keyword
MENU ▼
Read by QxMD icon Read
search

virus cell entry

keyword
https://www.readbyqxmd.com/read/28713784/identification-of-novel-and-efficacious-chemical-compounds-that-disturb-influenza-a-virus-entry-in-vitro
#1
Hany Khalil, Tamer El Malah, Ahmed I Abd El Maksoud, Ibrahim El Halfawy, Ahmed A El Rashedy, Mahmoud El Hefnawy
Influenza A virus is a negative RNA stranded virus of the family Orthomyxoviridae, and represents a major public health threat, compounding existing disease conditions. Influenza A virus replicates rapidly within its host and the segmented nature of its genome facilitates re-assortment, whereby whole genes are exchanged between influenza virus subtypes during replication. Antiviral medications are important pharmacological tools in influenza virus prophylaxis and therapy. However, the use of currently available antiviral is impeded by sometimes high levels of resistance in circulating virus strains...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28708602/development-of-hepatoma-derived-bidirectional-oval-like-cells-as-a-model-to-study-host-interactions-with-hepatitis-c-virus-during-differentiation
#2
Masahiko Ito, Suofeng Sun, Takasuke Fukuhara, Ryosuke Suzuki, Miho Tamai, Toyohiko Yamauchi, Kenji Nakashima, Yoh-Ichi Tagawa, Shigetoshi Okazaki, Yoshiharu Matsuura, Takaji Wakita, Tetsuro Suzuki
Directed differentiation of human stem cells including induced pluripotent stem cells into hepatic cells potentially leads to acquired susceptibility to hepatitis C virus (HCV) infection. However, cellular determinants that change their expression during cell reprogramming or hepatic differentiation and are pivotal for supporting the HCV life cycle remain unclear. In this study, by introducing a set of reprogramming factors, we established HuH-7-derived oval-like cell lines, Hdo-17 and -23, which possess features of bipotential liver precursors...
July 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28707270/profiling-of-cellular-microrna-responses-during-the-early-stages-of-kshv-infection
#3
Hosni A M Hussein, Shaw M Akula
Kaposi's sarcoma-associated herpesvirus (KSHV) causes a variety of cancers, including Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman disease (MCD). Host cellular microRNAs (miRNAs) play important post-transcriptional regulatory roles in gene expression and can greatly influence virus-host cell interactions. This study investigated cellular miRNA expression profiles operating in response to early stages of KSHV infection of human Burkitt lymphoma B cells (BJAB). We employed deep sequencing to analyze miRNA expression in KSHV-infected BJAB cells 15 min post infection (PI) and compared this to uninfected BJAB cells...
July 13, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28705724/chse-214-a-model-for-studying-extracellular-dsrna-sensing-in-vitro
#4
A Monjo, S J Poynter, S J DeWitte-Orr
Double stranded RNA (dsRNA) is produced by almost all viruses during their replicative cycle and is a potent inducer of the innate antiviral immune response including inducing expression of type I interferons (IFNs) and interferon stimulated genes (ISGs). During lytic virus infections intracellular dsRNA can escape into the extracellular space, where surface pattern recognition receptors, such as class A scavenger receptors (SR-As) facilitate its binding and entry into neighbouring cells. Studying extracellular dsRNA entry is difficult due to the ubiquitous expression profile and compensatory dsRNA binding characteristics of SR-As; a SR-A deficient cell line has yet to be identified...
July 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28705263/antiviral-activity-of-dual-acting-hydrocarbon-stapled-peptides-against-hiv-1-predominantly-circulating-in-china
#5
Yan Wang, Francesca Curreli, Wei Si Xu, Zhen Peng Li, De Sheng Kong, Li Ren, Kun Xue Hong, Shi Bo Jiang, Yi Ming Shao, Asim K Debnath, Li Ying Ma
OBJECTIVE: New rationally designed i,i+7-hydrocarbon-stapled peptides that target both HIV-1 assembly and entry have been shown to have antiviral activity against HIV-1 subtypes circulating in Europe and North America. Here, we aimed to evaluate the antiviral activity of these peptides against HIV-1 subtypes predominantly circulating in China. METHODS: The antiviral activity of three i,i+7-hydrocarbon-stapled peptides, NYAD-36, NYAD-67, and NYAD-66, against primary HIV-1 CRF07_BC and CRF01_AE isolates was evaluated in peripheral blood mononuclear cells (PBMCs)...
June 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28704658/fusion-stage-of-hiv-1-entry-depends-on-virus-induced-cell-surface-exposure-of-phosphatidylserine
#6
Elena Zaitseva, Eugene Zaitsev, Kamran Melikov, Anush Arakelyan, Mariana Marin, Rafael Villasmil, Leonid B Margolis, Gregory B Melikyan, Leonid V Chernomordik
HIV-1 entry into host cells starts with interactions between the viral envelope glycoprotein (Env) and cellular CD4 receptors and coreceptors. Previous work has suggested that efficient HIV entry also depends on intracellular signaling, but this remains controversial. Here we report that formation of the pre-fusion Env-CD4-coreceptor complexes triggers non-apoptotic cell surface exposure of the membrane lipid phosphatidylserine (PS). HIV-1-induced PS redistribution depends on Ca(2+) signaling triggered by Env-coreceptor interactions and involves the lipid scramblase TMEM16F...
July 12, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28704569/a-virulence-associated-filamentous-bacteriophage-of-neisseria-meningitidis-increases-host-cell-colonisation
#7
Emmanuelle Bille, Julie Meyer, Anne Jamet, Daniel Euphrasie, Jean-Philippe Barnier, Terry Brissac, Anna Larsen, Philippe Pelissier, Xavier Nassif
Neisseria meningitidis is a commensal of human nasopharynx. In some circumstances, this bacteria can invade the bloodstream and, after crossing the blood brain barrier, the meninges. A filamentous phage, designated MDAΦ for Meningococcal Disease Associated, has been associated with invasive disease. In this work we show that the prophage is not associated with a higher virulence during the bloodstream phase of the disease. However, looking at the interaction of N. meningitidis with epithelial cells, a step essential for colonization of the nasopharynx, we demonstrate that the presence of the prophage, via the production of viruses, increases colonization of encapsulated meningococci onto monolayers of epithelial cells...
July 13, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28702128/a-novel-host-factor-for-human-respiratory-syncytial-virus
#8
COMMENT
Masfique Mehedi, Peter L Collins, Ursula J Buchholz
Human respiratory syncytial virus (RSV) is the leading viral cause of severe lower respiratory disease in young children worldwide. As part of a genome-wide siRNA screen, we recently discovered that actin-related protein 2 (ARP2) is a host factor in the RSV replication cycle. ARP2 is a major constituent of the ARP2/3 complex, which catalyzes actin polymerization involved in cell morphology and motility. In the course of investigating this finding, we also found that RSV infection of human lung epithelial A459 cells induced filopodia formation and stimulated cell motility...
2017: Communicative & Integrative Biology
https://www.readbyqxmd.com/read/28701394/single-particle-detection-of-transcription-following-rotavirus-entry
#9
Eric N Salgado, Srigokul Upadhyayula, Stephen C Harrison
Infectious rotavirus particles are triple-layered, icosahedral assemblies. The outer layer proteins, VP4 (cleaved to VP8* and VP5*) and VP7, surround a transcriptionally competent, double-layer particle (DLP), which they deliver into the cytosol. During entry of rhesus rotavirus, VP8* interacts with cell-surface gangliosides, allowing engulfment into a membrane vesicle by a clathrin-independent process. Escape into the cytosol and outer-layer shedding depend on interaction of a hydrophobic surface on VP5* with the membrane bilayer and on a large-scale conformational change...
July 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28700571/open-and-closed-structures-reveal-allostery-and-pliability-in-the-hiv-1-envelope-spike
#10
Gabriel Ozorowski, Jesper Pallesen, Natalia de Val, Dmitry Lyumkis, Christopher A Cottrell, Jonathan L Torres, Jeffrey Copps, Robyn L Stanfield, Albert Cupo, Pavel Pugach, John P Moore, Ian A Wilson, Andrew B Ward
For many enveloped viruses, binding to a receptor(s) on a host cell acts as the first step in a series of events culminating in fusion with the host cell membrane and transfer of genetic material for replication. The envelope glycoprotein (Env) trimer on the surface of HIV is responsible for receptor binding and fusion. Although Env can tolerate a high degree of mutation in five variable regions (V1-V5), and also at N-linked glycosylation sites that contribute roughly half the mass of Env, the functional sites for recognition of receptor CD4 and co-receptor CXCR4/CCR5 are conserved and essential for viral fitness...
July 12, 2017: Nature
https://www.readbyqxmd.com/read/28700372/immunohistochemical-demonstration-of-parvovirus-b19-viral-protein-2-in-periflexural-exanthema-in-an-adult-supporting-antibody-dependent-enhancement-as-means-of-endothelial-uptake-of-the-virus
#11
Carlos Santonja, Úrsula Pielasinski, Jorge Polo, Heinz Kutzner, Luis Requena
Human parvovirus B19 (B19V) causes a number of skin exanthemas and has been related to both cutaneous and systemic diseases. Tropism of the virus for the rapidly proliferating erythroid progenitor cells in the bone marrow and fetal liver explains the pathogenesis of anemia and fetal hydrops. The cutaneous lesions of erythema infectiosum and other B19V-related exanthemas have been attributed to the deposition of immune complexes in the skin. We report on the immunohistochemical detection of B19V protein in the cytoplasm of dermal endothelial cells in a case of periflexural exanthema in a 28-year-old woman...
July 6, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28699869/hepatitis-c-virus-in-vitro-replication-is-efficiently-inhibited-by-acridone-fac4
#12
Guilherme Rodrigues Fernandes Campos, Cíntia Bittar, Ana Carolina Gomes Jardim, Jacqueline Farinha Shimizu, Mariana Nogueira Batista, Eder Ramos Paganini, Letícia Ribeiro de Assis, Christopher Bartlett, Mark Harris, Vanderlan da Silva Bolzani, Luis Octavio Regasini, Paula Rahal
Hepatitis C virus (HCV) affects about 170 million people worldwide. The current treatment has a high cost and variable response rates according to the virus genotype. Acridones, a group of compounds extracted from natural sources, showed potential antiviral actions against HCV. Thus, this study aimed to evaluate the effect of a panel of 14 synthetic acridones on the HCV life cycle. The compounds were screened using an Huh7.5 cell line stably harbouring the HCV genotype 2a subgenomic replicon SGR-Feo-JFH-1...
July 12, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28699636/idiosyncratic-m%C3%A3-ji%C3%A4-ng-virus-attachment-glycoprotein-directs-a-host-cell-entry-pathway-distinct-from-genetically-related-henipaviruses
#13
Ilona Rissanen, Asim A Ahmed, Kristopher Azarm, Shannon Beaty, Patrick Hong, Sham Nambulli, W Paul Duprex, Benhur Lee, Thomas A Bowden
In 2012, cases of lethal pneumonia among Chinese miners prompted the isolation of a rat-borne henipavirus (HNV), Mòjiāng virus (MojV). Although MojV is genetically related to highly pathogenic bat-borne henipaviruses, the absence of a conserved ephrin receptor-binding motif in the MojV attachment glycoprotein (MojV-G) indicates a differing host-cell recognition mechanism. Here we find that MojV-G displays a six-bladed β-propeller fold bearing limited similarity to known paramyxoviral attachment glycoproteins, in particular at host receptor-binding surfaces...
July 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28698985/a-cinnamon-derived-procyanidin-type-a-compound-inhibits-hepatitis-c-virus-cell-entry
#14
Catherine Fauvelle, Melanie Lambotin, Laura Heydmann, Ekambaranellore Prakash, Sunil Bhaskaran, Mohan Vishwaraman, Thomas F Baumert, Christiane Moog
BACKGROUND AND AIMS: Chronic hepatitis C virus (HCV) infection is a major cause of liver disease worldwide. Although direct-acting antivirals can cure the large majority of treated patients, important limitations remain, including treatment failure and high costs precluding access to therapy in resource-limited settings. We report herein the anti-HCV effects of IND02, a procyanidin type A molecule, isolated and characterized from cinnamon. METHODS AND RESULTS: Using cellculture-derived HCV (HCVcc), HCV pseudoparticles (HCVpp), and subgenomic replicons, we demonstrated that IND02 markedly and dose-dependently inhibited HCV cell entry...
July 11, 2017: Hepatology International
https://www.readbyqxmd.com/read/28698279/zika-virus-persistently-infects-and-is-basolaterally-released-from-primary-human-brain-microvascular-endothelial-cells
#15
Megan C Mladinich, John Schwedes, Erich R Mackow
Zika virus (ZIKV) is a mosquito-borne Flavivirus that has emerged as the cause of encephalitis and fetal microencephaly in the Americas. ZIKV uniquely persists in human bodily fluids for up to 6 months, is sexually transmitted, and traverses the placenta and the blood-brain barrier (BBB) to damage neurons. Cells that support persistent ZIKV replication and mechanisms by which ZIKV establishes persistence remain enigmatic but central to ZIKV entry into protected neuronal compartments. The endothelial cell (EC) lining of capillaries normally constrains transplacental transmission and forms the BBB, which selectively restricts access of blood constituents to neurons...
July 11, 2017: MBio
https://www.readbyqxmd.com/read/28696795/decoy-receptor-3-down-regulates-centrosomal-protein-70%C3%A2-kda-specifically-in-rheumatoid-synovial-fibroblasts
#16
Koji Fukuda, Yasushi Miura, Toshihisa Maeda, Shinya Hayashi, Ryosuke Kuroda
OBJECTIVES: Decoy receptor 3 (DcR3) competitively binds to Fas ligand, lymphotoxin-related inducible ligand that competes for glycoprotein D binding to herpes virus entry mediator on T cells (LIGHT) and TNF-like ligand 1A (TL1A), thereby preventing their effects. Using a microarray assay, we previously newly identified centrosomal protein 70 kDa (CEP70) as one of the genes whose expression in fibroblast-like synoviocytes from patients with rheumatoid arthritis (RA-FLS) is reduced by DcR3...
July 11, 2017: Modern Rheumatology
https://www.readbyqxmd.com/read/28688086/sequencing-the-biology-of-entry-the-retroviral-env-gene
#17
Ronald Swanstrom, William D Graham, Shuntai Zhou
The surface envelope protein of any virus is major determinant of the host cell that is infected and as a result a major determinant of viral pathogenesis. Retroviruses have a single surface protein named Env. It is a trimer of heterodimers and is responsible for binding to the host cell receptor and mediating fusion between the viral and host membranes. In this review we will discuss the history of the discovery of the avian leukosis virus (ALV) and human immunodeficiency virus type 1 (HIV-1) Env proteins and their receptor specificity, comparing the many differences but having some similarities...
July 9, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28684008/ribosomal-chamber-music-toward-an-understanding-of-ires-mechanisms
#18
REVIEW
Hiroshi Yamamoto, Anett Unbehaun, Christian M T Spahn
Internal initiation is a 5'-end-independent mode of translation initiation engaged by many virus- and putatively some cell-encoded templates. Internal initiation is facilitated by specific RNA tertiary folds, called internal ribosomal entry sites (IRESs), in the 5' untranslated region (UTR) of the respective transcripts. In this review we discuss recent structural insight into how established IRESs first capture and then manipulate the eukaryotic translation machinery through non-canonical interactions and by guiding the intrinsic conformational flexibility of the eukaryotic ribosome...
July 3, 2017: Trends in Biochemical Sciences
https://www.readbyqxmd.com/read/28683393/flavivirus-structural-heterogeneity-implications-for-cell-entry
#19
REVIEW
Félix A Rey, Karin Stiasny, Franz X Heinz
The explosive spread of Zika virus is the most recent example of the threat imposed to human health by flaviviruses. High-resolution structures are available for several of these arthropod-borne viruses, revealing alternative icosahedral organizations of immature and mature virions. Incomplete proteolytic maturation, however, results in a cloud of highly heterogeneous mosaic particles. This heterogeneity is further expanded by a dynamic behavior of the viral envelope glycoproteins. The ensemble of heterogeneous and dynamic infectious particles circulating in infected hosts offers a range of alternative possible receptor interaction sites at their surfaces, potentially contributing to the broad flavivirus host-range and variation in tissue tropism...
July 3, 2017: Current Opinion in Virology
https://www.readbyqxmd.com/read/28683318/analysis-of-iav-replication-and-co-infection-dynamics-by-a-versatile-rna-viral-genome-labeling-method
#20
Dan Dou, Iván Hernández-Neuta, Hao Wang, Henrik Östbye, Xiaoyan Qian, Swantje Thiele, Patricia Resa-Infante, Nancy Mounogou Kouassi, Vicky Sender, Karina Hentrich, Peter Mellroth, Birgitta Henriques-Normark, Gülsah Gabriel, Mats Nilsson, Robert Daniels
Genome delivery to the proper cellular compartment for transcription and replication is a primary goal of viruses. However, methods for analyzing viral genome localization and differentiating genomes with high identity are lacking, making it difficult to investigate entry-related processes and co-examine heterogeneous RNA viral populations. Here, we present an RNA labeling approach for single-cell analysis of RNA viral replication and co-infection dynamics in situ, which uses the versatility of padlock probes...
July 5, 2017: Cell Reports
keyword
keyword
56323
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"