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https://www.readbyqxmd.com/read/28434063/constrained-photophysics-of-5-7-dimethoxy-2-3-4-9-tetrahydro-1h-carbazol-1-one-in-the-bioenvironment-of-serum-albumins-a-spectroscopic-endeavour-supported-by-molecular-docking-analysis
#1
Amrit Krishna Mitra, Abhishek Sau, Uttam Pal, Chandan Saha, Samita Basu
This paper vividly indicates that steady state as well as time-resolved fluorescence techniques can serve as highly sensitive monitors to explore the interactions of 5,7-dimethoxy-2,3,4,9-tetrahydro-1H-carbazol-1-one with model transport proteins, bovine serum albumin (BSA) and human serum albumin (HSA). Besides these, we have used fluorescence anisotropy study to assess the degree of restrictions imparted by the micro-environments of serum albumins. Again, to speculate the triplet excited state interaction between such fluorophore and albumin proteins (BSA& HSA), laser flash-photolysis experiments have been carried out...
April 22, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28434062/nlophoric-3-6-di-substituted-quinoxalin-carbazoles-synthesis-photophysical-properties-and-dft-studies
#2
Rahul D Telore, Amol G Jadhav, Nagaiyan Sekar
Synthesis of novel 3,6-di(substituted quinoxalin) carbazole fluorophores by the condensation of 1,1'-(9-ethyl-9H-carbazole-3,6-diyl)bis(2-bromoethanone) with methyl, chloro and unsubstituted o-phenylenediamine is presented. Synthesized derivatives are well characterized by (1)H NMR, (13)C NMR, FTIR and Mass spectroscopy. Photophysical studies are carried out using solvents of varying polarities revealed positive solvatochromism and intramolecular charge transfer from carbazole (Donor) to quinoxalin (Acceptor)...
April 22, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28432919/synthesis-fluorescence-sensing-and-molecular-logic-of-two-water-soluble-1-8-naphthalimides
#3
Nikolai I Georgiev, Margarita D Dimitrova, Anelia Ts Mavrova, Vladimir B Bojinov
Two novel highly water-soluble fluorescence sensing 1,8-naphthalimides are synthesized and investigated. The novel compounds are designed on the "fluorophore-receptor1-spacer-receptor2" model as a molecular fluorescence probe for determination of cations and anions in 100% aqueous media. The novel probes comprising N-imide and N-phenylpiperazine or morpholine substituents are capable to operate simultaneously via ICT and PET signaling mechanism as a function of pH and to recognize selectively Cu(2+) and Hg(2+) over the other representative metal ions...
April 18, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28432259/probing-native-metal-ion-association-sites-through-quenching-of-fluorophores-in-the-nucleotide-binding-domains-of-the-abc-transporter-msba
#4
Daiki Tatsumi, Kei Nanatani, Yuto Koike, Kiyoto Kamagata, Satoshi Takahashi, Ayumu Konno, Tadaomi Furuta, Minoru Sakurai, Nobuyuki Uozumi
ATP binding cassette (ABC) transporters are ubiquitously present in prokaryotic and eukaryotic cells. Binding of ATP to the nucleotide binding domains (NBDs) elicits major conformational changes of the transporters resulting in the transport of the substrate across the membrane. The availability of a crystal structure of the NBDs enabled us to elucidate the local structure and small-scale dynamics in the NBDs. Here, we labeled the ABC transporter MsbA, a homodimeric flippase from E. coli , with a fluorescent probe, Alexa532, within the NBDs...
April 21, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28430413/bioimaging-with-macromolecular-probes-incorporating-multiple-bodipy-fluorophores
#5
Ek Raj Thapaliya, Yang Zhang, Pravat Dhakal, Adrienne S Brown, James N Wilson, Kevin Collins, Françisco M Raymo
Seven macromolecular constructs incorporating multiple borondipyrromethene (BODIPY) fluorophores along a common poly(methacrylate) backbone with decyl and oligo(ethylene glycol) side chains were synthesized. The hydrophilic oligo(ethylene glycol) components impose solubility in aqueous environment on the overall assembly. The hydrophobic decyl chains insulate effectively the fluorophores from each other to prevent detrimental interchromophoric interactions and preserve their photophysical properties. As a result, the brightness of these multicomponent assemblies is approximately three times greater than that of a model BODIPY monomer...
April 21, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28428991/a-room-temperature-phosphorescent-polymer-film-containing-a-molecular-web-based-on-one-dimensional-chiral-stacking-of-a-simple-luminophore
#6
Kyohei Yoshida, Yutaka Kuwahara, Koji Miyamoto, Seiya Nakashima, Hirokuni Jintoku, Makoto Takafuji, Hirotaka Ihara
We propose a new approach for a totally organic, room-temperature phosphorescent system with a lifetime on the order of milliseconds, which is achieved by promotion of the one-dimensional chiral stacking of a simple, small fluorophore in a solution state. The versatility of this system is highlighted by its good applicability to the fabrication of a phosphorescent polymer film. This paper demonstrates the first example of a room-temperature phosphorescent polymer film prepared with our methodology.
April 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28427878/localized-degradation-of-foreign-dna-strands-in-cells-only-excising-the-first-nucleotide-of-5-region
#7
Hui Li, Wei Shen, Michael Hon-Wah Lam, Haojun Liang
Intracellular delivery of foreign DNA probes sharply increases the efficiency of various biodetection protocols. Spherical nucleic acid (SNA) conjugate is a new type of probe that consists of a dense oligonucleotide shell attached typically to a gold nanoparticle core. They are widely used as novel labels for in vitro biodetection and intracellular assay. However, the degradation of foreign DNA still remains a challenge that can cause significant signal leakage (false positive signal). Hence, the site and behavior of intracellular degradation need to be investigated...
April 17, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28425548/emission-behaviours-of-novel-v-and-x-shaped-fluorophores-in-response-to-ph-and-force-stimuli
#8
Hong-Yu Fu, Ning Xu, Yi-Min Pan, Xiao-Lin Lu, Min Xia
A family of novel fluorophores with V- and X-shaped skeletons is rationally designed and prepared. Due to the extended cross π-conjugation in the X-shaped ones, their emission wavelengths in both solution and the solid state exhibit considerable bathochromic shifts compared with those of the V-shaped ones. It is found that a heavy intramolecular charge transfer (ICT) effect is the primary element for the mechanofluorochromic (MFC) response of this family, and an appropriate crystallinity of the sample is also indispensible for it to be MFC-active...
April 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28421434/estimation-of-fluorescence-lifetime-of-lipofuscin-fluorophores-contained-in-lipofuscin-granules-of-retinal-pigment-epithelium-of-human-cadaver-eyes-without-signs-of-pathology
#9
M A Yakovleva, T B Feldman, P M Arbukhanova, S A Borzenok, V A Kuzmin, M A Ostrovsky
The fluorescence lifetimes of lipofuscin fluorophores contained in chloroform extracts from retinal pigment epithelium (RPE) of human cadaver eyes without signs of pathology were evaluated by single photon counting. The comparison of fluorescence lifetimes of N-retinylidene-N-retinylethanolamine (A2E) and its photooxidation and photodegradation products has been carried out. It was shown that the contribution of A2E to the total fluorescence of chloroform extract from lipofuscin granules is not major. The results are important for the improvement of noninvasive diagnostic method of degenerative diseases of the retina and RPE-fundus autofluorescence (FAF)...
January 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28421322/a-colorimetric-and-fluorescent-probe-based-on-michael-acceptor-type-diketopyrrolopyrrole-for-cyanide-detection
#10
Lingyun Wang, Shaochun Zhou, Derong Cao
A new probe 1 was synthesized by incorporating an α,β-unsaturated ketone to a diketopyrrolopyrrole fluorophore. The probe exhibited a selective and sensitive response to cyanide against other anions. Addition of CN(-) aqueous solution to 1 resulted in a rapid color change from pink to light yellow together with a blue shift from 518 to 421 nm, while other anions did not induce any significant color change. Furthermore, the Michael addition of cyanide to 1 elicited 98% fluorescence quenching at 608 nm, which constituted the fluorescence signature for cyanide detection...
April 18, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28421321/experimental-investigations-on-fluorescence-excitation-and-depletion-of-carbon-dots
#11
Yunxia Wang, Zhenhua Bai, Qian Wang, Guiren Wang
Carbon dots (CDs) can be readily synthesized and utilized as attractive fluorescent probes for a variety of applications. In this study, we have synthesized CDs using a previously published method and characterized their photo-physical properties. The resultant CDs possess prominent photo-stability and short emission wavelength in the violet region. Our study reveals that CDs, with weak photo-bleaching, enable them to be employed to achieve high spatial resolution in stimulated emission depletion (STED) microscopy...
April 18, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28421220/a-highly-selective-colorimetric-and-environment-sensitive-optical-potassium-ion-sensor
#12
Guangjie Song, Ruofan Sun, Jiqing Du, Meiwan Chen, Yanqing Tian
Potassium ions (K(+)) play vital roles in many biological processes and thus highly selective sensors for K(+) are critical for disease diagnosis and health monitoring. Herein, we report a colorimetric K(+) sensor (KS7) in which a hemicyanine dye was used as a fluorophore and phenylaza-[18]crown-6 lariat ether (ACLE) was utilized as a K(+) ligand. The maximum absorption peak of KS7 shifted hypsochromically by 77 nm (from 515 to 438 nm) with an isosbestic point at 452 nm upon the addition of K(+) to its aqueous solution accompanied by a color change from red to yellow...
April 19, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28417977/multicolour-nanoscopy-of-fixed-and-living-cells-with-a-single-sted-beam-and-hyperspectral-detection
#13
Franziska R Winter, Maria Loidolt, Volker Westphal, Alexey N Butkevich, Carola Gregor, Steffen J Sahl, Stefan W Hell
The extension of fluorescence nanoscopy to larger numbers of molecular species concurrently visualized by distinct markers is of great importance for advanced biological applications. To date, up to four markers had been distinguished in STED experiments featuring comparatively elaborate imaging schemes and optical setups, and exploiting various properties of the fluorophores. Here we present a simple yet versatile STED design for multicolour imaging below the diffraction limit. A hyperspectral detection arrangement (hyperSTED) collects the fluorescence in four spectral channels, allowing the separation of four markers with only one excitation wavelength and a single STED beam...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28417373/a-rapid-procedure-for-the-purification-of-8-aminopyrene-trisulfonate-apts-labeled-glycans-for-capillary-electrophoresis-ce-based-enzyme-assays
#14
Hayden J Danyluk, Leona K Shum, Wesley F Zandberg
Purified glycan standards are required for glycan arrays, characterizing substrate specificities of glycan-active enzymes, and to serve as retention-time or mobility standards for various separation techniques. This chapter describes a method for the rapid separation, and subsequent desalting, of glycans labeled with the highly fluorescent fluorophore 8-aminopyrene 1,3,6-trisulfonate (APTS). By using fluorophore-assisted carbohydrate electrophoresis (FACE) on polyacrylamide gels, which utilizes equipment readily available in most molecular biology laboratories, many APTS-labeled glycans can be simultaneously resolved...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28417372/separation-and-visualization-of-glycans-by-fluorophore-assisted-carbohydrate-electrophoresis
#15
Mélissa Robb, Joanne K Hobbs, Alisdair B Boraston
Fluorophore-assisted carbohydrate electrophoresis (FACE) is a method in which a fluorophore is covalently attached to the reducing end of carbohydrates, thereby allowing visualization following high-resolution separation by electrophoresis. This method can be used for carbohydrate profiling and sequencing, as well as for the determination of the specificity of carbohydrate-active enzymes. Here, we describe and demonstrate the use of FACE to separate and visualize the glycans released following digestion of oligosaccharides by glycoside hydrolases (GHs) using two examples: (1) the digestion of chitobiose by the streptococcal β-hexosaminidase GH20C, and (2) the digestion of glycogen by the GH13 member SpuA...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28416680/using-microsecond-single-molecule-fret-to-determine-the-assembly-pathways-of-t4-ssdna-binding-protein-onto-model-dna-replication-forks
#16
Carey Phelps, Brett Israels, Davis Jose, Morgan C Marsh, Peter H von Hippel, Andrew H Marcus
DNA replication is a core biological process that occurs in prokaryotic cells at high speeds (∼1 nucleotide residue added per millisecond) and with high fidelity (fewer than one misincorporation event per 10(7) nucleotide additions). The ssDNA binding protein [gene product 32 (gp32)] of the T4 bacteriophage is a central integrating component of the replication complex that must continuously bind to and unbind from transiently exposed template strands during DNA synthesis. We here report microsecond single-molecule FRET (smFRET) measurements on Cy3/Cy5-labeled primer-template (p/t) DNA constructs in the presence of gp32...
April 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28416130/ph-dependant-fluorescence-switching-of-an-i-motif-structure-incorporating-an-isomeric-azobenzene-pyrene-fluorophore
#17
Han Na Joo, Thuy Van Thi Nguyen, Hyeon Kyeong Chae, Young Jun Seo
In this study, we synthesized an Azo-py phosphoramidite, featuring azobenzene and pyrene units, as a novel fluorescent and isomeric (trans- and cis-azobenzene units) material, which we incorporated in an i-motif DNA sequence. We then monitored the structural dynamics and changes in fluorescence as the modified DNA sequences transformed from single strands at pH 7 to i-motif quadruplex structures at pH 3. After incorporating Azo-py into the 4A loop position of an i-motif sequence, dramatic changes in fluorescence occurred as the DNA structures changed from single-strands to i-motif quadruplex structures...
April 6, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28414951/development-of-a-two-photon-fluorescent-turn-on-probe-with-far-red-emission-for-thiophenols-and-its-bioimaging-application-in-living-tissues
#18
Huiming Shang, Hua Chen, Yonghe Tang, Yanyan Ma, Weiying Lin
Thiophenol is a highly toxic compound which is essential in the field of organic synthesis and drug design. However, the accumulation of thiophenols in the environment may cause serious health problems for human bodies ultimately. Therefore, it is critical to develop efficient methods for visualization of thiophenol species in biological samples. In this work, an innovative two-photon fluorescent turn-on probe FR-TP with far-red emission for thiophenols based on FR-NH2 fluorophore and 2,4-dinitrophenylsulfonyl recognition site was reported...
April 14, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28414462/pyrene-apelin-conjugation-modulates-fluorophore-and-peptide-micelle-interactions
#19
Robin E Patterson, Nathan Weatherbee-Martin, Jan K Rainey
Bioactive apelin peptide forms ranging in length from 12 to 55 amino acids bind to and activate the apelin receptor (AR or APJ), a class A G-protein coupled receptor. Apelin-12, -17, and -36 isoforms, named according to length, with an additional N-terminal cysteine residue allowed for regiospecific and efficient conjugation of pyrene-maleimide. Through steady-state fluorescence spectroscopy, the emission properties of pyrene in aqueous buffer were compared to those of the pyrene-apelin conjugates both without and with zwitterionic or anionic micelles...
April 17, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28411805/a-ratiometric-fluorescent-probe-for-rapid-and-sensitive-detection-of-biothiols-in-fetal-bovine-serum
#20
Fengyang Wang, Chongchong Feng, Linlin Lu, Zhiai Xu, Wen Zhang
Herein, a ratiometric turn-on fluorescent probe for sensitive detection of biothiols was designed. The probe consisted of two parts: one was rhodamine B serving as a fluorescence reference, and the other was coumarin derivative as the responsive fluorophore with an acrylate group for biothiols recognition. The response was based on the mechanism of Michael addition and intramolecular cyclization reaction, and the probe showed ratiometric and sensitive response to biothiols. Especially, the detection limit of this probe for cysteine was found to be 0...
July 1, 2017: Talanta
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