Read by QxMD icon Read


Eun Jeong Jang, Sang Chan Kim, Ju-Hee Lee, Jong Rok Lee, Il Kon Kim, Su Youn Baek, Young Woo Kim
BACKGROUND: Laminaria japonica has frequently been used as a food supplement and drug in traditional oriental medicine. Among the major active constituents responsible for the bioactivities of L. japonica, fucoxanthin (FX) has been considered as a potential antioxidant. This study was conducted to examine the effects of L. japonica extract (LJE) or FX against oxidative stress on hepatocytes and to elucidate the overall their cellular mechanisms of the effects. METHODS: We constructed an in vitro model with the treatment of arachidonic acid (AA) + iron in HepG2 cells to stimulate the oxidative damage...
March 20, 2018: BMC Complementary and Alternative Medicine
Tânia Patrícia Almeida, Joana Ferreira, Ariane Vettorazzi, Amaia Azqueta, Eduardo Rocha, Alice Abreu Ramos
In the present study, we evaluate the in vitro cytotoxicity of fucoxanthin (Fx) on two human leukemia cell lines, K562 and TK6, alone and in combination with the conventional anticancer drugs imatinib (Imat) and doxorubicin (Dox). For the purpose, we assessed the cytotoxic and proliferation effects by cell count, induction of DNA damage by comet assay, and cell death by nuclear condensation, annexin V staining, coupled with propidium iodide uptake, and protein expression of Bax, caspase-3, and Bcl-2 (western blot)...
February 16, 2018: Environmental Toxicology and Pharmacology
Alexey Moskalev, Mikhail Shaposhnikov, Nadezhda Zemskaya, Alexey Belyi, Eugenia Dobrovolskaya, Anna Patova, Zulfiya Guvatova, Elena Lukyanova, Anastasiya Snezhkina, Anna Kudryavtseva
BACKGROUND: We have previously showed that the carotenoid fucoxanthin can increase the lifespan in Drosophila melanogaster and Caenorhabditis elegans. However, the molecular mechanisms of the geroprotective effect of fucoxanthin have not been studied so far. RESULTS: Here, we studied the effects of fucoxanthin on the Drosophila aging process at the molecular and the whole organism levels. At the organismal level, fucoxanthin increased the median lifespan and had a positive effect on fecundity, fertility, intestinal barrier function, and nighttime sleep...
February 9, 2018: BMC Genomics
Robert G West, David Bína, Marcel Fuciman, Valentyna Kuznetsova, Radek Litvín, Tomáš Polívka
We have applied femtosecond transient absorption spectroscopy in pump-probe and pump-dump-probe regimes to study energy transfer between fucoxanthin and Chl a in fucoxanthin-Chl a complex from the pennate diatom Phaeodactylum tricornutum. Experiments were carried out at room temperature and 77 K to reveal temperature dependence of energy transfer. At both temperatures, the ultrafast (<100 fs) energy transfer channel from the fucoxanthin S2 state is active and is complemented by the second pathway via the combined S1 /ICT state...
February 27, 2018: Biochimica et Biophysica Acta
Peipei Sun, Ka-Wing Cheng, Yongjin He, Bin Liu, Xuemei Mao, Feng Chen
The formation and accumulation of advanced glycation endproducts (AGEs) have been implicated in the pathogenesis of many chronic diseases, such as aging, Alzheimer' s disease, diabetes and diabetic complications. The present study was aimed to investigate the inhibitory effects of the extracts from nine microalgae on the formation of AGEs by using in vitro models and identify key antiglycation constituents of the microalgae. A BSA-glucose model with simulated physiological conditions was used to evaluate the inhibitory effect on total AGE formation...
February 23, 2018: Food & Function
Hristina Staleva-Musto, Valentyna Kuznetsova, Robert G West, Gurkan Kesan, Babak Minofar, Marcel Fuciman, David Bina, Radek Litvin, Tomas Polivka
We used ultrafast transient absorption spectroscopy to study excited-state dynamics of the keto-carotenoid fucoxanthin (Fx) and its two derivatives: 19'-butanoyloxyfucoxanthin (bFx) and 19'-hexanoyloxyfucoxanthin (hFx). These derivatives occur in some light-harvesting systems of photosynthetic microorganisms and their presence is typically related to stress conditions. Even though the hexanoyl (butanoyl) moiety is not a part of the conjugated system of bFx and hFx, their absorption spectra in polar solvents exhibit more pronounced vibrational bands of the S2 state than for Fx...
February 22, 2018: Journal of Physical Chemistry. B
Li-Juan Wang, Yong Fan, Ronald L Parsons, Guang-Rong Hu, Pei-Yu Zhang, Fu-Li Li
Fucoxanthin is a natural pigment found in microalgae, especially diatoms and Chrysophyta. Recently, it has been shown to have anti-inflammatory, anti-tumor, and anti-obesityactivity in humans. Phaeodactylum tricornutum is a diatom with high economic potential due to its high content of fucoxanthin and eicosapentaenoic acid. In order to improve fucoxanthin production, physical and chemical mutagenesis could be applied to generate mutants. An accurate and rapid method to assess the fucoxanthin content is a prerequisite for a high-throughput screen of mutants...
January 22, 2018: Marine Drugs
Ye Jin, Shuang Qiu, Na Shao, Jianhua Zheng
BACKGROUND Fucoxanthin is a carotenoid present in the chloroplasts of brown seaweeds. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a cytokine that selectively induces apoptosis in many tumor cells and is an attractive candidate for antitumor therapies. MATERIAL AND METHODS After human cervical cancer cell lines HeLa, SiHa, and CaSki were treated with fucoxanthin or TRAIL. Cell viability was determined by 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2-tetrazolium 5-carboxanilide (XTT) method...
January 1, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Tjaart P J Krüger, Pavel Malý, Maxime T A Alexandre, Tomáš Mančal, Claudia Büchel, Rienk van Grondelle
Strong excitonic interactions are a key design strategy in photosynthetic light harvesting, expanding the spectral cross-section for light absorption and creating considerably faster and more robust excitation energy transfer. These molecular excitons are a direct result of exceptionally densely packed pigments in photosynthetic proteins. The main light-harvesting complexes of diatoms, known as fucoxanthin-chlorophyll proteins (FCPs), are an exception, displaying surprisingly weak excitonic coupling between their chlorophyll (Chl) a 's, despite a high pigment density...
December 26, 2017: Proceedings of the National Academy of Sciences of the United States of America
Jie Zeng, Yiping Zhang, Jinpeng Ruan, Zhenggang Yang, Chonggang Wang, Zhuan Hong, Zhenghong Zuo
Tributyltin (TBT) is a biocide extremely toxic to a wide range of organisms, which has been used for decades for industrial purposes. Fucoxanthin is a natural carotenoid that is isolated from seaweed, and fucoxanthinol is a major primary metabolite of fucoxanthin. Although fucoxanthin and fucoxanthinol have been reported to possess anti-oxidant activities in vitro, little is known as to whether they protect against TBT-induced oxidative stress in cultured cells. In the present study, the protective effect of fucoxanthin and fucoxanthinol against oxidative stress induced by TBT was investigated...
February 2018: Environmental Science and Pollution Research International
Candida J Rebello, Frank L Greenway, William D Johnson, David Ribnicky, Alexander Poulev, Krisztian Stadler, Ann A Coulter
Rodent studies suggest that the antiobesity effects of fucoxanthin relate to activation of brown fat and conversion of white adipocytes to the brown phenotype. To evaluate the browning effect in human adipocytes, we investigated the genes involved in browning and measured the oxygen consumption rate (OCR). Data were analyzed by one way ANOVA. Relative to control, fucoxanthinol (1 μM, 0.1 μM, 0.01 μM, 1 nM, 0.1 nM), the metabolite present in human plasma, stimulated lipolysis acutely (mean ± SEM: 4.2 ± 0...
December 20, 2017: Journal of Agricultural and Food Chemistry
Christian Galasso, Cinzia Corinaldesi, Clementina Sansone
As is the case for terrestrial organisms, carotenoids represent the most common group of pigments in marine environments. They are generally biosynthesized by all autotrophic marine organisms, such as bacteria and archaea, algae and fungi. Some heterotrophic organisms also contain carotenoids probably accumulated from food or partly modified through metabolic reactions. These natural pigments are divided into two chemical classes: carotenes (such as lycopene and α- and β-carotene) that are composed of hydrogen and carbon; xanthophylls (such as astaxanthin, fucoxanthin and lutein), which are constituted by hydrogen, carbon and oxygen...
November 23, 2017: Antioxidants (Basel, Switzerland)
Kyungsu Kang, Lei Peng, Yu-Jin Jung, Joo Yeon Kim, Eun Ha Lee, Hee Ju Lee, Sang Min Kim, Sang Hyun Sung, Cheol-Ho Pan, Yongsoo Choi
OBJECTIVES: To develop a high-throughput screening system to measure the conversion of testosterone to dihydrotestosterone (DHT) in cultured human prostate cancer cells using turbulent flow chromatography liquid chromatography-triple quadrupole mass spectrometry (TFC-LC-TQMS). RESULTS: After optimizing the cell reaction system, this method demonstrated a screening capability of 103 samples, including 78 single compounds and 25 extracts, in less than 12 h without manual sample preparation...
November 21, 2017: Biotechnology Letters
Snezana Agatonovic-Kustrin, David W Morton
High-Performance Thin-layer chromatography (HPTLC) combined with DPPH free radical method and α-amylase bioassay was used to compare antioxidant and antidiabetic activities in ethanol and ethyl acetate extracts from 10 marine macroalgae species (3 Chlorophyta, 4 Phaeophyta and 3 Rhodophyta) from Blue Lagoon beach (Malaysia). Samples were also evaluated for their phenolic and stigmasterol content. On average, higher antioxidant activity was observed in the ethyl acetate extracts (55.1mg/100g gallic acid equivalents (GAE) compared to 35...
December 29, 2017: Journal of Chromatography. A
Yi-Hsien Chang, Ya-Ling Chen, Wen-Chung Huang, Chian-Jiun Liou
The fucoxanthin, isolated from brown algae, was reported to have multiple biological functions to anti-inflammation, anti-tumor, and ameliorated obesity in mice. In this study we investigated whether fucoxanthin could inhibit lipids accumulation in FL83B hepatocytes. FL83B cells were induced as fatty liver cell model by 0.5 mM oleic acid for 48 h, and treated with various concentration of fucoxanthin for 24 h. The results demonstrated that fucoxanthin significantly suppressed lipid accumulation and decreased lipid peroxidation in hepatocytes...
November 4, 2017: Biochemical and Biophysical Research Communications
Rui-Xue Yu, Rui-Tao Yu, Zhong Liu
Fucoxanthin is a natural carotenoid that had never been previously demonstrated to have anti-tumor effect on human gastric adenocarcinoma SGC-7901 or BGC-823 cells. Here it was found to inhibit proliferation and induce apoptosis through JAK/STAT signal pathway in these cells; the mechanism by which this occurred was investigated. We find that fucoxanthin significantly increased the number of apoptotic cells by propidium iodide (PI) dye staining and flow cytometry. Fucoxanthin (50 or 75 μM) induced SGC-7901 cells cycle arrest at S phase, while BGC-823 cells arrest at G2/M phase...
November 6, 2017: Human Cell
Liang Shen, Yongqin Liu, Ninglian Wang, Nianzhi Jiao, Baiqing Xu, Xiaobo Liu
It has been suggested that the cryosphere is a new biome uniquely dominated by microorganisms, although the ecological characteristics of these cold-adapted bacteria are not well understood. We investigated the vertical variation with depth of the proportion of pigmented bacteria recovered from an ice core drilled in the Yuzhufeng Glacier, Tibetan Plateau. A total of 25,449 colonies were obtained from 1250 ice core sections. Colonies grew on only one-third of the inoculated Petri dishes, indicating that although the ice core harbored abundant culturable bacteria, bacteria could not be isolated from every section...
October 25, 2017: Extremophiles: Life Under Extreme Conditions
Vesa Havurinne, Esa Tyystjärvi
Light-dependent electron transfer is necessary for photosynthesis, but light also damages PSII. Light-induced damage to PSII is called photoinhibition, and the damaging reactions of photoinhibition are still under debate. Diatoms possess an exotic combination of light-harvesting pigments, Chls a/c and fucoxanthin, making them an interesting platform for studying the photoreceptors of photoinhibition. We first confirmed the direct proportionality of photoinhibition to the photon flux density of incident light in the diatom Phaeodactylum tricornutum...
December 1, 2017: Plant & Cell Physiology
Maxime C Bridoux, Monika Sobiechowska, Alicia Pérez-Fuentetaja, Katherine T Alben
Carotenyl fatty acid esters (carotenyl-FAEs) were extracted in acetone from freeze-dried Dreissena bugensis (Lakes Erie and Ontario) and hydrolyzed to identify the carotenoid precursors. Analysis by liquid chromatography (LC) with photodiode array (PDA) and atmospheric pressure chemical ionization-ion trap mass spectrometry (APCIitMS) revealed the major hydrolysis products: fucoxanthinol (FOH) from fucoxanthin (FX, diatoms); mactraxanthin (MX) from violaxanthin (VX, chlorophytes); 4-fold higher levels of an unknown, tentatively identified as an adduct of two closely eluting C27H46O3 and C27H48O3 steryl triols...
October 13, 2017: Analytical and Bioanalytical Chemistry
Tianning Zhang, Cheng Liu, Wenjing Dong, Wenda Wang, Yan Sun, Xin Chen, Chunhong Yang, Ning Dai
Open-circuit photovoltage (Voc ) is among the critical parameters for achieving an efficient light-to-charge conversion in existing solar photovoltaic devices. Natural photosynthesis exploits light-harvesting chlorophyll (Chl) protein complexes to transfer sunlight energy efficiently. We describe the exploitation of photosynthetic fucoxanthin-chlorophyll protein (FCP) complexes for realizing photoelectrochemical cells with a high Voc . An antenna-dependent photocurrent response and a Voc up to 0.72 V are observed and demonstrated in the bio-photovoltaic devices fabricated with photosynthetic FCP complexes and TiO2 nanostructures...
December 5, 2017: Chemistry, An Asian Journal
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"