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https://www.readbyqxmd.com/read/29603519/comparison-of-two-different-culture-conditions-for-derivation-of-early-hipsc
#1
Caroline Amalie Brunbjerg Hey, Katarina Beata Saltoõkova, Hanne Cathrine Bisgaard, Lisbeth Birk Møller
Different culture-systems for derivation of induced pluripotent stem cells (iPSC) in vitro from human fibroblasts have been established. Here we compared the efficacy of two different feeder-free culture-systems; Matrigel-coated surfaces in combination with mTeSR1 medium versus Vitronectin-coated surfaces in combination with Essential 8 (E8) medium. The comparison was performed by counting the number of emerging iPSC-looking colonies of re-programmed fibroblasts. The fibroblasts were re-programmed using episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 knock down shP53...
March 30, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29602048/lymphoblast-derived-integration-free-ipsc-line-ad-trem2-1-from-a-67year-old-alzheimer-s-disease-patient-expressing-the-trem2-p-r47h-variant
#2
Soraia Martins, Hatice Yigit, Martina Bohndorf, Nina Graffmann, Aurelian Robert Fiszl, Wasco Wruck, Kristel Sleegers, Christine Van Broeckhoven, James Adjaye
Human lymphoblast cells from a male diagnosed with Alzheimer's disease (AD) expressing the TREM2 p.R47H variant were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids harbouring OCT4, SOX2, NANOG, LIN28, c-MYC and L-MYC. AD-TREM2-1 was defined as pluripotent based on (i) expression of pluripotency-associated markers (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptome of the iPSC line and the human embryonic stem cell line H1 with a Pearson correlation of 0...
March 20, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29552298/synergistic-neuroprotective-effect-of-rasagiline-and-idebenone-against-retinal-ischemia-reperfusion-injury-via-the-lin28-let-7-dicer-pathway
#3
Dawei Lei, Zhengbo Shao, Xinrong Zhou, Huiping Yuan
Retinal ischemia-reperfusion (RIR) injury causes neuronal degeneration and initiates various optic nerve diseases. This study aimed to investigate the synergistic neuroprotective effect of rasagiline and idebenone against RIR injury. A combination of rasagiline and idebenone was administered intraperitoneally immediately after establishment of the RIR model. Treatment with the combination of the two drugs resulted in a significant restoration of retinal thickness and retinal ganglion cells. Apoptosis of cells in ganglion cell layers was also ameliorated, suggesting that the effect of the two drugs was synergistic and the expression of brain-derived neurotrophic factor increased...
February 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29540527/a-biosensor-for-mapk-dependent-lin28-signaling
#4
Laurel M Oldach, Kirill Gorshkov, William T Mills, Jin Zhang, Mollie K Meffert
Intracellular levels of the RNA-binding protein and pluripotency factor, Lin28a, are tightly controlled to govern cellular and organismal growth. Lin28a is extensively regulated at the post-transcriptional level, and can undergo mitogen-activated protein kinase (MAPK)-mediated elevation from low basal levels in differentiated cells by phosphorylation-dependent stabilizing interaction with the RNA-silencing factor HIV TAR-RNA-binding protein (TRBP). However, molecular and spatio-temporal details of this critical control mechanism remained unknown...
March 14, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29510730/the-lncrna-macc1-as1-promotes-gastric-cancer-cell-metabolic-plasticity-via-ampk-lin28-mediated-mrna-stability-of-macc1
#5
Yang Zhao, Yajing Liu, Li Lin, Qiong Huang, Wanming He, Shuyi Zhang, Shumin Dong, Zhaowei Wen, Jinjun Rao, Wangjun Liao, Min Shi
BACKGROUND: Metabolic plasticity has been increasingly thought to be a determinant of tumor growth and metastasis. MACC1, a transcriptional regulator of MET, was recognized as an oncogene in gastric cancer (GC); however, its transcriptional or post-translational regulation was not clear. We previously reported the metabolic role of MACC1 in glycolysis to promote GC progression. MACC1-AS1 is the antisense lncRNA of MACC1, yet its function was previously unknown. METHODS: We profiled and analyzed the expression of MACC1-AS1 utilizing the TCGA database as well as in situ hybridization using 123 pairs of GC tissues and matched adjacent normal gastric mucosa tissues (ANTs)...
March 6, 2018: Molecular Cancer
https://www.readbyqxmd.com/read/29499498/generation-of-a-human-ipsc-line-by-mrna-reprogramming
#6
Ana Artero Castro, Marian León, Verónica Del Buey Furió, Slaven Erceg, Dunja Lukovic
The human iPSC cell line, derived from foreskin fibroblasts was generated by non-integrative, non-viral reprogramming technology using OCT4, SOX2, KLF4, LIN28, c-MYC mRNAs.
April 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29487310/immunologic-targeting-of-cd30-eliminates-tumourigenic-human-pluripotent-stem-cells-allowing-safer-clinical-application-of-hipsc-based-cell-therapy
#7
Nagako Sougawa, Shigeru Miyagawa, Satsuki Fukushima, Ai Kawamura, Junya Yokoyama, Emiko Ito, Akima Harada, Kaori Okimoto, Noriko Mochizuki-Oda, Atsuhiro Saito, Yoshiki Sawa
Induced pluripotent stem cells (iPSCs) are promising candidate cells for cardiomyogenesis in the failing heart. However, teratoma/tumour formation originating from undifferentiated iPSCs contaminating the graft is a critical concern for clinical application. Here, we hypothesized that brentuximab vedotin, which targets CD30, induces apoptosis in tumourigenic cells, thus increasing the safety of iPSC therapy for heart failure. Flow cytometry analysis identified consistent expression of CD30 in undifferentiated human iPSCs...
February 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29462197/thymic-epithelial-cell-derived-signals-control-b-progenitor-formation-and-proliferation-in-the-thymus-by-regulating-let-7-and-arid3a
#8
Shiyun Xiao, Wen Zhang, Nancy R Manley
The postnatal thymus is an efficient microenvironment for T cell specification and differentiation. B cells are also present in the thymus and have been recently shown to impact T cell selection, however, the mechanisms controlling B cell development in the thymus are largely unknown. In Foxn1lacZ mutant mice, down-regulation of Foxn1 expression in thymic epithelial cells beginning 1 week after birth caused a dramatic reduction of T progenitors and an increase of B cell progenitors. This time point is coincident with the switch from fetal to adult-type hematopoietic stem cells (HSCs), which is regulated by the Lin28-Let7 system...
2018: PloS One
https://www.readbyqxmd.com/read/29423528/cellular-functions-of-stem-cell-factors-mediated-by-the-ubiquitin-proteasome-system
#9
REVIEW
Jihye Choi, Kwang-Hyun Baek
Stem cells undergo partitioning through mitosis and separate into specific cells of each of the three embryonic germ layers: endoderm, mesoderm, and ectoderm. Pluripotency, reprogramming, and self-renewal are essential elements of embryonic stem cells (ESCs), and it is becoming evident that regulation of protein degradation mediated by the ubiquitin-proteasome system (UPS) is one of the key cellular mechanisms in ESCs. Although the framework of that mechanism may seem simple, it involves complicated proteolytic machinery...
February 8, 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29416635/otud7b-upregulation-predicts-a-poor-response-to-paclitaxel-in-patients-with-triple-negative-breast-cancer
#10
Hui-Wen Chiu, Hui-Yu Lin, Ing-Jy Tseng, Yuan-Feng Lin
Paclitaxel is a first-line chemotherapeutic for patients with breast cancer, particularly triple-negative breast cancer (TNBC). Molecular markers for predicting pathologic responses to paclitaxel treatment is thus urgently needed since paclitaxel resistance is still a clinical issue in treating TNBCs. We investigated the transcriptional profiling of consensus genes in HCC38 (paclitaxel-sensitive) and MDA-MB436 (paclitaxel-resistant) TNBC cells post-treatment with paclitaxel. We found that OTUD7B was downregulated in HCC38 but upregulated in MDA-MB436 cells after paclitaxel treatment at cytotoxic concentrations...
January 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29393461/lin28-microrna-let-7a-promotes-metastasis-under-circumstances-of-hyperactive-wnt-signaling-in-esophageal-squamous-cell-carcinoma
#11
Rui Ling, Yuepeng Zhou, Ling Zhou, Dongfang Dai, Dan Wu, Lei Mi, Chaoming Mao, Deyu Chen
Dysregulation of micro (mi)RNA-let-7 has been associated with the development and prognosis of multiple cancer types. Lin28, a RNA-binding protein, plays a conserved role in regulating the maturation of let-7 family proteins. However, few studies have focused on the effects of Lin28/let-7 on Wnt-activated esophageal squamous cell carcinoma (ESCC). Analysis of the expression of let-7a, let-7b and let-7c in clinical tissues revealed that lower let-7a expression was correlated with higher tumor node metastasis staging and recurrence in patients with ESCC...
February 2, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29393430/silencing-lin28-promotes-apoptosis-in-colorectal-cancer-cells-by-upregulating-let%C3%A2-7c-targeting-of-antiapoptotic-bcl2l1
#12
Haogang Zhang, Yaguang Zong, Gongcai Qiu, Ruichun Jia, Xunzheng Xu, Fujing Wang, Dequan Wu
Colorectal cancer (CRC) remains a primary contributor to cancer‑associated mortality. The Lin28/let‑7 axis has previously been verified to participate in numerous pathophysiological processes involved in CRC. However, the potential roles and underlying mechanisms of this axis in apoptosis during CRC remain to be fully elucidated. The present study aimed to evaluate the role and reveal the molecular mechanisms of the Lin28/let‑7 axis in the apoptosis of CRC cells. An MTT assay was conducted to assess the cell viability of HCT116 and HT29 CRC cells, and caspase‑3 activity was analyzed to measure the apoptosis of CRC cells...
January 25, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29380422/microrna-31-is-required-for-astrocyte-specification
#13
Gordon P Meares, Rajani Rajbhandari, Magda Gerigk, Chih-Liang Tien, Chenbei Chang, Samuel C Fehling, Amber Rowse, Kayln C Mulhern, Sindhu Nair, G Kenneth Gray, Nicolas F Berbari, Markus Bredel, Etty N Benveniste, Susan E Nozell
Previously, we determined microRNA-31 (miR-31) is a noncoding tumor suppressive gene frequently deleted in glioblastoma (GBM); miR-31 suppresses tumor growth, in part, by limiting the activity of NF-κB. Herein, we expand our previous studies by characterizing the role of miR-31 during neural precursor cell (NPC) to astrocyte differentiation. We demonstrate that miR-31 expression and activity is suppressed in NPCs by stem cell factors such as Lin28, c-Myc, SOX2 and Oct4. However, during astrocytogenesis, miR-31 is induced by STAT3 and SMAD1/5/8, which mediate astrocyte differentiation...
January 30, 2018: Glia
https://www.readbyqxmd.com/read/29378863/notch-suppression-collaborates-with-ascl1-and-lin28-to-unleash-a-regenerative-response-in-fish-retina-but-not-in-mice
#14
Fairouz Elsaeidi, Peter Macpherson, Elizabeth A Mills, Jonathan Jui, John G Flannery, Daniel Goldman
Muller glial cells (MG) in the zebrafish retina respond to injury by acquiring retinal stem cell characteristics. Thousands of gene expression changes are associated with this event. Key among these changes are the induction of Ascl1a and Lin28a, two reprogramming factors whose expression is necessary for retina regeneration. Whether these factors are sufficient to drive MG proliferation and subsequent neuronal-fate specification remains unknown. To test this, we conditionally expressed Ascl1a and Lin28a in the uninjured retina of male and female fish...
January 29, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29372665/dna-methylation-of-the-oct4a-enhancers-in-embryonal-carcinoma-cells-after-etoposide-treatment-is-associated-with-alternative-splicing-and-altered-pluripotency-in-reversibly-senescent-cells
#15
Mikhail Baryshev, Inna Inashkina, Kristine Salmina, Anda Huna, Thomas R Jackson, Jekaterina Erenpreisa
The epigenetic mechanisms underlying chemoresistance in cancer cells resulting from drug-induced reversible senescence are poorly understood. Chemoresistant ESC-like embryonal carcinoma PA1 cells treated with etoposide (ETO) were previously found to undergo prolonged G2 arrest with transient p53-dependent upregulation of opposing fate regulators, p21CIP1 (senescence) and OCT4A (self-renewal). Here we report on the analysis of the DNA methylation state of the distal enhancer (DE) and proximal enhancer (PE) of the Oct4A gene during this dual response...
2018: Cell Cycle
https://www.readbyqxmd.com/read/29336272/let-7-mirna-precursors-co-express-with-lin28b-in-cervical-cells
#16
Aida Margarita Zamora-Contreras, Luis Marat Alvarez-Salas
BACKGROUND: The let-7 microRNAs (miRNAs) are frequently dysregulated in carcinogenic processes, including cervical cancer. LIN28 proteins regulate let-7 biogenesis by binding to conserved sequences within the pre-miRNA structure. Nevertheless, recent research has shown that some let-7 miRNAs may escape LIN28 regulation. OBJECTIVE: Correlate pre-let-7 miRNAs and LIN28B levels in cervical cell lines with different malignancy and HPV content. METHODS: Pre-let-7 levels were determined by RTqPCR...
2018: MicroRNA
https://www.readbyqxmd.com/read/29274886/establishment-of-human-ipsc-line-nccsi003-a-from-cd34-cells-of-peripheral-blood-collected-during-apheresis-of-healthy-donor-from-indian-ethnicity
#17
Sophia Fernandes, Shruti Tembe, Prajakta Shinde, Sameer Melinkeri, Vaijayanti Kale, Lalita Limaye
We present generation of iPSCs from CD34+ cells isolated from peripheral blood, collected during apheresis of a healthy female individual. We nucleofected the CD34+ cells by episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative mutation in p53). The resultant colonies showed cobble-stone appearance and stained positive for alkaline phosphatase. The colonies demonstrated presence of pluripotency markers by immunofluorescence, flow-cytometry and PCR. The plasmids were lost from cells subsequently during passages as assessed by PCR...
March 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29259864/an-up-date-on-epigenetic-and-molecular-markers-in-testicular-germ-cell-tumors
#18
Paolo Chieffi
Testicular germ cell tumor (TGCT) is the most common solid malignancy occurring in young men between 20 and 34 years of age, and its incidence has increased significantly over the last decades. Clinically several types of immunohistochemical markers are useful and sensitive. These new biomarkers are genes expressed in primordial germ cells/gonocytes and embryonic pluripotency-related cells but not in normal adult germ cells and they include OCT3/4, HMGA1 and 2, NANOG, SOX2, and LIN28. Gene expression in TGCT is regulated, at least in part, by DNA and histone modifications, and the epigenetic profile of these tumours is characterised by genome-wide demethylation...
November 2017: Intractable & Rare Diseases Research
https://www.readbyqxmd.com/read/29250763/transplantation-of-spermatogonial-stem-cells-suspension-into-rete-testis-of-azoospermia-mouse-model
#19
Arefeh Jafarian, Niknam Lakpour, Mohammad Reza Sadeghi, Sheida Salehkhou, Mohammad Mehdi Akhondi
PURPOSE: The loss of spermatogonia following chemo-or radiotherapy leading to temporary or permanent infertility of the patient is a well known and unwanted side effect of many oncological therapies. MATERIALS AND METHODS: In this study, germ cells were isolated from 4 days old mouse testis cells. Busulfan treatment was used to the eliminate proliferating cells in the testis of recipient mice. The donor cells suspended in DMEM, were introduced into the rete testis of recipient mice via microinjection method...
January 23, 2018: Urology Journal
https://www.readbyqxmd.com/read/29246570/integration-free-induced-pluripotent-stem-cells-derived-from-a-patient-with-autosomal-recessive-alport-syndrome-aras
#20
Bernd Kuebler, Begoña Aran, Laia Miquel-Serra, Yolanda Muñoz, Elisabet Ars, Gemma Bullich, Monica Furlano, Roser Torra, Merce Marti, Anna Veiga, Angel Raya
A skin biopsy was obtained from a 25-year-old female patient with autosomal recessive Alport syndrome (ARAS) with the homozygous COL4A3 mutation c.345delG, p.(P166Lfs*37). Dermal fibroblasts were derived and reprogrammed by nucleofection with episomal plasmids carrying OCT3/4, SOX2, KLF4 LIN28, L-MYC and p53shRNA. The generated induced Pluripotent Stem Cell (iPSC) clone AS FiPS1 Ep6F-2 was free of genomically integrated reprogramming genes, had the specific homozygous mutation, a stable karyotype, expressed pluripotency markers and generated embryoid bodies which were differentiated towards the three germ layers in vitro...
December 2017: Stem Cell Research
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