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https://www.readbyqxmd.com/read/29277765/traditional-chinese-medicine-curcumin-sensitizes-human-colon-cancer-to-radiation-by-altering-the-expression-of-dna-repair-related-genes
#1
Guangen Yang, Jianming Qiu, Dong Wang, Yong Tao, Yihuan Song, Hongtao Wang, Juping Tang, Xing Wang, Y U Sun, Zhijian Yang, Robert M Hoffman
BACKGROUND/AIM: The aim of the present study was to investigate the radio-sensitizing efficacy of curcumin, a traditional Chinese medicine (TCM) on colon cancer cells in vitro and in vivo. MATERIALS AND METHODS: Human colon cancer HT-29 cells were treated with curcumin (2.5 μM), irradiation (10 Gy) and the combination of irradiation and curcumin. Cell proliferation was assessed using the MTT assay. Apoptotic cells were detected by Annexin V-PE/7-AAD analysis. PCR was performed to determine differential-expression profiling of 95 DNA-repair genes in irradiated cells and cells treated with both irradiation and curcumin...
January 2018: Anticancer Research
https://www.readbyqxmd.com/read/29049411/xrcc5-cooperates-with-p300-to-promote-cyclooxygenase-2-expression-and-tumor-growth-in-colon-cancers
#2
Zhifeng Zhang, Fufu Zheng, Zhenlong Yu, Jiajiao Hao, Miao Chen, Wendan Yu, Wei Guo, Yiming Chen, Wenlin Huang, Zhijun Duan, Wuguo Deng
Cyclooxygenase (COX) is the rate-limiting enzyme in prostaglandins (PGs) biosynthesis. Previous studies indicate that COX-2, one of the isoforms of COX, is highly expressed in colon cancers and plays a key role in colon cancer carcinogenesis. Thus, searching for novel transcription factors regulating COX-2 expression will facilitate drug development for colon cancer. In this study, we identified XRCC5 as a binding protein of the COX-2 gene promoter in colon cancer cells with streptavidin-agarose pulldown assay and mass spectrometry analysis, and found that XRCC5 promoted colon cancer growth through modulation of COX-2 signaling...
2017: PloS One
https://www.readbyqxmd.com/read/28976774/proteomic-analysis-of-extracellular-hmgb1-identifies-binding-partners-and-exposes-its-potential-role-in-airway-epithelial-cell-homeostasis
#3
Sharon L Wong, Joyce To, Jerran Santos, Venkata Sita Rama Raju Allam, John P Dalton, Steven P Djordjevic, Sheila Donnelly, Matthew P Padula, Maria B Sukkar
The release of damage-associated molecular patterns (DAMPs) by airway epithelial cells is believed to play a crucial role in the initiation and development of chronic airway conditions such as asthma and chronic obstructive pulmonary disease (COPD). Intriguingly, the classic DAMP high-mobility group box-1 (HMGB1) is detected in the culture supernatant of airway epithelial cells under basal conditions, indicating a role for HMGB1 in the regulation of epithelial cellular and immune homeostasis. To gain contextual insight into the potential role of HMGB1 in airway epithelial cell homeostasis, we used the orthogonal and complementary methods of high-resolution clear native electrophoresis, immunoprecipitation, and pull-downs coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) to profile HMGB1 and its binding partners in the culture supernatant of unstimulated airway epithelial cells...
November 14, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28927264/comparative-interactomes-of-vrk1-and-vrk3-with-their-distinct-roles-in-the-cell-cycle-of-liver-cancer
#4
Namgyu Lee, Dae-Kyum Kim, Seung Hyun Han, Hye Guk Ryu, Sung Jin Park, Kyong-Tai Kim, Kwan Yong Choi
Vaccinia-related kinase 1 (VRK1) and VRK3 are members of the VRK family of serine/threonine kinases and are principally localized in the nucleus. Despite the crucial roles of VRK1/VRK3 in physiology and disease, the molecular and functional interactions of VRK1/VRK3 are poorly understood. Here, we identified over 200 unreported VRK1/VRK3-interacting candidate proteins by affinity purification and LC-MS/MS. The networks of VRK1 and VRK3 interactomes were found to be associated with important biological processes such as the cell cycle, DNA repair, chromatin assembly, and RNA processing...
September 30, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28536799/stress-induced-transcriptional-changes-and-dna-damage-associated-with-bis-2-ethylhexyl-adipate-exposure-in-zebrafish-danio-rerio-larvae
#5
Halis Boran, Serap Terzi
The present study evaluates potential toxic effects of bis(2-ethylhexyl) adipate (DEHA) plasticizer to larval (72 h post fertilization) zebrafish (Danio rerio) by analyzing changes in expression levels of stress-related genes (p53, rad51 and xrcc5) and assessing possible DNA damage of DEHA in larvae. The lethal concentration for 50% mortality (LC50) in larval zebrafish exposed for 96 h to 0-200 mg L(-1) DEHA was 89.9 ± 8.03 mg L(-1). A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed for 96 h to DEHA...
September 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28421111/xrcc5-vntr-xrcc6-61c-g-and-xrcc7-6721g-t-gene-polymorphisms-associated-with-male-infertility-risk-evidences-from-case-control-and-in-silico-studies
#6
Danial Jahantigh, Abasalt Hosseinzadeh Colagar
We evaluate the association between genetic polymorphisms of XRCC5 VNTR, XRCC6 -61C>G, and XRCC7 6721G>T with male infertility susceptibility. A total of 392 men including 178 infertile males (102 idiopathic azoospermia and 76 severe oligozoospermia) and 214 healthy controls were recruited. XRCC6 -61C>G and XRCC7 6721G>T genotyping was performed by PCR-RFLP whereas XRCC5 VNTR was performed by PCR. The 2R allele and 2R allele carriers of XRCC5 VNTR polymorphism significantly decreased risk of male infertility...
2017: International Journal of Endocrinology
https://www.readbyqxmd.com/read/28368303/identification-of-hiv-1-tat-associated-proteins-contributing-to-hiv-1-transcription-and-latency
#7
Maxime Junior Jean, Derek Power, Weili Kong, Huachao Huang, Netty Santoso, Jian Zhu
Human immunodeficiency virus type 1 (HIV-1) Tat is a virus-encoded trans-activator that plays a central role in viral transcription. We used our recently developed parallel analysis of in vitro translated open reading frames (ORFs) (PLATO) approach to identify host proteins that associate with HIV-1 Tat. From this proteomic assay, we identify 89 Tat-associated proteins (TAPs). We combine our results with other datasets of Tat or long terminal repeat (LTR)-associated proteins. For some of these proteins (NAT10, TINP1, XRCC5, SIN3A), we confirm their strong association with Tat...
April 1, 2017: Viruses
https://www.readbyqxmd.com/read/28107606/dishevelled-segment-polarity-protein-3-dvl3-a-novel-and-easily-applicable-recurrence-predictor-in-localised-prostate-adenocarcinoma
#8
Pil-Jong Kim, Ji Y Park, Hong-Gee Kim, Yong Mee Cho, Heounjeong Go
OBJECTIVE: To identify new biomarkers for biochemical recurrence (BCR) of prostate adenocarcinoma. PATIENTS AND METHODS: Clinical information of 500 patients with prostate adenocarcinoma and their 152 RNA-sequencing and protein-array data from The Cancer Genome Atlas (TCGA) were separated into a discovery set and a validation set. Each dataset was analysed according to the Gleason grade groups reflecting BCR. The results obtained from the analysis using TCGA dataset were confirmed by immunohistochemistry analyses of a confirmation cohort composed of 395 patients with localised prostate adenocarcinoma...
September 2017: BJU International
https://www.readbyqxmd.com/read/28035050/chromatin-association-of-xrcc5-6-in-the-absence-of-dna-damage-depends-on-the-xpe-gene-product-ddb2
#9
Damiano Fantini, Shuo Huang, John M Asara, Srilata Bagchi, Pradip Raychaudhuri
Damaged DNA-binding protein 2 (DDB2), a nuclear protein, participates in both nucleotide excision repair and mRNA transcription. The transcriptional regulatory function of DDB2 is significant in colon cancer, as it regulates metastasis. To characterize the mechanism by which DDB2 participates in transcription, we investigated the protein partners in colon cancer cells. Here we show that DDB2 abundantly associates with XRCC5/6, not involving CUL4 and DNA-PKcs. A DNA-damaging agent that induces DNA double-stranded breaks (DSBs) does not affect the interaction between DDB2 and XRCC5...
January 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28025995/prediction-of-key-genes-and-mirnas-responsible-for-loss-of-muscle-force-in-patients-during-an-acute-exacerbation-of-chronic-obstructive-pulmonary-disease
#10
Yanhong Duan, Min Zhou, Jian Xiao, Chaomin Wu, Lei Zhou, Feng Zhou, Chunling Du, Yuanlin Song
The present study aimed to identify genes and microRNAs (miRNAs or miRs) that were abnormally expressed in the vastus lateralis muscle of patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD). The gene expression profile of GSE10828 was downloaded from the Gene Expression Omnibus database, and this dataset was comprised of 4 samples from patients with AECOPD and 5 samples from patients with stable COPD. Differentially expressed genes (DEGs) were screened using the Limma package in R...
November 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28008145/msh2-brca1-expression-as-a-dna-repair-signature-predicting-survival-in-early-stage-lung-cancer-patients-from-the-ifct-0002-phase-3-trial
#11
Guénaëlle Levallet, Fatéméh Dubois, Pierre Fouret, Martine Antoine, Solenn Brosseau, Emmanuel Bergot, Michèle Beau-Faller, Valérie Gounant, Elisabeth Brambilla, Didier Debieuvre, Olivier Molinier, Françoise Galateau-Sallé, Julien Mazieres, Elisabeth Quoix, Jean-Louis Pujol, Denis Moro-Sibilot, Alexandra Langlais, Franck Morin, Virginie Westeel, Gérard Zalcman
INTRODUCTION: DNA repair is a double-edged sword in lung carcinogenesis. When defective, it promotes genetic instability and accumulated genetic alterations. Conversely these defects could sensitize cancer cells to therapeutic agents inducing DNA breaks. METHODS: We used immunohistochemistry (IHC) to assess MSH2, XRCC5, and BRCA1 expression in 443 post-chemotherapy specimens from patients randomized in a Phase 3 trial, comparing two neoadjuvant regimens in 528 Stage I-II non-small cell lung cancer (NSCLC) patients (IFCT-0002)...
January 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/27852033/the-relationship-between-polymorphisms-of-xrcc5-genes-with-astrocytoma-prognosis-in-the-han-chinese-population
#12
Xue He, Xikai Zhu, Lei Li, Jiayi Zhang, Ruipeng Wu, Yuan Zhang, Longli Kang, Dongya Yuan, Tianbo Jin
BACKGROUND: Gliomas are highly malignant with a poor prognosis. Studies have reported that DNA repair genes influence risk for glioma, but its relationship with prognosis is unclear. In this study, we want to explore the relationship between DNA repair genes (XRCC3, XRCC4 and XRCC5) and prognosis of astrocytoma in the Chinese Han population. MATERIALS AND METHODS: 160 astrocytoma cases were recruited in our study. Survival probabilities were estimated by using Kaplan-Meier analysis, and significant differences were analyzed by using the log-rank test...
December 20, 2016: Oncotarget
https://www.readbyqxmd.com/read/27626685/pathological-complete-response-after-cisplatin-neoadjuvant-therapy-is-associated-with-the-downregulation-of-dna-repair-genes-in-brca1-associated-triple-negative-breast-cancers
#13
Pawel Domagala, Jolanta Hybiak, Janusz Rys, Tomasz Byrski, Cezary Cybulski, Jan Lubinski
Pathologic complete response (pCR) after neoadjuvant chemotherapy is considered a suitable surrogate marker of treatment efficacy in patients with triple-negative breast cancers (TNBCs). However, the molecular mechanisms underlying pCR as a result of such treatment remain obscure. Using real-time PCR arrays we compared the expression levels of 120 genes involved in the main mechanisms of DNA repair in 43 pretreatment biopsies of BRCA1-associated TNBCs exhibiting pCR and no pathological complete response (non-pCR) after neoadjuvant chemotherapy with cisplatin...
October 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27497341/influence-of-functional-polymorphisms-in-dna-repair-genes-of-myelodysplastic-syndrome
#14
Howard Lopes Ribeiro, Allan Rodrigo Soares Maia, Marília Braga Costa, Izabelle Rocha Farias, Daniela de Paula Borges, Roberta Taiane Germano de Oliveira, Juliana Cordeiro de Sousa, Silvia Maria Meira Magalhães, Ronald Feitosa Pinheiro
Myelodysplastic syndromes (MDS) are a heterogeneous group of hematopoietic stem cell (HSC) malignances characterized by peripheral cytopenias and predisposition to acute myeloid leukemia transformation. Several studies show that the MDS pathogenesis is a complex and heterogeneous process that involves multiple steps through a sequence of genetic lesions in the DNA which lead to functional changes in the cell and the emergence and subsequent evolution of pre-malignant clone. Double strand breaks (DSB) lesions are the most severe type of DNA damage in HSCs, which, if not properly repaired, might contribute to the development of chromosomal abnormalities, which in turn may lead to leukemia development...
September 2016: Leukemia Research
https://www.readbyqxmd.com/read/27249627/human-annulus-progenitor-cells-analyses-of-this-viable-endogenous-cell-population
#15
Helen E Gruber, Frank E Riley, Gretchen L Hoelscher, Jane A Ingram, Letitia Bullock, Edward N Hanley
Back pain and intervertebral disc degeneration have growing socioeconomic/health care impacts. Increasing research efforts address use of stem and progenitor cell-based replacement therapies to repopulate and regenerate the disc. Data presented here on the innate human annulus progenitor cells: (i) assessed osteogenic, chondrogenic and adipogenic potentials of cultured human annulus cells; and (ii) defined progenitor-cell related gene expression patterns. Verification of the presence of progenitor cells within primary human disc tissue also used immunohistochemical identification of cell surface markers and microarray analyses...
August 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27248496/wdr76-co-localizes-with-heterochromatin-related-proteins-and-rapidly-responds-to-dna-damage
#16
Joshua M Gilmore, Mihaela E Sardiu, Brad D Groppe, Janet L Thornton, Xingyu Liu, Gerald Dayebgadoh, Charles A Banks, Brian D Slaughter, Jay R Unruh, Jerry L Workman, Laurence Florens, Michael P Washburn
Proteins that respond to DNA damage play critical roles in normal and diseased states in human biology. Studies have suggested that the S. cerevisiae protein CMR1/YDL156w is associated with histones and is possibly associated with DNA repair and replication processes. Through a quantitative proteomic analysis of affinity purifications here we show that the human homologue of this protein, WDR76, shares multiple protein associations with the histones H2A, H2B, and H4. Furthermore, our quantitative proteomic analysis of WDR76 associated proteins demonstrated links to proteins in the DNA damage response like PARP1 and XRCC5 and heterochromatin related proteins like CBX1, CBX3, and CBX5...
2016: PloS One
https://www.readbyqxmd.com/read/27148484/differences-of-variable-number-tandem-repeats-in-xrcc5-promoter-are-associated-with-increased-or-decreased-risk-of-breast-cancer-in-brca-gene-mutation-carriers
#17
Jian Cui, Jiangtao Luo, Yeong C Kim, Carrie Snyder, Dina Becirovic, Bradley Downs, Henry Lynch, San Ming Wang
Ku80 is a subunit of the Ku heterodimer that binds to DNA double-strand break ends as part of the non-homologous end joining (NHEJ) pathway. Ku80 is also involved in homologous recombination (HR) via its interaction with BRCA1. Ku80 is encoded by the XRCC5 gene that contains a variable number tandem repeat (VNTR) insertion in its promoter region. Different VNTR genotypes can alter XRCC5 expression and affect Ku80 production, thereby affecting NHEJ and HR pathways. VNTR polymorphism is associated with multiple types of sporadic cancer...
2016: Frontiers in Oncology
https://www.readbyqxmd.com/read/26974709/polymorphisms-in-double-strand-break-repair-related-genes-influence-radiosensitivity-phenotype-in-lymphocytes-from-healthy-individuals
#18
Kamalesh Dattaram Mumbrekar, Hassan Venkatesh Goutham, Bejadi Manjunath Vadhiraja, Satish Rao Bola Sadashiva
BACKGROUND: A range of individual radiosensitivity observed in humans can influence individual's susceptibility toward cancer risk and radiotherapy outcome. Therefore, it is important to measure the variation in radiosensitivity and to identify the genetic factors influencing it. METHODS: By adopting a pathway specific genotype-phenotype design, we established the variability in cellular radiosensitivity by performing γ-H2AX foci assay in healthy individuals. Further, we genotyped ten selected SNPs in candidate genes XRCC3 (rs861539), XRCC4 (rs1805377), XRCC5 (rs3835), XRCC6 (rs2267437), ATM (rs3218698, rs1800057), LIG4 (rs1805388), NBN (rs1805794), RAD51 (rs1801320) and PRKDC (rs7003908), and analysed their influence on observed variation in radiosensitivity...
April 2016: DNA Repair
https://www.readbyqxmd.com/read/26918347/environmental-exposure-and-hpv-infection-may-act-synergistically-to-induce-lung-tumorigenesis-in-nonsmokers
#19
Ya-Wen Cheng, Frank Cheau-Feng Lin, Chih-Yi Chen, Nan-Yung Hsu
Most studies of lung tumorigenesis have focused on smokers rather than nonsmokers. In this study, we used human papillomavirus (HPV)-positive and HPV-negative lung cancer cells to test the hypothesis that HPV infection synergistically increases DNA damage induced by exposure to the carcinogen benzo[a]pyrene (B[a]P), and contributes to lung tumorigenesis in nonsmokers. DNA adduct levels induced by B[a]P in HPV-positive cells were significantly higher than in HPV-negative cells. The DNA adduct formation was dependent on HPV E6 oncoprotein expression...
April 12, 2016: Oncotarget
https://www.readbyqxmd.com/read/26870890/genetic-and-epigenetic-background-and-protein-expression-profiles-in-relation-to-telomerase-activation-in-medullary-thyroid-carcinoma
#20
Na Wang, Hanna Kjellin, Anastasios Sofiadis, Omid Fotouhi, C Christofer Juhlin, Martin Bäckdahl, Jan Zedenius, Dawei Xu, Janne Lehtiö, Catharina Larsson
Medullary thyroid carcinomas (MTCs) exhibit telomerase activation in strong association with shorter patient survival. To understand the background of telomerase activation we quantified TERT copy numbers and TERT promoter methylation in 42 MTCs and normal thyroid references. Gain of TERT was demonstrated by quantitative PCR in 5/39 sporadic MTC. Increased methylation index (MetI) for CpG methylation at the TERT promoter was found in sporadic MTCs (P < 0.0001) and in MEN 2 associated MTCs (P = 0.011) vs...
April 19, 2016: Oncotarget
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