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https://www.readbyqxmd.com/read/29351208/preservation-method-and-phosphate-buffered-saline-washing-affect-the-acute-myeloid-leukemia-proteome
#1
Rebecca Wangen, Elise Aasebø, Andrea Trentani, Stein-Ove Døskeland, Øystein Bruserud, Frode Selheim, Maria Hernandez-Valladares
Acute myeloid leukemia (AML) primary cells can be isolated from peripheral blood, suspended with media containing bovine serum and cryoprotectant, and stored in liquid nitrogen before being processed for proteomic analysis by mass spectrometry (MS). The presence of bovine serum and human blood proteins in AML samples can hamper the identifications of proteins, and thereby reduce the proteome coverage of the study. Herein, we have established the effect of phosphate buffered saline (PBS) washing on AML patient samples stored in media...
January 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29341456/iron-pill-aspiration-cytologic-and-histologic-findings-of-a-potential-life-threatening-airway-injury-a-case-report-and-literature-review
#2
Vanessa Henriques, Diogo Costa Santos, Graça Lérias, Lucília Cáceres Monteiro
Iron pill-induced injury of bronchial mucosa is a complication following accidental aspiration of an iron tablet. Oral iron supplementation is a common therapy, particularly among advanced-age patients, who are more prone to aspiration. However, iron pill aspiration has been rarely reported in the literature, usually under the format of short case reports, with only 32 cases published in the literature. The cytologic features suspicious for this rare but potentially lethal entity have been seldom described...
January 16, 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29337420/a-proinflammatory-response-of-bovine-endometrial-epithelial-cells-to-active-sperm-in-vitro
#3
Ahmed Essam Elweza, Mohamed Aboul Ezz, Tomas J Acosta, Anup K Talukder, Takashi Shimizu, Hiroyuki Hayakawa, Masayuki Shimada, Kazuhiko Imakawa, Ahmed Hamed Zaghloul, Akio Miyamoto
In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechanism that activates polymorphonuclear neutrophils (PMNs). BUEC monolayers were co-cultured with different numbers of washed sperm obtained from cryopreserved semen (104 , 105 and 106 sperm/ml) for 3 h...
January 16, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29328343/in-situ-fluorescence-activation-of-dna-silver-nanoclusters-as-a-label-free-and-general-strategy-for-cell-nucleus-imaging
#4
Duo Li, Zhenzhen Qiao, Yanru Yu, Jinlu Tang, Xiaoxiao He, Hui Shi, Xiaosheng Ye, Yanli Lei, Kemin Wang
A facile, general and turn-on nucleus imaging strategy was first developed based on in situ fluorescence activation of C-rich dark silver nanoclusters by G-rich telomeres. After a simple incubation without washing, nanoclusters could selectively stain the nucleus with intense red luminescence, which was confirmed using fixed/living cells and several cell lines.
January 12, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29320456/preparative-purification-of-polyphenols-from-aronia-melanocarpa-chokeberry-with-cellular-antioxidant-and-antiproliferative-activity
#5
Ningxuan Gao, Yuehua Wang, Xinyao Jiao, Shurui Chou, Enhui Li, Bin Li
The aim of this study was the purification process of polyphenols from Aronia melanocarpa (chokeberry), and the purification parameters were optimised by adsorption and desorption tests. By comparing adsorption and desorption ability of polyphenols from chokeberry on six kinds of macroporous resin, XAD-7 resin was selected. Experiments prove that the best purification parameters of static adsorption and desorption were sample pH = 4.0 with 4 h of adsorption; and desorption solvent is 95% ethanol (pH = 7.0) with 2 h of desorption...
January 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29318370/the-metabolism-and-transport-of-1-5-anhydroglucitol-in-cells
#6
Lingwen Ying, Xiaojing Ma, Jun Yin, Yufei Wang, Xingxing He, Jiahui Peng, Yuqian Bao, Jian Zhou, Weiping Jia
AIMS: Our previous studies demonstrated that serum 1,5-anhydroglucitol (1,5-AG) levels increased slightly rather than declined after an acute glucose load. Therefore, the current study aims at exploring the transport and metabolic characteristics of 1,5-AG, as well as the effect of glucose on 1,5-AG transport. METHODS: Km and Vmax were determined to measure the affinity of glucose oxidase (GOD) and hexokinase (HK) for 1,5-AG and glucose. HepG2, C2C12, and primary mouse hepatocytes were incubated for 2 h with 1,5-AG at concentrations of 0, 80, and 160 μg/mL...
January 9, 2018: Acta Diabetologica
https://www.readbyqxmd.com/read/29316321/the-role-of-peritoneal-washings-in-the-diagnosis-of-endometriosis
#7
Richard L Cantley, Lorene Yoxtheimer, Stacy Molnar
Endometriosis, the presence of endometrial tissue outside the uterine corpus, is a common finding in reproductive age women. It is classically diagnosed based on the presence of at least two of the following elements: endometrial glands, endometrial stroma, and hemosiderin-laden macrophages (HLMs). Although a common finding in surgical pathology specimens at the time of gynecologic surgery, there is little literature on the role of pelvic washings in diagnosing endometriosis. Our study aimed to examine the characteristics of endometriosis in pelvic washings at the time of gynecologic surgery...
January 8, 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29313753/compared-to-an-oatmeal-breakfast-two-eggs-day-increased-plasma-carotenoids-and-choline-without-increasing-trimethyl-amine-n-oxide-concentrations
#8
Amanda Missimer, Maria Luz Fernandez, Diana M DiMarco, Gregory H Norris, Christopher N Blesso, Ana Gabriela Murillo, Marcela Vergara-Jimenez, Bruno S Lemos, Isabel Medina-Vera, Olga V Malysheva, Marie A Caudill
BACKGROUND: Habitual consumption of eggs has been hypothesized to positively modify biomarkers of cardiovascular disease risk through proposed antioxidant properties. OBJECTIVES: To examine this relationship, 50 young, healthy men and women were enrolled into a randomized crossover clinical intervention. METHODS: Participants consumed either 2 eggs per day or one packet of oatmeal a day for 4 weeks, followed by a 3-week wash-out and crossed over to the alternate breakfast...
January 9, 2018: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/29313337/improved-reversible-cross-linking-based-solid-phase-rna-extraction-for-pathogen-diagnostics
#9
Fei Zhao, Eun Yeong Lee, Yong Shin
In this study, we developed an amine-functionalized, diatomaceous earth-based, dimethyl suberimidate assisted (ADD) system as a novel binding strategy to improve solid-phase extraction method for rapid and simple purification of RNA from biological samples including human cells and pathogenic bacteria. This ADD system is based on reversible cross-linking reactions between RNA and the silica matrix. The formation of robust covalent bonds protects RNA from both the sufferance of washing steps and isolation with ribonuclease (RNase)-rich samples, leading to the extraction of higher quality RNA...
January 9, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29311110/long-lasting-in-vivo-effects-of-the-cannabinoid-cb1-antagonist-am6538
#10
Carol A Paronis, Girish R Chopda, Kiran Vemuri, Ani S Zakarian, Alexandros Makriyannis, Jack Bergman
AM6538 is a cannabinoid antagonist that binds CB1 receptors expressed in HEK-293 cells in a wash-resistant manner. The effects of AM6538 in live animals has not previously been established. We characterized the antagonist effects of AM6538 in male mice, using a warm-water tail-withdrawal assay, and in male squirrel monkeys trained to discriminate the CB1 agonist AM4054 from vehicle. The cannabinoid agonists WIN 55,212, Δ9-tetrahydrocannabinol (THC), and AM4054 all produced 100% maximum possible antinociceptive effects in mice following vehicle pretreatment...
January 8, 2018: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29297130/key-role-of-external-chloride-ions-in-regulation-of-fast-sodium-channels-in-rat-cerebellum-purkinje-neurons
#11
V L Zamoyski, V V Grigoriev
A decrease in the external chloride ion concentration to 4 mM caused a decrease in the fast sodium current to 85-100% in rat cerebellum Purkinje neurons in the whole-cell configuration using the patchclamp method. This effect did not depend on the main cation from the internal side of the cell membrane (120 mM of potassium or cesium) and also appeared when Cl- was substituted with sulfate (SO2-4) or phosphate (H2PO4-) anion outside of the cell. The effect was reversible after washing out the low chloride by the standard saline...
November 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29295701/evaluation-of-fibroblasts-cells-viability-and-adhesion-on-six-different-titanium-surfaces-an-in-vitro-experimental-study
#12
Sergio Alexandre Gehrke, Leticia Perez-Diaz, Berenice Anina Dedavid
BACKGROUND: The topographical surficial characteristics and properties of materials can induce different cell reactions regards to the viability and adhesion according to the recent patents. The aim of this in vitro experimental study was to evaluate the viability and adhesion of fibroblastic cells seeded on titanium disks with different surface topography. In addition, we revised all patents relating to sintering process titanium powder surface treatment for a of dental implants. METHODS: Fibroblast cells were cultured on 6 surfaces models: mA disks corresponding to titanium surfaces without additional treatment (machined surface) were compared with mB disks, where surfaces were sandblasting etching using aluminum oxide; mC disks where titanium oxide was used for sandblasting etching; mD disks where triple acid conditioning was used, mE disks with laser treated surface and mF disks which were made of titanium powder by a sintering process...
January 1, 2018: Recent Patents on Biotechnology
https://www.readbyqxmd.com/read/29290399/comprehensive-quantitative-analysis-of-fatty-acyl-coenzyme-a-species-in-biological-samples-by-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-harmonizing-hydrophilic-interaction-and-reversed-phase-chromatography
#13
László Abrankó, Gary Williamson, Samantha Gardner, Asimina Kerimi
Fatty acyl-Coenzyme A species (acyl-CoAs) are key biomarkers in studies focusing on cellular energy metabolism. Existing analytical approaches are unable to simultaneously detect the full range of short-, medium-, and long-chain acyl-CoAs, while chromatographic limitations encountered in the analysis of limited amounts of biological samples are an often overlooked problem. We report the systematic development of a UHPLC-ESI-MS/MS method which incorporates reversed phase (RP) and hydrophilic interaction liquid chromatography (HILIC) separations in series, in an automated mode...
December 23, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29287970/closed-system-manufacturing-of-cd19-and-dual-targeted-cd20-19-chimeric-antigen-receptor-t-cells-using-the-clinimacs-prodigy-device-at-an-academic-medical-center
#14
Fenlu Zhu, Nirav Shah, Huiqing Xu, Dina Schneider, Rimas Orentas, Boro Dropulic, Parameswaran Hari, Carolyn A Keever-Taylor
BACKGROUND AIMS: Multiple steps are required to produce chimeric antigen receptor (CAR)-T cells, involving subset enrichment or depletion, activation, gene transduction and expansion. Open processing steps that increase risk of contamination and production failure are required. This complex process requires skilled personnel and costly clean-room facilities and infrastructure. Simplified, reproducible CAR-T-cell manufacturing with reduced labor intensity within a closed-system is highly desirable for increased availability for patients...
December 26, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29286465/semi-automated-biopanning-of-bacterial-display-libraries-for-peptide-affinity-reagent-discovery-and-analysis-of-resulting-isolates
#15
Deborah A Sarkes, Justin P Jahnke, Dimitra N Stratis-Cullum
Biopanning bacterial display libraries is a proven technique for peptide affinity reagent discovery for recognition of both biotic and abiotic targets. Peptide affinity reagents can be used for similar applications to antibodies, including sensing and therapeutics, but are more robust and able to perform in more extreme environments. Specific enrichment of peptide capture agents to a protein target of interest is enhanced using semi-automated sorting methods which improve binding and wash steps and therefore decrease the occurrence of false positive binders...
December 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29285804/centrifugation-free-washing-a-novel-approach-for-removing-iga-from-stored-red-blood-cells
#16
Eszter Vörös, Nathaniel Z Piety, Briony C Strachan, Madeleine Lu, Sergey S Shevkoplyas
Washed red blood cells (RBCs) are indicated for immunoglobulin A (IgA) deficient recipients. Centrifugation-based cell processors commonly used by hospital blood banks cannot consistently reduce IgA below the recommended levels, hence double washing is frequently required. Here we describe a prototype of a simple, portable, disposable system capable of washing stored RBCs without centrifugation, while reducing IgA below 0.05 mg/dL in a single run. Samples from RBC units (n = 8, leukoreduced, 4-6 weeks storage duration) were diluted with normal saline to a hematocrit of 10%, and then washed using either the prototype washing system, or via conventional centrifugation...
December 29, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/29284662/junctional-trafficking-and-restoration-of-retrograde-signaling-by-the-cytoplasmic-ryr1-domain
#17
Alexander Polster, Stefano Perni, Dilyana Filipova, Ong Moua, Joshua D Ohrtman, Hicham Bichraoui, Kurt G Beam, Symeon Papadopoulos
The type 1 ryanodine receptor (RyR1) in skeletal muscle is a homotetrameric protein that releases Ca2+ from the sarcoplasmic reticulum (SR) in response to an "orthograde" signal from the dihydropyridine receptor (DHPR) in the plasma membrane (PM). Additionally, a "retrograde" signal from RyR1 increases the amplitude of the Ca2+ current produced by CaV1.1, the principle subunit of the DHPR. This bidirectional signaling is thought to depend on physical links, of unknown identity, between the DHPR and RyR1. Here, we investigate whether the isolated cytoplasmic domain of RyR1 can interact structurally or functionally with CaV1...
December 28, 2017: Journal of General Physiology
https://www.readbyqxmd.com/read/29279567/effects-of-cleaning-methods-for-custom-abutment-surfaces-on-gene-expression-of-human-gingival-fibroblasts
#18
Kazunori Nakajima, Tetsurou Odatsu, Ayano Shinohara, Koumei Baba, Yasuaki Shibata, Takashi Sawase
The aim of this study was to develop an effective method for cleaning implant abutments made by computer-aided design and computer-aided manufacturing techniques and to investigate the effect of decontamination in vitro. Briefly, a newly developed reagent (PK) and/or vacuum plasma (Plasma) were used to clean the surfaces of zirconia disks, and the effects of this decontamination were evaluated by X-ray photoelectron spectroscopy. Human gingival fibroblasts (HGFs) were cultured on sample disks for 6, 24, and 48 h...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29278600/ovarian-cancer-prevention-screening-and-early-detection-report-from-the-11th-biennial-ovarian-cancer-research-symposium
#19
Jeremy Chien, Elizabeth M Poole
OBJECTIVE: The aim of this study is to provide a summary report on recent research advances in ovarian cancer prevention, screening, and early detection that were presented at the 11th Biennial Ovarian Cancer Research Symposium in Seattle, Wash. METHODS: At the symposium, researchers from around the world participated in the poster, invited oral presentation and keynote presentation, and discussed the latest advances in the areas of cancer prevention, screening, and early detection...
November 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/29276506/escherichia-coli-o157-h7-converts-plant-derived-choline-to-glycine-betaine-for-osmoprotection-during-pre-and-post-harvest-colonization-of-injured-lettuce-leaves
#20
Russell A Scott, Roger Thilmony, Leslie A Harden, Yaguang Zhou, Maria T Brandl
Plant injury is inherent to the production and processing of fruit and vegetables. The opportunistic colonization of damaged plant tissue by human enteric pathogens may contribute to the occurrence of outbreaks of foodborne illness linked to produce. Escherichia coli O157:H7 (EcO157) responds to physicochemical stresses in cut lettuce and lettuce lysates by upregulation of several stress response pathways. We investigated the tolerance of EcO157 to osmotic stress imposed by the leakage of osmolytes from injured lettuce leaf tissue...
2017: Frontiers in Microbiology
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