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Cultured meat

Mayowa Agunbiade, Carolina Pohl, Omotayo Ashafa
A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank as Streptomyces platensis with accession number FJ 486385.1. Culture conditions for optimal production of the bioflocculant included glucose as a sole carbon source, resulting in flocculating activity of 90%. Other optimal conditions included: peptone as nitrogen source; presence of Mg2+ as cations and inoculum size of 1...
April 5, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Sirirat Wachiralurpan, Thayat Sriyapai, Supatra Areekit, Pichapak Sriyapai, Suphitcha Augkarawaritsawong, Somchai Santiwatanakul, Kosum Chansiri
Listeria monocytogenes is a major foodborne pathogen of global health concern. Herein, the rapid diagnosis of L. monocytogenes has been achieved using loop-mediated isothermal amplification (LAMP) based on the phosphatidylcholine-phospholipase C gene ( plcB ). Colorimetric detection was then performed through the formation of DNA concatemers and a gold nanoparticle/DNA probe complex (GNP/DNA probe). The overall detection process was accomplished within approximately 1 h with no need for complicated equipment...
2018: Frontiers in Chemistry
Fumio Watanabe, Tomohiro Bito
Cobalamin, also known as the red-colored vitamin B12 , is found in animal-based foods such as meat, milk, and fish. Various cobalamin compounds are extracted from foods and converted into cyanocobalamin, which is most stable, to be analyzed by various methods. Traditionally, the cobalamin content of foods is determined by microbiological assay with Lactobacillus delbrueckii subsp. lactis American Type Culture Collection 7830. However, this lactic acid bacterium can substitute deoxyribosides or deoxynucleotides (known as an alkali-resistant factor) for cobalamin...
April 18, 2018: Journal of AOAC International
Rakhi Chakraborty, Swarnendu Roy
The Himalayas have provided shelter to the various communities for thousands of years and have remained the cradle for the origin of diverse cultures and traditions. The Himalayan belt is rich in biodiversity and have ushered mankind with numerous gifts for survival and existence. The art of pickling is believed to have developed independently among the different communities of this region. In this region, the main meal is supplemented with pickles or achar that not only adds flavour, but also enhances the value of the meal and often comes with inherent health benefits...
May 2018: Journal of Food Science and Technology
Yao Yao, Xianghui Chen, Shengzheng Wu, Liang Guo, Hao Zhang, Qiao Zhu, Jie Tang, Fuxin Luan, Yali Zhao, Faqin Lv, Yao He
Background: Thyroid nodules (TNs) are common thyroid lesions in older population. Few studies have focused on the prevalence of TNs and their relationship to lifestyle characteristics and dietary habits in centenarians. The current study aimed at determining the prevalence of TNs in Chinese centenarians by using high-resolution ultrasound (US) equipment and at investigating its relationship to lifestyle characteristics and dietary habits. Participants and methods: The current study was part of the China Hainan Centenarian Cohort Study that was conducted in Hainan, an iodine-sufficient region in People's Republic of China...
2018: Clinical Interventions in Aging
C Sethulekshmi, C Latha, C J Anu
Aim: The objective of the study was to detect Shiga toxin-producing Escherichia coli (STEC) and develop a quantitative polymerase chain reaction (qPCR) assay to quantify the bacterial DNA present in different food matrices. Materials and Methods: A total of 758 samples were collected during a period from January 2015 to December 2016 from Kozhikode, Thrissur, and Alappuzha districts of Kerala. The samples consisted of raw milk (135), pasteurized milk (100), beef (132), buffalo meat (130), chevon (104), beef kheema (115), and beef sausage (42)...
February 2018: Veterinary World
Chih-Yuan Ko, Xiao-Yu Chen, Wen-Chang Chang, Yi-Ming Zeng, Ru-Hai Lin, Xiao-Bin Zhang, James Swi-Bea Wu, Szu-Chuan Shen
BACKGROUND: Marinating meat with alcohol, such as wine and beer, is a common culinary practice in cultures worldwide. This study we use a model marination solution comprising 0.2 M glucose-0.2 M glycine buffered to pH 4.3 containing either 0% or 50% ethanol and mimicked the cooking process by heating for 12 h. Antioxidative and antimutagenic characteristics of Maillard reaction products (MRPs) were investigated. Reducing power, antioxidant activity (Fe2+ chelating ability) and free radical neutralization ability generated from DPPH and ABTS were determined...
April 12, 2018: Journal of the Science of Food and Agriculture
Seongjin Oh, David Tinotenda Mbiriri, Chaehwa Ryu, Kangheon Lee, Sangbuem Cho, Nag-Jin Choi
Objective: The goal of this study was to evaluate kenaf as a roughage source in vitro and its effects on meat quality of Hanwoo (Korean native) cattle. Methods: Three roughage materials, rice straw silage, ryegrass silage, and kenaf silage, were tested in a batch culture and feeding trial. Rumen fermentation parameters, including gas, pH, volatile fatty acid, and ammonia were analyzed. In the feeding trial, Hanwoo steers (373.5 ± 5.1 kg, n = 36, 11 month of age) were divided into three feeding groups (n = 12 each)...
April 12, 2018: Asian-Australasian Journal of Animal Sciences
A B Alzwghaibi, R Yahyaraeyat, B Nayeri Fasaei, A Ghalyanchi Langeroudi, T Zahraei Salehi
Salmonella is widely distributed throughout the world and can be found in poultry industry, animal breeding centers, food and feedstuffs of all geographical regions. This study was conducted to determine and identify Salmonella serovars isolated from poultry, calves and foodstuffs (poultry and animals products such as egg and meat). A total of one hundred isolates of Salmonella serovars including Salmonella Typhimurium, Salmonella Enteritidis, Salmonella Infantis, Salmonella Gallinarum and Salmonella Pullorum consecutively were subjected to the conventional culture, biochemical and serological assays...
April 7, 2018: Archives of Microbiology
Daniel Eibach, Denise Dekker, Kennedy Gyau Boahen, Charity Wiafe Akenten, Nimako Sarpong, Cristina Belmar Campos, Laura Berneking, Martin Aepfelbacher, Ralf Krumkamp, Ellis Owusu-Dabo, Jürgen May
Antibiotic use in animal husbandry has raised concerns on the spread of resistant bacteria. Currently animal products are traded globally with unprecedented ease, which has been challenging the control of antimicrobial resistance. This study aims to detect and characterize extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from imported and locally produced poultry products sold in Ghana. Local and imported chicken meat was collected from 94 stores and markets throughout Kumasi (Ghana) and cultured on selective ESBL screening agar...
April 2018: Veterinary Microbiology
Janette M Shank, Brittni R Kelley, Joseph W Jackson, Jessica L Tweedie, Dana Franklin, Steven M Damo, Jennifer A Gaddy, Caitlin N Murphy, Jeremiah G Johnson
Campylobacter jejuni is a leading cause of bacterial-derived gastroenteritis worldwide. Campylobacter is most commonly acquired through the consumption of undercooked poultry meat or through drinking contaminated water. Following ingestion, Campylobacter adheres to the intestinal epithelium and mucus layer, causing toxin-mediated inflammation and inhibition of fluid reabsorption. Currently, the human response to infection is relatively unknown and animal hosts that model those responses are rare. As such, we examined patient fecal samples for the accumulation of the neutrophil protein, calgranulin C, during infection with Campylobacter jejuni...
April 2, 2018: Infection and Immunity
Angelina Münster, Corina Knipper, Vicky M Oelze, Nicole Nicklisch, Marcus Stecher, Björn Schlenker, Robert Ganslmeier, Matthias Fragata, Susanne Friederich, Veit Dresely, Vera Hubensack, Guido Brandt, Hans-Jürgen Döhle, Werner Vach, Ralf Schwarz, Carola Metzner-Nebelsick, Harald Meller, Kurt W Alt
Investigation of human diet during the Neolithic has often been limited to a few archaeological cultures or single sites. In order to provide insight into the development of human food consumption and husbandry strategies, our study explores bone collagen carbon and nitrogen isotope data from 466 human and 105 faunal individuals from 26 sites in central Germany. It is the most extensive data set to date from an enclosed geographic microregion, covering 4,000 years of agricultural history from the Early Neolithic to the Early Bronze Age...
2018: PloS One
Alejandra Orihuel, Julieta Bonacina, María José Vildoza, Elena Bru, Graciela Vignolo, Lucila Saavedra, Silvina Fadda
The aim of this work was to evaluate the effect of meat curing agents on the bioprotective activity of the bacteriocinogenic strain, Enterococcus (E.) mundtii CRL35 against Listeria (L.) monocytogenes during meat fermentation. The ability of E. mundtii CRL35 to grow, acidify and produce bacteriocin in situ was assayed in a meat model system in the presence of curing additives (CA). E. mundtii CRL35 showed optimal growth and acidification rates in the presence of CA. More importantly, the highest bacteriocin titer was achieved in the presence of these food agents...
May 2018: Food Research International
Orsolya Erdősi, Katalin Szakmár, Zsuzsanna Szili, Géza Szita, Sándor Bernáth, József Sövényi, Péter Laczay
The rapid detection of Campylobacter spp. is of utmost importance for the reduction of infections in humans by contaminated food products. The standard culturing method (ISO 10272-1:2006) involves a high time and labour demand. In this paper, we present a method that reduces the detection time of Campylobacter spp. to or below one third as compared to the ISO method, at a reduced cost per test. We used redox potential change of enrichment cultures (Bolton broth with Bolton selective supplement) for reliably selecting Campylobacter-contaminated raw milk and broiler meat samples...
March 2018: Acta Veterinaria Hungarica
Mostafa Y Abdel-Glil, Prasad Thomas, Gernot Schmoock, Kamel Abou-El-Azm, Lothar H Wieler, Heinrich Neubauer, Christian Seyboldt
C. difficile has been recognized as a potential zoonotic agent encouraging investigations of C. difficile prevalence and ribotypes in animals. Here we report the prevalence and diversity of Egyptian C. difficile in I) samples from healthy poultry (n = 50), II) samples from diseased poultry (n = 54), and III) poultry meat (n = 150). Thirteen isolates were obtained from seven healthy and five diseased animals, but no C. difficile was cultured from poultry meat. The isolated C. difficile strains belonged to 3 different PCR-ribotypes (039/2, 205 and 001/FLI01)...
March 20, 2018: Anaerobe
Natalia Vasilyevna Rudenko, Anna Petrovna Karatovskaya, Anatolyi Nikolaevich Noskov, Anna Olegovna Shepelyakovskaya, Margarita Pavlovna Shchannikova, Irina Vladimirovna Loskutova, Olga Anatolievna Artyemieva, Daria Alexandrovna Nikanova, Elena Alexandrovna Gladyr, Fedor Alexandrovich Brovko
Staphylococcal enterotoxins cause food poisoning of various degrees of severity. For milk and meat products, there is a high probability of contamination with staphylococcal enterotoxin H (SEH). In this regard specific and sensitive methods are required to be developed for its detection and monitoring. In this work, the gene seh was expressed and a preparation of recombinant toxin was obtained. Using hybridoma technology, a panel of high-affinity monoclonal antibodies (mAbs) to SEH was produced. The antibodies were characterized and shown to have no cross-reactivity towards the main staphylococcal enterotoxins (A, B, C1, D, E, G and I)...
April 2018: Journal of Food and Drug Analysis
Germana Giuggioli, Alberto Olivastri, Luca Pennisi, Domenico Paludi, Adriana Ianieri, Alberto Vergara
The use of game meat as a food source is currently a growing trend in our country. These products have strong and historic ties with cultural and culinary tradition, but are also appreciated for their sensory and nutritional characteristics. A major contributor to the supply of this type of product is hunting. Practiced since the dawn of time for survival, hunting has evolved into a recreational activity with substantial commercial interests. Of particular importance in this context is hunting of large ungulates...
October 20, 2017: Italian Journal of Food Safety
Christoph Jans, Eleonora Sarno, Lucie Collineau, Leo Meile, Katharina D C Stärk, Roger Stephan
Background: Antimicrobial resistance (AMR) in bacteria is an increasing health concern. The spread of AMR bacteria (AMRB) between animals and humans via the food chain and the exchange of AMR genes requires holistic approaches for risk mitigation. The AMRB exposure of humans via food is currently only poorly understood leaving an important gap for intervention design. Method: This study aimed to assess AMRB prevalence in retail food and subsequent exposure of Swiss consumers in a systematic literature review of data published between 1996 and 2016 covering the Swiss agriculture sector and relevant imported food...
2018: Frontiers in Microbiology
Xiaoping Su, Kuiqing Cui, Shanshan Du, Hongli Li, Fenghua Lu, Deshun Shi, Qingyou Liu
Myostatin (MSTN), a protein encoded by growth differentiation factor 8 (GDF8), is primarily expressed in skeletal muscle and negatively regulates the development and regeneration of muscle. Accordingly, myostatin-deficient animals exhibit a double-muscling phenotype. The CRISPR/Cas9 system has proven to be an efficient genome-editing tool and has been applied to gene modification in cells from many model organisms such as Drosophila melanogaster, zebrafish, mouse, rat, sheep, and human. Here, we edited the GDF8 gene in fibroblasts and embryos of Debao pig and swamp buffalo using the CRISPR/Cas9 system...
March 19, 2018: In Vitro Cellular & Developmental Biology. Animal
Ayaka Ishihara, Etaro Hashimoto, Haruhiko Ishioka, Hiroyuki Kobayashi, Harumi Gomi
Meningitis caused by the zoonotic pathogen Campylobacter fetus in immunocompetent adults is rare. We report a 48-year-old Japanese woman with no underlying disease who was found to have meningitis caused by C. fetus . Both C. fetus subsp. fetus and C. fetus subsp. venerealis were isolated from the cerebrospinal fluid culture. The mode of infection in our patient was considered to be associated with the consumption of raw beef and raw cattle liver on a regular basis. Public awareness and education to avoid the consumption of raw or undercooked meat might help prevent C...
2018: IDCases
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