and on small intestinal immune cell signaling pathways in gnotobiotic pigs vaccinated with an oral attenuated human rotavirus (AttHRV) vaccine. RESULTS: Neonatal HGM transplanted pigs were given two doses of AttHRV on 5 and 15 days of age and were divided into three groups: none-LGG (AttHRV), 9-doses LGG (AttHRV + LGG9X), and 14-doses LGG (AttHRV + LGG14X) (n = 3-4)...
2016: BMC Microbiology
https://www.readbyqxmd.com/read/27292585/effect-of-testosterone-on-hepcidin-ferroportin-ferritin-and-iron-binding-capacity-in-patients-with-hypogonadotropic-hypogonadism-and-type-2-diabetes
#18
Sandeep Dhindsa, Husam Ghanim, Manav Batra, Nitesh D Kuhadiya, Sanaa Abuaysheh, Kelly Green, Antoine Makdissi, Ajay Chaudhuri, Paresh Dandona
CONTEXT: As the syndrome of hypogonadotropic hypogonadism (HH) is associated with anaemia and the administration of testosterone restores haematocrit to normal, we investigated the potential underlying mechanisms. DESIGN: Randomized, double-blind, placebo-controlled trial. METHODS: We measured basal serum concentrations of erythropoietin, iron, iron binding capacity, transferrin (saturated and unsaturated), ferritin and hepcidin and the expression of ferroportin and transferrin receptor (TR) in peripheral blood mononuclear cells (MNC) of 94 men with type 2 diabetes...
November 2016: Clinical Endocrinology
https://www.readbyqxmd.com/read/27250563/comparison-of-two-automated-mononuclear-cell-collection-systems-in-patients-undergoing-extracorporeal-photopheresis-a-prospective-crossover-equivalence-study
#19
Claudia Del Fante, Luigia Scudeller, Alberto Martinasso, Gianluca Viarengo, Cesare Perotti
BACKGROUND: Extracorporeal photopheresis (ECP) is an effective cell therapy employed in several diseases, including graft versus host disease (GVHD) and organ rejection. When ECP is performed using an off-line technique, mononuclear cell (MNC) collection by leukapheresis is necessary for further manipulation (addition of 8-methoxypsoralen and ultraviolet A irradiation before reinfusion to the patient). We report the results of the first crossover equivalence study on yield and purity of MNCs collected from patients undergoing ECP with two different automated systems: MNC and CMNC (working with intermittent and continuous-flow collection, respectively), released by Terumo BCT...
August 2016: Transfusion
https://www.readbyqxmd.com/read/27236676/isolation-culture-and-characterization-of-human-umbilical-cord-blood-derived-mesenchymal-stromal-cells
#20
Karen Bieback, Philipp Netsch
Umbilical cord blood (CB) is considered one of the youngest available sources of adult stem cells. Besides hematopoietic stem cells, CB has been shown to contain endothelial progenitor cells as well as mesenchymal stromal/stem cells (MSC). To isolate MSC from cord blood, CB is collected into a sterile bag containing the anticoagulant citrate-phosphate-dextrose (CPD). The CB is then processed by density-gradient centrifugation to obtain mononuclear cells (MNC). These are cultured until the outgrowth of fibroblastoid cell colonies appears...
2016: Methods in Molecular Biology
keyword
keyword
56445
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"