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Macrophage GM-CSF

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https://www.readbyqxmd.com/read/29776615/a-new-and-efficient-culture-method-for-porcine-bone-marrow-derived-m1-and-m2-polarized-macrophages
#1
Jiye Gao, Maaike R Scheenstra, Albert van Dijk, Edwin J A Veldhuizen, Henk P Haagsman
BACKGROUND: Macrophages play an important role in the innate immune system as part of the mononuclear phagocyte system (MPS). They have a pro-inflammatory signature (M1-polarized macrophages) or anti-inflammatory signature (M2-polarized macrophages) based on expression of surface receptors and secretion of cytokines. However, very little is known about the culture of macrophages from pigs and more specific about the M1 and M2 polarization in vitro. METHODS: Porcine monocytes or mononuclear bone marrow cells were used to culture M1- and M2-polarized macrophages in the presence of GM-CSF and M-CSF, respectively...
June 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29771686/spleen-mediates-a-distinct-hematopoietic-progenitor-response-supporting-tumor-promoting-myelopoiesis
#2
Chong Wu, Huiheng Ning, Mingyu Liu, Jie Lin, Shufeng Luo, Wenjie Zhu, Jing Xu, Wen-Chao Wu, Jing Liang, Chun-Kui Shao, Jiaqi Ren, Bin Wei, Jun Cui, Min-Shan Chen, Limin Zheng
Cancer progression is associated with alterations of intra- and extramedullary hematopoiesis to support a systemic tumor-promoting myeloid response. However, the functional specialty, mechanism, and clinical relevance of extramedullary hematopoiesis (EMH) remain unclear. Here we showed that the heightened splenic myelopoiesis in tumor-bearing hosts was not only characterized by the accumulation of myeloid precursors, but also associated with profound functional alterations of splenic early hematopoietic stem/progenitor cells (HSPCs)...
May 17, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29771377/alternative-splicing-analysis-in-human-monocytes-and-macrophages-reveals-mbnl1-as-major-regulator
#3
Hongfei Liu, Paolo A Lorenzini, Fan Zhang, Shaohai Xu, Mei Su M Wong, Jie Zheng, Xavier Roca
We report the detailed transcriptomic profiles of human innate myeloid cells using RNA sequencing. Monocytes migrate from blood into infected or wounded tissue to differentiate into macrophages, and control inflammation via phagocytosis or cytokine secretion. We differentiated culture primary monocytes with either GM- or M-CSF to obtain pro- or anti-inflammatory macrophages, and respectively activated them with either LPS/IFNγ or anti-inflammatory cytokines. We also treated the THP-1 monocytic cell line with PMA and similar cytokines to mimic differentiation and activation...
May 16, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29769377/-ex-vivo-cytokine-release-determined-by-a-multiplex-cytokine-assay-in-response-to-coccidioidal-antigen-stimulation-of-whole-blood-among-subjects-with-recently-diagnosed-primary-pulmonary-coccidioidomycosis
#4
Neil M Ampel, Ian Robey, Chinh T Nguyen, Brentin Roller, Jessica August, Kenneth S Knox, Demosthenes Pappagianis
The elements of the cellular immune response in human coccidioidomycosis remain undefined. We examined the ex vivo release of an array of inflammatory proteins in response to incubation with a coccidioidal antigen preparation to ascertain which of these might be associated with diagnosis and outcome. Patients with a recent diagnosis of primary pulmonary coccidioidomycosis and a control group of healthy subjects were studied. Blood samples were incubated for 18 h with T27K, a soluble coccidioidal preparation containing multiple glycosylated antigens, and the supernatant was assayed for inflammatory proteins using the multiplex Luminex system...
June 27, 2018: MSphere
https://www.readbyqxmd.com/read/29765083/adeno-associated-virus-vector-mediated-interleukin-10-induction-prevents-vascular-inflammation-in-a-murine-model-of-kawasaki-disease
#5
Jun Nakamura, Sachiko Watanabe, Hiroaki Kimura, Motoi Kobayashi, Tadayoshi Karasawa, Ryo Kamata, Fumitake Usui-Kawanishi, Ai Sadatomo, Hiroaki Mizukami, Noriko Nagi-Miura, Naohito Ohno, Tadashi Kasahara, Seiji Minota, Masafumi Takahashi
Kawasaki disease (KD), which is the leading cause of pediatric heart disease, is characterized by coronary vasculitis and subsequent aneurysm formation. Although intravenous immunoglobulin therapy is effective for reducing aneurysm formation, a certain number of patients are resistant to this therapy. Because interleukin-10 (IL-10) was identified as a negative regulator of cardiac inflammation in a murine model of KD induced by Candida albicans water-soluble fraction (CAWS), we investigated the effect of IL-10 supplementation in CAWS-induced vasculitis...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29746488/development-of-endocytosis-degradative-activity-and-antigen-processing-capacity-during-gm-csf-driven-differentiation-of-murine-bone-marrow
#6
Adesola C Olatunde, Laura P Abell, Ashley E Landuyt, Elizabeth Hiltbold Schwartz
Dendritic cells (DC) are sentinels of the immune system, alerting and enlisting T cells to clear pathogenic threats. As such, numerous studies have demonstrated their effective uptake and proteolytic activities coupled with antigen processing and presentation functions. Yet, less is known about how these cellular mechanisms change and develop as myeloid cells progress from progenitor cells to more differentiated cell types such as DC. Thus, our study comparatively examined these functions at different stages of myeloid cell development driven by the GM-CSF...
2018: PloS One
https://www.readbyqxmd.com/read/29745908/local-use-of-granulocyte-macrophages-colony-stimulating-factor-in-treatment-of-chronic-diabetic-neuropathic-ulcer-case-review
#7
E Karlafti, Ch Savopoulos, A Hatzitolios, T Didangelos
One of the main causes of amputations in patients with Diabetes Mellitus patients is a chronic diabetic foot ulcer. The authors present a clinical case and discussion of a successful use of Granulocyte-Macrophages Colony Stimulating Factor (GM-CSF) treatment for the promotion of healing of a chronic diabetic foot ulcer. A 65 year-old woman was admitted to the Diabetes Center with complaints of a deep non-healing chronic foot ulcer for the last 18 months. At the examination a 5 cm ulcer on the plantar surface of the right foot was revealed...
April 2018: Georgian Medical News
https://www.readbyqxmd.com/read/29743313/ivig-promote-cross-tolerance-against-inflammatory-stimuli-in-vitro-and-in-vivo
#8
Ángeles Domínguez-Soto, Miriam Simón-Fuentes, Mateo de Las Casas-Engel, Víctor D Cuevas, María López-Bravo, Jorge Domínguez-Andrés, Paula Saz-Leal, David Sancho, Carlos Ardavín, Juliana Ochoa-Grullón, Silvia Sánchez-Ramón, Miguel A Vega, Angel L Corbí
IVIg is an approved therapy for immunodeficiency and for several autoimmune and inflammatory diseases. However, the molecular basis for the IVIg anti-inflammatory activity remains to be fully explained and cannot be extrapolated from studies on animal models of disease. We now report that IVIg impairs the generation of human monocyte-derived anti-inflammatory macrophages by inducing JNK activation and activin A production and limits proinflammatory macrophage differentiation by inhibiting GM-CSF-driven STAT5 activation...
May 9, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29737428/the-ratios-of-pro-inflammatory-to-anti-inflammatory-cytokines-in-the-serum-of-chronic-periodontitis-patients-with-and-without-type-2-diabetes-and-or-smoking-habit
#9
Tamires Szeremeske Miranda, Sílvia Lacerda Heluy, Daniele Ferreira Cruz, Hélio Doyle Pereira da Silva, Magda Feres, Luciene Cristina Figueiredo, Poliana Mendes Duarte
OBJECTIVE: This study assessed the impact of chronic periodontitis (CP) and CP associated with type 2 diabetes mellitus (DM) and/or smoking on the serum ratios of pro- to anti-inflammatory cytokines. MATERIALS AND METHODS: Subjects were assigned into one of the following groups: control (n = 25, non-diabetic non-smokers with no history of periodontitis), CP (n = 26, non-diabetic non-smokers with CP), DMCP (n = 30, non-smokers with DM and CP), SCP (n = 27, non-diabetic smokers with CP), and SDMCP (n = 22, smokers with type 2 DM and CP)...
May 8, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29736292/extended-culture-of-bone-marrow-with-granulocyte-macrophage-colony-stimulating-factor-generates-immunosuppressive-cells
#10
Hye Young Na, Moah Sohn, Seul Hye Ryu, Wanho Choi, Hyunju In, Hyun Soo Shin, Chae Gyu Park
Bone marrow-derived dendritic cells (BM-DCs) are generated from bone marrow (BM) cells cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) for a week. In this study we investigated the effect of duration on the BM culture with GM-CSF. Within several months, the cells in the BM culture gradually expressed homogeneous levels of CD11c and major histocompatibility complex II on surface, and they became unable to stimulate allogeneic naïve T cells in mixed lymphocyte reaction (MLR). In addition, when the BM culture were sustained for 32 wk or longer, the BM cells acquired ability to suppress the proliferation of allogeneic T cells in MLR as well as the response of ovalbumin-specific OT-I transgenic T cells in antigen-dependent manner...
April 2018: Immune Network
https://www.readbyqxmd.com/read/29730395/profiling-inflammatory-signatures-of-schizophrenia-a-cross-sectional-and-meta-analysis-study
#11
Dorota Frydecka, Małgorzata Krzystek-Korpacka, Alba Lubeiro, Filip Stramecki, Bartłomiej Stańczykiewicz, Jan Aleksander Beszłej, Patryk Piotrowski, Kamila Kotowicz, Monika Szewczuk-Bogusławska, Edyta Pawlak-Adamska, Błażej Misiak
We aimed to profile a broad panel of inflammatory markers in patients with schizophrenia and healthy controls. Additionally, we performed a meta-analysis of chemokine alterations that have not been subjected to quantitative synthesis so far. We recruited 78 patients with schizophrenia and 78 healthy controls, and measured inflammatory markers using the Luminex technology. After adjustment for multiple testing, we found elevated levels of interleukin (IL)-1 receptor antagonist (IL-1RA), IL-6, IL-7, IL-8, IL-9, IL-10, IL-13, interferon-γ, eotaxin-1, granulocyte-macrophage colony-stimulating factor (GM-CSF), monocyte chemoattractant protein-1 (MCP-1), platelet-derived growth factor with two B subunits (PDGF-BB), macrophage inflammatory protein (MIP)-1α, MIP-1β, vascular endothelial growth factor A (VEGF-A) and RANTES in multiple-episode schizophrenia (MES) patients...
May 3, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29720827/anti-inflammatory-effects-of-brassica-oleracea-var-capitata-l-cabbage-methanol-extract-in-mice-with-contact-dermatitis
#12
Youjung Lee, Seoyoung Kim, Beodeul Yang, Chiyeon Lim, Jung-Hoon Kim, Hyungwoo Kim, Suin Cho
Background: Cabbage, Brassica oleracea var. capitata L., is one of the most common vegetables in the world. Because of its high levels of flavonoids and anthocyanins, cabbage has long been used as a herbal medicine. The antioxidant and anti-inflammatory properties of cabbage were also recently been reported. Objective: This study was designed to investigate the anti-inflammatory effects of cabbage in mice with contact dermatitis (CD). Materials and Methods: We investigated the effects of methanol extract of B...
April 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29719809/autoimmune-pulmonary-alveolar-proteinosis-in-an-adolescent-successfully-treated-with-inhaled-rhgm-csf-molgramostim
#13
Marta E Gajewska, Sajitha S Sritharan, Eric Santoni-Rugiu, Elisabeth M Bendstrup
Autoimmune pulmonary alveolar proteinosis (aPAP) is a rare parenchymal lung disease characterized by accumulation of surfactant in the airways with high levels of granulocyte-macrophage colony stimulating factor (GM-CSF) antibodies in blood. Disease leads to hypoxemic respiratory failure. Whole lung lavage (WLL) is considered the first line therapy, but procedure can be quite demanding, specifically for children. Recently alternative treatment options with inhaled GM-CSF have been described but no consensus about the standard treatment exists...
2018: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/29713367/wentong-decoction-cures-allergic-bronchial-asthma-by-regulating-the-apoptosis-imbalance-of-eos
#14
Yue Yan, Hai-Peng Bao, Chun-Lei Li, Qi Shi, Yan-Hua Kong, Ting Yao, You-Lin Li
Background: Eosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma. Therefore, this study aimed to observe the effect of Chinese medicine Wentong decoction (WTD) in EOS apoptosis in asthmatic rats. This work also explored the mechanism of WTD regulation in EOS apoptosis and provided a new target for clinical treatment of asthma. Methods: Asthmatic rats induced by ovalbumin were treated with WTD...
2018: Chinese Medicine
https://www.readbyqxmd.com/read/29704458/gm-csf-and-il-4-are-not-involved-in-ivig-mediated-amelioration-of-itp-in-mice-a-role-for-il-11-cannot-be-ruled-out
#15
Bonnie J B Lewis, Danila Leontyev, Anton Neschadim, Megan Blacquiere, Donald R Branch
Previously, we reported that interleukin 4 (IL-4), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin 11 (IL-11), but not interleukin 33 (IL-33), are up-regulated in two strains of mice with immune thrombocytopenia (ITP) that are responsive to intravenous immunoglobulin (IVIg) treatment. Previously, IL-4 was ruled out in the mechanism of IVIg; however, other publications have suggested this cytokine as a major player in the mechanism of IVIg action. Thus, we sought to further investigate a role for IL-4 and, in addition, GM-CSF and IL-11 in the mechanism of action of IVIg using a murine model of ITP...
April 28, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29701676/anti-inflammatory-effect-of-baicalein-on-polyinosinic%C3%A2-polycytidylic-acid-induced-raw-264-7-mouse-macrophages
#16
Young-Jin Kim, Hyun-Ju Kim, Ji Young Lee, Do-Hoon Kim, Mi Suk Kang, Wansu Park
Baicalein (3,3′,4′,5,6-pentahydroxyflavone) is a well-known antioxidant found in many plants, such as in the roots of Scutellaria baicalensis . In this study, we evaluate the inhibitory effect of baicalein on the inflammatory cascade in RAW 264.7 mouse macrophages induced by viral-like material. Experimental assays used in this study included Griess reagent assay for nitric oxide (NO) production, Fluo-4 assay for intracellular calcium release, multiplex cytokine assay, and quantitative real time RT-PCR assay...
April 26, 2018: Viruses
https://www.readbyqxmd.com/read/29682323/granulocyte-macrophage-colony-stimulating-factor-negatively-regulates-early-il-10-mediated-responses
#17
Ruud Hp Wilbers, Lotte B Westerhof, Debbie R van Raaij, Jaap Bakker, Geert Smant, Arjen Schots
Aim: Treatment of inflammatory disorders relies on the intervention in immune responses thereby restoring homeostasis. IL-10 is a cytokine with therapeutic potential, but until now has not been as successful as previously anticipated. A reason for this may be that IL-10 responsiveness depends on the environment of the inflamed tissue. In this study we investigated whether GM-CSF is able to influence IL-10-mediated responses. Methodology: Dendritic cells and macrophages were differentiated from mouse bone marrow and treated or depleted from GM-CSF prior to analyze their response to IL-10...
April 2018: Future Science OA
https://www.readbyqxmd.com/read/29677388/engulfment-of-hb-activated-platelets-differentiates-monocytes-into-pro-inflammatory-macrophages-in-pnh-patients
#18
Rashi Singhal, Sheetal Chawla, Harish Batra, Sameer Gupta, Amrita Ojha, Deepak K Rathore, Tulika Seth, Prasenjit Guchhait
The distinct response shown by different phenotypes of macrophages and monocytes under various clinical conditions have put the heterogeneity of these cells into focus of investigation for several diseases. Recently we have described that after engulfing Hb-activated platelets, classical monocytes differentiated into pro-inflammatory phenotypes, which were abundant in the circulation of PNH and SCD patients. Our current study shows that upon engulfment of Hb-activated platelets, monocytes differentiate into M1-macrophages under M1-polarization stimulus (GM-CSF, IFN-γ+LPS)...
April 20, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29669721/ppar-%C3%AE-contributes-to-immunity-by-cancer-vaccines-that-secrete-gm-csf
#19
Girija Goyal, Karrie Wong, Christopher J Nirschl, Nicholas Souders, Donna Neuberg, Niroshana Anandasabapathy, Glenn Dranoff
Peroxisome proliferator activated receptor-γ (PPARγ) is a lipid-activated nuclear receptor that promotes immune tolerance through effects on macrophages, dendritic cells (DCs), and regulatory T cells (Tregs). Granulocyte-macrophage colony stimulating factor (GM-CSF) induces PPARγ expression in multiple myeloid cell types. GM-CSF contributes to both immune tolerance and protection, but the role of PPARγ in these pathways is poorly understood. Here we reveal an unexpected stimulatory role for PPARγ in the generation of antitumor immunity with irradiated, GM-CSF-secreting tumor-cell vaccines (GVAX)...
April 18, 2018: Cancer Immunology Research
https://www.readbyqxmd.com/read/29669585/endoplasmic-reticulum-stress-in-perivascular-adipose-tissue-promotes-destabilization-of-atherosclerotic-plaque-by-regulating-gm-csf-paracrine
#20
Ru Ying, Sheng-Wei Li, Jia-Yuan Chen, Hai-Feng Zhang, Ying Yang, Zhen-Jie Gu, Yang-Xin Chen, Jing-Feng Wang
BACKGROUND: Perivascular adipose tissue (PVAT) accelerates plaque progression and increases cardiovascular risk. We tested the hypothesis that PVAT contributed to plaque vulnerability and investigated whether endoplasmic reticulum stress (ER stress) in PVAT played an important role in vulnerable plaque. METHODS: We transplanted thoracic aortic PVAT or subcutaneous adipose tissue as a control, from donor mice to carotid arteries of recipient apolipoprotein E deficient (apoE-/- ) mice after removing carotid artery collar placed for 6 weeks...
April 18, 2018: Journal of Translational Medicine
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