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https://www.readbyqxmd.com/read/28727605/in-vitro-compatibility-of-contact-lenses-with-corneal-epithelial-cells
#1
Ajay K Vijay, Zohra Fadli, Carol Lakkis, Chantal Coles-Brennan, Mark D P Willcox
PURPOSE: To determine the interaction of contact lenses of different materials with corneal epithelial cells grown in tissue culture. METHODS: Two different corneal epithelial cell lines were grown to confluence in culture media. Two hydrogel contact lenses with and without polyvinylpyrrolidone (PVP) {1-DAY ACUVUE MOIST (1-Day ACUVUE [hydrogel lenses]) and a silicone hydrogel contact lens, AIR OPTIX NIGHT & DAY} were removed from their blister packs, washed in phosphate-buffered saline, and applied to the cells...
July 18, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28727490/comparison-of-the-release-of-micrornas-and-extracellular-vesicles-from-platelets-in-response-to-different-agonists
#2
Ashley R Ambrose, Mohammed A Alsahli, Sameer A Kurmani, Alison H Goodall
On activation platelets release microRNAs and extracellular vesicles (EV) into circulation. The release of EV from platelets has been shown to be dependent on the agonist; in this study, we investigated whether the microRNA profile or EV released from platelets was also agonist specific. Washed platelets from healthy subjects were maximally stimulated with agonists specific for the receptors for collagen (Glycoprotein VI (GPVI)), thrombin (PAR1/PAR4), or ADP (P2Y1/P2Y12) with/without inhibiting secondary mediators, using aspirin to block cyclooxygenase-1 and apyrase to remove ADP...
July 20, 2017: Platelets
https://www.readbyqxmd.com/read/28726681/a-novel-cylindrical-microwell-featuring-inverted-pyramidal-opening-for-efficient-cell-spheroid-formation-without-cell-loss
#3
Jae Min Cha, HyungDal Park, Eun Kyoung Shin, Ji Hee Sung, Ockchul Kim, Woohyun Jung, Oh Young Bang, Jinseok Kim
Spheroid cultures have been often used to simulate and understand in situ biological occurrences with potential to be further applied to therapeutic approaches, such as cell transplantation. However, traditional lab-scale techniques hardly reached the needed large scale production of cell spheroids, thus limiting their versatility in many biomedical fields. Microscale technologies have rapidly improved in the last decade, and contributed to the large scale production of cell spheroids with high controllability and reproducibility...
July 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28724312/in-vitro-skin-decontamination-of-paraoxon-wet-type-cleansing-effect-of-selected-detergents
#4
Ruzena Pavlikova, Jan Misik, Jiri Cabal, Jan Marek, Kamil Kuca
The aim of this study was to determine optimal conditions for in vitro skin decontamination using water and detergents as decontamination agents and to test the cleansing efficiency of selected detergents. Experiments were performed using a peristaltic pump for showering of pig skin in modified static diffision cells. Several conditions were tested including different flow rates (from 5 to 33 ml.s(-1)), quantity of rinsing fluid (from 40 to 400 ml), and concentration of detergents (2; 5; 10%). Further, several types of detergents/commercial decontamination agents were evaluated under the selected conditions to find the most effective means of decontamination...
July 20, 2017: Cutaneous and Ocular Toxicology
https://www.readbyqxmd.com/read/28723708/subclinical-herpesvirus-shedding-among-hiv-1-infected-men-on-antiretroviral-therapy
#5
Arcadio Agudelo-Hernandez, Yue Chen, Arlene Bullotta, William G Buchanan, Cynthia R Klamar-Blain, Luann Borowski, Sharon A Riddler, Charles R Rinaldo, Bernard Jc Macatangay
DESIGN: We evaluated the subclinical shedding of 6 different herpesviruses in antiretroviral drug-treated HIV(+) men who have sex with men (MSM), and determined how this is associated with markers of inflammation and immune activation. METHODS: We obtained blood, semen, throat washing, urine, and stool from 15 antiretroviral-treated HIV-1-infected MSM with CD4 T cell reconstitution, and 12 age-matched HIV(-) MSM from the Multicenter AIDS Cohort Study at 4 timepoints over 24 weeks to measure DNA levels of cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus 1 and 2, human herpesvirus 6 (HHV6), and HHV8...
July 18, 2017: AIDS
https://www.readbyqxmd.com/read/28720107/reversible-bacterial-immobilization-based-on-the-salt-dependent-adhesion-of-the-bacterionanofiber-protein-ataa
#6
Shogo Yoshimoto, Yuki Ohara, Hajime Nakatani, Katsutoshi Hori
BACKGROUND: Immobilization of microbial cells is an important strategy for the efficient use of whole-cell catalysts because it simplifies product separation, enables the cell concentration to be increased, stabilizes enzymatic activity, and permits repeated or continuous biocatalyst use. However, conventional immobilization methods have practical limitations, such as limited mass transfer in the inner part of a gel, gel fragility, cell leakage from the support matrix, and adverse effects on cell viability and catalytic activity...
July 18, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28715380/production-of-chemicals-by-klebsiella-pneumoniae-using-bamboo-hydrolysate-as-feedstock
#7
Dong Wei, Jinjie Gu, Zhongxi Zhang, Chenhong Wang, Dexin Wang, Chul Ho Kim, Biao Jiang, Jiping Shi, Jian Hao
Bamboo is an important biomass, and bamboo hydrolysate is used by Klebsiella pneumoniae as a feedstock for chemical production. Here, bamboo powder was pretreated with NaOH and washed to a neutral pH. Cellulase was added to the pretreated bamboo powder to generate the hydrolysate, which contained 30 g/L glucose and 15 g/L xylose and was used as the carbon source to prepare a medium for chemical production. When cultured in microaerobic conditions, 12.7 g/L 2,3-butanediol was produced by wildtype K. pneumoniae...
June 29, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28709438/surgical-treatment-for-mediastinal-abscess-induced-by-endobronchial-ultrasound-guided-transbronchial-needle-aspiration-a-case-report-and-literature-review
#8
Yujiro Yokoyama, Takahiro Nakagomi, Daichi Shikata, Rumi Higuchi, Toshio Oyama, Taichiro Goto
BACKGROUND: Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a useful and less invasive procedure for the definitive diagnosis of mediastinal and hilar lymph nodes. However, infectious complications can occur after EBUS-TBNA, although they are extremely rare. CASE PRESENTATION: A 66-year-old man with necrotic and swollen lower paratracheal lymph nodes underwent EBUS-TBNA. A mediastinal abscess developed 9 days post-procedure. Surgical drainage and debridement of the abscess were performed along with lymph node biopsy followed by daily washing of the thoracic cavity...
July 14, 2017: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28706747/adrenal-rest-tumor-of-the-liver-preoperatively-diagnosed-as-hepatocellular-carcinoma
#9
Megumu Enjoji, Katsuya Sanada, Ryota Seki, Takashi Ito, Masato Maeda
BACKGROUND: Hepatic adrenal rest tumors are rare and show similar findings to hepatocellular carcinoma (HCC). It is difficult to distinguish an adrenal rest tumor from HCC due to radiological similarity. We report a case of an adrenal rest tumor in the liver that mimicked HCC radiologically. CASE PRESENTATION: A 67-year-old female was referred to our hospital due to the finding of a hepatic mass. Enhanced computed tomography revealed a 17 mm well-defined tumor that was enhanced in the arterial phase and washed out in the portal and delayed phase in the posterosuperior subsegment of the right hepatic lobe, and HCC was suspected...
2017: Case Reports in Surgery
https://www.readbyqxmd.com/read/28703788/preparation-of-a-trp-bodipy-fluorogenic-amino-acid-to-label-peptides-for-enhanced-live-cell-fluorescence-imaging
#10
Lorena Mendive-Tapia, Ramon Subiros-Funosas, Can Zhao, Fernando Albericio, Nick D Read, Rodolfo Lavilla, Marc Vendrell
Fluorescent peptides are valuable tools for live-cell imaging because of the high specificity of peptide sequences for their biomolecular targets. When preparing fluorescent versions of peptides, labels must be introduced at appropriate positions in the sequences to provide suitable reporters while avoiding any impairment of the molecular recognition properties of the peptides. This protocol describes the preparation of the tryptophan (Trp)-based fluorogenic amino acid Fmoc-Trp(C2-BODIPY)-OH and its incorporation into peptides for live-cell fluorescence imaging-an approach that is applicable to most peptide sequences...
August 2017: Nature Protocols
https://www.readbyqxmd.com/read/28701824/liquid-based-and-conventional-cytology-for-bronchial-washings-bronchoalveolar-lavages-in-the-diagnosis-of-malignancy-an-institutional-experience
#11
Abha Thakur, Pooja Bakshi, Gagandeep Kaur, Kusum Verma
BACKGROUND: Liquid-based cytology (LBC) has been developed as an alternative for conventional cytology (CC) in cervical smears. It is now increasingly being used all over the world for cervical cancer screening. However, its role and diagnostic accuracy in bronchial wash (BW)/bronchoalveolar lavage (BAL) specimens remains undetermined. AIMS: To assess and compare the diagnostic performance and accuracy of LBC with CC for detecting malignancy in bronchial specimens...
July 2017: Journal of Cytology
https://www.readbyqxmd.com/read/28699666/apoptosis-inhibition-of-atlantic-salmon-salmo-salar-peritoneal-macrophages-by-piscirickettsia-salmonis
#12
S Díaz, M E Rojas, M Galleguillos, C Maturana, P I Smith, F Cifuentes, I Contreras, P A Smith
To improve the understanding of the piscirickettsiosis pathogenesis, the in vivo apoptosis modulation of peritoneal macrophages and lymphocytes was studied in juvenile Salmo salar intraperitoneally injected with Piscirickettsia salmonis. Five fish were sampled at post-exposure days 1, 5, 8 (preclinical), 20 (clinical) and 40 (post-clinical period of the disease), and the leucocytes of their coelomic washings were analysed by flow cytometry (using the JC-1 cationic dye), TUNEL and cytology to detect apoptotic cells...
July 12, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28698666/modelling-person-to-person-transmission-in-an-enterovirus-a71-orally-infected-hamster-model-of-hand-foot-and-mouth-disease-and-encephalomyelitis
#13
Win Kyaw Phyu, Kien Chai Ong, Kum Thong Wong
Enterovirus A71 (EV-A71) causes hand-foot-and-mouth disease (HFMD), which may be complicated by fatal encephalomyelitis. Although fecal-oral or oral-oral routes are important in person-to-person transmission, how viral shedding and exposure may predispose individuals to infection remains unknown. We investigated person-to-person transmission by using a model of HFMD and encephalomyelitis based on EV-A71 oral infection of 2-week-old hamsters. Animals (index animals) infected with 10(4) 50% cell culture infective doses of virus uniformly developed severe disease four days post-infection (dpi), whereas littermate contacts developed severe disease after six to seven days of exposure to index animals...
July 12, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28697458/uptake-and-toxicity-of-cuo-nanoparticles-to-daphnia-magna-varies-between-indirect-dietary-and-direct-waterborne-exposures
#14
Fan Wu, Amy Bortvedt, Bryan J Harper, Lauren E Crandon, Stacey L Harper
Research examining the direct and indirect ecological effects of nanomaterials in aquatic ecosystems is important for developing a more realistic understanding of the environmental implications of nanotechnology. Copper oxide nanoparticles (CuO NPs) are being used extensively in many industries but are considered highly toxic to aquatic species residing in surface waters. Few studies have addressed whether CuO NPs can be transferred through the aquatic food chain, and if such indirect exposure to nanomaterials impacts their toxicity...
June 26, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28697438/assessment-of-the-correlation-among-antibiotic-resistance-adherence-to-abiotic-and-biotic-surfaces-invasion-and-cytotoxicity-of-pseudomonas-aeruginosa-isolated-from-diseased-gilthead-sea-bream
#15
Faouzi Lamari, Ibtissem Chakroun, Sami Rtimi
Improper uses of antibiotics to treat fish disease pose an increase of multidrug resistance in Pseudomonas aeruginosa. In order to escape host antimicrobial agents and induce cytotoxicity, different virulence properties are needed by these bacteria such as, biofilm formation, adhesion and invasion ability. This study was conducted to isolate Pseudomonas aeruginosa from diseased cultured gilthead sea bream. Seventeen isolates of Pseudomonas aeruginosa were identified by PCR. All of the isolates tested were susceptible to Gentamicin and Ciprofloxacin...
July 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28694218/constitutive-expression-of-human-gastric-lipase-in-pichia-pastoris-and-site-directed-mutagenesis-of-key-lid-stabilizing-residues
#16
Laura Sams, Sawsan Amara, Almahdi Chakroun, Sébastien Coudre, Julie Paume, Jacqueline Giallo, Frédéric Carrière
The cDNA encoding human gastric lipase (HGL) was integrated into the genome of Pichia pastoris using the pGAPZα A transfer vector. The HGL signal peptide was replaced by the yeast α-factor to achieve an efficient secretion. Active rHGL was produced by the transformed yeast but its levels and stability were dependent on the pH. The highest activity was obtained upon buffering the culture medium at pH5, a condition that allowed preserving enzyme activity over time. A large fraction (72±2%) of secreted rHGL remained however bound to the yeast cells, and was released by washing the cell pellet with an acid glycine-HCl buffer (pH2...
July 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28689156/first-void-urine-a-potential-biomarker-source-for-triage-of-high-risk-human-papillomavirus-infected-women
#17
REVIEW
Severien Van Keer, Jade Pattyn, Wiebren A A Tjalma, Xaveer Van Ostade, Margareta Ieven, Pierre Van Damme, Alex Vorsters
Great interest has been directed towards the use of first-void urine as a liquid biopsy for high-risk human papillomavirus DNA testing. Despite the high correlations established between urinary and cervical infections, human papillomavirus testing is unable to distinguish between productive and transforming high-risk infections that have the tendency to progress to cervical cancer. Thus far, investigations have been primarily confined to the identification of biomarkers for triage of high-risk human papillomavirus-positive women in cervicovaginal specimens and tissue biopsies...
June 27, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28687674/anti-inflammatory-%C3%AF-3-endocannabinoid-epoxides
#18
Daniel R McDougle, Josephine E Watson, Amr A Abdeen, Reheman Adili, Megan P Caputo, John E Krapf, Rodney W Johnson, Kristopher A Kilian, Michael Holinstat, Aditi Das
Clinical studies suggest that diets rich in ω-3 polyunsaturated fatty acids (PUFAs) provide beneficial anti-inflammatory effects, in part through their conversion to bioactive metabolites. Here we report on the endogenous production of a previously unknown class of ω-3 PUFA-derived lipid metabolites that originate from the crosstalk between endocannabinoid and cytochrome P450 (CYP) epoxygenase metabolic pathways. The ω-3 endocannabinoid epoxides are derived from docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) to form epoxyeicosatetraenoic acid-ethanolamide (EEQ-EA) and epoxydocosapentaenoic acid-ethanolamide (EDP-EA), respectively...
July 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28686152/washing-in-hypotonic-saline-reduces-the-fraction-of-irreversibly-damaged-cells-in-stored-blood-a-proof-of-concept-study
#19
Hui Xia, Grishma Khanal, Briony C Strachan, Eszter Vörös, Nathaniel Z Piety, Sean C Gifford, Sergey S Shevkoplyas
BACKGROUND: During hypothermic storage, a substantial fraction of red blood cells (RBCs) transforms from flexible discocytes to rigid sphero-echinocytes and spherocytes. Infusion of these irreversibly-damaged cells into the recipient during transfusion serves no therapeutic purpose and may contribute to adverse outcomes in some patients. In this proof-of-concept study we describe the use of hypotonic washing for selective removal of the irreversibly-damaged cells from stored blood. MATERIALS AND METHODS: Stored RBCs were mixed with saline of various concentrations to identify optimal concentration for inducing osmotic swelling and selective bursting of spherical cells (sphero-echinocytes, spherocytes), while minimising indiscriminate lysis of other RBCs...
May 11, 2017: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/28686044/anti-biofilm-properties-of-a-mupirocin-spray-formulation-against-escherichia-coli-wound-infections
#20
Dhamodharan Bakkiyaraj, Rutthapol Sritharadol, Alwar Ramanujam Padmavathi, Titpawan Nakpheng, Teerapol Srichana
Mupirocin ointment is a widely used topical drug for the treatment of bacterial skin infections. However, ointments have some limitations which motivated the development of a film forming spray of mupirocin. Mupirocin spray (2%) was formulated with Eudragit E100 as a film forming agent and tested for its antibacterial and anti-biofilm activities against Escherichia coli, a skin pathogen causing wound and surgical site infections. Treatment with mupirocin spray resulted in significant antibacterial and anti-biofilm activities (inhibition and disruption) with single spray and sub-actual dose concentrations at par with the commercial ointment concentration...
July 7, 2017: Biofouling
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