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integrin beta6

Kate M Moore, Gareth J Thomas, Stephen W Duffy, Jane Warwick, Rhian Gabe, Patrick Chou, Ian O Ellis, Andrew R Green, Syed Haider, Kellie Brouilette, Antonio Saha, Sabari Vallath, Rebecca Bowen, Claude Chelala, Diana Eccles, William J Tapper, Alastair M Thompson, Phillip Quinlan, Lee Jordan, Cheryl Gillett, Adam Brentnall, Shelia Violette, Paul H Weinreb, Jane Kendrew, Simon T Barry, Ian R Hart, J Louise Jones, John F Marshall
BACKGROUND: Integrin αvβ6 promotes migration, invasion, and survival of cancer cells; however, the relevance and role of αvβ6 has yet to be elucidated in breast cancer. METHODS: Protein expression of integrin subunit beta6 (β6) was measured in breast cancers by immunohistochemistry (n > 2000) and ITGB6 mRNA expression measured in the Molecular Taxonomy of Breast Cancer International Consortium dataset. Overall survival was assessed using Kaplan Meier curves, and bioinformatics statistical analyses were performed (Cox proportional hazards model, Wald test, and Chi-square test of association)...
August 2014: Journal of the National Cancer Institute
Pradyot Dash, Paul V Barnett, Michael S Denyer, Terry Jackson, Catrina M A Stirling, Philippa C Hawes, Jennifer L Simpson, Paul Monaghan, Haru-H Takamatsu
Three-dimensional (3D) porcine nasal mucosal and tracheal mucosal epithelial cell cultures were developed to analyze foot-and-mouth disease virus (FMDV) interactions with mucosal epithelial cells. The cells in these cultures differentiated and polarized until they closely resemble the epithelial layers seen in vivo. FMDV infected these cultures predominantly from the apical side, primarily by binding to integrin alphav beta6, in an Arg-Gly-Asp (RGD)-dependent manner. However, FMDV replicated only transiently without any visible cytopathic effect (CPE), and infectious progeny virus could be recovered only from the apical side...
September 2010: Journal of Virology
Kristina Bry, Anna Hogmalm, Erica Bäckström
The role of inflammation in the pathogenesis of bronchopulmonary dysplasia (BPD) is not well understood. By using a transgenic mouse expressing the inflammatory cytokine interleukin (IL)-1beta in the lung, we have shown that perinatal expression of IL-1beta causes a BPD-like illness in infant mice. We have used this model to identify mechanisms by which inflammation causes neonatal lung injury. Increased matrix metalloproteinase (MMP)-9 activity is associated with BPD. MMP-9 deficiency worsens alveolar hypoplasia in IL-1beta-expressing newborn mice, suggesting that MMP-9 has a protective role in neonatal inflammatory lung injury...
June 2010: Seminars in Perinatology
Junzheng Du, Huiyun Chang, Shandian Gao, Shuang Xue, Guozheng Cong, Junjun Shao, Tong Lin, Zaixin Liu, Xiangtao Liu, Xuepeng Cai
In the present study, we report the sequences and characterization of the porcine integrin cDNAs encoding alphav, beta3, beta6 and beta8 subunits and compare them to those of other species. The coding sequences for the porcine alphav, beta3, beta6 and beta8 subunits were found to be 3141, 2289, 2367 and 2304 nucleotides in length, encoding 1046, 762, 788 and 767-amino-acid-residue protein, respectively. The porcine integrin alphav, beta3, beta6 and beta8 subunit shares common structural and functional elements with their counterparts from the other species...
October 2010: Molecular and Cellular Probes
Shinichi Takatsuki, Rina Nakamura, Youichi Haga, Kazumasa Mitsui, Takuji Hashimoto, Keiko Shimojima, Tsutomu Saji, Toshiyuki Yamamoto
Owing to the large size of chromosome 2, partial monosomy of the long arm of this chromosome gives rise to many specific phenotypes. We report on a 2-month-old girl with an interstitial deletion of 2q24.2q24.3, which was confirmed by microarray-based comparative genomic hybridization analysis. The patient showed delayed growth and mental retardation, early myoclonic seizures, and characteristic dysmorphic features including thick arched eyebrows, upslanting palpebral fissures, long eyelashes, depressed nasal bridge, short nose, long philtrum, small mouth, micrognathia, and low set ears...
April 2010: American Journal of Medical Genetics. Part A
E Patsenker, L Wilkens, V Banz, C H Osterreicher, R Weimann, S Eisele, A Keogh, D Stroka, A Zimmermann, F Stickel
BACKGROUND & AIMS: Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC) are common primary hepatic malignancies. Their immunohistological differentiation using specific markers is pivotal for treatment and prognosis. We found alphavbeta6 integrin strongly upregulated in biliary fibrosis, but its expression in primary and secondary liver tumours is unknown. Here, we aimed to evaluate the diagnostic applicability of alphavbeta6 integrin in differentiating primary liver cancers. METHODS: Expression of alphavbeta6 integrin was evaluated in liver tissues from patients with CC, HCC, fibrolamellar HCC, combined CC/HCC, hepatic metastases of colorectal and pancreatic carcinomas, primary sclerosing cholangitis (PSC), and in human primary and tumour-derived liver cell lines by immunohisto- and cytochemistry, and by TaqMan PCR...
March 2010: Journal of Hepatology
Outi Heikkilä, Petri Susi, Tuire Tevaluoto, Heidi Härmä, Varpu Marjomäki, Timo Hyypiä, Saija Kiljunen
Coxsackievirus A9 (CAV9) is a member of the human enterovirus B species within the Enterovirus genus of the family Picornaviridae. It has been shown to utilize alphaV integrins, particularly alphaVbeta6, as its receptors. The endocytic pathway by which CAV9 enters human cells after the initial attachment to the cell surface has so far been unknown. Here, we present a systematic study concerning the internalization mechanism of CAV9 to A549 human lung carcinoma cells. The small interfering RNA (siRNA) silencing of integrin beta6 subunit inhibited virus proliferation, confirming that alphaVbeta6 mediates the CAV9 infection...
April 2010: Journal of Virology
Junzheng Du, Magdalena Larska, Huiyun Chang, Soren Alexandersen, Xuepeng Cai
Bactrian camels can relatively easily be infected with FMDV, but dromedary camels remain resistant even to high doses of the virus. To understand the different susceptibility between the two camel species from the standpoint of viral receptors, this work reports the sequences of the dromedary camel integrin cDNAs encoding alphavbeta1 and alphavbeta6 and compare them to those of other species, especially to Bactrian camels. The complete coding sequences for the dromedary camel alphav, beta1 and beta6 subunits were found to be 3147, 2397, and 2364 nucleotides in length, encoding 1048, 798, and 787 amino acids, respectively...
May 15, 2010: Veterinary Immunology and Immunopathology
Hana Sato, Masashi Hatori, Yuriko Ando, Yuji Kurihara, Sayaka Takayama, Tatsuo Shirota, Tetsuhiko Tachikawa, Satoru Shintani
S-1, an oral fluorouracil antitumor drug, is composed of three agents: tegafur (FT), 5-chloro-2,4-dihydroxypyridine (CDHP), and potassium oxonate (Oxo). Approximately 50% of oral squamous cell carcinomas (OSCC) exhibit cervical lymph node metastasis. The extent of lymph node involvement is a major determinant in both staging and prognosis of the majority of OSCC. The purpose of this study was to examine the effect of S-1 on the metastatic potential of OSCC cells. We used orthotopic green fluorescence protein (GFP) SAS-L1, in BALB/c nu/nu mice...
October 2009: Oncology Reports
Anna Hogmalm, Dean Sheppard, Urpo Lappalainen, Kristina Bry
Pulmonary inflammation is associated with the development of bronchopulmonary dysplasia in premature infants. We have previously shown that perinatal pulmonary expression of human IL-1beta is sufficient to cause a lung disease similar to bronchopulmonary dysplasia, characterized by inflammation, impaired alveolarization, poor postnatal growth, and increased mortality in infant mice. The alphavbeta6 integrin plays a critical role in regulating inflammation in the adult lung. To study the role of the beta6 integrin subunit in neonatal inflammatory lung disease, we compared the pulmonary development in IL-1beta-expressing infant mice with wild-type or null beta6 integrin loci...
July 2010: American Journal of Respiratory Cell and Molecular Biology
Yanshuang Xie, Kai Gao, Lari Häkkinen, Hannu S Larjava
Integrin alphavbeta6 is an epithelial-specific receptor that is absent from the healthy epidermis but synthesized de novo during wound repair. However, its function in wound repair is unknown. Integrin-mediated transforming growth factor-beta1 (TGF-beta1) activation is the main activation mechanism of this key cytokine in vivo. Impaired wound healing caused by glucocorticoids is a major clinical problem and is associated with a disturbed balance of TGF-beta1 activity. Therefore, alphavbeta6 integrin-mediated regulation of TGF-beta1 activity may be involved in this process...
May 2009: Wound Repair and Regeneration
Rui Zhao, Xiang-qun Liu, Xiao-peng Wu, Yan-feng Liu, Zhao-yang Zhang, Guang-yun Yang, Sen Guo, Jun Niu, Jia-yong Wang, Ke-sen Xu
Integrins play an important role in tumor metastasis induced by vascular endothelial growth factor (VEGF). However, in the case of gastric cancer, the precise role of VEGF in regulating integrin alphavbeta6 is unclear. In this study, we found that most of the alphavbeta6 integrin-positive gastric cancer tissues were also VEGF-positive. Furthermore, when gastric carcinoma cells were exposed to VEGF, expression of alphavbeta6 integrin was up-regulated and the extracellular signal-related kinase (ERK) pathway was activated...
January 28, 2010: Cancer Letters
Junzheng Du, Huiyun Chang, Shandian Gao, Guozheng Cong, Junjun Shao, Tong Lin, Zaixin Liu, Xiangtao Liu, Xuepeng Cai
Four members of the alphav integrin family of cellular receptors, alphavbeta1, alphavbeta3, alphavbeta6, and alphavbeta8, have been identified as receptors for foot-and-mouth disease virus (FMDV) in vitro, and integrins are believed to be the receptors used to target epithelial cells in the infected animals. To analyse roles of the alphav integrins from a susceptible species as viral receptors, we have cloned sheep alphav, beta1, and beta6 integrin cDNAs and compared them to those of other species. The coding sequences for sheep integrin alphav, beta1, and beta6 were found to be 3147, 2397, and 2364 nuclotides in length, encoding 1048, 798, and 787 amino acids, respectively...
October 2009: Molecular and Cellular Probes
Dongmin Dang, Daniel M Ramos
Oral squamous cell carcinoma (SCC) is composed of a heterogeneous population of cells which range anywhere from epithelial to mesenchymal in phenotype. Several oral cancer specimens with antibodies to TRA160, a marker of pluripotent cells, were screened. Compared with the well differentiated lesions, pluripotent cells were more numerous in specimens from poorly differentiated tumors. In vitro, the expression of TRA160 was much greater in invasive oral SCC9beta6 cells compared with the poorly invasive SCC9SN or SCC9beta6D1 cells, which express a truncated beta6...
June 2009: Anticancer Research
Anurag Singh, Patricia Greninger, Daniel Rhodes, Louise Koopman, Sheila Violette, Nabeel Bardeesy, Jeff Settleman
K-ras mutations occur frequently in epithelial cancers. Using short hairpin RNAs to deplete K-Ras in lung and pancreatic cancer cell lines harboring K-ras mutations, two classes were identified-lines that do or do not require K-Ras to maintain viability. Comparing these two classes of cancer cells revealed a gene expression signature in K-Ras-dependent cells, associated with a well-differentiated epithelial phenotype, which was also seen in primary tumors. Several of these genes encode pharmacologically tractable proteins, such as Syk and Ron kinases and integrin beta6, depletion of which induces epithelial-mesenchymal transformation (EMT) and apoptosis specifically in K-Ras-dependent cells...
June 2, 2009: Cancer Cell
Davide Provasi, Marta Murcia, Barry S Coller, Marta Filizola
The beta3 integrin family members alphaIIbeta3 and alphaVbeta3 signal bidirectionally through long-range allosteric changes, including a transition from a bent unliganded-closed low-affinity state to an extended liganded-open high-affinity state. To obtain an atomic-level description of this transition in an explicit solvent, we carried out targeted molecular dynamics simulations of the headpieces of alphaIIbeta3 and alphaVbeta3 integrins. Although minor differences were observed between these receptors, our results suggest a common transition pathway in which the hybrid domain swing-out is accompanied by conformational changes within the beta3 betaA (I-like) domain that propagate through the alpha7 helix C-terminus, and are followed by the alpha7 helix downward motion and the opening of the beta6-alpha7 loop...
November 1, 2009: Proteins
Junzheng Du, Shandian Gao, Huiyun Chang, Guozheng Cong, Tong Lin, Junjun Shao, Zaixin Liu, Xiangtao Liu, Xuepeng Cai
Integrins are heterodimeric adhesion receptors that participate in a variety of cell-cell and cell-extracellular matrix protein interactions. Many integrins recognize RGD sequences displayed on extracellular matrix proteins and the exposed loops of viral capsid proteins. Four members of the alphav integrin family of cellular receptors, alphavbeta3, alphavbeta6, alphavbeta1 and alphavbeta8, have been identified as receptors for foot-and-mouth disease virus (FMDV) in vitro, and integrins are believed to be the receptors used to target epithelial cells in the infected animals...
October 15, 2009: Veterinary Immunology and Immunopathology
Lynda Coughlan, Sabari Vallath, Antonio Saha, Magdalena Flak, Iain A McNeish, Georges Vassaux, John F Marshall, Ian R Hart, Gareth J Thomas
A key impediment to successful cancer therapy with adenoviral vectors is the inefficient transduction of malignant tissue in vivo. Compounding this problem is the lack of cancer-specific targets, coupled with a shortage of corresponding high-efficiency ligands, permitting selective retargeting. The epithelial cell-specific integrin alphavbeta6 represents an attractive target for directed therapy since it is generally not expressed on normal epithelium but is upregulated in numerous carcinomas, where it plays a role in tumor progression...
July 2009: Journal of Virology
Bartosz Kempisty, Paweł Antosik, Dorota Bukowska, Marta Jackowska, Margarita Lianeri, Jedrzej M Jaśkowski, Paweł P Jagodziński
Using reverse transcription and real-time quantitative PCR analysis we evaluated the transcript levels of integrins (alphaL, alphaM, beta1, and beta6), CD9 and CD18 antigens as well as zona pellucida glycoproteins (pZP1, pZP2, pZP3 and pZP3alpha) in oocytes isolated from puberal gilts (n=20) and multiparous sows (n=20). We found significantly (p<0.05) higher transcript contents of alphaL, alphaM, beta1, and beta integrins, CD9 antigen, and pZP2 and pZP3 in puberal gilt oocytes compared to multiparous sow oocytes...
March 2009: Reproductive Biology
Daniel M Ramos, Dongmin Dang, Stephen Sadler
In this study, we evaluated whether the forced expression of beta6 integrin would modulate the epithelial to mesenchymal transition (EMT). When the full length beta6 integrin was expressed in poorly invasive squamous cell carcinoma SCC9 cells, the resulting SCC9/6 cells acquired a fibroblast-like morphology, increased expression of the mesenchymal marker vimentin and reduced expression of the epithelial markers keratin and E-cadherin. SCC9beta6D1 cells, which express a truncated form of beta6 subunit lacking the C-terminal 11 amino acids (AA), retained their epithelial morphology and did not alter vimentin or E-cadherin expression...
January 2009: Anticancer Research
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