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https://www.readbyqxmd.com/read/27906549/anti-inflammatory-and-ros-suppressive-effects-of-the-addition-of-fiber-to-a-high-fat-high-calorie-meal
#1
Husam Ghanim, Manav Batra, Sanaa Abuaysheh, Kelly Green, Antoine Makdissi, Nitesh Kuhadiya, Ajay Chaudhuri, Paresh Dandona
BACKGROUND: Fiber intake is associated with a reduction in the occurrence of cardiovascular events and diabetes. OBJECTIVE: To investigate whether the addition of fiber to a high fat-high Calorie (HFHC) meal prevents pro-inflammatory changes induced by HFHC meal. DESIGN: Ten fasting normal subjects consumed HFHC meal with or without an additional 30g of insoluble dietary fiber on 2 separate visits. Blood samples were collected over 5hr and mononuclear cells (MNC) isolated...
December 1, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27891769/exenatide-induces-an-increase-in-vasodilatory-and-a-decrease-in-vasoconstrictive-mediators
#2
Ajay Chaudhuri, Husam Ghanim, Antoine Makdissi, Kelly Green, Sanaa Abuaysheh, Manav Batra, Nitesh Kuhadiya, Paresh Dandona
In view of the known vasodilatory effects of GLP-1, and exenatide, we investigated the effects of exenatide on vasoactive factors. We analyzed blood samples and mononuclear cells (MNC) from a previous study collected after a single dose and following 12 weeks of exenatide or placebo treatment on a series of 24 patients with T2DM. Following exenatide, plasma concentrations of ANP, cGMP, cAMP increased significantly at 12 weeks. Plasma cGMP and adenylate cyclase expression in MNC increased significantly following single dose...
November 28, 2016: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/27882430/identification-of-cardiovascular-risk-factors-associated-with-bone-marrow-cell-subsets-in-patients-with-stemi-a-biorepository-evaluation-from-the-cctrn-time-and-latetime-clinical-trials
#3
Ariadna Contreras, Aaron F Orozco, Micheline Resende, Robert C Schutt, Jay H Traverse, Timothy D Henry, Dejian Lai, John P Cooke, Roberto Bolli, Michelle L Cohen, Lem Moyé, Carl J Pepine, Phillip C Yang, Emerson C Perin, James T Willerson, Doris A Taylor
Autologous bone marrow mononuclear cell (BM-MNC) therapy for patients with ST-segment elevation myocardial infarction (STEMI) has produced inconsistent results, possibly due to BM-MNC product heterogeneity. Patient-specific cardiovascular risk factors (CRFs) may contribute to variations in BM-MNC composition. We sought to identify associations between BM-MNC subset frequencies and specific CRFs in STEMI patients. Bone marrow was collected from 191 STEMI patients enrolled in the CCTRN TIME and LateTIME trials...
January 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/27784387/-effect-of-different-procedures-from-blood-cell-separators-on-autologous-pbsc-collection-efficiency
#4
Xiao-Ning Wang, Jian-Na Yao, Fang Liu, Xiao-Juan Wang, Juan-Juan Ma, Cai-Li Guo, Hua-Sheng Liu, Peng-Cheng He, Mei Zhang, Chun-Hong Sun
OBJECTIVE: To observe the efficacy and adverse reactions of autologous PBSC collection when the autoPBSC procedure and MNC procedure of COBE Spectra cell separator and the MNC procedure of Spectra Optia cell separator were used. METHODS: The autologous perepheral blood hematopoietic stem cells from 41 patients were collected by using autoPBSC procedure and MNC procedure of COBE Spectra blood cell separator and MNC procedure of Spectra Optia blood cell separator...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27784364/-effects-of-hepatovirus-b-infection-on-the-hematopoietic-stem-cell-collection-and-implantment-in-lymphoma-patients-received-autologous-peripheral-blood-hematopoietic-stem-cell-transplantation
#5
Xiao-Ning Wang, Peng-Cheng He, Cai-Li Guo, Chun-Hong Sun, Mei Zhang, Hua-Sheng Liu
OBJECTIVE: To investigate the effect of hepatovirus B(HBV) infection on the hematopoietic stem cell collection and implantment in lymphoma patients received autologous peripheral hematopoietic blood stem cells transplantation. METHODS: Clinical data of 40 lymphoma patients who received autologous peripheral hematopoietic blood stem cell transplantation between January 2006 and October 2014 was analyzed retrospectively. Among 40 patients with lymphoma 8 patients combined with HBV infection were prophylacticly given nucleoside analogues and 32 patients without HBV infection...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27652247/assessment-of-micronuclei-frequency-in-individuals-with-a-habit-of-tobacco-by-means-of-exfoliated-oral-buccal-cells
#6
Tanvi Dosi, Dhaman Gupta, Alka Hazari, Rajan Rajput, Prabhav Chauhan, Anushri S Rajapuri
AIMS AND OBJECTIVES: To study the genotoxic effects of tobacco on the exfoliated buccal epithelial cells in patients with oral precancerous lesions (OPLs) and Patients with tobacco habit but without oral precancerous lesion(habit controls) by using micronucleus assay as well as the quantification and detection of the biomarkers in these premalignant lesions which will be helpful in finding those patients who are at higher risk for malignant transformation. MATERIALS AND METHODS: Forty samples were collected from the right and left side of buccal epithelial cells obtained from 20 individuals, i...
August 2016: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/27589225/continuous-cd34-cell-collection-by-a-new-device-is-safe-and-more-efficient-than-by-a-standard-collection-procedure-results-of-a-two-center-crossover-randomized-trial
#7
Jose A Cancelas, Edward P Scott, Jerome R Bill
BACKGROUND: Peripheral blood stem cells (PBSCs) collected from granulocyte-colony-stimulating factor (G-CSF)-mobilized donors are routinely used for hematopoietic stem cell transplantation. Most PBSC collections worldwide have used the COBE Spectra (COBE) platform that is being replaced by the Spectra Optia (OPTIA). This study compared the PBSC collection performance and safety of the OPTIA using a single-step, continuous mononuclear cell (CMNC) collection procedure and the standard COBE MNC procedure...
September 2, 2016: Transfusion
https://www.readbyqxmd.com/read/27452644/multiple-small-versus-few-large-amount-aspirations-for-bone-marrow-harvesting-in-autologous-and-allogeneic-bone-marrow-transplantation
#8
Volker Witt, Herbert Pichler, Gerhard Fritsch, Christina Peters
For successful bone marrow transplantation it is necessary to obtain enough progenitor cells during the bone marrow (BM) harvesting procedure. Most centers are using multiple aspirations of maximum 2 ml BM (A), while other centers are using few larger amount aspirations for BM harvesting (B). There is still a discussion about possible differences in graft composition between A and B. To evaluate the feasibility in children we evaluated twenty BM harvestings that were performed in 18 donors, 7 autologous (median age 6...
October 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/27442837/peripheral-blood-stem-cells-collection-on-spectra-optia-apheresis-system-using-the-continuous-mononuclear-cell-collection-protocol-a-single-center-report-of-39-procedures
#9
F Sanderson, P Poullin, R Smith, C Nicolino-Brunet, P Philip, A Chaib, R Costello
BACKGROUND: The aim of the study was to assess the performance of the new Continuous Mononuclear Cell Collection (CMNC) protocol on the Spectra Optia Apheresis System for collecting autologous Peripheral Blood Stem Cells (PBSC) in adult patients with respect to collection variables, CD34+ cells harvest prediction and engraftment data. MATERIALS AND METHODS: In this retrospective study, 39 CMNC procedures on 23 mobilized patients with multiple myeloma and lymphoma were analyzed...
July 21, 2016: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/27387375/histochemical-study-of-the-effects-on-abomasal-mucins-of-haemonchus-contortus-or-teladorsagia-circumcincta-infection-in-lambs
#10
H V Simpson, S Umair, V C Hoang, M S Savoian
Previously, chemical analysis of gastric fundic mucin showed that infection of sheep with Haemonchus contortus or Teladorsagia circumcincta changed the proportions of monosaccharides and decreased terminal mucin fucosylation and sialylation. To identify the effects of these parasites on the two mucin-secreting cell lineages, fundic and antral tissues were collected for histochemistry from 69 lambs aged from 3-4 to 9-10 months-of-age which had received a single infection of either H. contortus or T. circumcincta and euthanased at Day 21 or 28 post- infection respectively...
August 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27301272/lactobacillus-rhamnosus-gg-modulates-innate-signaling-pathway-and-cytokine-responses-to-rotavirus-vaccine-in-intestinal-mononuclear-cells-of-gnotobiotic-pigs-transplanted-with-human-gut-microbiota
#11
Haifeng Wang, Kan Gao, Ke Wen, Irving Coy Allen, Guohua Li, Wenming Zhang, Jacob Kocher, Xingdong Yang, Ernawati Giri-Rachman, Guan-Hong Li, Sherrie Clark-Deener, Lijuan Yuan
BACKGROUND: A better understanding of mechanisms underlying dose-effects of probiotics in their applications as treatments of intestinal infectious or inflammatory diseases and as vaccine adjuvant is needed. In this study, we evaluated the modulatory effects of Lactobacillus rhamnosus GG (LGG) on transplanted human gut microbiota (HGM) and on small intestinal immune cell signaling pathways in gnotobiotic pigs vaccinated with an oral attenuated human rotavirus (AttHRV) vaccine. RESULTS: Neonatal HGM transplanted pigs were given two doses of AttHRV on 5 and 15 days of age and were divided into three groups: none-LGG (AttHRV), 9-doses LGG (AttHRV + LGG9X), and 14-doses LGG (AttHRV + LGG14X) (n = 3-4)...
2016: BMC Microbiology
https://www.readbyqxmd.com/read/27292585/effect-of-testosterone-on-hepcidin-ferroportin-ferritin-and-iron-binding-capacity-in-patients-with-hypogonadotropic-hypogonadism-and-type-2-diabetes
#12
Sandeep Dhindsa, Husam Ghanim, Manav Batra, Nitesh D Kuhadiya, Sanaa Abuaysheh, Kelly Green, Antoine Makdissi, Ajay Chaudhuri, Paresh Dandona
CONTEXT: As the syndrome of hypogonadotropic hypogonadism (HH) is associated with anaemia and the administration of testosterone restores haematocrit to normal, we investigated the potential underlying mechanisms. DESIGN: Randomized, double-blind, placebo-controlled trial. METHODS: We measured basal serum concentrations of erythropoietin, iron, iron binding capacity, transferrin (saturated and unsaturated), ferritin and hepcidin and the expression of ferroportin and transferrin receptor (TR) in peripheral blood mononuclear cells (MNC) of 94 men with type 2 diabetes...
November 2016: Clinical Endocrinology
https://www.readbyqxmd.com/read/27250563/comparison-of-two-automated-mononuclear-cell-collection-systems-in-patients-undergoing-extracorporeal-photopheresis-a-prospective-crossover-equivalence-study
#13
Claudia Del Fante, Luigia Scudeller, Alberto Martinasso, Gianluca Viarengo, Cesare Perotti
BACKGROUND: Extracorporeal photopheresis (ECP) is an effective cell therapy employed in several diseases, including graft versus host disease (GVHD) and organ rejection. When ECP is performed using an off-line technique, mononuclear cell (MNC) collection by leukapheresis is necessary for further manipulation (addition of 8-methoxypsoralen and ultraviolet A irradiation before reinfusion to the patient). We report the results of the first crossover equivalence study on yield and purity of MNCs collected from patients undergoing ECP with two different automated systems: MNC and CMNC (working with intermittent and continuous-flow collection, respectively), released by Terumo BCT...
August 2016: Transfusion
https://www.readbyqxmd.com/read/27236676/isolation-culture-and-characterization-of-human-umbilical-cord-blood-derived-mesenchymal-stromal-cells
#14
Karen Bieback, Philipp Netsch
Umbilical cord blood (CB) is considered one of the youngest available sources of adult stem cells. Besides hematopoietic stem cells, CB has been shown to contain endothelial progenitor cells as well as mesenchymal stromal/stem cells (MSC). To isolate MSC from cord blood, CB is collected into a sterile bag containing the anticoagulant citrate-phosphate-dextrose (CPD). The CB is then processed by density-gradient centrifugation to obtain mononuclear cells (MNC). These are cultured until the outgrowth of fibroblastoid cell colonies appears...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27144073/knowledge-of-maternal-and-newborn-care-among-primary-level-health-workers-in-kapilvastu-district-of-nepal
#15
D Acharya, R Paudel, K Gautam, S Gautam, T Upadhyaya
BACKGROUND: Higher maternal and neonatal deaths are common in low- and middle-income countries; due to less access to skilled help. Adequate knowledge and skills on maternal and newborn care (MNC) of community health workers can improve maternal and newborn health. AIMS: To identify the knowledge of primary level health workers on some components of MNC. SUBJECTS AND METHODS: Respondents were selected using simple random sampling method. For collecting the data, enumerators visited health institutions for 2 months from 1(st) October to 31(st) November 2012, and structured interview schedule was used to gather the information...
January 2016: Annals of Medical and Health Sciences Research
https://www.readbyqxmd.com/read/27095367/comparison-between-intermittent-and-continuous-spectra-optia-leukapheresis-systems-for-autologous-peripheral-blood-stem-cell-collection
#16
Katharina Lisenko, Petra Pavel, Thomas Bruckner, Joe Puthenparambil, Michael Hundemer, Anita Schmitt, Mathias Witzens-Harig, Anthony D Ho, Patrick Wuchter
Terumo BCT recently introduced a new system for mononuclear cell (MNC) collection that uses a Spectra Optia apheresis machine equipped with a redesigned disposable kit and software program (version 11.2). It allows for the continuous collection of MNCs, unlike the original Spectra Optia system (version 7.2), which included a chamber for two-step cell separation. The aim of this study was to compare the two apheresis systems in regard to specific performance parameters. A retrospective data analysis of 150 patients who had undergone peripheral blood stem cell collection between March of 2014 and May of 2015 at our institution was performed...
April 20, 2016: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/27059201/cord-blood-collection-and-processing-with-hydroxyethyl-starch-or-non-hydroxyethyl-starch
#17
Svenja Schwandt, Lutz Korschgen, Svenja Peters, Gesine Kogler
BACKGROUND: Collection and processing characteristics influencing quality of cord blood (CB) units play an essential role to cord blood banks (CBBs). At many CBBs, volume reduction is performed using hydroxyethyl starch (HES) and the Sepax (Biosafe) automated cell processing system. Due to the withdrawal of HES from the European market, a validation of the nonHES protocol was performed. METHODS: This partially retrospective study identified CB characteristics such as gestational age and CB volume/cell count correlated with higher quality...
May 2016: Cytotherapy
https://www.readbyqxmd.com/read/26974703/in-vivo-and-in-vitro-evaluation-of-hydroxyapatite-nanoparticle-morphology-on-the-acute-inflammatory-response
#18
Shiuli Pujari-Palmer, Song Chen, Stefano Rubino, Hong Weng, Wei Xia, Håkan Engqvist, Liping Tang, Marjam Karlsson Ott
Biomedical implants have been widely used in bone repair applications. However, nanosized degradation products from these implants could elicit an inflammatory reaction, which may lead to implant failure. It is well known that the size, chemistry, and charge of these nanoparticles can modulate this response, but little is known regarding the role that the particle's morphology plays in inducing inflammation. The present study aims to investigate the effect of hydroxyapatite nanoparticle (HANPs) morphology on inflammation, in-vitro and in-vivo...
June 2016: Biomaterials
https://www.readbyqxmd.com/read/26880960/stem-cell-mobilization-with-g-csf-versus-cyclophosphamide-plus-g-csf-in-mexican-children
#19
José Eugenio Vázquez Meraz, José Arellano-Galindo, Armando Martínez Avalos, Emma Mendoza-García, Elva Jiménez-Hernández
Fifty-six aphaereses were performed in 23 pediatric patients with malignant hematological and solid tumors, following three different protocols for PBPC mobilization and distributed as follows: A: seventeen mobilized with 4 g/m(2) of cyclophosphamide (CFA) and 10 μg/kg/day of granulocyte colony stimulating factor (G-CSF), B: nineteen with CFA + G-CSF, and C: twenty only with G-CSF when the WBC count exceeded 10 × 10(9)/L. The average number of MNC/kg body weight (BW)/aphaeresis was 0.4 × 10(8) (0.1-1...
2016: Stem Cells International
https://www.readbyqxmd.com/read/26691087/influence-of-the-flood-pulse-on-the-reproduction-of-tocantinsia-piresi-miranda-ribeiro-and-auchenipterus-nuchalis-spix-agassiz-auchenipteridae-of-the-middle-xingu-river-brazil
#20
T M S Freitas, B S Prudente, V A Oliveira, M N C Oliveira, E G Prata, H Leão, L F A Montag
This study investigates the influence of the flood pulse on the reproductive biology of the auchenipterids Tocantisia piresi (Miranda Ribeiro, 1920) and Auchenipterus nuchalis (Spix & Agassiz, 1829) from the middle Xingu River in the Brazilian state of Pará. The specimens were collected every three months between April, 2012, and January, 2014, covering four distinct periods (flood, ebb, dry, and filling). The sex ratio, size at first maturity, gonadosomatic index, and condition factor were analysed in the two species, and evaluated in the context of the different hydrological periods...
August 2015: Brazilian Journal of Biology, Revista Brasleira de Biologia
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