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https://www.readbyqxmd.com/read/28816366/modification-of-the-paris-system-for-urinary-tract-washing-specimens-using-diagnostic-cytological-features
#1
J Suh, H Go, C Sung, S Baek, H Hwang, S Jeong, Y Cho
OBJECTIVE: The Paris System (TPS), which was recently introduced, emphasised the key features of malignant urine cytology: a high nuclear to cytoplasmic ratio, nuclear hyperchromasia, irregular nuclear membranes and coarse chromatin. Although other diagnostic features have been described, the diagnostic significance of such features and their application to TPS have not been fully defined for urinary tract washing specimens. METHODS: A total of 142 cases of urinary tract washing specimens with corresponding surgical pathology samples were examined for the key features of TPS and 13 previously described features...
August 17, 2017: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/28816288/-recombinant-human-transforming-growth-factor-%C3%AE-1-promotes-dental-pulp-stem-cells-proliferation-and-mineralization
#2
W Q Jia, Y M Zhao, L H Ge
OBJECTIVE: To explore suitable concentration of recombinant human transforming growth factor β1 (rhTGF-β1) usage and study the effect of rhTGF-β1 on differentiation of dental pulp stem cells (DPSCs). METHODS: DPSCs were isolated from the undiseased third molars of people aged 18-25 years and cultured according to instructions in vitro. Different concentrations (1 , 6 , 10 μg/L) of rhTGF-β1 were added to the culture medium to examine DPSCs proliferation by CCK-8 (cell counting kit-8) assay...
August 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28815010/development-and-characterisation-of-a-low-concentration-sodium-dodecyl-sulphate-decellularised-porcine-dermis
#3
Jack A Helliwell, Daniel S Thomas, Vaia Papathanasiou, Shervanthi Homer-Vanniasinkam, Amisha Desai, Louise M Jennings, Paul Rooney, John N Kearney, Eileen Ingham
The aim of this study was to adapt a proprietary decellularisation process for human dermis for use with porcine skin. Porcine skin was subject to: sodium chloride (1 M) to detach the epidermis, trypsin paste to remove hair follicles, peracetic acid (0.1% v/v) disinfection, washed in hypotonic buffer and 0.1% (w/v) sodium dodecyl sulphate in the presence of proteinase inhibitors followed by nuclease treatment. Cellular porcine skin, decellularised porcine and human dermis were compared using histology, immunohistochemistry, GSL-1 lectin (alpha-gal epitope) staining, biochemical assays, uniaxial tensile and in vitro cytotoxicity tests...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28809821/gradient-strain-chip-for-stimulating-cellular-behaviors-in-cell-laden-hydrogel
#4
Hsin-Yi Hsieh, Chiao-Wen Chu, Ming-Hsuan Chiu, Shueh-Yao Chu, Tsu-Wei Huang, Fan-Gang Tseng
Artificial guidance for cellular alignment is a hot topic in the field of tissue engineering. Most of the previous research has investigated single strain-induced cellular alignment on a cell-laden hydrogel by using complex experimental processes and mass controlling systems, which are usually associated with contamination issues. Thus, in this article, we propose a simple approach to building a gradient static strain using a fluidic chip with a plastic PDMS cover and a UV transparent glass substrate for the stimulation of cellular behavior in a 3D hydrogel...
August 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28806071/contrasting-effects-of-dissolved-organic-matter-on-mercury-methylation-by-g-sulfurreducens-pca-and-d-desulfuricans-nd132
#5
Linduo Zhao, Hongmei Chen, Xia Lu, Hui Lin, Geoff A Christensen, Eric M Pierce, Baohua Gu
Natural dissolved organic matter (DOM) affects mercury (Hg) redox reactions and anaerobic microbial Hg methylation in the environment. Several studies have shown that DOM can enhance Hg methylation, especially under sulfidic conditions, whereas others show that DOM inhibits Hg methylation due to strong Hg-DOM complexation. In this study, we investigated and compared the effects of DOM on Hg methylation by an iron-reducing bacterium Geobacter sulfurreducens PCA and a sulfate-reducing bacterium Desulfovibrio desulfuricans ND132 under non-sulfidic conditions...
August 14, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28803543/bodipy-based-dye-for-no-wash-live-cell-staining-and-imaging
#6
Alexey A Pakhomov, Igor E Deyev, Natalia M Ratnikova, Stepan P Chumakov, Veronika B Mironiuk, Yuriy N Kononevich, Aziz M Muzafarov, Vladimir I Martynov
In nonpolar solvents, hydrophobic organic fluorophores often show bright fluorescence, whereas in polar media, they usually suffer from aggregation-caused quenching (ACQ). Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off.
August 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28800826/effect-of-thyme-essential-oil-and-lactococcus-lactis-cbm21-on-the-microbiota-composition-and-quality-of-minimally-processed-lamb-s-lettuce
#7
Lorenzo Siroli, Francesca Patrignani, Diana I Serrazanetti, Pamela Vernocchi, Federica Del Chierico, Alessandra Russo, Sandra Torriani, Lorenza Putignani, Fausto Gardini, Rosalba Lanciotti
The main aim of this work was to evaluate, at pilot scale in an industrial environment, the effects of the biocontrol agent Lactococcus lactis CBM21 and thyme essential oil compared to chlorine, used in the washing step of fresh-cut lamb's lettuce, on the microbiota and its changes in relation to the time of storage. The modification of the microbial population was studied through pyrosequencing in addition to the traditional plate counts. In addition, the volatile molecule and sensory profiles were evaluated during the storage...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28799674/ultrastructural-characteristics-of-ovine-bone-marrow-derived-mesenchymal-stromal-cells-cultured-with-a-silicon-stabilized-tricalcium-phosphate-bioceramic
#8
Salvatore Desantis, Gianluca Accogli, Janina Burk, Sara Zizza, Maria Mastrodonato, Edda G Francioso, Roberta Rossi, Antonio Crovace, Leonardo Resta
Bioceramics are being used in experimental bone engineering application in association with bone marrow derived mesenchymal stem cells (BM-MSCs) as a new therapeutic tool, but their effects on the ultrastructure of BM-MSCs are yet unknown. In this study we report the morphological features of ovine (o)BM-MSCs cultured with Skelite, a resorbable bioceramic based on silicon stabilized tricalcium phosphate (SiTCP), able to promote the repair of induced bone defect in sheep model. oBM-MSCs were isolated from the iliac crest, cultured until they reached near-confluence and incubated with SiTCP...
August 11, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28795150/the-effects-of-tacrolimus-on-t-cell-proliferation-are-short-lived-a-pilot-analysis-of-immune-function-testing
#9
Benjamin L Laskin, Jing Jiao, H Jorge Baluarte, Sandra Amaral, Susan L Furth, Tatiana Akimova, Wayne W Hancock, Matthew H Levine, Peter P Reese, Ulf H Beier
BACKGROUND: Optimal immunosuppression after organ transplant should balance the risks of rejection, infection, and malignancy while minimizing barriers to adherence including frequent or time-sensitive dosing. There is currently no reliable immune function assay to directly measure the degree of immunosuppression after transplantation. METHODS: We developed an immune function assay to mea//sure T-cell proliferation after exposure to immunosuppression in vivo. We tested the assay in mice, and then piloted the approach using single time point samples, 11 pediatric kidney transplant recipients prescribed tacrolimus, mycophenolate, and prednisone 6 months to 5 years posttransplant, with no history of rejection, opportunistic infection, or cancer...
August 2017: Transplantation Direct
https://www.readbyqxmd.com/read/28794158/intracellular-rebinding-of-transition-state-analogues-provides-extended-in-vivo-inhibition-lifetimes-on-human-purine-nucleoside-phosphorylase
#10
Sara T Gebre, Scott A Cameron, Lei Li, Y S Babu, Vern L Schramm
Purine nucleoside phosphorylase (PNP) is part of the human purine salvage pathway. Its deficiency triggers apoptosis of activated T-cells, making it a target for T-cell proliferative disorders. Transition-state analogues of PNP bind with picomolar (pM) dissociation constants. Tight-binding PNP inhibitors show exceptionally long lifetimes on the target enzyme. We solve the mechanism of the target residence time by comparing functional off-rates in vitro and in vivo. We report in vitro PNP-inhibitor dissociation rates (t1/2) from 3 to 31 min for seven Immucillins with dissociation constants of 115 to 6 pM...
August 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28782924/high-signal-to-background-ratio-detection-of-cancer-cells-with-activatable-strategy-based-on-target-induced-self-assembly-of-split-aptamers
#11
Baoyin Yuan, Yuqiong Sun, Qiuping Guo, Jin Huang, Xiaohai Yang, Yuanyuan Chen, Xiaohong Wen, Xiangxian Meng, Jianbo Liu, Kemin Wang
Highly sensitive detection of cancer cells with high signal-to-background ratio (SBR) is still urgently needed. Here, a self-assembling activatable probe (SAAP) based on split aptamers was developed to meet this purpose. The SAAP is formed with quenched fluorescence; only when target cells are present would the split aptamers self-assemble together and thus activate fluorescence by intramolecular and intermolecular fluorescence quenching strategies. As proof of concept, a split aptamer pair stemming from an intact aptamer, ZY11, developed by our lab was selected to construct SAAP...
August 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28777959/fast-growing-algicidal-streptomyces-sp-u3-and-its-potential-in-harmful-algal-bloom-controls
#12
Xiaoqi Yu, Guanjing Cai, Hui Wang, Zhong Hu, Wei Zheng, Xueqian Lei, Xiaoying Zhu, Yao Chen, Qiuliang Chen, Hongyan Din, Hong Xu, Yun Tian, Lijun Fu, Tianling Zheng
To find the potential algicidal microorganisms and apply them to prevent and terminate harmful algal blooms (HABs), we isolated an actinomycete U3 from Mangrove, which had a potent algicidal effect on the harmful alga Heterosigma akashiwo. It could completely lyse the algal cells by producing active compounds, which were highly sensitive to high temperature and strong alkaline, but resistant to acid. One μg/mL of crude extract of the fermentation supernatant could kill 70% of H. akashiwo cells in 3 d. Unlike most of the other known algicidal Streptomyces, U3 showed strong ability of proliferation with the algal inclusion as the nutrient source...
June 23, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28773983/an-intriguing-method-for-fabricating-arbitrarily-shaped-matreshka-hydrogels-using-a-self-healing-template
#13
Takeshi Sato, Koichiro Uto, Takao Aoyagi, Mitsuhiro Ebara
This work describes an intriguing strategy for the creation of arbitrarily shaped hydrogels utilizing a self-healing template (SHT). A SHT was loaded with a photo-crosslinkable monomer, PEG diacrylate (PEGDA), and then ultraviolet light (UV) crosslinked after first shaping. The SHT template was removed by simple washing with water, leaving behind the hydrogel in the desired physical shape. A hierarchical 3D structure such as "Matreshka" boxes were successfully prepared by simply repeating the "self-healing" and "photo-irradiation" processes...
October 25, 2016: Materials
https://www.readbyqxmd.com/read/28762895/encapsulation-of-equine-ecfcs-in-highly-uniform-injectable-hydrogel-microspheres-for-local-cell-delivery
#14
Wen J Seeto, Yuan Tian, Randolph L Winter, Fred J Caldwell, Anne A Wooldridge, Elizabeth Ann Lipke
A common challenge in cell therapy is the inability to routinely maintain survival and localization of injected therapeutic cells. Delivering cells by direct injection increases the flexibility of clinical applications, but may cause low cell viability and retention rates due to the high shear force by the needle and mechanical wash out. In this study, we present a custom-built microfluidic device that is capable of rapidly encapsulating high concentrations (10 million cells per mL) of endothelial colony forming cells (ECFCs) in poly(ethylene glycol)-fibrinogen (PF) hydrogel microspheres; resulting cell-laden microspheres are highly uniform in shape and size...
August 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28756317/sol-gel-3-glycidoxypropyltriethoxysilane-finishing-on-different-fabrics-the-role-of-precursor-concentration-and-catalyst-on-the-textile-performances-and-cytotoxic-activity
#15
M R Plutino, C Colleoni, I Donelli, G Freddi, E Guido, O Maschi, A Mezzi, G Rosace
In this paper, the influence of 3-glycidoxypropyltriethoxysilane (GPTES) based organic-inorganic coatings on the properties of treated textile fabrics was studied. All experimental results were deeply analyzed and thereafter correlated with the employed silica precursor concentration and with the presence of the BF3OEt2 (Boron trifluoride diethyl etherate), used as epoxy ring opening catalyst. SEM analysis, FT-IR spectroscopy, X-ray Photoelectron Spectroscopy (XPS), thermogravimetric analysis (TGA) and washing fastness tests of the sol-gel treated cotton fabric samples were firstly exploited in order to characterize the morphological and structural features of the achieved coatings...
July 18, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28756121/distal-airway-microbiome-is-associated-with-immunoregulatory-myeloid-cell-responses-in-lung-transplant-recipients
#16
Nirmal S Sharma, Keith M Wille, S Athira, Degui Zhi, Kenneth P Hough, Enrique Diaz-Guzman, Kui Zhang, Ranjit Kumar, Sunad Rangarajan, Peter Eipers, Yong Wang, Ritesh K Srivastava, Jose Vicente Rodriguez Dager, Mohammad Athar, Casey Morrow, Charles W Hoopes, David D Chaplin, Victor J Thannickal, Jessy S Deshane
BACKGROUND: Long-term survival of lung transplant recipients (LTRs) is limited by the occurrence of bronchiolitis obliterans syndrome (BOS). Recent evidence suggests a role for microbiome alterations in the occurrence of BOS, although the precise mechanisms are unclear. In this study we evaluated the relationship between the airway microbiome and distinct subsets of immunoregulatory myeloid-derived suppressor cells (MDSCs) in LTRs. METHODS: Bronchoalveolar lavage (BAL) and simultaneous oral wash and nasal swab samples were collected from adult LTRs...
July 15, 2017: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/28755553/remediation-of-137-cs-contaminated-concrete-using-electrokinetic-phenomena-and-ionic-salt-washes-in-nuclear-energy-contexts
#17
Andrew J Parker, Malcolm J Joyce, Colin Boxall
This work describes the first known the use of electrokinetic treatments and ionic salt washes to remediate concrete contaminated with (137)Cs. A series of experiments were performed on concrete samples, contaminated with K(+) and (137)Cs, using a bespoke migration cell and an applied electric field (60V potential gradient and current limit of 35mA). Additionally, two samples were treated with an ionic salt wash (≤400molm(-3) of KCl) alongside the electrokinetic treatment. The results show that the combined treatment produces removal efficiencies three times higher (>60%) than the electrokinetic treatment alone and that the decontamination efficiency appears to be proportional to the initial degree of contamination...
July 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28755502/an-efficient-transformation-method-for-the-bioplastic-producing-knallgas-bacterium-ralstonia-eutropha-h16
#18
Kang Lan Tee, James Grinham, Arona Martin Othusitse, Miriam Gonzalez-Villanueva, Abayomi Oluwanbe Johnson, Tuck Seng Wong
Ralstonia eutropha H16 (also known as Cupriavidus necator H16) is a Gram-negative lithoautotrophic β-proteobacterium with increasing biotechnological applications, including carbon capture and utilization, biopolymer synthesis and biofuel production. Engineering of this organism is supported by the availability of its genome sequence and suitable plasmid systems. However, the lack of a simple and robust transformation method remains a challenge as it limits both the pace and ease of engineering this organism...
July 29, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28752804/one-pot-conjugated-linoleic-acid-production-from-castor-oil-by-rhizopus-oryzae-lipase-and-resting-cells-of-lactobacillus-plantarum
#19
Abid Ali Khaskheli, Farah Naz Talpur, Aysun Cebeci Aydin, Sana Jawaid, Muhammad Ali Surhio, Hassan Imran Afridi
Conjugated linoleic acid (CLA) has attracted as novel type of fatty acids having unusual health-promoting properties such as anticarcinogenic and antiobesitic effects. The present work employed castor oil as substrate for one-pot production of CLA using washed cells of Lactobacillus plantarum (L. plantarum) and lipases as catalysts. Among the screened lipases, the lipase Rhizopus oryzae (ROL) greatly assisted resting cells to produce CLA. Mass spectral analysis of the product showed that two major isomers of CLA were produced in the reaction mixture i...
July 28, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28749266/epidemiologic-evaluation-of-elephant-endotheliotropic-herpesvirus-3b-infection-in-an-african-elephant-loxodonta-africana
#20
Ellen Bronson, Michael McClure, Jennifer Sohl, Ellen Wiedner, Sherry Cox, Erin M Latimer, Virginia R Pearson, Gary S Hayward, Angela Fuery, Paul D Ling
This epidemiologic study follows a 5-yr-old male African elephant ( Loxodonta africana ) during an episode of hemorrhagic disease (HD) due to elephant endotheliotropic herpesvirus 3B (EEHV3B) utilizing data from complete blood counts, electrophoresis and acute phase protein analysis, and polymerase chain reaction (PCR) of multiple body fluids during and after the clinical episode. The elephant presented with sudden onset of marked lethargy and inappetence followed by hypersalivation, hyperemia of the conjunctivae and focally on the tongue, and swellings on the head and ventrum...
June 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
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