keyword
MENU ▼
Read by QxMD icon Read
search

Protein aggregation

keyword
https://www.readbyqxmd.com/read/29679468/high-efficiency-affinity-precipitation-of-multiple-industrial-mabs-and-fc-fusion-proteins-from-cell-culture-harvests-using-z-elp-e2-nanocages
#1
Andrew R Swartz, Xuankuo Xu, Steven J Traylor, Zheng Jian Li, Wilfred Chen
Affinity precipitation using Z-ELP-E2 nanocages has been shown to be a promising alternative to Protein A chromatography for monoclonal antibody (mAb) purification. We have previously described a high-yielding, affinity precipitation process capable of rapidly capturing mAbs from cell culture through spontaneous, multi-valent crosslinking into large aggregates. To challenge the capabilities of this technology, nanocage affinity precipitation was investigated using four industrial mAbs (mAbs A-D) and one Fc fusion protein (Fc A) with diverse molecular properties...
April 21, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29679389/down-regulation-of-nramp1-is-associated-with-mptp-mpp-induced-%C3%AE-synuclein-accumulation-and-neurotoxicity
#2
Kuo-Chen Wu, Horng-Huei Liou, Chih-Yu Lee, Chun-Jung Lin
AIMS: The accumulation of α-synuclein is a hallmark in the pathogenesis of Parkinson's disease (PD). Natural resistance-associated macrophage protein-1 (Nramp1) was previously shown to contribute to the degradation of extracellular α-synuclein in microglia under conditions of iron overload. This study was aimed at investigating the role of Nramp1 in α-synuclein pathology in the neuron under MPTP/MPP+ treatment. METHODS: The expression of Nramp1 and pathological features (including iron and α-synuclein accumulation) were examined in the dopaminergic neurons of humans (with and without PD) and of mice (with and without receiving chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intoxication)...
April 21, 2018: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29678763/construction-of-quenchbodies-to-detect-and-image-amyloid-%C3%AE-oligomers
#3
Jinhua Dong, Richi Fujita, Tamotsu Zako, Hiroshi Ueda
A quenchbody (Q-body) is an antibody-based biosensor that employs fluorescence quenching of the dye(s) attached to the antibody fragment, which are de-quenched upon antigen binding. In this study, we aimed to develop Fab type Q-bodies (UQ-bodies) to aid the diagnosis of Alzheimer's disease (AD). Characteristic senile plaques in AD consist of amyloid-β peptide (Aβ) generated from the amyloid precursor protein. Aβ42, one of the major peptide forms, aggregates fast and manifests higher neurotoxicity. Recent studies showed that Aβ oligomers, such as Aβ-derived diffusible ligand (ADDL), are more toxic than fibrils...
April 17, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29678468/the-s-layer-biogenesis-system-of-synechocystis-6803-role-of-sll1180-and-sll1181-e-coli-hlyb-and-hlyd-analogs-as-type-i-secretion-components-for-sll1951-export
#4
Rachna Agarwal, Julian P Whitelegge, Sanjay Saini, Amit Prakash Shrivastav
Multiple secretion pathways are known for export of protein(s) forming the S-layer in bacteria. The unicellular model cyanobacterium Synechocystis sp. strain PCC 6803 (hereafter S. 6803) also possesses a well-defined S-layer composed of Sll1951 protein. However, the mechanism of its secretion is not completely understood. In the present study, the putative T1SS (Type I secretion system) components, Sll1180 and Sll1181 [inner membrane ABC transporter and membrane fusion protein (MFP), respectively] were characterized for their role in Sll1951 secretion...
April 18, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29677544/peptide-based-therapeutics-and-their-use-for-the-treatment-of-neurodegenerative-and-other-diseases
#5
REVIEW
Mohammad Hassan Baig, Khurshid Ahmad, Mohd Saeed, Ahmed M Alharbi, George E Barreto, Ghulam Md Ashraf, Inho Choi
Bioactive peptides are actively involved in different biological functions and importantly contribute to human health, and the use of peptides as therapeutics has a long successful history in disease management. A number of peptides have wide-ranging therapeutic effects, such as antioxidant, antimicrobial, and antithrombotic effects. Neurodegenerative diseases are typically caused by abnormal aggregations of proteins or peptides, and the depositions of these aggregates in or on neurons, disrupt signaling and eventually kill neurons...
April 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29677492/substrate-insolubility-dictates-hsp104-dependent-endoplasmic-reticulum-associated-degradation
#6
G Michael Preston, Christopher J Guerriero, Meredith B Metzger, Susan Michaelis, Jeffrey L Brodsky
Misfolded proteins in the endoplasmic reticulum (ER) are destroyed by ER-associated degradation (ERAD). Although the retrotranslocation of misfolded proteins from the ER has been reconstituted, how a polypeptide is initially selected for ERAD remains poorly defined. To address this question while controlling for the diverse nature of ERAD substrates, we constructed a series of truncations in a single ER-tethered domain. We observed that the truncated proteins exhibited variable degradation rates and discovered a positive correlation between ERAD substrate instability and detergent insolubility, which demonstrates that aggregation-prone species can be selected for ERAD...
April 19, 2018: Molecular Cell
https://www.readbyqxmd.com/read/29676852/asp-58-modulates-lens-%C3%AE-a-crystallin-oligomer-formation-and-chaperone-function
#7
Takumi Takata, Tooru Nakamura-Hirota, Rintaro Inoue, Ken Morishima, Nobuhiro Sato, Masaaki Sugiyama, Noriko Fujii
Senile cataract onset is caused by insolubilization of lens proteins. The lens crystallin protein family correctly orders the formation of homo- or hetero-oligomers in lens fiber cells. Because lens fiber cells do not divide, covalent post-translational modifications such as isomerization of aspartate residues, accumulate with aging. Although many isomerization sites of αA-crystallin have been reported, their structural and functional contributions have never been identified. In this study, αA-crystallin was extracted from the aged human lens and separated into each oligomeric state by size exclusion chromatography and electrophoresis...
April 20, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29676481/high-mobility-group-box-1-in-parkinson-s-disease-from-pathogenesis-to-therapeutic-approaches
#8
REVIEW
Efthalia Angelopoulou, Christina Piperi, Athanasios G Papavassiliou
Parkinson's disease (PD) presents the second most common neurodegenerative disorder with largely unknown pathogenesis and inefficient therapeutic management. Accumulating data indicate that neuroinflammation, autophagy impairment, α-synuclein aggregation and mitochondrial dysfunction may contribute to PD onset; however the molecular mechanisms underlying these pathophysiological processes are still under elucidation. Interestingly, recent evidence has indicated that High-mobility group box 1 (HMGB1), a DNA-binding protein that can be actively secreted by inflammatory cells and passively released by necrotic cells may play a key role in PD pathogenesis...
April 20, 2018: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29676228/glaucoma-pathogenesis-and-neurotrophins-focus-on-the-molecular-and-genetic-basis-for-therapeutic-prospects
#9
Nitin Chitranshi, Yogita Dheer, Mojdeh Abbasi, Yuyi You, Stuart L Graham, Vivek Gupta
BACKGROUND: Retinal ganglion cell (RGC) degeneration is a major feature of glaucoma pathology. Neuroprotective approaches that delay or halt the progression of RGC loss are needed to prevent vision loss which can occur even after conventional medical or surgical treatments to lower intraocular pressure. OBJECTIVE: The aim of this review was to examine the progress in genetics and cellular mechanisms associated with endoplasmic reticulum (ER) stress, RGC dysfunction and cell death pathways in glaucoma...
April 19, 2018: Current Neuropharmacology
https://www.readbyqxmd.com/read/29675368/mincle-in-the-innate-immune-response-of-mice-fungal-keratitis
#10
Guo-Rong Yu, Jing Lin, Jie Zhang, Cheng-Ye Che, Xu-Dong Peng, Cui Li, Li-Ting Hu, Guo-Qiang Zhu, Kun He, Gui-Qiu Zhao
AIM: To investigate how macrophage inducible C-type lectin (Mincle) influences inflammation in mice fungal keratitis induced by Aspergillus fumigatus ( A. fumigatus ). METHODS: C57BL/6 mice were infected with A. fumigatus after pretreated with Mincle agonist TDB or Mincle neutralizing antibody (MincleAb), taking DMSO or IgG as control group respectively. The cornea lesions were monitored with slit-lamp microscope and evaluated by clinical score. Mincle expression was assessed using reverse transcription-ploymerase chain reaction (RT-PCR) and immunostaining...
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29675133/benefit-of-oleuropein-aglycone-for-alzheimer-s-disease-by-promoting-autophagy
#11
REVIEW
Joaquín G Cordero, Ramón García-Escudero, Jesús Avila, Ricardo Gargini, Vega García-Escudero
Alzheimer's disease is a proteinopathy characterized by accumulation of hyperphosphorylated Tau and β -amyloid. Autophagy is a physiological process by which aggregated proteins and damaged organelles are eliminated through lysosomal digestion. Autophagy deficiency has been demonstrated in Alzheimer's patients impairing effective elimination of aggregates and damaged mitochondria, leading to their accumulation, increasing their toxicity and oxidative stress. In the present study, we demonstrated by microarray analysis the downregulation of fundamental autophagy and mitophagy pathways in Alzheimer's patients...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29674518/interrogating-the-functions-of-prdm9-domains-in-meiosis
#12
Sarah Thibault-Sennett, Qi Yu, Fatima Smagulova, Jeff Cloutier, Kevin Brick, R Daniel Camerini-Otero, Galina V Petukhova
Homologous recombination is required for proper segregation of homologous chromosomes during meiosis. It predominantly occurs at recombination hotspots that are defined by the DNA binding specificity of the PRDM9 protein. PRDM9 contains three conserved domains typically involved in regulation of transcription, yet, the role of PRDM9 in gene expression control is not clear. Here we analyze the germline transcriptome of Prdm9-/- male mice in comparison to Prdm9+/+ males and find no apparent differences in the mRNA and miRNA profiles...
April 19, 2018: Genetics
https://www.readbyqxmd.com/read/29674495/platelet-munc13-4-regulates-hemostasis-thrombosis-and-airway-inflammation
#13
Eduardo I Cardenas, Keegan Breaux, Qi Da, Jose R Flores, Marco A Ramos, Michael J Tuvim, Alan R Burns, Rolando E Rumbaut, Roberto Adachi
Platelet degranulation is crucial for hemostasis and may participate in inflammation. Exocytosis in platelets is mediated by SNARE proteins and should be controlled by Munc13 proteins. We found that platelets express Munc13-2 and -4. We assessed platelet granule exocytosis in Munc13-2 and -4 global and conditional KO mice, and observed that deletion of Munc13-4 ablates dense granule release and indirectly impairs alpha granule exocytosis. We found no exocytic role for Munc13-2 in platelets, not even in the absence of Munc13-4...
April 19, 2018: Haematologica
https://www.readbyqxmd.com/read/29673913/epothilone-d-inhibits-microglia-mediated-spread-of-alpha-synuclein-aggregates
#14
Dario Valdinocci, Gary Grant, Tracey Dickson, Dean L Pountney
Multiple System Atrophy (MSA) is a progressive neurodegenerative disease characterized by chronic neuroinflammation and widespread α-synuclein (α-syn) cytoplasmic inclusions. Neuroinflammation associated with microglial cells is typically located in brain regions with α-syn deposits. The potential link between microglial cell migration and the transport of pathological α-syn protein in MSA was investigated. Qualitative analysis via immunofluorescence of MSA cases (n = 4) revealed microglial cells bearing α-syn inclusions distal from oligodendrocytes bearing α-syn cytoplasmic inclusions, as well as close interactions between microglia and oligodendrocytes bearing α-syn, suggestive of a potential transfer mechanism between microglia and α-syn bearing cells in MSA and the possibility of microglia acting as a mobile vehicle to spread α-syn between anatomically connected brain regions...
April 16, 2018: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/29673839/development-of-a-macrophage-targeting-and-phagocytosis-inducing-bio-nanocapsule-based-nanocarrier-for-drug-delivery
#15
Hao Li, Kenji Tatematsu, Masaharu Somiya, Masumi Iijima, Shun' Ichi Kuroda
Macrophage hyperfunction or dysfunction is tightly associated with various diseases, such as osteoporosis, inflammatory disorder, and cancers. However, nearly all conventional drug delivery system (DDS) nanocarriers utilize endocytosis for entering target cells; thus, the development of macrophage-targeting and phagocytosis-inducing DDS nanocarriers for treating these diseases is required. In this study, we developed a hepatitis B virus (HBV) envelope L particle (i.e., bio-nanocapsule (BNC)) outwardly displaying a tandem form of protein G-derived IgG Fc-binding domain and protein L-derived IgG Fab-binding domain (GL-BNC)...
April 16, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29673549/tau-and-neuroinflammation-what-impact-for-alzheimer-s-disease-and-tauopathies
#16
REVIEW
Cyril Laurent, Luc Buée, David Blum
Alzheimer's Disease (AD) is a chronic neurodegenerative disorder and the most common type of dementia (60-80% of cases). In 2016, nearly 44 million people were affected by AD or related dementia. AD is characterized by progressive neuronal damages leading to subtle and latter obvious decline in cognitive functions including symptoms such as memory loss or confusion, which ultimately require full-time medical care. Its neuropathology is defined by the extracellular accumulation of amyloid-β (Aβ) peptide into amyloid plaques, and intraneuronal neurofibrillary tangles (NFT) consisting of aggregated hyper- and abnormal phosphorylation of tau protein...
February 2018: Biomedical Journal
https://www.readbyqxmd.com/read/29673487/a-novel-akt-activator-sc79-prevents-acute-hepatic-failure-induced-by-fas-mediated-apoptosis-of-hepatocytes
#17
Wei Liu, Zhen-Tang Jing, Shu-Xiang Wu, Yun He, Yan-Ting Lin, Wan-Nan Chen, Xin-Jian Lin, Xu Lin
Acute liver failure is a serious clinical problem of which the underlying pathogenesis remains unclear and for which effective therapies are lacking. The Fas receptor/ligand system, which is negatively regulated by AKT, is known to play a prominent role in hepatocytic cell death. We hypothesized that AKT activation may represent a strategy to alleviate Fas-induced fulminant liver failure. We report here that a novel AKT activator, SC79, protects hepatocytes from apoptosis induced by agonistic anti-Fas antibody CH11 (for humans) or Jo2 (for mice) and significantly prolongs the survival of mice given a lethal dose of Jo2...
May 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29673239/effects-of-treadmill-exercise-on-pi3k-akt-gsk-3%C3%AE-pathway-and-tau-protein-in-high-fat-diet-fed-rats
#18
Jae-Hoon Jeong, Eun-Bum Kang
PURPOSE: This study aimed to clearly evaluate the effects of obesity on cerebral health. Thus, we induced obesity in rats using a long-term high-fat diet (HFD), then investigated its effects on insulin signaling and tau hyperphosphorylation. Additionally, we examined the effects of 8 weeks of treadmill exercise (TE) on insulin signaling and tau hyperphosphorylation. METHODS: Rats were separated into Normal Diet-Control, HFD-Control, and HFD-TE groups. TE loads were gradually increased...
March 30, 2018: Journal of Exercise Nutrition & Biochemistry
https://www.readbyqxmd.com/read/29673150/immunohistochemical-evidence-from-app-transgenic-mice-for-glutaminyl-cyclase-as-drug-target-to-diminish-pe-abeta-formation
#19
Maike Hartlage-Rübsamen, Alexandra Bluhm, Anke Piechotta, Miriam Linnert, Jens-Ulrich Rahfeld, Hans-Ulrich Demuth, Inge Lues, Peer-Hendrik Kuhn, Stefan F Lichtenthaler, Steffen Roßner, Corinna Höfling
Oligomeric assemblies of neurotoxic amyloid beta (Abeta) peptides generated by proteolytical processing of the amyloid precursor protein (APP) play a key role in the pathogenesis of Alzheimer’s disease (AD). In recent years, a substantial heterogeneity of Abeta peptides with distinct biophysical and cell biological properties has been demonstrated. Among these, a particularly neurotoxic and disease-specific Abeta variant is N-terminally truncated and modified to pyroglutamate (pE-Abeta). Cell biological and animal experimental studies imply the catalysis of this modification by the enzyme glutaminyl cyclase (QC)...
April 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29672841/mutant-myo1f-alters-the-mitochondrial-network-and-induces-tumor-proliferation-in-thyroid-cancer
#20
Chiara Diquigiovanni, Christian Bergamini, Cecilia Evangelisti, Federica Isidori, Andrea Vettori, Natascia Tiso, Francesco Argenton, Anna Costanzini, Luisa Iommarini, Hima Anbunathan, Uberto Pagotto, Andrea Repaci, Giulia Babbi, Rita Casadio, Giorgio Lenaz, Kerry J Rhoden, Anna Maria Porcelli, Romana Fato, Anne Bowcock, Marco Seri, Giovanni Romeo, Elena Bonora
Familial aggregation is a significant risk factor for the development of thyroid cancer and Familial Non-Medullary Thyroid Cancer (FNMTC) accounts for 5-7% of all NMTC. Whole Exome Sequencing analysis in the family affected by FNMTC with oncocytic features where our group previously identified a predisposing locus on chromosome 19p13.2, revealed a novel heterozygous mutation (c.400G>A, NM_012335; p.Gly134Ser) in exon 5 of MYO1F, mapping to the linkage locus. In the thyroid FRTL-5 cell model stably expressing the mutant MYO1F p...
April 19, 2018: International Journal of Cancer. Journal International du Cancer
keyword
keyword
56129
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"