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https://www.readbyqxmd.com/read/28345343/electronic-and-steric-optimization-of-fluorogenic-probes-for-biomolecular-imaging
#1
Wen Chyan, Henry R Kilgore, Brian Gold, Ronald T Raines
Fluorogenic probes are invaluable tools for spatiotemporal investigations within live cells. In common fluorogenic probes, the intrinsic fluorescence of a small-molecule fluorophore is masked by esterification until entry into a cell, where endogenous esterases catalyze the hydrolysis of the masking groups, generating fluorescence. The susceptibility of masking groups to spontaneous hydrolysis is a major limitation of these probes. Previous attempts to address this problem have incorporated auto-immolative linkers at the cost of atom economy and synthetic adversity...
March 27, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28344635/the-combination-of-gas-phase-fluorophore-technology-and-automation-to-enable-high-throughput-analysis-of-plant-respiration
#2
Andrew P Scafaro, A Clarissa A Negrini, Brendan O'Leary, F Azzahra Ahmad Rashid, Lucy Hayes, Yuzhen Fan, You Zhang, Vincent Chochois, Murray R Badger, A Harvey Millar, Owen K Atkin
BACKGROUND: Mitochondrial respiration in the dark (Rdark) is a critical plant physiological process, and hence a reliable, efficient and high-throughput method of measuring variation in rates of Rdark is essential for agronomic and ecological studies. However, currently methods used to measure Rdark in plant tissues are typically low throughput. We assessed a high-throughput automated fluorophore system of detecting multiple O2 consumption rates. The fluorophore technique was compared with O2-electrodes, infrared gas analysers (IRGA), and membrane inlet mass spectrometry, to determine accuracy and speed of detecting respiratory fluxes...
2017: Plant Methods
https://www.readbyqxmd.com/read/28344357/quantum-dot-surface-engineering-toward-inert-fluorophores-with-compact-size-and-bright-stable-emission
#3
Sung Jun Lim, Liang Ma, André Schleife, Andrew M Smith
The surfaces of colloidal nanocrystals are complex interfaces between solid crystals, coordinating ligands, and liquid solutions. For fluorescent quantum dots, the properties of the surface vastly influence the efficiency of light emission, stability, and physical interactions, and thus determine their sensitivity and specificity when they are used to detect and image biological molecules. But after more than 30 years of study, the surfaces of quantum dots remain poorly understood and continue to be an important subject of both experimental and theoretical research...
August 1, 2016: Coordination Chemistry Reviews
https://www.readbyqxmd.com/read/28344061/compartmentation-of-mitochondrial-and-oxidative-metabolism-in-growing-hair-follicles-a-ring-of-fire
#4
John J Lemasters, Venkat K Ramshesh, Gregory L Lovelace, John Lim, Graham D Wright, Duane Harland, Thomas L Dawson
Little is known about the energetics of growing hair follicles, particularly in the mitochondrially abundant bulb. Here, mitochondrial and oxidative metabolism was visualized by multiphoton and light sheet microscopy in cultured bovine hair follicles and plucked human hairs. Mitochondrial membrane potential (ΔΨ), cell viability, reactive oxygen species (ROS) and secretory granules were assessed with parameter-indicating fluorophores. In growing follicles, lower bulb epithelial cells had high viability, and mitochondria were polarized...
March 23, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28342426/optical-and-electrochemical-dual-channel-sensing-of-cu-2-using-functionalized-furo-2-3-d-pyrimidines-2-4-1h-3h-diones
#5
Manoj Kumar, Lokesh Kumar Kumawat, Priyanka Bhatt, Anjali Jha, Shilpi Agarwal, Anuj Sharma, Vinod Kumar Gupta
Owing to their easy accessibility and high degree of structural and functional diversity, many multicomponent reactions (MCRs) have been a rich source of conjugate π-systems, functionalised chromophores (or fluorophore) and redox active molecules. Despite their high explorative potential and practical benefits, only a few MCR products have been so far investigated for their metal sensing abilities. In the present report, two furopyrimidinones (FPys) based molecular systems have been synthesized by [4+1] cycloaddition based MCR sequence...
March 18, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28340733/migration-test-of-bisphenol-a-from-polycarbonate-cups-using-excitation-emission-fluorescence-data-with-parallel-factor-analysis
#6
M L Spagnuolo, F Marini, L A Sarabia, M C Ortiz
Bisphenol A (BPA) is one of the most largely produced chemical in the world; it is used to make plastics and epoxy resins. The endocrine disruptor potential of BPA is well known, but recent researches suggest a relationship between chronic exposure to BPA, genotoxic activity and epigenetic modifications. The main source of exposure to BPA includes food contact materials (FCM). Thus simple and robust test methods are needed to improve the migration test of BPA. In this work, a non-separative, easy, fast and inexpensive spectrofluorimetric method based on the second order calibration of excitation-emission fluorescence matrices (EEMs) was proposed for the determination of BPA...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28339076/an-activatable-aptamer-based-fluorescence-probe-for-the-detection-of-hepg2-cells
#7
Zongqiang Lai, Juntao Tan, Ruirong Wan, Jie Tan, Zhenghua Zhang, Zixi Hu, Jieping Li, Wei Yang, Yiwei Wang, Yafeng Jiang, Jian He, Nuo Yang, Xiaoling Lu, Yongxiang Zhao
It is significant to develop a probe with sensitivity and specificity for the detection of cancer cells. The present study aimed to develop an 'activatable' aptamer-based fluorescence probe (AAFP) to detect cancer cells and frozen cancer tissue. This AAFP consisted of two fragments: aptamer TLS11a that targets HepG2 cells, and two short extending complementary DNA sequences with a 5'- and 3'-terminus that make the aptamer in hairpin structure a capable quencher to fluorophore. The ability of the AAFP to bind specifically to cancer cells was assessed using flow cytometry, fluorescence spectroscopy and fluorescence microscopy...
March 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28338043/redshifted-cherenkov-radiation-for-in-vivo-imaging-coupling-cherenkov-radiation-energy-transfer-to-multiple-f%C3%A3-rster-resonance-energy-transfers
#8
Yann Bernhard, Bertrand Collin, Richard A Decréau
Cherenkov Radiation (CR), this blue glow seen in nuclear reactors, is an optical light originating from energetic β-emitter radionuclides. CR emitter (90)Y triggers a cascade of energy transfers in the presence of a mixed population of fluorophores (which each other match their respective absorption and emission maxima): Cherenkov Radiation Energy Transfer (CRET) first, followed by multiple Förster Resonance Energy transfers (FRET): CRET ratios were calculated to give a rough estimate of the transfer efficiency...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28335209/dye-doped-fluorescent-silica-nanoparticles-for-live-cell-and-in-vivo-bioimaging
#9
REVIEW
Wen-Han Zhang, Xiao-Xiao Hu, Xiao-Bing Zhang
The need for novel design strategies for fluorescent nanomaterials to improve our understanding of biological activities at the molecular level is increasing rapidly. Dye-doped fluorescent silica nanoparticles (SiNPs) emerge with great potential for developing fluorescence imaging techniques as a novel and ideal platform for the monitoring of living cells and the whole body. Organic dye-containing fluorescent SiNPs exhibit many advantages: they have excellent biocompatibility, are non-toxic, highly hydrophilic, optically transparent, size-tunable and easily modified with various biomolecules...
April 27, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334446/molecular-glycopathology-by-capillary-electrophoresis-analysis-of-the-n-glycome-of-formalin-fixed-paraffin-embedded-mouse-tissue-samples
#10
Boglarka Donczo, Mate Szarka, Jozsef Tovari, Gyorgyi Ostoros, Eszter Csanky, Andras Guttman
Capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was used to analyze endoglycosidase released and fluorophore-labeled N-glycans from formalin-fixed paraffin-embedded (FFPE) mouse tissue samples of lung, brain, heart, spleen, liver, kidney and intestine. The FFPE samples were first deparaffinized followed by solubilization and glycoprotein retrieval. PNGase F mediated release of the N-linked oligosaccharides was followed by labeling with aminopyrene trisulfonate. After CE-LIF glycoprofiling of the FFPE mouse tissues, the N-glycan pool of the lung specimen was subject to further investigation by exoglycosidase array based carbohydrate sequencing...
March 23, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28333166/effect-of-probe-diffusion-on-the-sofi-imaging-accuracy
#11
Wim Vandenberg, Peter Dedecker
Live-cell super-resolution fluorescence imaging is becoming commonplace for exploring biological systems, though sample dynamics can affect the imaging quality. In this work we evaluate the effect of probe diffusion on super-resolution optical fluctuation imaging (SOFI), using a theoretical model and numerical simulations based on the imaging of live cells labelled with photochromic fluorescent proteins. We find that, over a range of physiological conditions, fluorophore diffusion results in a change in the amplitude of the SOFI signal...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332834/multicomponent-platinum-ii-cages-with-tunable-emission-and-amino-acid-sensing
#12
Mingming Zhang, Manik Lal Saha, Ming Wang, Zhixuan Zhou, Bo Song, Chenjie Lu, Xuzhou Yan, Xiaopeng Li, Feihe Huang, Shouchun Yin, Peter J Stang
The syntheses, characterization and the emission properties of three tetragonal prismatic cages 4a-4c constructed from eight 90o Pt(II) acceptors, four linear dipyridyl ligands and two tetraphenylethene (TPE) based sodium benzoate ligands are described. These cages are emissive in dilute solutions due to the metal-coordination induced partial restriction of intramolecular rotation of their TPE units, while the dipyridyl moieties which act as the pillars as well as the solvents strongly influence these emissions...
March 23, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28332258/n-terminal-chemical-protein-labeling-using-the-naturally-split-gos-terl-intein
#13
Anne-Lena Bachmann, Henning D Mootz
Chemoselective and regioselective chemical protein labeling is of great importance, yet no current technique is sufficiently general and simple to perform. Protein trans-splicing by split inteins can be used to ligate short tags with chemical labels to either the N or the C terminus of a protein. The CysTag approach exploits split intein fragments without a cysteine fused with such a short tag containing a single cysteine that is easily amenable to selective modification using classical cysteine bioconjugation...
March 23, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28330937/raft-based-sphingomyelin-interactions-revealed-by-new-fluorescent-sphingomyelin-analogs
#14
Masanao Kinoshita, Kenichi G N Suzuki, Nobuaki Matsumori, Misa Takada, Hikaru Ano, Kenichi Morigaki, Mitsuhiro Abe, Asami Makino, Toshihide Kobayashi, Koichiro M Hirosawa, Takahiro K Fujiwara, Akihiro Kusumi, Michio Murata
Sphingomyelin (SM) has been proposed to form cholesterol-dependent raft domains and sphingolipid domains in the plasma membrane (PM). How SM contributes to the formation and function of these domains remains unknown, primarily because of the scarcity of suitable fluorescent SM analogs. We developed new fluorescent SM analogs by conjugating a hydrophilic fluorophore to the SM choline headgroup without eliminating its positive charge, via a hydrophilic nonaethylene glycol linker. The new analogs behaved similarly to the native SM in terms of their partitioning behaviors in artificial liquid order-disorder phase-separated membranes and detergent-resistant PM preparations...
March 22, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28328630/fluorescein-isothiocyanate-a-novel-application-for-lymphatic-surgery
#15
Lisa Spiguel, Christiana Shaw, Adam Katz, Lifei Guo, Hung-Chi Chen, Bernard T Lee, Dhruv Singhal
The Lymphatic Microsurgical Preventing Healing Approach (LYMPHA) procedure entails performing a lymphovenous bypass (LVB) at the time of axillary lymph node dissection to reduce lymphedema risk. The two most common fluorophores utilized in LVB are blue dye and indocyanine green. We developed a novel application of fluorescein isothiocyanate for intraoperative lymphatic mapping. Our goal is to demonstrate the safety and efficacy of fluorescein isothiocyanate for this application. We reviewed a prospectively collected database on breast cancer patients who underwent LYMPHA from March to September 2015...
March 21, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28328542/an-excited-state-intramolecular-photon-transfer-fluorescence-probe-for-localizable-live-cell-imaging-of-cysteine
#16
Wei Liu, Wen Chen, Si-Jia Liu, Jian-Hui Jiang
Small molecule probes suitable for selective and specific fluorescence imaging of some important but low-concentration intracellular reactive sulfur species such as cysteine (Cys) pose a challenge in chemical biology. We present a readily available, fast-response fluorescence probe CHCQ-Ac, with 2-(5'-chloro-2-hydroxyl-phenyl)-6-chloro-4(3 H)-quinazolinone (CHCQ) as the fluorophore and acrylate group as the functional moiety, that enables high-selectivity and high-sensitivity for detecting Cys in both solution and biological system...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328541/molecular-engineering-of-two-photon-fluorescent-probes-for-bioimaging-applications
#17
Hong-Wen Liu, Yongchao Liu, Peng Wang, Xiao-Bing Zhang
During the past two decades, two-photon microscopy (TPM), which utilizes two near-infrared photons as the excitation source, has emerged as a novel, attractive imaging tool for biological research. Compared with one-photon microscopy, TPM offers several advantages, such as lowering background fluorescence in living cells and tissues, reducing photodamage to biosamples, and a photobleaching phenomenon, offering better 3D spatial localization, and increasing penetration depth. Small-molecule-based two-photon fluorescent probes have been well developed for the detection and imaging of various analytes in biological systems...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328200/fluorescence-resonance-energy-transfer-based-dna-tetrahedron-nano-tweezer-for-highly-reliable-detection-of-tumor-related-mrna-in-living-cells
#18
Lei He, Dan-Qing Lu, Hao Liang, Sitao Xie, Can Luo, Miaomiao Hu, Liujun Xu, Xiaobing Zhang, Weihong Tan
Accurate detection and imaging of tumor-related mRNA in living cells hold great promise for early cancer detection. However, currently, most probes designed to image intracellular mRNA confront intrinsic interferences arising from complex biological matrices and resulting in inevitable false-positive signals. To circumvent this problem, a new class of intracellular DNA nanoprobe, termed DNA tetrahedron nano-tweezer (DTNT), was developed to reliably image tumor-related mRNA in living cells based on the FRET (fluorescence resonance energy transfer) "off" to "on" signal readout mode...
March 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28328030/estimation-of-microscope-drift-using-fluorescent-nanodiamonds-as-fiducial-markers
#19
W Colomb, J Czerski, J D Sau, S K Sarkar
Fiducial markers are used to correct the microscope drift and should be photostable, be usable at multiple wavelengths and be compatible for multimodal imaging. Fiducial markers such as beads, gold nanoparticles, microfabricated patterns and organic fluorophores lack one or more of these criteria. Moreover, the localization accuracy and drift correction can be degraded by other fluorophores, instrument noise and artefacts due to image processing and tracking algorithms. Estimating mechanical drift by assuming Gaussian distributed noise is not suitable under these circumstances...
March 22, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28326794/urothelial-tumors-and-dual-band-imaging-a-new-concept-in-confocal-laser-endomicroscopy
#20
Arnaud Marien, Aurélien Rock, Khaled El Maadarani, Catherine Francois, Pierre Gosset, Brigitte Mauroy, Jean-Louis Bonnal
OBJECTIVES: Confocal laser endomicroscopy (CLE) uses a low-energy laser light source to obtain microscopic histology images of bladder tissue exposed to a fluorescent dye. To evaluate the feasibility of using CLE with two fluorophores: fluorescein (FLUO) and hexylaminolevulinate (HAL) to determine histologic and cytologic bladder cancer criteria. METHODS: Patients eligible for HAL-photodynamic diagnosis-assisted transurethral resection of bladder tumor were included...
March 22, 2017: Journal of Endourology
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