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https://www.readbyqxmd.com/read/29034895/generation-of-induced-pluripotent-stem-cell-ipsc-line-from-charcot-marie-tooth-disease-patient-with-mpz-mutation-cmt1b
#1
Daryeon Son, Phil Jun Kang, Wonjin Yun, Seungkwon You
Charcot-Marie-Tooth disease (CMT1B) is an inherited neurological disorder caused by mutation of the myelin protein zero (MPZ) gene. We generated an induced pluripotent stem cell (iPSC) line from an 81-year-old patient with CMT1B by electroporating of lymphoblastoid cell lines with episomal plasmids encoding OCT4, SOX2, KLF4, L-MYC, LIN28, and p53-targeting shRNA. The established iPSCs expressed various pluripotency markers, demonstrated the potential to differentiate into cells of the three germ layers in vitro, had a normal karyotype and retained the MPZ mutation...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29034893/establishment-of-dyt5-patient-specific-induced-pluripotent-stem-cells-with-a-gch1-mutation
#2
Nagahisa Murakami, Taizo Ishikawa, Takayuki Kondo, Keiko Imamura, Kayoko Tsukita, Takako Enami, Misato Funayama, Ran Shibukawa, Shinichi Matsumoto, Yuishin Izumi, Etsuro Ohta, Fumiya Obata, Ryuji Kaji, Haruhisa Inoue
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 20-year-old dystonia patient with a GCH1 mutation (DYT5). Episomal vectors were used to introduce reprogramming factors (OCT3/4, SOX2, KLF4, L-MYC, LIN28, and p53 carboxy-terminal dominant-negative fragment) to the PBMCs. The generated iPSCs expressed pluripotency markers, and were capable of differentiating into derivates of all three germ layers in vitro. The iPSC line also showed a normal karyotype and preserved the GCH1 mutation...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29034889/generation-of-a-pig-induced-pluripotent-stem-cell-pipsc-line-from-embryonic-fibroblasts-by-incorporating-lin28-to-the-four-transcriptional-factor-mediated-reprogramming-vsmui001-d
#3
Warunya Chakritbudsabong, Ladawan Sariya, Sirikron Pamonsupornvichit, Rassmeepen Pronarkngver, Somjit Chaiwattanarungruengpaisan, Joao N Ferreira, Piyathip Setthawong, Praopilas Phakdeedindan, Mongkol Techakumphu, Theerawat Tharasanit, Sasitorn Rungarunlert
Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28955403/expression-of-ercc1-bcl-2-lin28a-and-ki-67-as-biomarkers-of-response-to-first-line-platinum-based-chemotherapy-in-patients-with-high-grade-extrapulmonary-neuroendocrine-carcinomas-or-small-cell-lung-cancer
#4
Juliana Florinda de M Rêgo, Raphael Salles Scortegagna de Medeiros, Maria Ignez Braghiroli, Breno Galvão, João Evangelista Bezerra Neto, Rodrigo Ramella Munhoz, Juliana Guerra, Suely Nonogaki, Lidia Kimura, Tulio Eduardo Pfiffer, Gilberto de Castro, Paulo Marcelo Hoff, Duilio Rocha Filho, Frederico Perego Costa, Rachel P Riechelmann
BACKGROUND: Small cell lung cancer (SCLC) and high-grade extrapulmonary neuroendocrine carcinomas (EPNEC) share similar histopathological features and treatment, but outcomes may differ. We evaluated in our study the expression of biomarkers associated with response rate (RR) to chemotherapy and overall survival (OS) for these entities. MATERIALS AND METHODS: This is a multicentre retrospective analysis of advanced EPNEC and SCLC patients treated with platinum-based chemotherapy...
2017: Ecancermedicalscience
https://www.readbyqxmd.com/read/28952926/generation-of-a-human-induced-pluripotent-stem-cell-line-cerai001-a-6-using-episomal-vectors
#5
Raymond C B Wong, Sandy S Hung, Stacey Jackson, Vikrant Singh, Shahnaz Khan, Helena H Liang, Lisa S Kearns, Tu Nguyen, Alison Conquest, Maciej Daniszewski, Alex W Hewitt, Alice Pébay
We report the generation of the hiPSC line CERAi001-A-6 from primary human dermal fibroblasts. Reprogramming was performed using episomal vector delivery of OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA for p53.
July 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28947981/lin28b-facilitates-the-progression-and-metastasis-of-pancreatic-ductal-adenocarcinoma
#6
Yunchao Wang, Jian Li, Shixiang Guo, Yongsheng Ouyang, Liangyu Yin, Songsong Liu, Zhiping Zhao, Jiali Yang, Wenjie Huang, Huan Qin, Xin Zhao, Bing Ni, Huaizhi Wang
Lin28B, a Lin28 homologue, represses the biogenesis of let-7 microRNAs (miRNAs), has a role in tumorigenesis, and is considered a potential therapeutic target for various human malignancies. However, the associations between Lin28B and the clinical features and outcomes of patients with pancreatic ductal adenocarcinoma (PDAC) remain unclear. In this study, we explored the clinical significance of Lin28B in PDAC and its association with metastasis by examining tissues from patients with PDAC and elucidated the molecular mechanisms using PDAC cell lines...
September 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28947139/lncrna-hottip-modulates-cancer-stem-cell-properties-in-human-pancreatic-cancer-by-regulating-hoxa9
#7
Zhiqiang Fu, Changhao Chen, Quanbo Zhou, Yinxue Wang, Yue Zhao, Xiaohui Zhao, Wenzhu Li, Shangyou Zheng, Huilin Ye, Lin Wang, Zhanghai He, Qing Lin, Zhihua Li, Rufu Chen
Our previous study demonstrated that long non-coding RNA (lncRNA) HOTTIP was maximally expressed in PDAC, and promoted cancer cell progression and epithelial to mesenchymal transition (EMT). Numerous studies indicated that lncRNAs or EMT supported cancer stem cells. However, the role of HOTTIP in pancreatic cancer stem cells (PCSCs) remains unclear. Here, we evaluated the role and mechanism of HOTTIP in PCSCs. First, we analyzed the relationship between HOTTIP expression and overall or disease-free survival in 90 patients with PDAC after radical resection...
September 22, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28945502/comparative-analysis-of-lin28-rna-binding-sites-identified-at-single-nucleotide-resolution
#8
Elizabeth Ransey, Anders Björkbom, Victor S Lelyveld, Przemyslaw Biecek, Lorena Pantano, Jack W Szostak, Piotr Sliz
It remains a formidable challenge to characterize the diverse complexes of RNA binding proteins and their targets. While crosslink and immunoprecipitation (CLIP) methods are powerful techniques that identify RNA targets on a global scale, the resolution and consistency of these methods is a matter of debate. Here we present a comparative analysis of LIN28-pre-let-7 UV-induced crosslinking using a tandem mass spectrometry (MS/MS) and deep sequencing interrogation of in vitro crosslinked complexes. Interestingly, analyses by the two methods diverge in their identification of crosslinked nucleotide identity - whereas bioinformatics and sequencing analyses suggest guanine in mammalian cells, MS/MS identifies uridine...
September 25, 2017: RNA Biology
https://www.readbyqxmd.com/read/28910332/concurrent-treatment-with-simvastatin-and-nf-%C3%AE%C2%BAb-inhibitor-in-human-castration-resistant-prostate-cancer-cells-exerts-synergistic-anti-cancer-effects-via-control-of-the-nf-%C3%AE%C2%BAb-lin28-let-7-mirna-signaling-pathway
#9
Minyong Kang, Kyoung-Hwa Lee, Hye Sun Lee, Chang Wook Jeong, Ja Hyeon Ku, Hyeon Hoe Kim, Cheol Kwak
We examined the anti-cancer effects and molecular mechanism of simvastatin in human castration-resistant prostate cancer (CRPC) cells, particularly focused on LIN28B and its target molecule, let-7 microRNA (miRNA) among the various target genes of NF-κB. A human CRPC cell line (PC3) was used in the current study. Gene expression patterns were evaluated using real time-PCR and western blot analysis. CCK-8 assay was used for assessing cell viability and proliferation, and a clonogenic assay was adopted to evaluate clonal proliferative capabilities...
2017: PloS One
https://www.readbyqxmd.com/read/28898112/functional-odontoblastic-like-cells-derived-from-human-ipscs
#10
H Xie, N Dubey, W Shim, C J A Ramachandra, K S Min, T Cao, V Rosa
The induced pluripotent stem cells (iPSCs) have an intrinsic capability for indefinite self-renewal and large-scale expansion and can differentiate into all types of cells. Here, we tested the potential of iPSCs from dental pulp stem cells (DPSCs) to differentiate into functional odontoblasts. DPSCs were reprogrammed into iPSCs via electroporation of reprogramming factors OCT-4, SOX2, KLF4, LIN28, and L-MYC. The iPSCs presented overexpression of the reprogramming genes and high protein expressions of alkaline phosphatase, OCT4, and TRA-1-60 in vitro and generated tissues from 3 germ layers in vivo...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28892074/replacing-reprogramming-factors-with-antibodies-selected-from-combinatorial-antibody-libraries
#11
Joel W Blanchard, Jia Xie, Nadja El-Mecharrafie, Simon Gross, Sohyon Lee, Richard A Lerner, Kristin K Baldwin
The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) is usually achieved by exogenous induction of transcription by factors acting in the nucleus. In contrast, during development, signaling pathways initiated at the membrane induce differentiation. The central idea of this study is to identify antibodies that can catalyze cellular de-differentiation and nuclear reprogramming by acting at the cell surface. We screen a lentiviral library encoding ∼100 million secreted and membrane-bound single-chain antibodies and identify antibodies that can replace either Sox2 and Myc (c-Myc) or Oct4 during reprogramming of mouse embryonic fibroblasts into iPSCs...
October 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28886879/analysis-of-putative-biomarkers-of-undifferentiated-spermatogonia-in-dog-testis
#12
Won-Young Lee, Hyun-Jung Park, Ran Lee, Ji-Heon Lee, Hyunjhung Jhun, Tai-Young Hur, Hyuk Song
Spermatogenesis begins after puberty and continues throughout a male's life, and is regulated by spermatogonial stem cells in the seminiferous tubules. Markers of male germ cells, including undifferentiated spermatogonia to fully developed spermatozoa have been identified in rodents, but not in dogs. In this study, to characterize the markers of undifferentiated spermatogonia, histological and immunohistochemical analyses were performed on pre-pubertal (1-month-old), early pubertal (4-month-old), and post-pubertal (7-month-old) dog testes...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28884008/efficient-method-to-create-integration-free-virus-free-myc-and-lin28-free-human-induced-pluripotent-stem-cells-from-adherent-cells
#13
Anant Kamath, Sara Ternes, Stephen McGowan, Anthony English, Rama Mallampalli, Alan B Moy
AIM: Nonviral induced pluripotent stem cell (IPSC) reprogramming is not efficient without the oncogenes, Myc and Lin28. We describe a robust Myc and Lin28-free IPSC reprogramming approach using reprogramming molecules. METHODS: IPSC colony formation was compared in the presence and absence of Myc and Lin28 by the mixture of reprogramming molecules and episomal vectors. RESULTS: While more colonies were observed in cultures transfected with the aforementioned oncogenes, the Myc and Lin28-free method achieved the same reprogramming efficiency as reports that used these oncogenes...
August 2017: Future Science OA
https://www.readbyqxmd.com/read/28846452/concise-review-lin28-let-7-signaling-a-critical-double-negative-feedback-loop-during-pluripotency-reprogramming-and-tumorigenicity
#14
Maryam Farzaneh, Farnoosh Attari, Seyed Esmaeil Khoshnam
MicroRNAs (miRNAs) with 20-30 nucleotides have recently emerged as the multidimensional regulators of cell fate decisions. Recent improvement in high-throughput sequencing has highlighted the potential role of LIN28/let-7 regulatory network in several developmental events. It was proposed that this pathway might represent a functional signature in cell proliferation, transition between commitment and pluripotency, and regulation of cancer and tumorigenicity. LIN28/let-7 regulatory pathway is one of the excellent examples of the relationship between an miRNA and mRNAs...
October 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28820723/a-lncrna-fine-tunes-the-dynamics-of-a-cell-state-transition-involving-lin28-let-7-and-de-novo-dna-methylation
#15
Meng Amy Li, Paulo P Amaral, Priscilla Cheung, Jan H Bergmann, Masaki Kinoshita, Tüzer Kalkan, Meryem Ralser, Sam Robson, Ferdinand von Meyenn, Maike Paramor, Fengtang Yang, Caifu Chen, Jennifer Nichols, David L Spector, Tony Kouzarides, Lin He, Austin Smith
Execution of pluripotency requires progression from the naïve status represented by mouse embryonic stem cells (ESCs) to a state capacitated for lineage specification. This transition is coordinated at multiple levels. Non-coding RNAs may contribute to this regulatory orchestra. We identified a rodent-specific long non-coding RNA (lncRNA) linc1281, hereafter Ephemeron (Eprn), that modulates the dynamics of exit from naïve pluripotency. Eprn deletion delays the extinction of ESC identity, an effect associated with perduring Nanog expression...
August 18, 2017: ELife
https://www.readbyqxmd.com/read/28768505/tough-decoy-targeting-of-predominant-let-7-mirna-species-in-adult-human-hematopoietic-cells
#16
Jaira F de Vasconcellos, Colleen Byrnes, Y Terry Lee, Joshua M Allwardt, Megha Kaushal, Antoinette Rabel, Jeffery L Miller
BACKGROUND: In humans, the heterochronic cascade composed of the RNA-binding protein LIN28 and its major target, the let-7 family of microRNAs (miRNAs), is highly regulated during human erythroid ontogeny. Additionally, down-regulation of the let-7 miRNAs in cultured adult CD34(+) cells or the over-expression of LIN28 in cultured erythrocytes from pediatric patients with HbSS genotype causes increased levels of fetal hemoglobin (HbF) in the range of 19-40% of the total. Therefore, we hypothesized that focused targeting of individual let-7 miRNA family members would exhibit regulatory effect on HbF expression in human adult erythroblasts...
August 2, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28745393/beyond-an-oncogene-lin28-is-a-master-regulator-of-cancer-progression
#17
REVIEW
Xuefei Wang, Mingjiao Weng, Yinji Jin, Weiwei Yang, Xin Wang, Di Wu, Tianzhen Wang, Xiaobo Li
The RNA binding protein Lin28 is increased in most human malignancies, and elevated Lin28 is a biomarker for poor prognosis and contributes to cancer progression. Lin28 functions as a master oncogene and is involved in almost all hallmarks of cancer. In this review, we summarize the aberrant molecular expression mechanisms and pathological roles of Lin28 in cancer progression. Moreover, we elaborate on the established molecular mechanisms, from the transcriptional level to the post-transcriptional and translational levels, by which Lin28 regulates cancer progression...
July 26, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28737214/decline-in-cellular-function-of-aged-mouse-c-kit-cardiac-progenitor-cells
#18
Alessandra Castaldi, Ramsinh Mansinh Dodia, Amabel M Orogo, Cristina M Zambrano, Rita H Najor, Åsa B Gustafsson, Joan Heller Brown, Nicole H Purcell
KEY POINTS: While autologous stem cell-based therapies are currently being tested on elderly patients, there are limited data on the function of aged stem cells and in particular c-kit(+) cardiac progenitor cells (CPCs). We isolated c-kit(+) cells from young (3 months) and aged (24 months) C57BL/6 mice to compare their biological properties. Aged CPCs have increased senescence, decreased stemness and reduced capacity to proliferate or to differentiate following dexamethasone (Dex) treatment in vitro, as evidenced by lack of cardiac lineage gene upregulation...
October 1, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28732738/association-of-microrna-125a-and-microrna-499a-polymorphisms-in-chronic-periodontitis-in-a-sample-south-indian-population-a-hospital-based-genetic-association-study
#19
Priyanka Venugopal, Vamsi Lavu, Suresh Ranga Rao, Vettriselvi Venkatesan
Periodontitis is a chronic inflammatory disease, caused by interaction between periodontopathic bacteria and the host immune response. MicroRNAs are small, single-stranded molecules, which play a key role in the regulation of diverse biological processes. Dysregulation of microRNAs function can lead to several diseases such as autoimmune and chronic inflammatory diseases. The objective of the study was to determine the association between selected single nucleotide polymorphisms in miR-125a, miR-499 and LIN28 homology A with chronic periodontitis susceptibility in a sample population from south India...
October 5, 2017: Gene
https://www.readbyqxmd.com/read/28703793/linc-dync2h1-4-promotes-emt-and-csc-phenotypes-by-acting-as-a-sponge-of-mir-145-in-pancreatic-cancer-cells
#20
Yuran Gao, Zhicheng Zhang, Kai Li, Liying Gong, Qingzhu Yang, Xuemei Huang, Chengcheng Hong, Mingfeng Ding, Huanjie Yang
The acquisition of epithelial-mesenchymal transition (EMT) and/or existence of a sub-population of cancer stem-like cells (CSC) are associated with malignant behavior and chemoresistance. To identify which factor could promote EMT and CSC formation and uncover the mechanistic role of such factor is important for novel and targeted therapies. In the present study, we found that the long intergenic non-coding RNA linc-DYNC2H1-4 was upregulated in pancreatic cancer cell line BxPC-3-Gem with acquired gemcitabine resistance...
July 13, 2017: Cell Death & Disease
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