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https://www.readbyqxmd.com/read/29779031/a-novel-ex-vivo-mouse-mesometrium-culture-model-for-investigating-angiogenesis-in-microvascular-networks
#1
Ariana D Suarez-Martinez, Susanne Bierschenk, Katie Huang, Dana Kaplan, Carolyn L Bayer, Stryder M Meadows, Markus Sperandio, Walter L Murfee
BACKGROUND: The development of models that incorporate intact microvascular networks enables the investigation of multicellular dynamics during angiogenesis. Our laboratory introduced the rat mesentery culture model as such a tool, which would be enhanced with mouse tissue. Since mouse mesentery is avascular, an alternative is mouse mesometrium, the connective tissue of uterine horns. The study's objective was to demonstrate that mouse mesometrium contains microvascular networks that can be cultured to investigate multicellular dynamics during angiogenesis...
May 18, 2018: Journal of Vascular Research
https://www.readbyqxmd.com/read/29748088/innate-immune-cells-in-the-placental-bed-in-healthy-pregnancy-and-preeclampsia
#2
Marijke M Faas, Paul De Vos
Immune cells in the placental bed are important for adequate development of the placental bed. The most prominent immune cells in the placental bed early in pregnancy are uterine natural killer cells (uNK) cells and macrophages. Also dendritic cells and mast cells can be found in the early placental bed. These cells not only have an immune regulatory function, but are also involved in the regulation of trophoblast invasion, angiogenesis and spiral artery remodeling. In preeclampsia, one of the major complications of pregnancy, decreased trophoblast invasion and spiral artery remodeling has been found...
April 25, 2018: Placenta
https://www.readbyqxmd.com/read/29701828/sex-bias-of-the-birth-litter-affects-surge-but-not-tonic-lh-secretion-in-gilts
#3
J Seyfang, R N Kirkwood, A J Tilbrook, C R Ralph
The physiology and behaviour of gilts that develop in a male biased litter can differ from gilts that develop in a female biased litter. We hypothesized that gilts from male biased litters will have a delayed and attenuated LH surge, and reduced LH pulse frequency and amplitude compared to gilts from female biased litters. Gilts were selected at birth from male biased (> 60 % males n = 10) or female biased (> 60 % females n = 9) litters. From 18 weeks of age, detection of puberty using daily boar contact began and their subsequent estrous periods were synchronized with oral progestogen (altrenogest)...
April 26, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29694619/functional-magnetic-resonance-imaging-for-clinical-evaluation-of-uterine-contractility
#4
Vinicius Adami Vayego Fornazari, Stela Adami Vayego, Denis Szejnfeld, Jacob Szejnfeld, Suzan Menasce Goldman
Uterine contractility out of the gestational phase, during the menstrual cycle and the habitual functional variations of the organ, this is one of the responsible mechanisms for reproduction and fertility, due to its direct action in the mechanisms conducting the spermatozoa to the ovule and in the decidual implantation. Pathologies such as uterine leiomyoma, endometriosis, adenomyosis, polycystic ovarian syndrome, as well as the use of intrauterine devices and oral contraceptives, may alter a functionality of uterine contractility...
2018: Einstein
https://www.readbyqxmd.com/read/29675490/evaluation-of-changes-in-the-expression-of-wnt-%C3%AE-catenin-target-genes-in-mouse-reproductive-tissues-during-estrous-cycle-an-experimental-study
#5
Saeed Zavareh, Zahara Gholizadeh, Taghi Lashkarbolouki
Background: The Wingless-type (Wnt)/β-catenin signaling pathway controls cell homeostasis. Reproductive tissues are dynamic in response to steroidal hormone changes. Steroidal hormones are known to control the Wnt/β-catenin pathway, but their role in reproductive tissues remains unknown. Objective: The present study aims to investigate the expression patterns of Wnt/β-catenin target genes in mouse reproductive tissues during the normal estrous cycle. Materials and Methods: In this experimental study, 16 adult NMRI mice were grouped as proestrus, estrus, metestrus, and diestrus according to vaginal smear and histological evaluation of uterine and ovarian tissues...
February 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29673691/laparoscopic-inguinal-ligament-suspension-with-uterine-preservation-for-pelvic-organ-prolapse-a-retrospective-cohort-study
#6
Chunbo Li, Huimin Shu, Zhiyuan Dai
OBJECTIVE: To introduce an alternative surgical technique of laparoscopic inguinal ligament suspension (LILS) with uterine preservation and evaluate its efficacy and safety for patients with severe pelvic organ prolapse (POP). METHODS: Between June 2014 and December 2015, 35 patients with symptomatic stage III or IV were treated by LILS with uterine preservation. The perioperative parameters including surgical time, blood loss, hospital stay and complications were recorded...
April 16, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29624774/multicystic-mucinous-adenocarcinoma-of-the-uterine-cervix-compared-with-benign-multicystic-lesions-multiparametric-mr-features
#7
Jiacheng Song, Qiming Hu, Junwen Huang, Zhanlong Ma, Ting Chen
BACKGROUND: Multicystic mucinous adenocarcinoma is rarely recognized and has a high misdiagnosis rate. PURPOSE: To distinguish malignant multicystic mucinous adenocarcinomas from benign multicystic lesions in the uterine cervix using multiparametric MR. STUDY TYPE: Retrospective. POPULATION: Forty patients with seven cystic mucinous adenocarcinomas and 33 benign multicystic lesions. FIELD STRENGTH/SEQUENCE: 3...
April 6, 2018: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/29571018/stem-cell-therapy-in-asherman-syndrome-and-thin-endometrium-stem-cell-based-therapy
#8
REVIEW
Ramyar Azizi, Leili Aghebati-Maleki, Mohammad Nouri, Faroogh Marofi, Sohrab Negargar, Mehdi Yousefi
The endometrium is one of the essential components of the uterus. The endometrium of human is a complex and dynamic tissue, which undergoes periods of growth and turn over during any menstrual cycle. Stem cells are initially undifferentiated cells that display a wide range of differentiation potential with no distinct morphological features. Stem cell therapy method recently has become a novel procedure for treatment of tissue injury and fibrosis in response to damage. Currently, there is massive interest in stem cells as a novel treatment method for regenerative medicine and more specifically for the regeneration of human endometrium disorder like Asherman syndrome (AS) and thin endometrium...
June 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29544627/the-ovarian-cycle
#9
JoAnne S Richards
The "ovarian cycle" is an exquisite and dynamic endocrine system that includes ovarian events, hypothalamic-pituitary interactions, uterine endometrial and myometrial changes during implantation and pregnancy, cervical alterations in structure, and breast development. The ovarian cycle and the steroid hormones produced by the ovary also impact epithelial cancer development in the ovary, uterus, cervix, and breast. This chapter provides a personal view of recent developments that occur in this complex endocrine environment...
2018: Vitamins and Hormones
https://www.readbyqxmd.com/read/29522888/the-dynamics-of-cortical-folding-waves-and-prematurity-related-deviations-revealed-by-spatial-and-spectral-analysis-of-gyrification
#10
REVIEW
Jessica Dubois, Julien Lefèvre, Hugo Angleys, François Leroy, Clara Fischer, Jessica Lebenberg, Ghislaine Dehaene-Lambertz, Cristina Borradori-Tolsa, François Lazeyras, Lucie Hertz-Pannier, Jean-François Mangin, Petra S Hüppi, David Germanaud
In the human brain, the appearance of cortical sulci is a complex process that takes place mostly during the second half of pregnancy, with a relatively stable temporal sequence across individuals. Since deviant gyrification patterns have been observed in many neurodevelopmental disorders, mapping cortical development in vivo from the early stages on is an essential step to uncover new markers for diagnosis or prognosis. Recently this has been made possible by MRI combined with post-processing tools, but the reported results are still fragmented...
March 6, 2018: NeuroImage
https://www.readbyqxmd.com/read/29519295/role-of-estrogen-and-ras-signaling-in-repeated-implantation-failure
#11
Kwonho Hong, Youngsok Choi
In humans, hormonal regulation is crucial for the preparation of uterine environment leading to either successful implantation or menstrual cycle. Estrogen is a pivotal female steroid hormone that regulates the uterine dynamics along with progesterone in the estrous and menstrual cycles in humans. Estrogen signals act via nuclear estrogen receptor or membrane-bound receptor. The membrane-bound estrogen receptor plays a crucial role in the rapid response of estrogen in the uterine epithelium. Recently, RASD1 has received attention as a novel signal transducer of estrogen in various systems including female reproductive organs...
March 9, 2018: BMB Reports
https://www.readbyqxmd.com/read/29488716/-application-of-dynamic-contrast-enhanced-magnetic-resonance-pharmacokinetic-models-in-differential-diagnosis-of-cellular-uterine-leiomyoma
#12
Subo Wang, Zhenhua Zhao, Hongjie Hu, Jianfeng Yang, Li Zhao, Liming Yang, Cheng Wang
OBJECTIVE: To assess the application of the dynamic-contrast enhanced magnetic resonance imaging(DCE-MRI)pharmacokinetics models in differential diagnosis of cellular uterine leiomyoma. METHODS: Sixty four patients with uterine leiomyoma confirmed by surgery and pathology were enrolled in the study between September 2015 and September 2016, including 30 cases of classical leiomyoma, 13 cases of cellular leiomyoma and 21 cases of degenerative leiomyoma. All patients underwent DCE-MRI before surgery...
May 25, 2017: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/29465714/preeclampsia-induced-by-stox1-overexpression-in-mice-induces-intrauterine-growth-restriction-abnormal-ultrasonography-and-bold-mri-signatures
#13
Hélène Collinot, Carmen Marchiol, Isabelle Lagoutte, Franck Lager, Nathalie Siauve, Gwennhael Autret, Daniel Balvay, Gilles Renault, Laurent J Salomon, Daniel Vaiman
BACKGROUND: Preeclampsia is a major hypertensive disease caused by pregnancy, inducing proteinuria and increased blood pressure starting from the second half of pregnancy (early preeclampsia) or near the end of pregnancy (late preeclampsia). Pre-symptomatic diagnosis would allow for therapeutic interventions, such as with low-dose aspirin. Among non-invasive methods to explore organ physiology, Doppler ultrasonography (US) and functional blood oxygenation level-dependent (BOLD) MRI (which do not need radioactive contrast agents such as gadolinium) can be used in pregnant women...
June 2018: Journal of Hypertension
https://www.readbyqxmd.com/read/29447895/total-congenital-sternal-cleft-isolated-in-a-newborn-of-20-days-rare-case
#14
Dounia Basraoui, Badia Bannar, Mohamed Ouladsaiad, Hicham Jalal
Sternal clefts are rare malformations, especially in their complete form, which results from a lack of fusion of the sternal bars, which is normally done at the 9th week of intra uterine life. The incomplete form is often associated with other malformations of the chest wall, or falling within the framework of a polymalformative syndrome, the diagnosis is easy, being done with the inspection and the palpation, the paraclinical examinations are useful for confirming the diagnosis. We report the case of a total congenital sternal cleft isolated in an asymptomatic 20-day-old newborn...
March 2018: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/29447043/re-engineering-the-interpretation-of-electronic-fetal-monitoring-to-identify-reversible-risk-for-cerebral-palsy-a-case-control-series
#15
Mark I Evans, Robert D Eden, David W Britt, Shara M Evans, Barry S Schifrin
BACKGROUND: Even key opinion leaders now concede that electronic fetal monitoring (EFM) cannot reliably identify fetal acidemia which many vouch as the only labor mediated pathophysiologic precursor for cerebral palsy (CP). We have developed the "Fetal Reserve Index" - an algorithm combining five dynamic components of EFM (1. Rate, 2. Variability, 3. Accelerations, 4. Decelerations, and 5. Excessive uterine activity) considered individually that are combined with the presence of: 6...
February 28, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29433961/immunology-of-the-uterine-and-vaginal-mucosae
#16
REVIEW
Jordan Z Zhou, Sing Sing Way, Kang Chen
Along with the maintenance of symbiotic mutualism with commensal microbes and protection against invasive infections common to all mucosal barrier tissues, female reproductive tissues have additional, unique tasks that include dynamic cyclic cellular turnover in menstruation and immunological tolerance to genetically foreign fetal antigens in pregnancy. Here we review current knowledge on distinct features of the immune cells in female reproductive tissue with regard to antimicrobial host defense and adaptations to accommodate the fetus during pregnancy...
April 2018: Trends in Immunology
https://www.readbyqxmd.com/read/29426931/tridimensional-visualization-reveals-direct-communication-between-the-embryo-and-glands-critical-for-implantation
#17
Jia Yuan, Wenbo Deng, Jeeyeon Cha, Xiaofei Sun, Jean-Paul Borg, Sudhansu K Dey
Embryo implantation is central to pregnancy success. Our previous understanding is limited by studying this phenomenon primarily in two dimensions. Here we employ 3D visualization, revealing that epithelial evaginations that form implantation chambers (crypts) consistently arise with preexisting glands, suggesting direct access of glands to embryos within the chamber. While the lobular domains of the glands become more developed, the ductal regions continue to elongate and progressively stretch following implantation...
February 9, 2018: Nature Communications
https://www.readbyqxmd.com/read/29422425/utility-of-clinical-parameters-and-multiparametric-mri-as-predictive-factors-for-differentiating-uterine-sarcoma-from-atypical-leiomyoma
#18
Qiu Bi, Zhibo Xiao, Fajin Lv, Yao Liu, Chunxia Zou, Yiqing Shen
OBJECTIVES: The objective of this study was to find clinical parameters and qualitative and quantitative magnetic resonance imaging (MRI) features for differentiating uterine sarcoma from atypical leiomyoma (ALM) preoperatively and to calculate predictive values for uterine sarcoma. MATERIALS AND METHODS: Data from 60 patients with uterine sarcoma and 88 patients with ALM confirmed by surgery and pathology were collected. Clinical parameters, qualitative MRI features, diffusion-weighted imaging with apparent diffusion coefficient values, and quantitative parameters of dynamic contrast-enhanced MRI of these two tumor types were compared...
February 5, 2018: Academic Radiology
https://www.readbyqxmd.com/read/29408025/shift-of-uterine-microbiota-associated-with-antibiotic-treatment-and-cure-of-metritis-in-dairy-cows
#19
Soo Jin Jeon, Fabio S Lima, Achilles Vieira-Neto, Vinicius S Machado, Svetlana F Lima, Rodrigo C Bicalho, Jose Eduardo P Santos, Klibs N Galvão
Broad-spectrum antibiotics such as ceftiofur and ampicillin are recommended for the treatment of metritis in dairy cows. Nonetheless, little is known about the impacts of antibiotics on the uterine microbiota. Here, we evaluated the shift in uterine microbiota after treating metritic cows with ceftiofur or ampicillin, and also gained insight into the uterine microbiota associated with cure of metritis. Uterine swabs from ceftiofur-treated, ampicillin-treated, and untreated metritic Holstein cows were collected on the day of metritis diagnosis (D1) and on D6 and then used for genomic DNA extraction and sequencing of the V4 hypervariable region of the 16S rRNA gene on the Illumina MiSeq platform...
February 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29406317/electrohysterography-in-the-diagnosis-of-preterm-birth-a-review
#20
J Garcia-Casado, Y Ye-Lin, G Prats-Boluda, J Mas-Cabo, J Alberola-Rubio, A Perales
Preterm birth (PTB) is one of the most common and serious complications in pregnancy. About 15 million preterm neonates are born every year, with ratios of 10-15% of total births. In industrialized countries, preterm delivery is responsible for 70% of mortality and 75% of morbidity in the neonatal period. Diagnostic means for its timely risk assessment are lacking and the underlying physiological mechanisms are unclear. Surface recording of the uterine myoelectrical activity (electrohysterogram, EHG) has emerged as a better uterine dynamics monitoring technique than traditional surface pressure recordings and provides information on the condition of uterine muscle in different obstetrical scenarios with emphasis on predicting preterm deliveries...
February 26, 2018: Physiological Measurement
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