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https://www.readbyqxmd.com/read/28735105/inherent-inferior-quality-of-follicular-fluid-in-repeat-breeder-heifers-as-evidenced-by-low-rates-of-in%C3%A2-vitro-production-of-bovine-embryos
#1
M Kafi, M Azari, O Chashnigir, S Gharibzadeh, Z Aghabozorgi, A Asaadi, M R Divar
The aims of the present study were to determine the effect of follicular fluid obtained from the ovulatory follicle of repeat breeder heifers on in vitro oocyte maturation (Experiment 1), fertilization (Experiment 2) and production of bovine embryos (Experiment 3). Holstein virgin heifers (VH, n = 5) with normal fertility or repeat breeder syndrome (RBH, n = 5) were used in the present study. Follicular fluid of VH and RBH was aspirated from ovulatory follicles and used as maturation medium. Bovine oocytes were aspirated from follicles of slaughterhouse ovaries and randomly allocated in three groups; in Group 1, oocytes cultured in TCM-199 supplemented with 10% heat-treated fetal calf serum and hormones (5 IU/mL hCG plus 0...
July 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/28733293/androgen-responsive-non-coding-small-rnas-extend-the-potential-use-of-hcg-stimulation-as-a-bioassay-of-androgen-sufficiency
#2
Martina Rodie, Manikhandan Av Mudaliar, Pawel Herzyk, M McMillan, Massoud Boroujerdi, Sandra Chudleigh, Edward S Tobias, S Faisal Ahmed
BACKGROUND: It is unclear whether a short-term change in circulating androgens is associated with changes in the transcriptome of the peripheral blood mononuclear cells (PBMC). AIMS & METHODS: To explore the effect of hCG-stimulation on the PBMC-transcriptome, 12 boys with a median age (range) of 0.7yrs (0.3, 11.2) who received intramuscular hCG 1500u on 3 consecutive days as part of their investigations underwent transcriptomic array analysis on RNA extracted from peripheral blood mononuclear cells before and after hCG stimulation...
July 21, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28724324/strong-impact-of-ethnicity-on-effectiveness-of-the-first-trimester-maternal-serum-screen-of-fetal-down-syndrome
#3
Thitikarn Lerthiranwong, Chanane Wanapirak, Supatra Sirichotiyakul, Fuanglada Tongprasert, Kasemsri Srisupundit, Suchaya Luewan, Theera Tongsong
OBJECTIVES: To compare the effectiveness of first trimester maternal serum screening (MSS) for fetal Down syndrome among Thai women between the method using Caucasian reference ranges with racial factor correction (CRR-RC) and that using Thai reference ranges (TRR). METHODS: A prospective database of MSS was accessed. The levels of PAPP-A and beta-hCG were calculated to determine their MoMs (multiple of medians) by two methods: 1) CRR-RC for Asian women and 2) TRR...
July 20, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28723610/pediatric-reference-intervals-for-29-ortho-vitros-5600-immunoassays-using-the-caliper-cohort-of-healthy-children-and-adolescents
#4
Victoria Higgins, Angela W S Fung, Man Khun Chan, Joseph Macri, Khosrow Adeli
BACKGROUND: Accurate reference intervals (RIs) based on a healthy pediatric population are essential for pediatric test result interpretation. The CALIPER project has recruited a large healthy cohort and completed a series of a priori studies to address gaps in pediatric RIs. As immunoassays from different manufacturers for endocrine and special chemistry markers are not standardized and show marked intermethod differences, direct RI studies are needed for each major analytical platform...
July 19, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28721181/adding-sildenafil-vaginal-gel-to-clomiphene-citrate-in-infertile-women-with-prior-clomiphene-citrate-failure-due-to-thin-endometrium-a-prospective-self-controlled-clinical-trial
#5
A N Fetih, D M Habib, I I Abdelaal, M Hussein, G N Fetih, E R Othman
We aimed to investigate the effect of adding sildenafil vaginal gel to clomiphene citrate (CC) in infertile women with prior CC failure. METHODS: This is a self- controlled clinical trial. Women with CC failure (in prior 5 cycles) and thin endometrium were recruited (N = 42). In their 6th (CC only) cycle, women continued on CC 100 mg/ day for 5 days, and had measurement of endometrial thickness and Doppler assessment of uterine arteries on day of HCG administration. In the 7th cycle, women (N = 36) were given usual dose of CC supplemented with sildenafil vaginal gel (5 gm, containing 50 mg sildenafil) twice daily from cycle day 8 to day of HCG administration...
March 2017: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/28721061/clinical-and-ultrasound-parameters-in-prediction-of-excessive-hemorrhage-during-management-of-cesarean-scar-pregnancy
#6
Ting Gui, Ping Peng, Xinyan Liu, Li Jin, Weilin Chen
PRECIS: During the management of cesarean scar pregnancy, gestational mass size >6 cm, uterine scar thickness <0.2 cm, peak systolic velocity >70 cm/s, and resistance index <0.35 are independent risk factors for excessive hemorrhage. OBJECTIVE: The objective of this study was to investigate risk factors associated with excessive hemorrhage during the management of cesarean scar pregnancy (CSP). PATIENTS AND METHODS: A retrospective case-control study was conducted, including 40 patients who experienced excessive bleeding and 80 controls without severe hemorrhage...
2017: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/28718082/low-first-trimester-papp-a-in-ivf-fresh-and-frozen-thawed-pregnancies-likely-due-to-a-biological-cause
#7
Lauren P Hunt, A M McInerney-Leo, S Sinnott, B Sutton, R Cincotta, G Duncombe, J Chua, M Peterson
PURPOSE: The purpose of this study is to confirm a difference in the first-trimester screen maternal biochemistry and false-positive rates (FPR) between pregnancies conceived spontaneously and those conceived via assisted reproductive technologies (ART). METHODS: Retrospective analysis of the complete population of women (17,889 pregnancies) who had undergone first-trimester screening between January 2004 and September 2009 at three private ultrasound clinics in Queensland, Australia was used in the study...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28715966/protective-effects-of-human-chorionic-gonadotropin-against-breast-cancer-how-can-we-use-this-information-to-prevent-treat-the-disease
#8
C V Rao
Breast cancers (BCs) are the most common malignancies among women worldwide. Giving birth to a first child before 24 years of age decreases the BC risk by about half, when women reach menopausal years. The scientific evidence suggests that the actions of human chorionic gonadotropin (hCG) are responsible for this decrease. Human BC cells and tissues contain hCG/luteinizing hormone receptors. The activation of the receptors results in an increase in cell differentiation and apoptosis. Conversely, it decreases the cell proliferation, invasion, and survival...
August 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28715965/dual-triggering-with-gonadotropin-releasing-hormone-agonist-and-standard-dose-human-chorionic-gonadotropin-in-patients-with-a-high-immature-oocyte-rate
#9
Alberta Maria Fabris, M Cruz, V Legidos, C Iglesias, M Muñoz, J A García-Velasco
In in vitro fertilization (IVF) cycles, some patients show a high rate of immature oocytes retrieved after controlled ovarian stimulation. In vitro oocyte maturation is an experimental technique, with poorer results than conventional IVF. For this reason, improving in vivo maturation could meliorate the reproductive outcome of these patients. We performed a retrospective, not interventional, study analyzing the difference in the number and percentage of mature oocytes retrieved in patients with more than 50% immature oocytes in a previous IVF cycle triggered with human chorionic gonadotropin (hCG) compared to the number and rate of mature oocytes retrieved in subsequent cycles, triggered with both gonadotropin-releasing hormone agonist (GnRH-a) and hCG...
August 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28715660/elevated-basal-progesterone-levels-are-associated-with-increased-preovulatory-progesterone-rise-but-not-with-higher-pregnancy-rates-in-icsi-cycles-with-gnrh-antagonists
#10
Mehmet Firat Mutlu, Mehmet Erdem, Ilknur Mutlu, Berk Bulut, Ahmet Erdem
OBJECTIVE: To ascertain the association between basal progesterone (P) levels and the occurrence of preovulatory progesterone rise (PPR) and clinical pregnancy rates (CPRs) in ICSI cycles with GnRH antagonists. STUDY DESIGN: Serum P levels of 464 patients were measured on day 2 and day of hCG of cycles. Cycles with basal P levels>1.6ng/mL were cancelled. All embryos were cryopreserved in cycles with P levels≥2ng/mL on the day of hCG. The primary outcome measures were the incidence of PPR (P>1...
June 29, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28715087/association-between-endometrial-subendometrial-vasculature-and-embryo-transfer-outcome-a-meta-analysis-and-subgroup-analysis
#11
Jianing Wang, Fei Xia, Ying Zhou, Xuedong Wei, Yanyan Zhuang, Yingxue Huang
OBJECTIVES: To examine the association between endometrial/subendometrial vasculature and in vitro fertilization-embryo transfer (IVF-ET) and frozen embryo transfer (FET) outcomes. METHODS: A meta-analysis of studies using endometrial/subendometrial 3-dimensional ultrasound and power Doppler angiography was performed to examine the vascularization index (VI), flow index (FI), and vascularization-flow index (VFI) in pregnant and nonpregnant women. Ten articles were analyzed, including 895 pregnant women and 882 nonpregnant women...
July 17, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28714317/prospective-observational-study-to-determine-the-accuracy-of-first-trimester-serum-biomarkers-and-uterine-artery-dopplers-in-combination-with-maternal-characteristics-and-arteriography-for-the-prediction-of-women-at-risk-of-preeclampsia-and-other-adverse-pregnancy
#12
Rebecca Allen, Joseph Aquilina
OBJECTIVES: To assess the efficacy of biomarkers, arteriography and uterine artery Dopplers for predicting hypertensive disease of pregnancy, small for gestational age and stillbirth. METHODS: This was a prospective first trimester study. Ultrasound was used to assess uterine artery Doppler. Maternal arteriography performed and serum taken for measurement of placental growth factor (PlGF), alpha fetoprotein (AFP), pregnancy associated plasma protein (PAPP-A) and beta human chorionic gonadotrophin (β-hCG) levels...
July 16, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28713970/human-chorionic-gonadotropin%C3%A2-%C3%AE-regulates-epithelial-mesenchymal-transition-and-metastasis-in-human-ovarian-cancer
#13
Na Liu, Shu-Min Peng, Guang-Xi Zhan, Jing Yu, Wei-Min Wu, Hao Gao, Xiao-Feng Li, Xiao-Qing Guo
Human chorionic gonadotropin β (β-hCG) is a well-known and accurate marker for the diagnosis and monitoring of pregnancy, trophoblastic tumors and ovarian germ cell tumors. Recently, β-hCG has been found to be closely related to poor prognosis and metastasis in various other malignant tumors, while its role and mechanism in ovarian cancer is still unclear. In the present study, lentiviral‑mediated transfection and small interfering RNA (siRNA) were used to alter β-hCG expression in the ovarian cancer cell lines ES-2 and SKOV3, respectively...
July 14, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28712790/prognostic-factors-for-tumor-recurrence-in-patients-with-clinical-stage-i-seminoma-undergoing-surveillance-a-systematic-review
#14
REVIEW
Friedemann Zengerling, Frank Kunath, Katrin Jensen, Christian Ruf, Stefanie Schmidt, Annabel Spek
OBJECTIVE: To systematically evaluate evidence on prognostic factors for tumor recurrence in patients with clinical stage I seminoma undergoing surveillance. METHODS: Systematic literature search conducted of Medline, Web of Science, Cochrane Library, and the conference proceedings of the ASCO, AUA, and EAU meetings (last search: October 2016), according to our prospectively registered protocol (PROSPERO registration number CRD42014009434). Identified records were reviewed according to the Cochrane Method Group of Prognosis Reviews recommendations and the PRISMA reporting guideline...
July 13, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28710674/what-is-the-best-predictor-of-severe-ovarian-hyperstimulation-syndrome-in-ivf-a-cohort-study
#15
Theoni B Tarlatzi, Christos A Venetis, Fabienne Devreker, Yvon Englert, Anne Delbaere
PURPOSE: The purpose of the present study is to study what is the best predictor of severe ovarian hyperstimulation syndrome (OHSS) in IVF. METHODS: This is a retrospective analysis of all consecutive IVF/intracytoplasmic injection cycles performed during a 5-year period (2009-2014) in a single university fertility centre. All fresh IVF cycles where ovarian stimulation was performed with gonadotrophins and GnRH agonists or antagonists and triggering of final oocyte maturation was induced with the administration of urinary or recombinant hCG were analyzed (2982 patients undergoing 5493 cycles)...
July 14, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28708875/predictive-value-of-serum-progesterone-level-on-%C3%AE-hcg-check-day-in-women-with-previous-repeated-miscarriages-after-in-vitro-fertilization
#16
Yong Jin Kim, Jung Ho Shin, Jun Yong Hur, Hoon Kim, Seung-Yup Ku, Chang Suk Suh
OBJECTIVE: To evaluate the predictive value of the progesterone level at the beta-human chorionic gonadotropin (β-hCG) check day for ongoing pregnancy maintenance in in vitro fertilization (IVF) cycles in women with previous unexplained repeated miscarriages. MATERIALS AND METHODS: One hundred and forty-eight women, with visible gestational sac after IVF, were recruited in this observational study. All subjects had unexplained recurrent miscarriages in more than two previous IVF cycles...
2017: PloS One
https://www.readbyqxmd.com/read/28708540/influence-of-two-ovulation-inducing-agents-on-the-pituitary-response-and-follicle-blood-flow-in-mares
#17
Y L Boakari, J C Ferreira, H S Canesin, D L Thompson, F S Lima, J C F Pantoja, C Meira
The objective of the current study was to evaluate the effects of deslorelin and hCG, two ovulation-inducing therapies, on LH surge and follicle vascularity in mares. Thirty mares were either treated with 1.5 mg IM of deslorelin, 2,500 IU IV of hCG or 2 mL IM of NaCl 0.9% (GnRH, hCG and Saline groups, respectively). Power-flow Doppler examination and blood collection were performed every hour during the first 12 hours after treatment (H0) and every six hours between hours 12 (H12) and 30 (H30) after treatment...
June 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28706366/gnrh-antagonist-cetrorelix-administration-before-hcg-for-protection-of-ovarian-hyperstimulation-syndrome
#18
Sherif A Hebisha, Banan A Aboelazm, H N Sallam
OBJECTIVE: Studying the effect of GnRH antagonist administration on the day of hCG to cases of IVF/ICSI with estradiol level above 5000 ng/dl for protection of ovarian hyperstimulation syndrome. DESIGN: Prospective study. MATERIALS AND METHODS: Sixty patients undergoing controlled hyperstimulation COH, for IVF/ICSI using long agonist and E2 level on the day of hCG, are above 5000 ng/dl, 52 patients received single dose of cetrorelix 0.25 mg on the day of hCG, and 8 patients received two doses of 0...
August 2017: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/28704926/a-label-free-and-ultrasensitive-immunosensor-for-detection-of-human-chorionic-gonadotrophin-based-on-graphene-fets
#19
Kamrul Islam, Ahmed Suhail, Genhua Pan
We report on a label-free immunosensor based on graphene field effect transistors (G-FETs) for the ultrasensitive detection of Human Chorionic Gonadotrophin (hCG), as an indicator of pregnancy and related disorders, such as actopic pregnancy, choriocarcinoma and orchic teratoma. Pyrene based bioactive ester was non-covalently anchored onto the graphene channel in order to retain the sp² lattice. The G-FET transfer characteristics showed repeatable and reliable responses in all surface modifying steps using a direct current (DC) readout system...
July 12, 2017: Biosensors
https://www.readbyqxmd.com/read/28702979/use-of-platelet-rich-plasma-in-human-infertility
#20
G V L Colombo, V Fanton, D Sosa, E Criado Scholz, J Lotti, S E Aragona, T Lotti
Successful embryo implantation requires good quality embryo but also needs a receptive endometrium site. In our clinical practice, we daily verify that an adequate endometrial growth is reached for successful implantation. To understand whether platelet rich plasma (PRP) can improve endometrium thickness and performance, PRP treatment was carried out after at least three of the classic medical protocols currently in use had been unsuccessfully adopted. Eight patients with more than 3 cryo-transfers cancelled because of failure of endometrial growth, defined as endometrium less than 6 mm, with negative hysteroscopic screening for endometrial pathology, and with negative bacteriologic screening, before present and all previous treatment, were selected to undergo PRP treatment...
July 13, 2017: Journal of Biological Regulators and Homeostatic Agents
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