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https://www.readbyqxmd.com/read/28939530/towards-the-modulation-of-oxidative-damage-apoptosis-and-tight-junction-protein-in-response-to-dietary-leucine-deficiency-a-likely-cause-of-ros-induced-gill-structural-integrity-impairment
#1
Wei-Dan Jiang, Yu-Ping Deng, Xiao-Qiu Zhou, Yang Liu, Jun Jiang, Sheng-Yao Kuang, Ling Tang, Wu-Neng Tang, Pei Wu, Yong-An Zhang, Lin Feng
The current study explored the protective effect of leucine on antioxidant status, apoptosis and tight junction damage in the gill of grass carp (Ctenopharyngodon idella Val.). The trial was conducted by feeding grass carp with six graded level of leucine (7.1, 8.9, 11.0, 13.3, 15.2 and 17.1 g kg(-1) diet) for 8 weeks. The fish were fed to apparent satiation 4 times per day. The results indicated that compared with the leucine deficiency group, 8.9-11.3 g leucine kg(-1) diet supplementations decreased protein carbonyl (PC), malondialdehyde (MDA) and ROS contents, which may be partially attributed to the improvement of antioxidant status in the gill by increasing hydroxyl radical capacity and anti-superoxide radicals, glutathione contents and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), glutathione peroxidase (GPx), catalase (CAT), glutathione-S-transferase (GST) and glutathione reductase (GR), that referring to the up-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA expression...
September 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28928456/enabling-stop-codon-read-through-translation-in-bacteria-as-a-probe-for-amyloid-aggregation
#2
Laura Molina-García, Rafael Giraldo
Amyloid aggregation of the eukaryotic translation terminator eRF3/Sup35p, the [PSI (+)] prion, empowers yeast ribosomes to read-through UGA stop codons. No similar functional prion, skipping a stop codon, has been found in Escherichia coli, a fact possibly due to the efficient back-up systems found in bacteria to rescue non-stop complexes. Here we report that engineering hydrophobic amyloidogenic repeats from a synthetic bacterial prion-like protein (RepA-WH1) into the E. coli releasing factor RF1 promotes its aggregation and enables ribosomes to continue with translation through a premature UAG stop codon located in a β-galactosidase reporter...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28924369/il-12-influence-mtor-to-modulate-cd8-t-cells-differentiation-through-t-bet-and-eomesodermin-in-response-to-invasive-pulmonary-aspergillosis
#3
Hao Wang, Jingdong Li, Qiyang Han, Fei Yang, Yu Xiao, Meng Xiao, Yingchun Xu, Longxiang Su, Na Cui, Dawei Liu
Objective: To investigate whether mTOR signaling pathway regulate the proliferation and differentiation of CD8(+) T cells by transcription factors T-bet and Eomes, and explore the role of IL-12 in this biological procedure. Methods: Aspergillus fumigatus spore suspension nasal inhalation was used to establish the invasive pulmonary aspergillosis (IPA) mouse model. After inoculation, rapamycin (2mg/kg) each day or IL-12 (5ug/kg) every other day was given for 7 days. The blood samples were obtained before the mice sacrificed and lung specimens were taken...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28902565/molecular-cloning-and-characterization-of-ribosomal-protein-s9-in-echinococcus-granulosus
#4
Limei Wen, Guodong Lü, Shuai Lu, Huijing Gao, Bei Chen, Yunfang Ma, Yuan Yuan, Haibo Zhang, Yunfeng Xiao, Hui Liu, Jun Zhao, Jianhua Wang
Ribosomal protein S9 (RPS9) is an essential functional gene that participate in DNA repair and developmental regulations. A sequence homolog of RPS9 has been found to be upregulated in the protoscoleces (PSCs) of Echinococcus granulosus treated with artemisinin (ART). However, Echinococcus granulosus RPS9 (EgRPS9) has not been identified before. In the present study, the 657 bp cDNA encoding EgRPS9 was cloned. Amino acid sequence analysis showed that EgRPS9 was similar to the RSP9 proteins from Schistosoma japonicum (SjRPS9, 86 %) and Schistosoma mansoni (SmRPS9, 79 %)...
September 13, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28900896/fusion-of-gelonin-and-anti-insulin-like-growth-factor-1-receptor-igf-1r-affibody-for-enhanced-brain-cancer-therapy
#5
Songhee Ham, Kyoung Ah Min, Jae Wook Yang, Meong Cheol Shin
Owing to the extraordinary potency in inhibiting protein translation that could eventually lead to apoptosis of tumor cells, ribosome-inactivating proteins (RIPs) such as gelonin have been considered attractive drug candidates for cancer therapy. However, due to several critical obstacles (e.g., severe toxicity issues caused by a lack of selectivity in their mode of action and the low cytotoxicity via poor cellular uptake, etc.), clinical application of RIPs is yet far from being accomplished. To overcome these challenges, in the present study, we engineered gelonin fusion proteins with anti-insulin-like growth factor-1 receptor (IGF-1R) affibody ("IAFF") via the genetic recombinant method and the SpyCatcher/SpyTag-mediated conjugation method...
September 12, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28882678/rna-interference-mediated-knockdown-of-sirt1-and-or-sirt2-in-melanoma-identification-of-downstream-targets-by-large-scale-proteomics-analysis
#6
Melissa J Wilking-Busch, Mary A Ndiaye, Xiaoqi Liu, Nihal Ahmad
Melanoma is the most notorious and fatal of all skin cancers and the existing treatment options have not been proven to effectively manage this neoplasm, especially the metastatic disease. Sirtuin (SIRT) proteins have been shown to be differentially expressed in melanoma. We have shown that SIRTs 1 and 2 were overexpressed in melanoma and inhibition of SIRT1 imparts anti-proliferative responses in human melanoma cells. To elucidate the impact of SIRT 1 and/or 2 in melanoma, we created stable knockdowns of SIRTs 1, 2, and their combination using shRNA mediated RNA interference in A375 human melanoma cells...
September 4, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28882191/effects-of-sustained-i-c-v-infusion-of-lupus-csf-and-autoantibodies-on-behavioral-phenotype-and-neuronal-calcium-signaling
#7
Minesh Kapadia, Dunja Bijelić, Hui Zhao, Donglai Ma, Ljudmila Stojanovich, Milena Milošević, Pavle Andjus, Boris Šakić
Systemic lupus erythematosus (SLE) is a potentially fatal autoimmune disease that is often accompanied by brain atrophy and diverse neuropsychiatric manifestations of unknown origin. More recently, it was observed that cerebrospinal fluid (CSF) from patients and lupus-prone mice can be neurotoxic and that acute administration of specific brain-reactive autoantibodies (BRAs) can induce deficits in isolated behavioral tasks. Given the chronic and complex nature of CNS SLE, the current study examines broad behavioral performance and neuronal Ca(2+) signaling in mice receiving a sustained infusion of cerebrospinal fluid (CSF) from CNS SLE patients and putative BRAs (anti-NR2A, anti-ribosomal P, and anti-α-tubulin)...
September 7, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28874379/antagonism-between-front-line-antibiotics-clarithromycin-and-amikacin-used-for-the-treatment-of-mycobacterium-abscessus-infections-is-mediated-by-the-whib7-gene
#8
Mark Pryjma, Jan Burian, Kevin Kuchinski, Charles J Thompson
Combinations of antibiotics, each individually effective against Mycobacterium abscessus, are routinely co-administered based on the concept that this minimizes the spread of antibiotic resistance. However, our in vitro data contradicts this assumption and instead documents antagonistic interactions between two antibiotics (clarithromycin and amikacin) used to treat M. abscessus infections. Clinically relevant concentrations of clarithromycin induced increased resistance to both amikacin and itself. Induction of resistance was dependent on whiB7, a transcriptional activator of intrinsic antibiotic resistance that is induced by exposure to many different antibiotics...
September 5, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28873013/involvement-of-the-specific-nucleolar-protein-surf6-in-regulation-of-proliferation-and-ribosome-biogenesis-in-mouse-nih-3t3-fibroblasts
#9
Anastasiia Moraleva, Charalambos Magoulas, Mikhail Polzikov, Sabine Hacot, Hichem C Mertani, Jean-Jacques Diaz, Olga Zatsepina
The nucleolar proteins which link cell proliferation to ribosome biogenesis are regarded to be potentially oncogenic. Here, in order to examine the involvement of an evolutionary conserved nucleolar protein SURF6/Rrp14 in proliferation and ribosome biogenesis in mammalian cells, we established stably transfected mouse NIH/3T3 fibroblasts capable of conditional overexpression of the protein. Cell proliferation was monitored in real-time, and various cell cycle parameters were quantified based on flow cytometry, Br-dU-labeling and conventional microscopy data...
September 5, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28827731/microrna-7641-is-a-regulator-of-ribosomal-proteins-and-a-promising-targeting-factor-to-improve-the-efficacy-of-cancer-therapy
#10
Abu Musa Md Talimur Reza, Yun-Jung Choi, Yu-Guo Yuan, Joydeep Das, Hideyo Yasuda, Jin-Hoi Kim
Many diseases, including myocardial infarction, autoimmune disease, viral diseases, neurodegenerative diseases, and cancers, are frequently diagnosed with aberrant expression of microRNAs (miRNAs) and their allied pathways. This indicates the crucial role of miRNAs in maintaining biological and physiological processes. miR-7641 is a miRNA whose role in disease has not been fully investigated. In the present study, we investigated the expression pattern of miR-7641 and its target genes in different cancer cells, as well as in clinical cancer patients...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28820895/identification-of-leishmania-donovani-antigen-in-circulating-immune-complexes-of-visceral-leishmaniasis-subjects-for-diagnosis
#11
Fauzia Jamal, Pushkar Shivam, Sarita Kumari, Manish Kumar Singh, Abul Hasan Sardar, Pushpanjali, Selvasankar Murugesan, Shyam Narayan, Anil Kumar Gupta, Krishna Pandey, V N R Das, Vahab Ali, Sanjiva Bimal, Pradeep Das, Shubhankar K Singh
The unreliability of most of the existing antibody-based diagnostic kits to discriminate between active and treated VL cases, relapse situation and reinfection are a major hurdle in controlling the cases of Kala-azar in an endemic area. An antigen targeted diagnostic approaches can be an attractive strategy to overcome these problems. Hence, this study was focused on identifying the Leishmania antigens, lies in circulating immune complex (CICs), can be used for diagnostic as well as prognostic purposes. The present study was conducted on peripheral blood samples of 115 human subjects, based on isolation of CICs...
2017: PloS One
https://www.readbyqxmd.com/read/28818463/ribosomally-synthesised-cyclic-peptides-from-plants-as-drug-leads-and-pharmaceutical-scaffolds
#12
David J Craik, Meng-Han Lee, Fabian B H Rehm, Benjamin Tombling, Benjamin Doffek, Hayden Peacock
Owing to their exceptional stability and favourable pharmacokinetic properties, plant-derived cyclic peptides have recently attracted significant attention in the field of peptide-based drug design. This article describes the three major classes of ribosomally-synthesised plant peptides - the cyclotides, the PawS-derived peptides and the orbitides - and reviews their applications as leads or scaffolds in drug design. These ribosomally-produced peptides have a range of biological activities, including anti-HIV, cytotoxic and immunomodulatory activity...
August 8, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28808038/the-phosphatidylinositol-3-kinase-pathway-as-a-potential-therapeutic-target-in-bladder-cancer
#13
Shuxiong Zeng, Yanjun Zhu, Ai-Hong Ma, Weimin Yu, Hongyong Zhang, Tzu-Yin Lin, Wei Shi, Clifford G Tepper, Paul T Henderson, Susan Airhart, Jianming Guo, Chuanliang Xu, Ralph de Vere White, Chong-Xian Pan
PURPOSE: Activation of the phosphatidylinositol 3-kinase (PI3K) pathway occurs in over 40% of bladder urothelial cancers. The aim of this study is to determine the therapeutic potential, the underlying action and resistant mechanisms of drugs targeting the PI3K pathway. EXPERIMENTAL DESIGN: Urothelial cancer cell lines and patient-derived xenografts (PDXs) were analyzed for alterations of the PI3K pathway and for their sensitivity to the small molecule inhibitor pictilisib alone and in combination with cisplatin and/or gemcitabine...
August 14, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28801230/a-mitochondrial-proteomics-view-of-complex-i-deficiency-in-candida-albicans
#14
Xiaodong She, Pengyi Zhang, Ying Gao, Lulu Zhang, Qiong Wang, Hui Chen, Richard Calderone, Weida Liu, Dongmei Li
Proteomic analyses were carried out on isolated mitochondrial samples of C. albicans from gene-deleted mutants (nuo1Δ, nuo2Δ and goa1Δ) as well as the parental strain in order to better understand the contribution of these three fungal-specific mitochondrial ETC complex I (CI) subunits to cellular activities. Herein, we identify 2333 putative proteins from four strains, in which a total of 663 proteins (28.5%) are putatively located in mitochondria. Comparison of protein abundances between mutants and the parental strain reveal 146 differentially-expressed proteins, of which 78 are decreased and 68 are increased in at least one mutant...
August 8, 2017: Mitochondrion
https://www.readbyqxmd.com/read/28771025/synonymous-codon-bias-as-a-basis-for-novel-antibiotic-design-from-nucleotide-wobble-constraint-to-ribosomal-garrotte
#15
James H McKie, Samuel A McKie
AIM: The observation of low guanine frequencies at wobble position codons over Rossmann-fold GXGXXG motifs, led to the discovery of sequence-wide synonymous codon bias, from this we propose novel ribosomal inhibitors. Methodology & results: The wobble bases of multiple sequence alignments of diverse Rossmann-fold enzymes were counted, A, C, T and G wobble frequencies of consecutive codons were displayed as wobble plots. Synonymous codon constraints were found throughout the length of proteins, particularly at Rossmann folds...
August 3, 2017: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/28768938/a-novel-molecular-model-of-plant-lectin-induced-programmed-cell-death-in-cancer
#16
Zheng Shi, Wen-Wen Li, Yong Tang, Li-Jia Cheng
Plant lectin, a class of highly diverse non-immune origin and carbohydrate-binding proteins, has been reported to specially induce cancer cell through programmed cell death (PCD) pathways (apoptosis and/or autophagy), shedding lights on screening promising anti-cancer candidate agent for further therapeutic trials. However, the complicated molecular mechanisms by which plant lectins induced the programmed death of tumor cells, have not yet been fully clarified. Here, we summarized a novel model, based on vast amount of research, by which plant lectins eliminate various types of cancer cells via three major pathways, including a) direct ribosome inactivating, b) endocytosis-dependent mitochondrial dysfunction and c) sugar-containing receptors binding...
August 1, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28762275/ribosome-rescue-inhibitors-kill-actively-growing-and-nonreplicating-persister-mycobacterium-tuberculosis-cells
#17
John N Alumasa, Paolo S Manzanillo, Nicholas D Peterson, Tricia Lundrigan, Anthony D Baughn, Jeffery S Cox, Kenneth C Keiler
The emergence of Mycobacterium tuberculosis (MTB) strains that are resistant to most or all available antibiotics has created a severe problem for treating tuberculosis and has spurred a quest for new antibiotic targets. Here, we demonstrate that trans-translation is essential for growth of MTB and is a viable target for development of antituberculosis drugs. We also show that an inhibitor of trans-translation, KKL-35, is bactericidal against MTB under both aerobic and anoxic conditions. Biochemical experiments show that this compound targets helix 89 of the 23S rRNA...
September 8, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28761783/quantitative-real-time-pcr-analysis-of-anopheles-dirus-tep1-and-nos-during-plasmodium-berghei-infection-using-three-reference-genes
#18
Jonathan W K Liew, Mun Yik Fong, Yee Ling Lau
Quantitative reverse transcription PCR (qRT-PCR) has been an integral part of characterizing the immunity of Anopheles mosquitoes towards Plasmodium invasion. Two anti-Plasmodium factors of Anopheles, thioester-containing protein 1 (TEP1) and nitric oxide synthase (NOS), play a role in the refractoriness of Anopheles towards Plasmodium infection and are generally expressed during infection. However, these are less studied in Anopheles dirus, a dominant malaria vector in Southeast Asia. Furthermore, most studies used a single reference gene for normalization during gene expression analysis without proper validation...
2017: PeerJ
https://www.readbyqxmd.com/read/28754881/sp110-enhances-macrophage-resistance-to-mycobacterium-tuberculosis-via-inducing-endoplasmic-reticulum-stress-and-inhibiting-anti-apoptotic-factors
#19
Yongyan Wu, Zekun Guo, Fayang Liu, Kezhen Yao, Mingqing Gao, Yan Luo, Yong Zhang
Tuberculosis remains a leading health problem worldwide and still accounts for about 1.3 million deaths annually. Expression of the mouse Sp110 nuclear body protein (Sp110) upregulates the apoptotic pathway, which plays an essential role in enhancing host immunity to Mycobacterium tuberculosis (Mtb). However, the mechanism of this upregulation is unclear. Here, we have identified 253 proteins in mouse macrophages that interact with Sp110, of which 251 proteins were previously uncharacterized. The results showed that Sp110 interacts with heat shock protein 5 (Hspa5) to activate endoplasmic reticulum (ER) stress-induced apoptosis, and that this is essential for Sp110 enhanced macrophage resistance to Mtb...
July 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28754844/mitochondrial-markers-predict-recurrence-metastasis-and-tamoxifen-resistance-in-breast-cancer-patients-early-detection-of-treatment-failure-with-companion-diagnostics
#20
Federica Sotgia, Marco Fiorillo, Michael P Lisanti
Here, we used a data-mining and informatics approach to discover new biomarkers of resistance to hormonal therapy in breast cancer. More specifically, we investigated whether nuclear-encoded genes associated with mitochondrial biogenesis can be used to predict tumor recurrence, distant metastasis and treatment failure in high-risk breast cancer patients. Overall, this strategy allowed us to directly provide in silico validation of the prognostic value of these mitochondrial components in large and clinically relevant patient populations, with >15 years of follow-up data...
July 27, 2017: Oncotarget
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