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https://www.readbyqxmd.com/read/28073080/impact-of-environmental-factors-on-the-culturability-and-viability-of-listeria-monocytogenes-under-conditions-encountered-in-food-processing-plants
#1
Anaïs Overney, Joséphine Jacques-André-Coquin, Patricia Ng, Brigitte Carpentier, Laurent Guillier, Olivier Firmesse
The ability of Listeria monocytogenes to adhere to and persist on surfaces for months or even years may be responsible for its transmission from contaminated surfaces to food products. Hence the necessity to find effective means to prevent the establishment of L. monocytogenes in food processing environments. The aim of this study was to assess, through a fractional experimental design, the environmental factors that could affect the survival of L. monocytogenes cells on surfaces to thereby prevent the persistence of this pathogen in conditions mimicking those encountered in food processing plants: culture with smoked salmon juice or meat exudate, use of two materials with different hygiene status, biofilm of L...
December 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28058525/the-visual-assessment-of-broth-cultures-for-tissue-bank-samples
#2
Kerry Varettas
The bioburden screening process of allograft musculoskeletal tissue samples received at the South Eastern Area Laboratory Services includes the routine use of solid agar and cooked meat (CM) broth media. CM has been routinely sub-cultured onto solid agar plates after aerobic incubation at 35 °C. This study will evaluate whether a visual assessment of CM can replace sub-culture by an in vitro inoculation and a prospective study. Eight challenge organisms were serially diluted and inoculated into CM. The average inoculum of 0...
January 5, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28043380/survival-of-toxoplasma-gondii-tachyzoites-in-simulated-gastric-fluid-and-cow-s-milk
#3
Martin Koethe, Carolin Schade, Karsten Fehlhaber, Martina Ludewig
The protozoan parasite Toxoplasma gondii is one of the most important food-related pathogens worldwide. Besides contact to oocysts or ingestion of tissue cysts mainly by consumption of raw or undercooked meat from infected animals, raw milk is considered to be a risk factor and possible route of transmission for tachyzoites. This stage of the parasite is usually very sensitive to acidic pH and, therefore, considered unlikely to survive stomach passage. However, tachyzoites were shown to survive for several days in milk and there are also reports on transmission of toxoplasmosis via milk...
January 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28040506/meat-and-masculinity-in-the-norwegian-armed-forces
#4
Charlotte Lilleby Kildal, Karen Victoria Lykke Syse
In 2013, the Norwegian Armed Forces decided to introduce a meat reduction scheme in the military mess halls, due to both health reasons and environmental concerns. This article explores Norwegian soldiers' reactions to the introduction of Meat free Monday, and their attitudes towards reducing meat consumption. As of yet, Meat free Monday has not been implemented due to both structural and contextual challenges. We found significant barriers preventing the military from implementing Meat free Monday. The main reason behind the resistance to reduce meat consumption among Norwegian soldiers was meat's associations with masculinity, power and comfort...
December 28, 2016: Appetite
https://www.readbyqxmd.com/read/28034292/the-effect-of-pullulan-on-the-growth-and-acidifying-activity-of-selected-stool-microflora-of-human
#5
Anna Chlebowska-Śmigiel, Małgorzata Gniewosz, Marek Kieliszek, Anna Bzducha-Wróbel
BACKGROUND: Pullulan is a microbial polysaccharide of low energy value, which can component of low-calorie foods and in dietary snacks for diabetics. The objective of the study was to determine the effect of pullulan on the growth and fermentation activity of selected human intestinal bacteria. METHODS: Commercial pullulan purchased from Focubase (China) of a molecular weight of 100,000 Da constituted as experimental material. Food grade pullulan 99% purity. Two control media were prepared: the first standard RCM composed of (g/100 ml): 0...
December 29, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28028960/detection-of-salmonella-in-foods-using-a-reference-pn-iso-method-and-an-alternative-method-based-on-loop-mediated-isothermal-amplification-coupled-with-bioluminescence
#6
Jolanta Sarowska, Magdalena Frej-Mądrzak, Agnieszka Jama-Kmiecik, Anna Kilian, Dorota Teryks-Wołyniec, Irena Choroszy-Król
BACKGROUND: Salmonella, one of the primary indicators of food safety, is a common cause of food poisoning of an epidemic nature around the world. These microorganisms can colonize the gastrointestinal tract of both people and animals, and next contaminate not only eggs, milk, meat and dairy products, but also vegetables, fruit, grains and even spices. OBJECTIVES: The aim of this study was to analyze the frequency of detection of Salmonella spp. in food samples using a reference PN-ISO method and an alternative method based on loop-mediated isothermal amplification (LAMP) coupled with bioluminescence...
September 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/27988853/status-of-bovine-tuberculosis-and-its-zoonotic-implications-in-borana-zone-southern-ethiopia
#7
Ararsa Duguma, Shubisa Abera, Wubishet Zewdie, Dinaol Belina, Godana Haro
A cross-sectional study was carried out from April 2015 to June 2016 to estimate the status of bovine tuberculosis (BTB), assessment of community's current knowledge, and zoonotic importance on this disease in Borana zone, southern Ethiopia. In this study, comparative intradermal tuberculin (CIDT) test, structured questionnaires, and retrospective data were used, while the result indicated 3.8% prevalence at individual animal level with 5.6% (31/554) of doubtful reactors. Among related risk factors included, old animals were significantly infected by BTB than young one (χ (2) = 32...
December 17, 2016: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27986726/detection-of-salmonella-in-meat-in-less-than-5-hours-by-a-low-cost-and-non-complex-sample-preparation-method
#8
M S R Fachmann, C Löfström, J Hoorfar, F Hansen, J Christensen, S Mansdal, M H Josefsen
: Salmonella is recognised as one of the most important foodborne bacteria, and has a wide health and socioeconomical impact worldwide. Fresh pork meat is one of the main sources of Salmonella and efficient and fast methods for detection are therefore necessary. Current methods for Salmonella detection in fresh meat usually include >16 h of culture enrichment, in few cases < 12 h, thus requiring at least two working shifts. Here, we report a rapid (< 5 h) and high throughput method, for screening of Salmonella in samples from fresh pork meat, consisting of a 3-h enrichment in standard buffered peptone water, and a real-time PCR compatible sample preparation method, based on filtration, centrifugation, and enzymatic digestion, followed by fast cycling real-time PCR detection...
December 16, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27980380/prevalence-and-antibiotic-susceptibility-profiles-of-listeria-monocytogenes-contamination-of-chicken-flocks-and-meat-in-oyo-state-south-western-nigeria-public-health-implications
#9
REVIEW
O O Ishola, J I Mosugu, H K Adesokan
INTRODUCTION: Food contamination with Listeria monocytogenes is on the increase posing threats to public health with growing trends in food products recalls due to suspected Listeria contamination. METHODS: We conducted a cross-sectional study to determine the prevalence and antibiotic susceptibility profiles of Listeria monocytogenes (Lm) among 71 randomly selected poultry farms in Oyo State, Nigeria. A total of 450 samples comprising cloacal swabs (426) and randomly selected dressed chicken meat (24) were cultured for Lm isolation using BrillianceTM Selective Listeria Agar with antibiotics and microbial load count with Nutrient Agar...
September 2016: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/27974596/improving-diet-sustainability-through-evolution-of-food-choices-review-of-epidemiological-studies-on-the-environmental-impact-of-diets
#10
Marlène Perignon, Florent Vieux, Louis-Georges Soler, Gabriel Masset, Nicole Darmon
The Food and Agriculture Organization defines sustainable diets as nutritionally adequate, safe, healthy, culturally acceptable, economically affordable diets that have little environmental impact. This review summarizes the studies assessing, at the individual level, both the environmental impact and the nutritional quality or healthiness of self-selected diets. Reductions in meat consumption and energy intake were identified as primary factors for reducing diet-related greenhouse gas emissions. The choice of foods to replace meat, however, was crucial, with some isocaloric substitutions possibly increasing total diet greenhouse gas emissions...
January 2017: Nutrition Reviews
https://www.readbyqxmd.com/read/27931747/genetic-diversity-and-population-structure-of-food-borne-staphylococcus-carnosus-strains
#11
Anne Bückle Née Müller, Markus Kranz, Herbert Schmidt, Agnes Weiss
The species Staphylococcus carnosus is a non-pathogenic representative of the coagulase negative staphylococci. Specific strains are applied as meat starter cultures. The species consists of two subspecies, S. carnosus ssp. carnosus and S. carnosus ssp. utilis. In order to place S. carnosus strains, characterized in former studies, in a genetic background that allows a typing of candidates for starter cultures, a Multilocus Sequence Typing (MLST) scheme was developed. Internal fragments of the genes tpiA, xprT, dat, gmk, glpK, narG, cstA, encoding triosephosphate isomerase, xanthine phosphoribosyltransferase, d-amino acid aminotransferase, guanylate kinase, glycerol kinase, the α-chain of the respiratory nitrate reductase, and a carbon starvation protein where chosen...
November 30, 2016: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/27917968/innovations-in-food-preservation-in-pastoral-zones
#12
C Jans, D W Mulwa Kaindi, L Meile
Food preservation makes a significant contribution to food security and food safety in pastoral communities with limited access to external food sources. Raw materials are preserved by heating, drying, smoking, pickling, salting, curing or fermentation with microorganisms. This article describes preservation techniques in the pastoral context, targeting the major dietary components of milk, meat and cereals; related health risks; and potential innovations for food preservation. Sustainable elimination of pathogenic microorganisms, preventing re-contamination, sporulation and the growth of zoonotic and foodborne microorganisms, is necessary to enhance food safety and ensure food security by reducing post-harvest losses and food waste...
November 2016: Revue Scientifique et Technique
https://www.readbyqxmd.com/read/27889163/differential-detection-of-pathogenic-yersinia-spp-by-fluorescence-in-situ-hybridization
#13
Alexander Rohde, Jens Andre Hammerl, Bernd Appel, Ralf Dieckmann, Sascha Al Dahouk
Yersinia enterocolitica, Y. pseudotuberculosis and Y. pestis are pathogens of major medical importance, which are responsible for a considerable number of infections every year. The detection of these species still relies on cultural methods, which are slow, labour intensive and often hampered by the presence of high amounts of accompanying flora. In this study, fluorescence in situ hybridization (FISH) was used to develop a fast, sensitive and reliable alternative to detect viable bacteria in food. For this purpose, highly specific probes targeting the 16S and 23S ribosomal RNA were employed to differentially detect each of the three species...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27872721/the-role-of-fibroblast-growth-factor-receptor-2-fgfr2-in-differentiation-of-bovine-spermatogonial-stem-cells-scc
#14
Rahem Khoshbakht, Mohammad Tabatabaei, Saeid Hoseinzadeh, Mojtaba Mojtaba, Hesamaddin Shirzad Aski, Enayat Berizi
Although poultry meat is considered as the main source for human Campylobacter infections, there is limited information about non-poultry sources. The present study was aimed to investigate the prevalence and the antibiotic resistance of thermophilic Campylobacter spp. in fecal samples of the cattle and sheep in Shiraz, Iran. A total of 302 fecal samples were obtained from clinically healthy, slaughtered cattle and sheep from Shiraz slaughterhouse. The animals were clinically healthy before being slaughtered...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27871797/direct-pcr-a-rapid-method-for-multiplexed-detection-of-different-serotypes-of-salmonella-in-enriched-pork-meat-samples
#15
Wai Hoe Chin, Yi Sun, Jonas Høgberg, Than Linh Quyen, Pia Engelsmann, Anders Wolff, Dang Duong Bang
Salmonellosis, an infectious disease caused by Salmonella spp., is one of the most common foodborne diseases. Isolation and identification of Salmonella by conventional bacterial culture method is time consuming. In response to the demand for rapid on line or at site detection of pathogens, in this study, we developed a multiplex Direct PCR method for rapid detection of different Salmonella serotypes directly from pork meat samples without any DNA purification steps. An inhibitor-resistant Phusion Pfu DNA polymerase was used to overcome PCR inhibition...
November 18, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27871121/high-throughput-sequencing-of-viable-microbial-communities-in-raw-pork-subjected-to-a-fast-cooling-process
#16
Chao Yang, You Che, Yan Qi, Peixin Liang, Cunjiang Song
This study aimed to investigate the effect of the fast cooling process on the microbiological community in chilled fresh pork during storage. We established a culture-independent method to study viable microbes in raw pork. Tray-packaged fresh pork and chilled fresh pork were completely spoiled after 18 and 49 d in aseptic bags at 4 °C, respectively. 16S/18S ribosomal RNAs were reverse transcribed to cDNA to characterize the activity of viable bacteria/fungi in the 2 types of pork. Both cDNA and total DNA were analyzed by high-throughput sequencing, which revealed that viable Bacteroides sp...
January 2017: Journal of Food Science
https://www.readbyqxmd.com/read/27861952/a-possible-approach-to-assess-acidification-of-meat-starter-cultures-a-case-study-from-some-wild-strains-of-lactobacillus-plantarum
#17
Barbara Speranza, Antonio Bevilacqua, Maria Rosaria Corbo, Milena Sinigaglia
BACKGROUND: The performances of four autochthonous isolates of Lactobacillus plantarum were assessed to study the most important variables acting on acidification and to propose a possible step-by-step approach for the validation at laboratory scale. This main topic was addressed through three intermediate steps: (1) evaluation of acidification in liquid and solid media, as a function of salt, nitrites, nitrates, lactose, pepper and temperature; (2) assessing acidification in a pork-meat preparation; and (3) designing a protocol to improve the performances at sub-optimal temperatures...
November 12, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27860343/isolation-of-campylobacter-spp-from-client-owned-dogs-and-cats-and-retail-raw-meat-pet-food-in-the-manawatu-new-zealand
#18
K Bojanić, A C Midwinter, J C Marshall, L E Rogers, P J Biggs, E Acke
Campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. The disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. The prevalence of Campylobacter spp. in dogs and cats varies widely, and data on New Zealand pets are limited. This study aimed to investigate the prevalence of Campylobacter spp. in dogs, cats and retail raw meat pet food products in New Zealand and to characterize Campylobacter jejuni isolates using multilocus sequence typing (MLST)...
November 12, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/27859340/amino-acid-profile-and-sensory-characteristics-of-dry-fermented-pork-loins-produced-with-a-mixture-of-probiotic-starter-cultures
#19
Katarzyna Neffe-Skocińska, Anna Okoń, Danuta Kołożyn-Krajewska, Zbigniew Dolatowski
BACKGROUND: Proteolysis is a biochemical process in dry-aged meat products where proteins are metabolized and broken down to polypeptides, peptides, and free amino acids. In the literature it is reported that an appropriate choice of probiotic starter culture limits proteolytic changes in dry-fermented meat products. In this study the combined effect of a mixture of probiotic starter cultures on the free amino acid profile, total count of lactic acid bacteria, and the sensory quality of dry-aged pork loins after fermentation and after storing the vacuum-packed samples was evaluated...
November 9, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27853716/contamination-of-bovine-sheep-and-goat-meat-with-brucella-spp
#20
Francesco Casalinuovo, Lucia Ciambrone, Antonio Cacia, Paola Rippa
A study was conducted in order to evaluate the contamination by Brucella spp. of meat from animals slaughtered because they had resulted positive for brucellosis at some time during their life. After slaughter and before delivery to market outlets, swab samples were taken from 307 carcasses of infected animals: 40 cattle, 60 sheep and 207 goats. The swabs were subsequently analysed by means of polymerase chain reaction (PCR) tests. In addition, bacteriological tests were carried out on the lymph nodes and internal organs of the same animals...
June 3, 2016: Italian Journal of Food Safety
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