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RUVBL1

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https://www.readbyqxmd.com/read/27746211/insights-into-specificity-redundancy-and-new-cellular-functions-of-c-ebpa-and-c-ebpb-transcription-factors-through-interactome-network-analysis
#1
Maurizio Cirilli, Oxana Bereshchenko, Olga Ermakova, Claus Nerlov
BACKGROUND: C/EBPa and C/EBPb are transcription factors with tissue specific expression regulating several important cellular processes. They work by recruiting protein complexes to a common DNA recognition motif and both are able to compensate each other's absence in many cell types, thus showing functional redundancy. They also play distinct roles in specific cellular pathways and their abnormal functioning gives raise to different human pathologies. METHODS: To investigate the molecular basis of C/EBPa and C/EBPb specificity and redundancy we characterized their in vivo protein-protein interaction networks by Tandem Affinity Purification (TAP) and Mass Spectrometry (MS)...
October 14, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27722820/downregulation-of-ruvbl1-inhibits-proliferation-of-lung-adenocarcinoma-cells-by-g1-s-phase-cell-cycle-arrest-via-multiple-mechanisms
#2
Xiao-Shuai Yuan, Zhi-Tian Wang, Ye-Ji Hu, Fei-Chao Bao, Ping Yuan, Chong Zhang, Jin-Lin Cao, Wang Lv, Jian Hu
Lung cancer remains a leading cause of cancer-related mortality and morbidity worldwide, of which non-small cell lung cancer (NSCLC) accounts for 80 %. RUVBL1 is a highly conserved eukaryotic AAA+ adenosine 5'-triphosphatase (ATPase) that has many functions highly relevant to cancer. We therefore attempted to determine the potential role of RUVBL1 in the biogenesis of lung adenocarcinoma and obtained some interesting results. Our study revealed that RUVBL1 expression was higher in lung adenocarcinoma specimens than in those of adjacent non-tumor tissues and in lung cancer cell lines than in normal lung cell lines...
October 10, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27715451/assembly-and-trafficking-of-box-c-d-and-h-aca-snornps
#3
Séverine Massenet, Edouard Bertrand, Céline Verheggen
Box C/D and box H/ACA snoRNAs are abundant non-coding RNAs that localize in the nucleolus and mostly function as guides for nucleotide modifications. While a large pool of snoRNAs modifies rRNAs, an increasing number of snoRNAs could also potentially target mRNAs. ScaRNAs belong to a family of specific RNAs that localize in Cajal bodies and that are structurally similar to snoRNAs. Most scaRNAs are involved in snRNA modification, while telomerase RNA, which contains H/ACA motifs, functions in telomeric DNA synthesis...
October 7, 2016: RNA Biology
https://www.readbyqxmd.com/read/27664947/ad-e1a-243r-oncoprotein-promotes-association-of-proto-oncogene-product-myc-with-the-nua4-tip60-complex-via-the-e1a-n-terminal-repression-domain
#4
Ling-Jun Zhao, Paul M Loewenstein, Maurice Green
The adenovirus E1A 243R oncoprotein targets TRRAP, a scaffold protein that assembles histone acetyltransferase (HAT) complexes, such as the NuA4/Tip60 complex which mediates transcriptional activity of the proto-oncogene MYC and helps determine the cancer cell phenotype. How E1A transforms cells through TRRAP remains obscure. We performed proteomic analysis with the N-terminal transcriptional repression domain of E1A 243R (E1A 1-80) and showed that E1A 1-80 interacts with TRRAP, p400, and three other members of the NuA4 complex - DMAP1, RUVBL1 and RUVBL2 - not previously shown to associate with E1A 243R...
December 2016: Virology
https://www.readbyqxmd.com/read/27250028/transcriptomic-and-proteomic-analysis-of-mouse-radiation-induced-acute-myeloid-leukaemia-aml
#5
Christophe Badie, Agnieszka Blachowicz, Zarko Barjaktarovic, Rosemary Finnon, Arlette Michaux, Hakan Sarioglu, Natalie Brown, Grainne Manning, M Abderrafi Benotmane, Soile Tapio, Joanna Polanska, Simon D Bouffler
A combined transcriptome and proteome analysis of mouse radiation-induced AMLs using two primary AMLs, cell lines from these primaries, another cell line and its in vivo passage is reported. Compared to haematopoietic progenitor and stem cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all materials. 55 and 3 alterations were detected in the proteomes of the cell lines and primary/in vivo passage material respectively, with one common to all materials. In cell lines, approximately 50% of the transcriptome changes are related to adaptation to cell culture, and in the proteome this proportion was higher...
May 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27066592/the-relationship-between-ruvbl1-pontin-tip49-nmp238-and-bcl6-in-benign-and-malignant-human-lymphoid-tissues
#6
Beverly W Baron, Rebecca M Baron, Joseph M Baron
The human BCL6 gene, which is involved in the pathogenesis of certain human lymphomas, encodes a transcriptional repressor that is needed for germinal center B cell development and T follicular helper cell differentiation. Our goal was to identify BCL6 target genes using a cell system in which BCL6 repressive effects are inhibited followed by subtractive hybridization, and we detected the RUVBL1 (Pontin, TIP49) gene as a potential target of BCL6 repression. Here we show that the BCL6 protein significantly represses RUVBL1 transcription (6...
July 2016: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/27040446/grifola-frondosa-glycoprotein-gfg-3a-arrests-s-phase-alters-proteome-and-induces-apoptosis-in-human-gastric-cancer-cells
#7
Fengjie Cui, Xinyi Zan, Yunhong Li, Wenjing Sun, Yan Yang, Lifeng Ping
GFG-3a is a novel glycoprotein previously purified from the fermented mycelia of Grifola frondosa with novel sugar compositions and protein sequencing. The present study aims to investigate its effects on the cell cycle, differential proteins expression, and apoptosis of human gastric cancer SGC-7901 cells. Our findings revealed that GFG-3a induced the cell apoptosis and arrested cell cycle at S phase. GFG-3a treatment resulted in the differential expression of 21 proteins in SGC-7901 cells by upregulating 10 proteins including RBBP4 associated with cell cycle arrest and downregulating 11 proteins including RUVBL1, NPM, HSP90AB1, and GRP78 involved in apoptosis and stress response...
2016: Nutrition and Cancer
https://www.readbyqxmd.com/read/26988609/gene-expression-profile-of-the-clinically-aggressive-micropapillary-variant-of-bladder-cancer
#8
Charles Chuanhai Guo, Vipulkumar Dadhania, Li Zhang, Tadeusz Majewski, Jolanta Bondaruk, Maciej Sykulski, Weronika Wronowska, Anna Gambin, Yan Wang, Shizhen Zhang, Enrique Fuentes-Mattei, Ashish Madhav Kamat, Colin Dinney, Arlene Siefker-Radtke, Woonyoung Choi, Keith A Baggerly, David McConkey, John N Weinstein, Bogdan Czerniak
BACKGROUND: Progression of conventional urothelial carcinoma of the bladder to a tumor with unique microscopic features referred to as micropapillary carcinoma is coupled with aggressive clinical behavior signified by a high propensity for metastasis to regional lymph nodes and distant organs resulting in shorter survival. OBJECTIVE: To analyze the expression profile of micropapillary cancer and define its molecular features relevant to clinical behavior. DESIGN, SETTING, AND PARTICIPANTS: We retrospectively identified 43 patients with micropapillary bladder cancers and a reference set of 89 patients with conventional urothelial carcinomas and performed whole-genome expression messenger RNA profiling...
March 15, 2016: European Urology
https://www.readbyqxmd.com/read/26711270/a-novel-interaction-of-ecdysoneless-ecd-protein-with-r2tp-complex-component-ruvbl1-is-required-for-the-functional-role-of-ecd-in-cell-cycle-progression
#9
Riyaz A Mir, Aditya Bele, Sameer Mirza, Shashank Srivastava, Appolinaire A Olou, Shalis A Ammons, Jun Hyun Kim, Channabasavaiah B Gurumurthy, Fang Qiu, Hamid Band, Vimla Band
Ecdysoneless (ECD) is an evolutionarily conserved protein whose germ line deletion is embryonic lethal. Deletion of Ecd in cells causes cell cycle arrest, which is rescued by exogenous ECD, demonstrating a requirement of ECD for normal mammalian cell cycle progression. However, the exact mechanism by which ECD regulates cell cycle is unknown. Here, we demonstrate that ECD protein levels and subcellular localization are invariant during cell cycle progression, suggesting a potential role of posttranslational modifications or protein-protein interactions...
March 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/26303906/ruvbl1-and-ruvbl2-enhance-aggresome-formation-and-disaggregate-amyloid-fibrils
#10
Nava Zaarur, Xiaobin Xu, Patrick Lestienne, Anatoli B Meriin, Mark McComb, Catherine E Costello, Gary P Newnam, Rakhee Ganti, Nina V Romanova, Maruda Shanmugasundaram, Sara T N Silva, Tiago M Bandeiras, Pedro M Matias, Kirill S Lobachev, Igor K Lednev, Yury O Chernoff, Michael Y Sherman
The aggresome is an organelle that recruits aggregated proteins for storage and degradation. We performed an siRNA screen for proteins involved in aggresome formation and identified novel mammalian AAA+ protein disaggregases RuvbL1 and RuvbL2. Depletion of RuvbL1 or RuvbL2 suppressed aggresome formation and caused buildup of multiple cytoplasmic aggregates. Similarly, downregulation of RuvbL orthologs in yeast suppressed the formation of an aggresome-like body and enhanced the aggregate toxicity. In contrast, their overproduction enhanced the resistance to proteotoxic stress independently of chaperone Hsp104...
September 14, 2015: EMBO Journal
https://www.readbyqxmd.com/read/26201077/chromosome-missegregation-associated-with-ruvbl1-deficiency
#11
Christian Gentili, Dennis Castor, Svenja Kaden, David Lauterbach, Mario Gysi, Patrick Steigemann, Daniel W Gerlich, Josef Jiricny, Stefano Ferrari
RUVBL1 (RuvB-like1) and RUVBL2 (RuvB-like 2) are integral components of multisubunit protein complexes involved in processes ranging from cellular metabolism, transcription and chromatin remodeling to DNA repair. Here, we show that although RUVBL1 and RUVBL2 are known to form heterodimeric complexes in which they stabilize each other, the subunits separate during cytokinesis. In anaphase-to-telophase transition, RUVBL1 localizes to structures of the mitotic spindle apparatus, where it partially co-localizes with polo-like kinase 1 (PLK1)...
2015: PloS One
https://www.readbyqxmd.com/read/25806092/methylation-analysis-of-histone-h4k12ac-associated-promoters-in-sperm-of-healthy-donors-and-subfertile-patients
#12
Markus Vieweg, Katerina Dvorakova-Hortova, Barbora Dudkova, Przemyslaw Waliszewski, Marie Otte, Berthold Oels, Amir Hajimohammad, Heiko Turley, Martin Schorsch, Hans-Christian Schuppe, Wolfgang Weidner, Klaus Steger, Agnieszka Paradowska-Dogan
BACKGROUND: Histone to protamine exchange and the hyperacetylation of the remaining histones are hallmarks of spermiogenesis. Acetylation of histone H4 at lysine 12 (H4K12ac) was observed prior to full decondensation of sperm chromatin after fertilization suggesting an important role for the regulation of gene expression in early embryogenesis. Similarly, DNA methylation may contribute to gene silencing of several developmentally important genes. Following the identification of H4K12ac-binding promoters in sperm of fertile and subfertile patients, we aimed to investigate whether the depletion of histone-binding is associated with aberrant DNA methylation in sperm of subfertile men...
2015: Clinical Epigenetics
https://www.readbyqxmd.com/read/25767478/substrate-recognition-and-function-of-the-r2tp-complex-in-response-to-cellular-stress
#13
REVIEW
Patrick von Morgen, Zuzana Hořejší, Libor Macurek
The R2TP complex is a HSP90 co-chaperone, which consists of four subunits: PIH1D1, RPAP3, RUVBL1, and RUVBL2. It is involved in the assembly of large protein or protein-RNA complexes such as RNA polymerase, small nucleolar ribonucleoproteins (snoRNPs), phosphatidylinositol 3 kinase-related kinases (PIKKs), and their complexes. While RPAP3 has a HSP90 binding domain and the RUVBLs comprise ATPase activities important for R2TP functions, PIH1D1 contains a PIH-N domain that specifically recognizes phosphorylated substrates of the R2TP complex...
2015: Frontiers in Genetics
https://www.readbyqxmd.com/read/25453825/ruvs-drive-chromosome-decondensation-after-mitosis
#14
COMMENT
Magdalena Strzelecka, Rebecca Heald
Condensation of chromosomes during mitosis is required for their segregation into daughter cells but must be reversed to allow for postmitotic functions. In this issue of Developmental Cell, Magalska et al. (2014) show that the ATPases RuvBL1/2 drive postmitotic chromatin decondensation, demonstrating that this is an active process.
November 10, 2014: Developmental Cell
https://www.readbyqxmd.com/read/25437307/linkin-a-new-transmembrane-protein-necessary-for-cell-adhesion
#15
Mihoko Kato, Tsui-Fen Chou, Collin Z Yu, John DeModena, Paul W Sternberg
In epithelial collective migration, leader and follower cells migrate while maintaining cell-cell adhesion and tissue polarity. We have identified a conserved protein and interactors required for maintaining cell adhesion during a simple collective migration in the developing C. elegans male gonad. LINKIN is a previously uncharacterized, transmembrane protein conserved throughout Metazoa. We identified seven atypical FG-GAP domains in the extracellular domain, which potentially folds into a β-propeller structure resembling the α-integrin ligand-binding domain...
2014: ELife
https://www.readbyqxmd.com/read/25428364/abundance-of-the-fanconi-anaemia-core-complex-is-regulated-by-the-ruvbl1-and-ruvbl2-aaa-atpases
#16
Eeson Rajendra, Juan I Garaycoechea, Ketan J Patel, Lori A Passmore
Fanconi anaemia (FA) is a genome instability disease caused by defects in the FA DNA repair pathway that senses and repairs damage caused by DNA interstrand crosslinks. At least 8 of the 16 genes found mutated in FA encode proteins that assemble into the FA core complex, a multisubunit monoubiquitin E3 ligase. Here, we show that the RuvBL1 and RuvBL2 AAA+ ATPases co-purify with FA core complex isolated under stringent but native conditions from a vertebrate cell line. Depletion of the RuvBL1-RuvBL2 complex in human cells causes hallmark features of FA including DNA crosslinker sensitivity, chromosomal instability and defective FA pathway activation...
December 16, 2014: Nucleic Acids Research
https://www.readbyqxmd.com/read/25336637/reptin-and-pontin-oligomerization-and-activity-are-modulated-through-histone-h3-n-terminal-tail-interaction
#17
Richard Queval, Christophe Papin, Mathieu Dalvai, Kerstin Bystricky, Odile Humbert
Pontin/RUVBL1 and Reptin/RUVBL2 are DNA-dependent ATPases involved in numerous cellular processes and are essential components of chromatin remodeling complexes and transcription factor assemblies. However, their existence as monomeric and oligomeric forms with differential activity in vivo reflects their versatility. Using a biochemical approach, we have studied the role of the nucleosome core particle and histone N-terminal tail modifications in the assembly and enzymatic activities of Reptin/Pontin. We demonstrate that purified Reptin and Pontin form stable complexes with nucleosomes...
December 5, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24990942/structure-of-yin-yang-1-oligomers-that-cooperate-with-ruvbl1-ruvbl2-atpases
#18
Andrés López-Perrote, Hanan E Alatwi, Eva Torreira, Amani Ismail, Silvia Ayora, Jessica A Downs, Oscar Llorca
Yin Yang 1 (YY1) is a transcription factor regulating proliferation and differentiation and is involved in cancer development. Oligomers of recombinant YY1 have been observed before, but their structure and DNA binding properties are not well understood. Here we find that YY1 assembles several homo-oligomeric species built from the association of a bell-shaped dimer, a process we characterized by electron microscopy. Moreover, we find that YY1 self-association also occurs in vivo using bimolecular fluorescence complementation...
August 15, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24957886/a-multiparametric-serum-marker-panel-as-a-complementary-test-to-mammography-for-the-diagnosis-of-node-negative-early-stage-breast-cancer-and-dcis-in-young-women
#19
Jérôme Lacombe, Alain Mangé, Anne-Claire Bougnoux, Ioannis Prassas, Jérôme Solassol
BACKGROUND: The sensitivity of mammography for the detection of small lesions, including node-negative early-stage (T1N0) primary breast cancer (PBC) and ductal carcinoma in situ (DCIS), is significantly decreased in young patients. From a clinical standpoint, an inconclusive mammogram reflects the inability of clinicians to confidently decide whether patients should be referred for biopsy or for follow-up with repeat imaging. METHODS: Specific ELISAs were developed for a panel of 13 well-recognized breast autoantigens (HSP60, FKBP52, PRDX2, PPIA, MUC1, GAL3, PAK2, P53, CCNB1, PHB2, RACK1, RUVBL1, and HER2)...
September 2014: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/24728183/ruvbl1-directly-binds-actin-filaments-and-induces-formation-of-cell-protrusions-to-promote-pancreatic-cancer-cell-invasion
#20
Keisuke Taniuchi, Mutsuo Furihata, Shinji Iwasaki, Kenjiro Tanaka, Takahiro Shimizu, Motoaki Saito, Toshiji Saibara
We report a novel function of RUVBL1 molecule in pancreatic cancer cells. Previous reports describe that RUVBL1 belongs to the family of AAA+ ATPases that associate with chromatin-remodelling complexes and have important roles in transcriptional regulation, the DNA damage response, telomerase activity and cellular transformation. We show that knockdown of RUVBL1 inhibited the motility and invasiveness of pancreatic cancer cells. RUVBL1 localized in the cytoplasm bound filamentous actin (F-actin) in cell protrusions, and increased concentration of monomeric globular-actin (G-actin) in cell protrusions of migrating pancreatic cancer cells...
June 2014: International Journal of Oncology
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