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https://www.readbyqxmd.com/read/28925937/repetitive-immunosensor-with-a-fiber-optic-device-and-antibody-coated-magnetic-beads-for-semi-continuous-monitoring-of-escherichia-coli-o157-h7
#1
Midori Taniguchi, Hirokazu Saito, Kohji Mitsubayashi
A rapid and reproducible fiber-optic immunosensor for Escherichia coli O157:H7 (E. coli O157:H7) was described. The biosensor consisted of a flow cell, an optical fiber with a thin Ni layer, and a PC linked fluorometer. First, the samples with E. coli O157:H7 were incubated with magnetic beads coated with anti-E. coli O157:H7 antibodies and anti-E. coli O157:H7 antibodies labeled cyanine 5 (Cy5) to make sandwich complexes. Then the Cy5-(E. coli O157:H7)-beads were injected into a flow cell and pulled to the magnetized Ni layer on the optical fiber set in the flow cell...
September 19, 2017: Sensors
https://www.readbyqxmd.com/read/28925857/comparison-of-stored-red-blood-cell-washing-techniques-for-priming-extracorporeal-circuits
#2
Jun Sasaki, Christopher Tirotta, Hyunsoo Lim, Kathleen Kubes, Jane Salvaggio, Robert Hannan, Redmond Burke, Jorge Ojito
BACKGROUND: The aim of this study was to compare three different blood washing techniques and describe the differences for the composition of the washed red blood cells (RBC). METHODS: Stored RBCs less than 5 days old were washed using three different techniques. 1) Washing with normal saline with the COBE Model 2991 blood processor in the blood bank (BB-S). 2) Washing with normal saline with the Continuous AutoTransfusion System (C.A.T.S) in the operating room (OR-S)...
September 1, 2017: Perfusion
https://www.readbyqxmd.com/read/28917809/characterization-of-sodium-tripolyphosphate-and-sodium-citrate-dehydrate-residues-on-surfaces
#3
M Sadi Gurses, Can Erkey, Seda Kizilel, Alper Uzun
Sodium tripolyphosphate (STPP) and sodium citrate dihydrate (sodium citrate) are the most widely used components in detergent formulations. Here, we characterized these two components on glass surfaces to assess their possible exposures from white spots on dishwasher-washed dishes. Ultraviolet/visible near infrared spectroscopy (UV/Vis-NIR), Fourier transform infrared spectroscopy (FTIR) in the attenuated total reflectance mode (ATR-FTIR), Raman spectroscopy and laser ablation-inductively coupled plasma mass spectroscopy (LA-ICP-MS) were utilized to design calibration models for a range of STPP and sodium citrate concentrations 1-8% w/w) precipitated on glass surfaces...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28914471/wash-overexpression-enhances-cancer-stem-cell-properties-and-correlates-with-poor-prognosis-of-esophageal-carcinoma
#4
Lan Huang, Jingyao Lian, Xinfeng Chen, Guohui Qin, Yujia Zheng, Yi Zhang
There is increasing evidence that cytoskeleton remodeling is involved in cancer progression. Wiskott-Aldrich syndrome Protein (WASP) family represents a key regulator of actin cytoskeleton remodeling. However, the underlying mechanism of WASP family in cancer progression remains elusive. Here, we studied the role of WASP and SCAR Homolog (WASH), a recently identified WASP family member, in human esophageal squamous cell carcinoma (ESCC). Using three human ESCC cell lines, we found that WASH expression was significantly elevated in cancer stem-like cells enriched by sphere formation assay...
September 15, 2017: Cancer Science
https://www.readbyqxmd.com/read/28913873/improvement-of-decellularization-efficiency-of-porcine-aorta-using-dimethyl-sulfoxide-as-a-penetration-enhancer
#5
Selcan Guler, Halil M Aydin, Lan-Xin Lü, Ying Yang
Decellularization of tissues and organs enables researchers to obtain extracellular matrix (ECM) with the natural conformation and chemical composition of specific tissues. However, drawbacks exist such as the structural alteration of ECM or loss of some important components in ECM due to overexposure to chemicals during the decellularization process. In this study, porcine aorta was decellularized by sodium dodecyl sulfate (SDS). Dimethyl sulfoxide (DMSO) was used as a penetration enhancer in the decellularization process to enhance the penetration of SDS, consequently reducing the exposure time of SDS to treated tissues...
September 15, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28912578/tumour-acidosis-from-the-passenger-to-the-driver-s-seat
#6
REVIEW
Cyril Corbet, Olivier Feron
The high metabolic demand of cancer cells leads to an accumulation of H(+) ions in the tumour microenvironment. The disorganized tumour vasculature prevents an efficient wash-out of H(+) ions released into the extracellular medium but also favours the development of tumour hypoxic regions associated with a shift towards glycolytic metabolism. Under hypoxia, the final balance of glycolysis, including breakdown of generated ATP, is the production of lactate and a stoichiometric amount of H(+) ions. Another major source of H(+) ions results from hydration of CO2 produced in the more oxidative tumour areas...
September 15, 2017: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/28912099/antimicrobial-peptides-are-degraded-by-the-cytosolic-proteases-of-human-erythrocytes
#7
Charles G Starr, William C Wimley
Well-studied and promising antimicrobial peptides (AMPs), with potent bactericidal activity, in vitro, have yet to have a significant impact in human medicine beyond topical applications. We previously showed that interactions of AMPs with concentrated human erythrocytes inhibit many of them, and suggested that screens and assays should be done in their presence to mimic host cell inhibition. Here, we use AMPs to characterize the activity of proteases that are associated with human erythrocytes. The representative AMPs, ARVA and indolicidin, are degraded significantly during incubation with dilute, washed erythrocytes and yield a variety of degradation products, suggesting significant exopeptidase activity...
September 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28905449/lull-pgm-system-a-suitable-technique-to-improve-the-regenerative-potential-of-autologous-fat-grafting
#8
Paolo G Morselli, Federico A Giorgini, Claudia Pazzini, Claudio Muscari
Autologous fat grafting and methods of purification of harvested tissue have become one of the most current themes in regenerative medicine. The aim of this study was to evaluate the in vitro regenerative potential of abdomen lipoaspirates subjected to a combined washing-decantation purifying procedure, the Lull pgm System (Lull). Blood cells and stromal-vascular fraction (SVF) cells contained in the aspirates were investigated and compared with those obtained through more conventional fat-processing methods, i...
September 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28901892/the-effect-of-anthocyanin-supplementation-in-modulating-platelet-function-in-sedentary-population-a-randomised-double-blind-placebo-controlled-cross-over-trial
#9
Kiara Thompson, Holly Hosking, Wayne Pederick, Indu Singh, Abishek B Santhakumar
The anti-thrombotic properties of anthocyanin (ACN) supplementation was evaluated in this randomised, double-blind, placebo (PBO) controlled, cross-over design, dietary intervention trial in sedentary population. In all, sixteen participants (three males and thirteen females) consumed ACN (320 mg/d) or PBO capsules for 28 d followed by a 2-week wash-out period. Biomarkers of thrombogenesis and platelet activation induced by ADP; platelet aggregation induced by ADP, collagen and arachidonic acid; biochemical, lipid, inflammatory and coagulation profile were evaluated before and after supplementation...
September 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28900717/monitoring-of-oxidative-status-in-three-native-australian-species-during-cold-acclimation-and-cryopreservation
#10
Bryn Funnekotter, Louise Colville, Anja Kaczmarczyk, Shane R Turner, Eric Bunn, Ricardo L Mancera
Three wild species exhibited a significant reduction in antioxidants throughout the cryopreservation protocol, whilst the half-cell reduction potential became more oxidised. Antioxidant content recuperated in recovering shoot tips. Cryopreservation is the most efficient and cost-effective long-term storage solution for the conservation of a wide range of plant species and material. Changes in the levels of antioxidants during the process of cryopreservation are known to reduce post-cryogenic survival due to oxidative stress...
September 12, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28895623/comprehensive-analytics-of-actovegin%C3%A2-and-its-effect-on-muscle-cells
#11
Franz-Xaver Reichl, Lesca Miriam Holdt, Daniel Teupser, Gregor Schütze, Alan J Metcalfe, Reinhard Hickel, Christof Högg, Wilhelm Bloch
The ingredients of Actovegin(®) were analyzed and its effects on the muscle cell proliferation were investigated. C2C12 myoblasts were cultured in medium. Actovegin(®) was added in five different concentrations (1, 5, 25, 125, and 250 µg) to the differentiation medium. The formations of proliferation factor Ki67 and myosin heavy chains were measured by immunofluorescence. The first primary antibody was anti-Ki67 and anti-Mf20. Cells were washed and treated with the second fluorochrome. Thirty-one Actovegin(®) ingredients were found to contain significantly higher concentrations and twenty-nine ingredients were found to contain significantly lower concentrations, compared to the mean ranges as described in the literature for the normal physiological concentrations in human adult serum/plasma...
September 11, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28892079/retriever-is-a-multiprotein-complex-for-retromer-independent-endosomal-cargo-recycling
#12
Kerrie E McNally, Rebecca Faulkner, Florian Steinberg, Matthew Gallon, Rajesh Ghai, David Pim, Paul Langton, Neil Pearson, Chris M Danson, Heike Nägele, Lindsey L Morris, Amika Singla, Brittany L Overlee, Kate J Heesom, Richard Sessions, Lawrence Banks, Brett M Collins, Imre Berger, Daniel D Billadeau, Ezra Burstein, Peter J Cullen
Following endocytosis into the endosomal network, integral membrane proteins undergo sorting for lysosomal degradation or are retrieved and recycled back to the cell surface. Here we describe the discovery of an ancient and conserved multiprotein complex that orchestrates cargo retrieval and recycling and, importantly, is biochemically and functionally distinct from the established retromer pathway. We have called this complex 'retriever'; it is a heterotrimer composed of DSCR3, C16orf62 and VPS29, and bears striking similarity to retromer...
September 11, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28890781/thermo-responsive-cell-culture-carrier-effects-on-macrophage-functionality-and-detachment-efficiency
#13
Knut Rennert, Mirko Nitschke, Maria Wallert, Natalie Keune, Martin Raasch, Stefan Lorkowski, Alexander S Mosig
Harvesting cultivated macrophages for tissue engineering purposes by enzymatic digestion of cell adhesion molecules can potentially result in unintended activation, altered function, or behavior of these cells. Thermo-responsive polymer is a promising tool that allows for gentle macrophage detachment without artificial activation prior to subculture within engineered tissue constructs. We therefore characterized different species of thermo-responsive polymers for their suitability as cell substrate and to mediate gentle macrophage detachment by temperature shift...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28887473/complete-sequence-based-pathway-analysis-by-differential-on-chip-dna-and-rna-extraction-from-a-single-cell
#14
D van Strijp, R C M Vulders, N A Larsen, J Schira, L Baerlocher, M A van Driel, M Pødenphant, T S Hansen, A Kristensen, K U Mir, T Olesen, W F J Verhaegh, R Marie, P J van der Zaag
We demonstrate on-chip, differential DNA and RNA extraction from a single cell using a microfluidic chip and a two-stage lysis protocol. This method enables direct use of the whole extract, without additional washing steps, reducing sample loss. Using this method, the tumor driving pathway in individual cells from a colorectal cancer cell line was determined by applying a Bayesian computational pathway model to sequences obtained from the RNA fraction of a single cell and, the mutations driving the pathway were determined by analyzing sequences obtained from the DNA fraction of the same single cell...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28886443/antiplasmodial-activities-of-dyes-against-plasmodium-falciparum-asexual-and-sexual-stages-contrasted-uptakes-of-triarylmethanes-brilliant-green-green-s-e142-and-patent-blue-v-e131-by-erythrocytes
#15
Louis-Jérôme Leba, Jean Popovici, Yannick Estevez, Stéphane Pelleau, Eric Legrand, Lise Musset, Christophe Duplais
The search for safe antimalarial compounds acting against asexual symptom-responsible stages and sexual transmission-responsible forms of Plasmodium species is one of the major challenges in malaria elimination programs. So far, among current drugs approved for human use, only primaquine has transmission-blocking activity. The discovery of small molecules targeting different Plasmodium falciparum life stages remains a priority in antimalarial drug research. In this context, several independent studies have recently reported antiplasmodial and transmission-blocking activities of commonly used stains, dyes and fluorescent probes against P...
August 2, 2017: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/28884655/screening-for-the-presence-of-lipophilic-marine-biotoxins-in-shellfish-samples-using-the-neuro-2a-bioassay
#16
Marcia Bodero, Toine F H Bovee, Si Wang, Ron L A P Hoogenboom, Mirjam D Klijnstra, Liza Portier, Peter J M Hendriksen, Arjen Gerssen
The neuro-2a bioassay is considered as one of the most promising cell-based in vitro bioassays for the broad screening of seafood products for the presence of marine biotoxins. The neuro-2a assay has been shown to detect a wide array of toxins like paralytic shellfish poisons (PSPs), ciguatoxins, and also lipophilic marine biotoxins (LMBs). However, the neuro-2a assay is rarely used for routine testing of samples due to matrix effects that, for example, lead to false positives when testing for LMBs. As a result there are only limited data on validation and evaluation of its performance on real samples...
September 8, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28883764/prognostic-significance-diagnosis-and-treatment-in-patients-with-gastric-cancer-and-positive-peritoneal-washings-a-review-of-the-literature
#17
REVIEW
Radosław Lisiecki, Małgorzata Kruszwicka, Arkadiusz Spychała, Dawid Murawa
Peritoneal dissemination is a common consequence of a relapse following a radical surgical treatment of gastric cancer. The development of the disease in the peritoneum depends not only on its stage, but also on free cancer cells exfoliated from the tumor mass or from involved lymph nodes, and which are capable of being implanted in the peritoneum. According to the latest TNM (7 edition; 2010) classification, patients with free cancer cells in the peritoneal washings qualify for stage IV of the disease. Patients in whom free cancer cells were found during the operation - have a recurrence of gastric cancer - mainly in the peritoneum, and the majority of them die within two years of the diagnosis...
November 2017: Reports of Practical Oncology and Radiotherapy
https://www.readbyqxmd.com/read/28877350/oxidative-stress-increases-erythrocyte-sensitivity-to-shear-mediated-damage
#18
Antony P McNamee, Jarod T Horobin, Geoff D Tansley, Michael J Simmonds
Patients receiving mechanical circulatory support often present with heightened inflammation and free radical production associated with pre-existing conditions in addition to that which is due to blood interactions with nonbiological surfaces. The aim of this experimental laboratory study was to assess the deformability of red blood cells (RBC) previously exposed to oxygen free radicals and determine the susceptibility of these cells to mechanical forces. In the present study, RBC from 15 healthy donors were washed and incubated for 60 min at 37°C with 50 µM phenazine methosulfate (PMS; an agent that generates superoxide within RBC)...
September 6, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28870699/enhancing-recovery-of-recombinant-hepatitis-b-surface-antigen-in-lab-scale-and-large-scale-anion-exchange-chromatography-by-optimizing-the-conductivity-of-buffers
#19
Gol Mohammad Mojarrad Moghanloo, Maryam Khatami, Amin Javidanbardan, Seyed Nezamedin Hosseini
In biopharmaceutical science, ion-exchange chromatography (IEC) is a well-known purification technique to separate the impurities such as host cell proteins from recombinant proteins. However, IEC is one of the limiting steps in the purification process of recombinant hepatitis B surface antigen (rHBsAg), due to its low recovery rate (<50%). In the current study, we hypothesized that ionic strengths of IEC buffers are easy-to-control parameters which can play a major role in optimizing the process and increasing the recovery...
September 1, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28869110/anandamide-exerts-a-suppressive-effect-on-sperm-binding-to-oviduct-explants-through-cb1-receptors-in-the-water-buffalo-bubalus-bubalis
#20
Vimlesh Kumar, Arumugam Kumaresan, Puneeth Kumar D S, Sreela Lathika, Samiksha Nayak, Kaustubh Kishor Saraf, Pradeep Nag B S, Shivani Chhillar, Tirtha Kumar Datta, Tushar Kumar Mohanty
An endocannabinoid system comprising of Anandamide (AEA) and its receptor has been shown to play a role in sperm acquisition of fertilizing potential and sperm-oviduct interaction. In the present study, we assessed the effect of sperm pre-treatment with AEA or co-incubation of sperm-oviduct explants with AEA in the presence or absence of CB1 receptor antagonist (SR141716A) on sperm-oviduct binding in the water buffalo. Cryopreserved spermatozoa from 3 Murrah buffalo bulls (3 ejaculates from each bull) were utilized for the study...
October 2017: Animal Reproduction Science
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