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https://www.readbyqxmd.com/read/28335317/cytotoxicity-of-silver-nanoparticle-and-chitin-nanofiber-sheet-composites-caused-by-oxidative-stress
#1
Jun Kinoda, Masayuki Ishihara, Hidemi Hattori, Shingo Nakamura, Koichi Fukuda, Hidetaka Yokoe
Size-controlled spherical silver nanoparticles (<10 nm) and chitin-nanofiber sheet composites (Ag NPs/CNFS) have previously been reported to have strong antimicrobial activity in vitro. Although Ag NPs/CNFS have strong antimicrobial activity, their cytotoxicity has not been investigated. This study was performed to evaluate the effects of Ag NPs/CNFS on cytotoxicity for fibroblasts in vitro and healing delay of wound repair in vivo, focused on oxidative stress. Cytotoxic activities of Ag NPs/CNFS were investigated using a fibroblast cell proliferation assay, nitric oxide/nitrogen dioxide (NO/NO₂) measurement of the cell lysates in vitro, inhibitory effects of Ag NPs/CNFS on healing-impaired wound repair using diabetic mice in vivo, 8-hydroxy-2'-deoxyguanosine (8-OHdG) immunohistochemical staining of the skin sections, and generation of carbonyl protein in the wound was performed to evaluate cytotoxicity with oxidative stress...
October 20, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28332482/development-of-novel-silk-fibroin-polyvinyl-alcohol-sol-gel-bioactive-glass-composite-matrix-by-modified-layer-by-layer-electrospinning-method-for-bone-tissue-construct-generation
#2
B N Singh, K Pramanik
The worldwide occurrence of bone tissue related defects as well as diseases and lack of successful perpetual cure has attracted attention and accelerated exploration of composite scaffolding material with superior bioactivity, osteoinductivity and osteoconductivity properties. Among such biomaterials, silk fibroin and bioglass composite attained special emphasis to develop tissue engineered construct with a hierarchical structure ranging from nano to microscale thereby mimicking bone tissue extracellular matrix...
March 23, 2017: Biofabrication
https://www.readbyqxmd.com/read/28330474/hypercholesterolemia-downregulates-autophagy-in-the-rat-heart
#3
Zoltán Giricz, Gábor Koncsos, Tomáš Rajtík, Zoltán V Varga, Tamás Baranyai, Csaba Csonka, Adrián Szobi, Adriana Adameová, Roberta A Gottlieb, Péter Ferdinandy
BACKGROUND: We have previously shown that efficiency of ischemic conditioning is diminished in hypercholesterolemia and that autophagy is necessary for cardioprotection. However, it is unknown whether isolated hypercholesterolemia disturbs autophagy or the mammalian target of rapamycin (mTOR) pathways. Therefore, we investigated whether isolated hypercholesterolemia modulates cardiac autophagy-related pathways or programmed cell death mechanisms such as apoptosis and necroptosis in rat heart...
March 23, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28327149/release-kinetics-and-mitogenic-capacity-of-collagen-barrier-membranes-supplemented-with-secretome-of-activated-platelets-the-in-vitro-response-of-fibroblasts-of-the-periodontal-ligament-and-the-gingiva
#4
Eva-Maria Mozgan, Michael Edelmayer, Klara Janjić, Manuela Pensch, Michael B Fischer, Andreas Moritz, Hermann Agis
BACKGROUND: Platelet preparations can stimulate the healing process and have mitogenic properties. We hypothesized that collagen barrier membranes (CBM), clinically used in guided bone regeneration and guided tissue regeneration, can serve as carriers for platelet secretome. METHODS: Secretome was generated from washed platelets and unwashed platelets (washed/unwashed PSEC) and lyophilized onto CBM. Overall appearance of CBM was evaluated by scanning electron microscopy...
March 21, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28324656/investigating-the-influences-of-the-adsorbed-species-on-catalytic-activity-for-hydrogen-oxidation-reaction-in-alkaline-electrolyte
#5
Siqi Lu, Zhongbin Zhuang
Catalysts for hydrogen oxidation reaction (HOR) in alkaline electrolyte are important for anion exchange membrane fuel cells. Understanding the role of OH(-) during the HOR catalytic process in alkaline electrolyte is essential to design highly active HOR catalysts. Here, we attempt to isolate the influence of OH(-) by using surface controlled Pt based nanoparticles as the model catalysts. By comparing the HOR activity between PtNi nanoparticles and acid washed PtNi nanoparticles, which have almost the same hydrogen binding energies but much different OH binding energies, it was found that the HOR activity in alkaline electrolyte is not mainly controlled by the OH adsorption...
March 21, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28320521/differential-mirna-expression-analysis-during-late-stage-terminal-hindgut-development-in-fetal-rats
#6
Shuguang Jin, Junxiang Wang, Hong Chen, Bo Xiang
BACKGROUND: Terminal hindgut deformity is the leading digestive tract malformation, however, the etiology and pathogenesis remained unknown. To date, gene expression abnormalities were considered the primary cause of these diseases. miRNAs have been found to play an important role in regulating the expression of genes. METHODS: A total of 24 pregnant rats were randomly divided into two groups. The experimental group (n=12) received 1% ethylenethiourea (125mg/kg) by gavage on gestational day 11, while the control group (n=12) received the same volume of distilled water...
March 12, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28296254/short-term-fsh-treatment-and-sperm-maturation-a-prospective-study-in-idiopathic-infertile-men
#7
E Casamonti, S Vinci, E Serra, M G Fino, S Brilli, F Lotti, M Maggi, M E Coccia, G Forti, C Krausz
The standard FSH treatment is based on a 3 months period, after which both quantitative/qualitative improvement of sperm parameters and increased pregnancy rate were reported. In this prospective clinical trial, for the first time, we studied (i) Sperm hyaluronic acid binding capacity after highly purified FSH (hpFSH) treatment; (ii) the effect after short-term and standard treatment on this functional parameter. As secondary objective, we analyzed three SNPs on FSHβ and FSHR genes to define their potential predictive value for responsiveness...
March 15, 2017: Andrology
https://www.readbyqxmd.com/read/28291804/purification-of-cone-outer-segment-for-proteomic-analysis-on-its-membrane-proteins-in-carp-retina
#8
Takashi Fukagawa, Kazuaki Takafuji, Shuji Tachibanaki, Satoru Kawamura
Rods and cones are both photoreceptors in the retina, but they are different in many aspects including the light response characteristics and, for example, cell morphology and metabolism. These differences would be caused by differences in proteins expressed in rods and cones. To understand the molecular bases of these differences between rods and cones, one of the ways is to compare proteins expressed in rods and cones, and to find those expressed specifically or dominantly. In the present study, we are interested in proteins in the outer segment (OS), the site responsible for generation of rod- or cone-characteristic light responses and also the site showing different morphology between rods and cones...
2017: PloS One
https://www.readbyqxmd.com/read/28291798/implantable-porous-gelatin-microspheres-sustained-release-of-bfgf-and-improved-its-neuroprotective-effect-on-rats-after-spinal-cord-injury
#9
Li Lan, Fu-Rong Tian, De-Li ZhuGe, Qi-Chuan ZhuGe, Bi-Xin Shen, Bing-Hui Jin, Jian-Ping Huang, Ming-Ze Wu, Lu-Xin Fan, Ying-Zheng Zhao, He-Lin Xu
In this study, porous gelatin microspheres (GMSs) were constructed to improve the neuroprotective effect of basic fibroblast growth factor (bFGF) on spinal cord injury. GMSs were prepared by a W/O emulsion template, followed by cross-linking, washing and drying. The particle sizes and surface porosity of the blank GMSs were carefully characterized by scan electronic microscopy. The blank GMSs have a mean particle size of 35μm and theirs surface was coarse and porous. bFGF was easily encapsulated inside the bulk GMSs through diffusion along the porous channel...
2017: PloS One
https://www.readbyqxmd.com/read/28291471/chondrotoxicity-of-liposomal-bupivacaine-in-articular-chondrocytes-preliminary-findings
#10
K Aaron Shaw, Peter C Johnson, Steve Zumbrun, Augustine H Chuang, Craig D Cameron
OBJECTIVE: The chondrotoxicity of local anesthetics has been previously recognized. Recent introduction of a liposomal formulation of bupivacaine has been found to significantly improve postoperative pain control but its effect on chondrocyte viability has yet to be investigated with this new formulation. We sought to assess the in vitro chondrotoxicity of liposomal bupivacaine. METHODS: Chondrocytes were isolated from articular cartilage from fresh stifle joints and grown in culture medium...
March 2017: Military Medicine
https://www.readbyqxmd.com/read/28288928/in%C3%A2-vitro-cytotoxicity-evaluation-of-resveratrol-loaded-nanoparticles-focus-on-the-challenges-of-in%C3%A2-vitro-methodologies
#11
V Rocha, C Marques, J L Figueiredo, A R Gaio, P C Costa, J M Sousa Lobo, I F Almeida
Assessment of toxic effects is mandatory before market placement of pharmaceutical and cosmetic products. Nanotoxicology is an emerging regulatory concern and still a challenging field. Topical application of resveratrol (RSV) has been extensively studied owing to its multi-mechanistic skin anti-aging effects. Nanoencapsulation has been suggested as a promising solution to overcome RSV stability issues. In this work RSV-loaded solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) were prepared using a homogenization/sonication technique...
March 10, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28288849/whole-blood-selenium-determination-by-inductively-coupled-plasma-mass-spectrometry
#12
Dustin R Bunch, Wendy Cieslak, Sihe Wang
OBJECTIVE: To develop a sensitive method for accurately measuring whole blood selenium and determining an appropriate reference interval for the local Cleveland population. DESIGN AND METHODS: The assay was developed and validated on an inductively coupled plasma mass spectrometry (ICP-MS) with a collision cell. Whole blood trace element free EDTA tubes were used to collect samples for the reference interval study (n=50). Samples were collected after at least 8h fast from healthy adults (76% females) with ages between 19 and 64yr...
March 10, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28287384/effects-of-potassium-adsorption-filters-on-the-removal-of-ammonia-from-blood-products
#13
Hiroshi Fujita, Yoko Shiotani, Yuko Takada, Shigeko Nishimura
BACKGROUND: Although ammonia in plasma does not usually pass through the blood-brain barrier (BBB), in cases of traumatic brain injury it may do so, acting as a neurotoxin on the brain. Excess intake of ammonia should be restricted in conditions involving BBB breakdown, such as traumatic brain injury. Washing is a method to remove ammonia from blood products, but fresh-frozen plasma and albumin products cannot be washed. A potassium adsorption filter (PAF) can remove not only potassium, but also ammonia from red blood cell solutions...
March 1, 2017: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/28285055/the-role-of-routine-peritoneal-and-omental-biopsies-at-risk-reducing-salpingo-oophorectomy
#14
Heather Miller, Laura S Pipkin, Celestine Tung, Tracilyn R Hall, Ramya P Masand, Matthew L Anderson
STUDY OBJECTIVE: Assess the potential role of peritoneal and omental biopsies in women undergoing risk-reducing salpingo-oopherectomy (RRSO) for prophylactic management of hereditary breast/ovarian cancer syndromes (HBOC). DESIGN: Retrospective observational cohort DESIGN: Classification: II.1 SETTING: Academic gynecology practice PATIENTS: All women who underwent RRSO for a high risk BRCA1/2 mutation or deletion at a single institution between January, 2003 and June, 2016...
March 8, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28283714/immobilized-metal-affinity-cryogel-based-high-throughput-platform-for-screening-bioprocess-and-chromatographic-parameters-of-his6-gtpase
#15
Joyita Sarkar, Ashok Kumar
Among various tools of product monitoring, chromatography is of vital importance as it also extends to the purification of product. Immobilized metal affinity cryogel (Cu(II)-iminodiacetic acid- and Ni(II)-nitrilotriacetic acid-polyacrylamide) minicolumns (diameter 8 mm, height 4 mm, void volume 250 μl) were inserted in open-ended 96-well plate and different chromatographic parameters and bioprocess conditions were analysed. The platform was first validated with lysozyme. Optimum binding of lysozyme (∼90%) was achieved when 50 μg of protein in 20 mM Tris, pH 8...
March 10, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28283478/annexin-a2-contributes-to-lung-injury-and-fibrosis-by-augmenting-factor-xa-fibrogenic-activity
#16
Michael Schuliga, Jade Jaffar, Asres Berhan, Shenna Langenbach, Trudi Harris, David Waters, Peter Vee Sin Lee, Christopher Grainge, Glen Westall, Darryl Knight, Alastair G Stewart
In lung injury and disease, including idiopathic pulmonary fibrosis (IPF), extravascular factor X is converted into factor Xa (FXa), a coagulant protease with fibrogenic actions. Extracellular annexin A2 binds to FXa, augmenting activation of the protease activated receptor-1 (PAR-1). In this study, the contribution of annexin A2 in lung injury and fibrosis was investigated. Annexin A2 immunoreactivity was observed in regions of fibrosis, including associated with fibroblasts in lung tissue of IPF patients...
March 10, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28281330/basophil-activation-test-implementation-and-standardization-between-systems-and-between-instruments
#17
Anne-Emmanuelle Depince-Berger, Khaled Sidi-Yahya, Mohammed Jeraiby, Claude Lambert
The basophil activation test (BAT) is a good ex vivo alternative for measuring hypersensitivity to an allergen in sensitized patients but still lacks standardization. In this present study, we have implemented one of the systems and proposed inter-systems, inter-instrument standardization. Our method for basophil activation and labeling on whole blood: EDTA in one step using BasoflowEx(®) and FlowCast(®) . Setup on Navios and fluorescence targets converted to set up FACSCanto™ instrument. Our results: 1) A CD203c/CD63 (BasoflowEx) method was adapted for EDTA samples and simplified...
March 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28280761/comparison-of-candida-albicans-adherence-to-conventional-acrylic-denture-base-materials-and-injection-molding-acrylic-materials
#18
Masoomeh Aslanimehr, Shirin Rezvani, Ali Mahmoudi, Najmeh Moosavi
STATEMENT OF THE PROBLEM: Candida species are believed to play an important role in initiation and progression of denture stomatitis. The type of the denture material also influences the adhesion of candida and development of stomatitis. PURPOSE: The aim of this study was comparing the adherence of candida albicans to the conventional and injection molding acrylic denture base materials. MATERIALS AND METHOD: Twenty injection molding and 20 conventional pressure pack acrylic discs (10×10×2 mm) were prepared according to their manufacturer's instructions...
March 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28280410/lamotrigine-an-antiepileptic-drug-inhibits-5-ht3-receptor-currents-in-ncb-20-neuroblastoma-cells
#19
Ki Jung Kim, Seung Hyun Jeun, Ki-Wug Sung
Lamotrigine is an antiepileptic drug widely used to treat epileptic seizures. Using whole-cell voltage clamp recordings in combination with a fast drug application approach, we investigated the effects of lamotrigine on 5-hydroxytryptamine (5-HT)3 receptors in NCB-20 neuroblastoma cells. Co-application of lamotrigine (1~300 µM) resulted in a concentration-dependent reduction in peak amplitude of currents induced by 3 µM of 5-HT for an IC50 value of 28.2±3.6 µM with a Hill coefficient of 1.2±0.1. These peak amplitude decreases were accompanied by the rise slope reduction...
March 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28274532/utility-of-cell-blocks-obtained-by-catheter-aspiration-via-a-guide-sheath-during-endobronchial-ultrasonography
#20
Nobuko Hazeki, Motoko Tachihara, Ryuko Tsukamoto, Shuntaro Tokunaga, Daisuke Tamura, Haruko Shinke, Kazuyuki Kobayashi, Yasuhiro Sakai, Yoshihiro Nishimura
BACKGROUND: The demand for adequate tissue samples for both morphological assessment and molecular studies on lung cancer treatment has increased. The aim of this study was to evaluate whether cell blocks (CBs) prepared from endobronchial ultrasonography with guide sheath (EBUS-GS) rinsing following catheter aspiration provide additional information. METHODS: We produced CBs from rinse fluid obtained from washing the inside of the sheath with saline after conventional EBUS-GS between May 2012 and April 2013...
March 2017: Respiratory Investigation
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