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https://www.readbyqxmd.com/read/29138775/microsecond-resolved-single-molecule-fret-time-series-measurements-based-on-the-line-confocal-optical-system-combined-with-hybrid-photodetectors
#1
Hiroyuki Oikawa, Takumi Takahashi, Supawich Kamonprasertsuk, Satoshi Takahashi
Single-molecule (sm) fluorescence time series measurements based on the line confocal optical system are a powerful strategy for the investigation of the structure, dynamics, and heterogeneity of biological macromolecules. This method enables the detection of more than several thousands of fluorescence photons per millisecond from single fluorophores, implying that the potential time resolution for measurements of the fluorescence resonance energy transfer (FRET) efficiency is 10 μs. However, the necessity of using imaging photodetectors in the method limits the time resolution in the FRET efficiency measurements to approximately 100 μs...
November 15, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29137172/fluorescent-magnetopolymersomes-a-theranostic-platform-to-track-intracellular-delivery
#2
Oliver Bixner, Noga Gal, Christoph Zaba, Andrea Scheberl, Erik Reimhult
We present a potential theranostic delivery platform based on the amphiphilic diblock copolymer polybutadiene-block-poly (ethylene oxide) combining covalent fluorescent labeling and membrane incorporation of superparamagnetic iron oxide nanoparticles for multimodal imaging. A simple self-assembly and labeling approach to create the fluorescent and magnetic vesicles is described. Cell uptake of the densely PEGylated polymer vesicles could be altered by surface modifications that vary surface charge and accessibility of the membrane active species...
November 13, 2017: Materials
https://www.readbyqxmd.com/read/29136904/a-light-up-fluorescent-probe-for-citrate-detection-based-on-bispyridinum-amides-with-aggregation-induced-emission-feature
#3
Chenchen Liu, Yandi Hang, Tao Jiang, Ji Yang, Xiao Zhang, Jianli Hua
Citrate is an important intermediate in the citric acid cycle, a vital metabolic pathway for animals, plants and bacteria. It is of great significance to detect its levels in human beings because several diseases may cause the abnormal of citrate. In this paper, a new turn-on fluorescent sensor (TPE-Py) using the classic tetraphenylethylene (TPE) as the aggregation-induced emission (AIE) fluorophore and bipyridinium-based amides as the recognition receptor has been synthesized for the detection of citrate. The probe exhibits good selectivity and sensitivity to citrate with a relatively low detection limit (1...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136833/a-cell-penetrating-ratiometric-probe-for-simultaneous-measurement-of-lysosomal-and-cytosolic-ph-change
#4
Meng-Chan Xia, Lesi Cai, Sichun Zhang, Xinrong Zhang
A new ratiometric fluorescent probe based on cell-penetrating peptides (CPPs) was constructed for whole-cell pH mapping and simultaneous measurement of pH changes in the cytoplasm and lysosomes. The arginine-rich CPP, R12K worked as linker, carrier and part of the fluorophore. Benefiting from R12K, the fluorescent probe is completely water soluble, membrane permeable and well biocompatible. It shows high selectivity, sensitivity and reversibility to pH fluctuations. The ratio of fluorescence intensities F519/F582 increased from 0...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136298/tuning-the-f%C3%A3-rster-resonance-energy-transfer-through-self-assembly-approach-for-efficient-white-light-emission-in-aqueous-medium
#5
Pragyan Pallavi, Bahadur Sk, Palak Ahir, Abhijit Patra
A simple and cost-effective methodology employing environmentally benign substances for the fabrication of white-light emitting materials is important for practical applications in the field of lighting and display devices. Designing of purely organic-based white-light emitting system with high quantum efficiency in the aqueous medium is an unmet challenge. With this objective, a new class of pyridoindole based hydrophobic fluorophore 6,7,8,9-tetrapropylpyrido[1,2-a]indole-10-carbaldehye (TPIC) was introduced...
November 14, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29136199/site-specific-dual-color-labeling-of-long-rnas-for-single-molecule-spectroscopy
#6
Meng Zhao, Fabio D Steffen, Richard Börner, Michelle F Schaffer, Roland K O Sigel, Eva Freisinger
Labeling of long RNA molecules in a site-specific yet generally applicable manner is integral to many spectroscopic applications. Here we present a novel covalent labeling approach that is site-specific and scalable to long intricately folded RNAs. In this approach, a custom-designed DNA strand that hybridizes to the RNA guides a reactive group to target a preselected adenine residue. The functionalized nucleotide along with the concomitantly oxidized 3'-terminus can subsequently be conjugated to two different fluorophores via bio-orthogonal chemistry...
November 9, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29134803/a-fluorescent-binary-ensemble-based-on-pyrene-derivative-and-sds-assemblies-as-a-chemical-tongue-for-discriminating-metal-ions-and-brand-water
#7
Lijun Zhang, Xinyan Huang, Yuan Cao, Yunhong Xin, Liping Ding
Enormous effort has been put to the detection and recognition of various heavy metal ions due to their involvement in serious environmental pollution and many major diseases. The present work developed a single fluorescent sensor ensemble that can distinguish and identify a variety of heavy metal ions. A pyrene-based fluorophore (PB) containing a metal ion receptor group was specially designed and synthesized. Anionic surfactant SDS assemblies can effectively adjust its fluorescence behavior. The selected binary ensemble based on PB/SDS assemblies can exhibit multiple emission bands and provide wavelength-based cross-reactive responses to a series of metal ions to realize pattern recognition ability...
November 14, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29134784/a-general-strategy-for-development-of-near-infrared-fluorescent-probes-for-bioimaging
#8
Ming Xian, Wei Chen, Shi Xu, Jacob J Day, Difei Wang
Near-infrared (NIR) fluorescent dyes with favorable photophysical properties are highly useful for bioimaging, but such dyes are still rare. Here, we describe the development of a unique class of NIR dyes via modifying the rhodol-scaffold with fused tetrahydroquinoxaline rings. These new dyes showed large Stokes shifts (>110 nm). Among them, WR3, WR4, WR5 and WR6 displayed high fluorescence quantum yields and excellent photostability in aqueous solutions. Moreover, their fluorescence properties were tunable by easy modifications on the phenolic hydroxyl group...
November 13, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29131957/excimer-formation-dynamics-of-dipyrenyldecane-in-structurally-different-ionic-liquids
#9
Anita Yadav, Siddharth Pandey
Ionic liquids, being composed of ions alone, may offer alternate pathways for molecular aggregation. These pathways could be controlled by the chemical structure of the cation and the anion of the ionic liquids. Intramolecular excimer formation dynamics of a bifluorophoric probe, 1,3-bis(1-pyrenyl)decane [1Py(10)1Py], where the fluorophoric pyrene moieties are separated by a long decyl chain, is investigated in seven different ionic liquids in 10 - 90 °C temperature range. The long alkyl separator allows for ample interaction with the solubilizing milieu prior to the formation of the excimer...
November 13, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29131610/real-time-imaging-of-single-molecule-enzyme-cascade-using-a-dna-origami-raft
#10
Lele Sun, Yanjing Gao, Yan Xu, Jie Chao, Huajie Liu, Lianhui Wang, Di Li, Chunhai Fan
The dynamics of enzymes are directly associated with their functions in various biological processes. Nevertheless, the ability to image motions of single enzymes in a highly parallel fashion remains a challenge. Here, we develop a DNA origami raft-based platform for in-situ real-time imaging of enzyme cascade at the single-molecule level. The motions of enzymes are rationally controlled via different tethering modes on a two-dimensional (2D) supported lipid bilayer (SLB). We construct an enzyme cascade by anchoring catalase on cholesterol-labeled double stranded (ds) DNA, and glucose oxidase on cholesterol-labeled origami rafts...
November 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29131578/optically-detected-magnetic-resonance-for-selective-imaging-of-diamond-nanoparticles
#11
Margaret E Robinson, James D Ng, Huilong Zhang, Joseph T Buchman, Olga A Shenderova, Christy L Haynes, Zhenqiang Ma, Randall H Goldsmith, Robert J Hamers
While there is great interest in understanding the fate and transport of nanomaterials in the environment and in biological systems, the detection of nanomaterials in complex matrices by fluorescence methods is complicated by photodegradation, blinking, and by the presence of natural organic material and other fluorescent background signals that hamper detection of fluorescent nanomaterials of interest. Optically detected magnetic resonance (ODMR) of nitrogen-vacancy (NV) centers in diamond nanoparticles provides a pathway toward background-free fluorescence measurements, as the application of a resonant microwave field can selectively modulate the intensity from NV centers in the diamond in nanodiamonds of various diameters in complex materials systems using on-resonance and off-resonance microwave fields...
November 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29131088/development-and-evaluation-of-lipid-nanoparticles-containing-natural-botanical-oil-for-sun-protection-characterization-and-in-vitro-and-in-vivo-human-skin-permeation-and-toxicity
#12
Newton Andréo-Filho, Antonio Vinicius Kosiski Bim, Telma Mary Kaneko, Nidia Ayumi Kitice, Isha N Haridass, Eman Abd, Patricia Santos Lopes, Sachin S Thakur, Harendra S Parekh, Michael S Roberts, Jeffrey E Grice, Heather A E Benson, Vânia Rodrigues Leite-Silva
The use of sunscreen products is widely promoted by schools, government agencies, and health-related organizations to minimize sunburn and skin damage. In this study, we developed stable solid lipid nanoparticles (SLNs) containing the chemical UV filter octyl methoxycinnamate (OMC). In parallel, we produced similar stable SLNs in which 20% of the OMC content was replaced by the botanical urucum oil. When these SLNs were applied to the skin of human volunteers, no changes in fluorescence lifetimes or redox ratios of the endogenous skin fluorophores were seen, suggesting that the formulations did not induce toxic responses in the skin...
November 4, 2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/29130776/new-insights-into-effects-of-aromatic-amino-acids-on-hydroxyapatite
#13
Y R Guo, X Yang, X W Feng, Y Sa, M Wang, P Li, T Jiang
Biomimetics inspired by superstructures and extraordinary properties of teeth have resulted in tooth repair and the generation of novel materials. However, little attention has been paid to tooth color, whose origin remains unknown. Based on recent studies, fluorophores-mainly aromatic amino acids (AAAs) in proteins-might be responsible for tooth color. We synthesized carbonated hydroxyapatite (HA; the mineral phase of teeth) in the presence of different amino acids (AAs; the basic units of protein matrix of teeth) as a simplified model of teeth to explore the color source at the AA level...
November 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29130205/visualizing-rna-in-live-bacterial-cells-using-fluorophore-and-quencher-binding-aptamers
#14
Murat Sunbul, Ankita Arora, Andres Jäschke
To elucidate the roles, dynamics, and regulation of RNAs, it is vital to be able to visualize the RNA of interest (ROI) in living cells noninvasively. Here, we describe a novel live-cell RNA imaging method using fluorophore- and quencher-binding aptamers, which can be genetically fused to the ROI. In this method, new membrane permeable and nonfluorescent fluorophore-quencher conjugates were utilized, and we showed that their fluorescence increases dramatically upon binding to fluorophore- or quencher-binding aptamers...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29128399/nitric-oxide-production-by-glomerular-podocytes
#15
Oleg Palygin, Daria V Ilatovskaya, Vladislav Levchenko, Bradley T Endres, Aron M Geurts, Alexander Staruschenko
Nitric Oxide (NO), a potent vasodilator and vital signaling molecule, has been shown to contribute to the regulation of glomerular ultrafiltration. However, whether changes in NO occur in podocytes during the pathogenesis of salt-sensitive hypertension has not yet been thoroughly examined. We showed here that podocytes produce NO, and further hypothesized that hypertensive animals would exhibit reduced NO production in these cells in response to various paracrine factors, which might contribute to the damage of glomeruli filtration barrier and development of proteinuria...
November 8, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29128078/applications-of-mass-cytometry-in-clinical-medicine-the-promise-and-perils-of-clinical-cytof
#16
REVIEW
Gregory K Behbehani
Mass cytometry is a novel technology similar to flow cytometry in which antibodies are tagged with heavy metal molecules rather than fluorophores and then detected with time-of-flight mass spectrometry. This enables measurement of up to 50 simultaneous parameters with no autofluorescent background and little or no spillover or required compensation. Mass cytometry has tremendous potential for the analysis of highly complex clinical samples for the diagnosis and monitoring of malignant and autoimmune disorders...
December 2017: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/29127798/characterization-and-sources-of-colored-dissolved-organic-matter-in-a-coral-reef-ecosystem-subject-to-ultramafic-erosion-pressure-new-caledonia-southwest-pacific
#17
Chloé Martias, Marc Tedetti, François Lantoine, Léocadie Jamet, Cécile Dupouy
The eastern lagoon of New Caledonia (NC, Southwest Pacific), listed as a UNESCO World Heritage site, hosts the world's second longest double-barrier coral reef. This lagoon receives river inputs, oceanic water arrivals, and erosion pressure from ultramafic rocks, enriched in nickel (Ni) and cobalt (Co). The aim of this study was to characterize colored dissolved organic matter (CDOM), as well as to determine its main sources and its possible relationships (through the use of Pearson correlation coefficients, r) with biogeochemical parameters, plankton communities and trace metals in the NC eastern lagoon...
November 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29123102/semisynthetic-fluorescent-ph-sensors-for-imaging-exocytosis-and-endocytosis
#18
Magalie Martineau, Agila Somasundaram, Jonathan B Grimm, Todd D Gruber, Daniel Choquet, Justin W Taraska, Luke D Lavis, David Perrais
The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission...
November 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/29121607/preparation-of-fluorescently-labeled-silica-nanoparticles-using-an-amino-acid-catalyzed-seeds-regrowth-technique-application-to-latent-fingerprints-detection-and-hemocompatibility-studies
#19
Walid M Abdelwahab, Edjohnier Phillips, Gabor Patonay
The efficiency of an amino acid catalyzed seed regrowth technique (ACSRT) in synthesizing twelve fluorescently labeled core-shell silica nanoparticles (FLSNPs) with tunable sizes, tailored hydrophobicity, low polydispersity as well as high labeling efficiency and minimized dye leakage using different combinations of organosilicate monomers and fluorophores have been systematically investigated in this report. The utilization of some of these FLSNPs in some applications that are facilitated by hydrophobicity such as developing and visualizing latent fingerprints (LFPs) on different surfaces was also investigated...
October 17, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29120485/measuring-ph-of-the-coxiella-burnetii-parasitophorous-vacuole
#20
Dhritiman Samanta, Stacey D Gilk
Coxiella burnetii is the causative agent of human Q fever, a zoonotic disease that can cause a debilitating, flu-like illness in acute cases, or a life-threatening endocarditis in chronic patients. An obligate intracellular bacterial pathogen, Coxiella survives and multiplies in a large lysosome-like vacuole known as the Coxiella parasitophorous vacuole (CPV). A unique characteristic of the CPV is the acidic environment (pH ∼5.0), which is required to activate Coxiella metabolism and the Coxiella type 4 secretion system (T4SS), a major virulence factor required for intracellular survival...
November 9, 2017: Current Protocols in Microbiology
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