keyword
MENU ▼
Read by QxMD icon Read
search

virus cell entry

keyword
https://www.readbyqxmd.com/read/28943338/structure-of-a-reptilian-adenovirus-reveals-a-phage-tailspike-fold-stabilizing-a-vertebrate-virus-capsid
#1
Rosa Menéndez-Conejero, Thanh H Nguyen, Abhimanyu K Singh, Gabriela N Condezo, Rachel E Marschang, Mark J van Raaij, Carmen San Martín
Although non-human adenoviruses (AdVs) might offer solutions to problems posed by human AdVs as therapeutic vectors, little is known about their basic biology. In particular, there are no structural studies on the complete virion of any AdV with a non-mammalian host. We combine mass spectrometry, cryo-electron microscopy, and protein crystallography to characterize the composition and structure of a snake AdV (SnAdV-1, Atadenovirus genus). SnAdV-1 particles contain the genus-specific proteins LH3, p32k, and LH2, a previously unrecognized structural component...
September 6, 2017: Structure
https://www.readbyqxmd.com/read/28939853/rsv-glycoprotein-and-genomic-rna-dynamics-reveal-filament-assembly-prior-to-the-plasma-membrane
#2
Daryll Vanover, Daisy V Smith, Emmeline L Blanchard, Eric Alonas, Jonathan L Kirschman, Aaron W Lifland, Chiara Zurla, Philip J Santangelo
The human respiratory syncytial virus G protein plays an important role in the entry and assembly of filamentous virions. Here, we report the use of fluorescently labeled soybean agglutinin to selectively label the respiratory syncytial virus G protein in living cells without disrupting respiratory syncytial virus infectivity or filament formation and allowing for interrogations of respiratory syncytial virus virion assembly. Using this approach, we discovered that plasma membrane-bound respiratory syncytial virus G rapidly recycles from the membrane via clathrin-mediated endocytosis...
September 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28939750/inhibition-of-ebv-mediated-membrane-fusion-by-anti-ghgl-antibodies
#3
Karthik Sathiyamoorthy, Jiansen Jiang, Britta S Möhl, Jia Chen, Z Hong Zhou, Richard Longnecker, Theodore S Jardetzky
Herpesvirus entry into cells requires the coordinated action of multiple virus envelope glycoproteins, including gH, gL, and gB. For EBV, the gp42 protein assembles into complexes with gHgL heterodimers and binds HLA class II to activate gB-mediated membrane fusion with B cells. EBV tropism is dictated by gp42 levels in the virion, as it inhibits entry into epithelial cells while promoting entry into B cells. The gHgL and gB proteins are targets of neutralizing antibodies and potential candidates for subunit vaccine development, but our understanding of their neutralizing epitopes and the mechanisms of inhibition remain relatively unexplored...
September 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28939581/protocol-of-a-randomised-controlled-trial-characterising-the-immune-responses-induced-by-varicella-zoster-virus-vzv-vaccination-in-healthy-kenyan-women-setting-the-stage-for-a-potential-vzv-based-hiv-vaccine
#4
Catia T Perciani, Walter Jaoko, Sharon Walmsley, Bashir Farah, Salaheddin M Mahmud, Mario Ostrowski, Omu Anzala, Kavi-Icr Team, Kelly S MacDonald
INTRODUCTION: A protective HIV vaccine would be expected to induce durable effector immune responses at the mucosa, restricting HIV infection at its portal of entry. We hypothesise that use of varicella-zoster virus (VZV) as an HIV delivery vector could generate sustained and robust tissue-based immunity against HIV antigens to provide long-term protection against HIV. Given that HIV uniquely targets immune-activated T cells, the development of human vaccines against HIV must also involve a specific examination of the safety of the vector...
September 21, 2017: BMJ Open
https://www.readbyqxmd.com/read/28932658/the-process-of-wrapping-virus-revealed-by-a-force-tracing-technique-and-simulations
#5
Yangang Pan, Fuxian Zhang, Liuyang Zhang, Shuheng Liu, Mingjun Cai, Yuping Shan, Xianqiao Wang, Hanzhong Wang, Hongda Wang
Viral entry into the host cell is the first step of virus infection; however, its dynamic process via endocytosis remains largely elusive. Here, the force tracing technique and single particle simulation are combined to investigate the invagination of single human enterovirus 71 (HEV71, a positive single-stranded RNA virus that is associated with hand, foot, and mouth disease) via cell membranes during its host cell entry. The experimental results reveal that the HEV71 invaginates in membrane vesicles at a force of 58 ± 16 pN, a duration time of 278 ± 68 ms...
September 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28924154/a-dna-aptamer-efficiently-inhibits-the-infectivity-of-bovine-herpesvirus-1-by-blocking-viral-entry
#6
Jian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen, Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E Sealy, Munir Iqbal, Yongqing Li
Bovine herpesvirus 1 (BoHV-1) is an important pathogen of domestic and wild cattle responsible for major economic losses in dairy and beef industries throughout the world. Inhibition of viral entry plays a crucial role in the control of BoHV-1 infection and aptamers have been reported to inhibit viral replication. In this study, nine DNA aptamers that target BoHV-1 were generated using systemic evolution of ligands by exponential enrichment. Of the nine candidates, aptamer IBRV-A4 exhibited the highest affinity and specificity for BoHV-1, which bound to BoHV-1 with a Kd value of 3...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28923942/identification-of-sialic-acid-binding-function-for-the-middle-east-respiratory-syndrome-coronavirus-spike-glycoprotein
#7
Wentao Li, Ruben J G Hulswit, Ivy Widjaja, V Stalin Raj, Ryan McBride, Wenjie Peng, W Widagdo, M Alejandra Tortorici, Brenda van Dieren, Yifei Lang, Jan W M van Lent, James C Paulson, Cornelis A M de Haan, Raoul J de Groot, Frank J M van Kuppeveld, Bart L Haagmans, Berend-Jan Bosch
Middle East respiratory syndrome coronavirus (MERS-CoV) targets the epithelial cells of the respiratory tract both in humans and in its natural host, the dromedary camel. Virion attachment to host cells is mediated by 20-nm-long homotrimers of spike envelope protein S. The N-terminal subunit of each S protomer, called S1, folds into four distinct domains designated S1(A) through S1(D) Binding of MERS-CoV to the cell surface entry receptor dipeptidyl peptidase 4 (DPP4) occurs via S1(B) We now demonstrate that in addition to DPP4, MERS-CoV binds to sialic acid (Sia)...
September 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28918745/coupling-of-translation-initiation-and-termination-does-not-depend-on-the-mode-of-initiation
#8
E A Sogorin, G K Selikhanov, S Ch Agalarov
Recently we described a novel phenomenon observed during eukaryotic translation in a cell-free system: the coupling of initiation and termination on different mRNA molecules. Here we show that the phenomenon does not depend on a special mode of initiation. The mRNAs with certain leader sequences known to require different determinants for successful initiation were examined. Even in a case of using the intergenic internal ribosome entry site (IRES) of cricket paralysis virus RNA as the leader sequence, while no initiation factors are required, the effect of coupling is well expressed, including trials in the presence of hippuristanol as an inhibitor of eIF4A...
July 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28911115/the-ires5-utr-of-the-dicistrovirus-cricket-paralysis-virus-is-a-type-iii-ires-containing-an-essential-pseudoknot-structure
#9
Lauriane Gross, Quentin Vicens, Evelyne Einhorn, Audrey Noireterre, Laure Schaeffer, Lauriane Kuhn, Jean-Luc Imler, Gilbert Eriani, Carine Meignin, Franck Martin
Cricket paralysis virus (CrPV) is a dicistrovirus. Its positive-sense single-stranded RNA genome contains two internal ribosomal entry sites (IRESs). The 5' untranslated region (5'UTR) IRES5'UTR mediates translation of non-structural proteins encoded by ORF1 whereas the well-known intergenic region (IGR) IRESIGR is required for translation of structural proteins from open reading frame 2 in the late phase of infection. Concerted action of both IRES is essential for host translation shut-off and viral translation...
September 6, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28904550/maoto-a-traditional-japanese-herbal-medicine-inhibits-uncoating-of-influenza-virus
#10
Shinta Masui, Shigeki Nabeshima, Kazuhiko Ajisaka, Kei Yamauchi, Ryota Itoh, Kazunari Ishii, Toshinori Soejima, Kenji Hiromatsu
We previously reported in randomized controlled trials that maoto, a traditional herbal medicine, showed clinical and virological efficacy for seasonal influenza. In this study, a culturing system for influenza was used to test the effect of maoto. A549 cells in the culture were infected with influenza virus A (PR8) and followed after treatment with maoto; the virus titers in the culture supernatant, intracellular viral proteins, and viral RNA were determined. When infected cells were cultured with maoto for 24 hr, the virus titer and protein were significantly reduced compared with medium only...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28904344/perturbed-cholesterol-and-vesicular-trafficking-associated-with-dengue-blocking-in-wolbachia-infected-aedes-aegypti-cells
#11
Vincent Geoghegan, Kirsty Stainton, Stephanie M Rainey, Thomas H Ant, Adam A Dowle, Tony Larson, Svenja Hester, Philip D Charles, Benjamin Thomas, Steven P Sinkins
Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control. To better understand the mechanisms of virus inhibition, we here perform proteomic quantification of the effects of Wolbachia in Aedes aegypti mosquito cells and midgut. Perturbations are observed in vesicular trafficking, lipid metabolism and in the endoplasmic reticulum that could impact viral entry and replication...
September 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28904199/calcitonin-gene-related-peptide-induces-hiv-1-proteasomal-degradation-in-mucosal-langerhans-cells
#12
Morgane Bomsel, Yonatan Ganor
The neuro-immune dialogue between peripheral neurons and Langerhans cells (LCs) within mucosal epithelia protects against incoming pathogens. LCs rapidly internalize human immunodeficiency virus type 1 (HIV-1) upon its sexual transmission and then trans-infect CD4+ T-cells. We recently found that the neuropeptide calcitonin gene-related peptide (CGRP), secreted mucosally from peripheral neurons, inhibits LCs-mediated HIV-1 trans-infection. Herein, we investigated the mechanism of CGRP-induced inhibition, focusing on HIV-1 degradation in LCs and its interplay with trans-infection...
September 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28903753/acquisition-of-resistance-to-avian-leukosis-virus-subgroup-b-through-mutations-on-tvb-cysteine-rich-domains-in-df-1-chicken-fibroblasts
#13
Hong Jo Lee, Kyung Youn Lee, Young Hyun Park, Hee Jung Choi, Yongxiu Yao, Venugopal Nair, Jae Yong Han
Avian leukosis virus (ALV) is a retrovirus that causes tumors in avian species, and its vertical and horizontal transmission in poultry flocks results in enormous economic losses. Despite the discovery of specific host receptors, there have been few reports on the modulation of viral susceptibility via genetic modification. We therefore engineered acquired resistance to ALV subgroup B using CRISPR/Cas9-mediated genome editing technology in DF-1 chicken fibroblasts. Using this method, we efficiently modified the tumor virus locus B (tvb) gene, encoding the TVB receptor, which is essential for ALV subgroup B entry into host cells...
September 13, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28903577/a-2-4-amino-acid-deletion-in-the-v5-region-of-hiv-1-env-gp120-confers-viral-resistance-to-the-broadly-neutralizing-human-monoclonal-antibody-vrc01
#14
Shingo Tachibana, Maho Sasaki, Takako Tanaka, Mari Inoue, Youdiil Ophinni, Tomohiro Kotaki, Masanori Kameoka
The envelope glycoprotein (Env) gp120 of human immunodeficiency virus type 1 (HIV-1) plays a critical role in viral entry into host cells. The broadly neutralizing human monoclonal antibody VRC01, which recognizes the CD4 binding site on gp120, neutralizes more than 90% of HIV-1 isolates. However, some of the CRF01_AE viruses prevalent in Southeast Asia are resistant to VRC01-mediated neutralization. We previously reported that 3 amino acid residues at positions 185, 186, and 197 of gp120 played an important role in the VRC01 resistance of CRF01_AE Env (AE-Env) clones isolated from HIV-infected Thai individuals...
September 13, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28903466/doxycycline-enhances-adsorption-and-inhibits-early-stage-replication-of-porcine-reproductive-and-respiratory-syndrome-virus-in-vitro
#15
Yuming Li, Zhuanchang Wu, Ke Liu, Pengfei Qi, Jinpeng Xu, Jianchao Wei, Beibei Li, Donghua Shao, Yuanyuan Shi, Yafeng Qiu, Zhiyong Ma
Porcine reproductive and respiratory syndrome virus (PRRSV) has an important impact on the pig industry. Doxycycline (Dox) is a second-generation tetracycline widely used for treating bacterial infections. We evaluated the antiviral effect of Dox against PRRSV infection in Marc-145 cells that were susceptible to PRRSV infection. Dox significantly reduced the PRRSV-induced cytopathic effect and effectively restrained PRRSV replication in a dose-dependent manner. The 50% effective concentration of Dox was approximately 0...
September 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28903457/the-lifecycle-of-the-ebola-virus-in-host-cells
#16
REVIEW
Dong-Shan Yu, Tian-Hao Weng, Xiao-Xin Wu, Frederick X C Wang, Xiang-Yun Lu, Hai-Bo Wu, Nan-Ping Wu, Lan-Juan Li, Hang-Ping Yao
Ebola haemorrhagic fever causes deadly disease in humans and non-human primates resulting from infection with the Ebola virus (EBOV) genus of the family Filoviridae. However, the mechanisms of EBOV lifecycle in host cells, including viral entry, membrane fusion, RNP formation, GP-tetherin interaction, and VP40-inner leaflet association remain poorly understood. This review describes the biological functions of EBOV proteins and their roles in the lifecycle, summarizes the factors related to EBOV proteins or RNA expression throughout the different phases, and reviews advances with regards to the molecular events and mechanisms of the EBOV lifecycle...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28903311/development-of-hepatoma-derived-bidirectional-oval-like-cells-as-a-model-to-study-host-interactions-with-hepatitis-c-virus-during-differentiation
#17
Masahiko Ito, Suofeng Sun, Takasuke Fukuhara, Ryosuke Suzuki, Miho Tamai, Toyohiko Yamauchi, Kenji Nakashima, Yoh-Ichi Tagawa, Shigetoshi Okazaki, Yoshiharu Matsuura, Takaji Wakita, Tetsuro Suzuki
Directed differentiation of human stem cells including induced pluripotent stem cells into hepatic cells potentially leads to acquired susceptibility to hepatitis C virus (HCV) infection. However, cellular determinants that change their expression during cell reprogramming or hepatic differentiation and are pivotal for supporting the HCV life cycle remain unclear. In this study, by introducing a set of reprogramming factors, we established HuH-7-derived oval-like cell lines, Hdo-17 and -23, which possess features of bipotential liver precursors...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28899754/on-the-whereabouts-of-hiv-1-cellular-entry-and-its-fusion-ports
#18
REVIEW
G Maria Jakobsdottir, Maro Iliopoulou, Rory Nolan, Luis Alvarez, Alex A Compton, Sergi Padilla-Parra
HIV-1 disseminates to diverse tissues through different cell types and establishes long-lived reservoirs. The exact cellular compartment where fusion occurs differs depending on the cell type and mode of viral transmission. This implies that HIV-1 may modulate a number of common host cell factors in different cell types. In this review, we evaluate recent advances on the host cell factors that play an important role in HIV-1 entry and fusion. New insights from restriction factors inhibiting virus-cell fusion in vitro may contribute to the development of future therapeutic interventions...
September 9, 2017: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/28899753/precise-gene-editing-of-chicken-na-h-exchange-type-1-chnhe1-confers-resistance-to-avian-leukosis-virus-subgroup-j-alv-j
#19
Hong Jo Lee, Kyung Youn Lee, Kyung Min Jung, Kyung Je Park, Ko On Lee, Jeong-Yong Suh, Yongxiu Yao, Venugopal Nair, Jae Yong Han
Avian leukosis virus subgroup J (ALV-J), first isolated in the late 1980s, has caused economic losses to the poultry industry in many countries. As all chicken lines studied to date are susceptible to ALV infection, there is enormous interest in developing resistant chicken lines. The ALV-J receptor, chicken Na(+)/H(+) exchange 1 (chNHE1) and the critical amino acid sequences involved in viral attachment and entry have already been characterized. However, there are no reported attempts to induce resistance to the virus by targeted genome modification of the receptor sequences...
September 9, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28893938/epstein-barr-virus-associated-lymphomas
#20
REVIEW
Claire Shannon-Lowe, Alan B Rickinson, Andrew I Bell
Epstein-Barr virus (EBV), originally discovered through its association with Burkitt lymphoma, is now aetiologically linked to a remarkably wide range of lymphoproliferative lesions and malignant lymphomas of B-, T- and NK-cell origin. Some occur as rare accidents of virus persistence in the B lymphoid system, while others arise as a result of viral entry into unnatural target cells. The early finding that EBV is a potent B-cell growth transforming agent hinted at a simple oncogenic mechanism by which this virus could promote lymphomagenesis...
October 19, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
keyword
keyword
56323
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"