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https://www.readbyqxmd.com/read/29164502/superovulation-alters-dna-methyltransferase-protein-expression-in-mouse-oocytes-and-early-embryos
#1
Fatma Uysal, Saffet Ozturk, Gokhan Akkoyunlu
PURPOSE: DNA methylation is an epigenetic mechanism that plays critical roles during mammalian oocyte and preimplantation embryo development. It is achieved by adding a methyl group to the fifth carbon atom of cytosine residues within cytosine-phosphate-guanine (CpG) and non-CpG dinucleotide sites using DNA methyltransferase (DNMT) enzymes for de novo and maintenance methylation processes. DNMT1, DNMT3A, and DNMT3B play important roles in establishing methylation of developmentally related genes in oocytes and early embryos...
November 22, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29164084/pretreatment-with-human-chorionic-gonadotropin-protects-the-neonatal-brain-against-the-effects-of-hypoxic-ischemic-injury
#2
Tammy Z Movsas, Rebecca L Weiner, M Banks Greenberg, David M Holtzman, Rafael Galindo
Introduction: Though the human fetus is exposed to placentally derived human chorionic gonadotropin (hCG) throughout gestation, the role of hCG on the fetal brain is unknown. Review of the available literature appears to indicate that groups of women with higher mean levels of hCG during pregnancy tend to have offspring with lower cerebral palsy (CP) risk. Given that newborn cerebral injury often precedes the development of CP, we aimed to determine whether hCG may protect against the neurodegenerative effects of neonatal brain injury...
2017: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/29162950/intrauterine-insemination-fundamentals-revisited
#3
EDITORIAL
Gautam N Allahbadia
Intrauterine insemination (IUI) is an assisted conception technique that involves the deposition of a processed semen sample in the upper uterine cavity, overcoming natural barriers to sperm ascent in the female reproductive tract. It is a cost-effective, noninvasive first-line therapy for selected patients with functionally normal tubes, and infertility due to a cervical factor, anovulation, moderate male factor, unexplained factors, immunological factor, and ejaculatory disorders with clinical pregnancy rates per cycle ranging from 10 to 20%...
December 2017: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/29161658/reproductive-performance-of-lactating-dairy-cows-after-inducing-ovulation-using-hcg-in-a-five-day-progesterone-based-fixed-time-ai-protocol
#4
I Garcia-Ispierto, F De Rensis, X Casas, F Caballero, R Mur-Novales, F López-Gatius
This study compares the effects of inducing ovulation using human chorionic gonadotropin (hCG) or gonadotropin releasing hormone (GnRH) at the end of a 5-day progesterone(P4)-based protocol for fixed-time artificial insemination (FTAI) in lactating dairy cows on ovarian dynamics and fertility. A P4 intravaginal device (PRID) was fitted for five days and GnRH administered upon PRID insertion and a double dose (24 h apart) of prostaglandin F2α upon PRID removal. Cows received either GnRH (GnRH group; n = 98), 1000 IU hCG (hCG-1 group; n = 97), or 3000 IU hCG (hCG-2 group; n = 104) 36 h after PRID removal and were inseminated 50-56 h after PRID removal...
November 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/29155477/oxidized-low-density-lipoprotein-suppresses-mouse-granulosa-cell-differentiation-through-disruption-of-the-hypoxia-inducible-factor-1-pathway
#5
Xiaoliang Chen, Yanhong Liu, Yanhong Shan, Xingyi Jin, Qingyang Shi, Chunshu Jia
Obesity predisposes women to reproductive disorders. One symptom of obesity in women is higher levels of oxidized Low-density lipoprotein (oxLDL) in serum and preovulatory follicles. The present study was designed to test the hypothesis that oxLDL might impair follicle differentiation and luteinization. Given that Hypoxia-inducible factor 1 (HIF1) plays crucial roles in supporting follicle differentiation and luteinization in mammals, we focused on oxLDL-mediated events that may affect the HIF1 pathway. We report that exposure to oxLDL diminished the expression of HIF1α and its target genes and suppressed the differentiation of mouse luteinized granulosa cells following induction by human Chorionic gonadotophin (hCG) under hypoxic conditions (1% oxygen)...
November 20, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29154410/predicting-success-of-methotrexate-treatment-by-pretreatment-hcg-level-and-24-hour-hcg-increment
#6
Gabriel Levin, Narjes A Saleh, Rani Haj-Yahya, Liat S Matan, Avi Benshushan
OBJECTIVE: To evaluate β-human chorionic gonadotropin (β-HCG) level and its 24-hour increment as predictors of successful methotrexate treatment for ectopic pregnancy. METHODS: Data were retrospectively reviewed from women with ectopic pregnancy who were treated by single-dose methotrexate (50 mg/m(2) ) at a university hospital in Jerusalem, Israel, between January 1, 2000, and June 30, 2015. Serum β-HCG before treatment and its percentage increment in the 24 hours before treatment were compared between treatment success and failure groups...
November 20, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/29152085/the-effect-of-human-chorionic-gonadotrophin-contained-in-human-menopausal-gonadotropin-on-the-clinical-outcomes-during-progestin-primed-ovarian-stimulation
#7
Xiuxian Zhu, Jing Ye, Yonglun Fu, Ai Ai, Renfei Cai, Yun Wang, Qingging Hong, Tian Hui, Qifeng Lyu, Qiuju Chen, Yanping Kuang
Progestin-primed ovarian stimulation (PPOS) protocol has recently been demonstrated to be an novel regimen for preventing premature LH surges during controlled ovarian hyperstimulation (COH) in combination with frozen-thawed embryo transfer (FET). Our prospective controlled study was to explore the effect of human chorionic gonadotropin (hCG) contained in human menopausal gonadotropin (hMG) on the clinical outcomes in normalovulatory women undergoing COH with PPOS. A total of 180 patients were allocated into three groups according to the gonadotropin (Gn) used: group A (human menopausal gonadotropin, hMG-A), group B (hMG-B) or group C (follicle stimulating hormone, FSH)...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29147846/hcg-administration-after-endogenous-lh-rise-negatively-influences-pregnancy-rate-in-modified-natural-cycle-for-frozen-thawed-euploid-blastocyst-transfer-a-pilot-study
#8
Katarzyna Litwicka, Cecilia Mencacci, Cristiana Arrivi, Maria Teresa Varricchio, Alina Caragia, Maria Giulia Minasi, Ermanno Greco
PURPOSE: The aim of the present study was to evaluate whether in a modified natural cycle (modified-NC) for a frozen-thawed single euploid blastocyst transfer, a critical LH value, above which human chorionic gonadotropin (hCG) administration should be avoided, may be defined. METHODS: One hundred and sixty-seven patients underwent modified natural cycle in order to transfer a single frozen-thawed euploid blastocyst. All embryos were obtained by intracytoplasmic sperm injection and were biopsied at the blastocyst stage and analyzed by means of array comparative genomic hybridization (aCGH)...
November 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29144987/benzo-a-pyren-7-8-dihydrodiol-9-10-epoxide-induces-human-trophoblast-swan-71-cell-dysfunctions-due-to-cell-apoptosis-through-disorder-of-mitochondrial-fission-fusion
#9
Weiping Wang, Rong Wang, Qiao Zhang, Gil Mor, Huidong Zhang
Benzo(a)pyren-7,8-dihydrodiol-9,10-epoxide (BPDE) is an endocrine disrupter and ultimate carcinogenic product of benzo(a)pyrene (BaP). Numerous studies have shown that BPDE causes trophoblast-related diseases, such as preeclampsia, growth restriction or miscarriages. However, the underlying mechanism, especially the mitochondria-related BPDE-induced trophoblast dysfunction remains unknown. In this study, we examined mitochondrial functions in BPDE-induced human trophoblast cell line Swan 71. BPDE decreased cell ability, attenuated cell invasion and HCG secretion, induced cell apoptosis, decreased mitochondrial membrane potential, increased reactive oxygen species (ROS) and MDA, and decreased SOD activity in a dose-dependent manner...
November 13, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29142450/autologous-intrauterine-platelet-rich-plasma-instillation-for-suboptimal-endometrium-in-frozen-embryo-transfer-cycles-a-pilot-study
#10
Sunita R Tandulwadkar, Manasi V Naralkar, Akash D Surana, M Selvakarthick, Avinash H Kharat
Introduction: This pilot study was to evaluate the effectiveness of intrauterine infusion of autologous platelet-rich plasma (PRP) in infertile women undergoing frozen embryo transfer cycles with suboptimal endometrium. Material and Methods: Intrauterine instillation of autologous PRP was done in 68 women between 22 and 40 years, over 8 months, with suboptimal endometrial growth, and patients with repeated cycle cancellations, in addition to Estradiol valerate. Frozen embryo transfer was performed when the endometrium reached an optimal pattern in terms of thickness, appearance, and vascularity...
July 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29142445/a-study-of-controlled-ovarian-stimulation-with-clomiphene-citrate-or-letrozole-in-combination-with-gonadotropins-and-iui-in-unexplained-infertility
#11
Madhusmita Hembram, Ratna Biswas, Anju Jain
Aim: To compare the effect of clomiphene citrate (CC) + human menopausal gonadotropin (hMG) with letrozole + hMG on size, number of follicles, endometrial thickness, serum levels of oestradiol and progesterone and pregnancy rate. Settings and Design: Non-randomised interventional study. Patients and Methods: A total number of 60 patients in the age group of 20-35 years with unexplained infertility were divided into two groups, 30 in each. Group A received CC + hMG and group B received letrozole + hMG...
July 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29139356/-persistent-trophoblast-tissue-after-salpingectomy
#12
EDITORIAL
Louise Stig Hornstrup, Bjarke Lunde Sørensen, Vicky Jenny-Rebecka Wetterstrand
We present a case of persistent trophoblast tissue (PT) five weeks after salpingectomy for tubal pregnancy. The fallopian tube-sparing method (salpingotomy) has a greater risk of PT than removal of the whole fallopian tube (salpingectomy) has. A 32-year-old woman was treated with salpingectomy on suspicion of a bleeding ectopic pregnancy and was readmitted due to PT. There is no evidence for measuring the human chorionic gonadotropin (hCG) level as routine follow-up after salpingectomy, but it is important to be aware of the risk of PT and if in doubt measure the levels of hCG...
November 13, 2017: Ugeskrift for Laeger
https://www.readbyqxmd.com/read/29131089/human-chorionic-gonadotropin-test-old-uncertainties-new-perspectives-and-value-in-46-xy-disorders-of-sex-development
#13
Silvano Bertelloni, Gianni Russo, Giampiero I Baroncelli
The human chorionic gonadotropin (hCG) test represents a key step in assessing Leydig cell function in prepubertal males, but differences in terms of hCG doses, number of injections, and sequence of blood drawing are published in the literature, showing poor standardization. The few available data in healthy boys are summarized here. A recombinant hCG (rhCG) formulation might permit overcoming some controversies as well as avoid the potential biological risk related to the injection of extractive hormones. Studies in humans are scarce, but they indicate that 250 µg rhCG in a single dose may represent a useful scheme for the dynamic evaluation of Leydig cell function in children as well as in adults...
November 2, 2017: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/29129877/evaluation-of-the-chemiluminescent-enzyme-immunoassay-system-for-the-measurement-of-testosterone-in-the-serum-and-whole-blood-of-stallions
#14
Yuko Toishi, Nobuo Tsunoda, Shun-Ichi Nagata, Rikio Kirisawa, Kentaro Nagaoka, Gen Watanabe, Yojiro Yanagawa, Seiji Katagiri, Kazuyoshi Taya
Testosterone (T) concentration is a useful indicator of reproductive function in male animals. However, T concentration is not usually measured in veterinary clinics, partly due to the unavailability of reliable and rapid assays for animal samples. In this study, a rapid chemiluminescent enzyme immunoassay system (CLEIA system) that was developed for the measurement of T concentration in humans use was validated for stallion blood samples. First, serum T concentrations were measured using the CLEIA system and compared with those measured by a fluoroimmunoassay that has been validated for use in stallions...
November 11, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29129601/on-chip-signal-amplification-of-magnetic-bead-based-immunoassay-by-aviating-magnetic-bead-chains
#15
Uddin M Jalal, Gyeong Jun Jin, Kyu Shik Eom, Min Ho Kim, Joon S Shim
In this work, a Lab-on-a-Chip (LOC) platform is used to electromagnetically actuate magnetic bead chains for an enhanced immunoassay. Custom-made electromagnets generate a magnetic field to form, rotate, lift and lower the magnetic bead chains (MBCs). The cost-effective, disposable LOC platform was made with a polymer substrate and an on-chip electrochemical sensor patterned via the screen-printing process. The movement of the MBCs is controlled to improve the electrochemical signal up to 230% when detecting beta-type human chorionic gonadotropin (β-hCG)...
November 6, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29126090/the-vanishing-follicle-in-women-with-low-number-of-developing-follicles-during-assisted-reproduction
#16
Johnny S Younis, Shiran Yakovi, Ido Izhaki, Sami Haddad, Moshe Ben-Ami
OBJECTIVE: To investigate the occurrence of the "vanishing follicle" phenomenon in women with low number of developing follicles in assisted reproduction. STUDY DESIGN: Women with ≤ 6 follicles on the day of hCG administration with ≥ 14mm diameter were prospectively studied. Primary outcome measures were disappearance of ≥14mm and all-diameter follicles on the day of oocyte pick-up compared to the day of hCG administration. RESULTS: Among the 120 women recruited, 95 were found eligible and completed the study...
November 3, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29120657/-anabolic-steroid-induced-hypogonadism-in-men-overview-and-case-report
#17
Luboslav Stárka, Michaela Dušková, Lucie Kolátorová, Oldřich Lapčík
An important potential consequence of the anabolic steroid misuse is hypogonadotropic hypogonadism due to the inhibition of pituitary secretion of gonadotropins. By the symptoms as testicular atrophy, spermatogenic and fertility disturbances or dysfunction in sexual life, the anabolic steroids induced hypogonadism (ASIH) could be differentiated from organic hypogonadotropic hypogonadism only with difficulty unless the misuse is reported by the user. When diagnosed, the crucial step in the therapy is the stop of anabolic use...
2017: Vnitr̆ní Lékar̆ství
https://www.readbyqxmd.com/read/29120551/current-medical-strategies-in-the-prevention-of-ovarian-hyperstimulation-syndrome
#18
Miro Kasum, Slavko Orešković, Daniela Franulić, Ermin Čehić, Albert Lila, Goran Vujić, Franjo Grgić
The purpose of this review is to analyze current medical strategies in the prevention of ovarian hyperstimulation syndrome (OHSS) during ovarian stimulation for in vitro fertilization. Owing to contemporary preventive measures of OHSS, the incidence of moderate and severe forms of the syndrome varies between 0.18% and 1.40%. Although none of medical strategies is completely effective, there is high-quality evidence that replacing human chorionic gonadotropin (hCG) by gonadotropin-releasing hormone (GnRH) agonists after GnRH antagonists and moderate- quality evidence that GnRH antagonist protocols, dopamine agonists and mild protocols reduce the occurrence of OHSS...
March 2017: Acta Clinica Croatica
https://www.readbyqxmd.com/read/29119996/reversion-of-gold-nanoparticle-aggregates-for-the-detection-of-cu-2-and-its-application-in-immunoassays
#19
Chia-Chen Chang, Chung-Han Lee, Tzu-Heng Wu, Chie-Pein Chen, Chen-Yu Chen, Chii-Wann Lin
A high concentration of copper is a hazardous element to organisms and human health. Although various strategies have been reported for the sensitive detection of copper, a facile and rapid detection of aqueous copper has seldom been addressed to date. Here, we present an easy and accessible colorimetric method to detect Cu(2+) using the redispersion of cysteamine-modified gold nanoparticles (CA-AuNPs). Initially, CA caused the aggregation of AuNPs due to the electrostatic interaction and aggregated AuNPs can be regenerated in basic medium...
November 9, 2017: Analyst
https://www.readbyqxmd.com/read/29119546/a-systematic-review-of-ectopic-pregnancy-after-hysterectomy
#20
REVIEW
Emily X Shao, Kendra Hopper, Matthew McKnoulty, Alka Kothari
BACKGROUND: An ectopic pregnancy after hysterectomy is a rare but potentially life-threatening event. Women with this condition might not be appropriately investigated, resulting in delays in diagnosis and treatment. OBJECTIVES: To characterize cases of ectopic pregnancy occurring after hysterectomy. SEARCH STRATEGY: PubMed, Embase, Scopus, and Web of Science were searched using the terms "pregnancy, abdominal" or "pregnancy, tubal" or "pregnancy, ectopic" and "hysterectomy" or "post-hysterectomy" or "post hysterectomy...
November 9, 2017: International Journal of Gynaecology and Obstetrics
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