keyword
MENU ▼
Read by QxMD icon Read
search

Metabolites

keyword
https://www.readbyqxmd.com/read/28222414/urinary-steroid-metabolites-in-a-case-of-florid-ectopic-cushing-s-syndrome-and-clinical-correlations
#1
Angelos Kyriacou, Karolina G Stepien, Basil Issa
A 51-year old woman was admitted with multiple cerebral, pulmonary and intra-abdominal abscesses. The combination of apparent immunosuppression, obesity, diabetes mellitus, hypertension, hypokalaemia, osteoporotic fractures and bilateral shoulder avascular necrosis led to a clinical diagnosis of Cushing's syndrome (CS). This was biochemically confirmed as follows: midnight serum cortisol 4275 nmol/L (60-250), non-suppressed overnight dexamethasone suppression test, raised salivary cortisol 716 nmol/L (5-46) and ACTH 639 ng/L (0-46)...
October 2016: Hormones: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28222186/alpk1-controls-tifa-traf6-dependent-innate-immunity-against-heptose-1-7-bisphosphate-of-gram-negative-bacteria
#2
Milica Milivojevic, Anne-Sophie Dangeard, Christoph Alexander Kasper, Therese Tschon, Mario Emmenlauer, Claudine Pique, Pamela Schnupf, Julie Guignot, Cécile Arrieumerlou
During infection by invasive bacteria, epithelial cells contribute to innate immunity via the local secretion of inflammatory cytokines. These are directly produced by infected cells or by uninfected bystanders via connexin-dependent cell-cell communication. However, the cellular pathways underlying this process remain largely unknown. Here we perform a genome-wide RNA interference screen and identify TIFA and TRAF6 as central players of Shigella flexneri and Salmonella typhimurium-induced interleukin-8 expression...
February 21, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28222171/metabolite-profiling-of-the-carnivorous-pitcher-plants-darlingtonia-and-sarracenia
#3
Hannu Hotti, Peddinti Gopalacharyulu, Tuulikki Seppänen-Laakso, Heiko Rischer
Sarraceniaceae is a New World carnivorous plant family comprising three genera: Darlingtonia, Heliamphora, and Sarracenia. The plants occur in nutrient-poor environments and have developed insectivorous capability in order to supplement their nutrient uptake. Sarracenia flava contains the alkaloid coniine, otherwise only found in Conium maculatum, in which its biosynthesis has been studied, and several Aloe species. Its ecological role and biosynthetic origin in S. flava is speculative. The aim of the current research was to investigate the occurrence of coniine in Sarracenia and Darlingtonia and to identify common constituents of both genera, unique compounds for individual variants and floral scent chemicals...
2017: PloS One
https://www.readbyqxmd.com/read/28222104/how-important-is-thermodynamics-for-identifying-elementary-flux-modes
#4
Sabine Peres, Mario Jolicœur, Cécile Moulin, Philippe Dague, Stefan Schuster
We present a method for computing thermodynamically feasible elementary flux modes (tEFMs) using equilibrium constants without need of internal metabolite concentrations. The method is compared with the method based on a binary distinction between reversible and irreversible reactions. When all reactions are reversible, adding the constraints based on equilibrium constants reduces the number of elementary flux modes (EFMs) by a factor of two. Declaring in advance some reactions as irreversible, based on reliable biochemical expertise, can in general reduce the number of EFMs by a greater factor...
2017: PloS One
https://www.readbyqxmd.com/read/28222021/intense-blue-light-therapy-during-the-night-time-does-not-suppress-the-rhythmic-melatonin-biosynthesis-in-a-young-boy
#5
K Stebelova, J Kosnacova, M Zeman
OBJECTIVE: Melatonin is a hormone predominantly synthesized and secreted during the night by the pineal gland. Artificial light at night, especially its blue part, acutely suppresses the melatonin production. Th e aim of the present study was to find out, whether an intense blue light phototherapy of severe hyperbilirubinemia, may suppress the melatonin production during the night when the eyes will be properly protected by a sleep mask. METHODS: The main melatonin metabolite, 6-sulphatoxymelatonin was measured in urine in a nine-year old boy suffering from the Crigler-Najjar syndrome type I...
January 1, 2017: Endocrine Regulations
https://www.readbyqxmd.com/read/28222020/high-dose-biotin-therapy-leading-to-false-biochemical-endocrine-profiles-validation-of-a-simple-method-to-overcome-biotin-interference
#6
Marie-Liesse Piketty, Dominique Prie, Frederic Sedel, Delphine Bernard, Claude Hercend, Philippe Chanson, Jean-Claude Souberbielle
BACKGROUND: High-dose biotin therapy is beneficial in progressive multiple sclerosis (MS) and is expected to be adopted by a large number of patients. Biotin therapy leads to analytical interference in many immunoassays that utilize streptavidin-biotin capture techniques, yielding skewed results that can mimic various endocrine disorders. We aimed at exploring this interference, to be able to remove biotin and avoid misleading results. METHODS: We measured free triiodothyronine (fT3), free thyroxine (fT4), thyroid-stimulating hormone (TSH), parathyroid homrone (PTH), 25-hydroxyvitamin D (25OHD), follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, C-peptide, cortisol (Roche Diagnostics assays), biotin and its main metabolites (liquid chromatography tandem mass spectrometry) in 23 plasmas from MS patients and healthy volunteers receiving high-dose biotin, and in 39 biotin-unsupplemented patients, before and after a simple procedure (designated N5) designed to remove biotin by means of streptavidin-coated microparticles...
February 21, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28221941/the-hap-complex-governs-fumonisin-biosynthesis-and-maize-kernel-pathogenesis-in-fusarium-verticillioides
#7
John B Ridenour, Jonathon E Smith, Burton H Bluhm
Contamination of maize ( Zea mays ) with fumonisins produced by the fungus Fusarium verticillioides is a global concern for food safety. Fumonisins are a group of polyketide-derived secondary metabolites linked to esophageal cancer and neural tube birth defects in humans and numerous toxicoses in livestock. Despite the importance of fumonisins in global maize production, the regulation of fumonisin biosynthesis during kernel pathogenesis is poorly understood. The HAP complex is a conserved, heterotrimeric transcriptional regulator that binds the consensus sequence CCAAT to modulate gene expression...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221813/transport-glucuronidation-classification-system-and-pbpk-modeling-new-approach-to-predict-the-impact-of-transporters-on-disposition-of-glucuronides
#8
Shufan Ge, Yingjie Wei, Taijun Yin, Beibei Xu, Song Gao, Ming Hu
Glucuronide metabolites require the action of efflux transporters to exit cells due to their hydrophilic properties. In this study, we proposed a transport-glucuronidation classification system and developed a PBPK model to predict the impact of BCRP on systemic exposure of glucuronides. The clearance by UGTs in S9 fractions and the efflux clearance of glucuronides by BCRP in human UGT1A9-overexpressing HeLa cells were incorporated in the classification system and PBPK model. Based on simulations for glucuronide AUC for theoretical compounds in the classification system, it was indicated that BCRP was more important for compounds with greater efflux clearance of their glucuronides by BCRP regardless of differences in clearance by UGTs...
February 21, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28221792/itraq-based-quantitative-proteomic-analysis-of-germinating-barley-under-ga-and-aba-treatments
#9
Yuqing Huang, Shengguan Cai, Jianbin Zeng, Dezhi Wu, Guoping Zhang
The degradation of starch in barley grains is a primary step of beer production. The addition of appropriate amount of GA promotes the production of fermentable sugars, beneficial to brewing industry. However, the response of proteomics in germinating barley to GA and ABA treatments is not thoroughly understood. In this study, iTRAQ (isobaric tags for relative and absolute quantitation) proteomics analysis was performed to illustrate the change of proteins in Tibetan wild barley XZ72 and XZ95 under GA and ABA treatments during germination...
February 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28221784/development-of-a-biosensor-concept-to-detect-the-production-of-cluster-specific-secondary-metabolites
#10
Yi-Qian Sun, Tobias Busche, Christian Rückert, Constanze Paulus, Yuriy Rebets, Renata Novakova, Joern Kalinowski, Andriy Luzhetskyy, Jan Kormanec, Olga N Sekurova, Sergey B Zotchev
Genome mining of actinomycete bacteria aims at the discovery of novel bioactive secondary metabolites that can be developed into drugs. A new repressor-based biosensor to detect activated secondary metabolite biosynthesis gene clusters in Streptomyces was developed. Biosynthetic gene clusters for undecylprodigiosin and coelimycin in the genome of Streptomyces lividans TK24, which encoded TetR-like repressors and appeared to be almost "silent" based on the RNA-seq data were chosen for the proof-of-principle studies...
February 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28221327/changes-in-the-lipidomic-profile-of-aqueous-humor-in-open-angle-glaucoma
#11
Javier Cabrerizo, Javier A Urcola, Elena Vecino
PURPOSE: To identify and determine differences in lipid profiles of aqueous humor (AH) in patients with open-angle glaucoma (OAG). MATERIALS AND METHODS: The lipidomic profile of 10 samples of AH of patients with OAG and 10 controls was analyzed. Patients with a history of anterior segment surgery, anterior segment pathology, or intraocular injections were excluded. Topical ocular medications within the last 6 months were reported. AH was obtained during the first step of nonperforating deep sclerotomy in OAG patients and during refractive lensectomy in controls...
February 17, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/28221024/imet-a-network-based-computational-tool-to-assist-in-the-annotation-of-metabolites-from-tandem-mass-spectra
#12
Antoni Aguilar-Mogas, Marta Sales-Pardo, Miriam Navarro, Roger Guimerà, Oscar Yanes
Structural annotation of metabolites relies mainly on tandem mass spectrometry (MS/MS) analysis. However, approximately 90% of the known metabolites reported in metabolomic databases do not have annotated spectral data from standards. This situation has fostered the development of computational tools that predict fragmentation patterns in silico and compare these to experimental MS/MS spectra. However, because such methods require the molecular structure of the detected compound to be available for the algorithm, the identification of novel metabolites in organisms relevant for biotechnological and medical applications remains a challenge...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28221022/quantitative-13c-traces-of-glucose-fate-in-hepatitis-b-virus-infected-hepatocytes
#13
Qianfen Wan, Yulan Wang, Huiru Tang
Quantitative characterization of 13C-labeled metabolites is an important part of the stable isotope tracing method widely used in metabolic flux analysis. Due to long relaxation time and low sensitivity of 13C nuclei, direct measurement of 13C labeled metabolites using one dimensional 13C NMR often fails to meet the demand of metabolomics studies especially with large number of samples and metabolites having low abundance. Although HSQC-based 2D NMR methods have improved sensitivity with inversion detection, they are time-consuming thus unsuitable for high-throughput absolute quantification of 13C-labeled metabolites...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28221021/probing-cellular-molecules-with-polya-based-engineered-aptamer-nanobeacon
#14
Lizhen Chen, Jie Chao, Xiangmeng Qu, Hongbo Zhang, Dan Zhu, Shao Su, Ali Aldalbahi, Lianhui Wang, Hao Pei
Adenosine triphosphate (ATP) is a central metabolite that is of critical importance in many cellular processes. The development of sensitive and selective methods for the detection of ATP level in vivo is crucial in diagnostic and theranostic applications. In this work, we have developed a polyA-based aptamer nanobeacon (PAaptNB) with improved efficiency and speed of ATP analysis. We found that the dissociation constants and competitive binding kinetics of the PAaptNB could be programmably regulated by adjusting the polyA length...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28220994/positional-enrichment-by-proton-analysis-pepa-a-one-dimensional-1-h-nmr-approach-for-13-c-stable-isotope-tracer-studies-in-metabolomics
#15
Maria Vinaixa, Miguel A Rodríguez, Suvi Aivio, Jordi Capellades, Josep Gómez, Nicolau Canyellas, Travis H Stracker, Oscar Yanes
A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of (13) C-satellite peaks using 1D-(1) H-NMR spectra. In comparison with (13) C-NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of (13) C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment...
February 21, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28220904/recent-advances-in-occupational-and-environmental-health-hazards-of-workers-exposed-to-gasoline-compounds
#16
REVIEW
Christopher E Ekpenyong, Asuquo E Asuquo
The impact of health and environmental hazards, associated with the constituents of gasoline, on occupationally exposed workers has been recorded over the past few decades. However, the scientific literature on their pathogenic potential remains incomplete, which could affect the current understanding of the associated health risks. This review provides current information based on recently improved research techniques to evaluate gasoline toxicity profiles for humans. Our current knowledge provides insight into the intricate mechanism of gasoline-induced adverse effects, including the formation of reactive metabolites via bio-activation and subsequent generation of reactive oxygen species (ROS) and oxidative stress, which are involved in multiple mechanisms that are central to the aetiology of gasoline-induced toxicity...
February 21, 2017: International Journal of Occupational Medicine and Environmental Health
https://www.readbyqxmd.com/read/28220901/distinct-signatures-of-dental-plaque-metabolic-byproducts-dictated-by-periodontal-inflammatory-status
#17
Akito Sakanaka, Masae Kuboniwa, Ei Hashino, Takeshi Bamba, Eiichiro Fukusaki, Atsuo Amano
Onset of chronic periodontitis is associated with an aberrant polymicrobial community, termed dysbiosis. Findings regarding its etiology obtained using high-throughput sequencing technique suggested that dysbiosis holds a conserved metabolic signature as an emergent property. The purpose of this study was to identify robust biomarkers for periodontal inflammation severity. Furthermore, we investigated disease-associated metabolic signatures of periodontal microbiota using a salivary metabolomics approach. Whole saliva samples were obtained from adult subjects before and after removal of supragingival plaque (debridement)...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220878/homozygous-carriers-of-the-tcf7l2-rs7903146-t-allele-show-altered-postprandial-response-in-triglycerides-and-triglyceride-rich-lipoproteins
#18
L Engelbrechtsen, T H Hansen, Y Mahendran, P Pyl, E Andersson, A Jonsson, A Gjesing, A Linneberg, T Jørgensen, T Hansen, H Vestergaard
The TCF7L2 rs7903146 T-allele shows the strongest association with type 2 diabetes (T2D) among common gene variants. The aim of this study was to assess circulating levels of metabolites following a meal test in individuals carrying the high risk rs790346 TT genotype (cases) and low-risk CC genotype (controls). Sixty-two men were recruited based on TCF7L2 genotype, 31 were TT carriers and 31 were age- and BMI-matched CC carriers. All participants consumed a test meal after 12 hours of fasting. Metabolites were measured using proton nuclear magnetic resonance (NMR) spectroscopy...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220762/drug-detection-in-breath-non-invasive-assessment-of-illicit-or-pharmaceutical-drugs
#19
Phillip Trefz, Svend Kamysek, Patricia Fuchs, Pritam Sukul, Jochen Schubert, Wolfram Miekisch
Breath analysis not only holds great potential for the development of new non-invasive diagnostic methods, but also for the identification and follow up of drug levels in breath. This is of interest for both, forensic and medical science. On the one hand, the detection of drugs of abuse in exhaled breath - similar to the well-known breath alcohol tests - would be highly desirable as an alternative to blood or urine analysis in situations such as police controls for drugged driving. The noninvasive detection of drugs and their metabolites is thus of great interest in forensic science, especially since marijuana is becoming legalized in certain parts of the U...
February 21, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28220690/metabolite-labelling-as-a-tool-to-define-hierarchies-in-clostridium-acetobutylicum-sugar-usage-and-its-relevance-for-biofuel-production
#20
María Hidalgo, Elena Puerta-Fernández
No abstract text is available yet for this article.
February 21, 2017: Microbial Biotechnology
keyword
keyword
56332
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"