keyword
MENU ▼
Read by QxMD icon Read
search

PI3

keyword
https://www.readbyqxmd.com/read/28100485/quantification-of-growth-factor-signaling-and-pathway-crosstalk-by-live-cell-imaging
#1
Sean M Gross, Peter Rotwein
Peptide growth factors stimulate cellular responses through activation of their trans-membrane receptors. Multiple intracellular signaling cascades are engaged following growth factor - receptor binding, leading to short- and long-term biological effects. Each receptor-activated signaling pathway does not act in isolation, but rather interacts at different levels with other pathways to shape signaling networks that are distinctive for each growth factor. To gain insights into the specifics of growth factor-regulated interactions among different signaling cascades, we developed a HeLa cell line stably expressing fluorescent live-cell imaging reporters that are readouts for two major growth factor-stimulated pathways, Ras - Raf - Mek - Erk and PI3-kinase - Akt...
January 18, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28095750/effects-of-pre-germinated-brown-rice-treatment-high-fat-diet-induced-metabolic-syndrome-in-c57bl-6j-mice
#2
Hsueh-Wei Yen, Hui-Li Lin, Chi-Long Hao, Fu-Chih Chen, Chun-Yun Chen, Jia-Hao Chen, Kuo-Ping Shen
To investigate using pre-germinated brown rice (PGBR) to treat metabolic syndrome, we fed one group of mice standard-regular-diet (SRD) for 20 weeks and another group of mice high-fat-diet (HFD) for 16 weeks. We subdivided them into HFD group and HFD + PGBR group whose dietary carbohydrate was replaced with PGBR for 4 weeks. The HFD group gained more weight, had higher blood pressure, heart rate, blood glucose and lipids, liver levels of TG, feces TG and bile acid, lower adipose levels of adipocytokine, lower skeletal muscle IR, IRS-1, IRS-2, PI3 K, Akt/PKB, GLUT-1, GLUT-4, GCK and PPAR-γ; higher liver SREBP-1, SCD-1, FAS, HMGCR, LDLR, CYP7α1 and PPAR-α, and higher adipose SREBP-1, SCD-1, FAS, and lower adipose PPAR-α and adiponectin...
January 18, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28087538/bcap-inhibits-proliferation-and-differentiation-of-myeloid-progenitors-in-the-steady-state-and-during-demand-situations
#3
Jeffrey M Duggan, Matthew B Buechler, Rebecca M Olson, Tobias M Hohl, Jessica A Hamerman
B cell adaptor for PI3-kinase (BCAP) is a signaling adaptor expressed in mature hematopoietic cells including monocytes and neutrophils. Here we investigated the role of BCAP in the homeostasis and development of these myeloid lineages. BCAP(-/-) mice had more bone marrow (BM) monocytes than WT mice, and in mixed WT:BCAP(-/-) BM chimeras, monocytes and neutrophils skewed towards BCAP(-/-) origin, showing a competitive advantage for BCAP(-/-) myeloid cells. BCAP was expressed in bone marrow hematopoietic progenitors, including LSK (Lineage(-)Sca1(+)cKit(+)), CMP (Common Myeloid Progenitor) and GMP (Granulocyte/Macrophage Progenitor) cells...
January 13, 2017: Blood
https://www.readbyqxmd.com/read/28080070/solution-synthesis-of-iodine-doped-red-phosphorus-nanoparticles-for-lithium-ion-battery-anodes
#4
Wei-Chung Chang, Kuan-Wei Tseng, Hsing-Yu Tuan
Red Phosphorus (RP) is a promising anode material for lithium-ion battery due to its earth abundance and a high theoretical capacity of 2596 mA h g-1. Although RP-based anodes for lithium-ion batteries have been reported, they were all in the form of carbon/P composites, including P-graphene, P-graphite, P-CNTs and P-carbon black, in order to improve P's extremely low conductivity and large volume change during cycling process. Here we report the large-scale synthesis of red phosphorus nanoparticles (RPNPs) with sizes ranging from 100 to 200 nm by reacting PI3 with ethylene glycol in the presence of cetyltrimethylammonium bromide (CTAB) in ambient environment...
January 12, 2017: Nano Letters
https://www.readbyqxmd.com/read/28073120/nano-pelargonidin-protects-hyperglycemic-induced-l6-cells-against-mitochondrial-dysfunction
#5
Asmita Samadder, Debojyoti Tarafdar, Suresh K Abraham, Kumaresh Ghosh, Anisur Rahman Khuda-Bukhsh
Nano-encapsulation of several natural products has become an important tool in enhancing the bioavailability of some modern drugs against many diseases. Pelargonidin is an anthocyanidin found in many fruits and vegetables. Pelargonidin is loaded with poly-lactide-co-glycolic-acid, a non-toxic biodegradable polymer, to produce nano-pelargonidin. Size, morphology, zeta potential, and planar uniformity of formulated nano-pelargonidin were determined by atomic force microscopy and dynamic light scattering. The time required for cellular entry, folds of nano-pelargonidin, and drug encapsulation efficiency of poly-lactide-co-glycolic-acid were also ascertained...
January 10, 2017: Planta Medica
https://www.readbyqxmd.com/read/28072855/sdf-1%C3%AE-cxcr4-signaling-in-lipid-rafts-induces-platelet-aggregation-via-pi3-kinase-dependent-akt-phosphorylation
#6
Hiroko Ohtsuka, Tomohiro Iguchi, Moyuru Hayashi, Mizuho Kaneda, Kazuko Iida, Motoyuki Shimonaka, Takahiko Hara, Morio Arai, Yuichi Koike, Naomasa Yamamoto, Kohji Kasahara
Stromal cell-derived factor-1α (SDF-1α)-induced platelet aggregation is mediated through its G protein-coupled receptor CXCR4 and phosphatidylinositol 3 kinase (PI3K). Here, we demonstrate that SDF-1α induces phosphorylation of Akt at Thr308 and Ser473 in human platelets. SDF-1α-induced platelet aggregation and Akt phosphorylation are inhibited by pretreatment with the CXCR4 antagonist AMD3100 or the PI3K inhibitor LY294002. SDF-1α also induces the phosphorylation of PDK1 at Ser241 (an upstream activator of Akt), GSK3β at Ser9 (a downstream substrate of Akt), and myosin light chain at Ser19 (a downstream element of the Akt signaling pathway)...
2017: PloS One
https://www.readbyqxmd.com/read/28063948/cytokine-il-10-activators-of-pi3-kinase-agonists-of-%C3%AE-2-adrenoreceptor-and-antioxidants-prevent-ischemia-induced-cell-death-in-rat-hippocampal-cultures
#7
Egor A Turovsky, Maria V Turovskaya, Sergei G Gaidin, Valery P Zinchenko
In the present work we compared the protective effect of anti-inflammatory cytokine IL-10 with the action of a PI3-kinase selective activator 740 Y-P, selective agonists of alpha-2 adrenoreceptor, guanfacine and UK-14,304, and compounds having antioxidant effect: recombinant human peroxiredoxin 6 and B27, in hippocampal cell culture during OGD (ischemia-like conditions). It has been shown that the response of cells to OGD in the control includes two phases. The first phase was accompanied by an increase in the frequency of spontaneous synchronous Ca(2+)-oscillations (SSCO) in neurons and Ca(2+)-pulse in astrocytes...
January 4, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28054203/molecular-explorations-of-substituted-2-4-phenylquinolin-2-yl-phenols-as-phosphoinositide-3-kinase-inhibitors-and-anticancer-agents
#8
Manikandan Alagumuthu, Sivakumar Arumugam
PURPOSE: Substituted 2-(4-phenylquinolin-2-yl) phenols (PQPDs) emerged as the inhibitors of phosphoinositide 3-kinase (PI3K) and anticancer agents. METHOD: PI3K inhibition was assessed by competitive ELISA. Anticancer activity was evaluated against breast cancer (MCF-7), skin cancer (G-361), and colon cancer (HCT 116) cell lines. RESULTS: In PI3 Kinase assay, PQPDs 4c, 4d, and 4k were inactive with IC50 >5 µM. IC50 for 4a, 4b, 4f-h, and 4j was ≥0...
January 4, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28050799/thyroid-hormone-controls-breast-cancer-cell-movement-via-integrin-%C3%AE-v-%C3%AE-3-src-fak-pi3-kinases
#9
Marina Inés Flamini, Ivonne Denise Uzair, Gisela Erika Pennacchio, Flavia Judith Neira, Joselina Magali Mondaca, Fernando Dario Cuello-Carrión, Graciela Alma Jahn, Tommaso Simoncini, Angel Matías Sanchez
Thyroid hormones (TH) play a fundamental role in diverse processes, including cellular movement. Cell migration requires the integration of events that induce changes in cell structure towards the direction of migration. These actions are driven by actin remodeling and stabilized by the development of adhesion sites to extracellular matrix via transmembrane receptors linked to the actin cytoskeleton. Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that promotes cell migration and invasion through the control of focal adhesion turnover...
January 3, 2017: Hormones & Cancer
https://www.readbyqxmd.com/read/28042706/do-cdk4-6-inhibitors-have-potential-as-targeted-therapeutics-for-squamous-cell-cancers
#10
Nene N Kalu, Faye M Johnson
Introduction Dysregulation of cell cycle progression has an established link to neoplasia and cancer progression. Components of the cyclin D-CDK4/6-INK4-Rb pathway are frequently altered in squamous cell carcinomas (SCCs) by diverse mechanisms, including viral oncogene-induced degradation, mutation, deletion, and amplification. Activation of the CDK4/6 pathway may predict response to CDK4/6 inhibitors and provide clinical biomarkers. Recently, the CDK4/6 inhibitor palbociclib showed clinical efficacy in combination with cetuximab in HNSCC patients...
January 1, 2017: Expert Opinion on Investigational Drugs
https://www.readbyqxmd.com/read/28034890/a-novel-mechanism-regulating-human-platelet-activation-by-mmp-2-mediated-par1-biased-signaling
#11
Manuela Sebastiano, Stefania Momi, Emanuela Falcinelli, Loredana Bury, Marc Hoylaerts, Paolo Gresele
Platelets contain and release several matrix metalloproteinases (MMPs). Among these, active MMP-2 enhances platelet aggregation by favouring the activation of phosphatidyl-inositol-3- kinase (PI3-K) and contributes to arterial thrombosis. The platelet surface target of MMP-2 and the mechanism through which it primes platelets to respond to subsequent stimuli are still unknown. We show that active MMP-2 enhances platelet activation induced by weak stimuli by cleaving PAR1 at a non-canonical extracellular site different from the thrombin-cleavage site, and thus starts biased receptor signaling triggering only some of the signaling pathways normally activated by full PAR1 agonism...
December 29, 2016: Blood
https://www.readbyqxmd.com/read/28017896/augmented-o-glcnac-signaling-via-glucosamine-attenuates-oxidative-stress-and-apoptosis-following-contrast-induced-acute-kidney-injury-in-rats
#12
Jiachang Hu, Rongyi Chen, Ping Jia, Yi Fang, Tongqiang Liu, Nana Song, Xialian Xu, Jun Ji, Xiaoqiang Ding
Contrast-induced acute kidney injury (CI-AKI) is an iatrogenic renal injury and associated with substantial morbidity and mortality in susceptible individuals. Despite extensive study of a variety of agents for renal protection, limited strategies have been shown to be effective in the reduction of CI-AKI. O-linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational regulatory modification of intracellular proteins and governs the function of numerous proteins, both cytosolic and nuclear. Increasing evidence suggests that O-GlcNAc levels are increased in response to stress and that acute augmentation of this reaction is cytoprotective...
December 23, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28011152/adenosine-a2a-receptor-stimulation-blocks-development-of-nonalcoholic-steatohepatitis-in-mice-by-multilevel-inhibition-of-signals-that-cause-immunolipotoxicity
#13
Elisa Alchera, Simona Rolla, Chiara Imarisio, Valentina Bardina, Guido Valente, Francesco Novelli, Rita Carini
Lipotoxicity and immunoinflammation are associated with the evolution of steatosis toward nonalcoholic steatohepatitis (NASH). This study reports the ability of adenosine A2a receptor (A2aR) activation to inhibit NASH development by modulating the responses of CD4(+) T-helper (Th) cells to avoid an immuno-mediated potentiation of lipotoxicity. The effect of the A2aR agonist CGS21680 on immunoinflammatory signals, CD4(+)Th cell infiltration and immunolipotoxicity was analyzed in steatotic C57BL/6 mice fed with a methionine-choline-deficient (MCD) diet and in mouse hepatocytes exposed to palmitic acid (PA)...
December 6, 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28009298/adenovirus-protein-e4-orf1-activation-of-pi3-kinase-reveals-differential-regulation-of-downstream-effector-pathways-in-adipocytes
#14
Natasha Chaudhary, Eva Gonzalez, Sung-Hee Chang, Fuqiang Geng, Shahin Rafii, Nasser K Altorki, Timothy E McGraw
Insulin activation of phosphatidylinositol 3-kinase (PI3K) regulates metabolism, including the translocation of the Glut4 glucose transporter to the plasma membrane and inactivation of the FoxO1 transcription factor. Adenoviral protein E4-ORF1 stimulates cellular glucose metabolism by mimicking growth-factor activation of PI3K. We have used E4-ORF1 as a tool to dissect PI3K-mediated signaling in adipocytes. E4-ORF1 activation of PI3K in adipocytes recapitulates insulin regulation of FoxO1 but not regulation of Glut4...
December 20, 2016: Cell Reports
https://www.readbyqxmd.com/read/28008177/mass-cytometry-analysis-reveals-hyperactive-nf-kappa-b-signaling-in-myelofibrosis-and-secondary-acute-myeloid-leukemia
#15
D A C Fisher, O Malkova, E K Engle, C Miner, M C Fulbright, G K Behbehani, T B Collins, S Bandyopadhyay, A Zhou, G P Nolan, S T Oh
Myeloproliferative neoplasms (MPNs) feature a malignant clone containing the JAK2 V617F mutation, or another mutation causing dysregulated JAK2 kinase activity. The multiple disease phenotypes of MPNs, and their tendency to transform phenotypically, suggest pathophysiologic heterogeneities beyond a common phenomenon of JAK2 hyperactivation. JAK2 has the potential to activate multiple other signaling molecules, either directly through downstream effectors, or indirectly through induction of target gene expression...
December 23, 2016: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/28000962/hypoxia-causes-il-8-secretion-charcot-leyden-crystal-formation-and-suppression-of-corticosteroid-induced-apoptosis-in-human-eosinophils
#16
Linsey M Porter, Andrew S Cowburn, Neda Farahi, John Deighton, Stuart N Farrow, Christine A Fiddler, Jatinder K Juss, Alison M Condliffe, Edwin R Chilvers
BACKGROUND: Inflamed environments are typically hypercellular, rich in pro-inflammatory cytokines, and profoundly hypoxic. While the effects of hypoxia on neutrophil longevity and function have been widely studied, little is known about the consequences of this stimulus on eosinophils. OBJECTIVE: We sought to investigate the effects of hypoxia on several key aspects of eosinophil biology; namely secretion, survival, and their sensitivity to glucocorticosteroids (GCS), agents which normally induce eosinophil apoptosis...
December 21, 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27997620/molecular-and-serological-survey-of-selected-viruses-in-free-ranging-wild-ruminants-in-iran
#17
Farhid Hemmatzadeh, Wayne Boardman, Arezo Alinejad, Azar Hematzade, Majid Kharazian Moghadam
A molecular and serological survey of selected viruses in free-ranging wild ruminants was conducted in 13 different districts in Iran. Samples were collected from 64 small wild ruminants belonging to four different species including 25 Mouflon (Ovis orientalis), 22 wild goat (Capra aegagrus), nine Indian gazelle (Gazella bennettii) and eight Goitered gazelle (Gazella subgutturosa) during the national survey for wildlife diseases in Iran. Serum samples were evaluated using serologic antibody tests for Peste de petits ruminants virus (PPRV), Pestiviruses [Border Disease virus (BVD) and Bovine Viral Diarrhoea virus (BVDV)], Bluetongue virus (BTV), Bovine herpesvirus type 1 (BHV-1), and Parainfluenza type 3 (PI3)...
2016: PloS One
https://www.readbyqxmd.com/read/27989877/chinese-herbal-medicine-fuzheng-kang-ai-decoction-sensitized-the-effect-of-gefitinib-on-inhibition-of-human-lung-cancer-cells-through-inactivating-pi3-k-akt-mediated-suppressing-muc1-expression
#18
Longmei Li, SuMei Wang, Fang Zheng, WanYin Wu, Swei Sunny Hann
ETHNOPHARMACOLOGICAL RELEVANCE: Chinese herbal medicine (CHM) Fuzheng Kang-Ai (FZKA for short) decoction has been used as adjuvant treatment strategies in lung cancer patients for decades. However, the molecular mechanism underlying the therapeutic potential especially in sensitizing the effect of EGFR-TKI gefitinib has not been well elucidated. MATERIALS AND METHODS: Cell viability was detected by MTT assay. Cell cycle distribution was detected by flow cytometry...
October 28, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27981377/myrrh-exerts-barrier-stabilising-and-protective-effects-in-ht-29-b6-and-caco-2-intestinal-epithelial-cells
#19
Rita Rosenthal, Julia Luettig, Nina A Hering, Susanne M Krug, Uwe Albrecht, Michael Fromm, Jörg-Dieter Schulzke
PURPOSE: Myrrh, the oleo-gum resin of Commiphora molmol, is well known for its anti-inflammatory properties. In different animal models, it protected against DSS-, TNBS- and oxazolone-induced colitis. To date, no information concerning the effect of myrrh on barrier properties are available. Thus, this study investigates the effect of myrrh on paracellular barrier function in the absence or presence of the pro-inflammatory cytokine TNFα. METHODS: Monolayers of human colon cell lines HT-29/B6 and Caco-2 were incubated with myrrh under control conditions or after challenge with the pro-inflammatory cytokine TNFα...
December 15, 2016: International Journal of Colorectal Disease
https://www.readbyqxmd.com/read/27959617/senescence-in-copd-and-its-comorbidities
#20
Peter J Barnes
Chronic obstructive pulmonary disease (COPD) is regarded as a disease of accelerated lung aging. This affliction shows all of the hallmarks of aging, including telomere shortening, cellular senescence, activation of PI3 kinase-mTOR signaling, impaired autophagy, mitochondrial dysfunction, stem cell exhaustion, epigenetic changes, abnormal microRNA profiles, immunosenescence, and a low-grade chronic inflammation (inflammaging). Many of these pathways are driven by chronic exogenous and endogenous oxidative stress...
December 9, 2016: Annual Review of Physiology
keyword
keyword
56266
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"