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DNA nanotechnology

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https://www.readbyqxmd.com/read/28918263/dna-nanotechnology-based-composite-type-gold-nanoparticle-immunostimulatory-dna-hydrogel-for-tumor-photothermal-immunotherapy
#1
Tomoya Yata, Yuki Takahashi, Mengmeng Tan, Hirotaka Nakatsuji, Shozo Ohtsuki, Tatsuya Murakami, Hiroshi Imahori, Yuka Umeki, Tomoki Shiomi, Yoshinobu Takakura, Makiya Nishikawa
Success of tumor photothermal immunotherapy requires a system that induces heat stress in cancer cells and enhances strong anti-tumor immune responses. Here, we designed a composite-type immunostimulatory DNA hydrogel consisting of a hexapod-like structured DNA (hexapodna) with CpG sequences and gold nanoparticles. Mixing of the properly designed hexapodna and oligodeoxynucleotide-modified gold nanoparticles resulted in the formation of composite-type gold nanoparticle-DNA hydrogels. Laser irradiation of the hydrogel resulted in the release of hexapodna, which efficiently stimulated immune cells to release proinflammatory cytokines...
September 9, 2017: Biomaterials
https://www.readbyqxmd.com/read/28899817/acute-oral-toxicity-study-of-magnesium-oxide-nanoparticles-and-microparticles-in-female-albino-wistar-rats
#2
Bhanuramya Mangalampalli, Naresh Dumala, Paramjit Grover
Advancements in nanotechnology have led to the development of the nanomedicine, which involves nanodevices for diagnostic and therapeutic purposes. A key requirement for the successful use of the nanoparticles (NPs) in biomedical applications is their good dispensability, colloidal stability in biological media, internalization efficiency, and low toxicity. Therefore, toxicological profiling is necessary to understand the mechanism of NPs and microparticles (MPs). MgO NPs have attracted wide scientific interest due to ease of synthesis, chemical stability and unique properties...
September 9, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28893984/nanoswitch-linked-immunosorbent-assay-nlisa-for-fast-sensitive-and-specific-protein-detection
#3
Clinton H Hansen, Darren Yang, Mounir A Koussa, Wesley P Wong
Protein detection and quantification play critical roles in both basic research and clinical practice. Current detection platforms range from the widely used ELISA to more sophisticated, and more expensive, approaches such as digital ELISA. Despite advances, there remains a need for a method that combines the simplicity and cost-effectiveness of ELISA with the sensitivity and speed of modern approaches in a format suitable for both laboratory and rapid, point-of-care applications. Building on recent developments in DNA structural nanotechnology, we introduce the nanoswitch-linked immunosorbent assay (NLISA), a detection platform based on easily constructed DNA nanodevices that change conformation upon binding to a target protein with the results read out by gel electrophoresis...
September 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28888402/investigating-the-potential-of-multiwalled-carbon-nanotubes-based-zinc-nanocomposite-as-a-recognition-interface-towards-plant-pathogen-detection
#4
Muhammad Ali Tahir, Sadaf Hameed, Anam Munawar, Imran Amin, Shahid Mansoor, Waheed S Khan, Sadia Zafar Bajwa
The emergence of nanotechnology has opened new horizons for constructing efficient recognition interfaces. This is the first report where the potential of a multiwalled carbon nanotube based zinc nanocomposite (MWCNTs-Zn NPs) investigated for the detection of an agricultural pathogen i.e. Chili leaf curl betasatellite (ChLCB). Atomic force microscope analyses revealed the presence of multiwalled carbon nanotubes (MWCNTs) having a diameter of 50-100nm with zinc nanoparticles (Zn-NPs) of 25-500nm. In this system, these bunches of Zn-NPs anchored along the whole lengths of MWCNTs were used for the immobilization of probe DNA strands...
September 6, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28887655/freezing-shortens-the-lifetime-of-dna-molecules-under-tension
#5
Wei-Ju Chung, Yujia Cui, Chi-Shuo Chen, Wesley H Wei, Rong-Shing Chang, Wun-Yi Shu, Ian C Hsu
DNA samples are commonly frozen for storage. However, freezing can compromise the integrity of DNA molecules. Considering the wide applications of DNA molecules in nanotechnology, changes to DNA integrity at the molecular level may cause undesirable outcomes. However, the effects of freezing on DNA integrity have not been fully explored. To investigate the impact of freezing on DNA integrity, samples of frozen and non-frozen bacteriophage lambda DNA were studied using optical tweezers. Tension (5-35 pN) was applied to DNA molecules to mimic mechanical interactions between DNA and other biomolecules...
September 8, 2017: Journal of Biological Physics
https://www.readbyqxmd.com/read/28866510/multiscale-visualization-and-scale-adaptive-modification-of-dna-nanostructures
#6
Haichao Miao, Elisa De Llano, Johannes Sorger, Yasaman Ahmadi, Tadija Kekic, Tobias Isenberg, M Eduard Groller, Ivan Barisic, Ivan Viola
We present an approach to represent DNA nanostructures in varying forms of semantic abstraction, describe ways to smoothly transition between them, and thus create a continuous multiscale visualization and interaction space for applications in DNA nanotechnology. This new way of observing, interacting with, and creating DNA nanostructures enables domain experts to approach their work in any of the semantic abstraction levels, supporting both low-level manipulations and high-level visualization and modifications...
August 29, 2017: IEEE Transactions on Visualization and Computer Graphics
https://www.readbyqxmd.com/read/28866281/integrated-assessment-of-toxic-effects-of-maghemite-%C3%AE-fe2o3-nanoparticles-in-zebrafish
#7
Rolando A R Villacis, José S Filho, Benjamin Piña, Ricardo B Azevedo, Aline Pic-Taylor, Juliana F Mazzeu, Cesar K Grisolia
The increasing use of nanotechnology in the last decade has raised concerns about the impact of nanoparticles in the environment. In particular, the potential harmful effects of iron oxide nanoparticles (IONPs) in aquatic organisms have been poorly addressed. We analyze here the toxic effects induced by IONPs in zebrafish using a combination of classical (genotoxicity, oxidative stress) and molecular (transcriptomic) methodologies. Adult animals were exposed for 96h to five sub-lethal IONP concentrations, ranging from 4...
August 8, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28858402/fullerene-cluster-assisted-self-assembly-of-short-dna-strands-into-semiconducting-nanowires
#8
Sandeepa Kulala Vittala, Sajena Kanangat Saraswathi, Joshy Joseph
Programmable, hierarchical assembly of DNA nanostructures with the precise organization of functional components have been demonstrated previously with tiled assembly and DNA Origami. However, building organized nanostructures with random oligonucleotide strands remains as an elusive problem. Herein, we describe a simple and general strategy in which nanoclusters of a fullerene derivative act as stapler motifs in bringing ordered nanoscale assembly of short oligonucleotide duplexes into micrometer-sized nanowires...
August 31, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28852183/temperature-and-pressure-limits-of-guanosine-monophosphate-self-assemblies
#9
Mimi Gao, Balasubramanian Harish, Melanie Berghaus, Rana Seymen, Loana Arns, Scott A McCallum, Catherine A Royer, Roland Winter
Guanosine monophosphate, among the nucleotides, has the unique property to self-associate and form nanoscale cylinders consisting of hydrogen-bonded G-quartet disks, which are stacked on top of one another. Such self-assemblies describe not only the basic structural motif of G-quadruplexes formed by, e.g., telomeric DNA sequences, but are also interesting targets for supramolecular chemistry and nanotechnology. The G-quartet stacks serve as an excellent model to understand the fundamentals of their molecular self-association and to unveil their application spectrum...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28843597/molecular-dynamics-simulations-and-applications-in-computational-toxicology-and-nanotoxicology
#10
Chandrabose Selvaraj, Sugunadevi Sakkiah, Weida Tong, Huixiao Hong
Nanotoxicology studies toxicity of nanomaterials and has been widely applied in biomedical researches to explore toxicity of various biological systems. Investigating biological systems through in vivo and in vitro methods is expensive and time taking. Therefore, computational toxicology, a multi-discipline field that utilizes computational power and algorithms to examine toxicology of biological systems, has gained attractions to scientists. Molecular dynamics (MD) simulations of biomolecules such as proteins and DNA are popular for understanding of interactions between biological systems and chemicals in computational toxicology...
August 24, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28842529/structure-of-the-holliday-junction-applications-beyond-recombination
#11
REVIEW
P Shing Ho
The Holliday junction (HJ) is an essential element in recombination and related mechanisms. The structure of this four-stranded DNA assembly, which is now well-defined alone and in complex with proteins, has led to its applications in areas well outside of molecular recombination, including nanotechnology and biophysics. This minireview explores some interesting recent research on the HJ, as it has been adapted to design regular two- or three-dimensional lattices for crystal engineering, and more complex systems through DNA origami...
August 24, 2017: Biochemical Society Transactions
https://www.readbyqxmd.com/read/28835943/dna-tetrahedron-nanostructures-for-biological-applications-biosensors-and-drug-delivery
#12
REVIEW
Nuli Xie, Shiyuan Liu, Xiaohai Yang, Xiaoxiao He, Jin Huang, Kemin Wang
With the rapid development of DNA nanotechnology, various DNA nanostructures with different shapes and sizes have been self-assembled using "bottom-up" fabrication strategies and applied to a wide range of fields such as biosensors, drug delivery and tools for molecular biology. As a classical and simple polyhedron, DNA tetrahedron can be easily synthesised by a one-step assembly. Due to the excellent biocompatibility and cellular permeability, it provides a universal and promising platform to construct a series of biosensors and drug delivery systems for living cells studies...
August 24, 2017: Analyst
https://www.readbyqxmd.com/read/28833684/metal-mediated-base-pairs-from-characterization-to-application
#13
Biswarup Jash, Jens Müller
The investigation of metal-mediated base pairs and the development of their applications represent a prominent area of research at the border of bioinorganic chemistry and supramolecular coordination chemistry. In metal-mediated base pairs, the complementary nucleobases in a nucleic acid duplex are connected via coordinate bonds to an embedded metal ion rather than by hydrogen bonds. Because metal-mediated base pairs facilitate a site-specific introduction of metal-based functionality into nucleic acids, they are ideally suited for use in DNA nanotechnology...
August 23, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28833683/self-assembly-of-heterogeneously-shaped-nanoparticles-into-plasmonic-metamolecules-on-dna-origami
#14
Wenyan Liu, Ling Li, Shuo Yang, Jie Gao, Risheng Wang
Fabrication of plasmonic metamolecules (PMs) with rationally designed complexity is one of the major goals of nanotechnology. Most self-assembled PMs, however, have been constructed using single-component systems. The corresponding plasmonic assemblies still suffer from the lack of complexity, which is required to achieve a high degree of functionality. Here we report a general applicable strategy that can realize a series of high-ordered hetero-PMs using bottom-up DNA self-assembly. DNA functionalized differently-shaped nanoparticles were deliberately arranged in prescribed positions on 3D triangular DNA origami frames to form various hetero-PMs...
August 23, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28828981/the-primary-mechanism-of-cellular-internalization-for-a-short-cellpenetrating-peptide-as-a-nano-scale-delivery-system
#15
Betty R Liu, Yue-Wern Huang, Mallikarjuna Korivi, Shih-Yen Lo, Robert S Aronstam, Han-Jung Lee
BACKGROUND: Development of effective drug delivery systems (DDS) is a critical issue in health care and medicine. Advances in molecular biology and nanotechnology have allowed the introduction of nanomaterial-based drug delivery systems. Cell-penetrating peptides (CPPs) can form the basis of drug delivery systems by virtue of their ability to support the transport of cargoes into the cell. Potential cargoes include proteins, DNA, RNA, liposomes, and nanomaterials. These cargoes generally retain their bioactivities upon entering cells...
August 22, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28817122/single-cell-mechanogenetics-using-monovalent-magnetoplasmonic-nanoparticles
#16
Ji-Wook Kim, Daeha Seo, Jung-Uk Lee, Kaden M Southard, Yongjun Lim, Daehyun Kim, Zev J Gartner, Young-Wook Jun, Jinwoo Cheon
Spatiotemporal interrogation of signal transduction at the single-cell level is necessary to answer a host of important biological questions. This protocol describes a nanotechnology-based single-cell and single-molecule perturbation tool, termed mechanogenetics, that enables precise spatial and mechanical control over genetically encoded cell-surface receptors in live cells. The key components of this tool are a magnetoplasmonic nanoparticle (MPN) actuator that delivers defined spatial and mechanical cues to receptors through target-specific one-to-one engagement and a micromagnetic tweezers (μMT) that remotely controls the magnitude of force exerted on a single MPN...
September 2017: Nature Protocols
https://www.readbyqxmd.com/read/28816314/pt-supraparticles-with-controllable-dna-valences-for-programmed-nanoassembly
#17
Gaoli Chen, Song Wang, Lei Song, Xiaojun Song, Zhaoxiang Deng
Supraparticles are self-limiting nanoparticle ensembles with attractive properties from their unique hierarchical (primary and secondary) structures. Aiming at relieving the bottleneck of the very limited material building blocks in DNA nanotechnology, we herein demonstrate Pt-based supraparticles as catalytic materials for valence-controllable and high density DNA functionalizations toward DNA-programmed nanoassembly.
August 29, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28809101/a-microsphere-supported-lipid-bilayer-platform-for-dna-reactions-on-a-fluid-surface
#18
Aurora Fabry-Wood, Madalyn E Fetrow, Carl W Brown, Nicholas A Baker, Nadiezda Fernandez Oropeza, Andrew P Shreve, Gabriel A Montaño, Darko Stefanovic, Matthew R Lakin, Steven W Graves
We report a versatile microsphere-supported lipid bilayer system that can serve as a general-purpose platform for implementing DNA nanotechnologies on a fluid surface. To demonstrate our platform, we implemented both toehold-mediated strand displacement (TMSD) and DNAzyme reactions, which are typically performed in solution and which are the cornerstone of DNA-based molecular logic and dynamic DNA nanotechnology, on the surface. We functionalized microspheres bearing supported lipid bilayers (μSLBs) with membrane-bound nucleic acid components...
August 24, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28806061/construction-of-a-polyhedral-dna-12-arm-junction-for-self-assembly-of-wireframe-dna-lattices
#19
Ilenia Manuguerra, Guido Grossi, Rasmus P Thomsen, Jeppe Lyngsø, Jan S Pedersen, Jørgen Kjems, Ebbe S Andersen, Kurt V Gothelf
A variety of different tiles for the construction of DNA lattices have been developed since the structural DNA nanotechnology field was born. The majority of these are designed for the realization of close-packed structures, where DNA helices are arranged in parallel and tiles are connected through sticky ends. Assembly of such structures requires the use of cation-rich buffers to minimize repulsion between parallel helices, which poses limits to the application of DNA nanostructures. Wireframe structures, on the other hand, are less susceptible to salt concentration, but the assembly of wireframe lattices is limited by the availability of tiles and motifs...
August 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28805877/a-dna-bipedal-nanowalker-with-a-piston-like-expulsion-stroke
#20
Q Y Yeo, I Y Loh, S R Tee, Y H Chiang, J Cheng, M H Liu, Z S Wang
Artificial molecular walkers beyond burn-bridge designs are important for nanotechnology, but their systematic development remains difficult. Herein, we have reported a new rationally designed DNA walker-track system and experimentally verified a previously proposed general expulsion regime for implementing non-burn-bridge nanowalkers. The DNA walker has an optically powered engine motif that reversibly extends and contracts the walker via a quadruplex-duplex conformational change. The walker's extension is an energy-absorbing and force-generating process, which drives the walker's leg dissociation off-track in a piston-like expulsion stroke...
August 24, 2017: Nanoscale
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