Gaia Pucci, Luigi Minafra, Valentina Bravatà, Marco Calvaruso, Giuseppina Turturici, Francesco P Cammarata, Gaetano Savoca, Boris Abbate, Giorgio Russo, Vincenzo Cavalieri, Giusi I Forte
The hypoxic pattern of glioblastoma (GBM) is known to be a primary cause of radioresistance. Our study explored the possibility of using gene knockdown of key factors involved in the molecular response to hypoxia, to overcome GBM radioresistance. We used the U87 cell line subjected to chemical hypoxia generated by CoCl2 and exposed to 2 Gy of X-rays, as single or combined treatments, and evaluated gene expression changes of biomarkers involved in the Warburg effect, cell cycle control, and survival to identify the best molecular targets to be knocked-down, among those directly activated by the HIF-1α transcription factor...
February 8, 2024: International Journal of Molecular Sciences