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https://www.readbyqxmd.com/read/28107457/novel-analysis-of-immune-cells-from-nasal-microbiopsy-demonstrates-reliable-reproducible-data-for-immune-populations-and-superior-cytokine-detection-compared-to-nasal-wash
#1
Simon P Jochems, Katherine Piddock, Jamie Rylance, Hugh Adler, Beatriz F Carniel, Andrea Collins, Jenna F Gritzfeld, Carole Hancock, Helen Hill, Jesus Reiné, Alexandra Seddon, Carla Solórzano, Syba Sunny, Ashleigh Trimble, Angela D Wright, Seher Zaidi, Stephen B Gordon, Daniela M Ferreira
The morbidity and mortality related to respiratory tract diseases is enormous, with hundreds of millions of individuals afflicted and four million people dying each year. Understanding the immunological processes in the mucosa that govern outcome following pathogenic encounter could lead to novel therapies. There is a need to study responses at mucosal surfaces in humans for two reasons: (i) Immunological findings in mice, or other animals, often fail to translate to humans. (ii) Compartmentalization of the immune system dictates a need to study sites where pathogens reside...
2017: PloS One
https://www.readbyqxmd.com/read/28103277/protection-elicited-by-nasal-immunization-with-recombinant-pneumococcal-surface-protein-a-rpspa-adjuvanted-with-whole-cell-pertussis-vaccine-wp-against-co-colonization-of-mice-with-streptococcus-pneumoniae
#2
Rafaella O Tostes, Tasson C Rodrigues, Josefa B da Silva, Alessandra S Schanoski, Maria Leonor S Oliveira, Eliane N Miyaji
A promising alternative vaccine candidate to reduce the burden of pneumococcal diseases is the protein antigen PspA (Pneumococcal surface protein A). Since concomitant colonization with two or more pneumococcal strains is very common in children, we aimed to determine if immunization with PspA would be able to control co-colonization. We evaluated nasal immunization with recombinant PspA (rPspA) in a model of co-colonization with two strains expressing different PspAs. Mice were immunized intranasally with rPspAs from clades 1 to 4 (rPspA1, rPspA2, rPspA3 or rPspA4) using whole-cell pertussis vaccine (wP) as adjuvant...
2017: PloS One
https://www.readbyqxmd.com/read/28100859/-cotton-dam-wash-cytology-a-technique-for-diagnosis-and-prevention-of-dissemination-during-resection-of-metastatic-brain-tumors
#3
Koichi Mitsuya, Yoko Nakasu, Sachiyo Ono, Nakamasa Hayashi, Reiko Watanabe, Ichiro Ito
The management of brain metastases has been important in neurosurgical oncology. Resection of a brain metastasis carries an increased risk of leptomeningeal dissemination than other treatment modalities such as irradiation or pharmacotherapy. We have utilized intraoperative wash cytology of cotton patties covering the brain surface. The cytology information contributes to making a decision of postoperative whole brain radiation. We named the method as "cotton dam", that serves as a check and catch of neoplastic cells on the brain surface during resection surgery...
January 2017: No Shinkei Geka. Neurological Surgery
https://www.readbyqxmd.com/read/28099523/the-role-of-liquid-based-cytology-and-ancillary-techniques-in-the-peritoneal-washing-analysis-our-institutional-experience
#4
Esther Rossi, Tommaso Bizzarro, Maurizio Martini, Adhemar Longatto-Filho, Fernando Schmitt, Anna Fagotti, Giovanni Scambia, Gian Franco Zannoni
BACKGROUND: The cytological analysis of peritoneal effusions serves as a diagnostic and prognostic aid for either primary or metastatic diseases. Among the different cytological preparations, liquid based cytology (LBC) represents a feasible and reliable method ensuring also the application of ancillary techniques (i.e immunocytochemistry-ICC and molecular testing). METHODS: We recorded 10348 LBC peritoneal effusions between January 2000 and December 2014. They were classified as non-diagnostic (ND), negative for malignancy-NM, atypical-suspicious for malignancy-SM and positive for malignancy-PM...
2017: PloS One
https://www.readbyqxmd.com/read/28098879/cytokines-inducers-and-inhibitors-modulate-mmp-2-and-mmp%C3%A2-9-secretion-by-human-fanconi-anemia-immortalized-fibroblasts
#5
M W Roomi, T Kalinovsky, M Rath, A Niedzwiecki
Acute myeloid leukemia and head and neck squamous cell carcinomas are the major causes of mortality and morbidity in Fanconi anemia (FA) patients. Matrix metalloproteinases (MMPs), particularly MMP-2 and MMP-9, have been implicated in tumor invasion and metastasis. Various cytokines, mitogens, growth factors, inducers and inhibitors control MMP activities. We investigated the roles of these in the regulation of MMP-2 and MMP-9 in human immortalized fibroblasts from FA. Human FA immortalized fibroblast cell lines FA-A:PD220 and FA-D2:PD20 were grown in minimum essential medium (MEM) supplemented with 15% fetal bovine serum (FBS) and antibiotics in 24-well tissue culture plates...
January 16, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28098257/a-comprehensive-platform-for-the-analysis-of-ubiquitin-like-protein-modifications-using-in-vivo-biotinylation
#6
Lucia Pirone, Wendy Xolalpa, Jón Otti Sigurðsson, Juanma Ramirez, Coralia Pérez, Monika González, Ainara Ruiz de Sabando, Félix Elortza, Manuel S Rodriguez, Ugo Mayor, Jesper V Olsen, Rosa Barrio, James D Sutherland
Post-translational modification by ubiquitin and ubiquitin-like proteins (UbLs) is fundamental for maintaining protein homeostasis. Efficient isolation of UbL conjugates is hampered by multiple factors, including cost and specificity of reagents, removal of UbLs by proteases, distinguishing UbL conjugates from interactors, and low quantities of modified substrates. Here we describe bioUbLs, a comprehensive set of tools for studying modifications in Drosophila and mammals, based on multicistronic expression and in vivo biotinylation using the E...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28094033/vancomycin-added-to-the-wash-solution-of-the-cell-saver-effect-on-bacterial-contamination
#7
A Perez-Ferrer, E Gredilla-Díaz, J de Vicente-Sánchez, R Navarro-Suay, F Gilsanz-Rodríguez
OBJECTIVES: The aim of this study is to test whether the addition of a low-dose of antibiotic (vancomycin) to the wash solution (saline) of the cell-saver reduces the incidence of bacterial contamination of the autologous red blood cell (RBCs) concentrate recovered. MATERIAL AND METHOD: Experimental, randomized, double-blind, parallel group study performed on 20 consecutive patients scheduled for posterior spinal fusion surgery. Intraoperative bleeding was processed through a cell-saver: HaemoLite(®) 2+, in which the RBCs were washed according to randomization group, with saline (control group) or saline+10μg/ml(-1) vancomycin (vanco group)...
January 13, 2017: Revista Española de Anestesiología y Reanimación
https://www.readbyqxmd.com/read/28092842/blocking-tgf-%C3%AE-1-by-p17-peptides-attenuates-gastric-cancer-cell-induced-peritoneal-fibrosis-and-prevents-peritoneal-dissemination-in-vitro-and-in-vivo
#8
Zhi-Dong Lv, Wei-Jun Zhao, Li-Ying Jin, Wen-Juan Wang, Qian Dong, Na Li, Hui-Mian Xu, Hai-Bo Wang
Our previous study demonstrated that the peritoneal stroma environment favors proliferation of tumor cells by serving as a rich source of growth factors and chemokines known to be involved in tumor metastasis. In this study, we investigated the interaction between gastric cancer cells and peritoneal mesothelial cells, and determined the effects of TGF-β1 in this processing. Human peritoneal tissues and peritoneal wash fluid were obtained, which examined by hematoxylin and eosin staining or ELISA for measurements of TGF-β1 levels...
January 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28092639/lithographic-patterning-of-antibodies-by-direct-lift-off-and-improved-surface-adhesion
#9
Sankalp Verma, Vivek Verma
The inherent property of antibodies binding to their antigen with high specificity makes them a strong candidate for sensing and detection applications. Microscale patterning of antibodies is desired for the miniaturization of sensors and fundamental cell biology studies. However, existing methodologies to pattern antibodies at the microscale are multi-step. In this work, we demonstrate microscale patterning of antibodies on a glass coverslip in a single step photolithography process. The microscale features of the photoresist were generated on the coverslip using photolithography, and the antibody solution was incubated...
January 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28092380/chromatin-immunoprecipitation-in-microfluidic-droplets-towards-fast-and-cheap-analyses
#10
Bruno Teste, Jerome Champ, Arturo Londono-Vallejo, Stéphanie Descroix, Laurent Malaquin, Jean-Louis Viovy, Irena Draskovic, Guillaume Mottet
Genetic organization is governed by the interaction of DNA with histone proteins, and differential modifications of these proteins is a fundamental mechanism of gene regulation. Histone modifications are primarily studied through chromatin immunoprecipitation (ChIP) assays, however conventional ChIP procedures are time consuming, laborious and require a large number of cells. Here we report for the first time the development of ChIP in droplets based on a microfluidic platform combining nanoliter droplets, magnetic beads (MB) and magnetic tweezers (MT)...
January 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28091592/effective-extraction-of-arabidopsis-adherent-seed-mucilage-by-ultrasonic-treatment
#11
Xianhai Zhao, Lijun Qiao, Ai-Min Wu
The Arabidopsis seed coat is composed of two layers of mucilage, a water-soluble non-adherent outer layer and an adherent inner layer. The non-adherent mucilage can easily be extracted by gentle shaking. However, adherent mucilage is extremely difficult to dissociate from the seed coat. Despite various treatments to extract the adherent mucilage, including EDTA, ammonium oxalate, dilute alkali or acid washes, most of it remains on the seed coat. Here, we show for the first time the extraction of almost all of the adherent mucilage from the Arabidopsis seed coat...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28090282/traceless-solid-phase-%C3%AE-hydroxytropolone-synthesis
#12
Michael P D'Erasmo, Takashi Masaoka, Jennifer A Wilson, Errol M Hunte, John A Beutler, Stuart F J Le Grice, Ryan P Murelli
α-Hydroxytropolones are established inhibitors of several therapeutically relevant binuclear metalloenzymes, and thus lead drug targets for various human diseases. We have leveraged a recently-disclosed three-component oxidopyrylium cycloaddition in the first solid-phase synthesis of α-hydroxytropolones. We also showed that, while minor impurities exist after cleavage and aqueous wash, the semi-crude products display activity in HIV RT-associated RNaseH enzymatic and cell-based assays consistent with pure molecules made in solution phase...
September 1, 2016: MedChemComm
https://www.readbyqxmd.com/read/28086814/intestinal-helminth-co-infection-and-associated-factors-among-tuberculosis-patients-in-arba-minch-ethiopia
#13
Getaneh Alemu, Mohammedaman Mama
BACKGROUND: Helminths affect the outcome of tuberculosis by shifting cell mediated immune response to humoral and by total suppression of the host immune system. On the reverse, Mycobacterium infection favors immune escape of helminths. Therefore assessing helminth co-infection rate and predisposing factors in tuberculosis patients is mandatory to set strategies for better case management. METHODS: Facility based cross-sectional study was conducted in Arba Minch to assess the prevalence and associated factors of intestinal helminths among pulmonary tuberculosis patients from January to August, 2016...
January 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28081547/diagnosis-of-pancreaticobiliary-malignancy-by-detection-of-minichromosome-maintenance-protein-5-in-biliary-brush-cytology
#14
Margaret G Keane, Matthew T Huggett, Michael H Chapman, Gavin J Johnson, George J Webster, Douglas Thorburn, James Mackay, Stephen P Pereira
BACKGROUND: Biliary brush cytology is the standard method of evaluating biliary strictures, but is insensitive at detecting malignancy. In pancreaticobiliary cancer minichromosome maintenance replication proteins (MCM 2-7) are dysregulated in the biliary epithelium and MCM5 levels are elevated in bile samples. This study aimed to validate an immunocolorimetric ELISA assay for MCM5 as a pancreaticobiliary cancer biomarker in biliary brush samples. METHODS: Biliary brush specimens were collected prospectively at ERCP from patients with a biliary stricture...
January 12, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28081301/ethanol-induces-platelet-apoptosis
#15
Lei Liu, Mengxing Chen, Lili Zhao, Qing Zhao, Renping Hu, Jie Zhu, Rong Yan, Kesheng Dai
BACKGROUND: Alcohol abuse incurs severe medical conditions, such as thrombocytopenia and hemorrhage, but the pathogenesis is not totally understood. Alcohol has been reported to induce apoptosis in eukaryotic cells, such as hepatocyte, nerve cell, corneal fibroblasts. However, it is still unclear whether alcohol induces platelet apoptosis. METHODS: Washed human platelets were pretreated with ethanol (EtOH), and apoptotic events and platelet function were detected...
January 12, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/28076519/detection-value-of-free-cancer-cells-in-peritoneal-washing-in-gastric-cancer-a-systematic-review-and-meta-analysis
#16
Francisco Tustumi, Wanderley Marques Bernardo, Andre Roncon Dias, Marcus Fernando Kodama Pertille Ramos, Ivan Cecconello, Bruno Zilberstein, Ulysses Ribeiro-Júnior
Intraperitoneal free cancer cells in gastric adenocarcinoma are associated with a poor outcome. However, the true prognostic value of intraperitoneal free cancer cells is still unclear, leading to a lack of consensus in the management of gastric cancer. The aim of the present study is to perform a systematic review and meta-analysis to analyze intraperitoneal free cancer cells-positive patients with regard to tumor oncologic stage, recurrence, grade of cellular differentiation, and survival rates and to analyze the clinical significance of intraperitoneal free cancer cells with regard to prognosis...
December 1, 2016: Clinics
https://www.readbyqxmd.com/read/28068400/development-of-a-sensitive-and-specific-polyclonal-antibody-for-serological-detection-of-clavibacter-michiganensis-subsp-sepedonicus
#17
Włodzimierz Przewodowski, Agnieszka Przewodowska
The quarantine bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) causes bacterial ring rot (BRR) in potato but is difficult to detect, hampering the diagnosis of this disease. ELISA immunoassays have not been widely used to detect Cms because commercially available anti-Cms antibodies detect mainly EPS-producing bacteria and can fail to detect strains that do not produce EPS. In the current study, we developed a new type of polyclonal antibody that specifically detects Clavibacter michiganensis subsp...
2017: PloS One
https://www.readbyqxmd.com/read/28067898/clay-nanosheets-for-topical-delivery-of-rnai-for-sustained-protection-against-plant-viruses
#18
Neena Mitter, Elizabeth A Worrall, Karl E Robinson, Peng Li, Ritesh G Jain, Christelle Taochy, Stephen J Fletcher, Bernard J Carroll, G Q Max Lu, Zhi Ping Xu
Topical application of pathogen-specific double-stranded RNA (dsRNA) for virus resistance in plants represents an attractive alternative to transgenic RNA interference (RNAi). However, the instability of naked dsRNA sprayed on plants has been a major challenge towards its practical application. We demonstrate that dsRNA can be loaded on designer, non-toxic, degradable, layered double hydroxide (LDH) clay nanosheets. Once loaded on LDH, the dsRNA does not wash off, shows sustained release and can be detected on sprayed leaves even 30 days after application...
January 9, 2017: Nature Plants
https://www.readbyqxmd.com/read/28067649/treatment-of-laundry-wastewater-by-biological-and-electrocoagulation-methods
#19
Terelle Ramcharan, Ajay Bissessur
The present study describes an improvement in the current electrocoagulation treatment process and focuses on a comparative study for the clean-up of laundry wastewater (LWW) after each wash and rinse cycle by biological and electrocoagulation treatment methods. For biological treatment, the wastewater was treated with a Bacillus strain of aerobic bacteria especially suited for the degradation of fats, lipids, protein, detergents and hydrocarbons. Treatment of the LWW by electrocoagulation involved the oxidation of aluminium metal upon the application of a controlled voltage which produces various aluminium hydroxy species capable of adsorbing pollutants from the wastewater...
January 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28067514/click-and-fluoresce-a-bioorthogonally-activated-smart-probe-for-wash-free-fluorescent-labeling-of-biomolecules
#20
Xingyue Ji, Kaili Ji, Vayou Chittavong, Robert E Aghoghovbia, Mengyuan Zhu, Binghe Wang
Bioorthogonally activated smart probes greatly facilitate the selective labeling of biomolecules in living system. Herein, we described a novel type of smart probes with tunable reaction rates, high fluorescence turn-on ratio, and easy access. The practicality of such probes was demonstrated by selective labeling of lipid and hCAII in Hela cells.
January 9, 2017: Journal of Organic Chemistry
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