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M Savolainen, A Ritvanen, J Hukki, P Vuola, J Telkkä, J Leikola
Correction of calvarial defects after calvarial vault reconstruction (CVR) is challenging in craniosynostosis patients of advanced age and typically employs autologous bone. Demineralized bone matrix (DBM) is a potential alternative material for autologous bone, but its use has not been extended to correct calvarial defects. CVR patients operated at the Department of Plastic Surgery, Helsinki University Hospital, during 2008-2010 were retrospectively reviewed. Inclusion criteria of the study were CVR patients who received DBM plate, with or without bone dust, on calvarial defects and who had suitable uncovered defect on the contralateral side as control...
September 20, 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
Ines Westphal, Claudia Jedelhauser, Gregor Liebsch, Arnd Wilhelmi, Attila Aszodi, Matthias Schieker
Bone tissue engineering (BTE) utilizing biomaterial scaffolds and human mesenchymal stem cells (hMSCs) is a promising approach for the treatment of bone defects. The quality of engineered tissue is crucially affected by numerous parameters including cell density and the oxygen supply. In this study, a novel oxygen-imaging sensor was introduced to monitor the oxygen distribution in three dimensional (3D) scaffolds in order to analyze a new cell-seeding strategy. Immortalized hMSCs, pre-cultured in a monolayer for 30-40% or 70-80% confluence, were used to seed demineralized bone matrix (DBM) scaffolds...
October 17, 2016: Biotechnology and Bioengineering
Paolo Giannoni, Federico Villa, Cinzia Cordazzo, Luciano Zardi, Paolo Fattori, Rodolfo Quarto, Mauro Fiorini
Three different heterologous substitutes for bone regeneration, manufactured with equine-derived cortical powder (CP), cancellous chips (CC) and demineralized bone matrix granules (DBM), were compared in in vitro and in vivo settings. We tested: a commercially available bone paste (Osteoplant-Activagen™, consisting of aqueous collagenous carrier, CP, DBM; named A); a second-generation injectable paste (20 kDa polyethylene glycol/hydroxypropyl-methyl cellulose-based hydrogel, CP, DBM; B); a pre-formed bone filler (400 kDa polyethylene oxide/hydroxypropyl-methyl cellulose-based hydrogel, CP, CC, DBM; C)...
November 18, 2016: Biomaterials Science
Jiajia Shi, Jie Sun, Wen Zhang, Hui Liang, Qin Shi, Xiaoran Li, Yanyan Chen, Yan Zhuang, Jianwu Dai
The reconstruction of bone usually depends on substitute transplantation, which has drawbacks including the limited bone substitutes available, comorbidity, immune rejection, and limited endogenous bone regeneration. Here, we constructed a functionalized bone substitute by combining application of the demineralized bone matrix (DBM) and collagen-binding stromal-cell-derived factor-1α (CBD-SDF-1α). DBM was a poriferous and biodegradable bone substitute, derived from bovine bone and consisting mainly of collagen...
October 7, 2016: ACS Applied Materials & Interfaces
Eva Kvasničková, Vít Paulíček, Martina Paldrychová, Richard Ježdík, Olga Maťátková, Jan Masák
The biofilms of filamentous-forming fungi are a novel and still insufficiently understood research topic. We have studied Aspergillus fumigatus, an ubiquitous opportunistic pathogenic fungus, as a representative model for a study of biofilm formation by filamentous fungi and for assessing the potential anti-biofilm activity of natural substances. The activity of antibiotic amphotericin B and selected natural substances: baicalein, chitosan and rhamnolipid was studied. The minimum suspension inhibitory concentrations (MIC) were determined and the biofilm susceptibility was investigated by determining the metabolic activity of sessile cells (XTT assay) and total biofilm biomass (crystal violet staining)...
November 2016: World Journal of Microbiology & Biotechnology
Nikhil D Chandradas, Shivamurthy Ravindra, Vivekananda M Rangaraju, Sheetal Jain, Shivaprasad Dasappa
AIM: To evaluate the efficacy of platelet rich fibrin (PRF) with or without bone graft [demineralized bone matrix (DBM) graft] in the treatment of intrabony defects based on clinical and radiographic parameters. MATERIALS AND METHODS: Thirty six intrabony defects in 36 patients were randomly divided into three different groups and were treated with group A (PRF with DBM) or group B (PRF alone) or group C [open flap debridement (OFD)]. Clinical parameters such as plaque index (PI), gingival index (GI), probing depth (PD), relative attachment level (RAL), and gingival recession (GR) were assessed at baseline and 9 months postoperatively; radiographic parameters such as linear bone growth (LBG) and percentage in bone fill (%BF) were calculated by using the image analysis software...
August 2016: Journal of International Society of Preventive & Community Dentistry
Zhentao Man, Xiaoqing Hu, Zhenlong Liu, Hongjie Huang, Qingyang Meng, Xin Zhang, Linghui Dai, Jiying Zhang, Xin Fu, Xiaoning Duan, Chunyan Zhou, Yingfang Ao
Cartilage tissue engineering is the hotspot of cartilage repair. The allogenic chondrocytes appear to be a promising source of seed cells in cartilage tissue engineering. In this study, we aimed to transplant allogenic chondrocytes with chitosan hydrogel (CS)-demineralized bone matrix (DBM) hybrid scaffold (CS/DBM) to repair rabbit cartilage injury with one-step operation. After the CS/DBM scaffold was successfully fabricated, it showed that the porous CS filled the large pores of DBM, which improved the distribution of seed cells in the CS/DBM scaffold...
November 2016: Biomaterials
Marek Bolanowski, Wojciech Pluskiewicz, Joanna Syrycka, Agnieszka Chwałczyńska
BACKGROUND: Growing of the skeleton, depends on genetic, health, nutritional and environmental influences. OBJECTIVES: The aim of the study was to assess the ultrasound properties of bone measured at hand phalanges in adolescent girls regarding the influence of pubertal status and level of physical fitness. MATERIAL AND METHODS: The study group was 56 adolescent girls aged 12-14 yrs, the controls were 86 girls matched for age, recruited from 1256 subjects undergoing bone measurement for screening purposes...
March 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Biqiang Jiang, Meng Xue, Chenyang Zhao, Dong Mao, Kaiming Zhou, Lin Zhang, Jianlin Zhao
A carbon nanotube (CNT)-modified microfiber Bragg grating (MFBG) is proposed to measure the refractive index with a strong enhancement of the sensitivity in the low refractive index region. The introduction of the CNT layer influences the evanescent field of the MFBG and causes modification of the reflection spectrum. With the increase of the surrounding refractive index (SRI), we observe significant attenuation to the peak of the Bragg resonance, while its wavelength remains almost unchanged. Our detailed experimental results disclose that the CNT-MFBG demonstrates strong sensitivity in the low refractive index range of 1...
September 1, 2016: Applied Optics
Yaxue Zhai, Xianfeng Tang, Xiaoguang Zhang, Lixia Xi, Wenbo Zhang
This paper investigates the performance of an all-optical logic gate scheme based on a single in-phase and quadrature (I/Q) modulator with direct detection. The proposed scheme of an all-optical logic gate is simple, high speed, and easily reconfigured to realize 24 logic states by adjusting bias voltages, peak-to-peak voltages of the driven RF signals, and the phase shift. As the scheme to realize logic gates is based on the irregular use of a commercially available I/Q modulator and laser source, a specialized logic gate system including a laser, I/Q modulator, and driven RF module should be optimally designed to obtain the best performance...
September 1, 2016: Applied Optics
Kathleen M Fleming, Jennifer C Pekar, Brian D Leskiw
RATIONALE: While much of the interest and application of beta-diketonate complexes has focused predominately on the condensed phases, we report on the prevalence of gas-phase ligand exchange reactions leading to the formation of novel species. METHODS: Gas-phase ligand exchange reactions of Mg(eeac)2 and Mg(tftm)2 complexes with Cu(acac)2 , Cu(tftm)2 , Cu(dbm)2 , and Cu(eeac)2 were investigated using a direct insertion electron impact triple quadrupole mass spectrometer...
November 30, 2016: Rapid Communications in Mass Spectrometry: RCM
Vincent Giordano, Christophe Fluhr, Benoît Dubois, Enrico Rubiola
We present the characterization of commercial tunnel diode low-level microwave power detectors at room and cryogenic temperatures. The sensitivity as well as the output voltage noise of the tunnel diodes is measured as functions of the applied microwave power. We highlight strong variations of the diode characteristics when the applied microwave power is higher than a few microwatts. For a diode operating at 4 K, the differential gain increases from 1000 V/W to about 4500 V/W when the power passes from -30 dBm to -20 dBm...
August 2016: Review of Scientific Instruments
I Juric, W Salzburger, O Balmer
The diamondback moth (DBM) (Plutella xylostella) is one of the main pests of brassicaceous crops worldwide and shows resistance against a wide range of synthetic insecticides incurring millions of dollars in control costs every year. The DBM is a prime example of the introduction of an exotic species as a consequence of globalization. In this study we analyzed the genetic population structure of the DBM and two of its parasitic wasps, Diadegma semiclausum and Diadegma fenestrale, based on mitochondrial DNA sequences...
August 30, 2016: Bulletin of Entomological Research
Mark Schallenberger, Helena Lovick, Jalane Locke, Todd Meyer, Gregory Juda
During August and September of 2013, temperature data loggers were shipped to and from an AATB accredited and FDA registered allograft tissue processing facility in Belgrade, MT (Bacterin International, Inc.) to five warm climate cities (Dallas, TX, El Paso, TX, New Orleans, LA, Phoenix, AZ, and Tampa, FL). Shipping data acquired from 72 independent shipments were analyzed to generate an assessment of temperature exposure, shipment times, and shipping event durations experienced during routine distribution...
August 25, 2016: Cell and Tissue Banking
Hong-Ling Gao, Li Jiang, Wen-Min Wang, Shi-Yu Wang, Hong-Xia Zhang, Jian-Zhong Cui
Five tetranuclear rare-earth complexes, [RE4(dbm)4L6(μ3-OH)2] [HL = 5- (4-fluorobenzylidene)-8-hydroxylquinoline; dbm = 1,3-diphenyl-1,3-propanedione; RE = Y (1), Eu (2), Tb (3), Dy (4), Lu (5)], have been synthesized and completely characterized. The X-ray structural analyses show that each [RE4] complex is of typical butterfly or rhombus topology. Each RE(III) center exists in an eight-coordinated square-antiprism environment. Magnetic studies reveal that complex 4 displays single-molecule-magnet behavior below 10 K under a zero direct-current field, with an effective anisotropy barrier (ΔE/kB = 56 K)...
September 6, 2016: Inorganic Chemistry
Nicholas J D Martinez, Christopher T Derose, Reinhard W Brock, Andrew L Starbuck, Andrew T Pomerene, Anthony L Lentine, Douglas C Trotter, Paul S Davids
We present experimental results for a selective epitaxially grown Ge-on-Si separate absorption and charge multiplication (SACM) integrated waveguide coupled avalanche photodiode (APD) compatible with our silicon photonics platform. Epitaxially grown Ge-on-Si waveguide-coupled linear mode avalanche photodiodes with varying lateral multiplication regions and different charge implant dimensions are fabricated and their illuminated device characteristics and high-speed performance is measured. We report a record gain-bandwidth product of 432 GHz for our highest performing waveguide-coupled avalanche photodiode operating at 1510nm...
August 22, 2016: Optics Express
Sompop Bencharit, Riley K Allen, Daniel Whitley
Insufficient bucco-lingual bone often limits the implant placement rendering bone and soft tissue regeneration/augmentation. The biological and clinical aspects of demineralized bone matrix (DBM) for augmentation with implant placement were reviewed and a clinical report was presented. A 38-year-old Hispanic female presented with missing a mandibular second premolar. Pre-operative cone beam computed tomography (CBCT) scans showed deficiency in the buccal bone. An endossesous dental implant and DBM allograft was placed with a custom healing abutment...
August 18, 2016: Journal of Oral Implantology
Juan Clement, Germán Torregrosa, Haroldo Maestre, Carlos R Fernández-Pousa
We report on the self-referenced, intensity-based, remote and passive interrogation of a fiber Bragg grating (FBG) for point sensing, by use of a reconfigurable dual-wavelength source composed of a tunable wavelength and subsequent suppressed-carrier, electro-optic amplitude modulation. The demodulation procedure is based on the measurement of the reflected power at two different wavelengths within the FBG spectral response. The grating was interrogated by use of conventional spectral analysis, and also after 32...
August 10, 2016: Applied Optics
Shuzhen Zhang, Xiaolei Zhang, Jun Shen, Kaikai Mao, Hong You, Jianhong Li
The diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a globally distributed and important economic pest. Chemical control is the primary approach to regulate populations of this pest. However, resistance to insecticides evolves following heavy and frequent use. Therefore, the insecticide resistance in field populations of P. xylostella collected from Central China from 2013 to 2014 was determined with a leaf-dipping method. Based on the results of the monitoring, P. xylostella has developed high levels of resistance to beta-cypermethrin (resistance ratio=69...
September 2016: Pesticide Biochemistry and Physiology
Li Fan, Guangqiong Xia, Jianjun Chen, Xi Tang, Qing Liang, Zhengmao Wu
Based on an optically injected semiconductor laser (OISL) operating at period-one (P1) nonlinear dynamical state, high-purity millimeter-wave generation at 60 GHz band is experimentally demonstrated via 1/4 and 1/9 subharmonic microwave modulation (the order of subharmonic is with respect to the frequency fc of the acquired 60 GHz band millimeter-wave but not the fundamental frequency f0 of P1 oscillation). Optical injection is firstly used to drive a semiconductor laser into P1 state. For the OISL operates at P1 state with a fundamental frequency f0 = 49...
August 8, 2016: Optics Express
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