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https://www.readbyqxmd.com/read/29113992/axonemal-dynein-assembly-requires-the-r2tp-complex-component-pontin
#1
Yuanyuan Li, Lu Zhao, Shiaulou Yuan, Jiefang Zhang, Zhaoxia Sun
Pontin (Ruvbl1) and Reptin (Ruvbl2) are closely related AAA ATPases. They are components of the Ruvbl1-Rvubl2-Tah1-Pih1 (R2TP) complexes that function as co-chaperones for the assembly of multiple macromolecular protein complexes. Here, we show that Pontin is essential for cilia motility in both zebrafish and mouse and that Pontin and Reptin function cooperatively in this process. pontin zebrafish mutants display phenotypes tightly associated with cilia defects and cilia motility is lost in a number of ciliated tissues along with a reduction in the number of both outer and inner dynein arms (ODAs and IDAs)...
November 7, 2017: Development
https://www.readbyqxmd.com/read/28982102/reticulocytes-are-an-enriched-source-of-the-ruvbl1-protein
#2
Beverly W Baron, Rebecca M Baron, Joseph M Baron
No abstract text is available yet for this article.
October 6, 2017: Acta Haematologica
https://www.readbyqxmd.com/read/28884116/the-role-of-pontin-and-reptin-in-cellular-physiology-and-cancer-etiology
#3
REVIEW
Yu-Qian Mao, Walid A Houry
Pontin (RUVBL1, TIP49, TIP49a, Rvb1) and Reptin (RUVBL2, TIP48, TIP49b, Rvb2) are highly conserved ATPases of the AAA+ (ATPases Associated with various cellular Activities) superfamily and are involved in various cellular processes that are important for oncogenesis. First identified as being upregulated in hepatocellular carcinoma and colorectal cancer, their overexpression has since been shown in multiple cancer types such as breast, lung, gastric, esophageal, pancreatic, kidney, bladder as well as lymphatic, and leukemic cancers...
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/28574207/human-dna-helicase-ruvbl1-and-its-chlamydomonas-homologue-crruvbl1-plays-an-important-role-in-ciliogenesis
#4
Damayanti Tammana, Trinadh Venkata Satish Tammana
Several nuclear and nucleic acid-binding proteins were detected in the proteomic analyses of ciliary fractions from various organisms. Yet very little is known about the role of these proteins in ciliogenesis and ciliary signaling. In an attempt to characterize the role of these nuclear proteins, we identified a hypothetical protein from Chlamydomonas reinhardtii, CrRuvBL1, which is homologous to human DNA helicase, HsRuvBL1. CrRuvBL1 localizes to flagella and nucleus in vegetative Chlamydomonas cells. It accumulates in the nucleus specifically during initial stages of flagellar assembly and cell division indicating its role in these processes...
June 2, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/28561026/r2tp-prefoldin-like-component-ruvbl1-ruvbl2-directly-interacts-with-znhit2-to-regulate-assembly-of-u5-small-nuclear-ribonucleoprotein
#5
Philippe Cloutier, Christian Poitras, Mathieu Durand, Omid Hekmat, Émilie Fiola-Masson, Annie Bouchard, Denis Faubert, Benoit Chabot, Benoit Coulombe
The R2TP/Prefoldin-like (R2TP/PFDL) complex has emerged as a cochaperone complex involved in the assembly of a number of critical protein complexes including snoRNPs, nuclear RNA polymerases and PIKK-containing complexes. Here we report on the use of multiple target affinity purification coupled to mass spectrometry to identify two additional complexes that interact with R2TP/PFDL: the TSC1-TSC2 complex and the U5 small nuclear ribonucleoprotein (snRNP). The interaction between R2TP/PFDL and the U5 snRNP is mostly mediated by the previously uncharacterized factor ZNHIT2...
May 31, 2017: Nature Communications
https://www.readbyqxmd.com/read/28453527/altered-nucleocytoplasmic-proteome-and-transcriptome-distributions-in-an-in-vitro-model-of-amyotrophic-lateral-sclerosis
#6
Jee-Eun Kim, Yoon Ho Hong, Jin Young Kim, Gye Sun Jeon, Jung Hee Jung, Byung-Nam Yoon, Sung-Yeon Son, Kwang-Woo Lee, Jong-Il Kim, Jung-Joon Sung
Aberrant nucleocytoplasmic localization of proteins has been implicated in many neurodegenerative diseases. Evidence suggests that cytoplasmic mislocalization of nuclear proteins such as transactive response DNA-binding protein 43 (TDP-43) and fused in sarcoma (FUS) may be associated with neurotoxicity in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration. This study investigated the changes in nucleocytoplasmic distributions of the proteome and transcriptome in an in vitro model of ALS...
2017: PloS One
https://www.readbyqxmd.com/read/28341484/ruvbl1-itfg1-interaction-is-required-for-collective-invasion-in-breast-cancer
#7
Wenjun Fan, Jiajun Xie, Jianglong Xia, Yan Zhang, Mengying Yang, Hefei Wang, Yujia Pan, Mengjuan Zhang, Baochun Han, Baitong Wu, Zhijie Hou, Dapeng Liang, Chunli Wang, Jie Xu, Lijuan Song, Quentin Liu
BACKGROUND: The mechanisms of breast cancer collective invasion are poorly understood limiting the metastasis therapy. The ATPase RUVBL1 is frequently overexpressed in various cancers and plays a crucial role in oncogenic process. We further investigated the role of RUVBL1 in promoting collective invasion and uncovered that targeting RUVBL1 could inhibit metastatic progression. METHODS: The expression levels of RUVBL1 and ITFG1 were examined by Western blot and qRT-PCR...
July 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28238654/prmt5-dependent-methylation-of-the-tip60-coactivator-ruvbl1-is-a-key-regulator-of-homologous-recombination
#8
Thomas L Clarke, Maria Pilar Sanchez-Bailon, Kelly Chiang, John J Reynolds, Joaquin Herrero-Ruiz, Tiago M Bandeiras, Pedro M Matias, Sarah L Maslen, J Mark Skehel, Grant S Stewart, Clare C Davies
Protein post-translation modification plays an important role in regulating DNA repair; however, the role of arginine methylation in this process is poorly understood. Here we identify the arginine methyltransferase PRMT5 as a key regulator of homologous recombination (HR)-mediated double-strand break (DSB) repair, which is mediated through its ability to methylate RUVBL1, a cofactor of the TIP60 complex. We show that PRMT5 targets RUVBL1 for methylation at position R205, which facilitates TIP60-dependent mobilization of 53BP1 from DNA breaks, promoting HR...
March 2, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28122980/suppression-of-type-i-interferon-signaling-by-e1a-via-ruvbl1-pontin
#9
Oladunni Olanubi, Jasmine Rae Frost, Sandi Radko, Peter Pelka
Suppression of interferon signaling is of paramount importance to a virus. Interferon signaling significantly reduces or halts the ability of a virus to replicate; therefore, viruses have evolved sophisticated mechanisms that suppress activation of the interferon pathway or responsiveness of the infected cell to interferon. Adenovirus has multiple modes of inhibiting the cellular response to interferon. Here, we report that E1A, previously shown to regulate interferon signaling in multiple ways, inhibits interferon-stimulated gene expression by modulating RuvBL1 function...
April 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27935167/ck2-phospho-independent-assembly-of-the-tel2-associated-stress-signaling-complexes-in-schizosaccharomyces-pombe
#10
Haruna Inoue, Shizuka Sugimoto, Yumiko Takeshita, Miho Takeuchi, Mitsuko Hatanaka, Koji Nagao, Takeshi Hayashi, Aya Kokubu, Mitsuhiro Yanagida, Junko Kanoh
An evolutionarily conserved protein Tel2 regulates a variety of stress signals. In mammals, TEL2 associates with TTI1 and TTI2 to form the Triple T (TTT: TEL2-TTI1-TTI2) complex as well as with all the phosphatidylinositol 3-kinase-like kinases (PIKKs) and the R2TP (Ruvbl1-Ruvbl2-Tah1-Pih1 in budding yeast)/prefoldin-like complex that associates with HSP90. The phosphorylation of TEL2 by casein kinase 2 (CK2) enables direct binding of PIHD1 (mammalian Pih1) to TEL2 and is important for the stability and the functions of PIKKs...
January 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/27746211/insights-into-specificity-redundancy-and-new-cellular-functions-of-c-ebpa-and-c-ebpb-transcription-factors-through-interactome-network-analysis
#11
COMPARATIVE STUDY
Maurizio Cirilli, Oxana Bereshchenko, Olga Ermakova, Claus Nerlov
BACKGROUND: C/EBPa and C/EBPb are transcription factors with tissue specific expression regulating several important cellular processes. They work by recruiting protein complexes to a common DNA recognition motif and both are able to compensate each other's absence in many cell types, thus showing functional redundancy. They also play distinct roles in specific cellular pathways and their abnormal functioning gives raise to different human pathologies. METHODS: To investigate the molecular basis of C/EBPa and C/EBPb specificity and redundancy we characterized their in vivo protein-protein interaction networks by Tandem Affinity Purification (TAP) and Mass Spectrometry (MS)...
February 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27722820/downregulation-of-ruvbl1-inhibits-proliferation-of-lung-adenocarcinoma-cells-by-g1-s-phase-cell-cycle-arrest-via-multiple-mechanisms
#12
Xiao-Shuai Yuan, Zhi-Tian Wang, Ye-Ji Hu, Fei-Chao Bao, Ping Yuan, Chong Zhang, Jin-Lin Cao, Wang Lv, Jian Hu
Lung cancer remains a leading cause of cancer-related mortality and morbidity worldwide, of which non-small cell lung cancer (NSCLC) accounts for 80 %. RUVBL1 is a highly conserved eukaryotic AAA+ adenosine 5'-triphosphatase (ATPase) that has many functions highly relevant to cancer. We therefore attempted to determine the potential role of RUVBL1 in the biogenesis of lung adenocarcinoma and obtained some interesting results. Our study revealed that RUVBL1 expression was higher in lung adenocarcinoma specimens than in those of adjacent non-tumor tissues and in lung cancer cell lines than in normal lung cell lines...
October 10, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27715451/assembly-and-trafficking-of-box-c-d-and-h-aca-snornps
#13
Séverine Massenet, Edouard Bertrand, Céline Verheggen
Box C/D and box H/ACA snoRNAs are abundant non-coding RNAs that localize in the nucleolus and mostly function as guides for nucleotide modifications. While a large pool of snoRNAs modifies rRNAs, an increasing number of snoRNAs could also potentially target mRNAs. ScaRNAs belong to a family of specific RNAs that localize in Cajal bodies and that are structurally similar to snoRNAs. Most scaRNAs are involved in snRNA modification, while telomerase RNA, which contains H/ACA motifs, functions in telomeric DNA synthesis...
June 3, 2017: RNA Biology
https://www.readbyqxmd.com/read/27664947/ad-e1a-243r-oncoprotein-promotes-association-of-proto-oncogene-product-myc-with-the-nua4-tip60-complex-via-the-e1a-n-terminal-repression-domain
#14
Ling-Jun Zhao, Paul M Loewenstein, Maurice Green
The adenovirus E1A 243R oncoprotein targets TRRAP, a scaffold protein that assembles histone acetyltransferase (HAT) complexes, such as the NuA4/Tip60 complex which mediates transcriptional activity of the proto-oncogene MYC and helps determine the cancer cell phenotype. How E1A transforms cells through TRRAP remains obscure. We performed proteomic analysis with the N-terminal transcriptional repression domain of E1A 243R (E1A 1-80) and showed that E1A 1-80 interacts with TRRAP, p400, and three other members of the NuA4 complex - DMAP1, RUVBL1 and RUVBL2 - not previously shown to associate with E1A 243R...
December 2016: Virology
https://www.readbyqxmd.com/read/27250028/transcriptomic-and-proteomic-analysis-of-mouse-radiation-induced-acute-myeloid-leukaemia-aml
#15
Christophe Badie, Agnieszka Blachowicz, Zarko Barjaktarovic, Rosemary Finnon, Arlette Michaux, Hakan Sarioglu, Natalie Brown, Grainne Manning, M Abderrafi Benotmane, Soile Tapio, Joanna Polanska, Simon D Bouffler
A combined transcriptome and proteome analysis of mouse radiation-induced AMLs using two primary AMLs, cell lines from these primaries, another cell line and its in vivo passage is reported. Compared to haematopoietic progenitor and stem cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all materials. 55 and 3 alterations were detected in the proteomes of the cell lines and primary/in vivo passage material respectively, with one common to all materials. In cell lines, approximately 50% of the transcriptome changes are related to adaptation to cell culture, and in the proteome this proportion was higher...
June 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/27066592/the-relationship-between-ruvbl1-pontin-tip49-nmp238-and-bcl6-in-benign-and-malignant-human-lymphoid-tissues
#16
Beverly W Baron, Rebecca M Baron, Joseph M Baron
The human BCL6 gene, which is involved in the pathogenesis of certain human lymphomas, encodes a transcriptional repressor that is needed for germinal center B cell development and T follicular helper cell differentiation. Our goal was to identify BCL6 target genes using a cell system in which BCL6 repressive effects are inhibited followed by subtractive hybridization, and we detected the RUVBL1 (Pontin, TIP49) gene as a potential target of BCL6 repression. Here we show that the BCL6 protein significantly represses RUVBL1 transcription (6...
July 2016: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/27040446/grifola-frondosa-glycoprotein-gfg-3a-arrests-s-phase-alters-proteome-and-induces-apoptosis-in-human-gastric-cancer-cells
#17
Fengjie Cui, Xinyi Zan, Yunhong Li, Wenjing Sun, Yan Yang, Lifeng Ping
GFG-3a is a novel glycoprotein previously purified from the fermented mycelia of Grifola frondosa with novel sugar compositions and protein sequencing. The present study aims to investigate its effects on the cell cycle, differential proteins expression, and apoptosis of human gastric cancer SGC-7901 cells. Our findings revealed that GFG-3a induced the cell apoptosis and arrested cell cycle at S phase. GFG-3a treatment resulted in the differential expression of 21 proteins in SGC-7901 cells by upregulating 10 proteins including RBBP4 associated with cell cycle arrest and downregulating 11 proteins including RUVBL1, NPM, HSP90AB1, and GRP78 involved in apoptosis and stress response...
2016: Nutrition and Cancer
https://www.readbyqxmd.com/read/26988609/gene-expression-profile-of-the-clinically-aggressive-micropapillary-variant-of-bladder-cancer
#18
Charles Chuanhai Guo, Vipulkumar Dadhania, Li Zhang, Tadeusz Majewski, Jolanta Bondaruk, Maciej Sykulski, Weronika Wronowska, Anna Gambin, Yan Wang, Shizhen Zhang, Enrique Fuentes-Mattei, Ashish Madhav Kamat, Colin Dinney, Arlene Siefker-Radtke, Woonyoung Choi, Keith A Baggerly, David McConkey, John N Weinstein, Bogdan Czerniak
BACKGROUND: Progression of conventional urothelial carcinoma of the bladder to a tumor with unique microscopic features referred to as micropapillary carcinoma is coupled with aggressive clinical behavior signified by a high propensity for metastasis to regional lymph nodes and distant organs resulting in shorter survival. OBJECTIVE: To analyze the expression profile of micropapillary cancer and define its molecular features relevant to clinical behavior. DESIGN, SETTING, AND PARTICIPANTS: We retrospectively identified 43 patients with micropapillary bladder cancers and a reference set of 89 patients with conventional urothelial carcinomas and performed whole-genome expression messenger RNA profiling...
October 2016: European Urology
https://www.readbyqxmd.com/read/26711270/a-novel-interaction-of-ecdysoneless-ecd-protein-with-r2tp-complex-component-ruvbl1-is-required-for-the-functional-role-of-ecd-in-cell-cycle-progression
#19
Riyaz A Mir, Aditya Bele, Sameer Mirza, Shashank Srivastava, Appolinaire A Olou, Shalis A Ammons, Jun Hyun Kim, Channabasavaiah B Gurumurthy, Fang Qiu, Hamid Band, Vimla Band
Ecdysoneless (ECD) is an evolutionarily conserved protein whose germ line deletion is embryonic lethal. Deletion of Ecd in cells causes cell cycle arrest, which is rescued by exogenous ECD, demonstrating a requirement of ECD for normal mammalian cell cycle progression. However, the exact mechanism by which ECD regulates cell cycle is unknown. Here, we demonstrate that ECD protein levels and subcellular localization are invariant during cell cycle progression, suggesting a potential role of posttranslational modifications or protein-protein interactions...
December 28, 2015: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/26303906/ruvbl1-and-ruvbl2-enhance-aggresome-formation-and-disaggregate-amyloid-fibrils
#20
Nava Zaarur, Xiaobin Xu, Patrick Lestienne, Anatoli B Meriin, Mark McComb, Catherine E Costello, Gary P Newnam, Rakhee Ganti, Nina V Romanova, Maruda Shanmugasundaram, Sara T N Silva, Tiago M Bandeiras, Pedro M Matias, Kirill S Lobachev, Igor K Lednev, Yury O Chernoff, Michael Y Sherman
The aggresome is an organelle that recruits aggregated proteins for storage and degradation. We performed an siRNA screen for proteins involved in aggresome formation and identified novel mammalian AAA+ protein disaggregases RuvbL1 and RuvbL2. Depletion of RuvbL1 or RuvbL2 suppressed aggresome formation and caused buildup of multiple cytoplasmic aggregates. Similarly, downregulation of RuvbL orthologs in yeast suppressed the formation of an aggresome-like body and enhanced the aggregate toxicity. In contrast, their overproduction enhanced the resistance to proteotoxic stress independently of chaperone Hsp104...
September 14, 2015: EMBO Journal
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