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https://www.readbyqxmd.com/read/29778563/development-and-evaluation-of-a-rapid-nucleic-acid-amplification-method-to-detect-influenza-a-and-b-viruses-in-human-respiratory-specimens
#1
Sonja Elf, Pauliina Auvinen, Lisa Jahn, Karoliina Liikonen, Solveig Sjöblom, Päivi Saavalainen, Minna Mäki, Kevin E Eboigbodin
Isothermal nucleic acid amplification methods can potentially shorten the amount of time required to diagnose influenza. We developed and evaluated a novel isothermal nucleic acid amplification method, RT-SIBA to rapidly detect and differentiate between influenza A and B viruses in a single reaction tube. The performance of the RT-SIBA Influenza assay was compared with two established RT-PCR methods. The sensitivities of the RT-SIBA, RealStar RT-PCR, and CDC RT-PCR assays for the detection of influenza A and B viruses in the clinical specimens were 98...
April 18, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29778498/validation-on-milk-and-sprouts-of-en-iso-16654-2001-microbiology-of-food-and-animal-feeding-stuffs-horizontal-method-for-the-detection-of-escherichia-coli-o157
#2
Rosangela Tozzoli, Antonella Maugliani, Valeria Michelacci, Fabio Minelli, Alfredo Caprioli, Stefano Morabito
In 2006, the European Committee for standardisation (CEN)/Technical Committee 275 - Food analysis - Horizontal methods/Working Group 6 - Microbiology of the food chain (TC275/WG6), launched the project of validating the method ISO 16654:2001 for the detection of Escherichia coli O157 in foodstuff by the evaluation of its performance, in terms of sensitivity and specificity, through collaborative studies. Previously, a validation study had been conducted to assess the performance of the Method No 164 developed by the Nordic Committee for Food Analysis (NMKL), which aims at detecting E...
May 8, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29778447/analysis-of-hydrophobically-modified-ethylene-oxide-urethane-rheology-modifiers-by-comprehensive-two-dimensional-liquid-chromatography
#3
Peilin Yang, Lu Bai, Wenqin Wang, John Rabasco
Hydrophobically modified ethylene oxide urethane (HEUR) rheology modifiers are polymers used widely in waterborne coatings and other consumer products. The incorporation of hydrophobic end-groups significantly affects the thickening performance of HEURs. Therefore, understanding the degree of incorporation of hydrophobic end-groups on the polymer chain can help gain important insights on the structure/property relationship for HEUR rheology modifiers. A comprehensive two-dimensional liquid chromatography (2D-LC) method was developed for separating polymeric HEUR rheology modifiers based on the number of hydrophobic end-groups incorporated on the polymer chain...
May 16, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29777985/liquid-chromatography-tandem-mass-spectrometry-for-the-quantification-of-moxifloxacin-ciprofloxacin-daptomycin-caspofungin-and-isavuconazole-in-human-plasma
#4
Julian Hösl, André Gessner, Nahed El-Najjar
A simple and precise ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous analysis of five anti-infective agents used to treat severe infections [three antibiotics (daptomycin, moxifloxacin, ciprofloxacin) and two antifungals (isavuconazole, caspofungin)] in human plasma. Sample preparation was based on protein precipitation with ice cold methanol. All five agents were analyzed with the corresponding isotopically labeled internal standards. All analytes were detected in multiple reactions monitoring (MRM) using API 4000 triple-quadrupole mass spectrometer with electrospray (ESI) source operating in positive mode...
May 12, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29777822/effects-of-global-signal-regression-and-subtraction-methods-on-resting-state-functional-connectivity-using-arterial-spin-labeling-data
#5
João Paulo Santos Silva, Luciana da Mata Mônaco, André Monteiro Paschoal, Ícaro Agenor Ferreira de Oliveira, Renata Ferranti Leoni
BACKGROUND: Arterial spin labeling (ASL) is an established magnetic resonance imaging (MRI) technique that is finding broader applications in functional studies of the healthy and diseased brain. To promote improvement in cerebral blood flow (CBF) signal specificity, many algorithms and imaging procedures, such as subtraction methods, were proposed to eliminate or, at least, minimize noise sources. Therefore, this study addressed the main considerations of how CBF functional connectivity (FC) is changed, regarding resting brain network (RBN) identification and correlations between regions of interest (ROI), by different subtraction methods and removal of residual motion artifacts and global signal fluctuations (RMAGSF)...
May 16, 2018: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/29777650/modeling-dengue-vector-population-using-remotely-sensed-data-and-machine-learning
#6
Juan M Scavuzzo, Francisco Trucco, Manuel Espinosa, Carolina B Tauro, Marcelo Abril, Carlos M Scavuzzo, Alejandro C Frery
Mosquitoes are vectors of many human diseases. In particular, Aedes ægypti (Linnaeus) is the main vector for Chikungunya, Dengue, and Zika viruses in Latin America and it represents a global threat. Public health policies that aim at combating this vector require dependable and timely information, which is usually expensive to obtain with field campaigns. For this reason, several efforts have been done to use remote sensing due to its reduced cost. The present work includes the temporal modeling of the oviposition activity (measured weekly on 50 ovitraps in a north Argentinean city) of Aedes ægypti (Linnaeus), based on time series of data extracted from operational earth observation satellite images...
May 16, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29777386/substrate-metabolism-during-ironman-triathlon-different-horses-on-the-same-courses
#7
Ed Maunder, Andrew E Kilding, Daniel J Plews
Ironman triathlons are ultra-endurance events of extreme duration. The performance level of those competing varies dramatically, with elite competitors finishing in ~ 8:00:00, and lower performing amateurs finishing in ~ 14-15:00:00. When applying appropriate values for swimming, cycling and running economies to these performance times, it is demonstrated that the absolute energy cost of these events is high, and the rate of energy expenditure increases in proportion with the athlete's competitive level...
May 18, 2018: Sports Medicine
https://www.readbyqxmd.com/read/29776531/an-evaluation-of-a-college-campus-emergency-preparedness-intervention
#8
Christofer Skurka, Brian L Quick, Tobias Reynolds-Tylus, Todd Short, Ann L Bryan
INTRODUCTION: Given the range of emergencies that beset postsecondary institutions, university administrators must take a multimodal approach to prepare campus stakeholders for safety threats. One such strategy is emergency preparedness communication. METHODS: In the present investigation, we tested the efficacy of a professionally produced video that uses the federally endorsed slogan, Run-Hide-Fight(r). Undergraduate students participated in a quasi-experiment with a pretest-posttest-delayed posttest control group design...
June 2018: Journal of Safety Research
https://www.readbyqxmd.com/read/29776097/modeling-flow-and-transport-in-fracture-networks-using-graphs
#9
S Karra, D O'Malley, J D Hyman, H S Viswanathan, G Srinivasan
Fractures form the main pathways for flow in the subsurface within low-permeability rock. For this reason, accurately predicting flow and transport in fractured systems is vital for improving the performance of subsurface applications. Fracture sizes in these systems can range from millimeters to kilometers. Although modeling flow and transport using the discrete fracture network (DFN) approach is known to be more accurate due to incorporation of the detailed fracture network structure over continuum-based methods, capturing the flow and transport in such a wide range of scales is still computationally intractable...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29776055/optimal-methylation-noise-for-best-chemotactic-performance-of-e-coli
#10
Subrata Dev, Sakuntala Chatterjee
In response to a concentration gradient of chemoattractant, E. coli bacterium modulates the rotational bias of flagellar motors which control its run-and-tumble motion, to migrate towards regions of high chemoattractant concentration. Presence of stochastic noise in the biochemical pathway of the cell has important consequences on the switching mechanism of motor bias, which in turn affects the runs and tumbles of the cell in a significant way. We model the intracellular reaction network in terms of coupled time evolution of three stochastic variables-kinase activity, methylation level, and CheY-P protein level-and study the effect of methylation noise on the chemotactic performance of the cell...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29776014/association-of-objectively-measured-physical-activity-with-brain-structure-uk-biobank-study
#11
Mark Hamer, Nikhil Sharma, G David Batty
BACKGROUND: Physical activity may be beneficial for cognition but mechanisms are unclear. We examined the association between objectively assessed physical activity and brain volume, with a focus on the hippocampus region. METHODS: We used data from UK Biobank (n=5,272; aged 55.4±7.5 yrs; 45.6% men) collected through 2013-2016. Participants wore the Axivity AX3 wrist-worn triaxial accelerometer for seven days to assess habitual physical activity. Structural magnetic resonance imaging was performed using a standard Siemens Skyra 3T running VD13A SP4 to obtain images of the brain...
May 18, 2018: Journal of Internal Medicine
https://www.readbyqxmd.com/read/29775989/higher-accuracy-of-the-lactate-minimum-test-compared-to-established-threshold-concepts-to-determine-maximal-lactate-steady-state-in-running
#12
Patrick Wahl, Lukas Zwingmann, Christian Manunzio, Jacob Wolf, Wilhelm Bloch
This study evaluated the accuracy of the lactate minimum test, in comparison to a graded-exercise test and established threshold concepts (OBLA and mDmax) to determine running speed at maximal lactate steady state. Eighteen subjects performed a lactate minimum test, a graded-exercise test (2.4 m·s-1 start,+0.4 m·s-1 every 5 min) and 2 or more constant-speed tests of 30 min to determine running speed at maximal lactate steady state. The lactate minimum test consisted of an initial lactate priming segment, followed by a short recovery phase...
May 18, 2018: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/29775665/use-of-computational-fluid-dynamics-for-improving-freeze-dryers-design-and-process-understanding-part-1-modelling-the-lyophilisation-chamber
#13
Antonello A Barresi, Valeria Rasetto, Daniele L Marchisio
This manuscript shows how computational models, mainly based on Computational Fluid Dynamics (CFD), can be used to simulate different parts of an industrial freeze-drying equipment and to properly design them; in particular, the freeze-dryer chamber and the duct connecting the chamber with the condenser, with the valves and vanes eventually present are analysed in this work. In Part 1, it will be shown how CFD can be employed to improve specific designs, to perform geometry optimization, to evaluate different design choices and how it is useful to evaluate the effect on product drying and batch variance...
May 15, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29775546/leakage-rate-after-laparoscopic-ileocolic-intracorporeal-anastomosis
#14
Jesper Nors, Thorbjørn Sommer, Pål Wara
BACKGROUND AND AIMS: The prevailing technique in laparoscopic resection of the right colon has been laparoscopic-assisted procedure with externalization of the bowel for extracorporeal creation of the ileocolic anastomosis. The total laparoscopic technique performing all steps intracorporeally, however, has gained increasing interest. The purpose of this study was to describe our experience with creation of an ileocolic intracorporeal anastomosis (IIA) and to determine anastomotic leakage (AL) rate and short-term outcome of performing IIA...
May 18, 2018: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/29774776/memory-deficit-in-an-object-location-task-after-mild-tbi-is-associated-with-impaired-early-object-exploration-and-both-are-restored-by-branched-chain-amino-acid-dietary-therapy
#15
Rosalia Paterno, Hannah Metheny, Akiva S Cohen
The relation between traumatic brain injury (TBI) and memory dysfunction is well established, yet imprecise. Here, we investigate whether mild TBI causes a specific deficit in spatial episodic memory. Fifty-eight (29 TBI, 29 Sham) mice were run in a spatial recognition task. To determine which phase of memory might be affected in our task, we assessed rodent performance at three different delay times (3 min, 1 hour, and 24 hours). We found that sham and TBI mice performed equally well at 3 minutes, but TBI mice had significantly impaired spatial recognition memory after a delay time of one hour...
May 18, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29774032/protein-o-glcnac-modification-increases-in-white-blood-cells-after-a-single-bout-of-physical-exercise
#16
Tamás Nagy, Emese Kátai, Viktória Fisi, Tamás Tibor Takács, Antal Stréda, István Wittmann, Attila Miseta
Background: Protein O-linked N -acetylglucosamine (O-GlcNAc) is a dynamic posttranslational modification influencing the function of many intracellular proteins. Recently it was revealed that O-GlcNAc regulation is modified under various stress states, including ischemia and oxidative stress. Aside from a few contradictory studies based on animal models, the effect of exercise on O-GlcNAc is unexplored. Purpose: To evaluate O-GlcNAc levels in white blood cells (WBC) of human volunteers following physical exercise...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29773763/initial-experience-with-endoscopic-sleeve-gastroplasty-in-poland
#17
Aleksander Sowier, Przemysław Pyda, Anna-Maria Borucka, Sebastian Sowier, Jacek Białecki, Joanna Kapturzak, Stanisław Wójtowicz
INTRODUCTION: Obesity is becoming one of the major public health problems. Bariatric procedures are considered the most effective methods of treating this condition but they are costly and entail a high risk of complications. Thus, there is a need to look for better bariatric treatment solutions. One of the newest, highly promising bariatric methods is endoscopic sleeve gastroplasty (ESG), which is comparably effective to other bariatric procedures in terms of weight loss but at the same time it is much less invasive...
April 30, 2018: Polski Przeglad Chirurgiczny
https://www.readbyqxmd.com/read/29772904/why-cvd-grown-mos2-samples-outperform-pvd-samples-time-domain-ab-initio-analysis
#18
Linqiu Li, Run Long, Oleg V Prezhdo
Two-dimensional transition metal dichalcogenides (TMDs) have drawn strong attention due to their unique properties and diverse applications. However, TMD performance depends strongly on material quality and defect morphology. Experiments show that samples grown by chemical vapor deposition (CVD) outperform those obtained by physical vapor deposition (PVD). Experiments also show that CVD samples exhibit vacancy defects, while antisite defects are frequently observed in PVD samples. Our time-domain ab initio study demonstrates that both antisites and vacancies accelerate trapping and nonradiative recombination of charge carriers, but antisites are much more detrimental than vacancies...
May 18, 2018: Nano Letters
https://www.readbyqxmd.com/read/29771870/hyperfibrinolysis-is-an-important-cause-of-early-hemorrhage-in-patients-with-acute-promyelocytic-leukemia
#19
Yu-Hua Song, Chun Qiao, Li-Chan Xiao, Run Zhang, Hua - Lu
BACKGROUND The objective of the current study was to guide the early clinical treatment strategies by assessing the recovery of abnormal coagulation in acute promyelocytic leukemia (APL) patients during induction therapy. MATERIAL AND METHODS Retrospective analysis was performed in 112 newly-diagnosed patients with APL during induction treatment. RESULTS The early death (ED) rate in our study was 5.36% and the main cause was fetal hemorrhage. The presence of bleeding symptoms was significantly correlated with low platelet and fibrinogen levels...
May 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29770458/surfactant-effects-on-wettability-of-penicillium-frequentans-formulations-to-improve-brown-rot-biocontrol
#20
Belén Guijarro, Inmaculada Larena, Paloma Melgarejo, Antonieta De Cal
BACKGROUND: Penicillium frequentans could be approach to integrated pest management of brown rot, caused by Monilinia spp. Competition is primary mode of biocontrol activity of P. frequentans, and therefore, it must cover the entire fruit where the pathogen is acting. Our objective was to optimize P. frequentans efficacy, by maximizing fruit surface coverage and retention with the antagonist formulation by surfactant incorporation. RESULTS: Sixteen surfactants were assessed at 3-5 different concentrations and performance of disease-management of brown rot...
May 16, 2018: Journal of the Science of Food and Agriculture
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