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https://www.readbyqxmd.com/read/28088353/expansion-and-differentiation-of-igm-b-cells-in-the-rainbow-trout-peritoneal-cavity-in-response-to-different-antigens
#1
Rosario Castro, Beatriz Abós, Lucia González, Aitor G Granja, Carolina Tafalla
To date, intraperitoneal (i.p.) injection seems to be the most effective vaccination route in aquaculture, as many i.p. administered fish vaccines are capable of conferring strong and long-lasting immune responses. Despite this, how peritoneal leukocytes are regulated upon antigen encounter has only been scarcely studied in fish. Although, in the past, myeloid cells were thought to be the main responders to peritoneal inflammation, a recent study revealed that IgM(+) B cells are one of the main cell types in the teleost peritoneal cavity in response to pathogenic bacteria...
January 11, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28088341/evaluation-of-an-in-house-indirect-elisa-for-detection-of-antibody-against-haemorrhagic-septicemia-in-asian-elephants
#2
Pallop Tankaew, Thawatchai Singh-La, Chatchote Titaram, Veerasak Punyapornwittaya, Preeyanat Vongchan, Takuo Sawada, Nattawooti Sthitmatee
Pasteurella multocida causes haemorrhagic septicemia in livestock and wild animals, including elephants. The disease has been reported in Asian elephants in India and Sri Lanka, but to date there have been no reported cases in Thailand. ELISA or indirect hemagglutination assays (IHA) have been demonstrated to be able to detect the antibody against the disease in cattle, but no data are available for elephants. The present study reports a novel in-house indirect ELISA for antibody detection of haemorrhagic septicemia in Asian elephants, and evaluates the sensitivity and specificity of the method using a Bayesian approach...
January 11, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28081204/deletion-of-ssna-attenuates-the-pathogenicity-of-streptococcus-suis-and-confers-protection-against-serovar-2-strain-challenge
#3
Miao Li, Ru-Jian Cai, Chun-Ling Li, Shuai Song, Yan Li, Zhi-Yong Jiang, Dong-Xia Yang
Streptococcus suis serotype 2 (SS2) is a major porcine and human pathogen which causes arthritis, meningitis, and septicemia. Streptococcus suis nuclease A (SsnA) is a recently discovered deoxyribonuclease (DNase), which has been demonstrated to contribute to escape killing in neutrophil extracellular traps (NETs). To further determine the effects of ssnA on virulence, the ssnA deletion mutant (ΔssnA) and its complemented strain (C-ΔssnA) were constructed. The ability of ΔssnA mutant to interact with human laryngeal epithelial cell (Hep-2) was evaluated and it exhibited dramatically decreased ability to adhere to and invade Hep-2 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28078861/interleukin-6-and-interleukin-8-in-diagnosing-neonatal-septicemia
#4
Y Q Wu, J Shen, Q L Zhou, H W Zhao, L R Liu, X Liu
Neonatal septicemia (NS) is a common cause of death of newborn infants, hence early diagnosis and treatment are of the utmost importance. However, lack of specific clinical symptoms and late detection delay a correct diagnosis. It is therefore of great importance to establish auxiliary indexes for the early diagnosis of NS. To evaluate the value of interleukin (IL-6 and IL-8) in the diagnosis of NS, a prospective study was carried out. Seventy-five newborns who developed septicemia and received treatment in our hospital from January 2013 to December 2014 were selected as research subjects; also, 50 healthy newborns were set as a control group...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28063953/pathological-analysis-detection-of-antigens-fasl-expression-analysis-and-leucocytes-survival-analysis-in-tilapia-oreochromis-niloticus-after-infection-with-green-fluorescent-protein-labeled-streptococcus-agalactiae
#5
Jingyuan Wang, Jinying Wu, Liyuan Yi, Zengxin Hou, Wensheng Li
The pathogenesis of Streptococcus agalactiae infection in tilapia has not been fully described. To understand this, we investigated the clinic-pathological features of acute experimental septicemia in tilapia (Oreochromis niloticus) after receiving an intra-peritoneal injection with S. agalactiae THN-1901GFP. Immunohistochemistry and sections of pathological tissues were used to estimate the level of damage in the head-kidney, liver, spleen and trunk-kidney. The expression of FasL was analyzed by western blotting in these samples based on their damage levels...
January 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28061775/immunization-with-streptococcus-suis-bacterin-plus-recombinant-sao-protein-in-sows-conveys-passive-immunity-to-their-piglets
#6
Kai-Jen Hsueh, Li-Ting Cheng, Jai-Wei Lee, Yao-Chi Chung, Wen-Bin Chung, Chun-Yen Chu
BACKGROUND: Streptococcus suis (S. suis) causes arthritis, meningitis, septicemia, and sudden death in pigs and is also an zoonotic agent for humans. The present study demonstrated that immunization with recombinant Sao-L (surface antigen one-L, rSao-L) protein from a strain of S. suis serotype 2 in pigs was able to increase cross-serotype protection against S. suis serotype 1 and 2 challenge. Since weaning piglets are more susceptible to S. suis infections due to the stresses associated with weaning, prepartum immunization in sows may convey passive immunity to piglets and provide protection...
January 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28057987/factors-predicting-infectious-complications-following-percutaneous-nephrolithotomy
#7
Kuldeep Sharma, Satya Narayan Sankhwar, Apul Goel, Vishwajeet Singh, Pradeep Sharma, Yogesh Garg
OBJECTIVE: To determine the predictors of infectious complications following percutaneous nephrolithotomy (PCNL) in a prospective study. MATERIALS AND METHODS: A total of 332 patients with renal or upper ureteric calculi who underwent PCNL between January 2013 and June 2014 were included in the study. Infectious complications included febrile urinary tract infection and septicemia. The patients were divided into Group A and B depending on whether they developed or did not develop infectious complications...
October 2016: Urology Annals
https://www.readbyqxmd.com/read/28057750/whole-genome-sequences-and-classification-of-streptococcus-agalactiae-strains-isolated-from-laboratory-reared-long-evans-rats-rattus-norvegicus
#8
C Bodi Winn, J Dzink-Fox, Y Feng, Z Shen, V Bakthavatchalu, J G Fox
In collaboration with the CDC's Streptococcus Laboratory, we report here the whole-genome sequences of seven Streptococcus agalactiae bacteria isolated from laboratory-reared Long-Evans rats. Four of the S. agalactiae isolates were associated with morbidity accompanied by endocarditis, metritis, and fatal septicemia, providing an opportunity for comparative genomic analysis of this opportunistic pathogen.
January 5, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28046014/pseudomonas-aeruginosa-sera-gene-is-required-for-bacterial-translocation-through-caco-2-cell-monolayers
#9
Masashi Yasuda, Syouya Nagata, Satoshi Yamane, Chinami Kunikata, Yutaka Kida, Koichi Kuwano, Chigusa Suezawa, Jun Okuda
To specify critical factors responsible for Pseudomonas aeruginosa penetration through the Caco-2 cell epithelial barrier, we analyzed transposon insertion mutants that demonstrated a dramatic reduction in penetration activity relative to P. aeruginosa PAO1 strain. From these strains, mutations could be grouped into five classes, specifically flagellin-associated genes, pili-associated genes, heat-shock protein genes, genes related to the glycolytic pathway, and biosynthesis-related genes. Of these mutants, we here focused on the serA mutant, as the association between this gene and penetration activity is yet unknown...
2017: PloS One
https://www.readbyqxmd.com/read/28042214/study-of-invasive-pneumococcal-infection-in-adults-with-reference-to-penicillin-resistance
#10
Vrishali Avinash Muley, Dnyaneshwari Purushottam Ghadage, Gauri Eknath Yadav, Arvind Vamanrao Bhore
BACKGROUND: Invasive pneumococcal infections often prove rapidly fatal, even where good medical treatment is readily available. In developed countries, up to 20% of people who contract pneumococcal meningitis die; however, in developing world, mortality is closer to 50%, even among hospitalized patients. The World Health Organization estimated 600,000-800,000 adult deaths each year from pneumococcal pneumonia, meningitis, and sepsis. AIMS: This study aims to estimate isolation rate of invasive pneumococcal infection in adults, to determine the antimicrobial susceptibility profile of Streptococcus pneumoniae isolates and to study the associated risk factors...
January 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28036314/etiology-of-early-onset-septicemia-among-neonates-at-the-university-college-hospital-ibadan-nigeria
#11
Abimbola Ellen Akindolire, Olukemi Tongo, Hannah Dada-Adegbola, Olusegun Akinyinka
INTRODUCTION: Neonatal septicemia remains a major cause of newborn deaths in developing countries. Its burden is further compounded by the emergence of multidrug-resistant pathogens, which is related to a lack of antibiotic protocols resulting in unrestricted use of antibiotics. The absence of reliable antibiotic sensitivity testing makes the formulation of antibiotic guidelines and judicious use of antibiotics difficult. This study sought to identify the current bacterial agents associated with early onset septicemia (EOS; age <72 hours) and their antibiotic susceptibility patterns among neonates at the University College Hospital, Ibadan, Nigeria...
December 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28033241/financial-resource-utilization-and-mortality-impacts-of-teaching-hospital-status-on-pediatric-patients-admitted-for-sepsis
#12
Benson S Hsu, Benjamin D Meyer, Saquib A Lakhani
BACKGROUND: With the changing healthcare landscape in the United States, teaching hospitals face increasing pressure to provide medical education as well as cost effective care. Our study investigated the financial, resource utilization, and mortality impact of teaching hospital status on pediatric patients admitted with sepsis. METHODS: We conducted a retrospective, weighted statistical analysis of hospitalized children with the diagnosis of sepsis. The Agency for Healthcare Research and Quality (AHRQ) 2009 Kids' Inpatient Database (KID) provided the data for analysis...
December 28, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28032191/chitin-degradation-and-utilization-by-virulent-aeromonas-hydrophila-strain-ml10-51k
#13
Dunhua Zhang, De-Hai Xu, Junqiang Qiu, Cody R Rasmussen-Ivey, Mark R Liles, Benjamin H Beck
Virulent Aeromonas hydrophila (vAh) is one of the most important bacterial pathogens that causes persistent outbreaks of motile Aeromonas septicemia in warm-water fishes. The survivability of this pathogen in aquatic environments is of great concern. The aim of this study was to determine the capability of the vAh strain ML10-51K to degrade and utilize chitin. Genome-wide analysis revealed that ML10-51K encodes a suite of proteins for chitin metabolism. Assays in vitro showed that four chitinases, one chitobiase and one chitin-binding protein were secreted extracellularly and participated in chitin degradation...
December 28, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/28009331/a-study-of-acute-kidney-injury-in%C3%A2-hospitalized-preterm-neonates-in-nicu
#14
N Nagaraj, P K Berwal, A Srinivas, A Berwal
OBJECTIVES: This study was carried out to determine the incidence, clinical features, etiology and outcome of functional and intrinsic acute kidney injury (AKI) in preterm neonates. METHODS: This is a prospective observational study on premature infants admitted to the neonatal intensive care unit (NICU) over an eight month period. All biochemical parameters of renal function tests were monitored and statically analyzed. RESULTS: The study included 450 infants; of them 300 were inborn and 150 infants were outborn and transported to the NICU...
2016: Journal of Neonatal-perinatal Medicine
https://www.readbyqxmd.com/read/28007485/viral-hemorrhagic-septicemia-virus-vhsv-infection-mediated-sequential-changes-in-micrornas-profile-of-epithelioma-papulosum-cyprini-epc-cells
#15
Abdellaoui Najib, Min Sun Kim, Ki Hong Kim
MicroRNAs are small non-coding RNAs and are involved in the regulation of wide biological processes. Viral hemorrhagic septicemia virus (VHSV) is the causative agent of viral hemorrhagic septicemia (VHS) disease causing a heavy loss in aquaculture farms. In this study, we tried to explore the effect of VHSV infection on microRNAs profile of Epithelioma papulosum cyprini (EPC) cells at different points of time (0, 3, 12, 24, and 48 h post infection). A total of 355 conserved microRNAs and 3 novel microRNAs were identified, and among them, 103 microRNAs were differentially expressed...
December 20, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28005211/restricted-replication-of-viral-hemorrhagic-septicemia-virus-vhsv-in-a-birnavirus-carrier-cell-culture
#16
Ricardo Parreño, Lucía Almagro, Melissa Belló-Pérez, Regla M Medina-Gali, Amparo Estepa, Luis Perez
Viral hemorrhagic septicemia virus (VHSV) and infectious pancreatic necrosis virus (IPNV) are economically important pathogens of the salmonid aquaculture industry. In previous work we demonstrated that a cell line persistently infected with IPNV (EPC(IPNV)) exhibited antiviral activity against superinfection with the heterologous virus VHSV. This work extends our study by analyzing the replication of VHSV in the IPNV-persistently infected cells. At early and late stages of infection VHSV RNA synthesis, as well as VHSV-induced syncytia formation, were examined in EPC(IPNV) cultures...
December 22, 2016: Archives of Virology
https://www.readbyqxmd.com/read/28001143/vital-signs-and-their-cross-correlation-in-sepsis-and-nec-a-study-of-1-065-very-low-birth-weight-infants-in-two-nicus
#17
Karen D Fairchild, Douglas E Lake, John Kattwinkel, J Randall Moorman, David A Bateman, Philip G Grieve, Joseph R Isler, Rakesh Sahni
BACKGROUND: Subtle changes in vital signs and their interactions occur in preterm infants prior to overt deterioration from late-onset septicemia (LOS) or necrotizing enterocolitis (NEC). Optimizing predictive algorithms may lead to earlier treatment. METHODS: For 1,065 very-low-birth-weight (VLBW) infants in two neonatal intensive care units (NICUs), mean, SD, and cross-correlation of respiratory rate, heart rate (HR), and oxygen saturation (SpO2) were analyzed hourly (131 infant-years' data)...
December 21, 2016: Pediatric Research
https://www.readbyqxmd.com/read/27998866/-nosocomial-septicemia-in-the-early-stage-after-stem-cell-transplantation-in-children-with-major-%C3%AE-thalassemia
#18
Yan-Jun Xu, Chun-Fu Li, Yue-Lin He, Xiao-Qin Feng, Fu-Yu Pei, Xue-Dong Wu
OBJECTIVE: To explore the clinical characteristics of nosocomial septicemia in the early stage after hematopoietic stem cell transplantation (HSCT) in children with major β-thalassemia. METHODS: The clinical data were retrospectively analyzed of 55 consecutive children with major β-thalassemia who developed septicemia early after HSCT between January, 2011 and June, 2016. RESULTS: Among the total of 416 consecutive children with major β-thalassemia undergoing allogeneic HSCT, the incidence of nosocomial infection early after transplantation was 77...
December 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27998162/antibiotic-sensitivity-pattern-of-bacterial-isolates-of-neonatal-septicemia-in-peshawar-pakistan
#19
Obaid Ullah, Aftab Khan, Arif Ambreen, Israr Ahmad, Tasleem Akhtar, Amin Jan Gandapor, Arif Mehmood Khan
BACKGROUND: Septicemia plays an important role in neonatal morbidity and mortality, especially in developing countries. OBJECTIVE: To investigate the bacterial pathogens causing neonatal sepsis and their antibiotic susceptibility profile. METHODOLOGY: A total of 2,685 neonates aged 0-28 days were included in the study. Blood from each neonate was cultured and isolates were identified using standard biochemical tests. Antibiotic sensitivity pattern was analyzed using modified Kirby-Bauer disc diffusion method...
December 2016: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/27995553/role-of-neutrophil-to-lymphocyte-and-monocyte-to-lymphocyte-ratios-in-the-diagnosis-of-bacterial-infection-in-patients-with-fever
#20
Are Naess, Siri Saervold Nilssen, Reidun Mo, Geir Egil Eide, Haakon Sjursen
PURPOSE: To study the role of the neutrophil:lymphocyte ratio (NLR) and monocyte:lymphocyte ratio (MLR) in discriminating between different patient groups hospitalized for fever due to infection and those without infection. METHODS: For 299 patients admitted to hospital for fever with unknown cause, a number of characteristics including NLR and MLR were recorded. These characteristics were used in a multiple multinomial regression analysis to estimate the probability of a final diagnostic group of bacterial, viral, clinically confirmed, or no infection...
December 19, 2016: Infection
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