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Lin28

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https://www.readbyqxmd.com/read/29218439/characterization-and-expression-of-lin-28a-involved-in-lin28-let-7signal-pathway-during-early-development-of-p-olivaceus
#1
Yuanshuai Fu, Lina Gao, Zhiyi Shi, Feng You, Junling Zhang, Wenjuan Li
Heterochronic lin-28 is a conserved RNA-binding protein that plays a key role in the timing of developmental events in organisms. As a crucial heterochronic gene, the protein controls developmental events of the second of four larval stages in Caenorhabditi elegans. Heterochronic let-7 miRNAs are often present in various species and highly conserved in sequence and biological function and are required for various biological processes. Previous studies showed that ten let-7 miRNAs were identified in the Japanese flounder (Paralichthys olivaceus) and that they were primarily expressed during metamorphosis...
December 7, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29212039/comprehensive-mapping-of-pluripotent-stem-cell-metabolism-using-dynamic-genome-scale-network-modeling
#2
Sriram Chandrasekaran, Jin Zhang, Zhen Sun, Li Zhang, Christian A Ross, Yu-Chung Huang, John M Asara, Hu Li, George Q Daley, James J Collins
Metabolism is an emerging stem cell hallmark tied to cell fate, pluripotency, and self-renewal, yet systems-level understanding of stem cell metabolism has been limited by the lack of genome-scale network models. Here, we develop a systems approach to integrate time-course metabolomics data with a computational model of metabolism to analyze the metabolic state of naive and primed murine pluripotent stem cells. Using this approach, we find that one-carbon metabolism involving phosphoglycerate dehydrogenase, folate synthesis, and nucleotide synthesis is a key pathway that differs between the two states, resulting in differential sensitivity to anti-folates...
December 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/29201231/genetic-variation-of-rs3811463-is-associated-with-gestational-diabetes-mellitus-susceptibility
#3
Yun Liu, Zhi-Ping Ge, Li-Zhou Sun, Pei Tong, Hong-Mei Lu
Gestational diabetes mellitus (GDM) is a growing health concern, and it increases the risk of adverse pregnancy outcomes with substantial long-term adverse health impacts on mothers and their offspring. Several studies have revealed specific associations between genetic variants and the risk of GDM. Single nucleotide polymorphisms (SNPs) are the major type of genetic variation in humans. Let-7 microRNA targets are enriched for genes containing SNPs associated with glucose metabolism, including Lin28. In the present study, the effect of T/C variants of rs3811463 (a SNP located near to the let-7 binding site in Lin28) on GDM risk was investigated...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29163026/generation-of-urine-cell-derived-non-integrative-human-ipscs-and-inscs-a-step-by-step-optimized-protocol
#4
Lin Cheng, Qiannan Lei, Chen Yin, Hui-Yun Wang, Kangxin Jin, Mengqing Xiang
Objective: Establishing a practical procedure to generate induced pluripotent stem cells (iPSCs) and induced neural stem cells (iNSCs) from human urine cells (UCs). In this report, we optimized a non-integrative protocol to generate patient-specific iPSC and iNSC lines with high reprogramming efficiency. Methods: UCs were electroporated with the pEP4-EO2S-ET2K and pEP4-M2L plasmids containing the OCT4, SOX2, KLF4, SV40LT, c-MYC, and LIN28 genes, and then cultured with N2B27 medium plus four small molecule compounds (A83-01, PD0325901, Thiazovivin, and CHIR99021)...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29162857/temporal-expression-pattern-of-genes-during-the-period-of-sex-differentiation-in-human-embryonic-gonads
#5
Linn S Mamsen, Emil H Ernst, Rehannah Borup, Agnete Larsen, Rasmus H Olesen, Erik Ernst, Richard A Anderson, Stine G Kristensen, Claus Y Andersen
The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150092/generation-of-integration-free-induced-pluripotent-stem-cell-lines-derived-from-two-patients-with-x-linked-alport-syndrome-xlas
#6
Bernd Kuebler, Begoña Aran, Laia Miquel-Serra, Yolanda Muñoz, Elisabet Ars, Gemma Bullich, Monica Furlano, Roser Torra, Merce Marti, Anna Veiga, Angel Raya
Skin biopsies were obtained from two male patients with X-linked Alport syndrome (XLAS) with hemizygous COL4A5 mutations in exon 41 or exon 46. Dermal fibroblasts were extracted and reprogrammed by nucleofection with episomal plasmids carrying OCT3/4, SOX2, KLF4 LIN28, L-MYC and p53 shRNA. The generated induced Pluripotent Stem Cell (iPSC) lines AS-FiPS2-Ep6F-28 and AS-FiPS3-Ep6F-9 were free of genomically integrated reprogramming genes, had the specific mutations, a stable karyotype, expressed pluripotency markers and generated embryoid bodies which were differentiated towards the three germ layers in vitro...
September 9, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29092089/notch-wnt-cross-signalling-regulates-stemness-of-dental-pulp-stem-cells-through-expression-of-neural-crest-and-core-pluripotency-factors
#7
V Uribe-Etxebarria, J Luzuriaga, P García-Gallastegui, A Agliano, F Unda, G Ibarretxe
Dental pulp stem cells (DPSCs) from adult teeth express neural crest (NC) markers together with core transcriptional factors associated with stem cell pluripotency, such as Oct4a, Sox2, c-Myc, Rex1, Stella/Dppa3, Ssea1/Fut4, Lin28 and Nanog. The possibility to boost the natural stemness features of DPSCs by mild methods, that do not involve gene and/or chromatin modification or gene transfection, is highly desirable for cell therapy. Canonical Wnt and Notch are two highly conserved developmental signalling pathways that are involved in NC emergence and stem cell self-renewal...
November 1, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/29034895/generation-of-induced-pluripotent-stem-cell-ipsc-line-from-charcot-marie-tooth-disease-patient-with-mpz-mutation-cmt1b
#8
Daryeon Son, Phil Jun Kang, Wonjin Yun, Seungkwon You
Charcot-Marie-Tooth disease (CMT1B) is an inherited neurological disorder caused by mutation of the myelin protein zero (MPZ) gene. We generated an induced pluripotent stem cell (iPSC) line from an 81-year-old patient with CMT1B by electroporating of lymphoblastoid cell lines with episomal plasmids encoding OCT4, SOX2, KLF4, L-MYC, LIN28, and p53-targeting shRNA. The established iPSCs expressed various pluripotency markers, demonstrated the potential to differentiate into cells of the three germ layers in vitro, had a normal karyotype and retained the MPZ mutation...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29034893/establishment-of-dyt5-patient-specific-induced-pluripotent-stem-cells-with-a-gch1-mutation
#9
Nagahisa Murakami, Taizo Ishikawa, Takayuki Kondo, Keiko Imamura, Kayoko Tsukita, Takako Enami, Misato Funayama, Ran Shibukawa, Shinichi Matsumoto, Yuishin Izumi, Etsuro Ohta, Fumiya Obata, Ryuji Kaji, Haruhisa Inoue
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 20-year-old dystonia patient with a GCH1 mutation (DYT5). Episomal vectors were used to introduce reprogramming factors (OCT3/4, SOX2, KLF4, L-MYC, LIN28, and p53 carboxy-terminal dominant-negative fragment) to the PBMCs. The generated iPSCs expressed pluripotency markers, and were capable of differentiating into derivates of all three germ layers in vitro. The iPSC line also showed a normal karyotype and preserved the GCH1 mutation...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29034889/generation-of-a-pig-induced-pluripotent-stem-cell-pipsc-line-from-embryonic-fibroblasts-by-incorporating-lin28-to-the-four-transcriptional-factor-mediated-reprogramming-vsmui001-d
#10
Warunya Chakritbudsabong, Ladawan Sariya, Sirikron Pamonsupornvichit, Rassmeepen Pronarkngver, Somjit Chaiwattanarungruengpaisan, Joao N Ferreira, Piyathip Setthawong, Praopilas Phakdeedindan, Mongkol Techakumphu, Theerawat Tharasanit, Sasitorn Rungarunlert
Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28955403/expression-of-ercc1-bcl-2-lin28a-and-ki-67-as-biomarkers-of-response-to-first-line-platinum-based-chemotherapy-in-patients-with-high-grade-extrapulmonary-neuroendocrine-carcinomas-or-small-cell-lung-cancer
#11
Juliana Florinda de M Rêgo, Raphael Salles Scortegagna de Medeiros, Maria Ignez Braghiroli, Breno Galvão, João Evangelista Bezerra Neto, Rodrigo Ramella Munhoz, Juliana Guerra, Suely Nonogaki, Lidia Kimura, Tulio Eduardo Pfiffer, Gilberto de Castro, Paulo Marcelo Hoff, Duilio Rocha Filho, Frederico Perego Costa, Rachel P Riechelmann
BACKGROUND: Small cell lung cancer (SCLC) and high-grade extrapulmonary neuroendocrine carcinomas (EPNEC) share similar histopathological features and treatment, but outcomes may differ. We evaluated in our study the expression of biomarkers associated with response rate (RR) to chemotherapy and overall survival (OS) for these entities. MATERIALS AND METHODS: This is a multicentre retrospective analysis of advanced EPNEC and SCLC patients treated with platinum-based chemotherapy...
2017: Ecancermedicalscience
https://www.readbyqxmd.com/read/28952926/generation-of-a-human-induced-pluripotent-stem-cell-line-cerai001-a-6-using-episomal-vectors
#12
Raymond C B Wong, Sandy S Hung, Stacey Jackson, Vikrant Singh, Shahnaz Khan, Helena H Liang, Lisa S Kearns, Tu Nguyen, Alison Conquest, Maciej Daniszewski, Alex W Hewitt, Alice Pébay
We report the generation of the hiPSC line CERAi001-A-6 from primary human dermal fibroblasts. Reprogramming was performed using episomal vector delivery of OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA for p53.
July 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28947981/lin28b-facilitates-the-progression-and-metastasis-of-pancreatic-ductal-adenocarcinoma
#13
Yunchao Wang, Jian Li, Shixiang Guo, Yongsheng Ouyang, Liangyu Yin, Songsong Liu, Zhiping Zhao, Jiali Yang, Wenjie Huang, Huan Qin, Xin Zhao, Bing Ni, Huaizhi Wang
Lin28B, a Lin28 homologue, represses the biogenesis of let-7 microRNAs (miRNAs), has a role in tumorigenesis, and is considered a potential therapeutic target for various human malignancies. However, the associations between Lin28B and the clinical features and outcomes of patients with pancreatic ductal adenocarcinoma (PDAC) remain unclear. In this study, we explored the clinical significance of Lin28B in PDAC and its association with metastasis by examining tissues from patients with PDAC and elucidated the molecular mechanisms using PDAC cell lines...
September 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28947139/lncrna-hottip-modulates-cancer-stem-cell-properties-in-human-pancreatic-cancer-by-regulating-hoxa9
#14
Zhiqiang Fu, Changhao Chen, Quanbo Zhou, Yinxue Wang, Yue Zhao, Xiaohui Zhao, Wenzhu Li, Shangyou Zheng, Huilin Ye, Lin Wang, Zhanghai He, Qing Lin, Zhihua Li, Rufu Chen
Our previous study demonstrated that long non-coding RNA (lncRNA) HOTTIP was maximally expressed in PDAC, and promoted cancer cell progression and epithelial to mesenchymal transition (EMT). Numerous studies indicated that lncRNAs or EMT supported cancer stem cells. However, the role of HOTTIP in pancreatic cancer stem cells (PCSCs) remains unclear. Here, we evaluated the role and mechanism of HOTTIP in PCSCs. First, we analyzed the relationship between HOTTIP expression and overall or disease-free survival in 90 patients with PDAC after radical resection...
September 22, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28945502/comparative-analysis-of-lin28-rna-binding-sites-identified-at-single-nucleotide-resolution
#15
Elizabeth Ransey, Anders Björkbom, Victor S Lelyveld, Przemyslaw Biecek, Lorena Pantano, Jack W Szostak, Piotr Sliz
It remains a formidable challenge to characterize the diverse complexes of RNA binding proteins and their targets. While crosslink and immunoprecipitation (CLIP) methods are powerful techniques that identify RNA targets on a global scale, the resolution and consistency of these methods is a matter of debate. Here we present a comparative analysis of LIN28-pre-let-7 UV-induced crosslinking using a tandem mass spectrometry (MS/MS) and deep sequencing interrogation of in vitro crosslinked complexes. Interestingly, analyses by the two methods diverge in their identification of crosslinked nucleotide identity - whereas bioinformatics and sequencing analyses suggest guanine in mammalian cells, MS/MS identifies uridine...
September 25, 2017: RNA Biology
https://www.readbyqxmd.com/read/28910332/concurrent-treatment-with-simvastatin-and-nf-%C3%AE%C2%BAb-inhibitor-in-human-castration-resistant-prostate-cancer-cells-exerts-synergistic-anti-cancer-effects-via-control-of-the-nf-%C3%AE%C2%BAb-lin28-let-7-mirna-signaling-pathway
#16
Minyong Kang, Kyoung-Hwa Lee, Hye Sun Lee, Chang Wook Jeong, Ja Hyeon Ku, Hyeon Hoe Kim, Cheol Kwak
We examined the anti-cancer effects and molecular mechanism of simvastatin in human castration-resistant prostate cancer (CRPC) cells, particularly focused on LIN28B and its target molecule, let-7 microRNA (miRNA) among the various target genes of NF-κB. A human CRPC cell line (PC3) was used in the current study. Gene expression patterns were evaluated using real time-PCR and western blot analysis. CCK-8 assay was used for assessing cell viability and proliferation, and a clonogenic assay was adopted to evaluate clonal proliferative capabilities...
2017: PloS One
https://www.readbyqxmd.com/read/28898112/functional-odontoblastic-like-cells-derived-from-human-ipscs
#17
H Xie, N Dubey, W Shim, C J A Ramachandra, K S Min, T Cao, V Rosa
The induced pluripotent stem cells (iPSCs) have an intrinsic capability for indefinite self-renewal and large-scale expansion and can differentiate into all types of cells. Here, we tested the potential of iPSCs from dental pulp stem cells (DPSCs) to differentiate into functional odontoblasts. DPSCs were reprogrammed into iPSCs via electroporation of reprogramming factors OCT-4, SOX2, KLF4, LIN28, and L-MYC. The iPSCs presented overexpression of the reprogramming genes and high protein expressions of alkaline phosphatase, OCT4, and TRA-1-60 in vitro and generated tissues from 3 germ layers in vivo...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28892074/replacing-reprogramming-factors-with-antibodies-selected-from-combinatorial-antibody-libraries
#18
Joel W Blanchard, Jia Xie, Nadja El-Mecharrafie, Simon Gross, Sohyon Lee, Richard A Lerner, Kristin K Baldwin
The reprogramming of differentiated cells into induced pluripotent stem cells (iPSCs) is usually achieved by exogenous induction of transcription by factors acting in the nucleus. In contrast, during development, signaling pathways initiated at the membrane induce differentiation. The central idea of this study is to identify antibodies that can catalyze cellular de-differentiation and nuclear reprogramming by acting at the cell surface. We screen a lentiviral library encoding ∼100 million secreted and membrane-bound single-chain antibodies and identify antibodies that can replace either Sox2 and Myc (c-Myc) or Oct4 during reprogramming of mouse embryonic fibroblasts into iPSCs...
October 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28886879/analysis-of-putative-biomarkers-of-undifferentiated-spermatogonia-in-dog-testis
#19
Won-Young Lee, Hyun-Jung Park, Ran Lee, Ji-Heon Lee, Hyunjhung Jhun, Tai-Young Hur, Hyuk Song
Spermatogenesis begins after puberty and continues throughout a male's life, and is regulated by spermatogonial stem cells in the seminiferous tubules. Markers of male germ cells, including undifferentiated spermatogonia to fully developed spermatozoa have been identified in rodents, but not in dogs. In this study, to characterize the markers of undifferentiated spermatogonia, histological and immunohistochemical analyses were performed on pre-pubertal (1-month-old), early pubertal (4-month-old), and post-pubertal (7-month-old) dog testes...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28884008/efficient-method-to-create-integration-free-virus-free-myc-and-lin28-free-human-induced-pluripotent-stem-cells-from-adherent-cells
#20
Anant Kamath, Sara Ternes, Stephen McGowan, Anthony English, Rama Mallampalli, Alan B Moy
AIM: Nonviral induced pluripotent stem cell (IPSC) reprogramming is not efficient without the oncogenes, Myc and Lin28. We describe a robust Myc and Lin28-free IPSC reprogramming approach using reprogramming molecules. METHODS: IPSC colony formation was compared in the presence and absence of Myc and Lin28 by the mixture of reprogramming molecules and episomal vectors. RESULTS: While more colonies were observed in cultures transfected with the aforementioned oncogenes, the Myc and Lin28-free method achieved the same reprogramming efficiency as reports that used these oncogenes...
August 2017: Future Science OA
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