Philipp Mauker, Daniela Beckmann, Annabel Kitowski, Constanze Heise, Chantal Wientjens, Andrew J Davidson, Simone Wanderoy, Gabin Fabre, Angelika B Harbauer, Will Wood, Christoph Wilhelm, Julia Thorn-Seshold, Thomas Misgeld, Martin Kerschensteiner, Oliver Thorn-Seshold
Selectively labeling cells with damaged membranes is needed not only for identifying dead cells in culture, but also for imaging membrane barrier dysfunction in pathologies in vivo . Most membrane permeability stains are permanently colored or fluorescent dyes that need washing to remove their non-uptaken extracellular background and reach good image contrast. Others are DNA-binding environment-dependent fluorophores, which lack design modularity, have potential toxicity, and can only detect permeabilization of cell volumes containing a nucleus (i...
April 9, 2024: Journal of the American Chemical Society