keyword
MENU ▼
Read by QxMD icon Read
search

h2ax and irradiation

keyword
https://www.readbyqxmd.com/read/28797171/novel-composite-drug-delivery-system-as-a-novel-radio-sensitizer-for-the-local-treatment-of-cervical-carcinoma
#1
Shan Xu, Yu Ying Tang, Yan Xin Yu, Qin Yun, Jing Pin Yang, Heng Zhang, Qiuxia Peng, Xiaoyang Sun, Ling Lin Yang, ShaoZhi Fu, Jing Bo Wu
In this study, we investigated in vivo radiosensitizing effects of a gel-based dual drug delivery system (DDS) (PECE/DDP + mPEG-PCL/PTX, or PDMP) in a cervical cancer model, and determined its possible mechanisms of action. A xenograft cervical cancer model was used to investigate the radio sensitization effect of PDMP. Mice underwent paclitaxel (PTX) + cisplatin (DDP), PECE, or PDMP treatment followed by single radiation doses ranging from 0 Gy to 20 Gy. Radio sensitization was analyzed by tumor regrowth delay (TGD)...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28794020/adenovirus-core-protein-vii-down-regulates-the-dna-damage-response-on-the-host-genome
#2
Daphne C Avgousti, Ashley N Della Fera, Clayton J Otter, Christin Herrmann, Neha J Pancholi, Matthew D Weitzman
Viral manipulation of cellular proteins allows viruses to suppress host defenses and generate infectious progeny. Due to the linear double-stranded DNA nature of the adenovirus genome, the cellular DNA damage response (DDR) is considered a barrier for successful infection. The adenovirus genome is packaged with protein VII, a viral-encoded histone-like core protein that is suggested to protect incoming viral genomes from detection by cellular DNA damage machinery. We showed that protein VII localizes to host chromatin during infection, leading us to hypothesize that protein VII may affect DNA damage responses on the cellular genome...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28766757/sesamol-ameliorates-radiation-induced-dna-damage-in-hematopoietic-system-of-whole-body-%C3%AE-irradiated-mice
#3
Arun Kumar, Sandeep Choudhary, Jawahar S Adhikari, Nabo K Chaudhury
Ionizing radiation exposure is harmful and at high doses can lead to acute hematopoietic radiation syndrome. Therefore, agents that can protect hematopoietic system are important for development of radioprotector. Sesamol is a potential molecule for development of radioprotector due to its strong free radical scavenging and antioxidant properties. In the present study, sesamol was evaluated for its role in DNA damage and repair in hematopoietic system of γ-irradiated CB57BL/6 mice and compared with amifostine...
August 2, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/28753066/a-single-exposure-to-low-or-high-let-radiation-induces-persistent-genomic-damage-in-mouse-epithelial-cells-in-vitro-and-in-lung-tissue
#4
Erica Werner, Ya Wang, Paul W Doetsch
Exposures to low- and high-linear energy transfer (LET) radiation induce clustered damage in DNA that is difficult to repair. These lesions are manifested as DNA-associated foci positive for DNA repair proteins and have been shown to persist in vitro and in vivo for days in several cell types and tissues in response to low-LET radiation. Although in some experimental conditions these residual foci have been linked with genomic instability and chromosomal aberrations, it remains poorly understood what type of damage they represent...
July 28, 2017: Radiation Research
https://www.readbyqxmd.com/read/28741984/multiple-ct-scans-extend-lifespan-by-delaying-cancer-progression-in-cancer-prone-mice
#5
Jennifer A Lemon, Nghi Phan, Douglas R Boreham
Computed tomography (CT) scans are a routine diagnostic imaging technique that utilize low-energy X rays with an average absorbed dose of approximately 10 mGy per clinical whole-body CT scan. The growing use of CT scans in the clinic has raised concern of increased carcinogenic risk in patients exposed to ionizing radiation from diagnostic procedures. The goal of this study was to better understand cancer risk associated with low-dose exposures from CT scans. Historically, low-dose exposure preceding a larger challenge dose increases tumor latency, but does little to impact tumor frequency in Trp53(+/-) mice...
July 25, 2017: Radiation Research
https://www.readbyqxmd.com/read/28735740/dna-damage-and-the-bystander-response-in-tumor-and-normal-cells-exposed-to-x-rays
#6
M Subhashree, R Venkateswarlu, K Karthik, V Shangamithra, P Venkatachalam
Monolayer and suspension cultures of tumor (BMG-1, CCRF-CEM), normal (AG1522, HADF, lymphocytes) and ATM-mutant (GM4405) human cells were exposed to X-rays at doses used in radiotherapy (high dose and high dose-rate) or radiological imaging (low dose and low dose-rate). Radiation-induced DNA damage, its persistence, and possible bystander effects were evaluated, based on DNA damage markers (γ-H2AX, p53(ser15)) and cell-cycle-specific cyclins (cyclin B1 and cyclin D1). Dose-dependent DNA damage and a dose-independent bystander response were seen after exposure to high dose and high dose-rate radiation...
September 2017: Mutation Research
https://www.readbyqxmd.com/read/28689138/enhanced-dna-double-strand-break-repair-of-microbeam-targeted-a549-lung-carcinoma-cells-by-adjacent-wi38-normal-lung-fibroblast-cells-via-bi-directional-signaling
#7
Alisa Kobayashi, Tengku Ahbrizal Farizal Tengku Ahmad, Narongchai Autsavapromporn, Masakazu Oikawa, Shino Homma-Takeda, Yoshiya Furusawa, Jun Wang, Teruaki Konishi
Understanding the mechanisms underlying the radiation-induced bystander effect (RIBE) and bi-directional signaling between irradiated carcinoma cells and their surrounding non-irradiated normal cells is relevant to cancer radiotherapy. The present study investigated propagation of RIBE signals between human lung carcinoma A549 cells and normal lung fibroblast WI38 cells in bystander cells, either directly or indirectly contacting irradiated A549 cells. We prepared A549-GFP/WI38 co-cultures and A549-GFP/A549 co-cultures, in which A549-GFP cells stably expressing H2BGFP were co-cultured with either A549 cells or WI38 cells, respectively...
June 23, 2017: Mutation Research
https://www.readbyqxmd.com/read/28688881/the-abdominal-skin-of-female-sprague-dawley-rats-is-more-sensitive-than-the-back-skin-to-drug-induced-phototoxicity
#8
Kazuhiro Kuga, Hironobu Yasuno, Yumi Sakai, Yumiko Harada, Fumi Shimizu, Yumiko Miyamoto, Yuki Takamatsu, Makoto Miyamoto, Keiichiro Sato
In vivo phototoxicity studies are important to predict drug-induced phototoxicity in humans; however, a standard methodology has not established. To determine differences in sensitivity to drug-induced phototoxicity among various skin sites, we evaluated phototoxic reactions in the back and abdominal skin of female Sprague-Dawley rats orally dosed with phototoxic drugs (pirfenidone, 8-methoxysoraren, doxycycline, and lomefloxacin) or a non-phototoxic drug (gatifloxacin) followed by solar-simulated light irradiation comprising 18J/cm(2) ultraviolet A...
July 5, 2017: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/28685056/pml-silencing-inhibits-cell-proliferation-and-induces-dna-damage-in-cultured-ovarian-cancer-cells
#9
Sheng-Bing Liu, Zhong-Fei Shen, Yan-Jun Guo, Li-Xian Cao, Ying Xu
The promyelocytic leukemia (PML) gene is a tumor suppressor gene. It was first identified in acute promyelocytic leukemia, in which it is fused to retinoic acid receptor α by the (15;17) chromosomal translocation. The function of the PML protein is frequently lost or aberrant in human solid tumors. In human ovarian carcinoma tissue, PML detected by immunohistochemistry was highly expressed. A PML-silencing vector, pSRG-shPml, was constructed and used to transfect human ovarian cancer cells. Cells were cultured and selected with puromycin for 10-15 days, and then the PML mRNA expression levels were detected by RT-qPCR and immunofluorescence...
July 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28681635/estimation-of-low-dose-radiation-responsive-proteins-in-the-absence-of-genomic-instability-in-normal-human-fibroblast-cells
#10
Ji-Hye Yim, Jung Mi Yun, Ji Young Kim, Seon Young Nam, Cha Soon Kim
PURPOSE: Low-dose radiation has various biological effects such as adaptive responses, low-dose hypersensitivity, as well as beneficial effects. However, little is known about the particular proteins involved in these effects. Here, we sought to identify low-dose radiation-responsive phosphoproteins in normal fibroblast cells. MATERIALS AND METHODS: We assessed genomic instability and proliferation of fibroblast cells after γ-irradiation by γ-H2AX foci and micronucleus formation analyses and BrdU incorporation assay, respectively...
July 6, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28673358/difference-in-the-relative-biological-effectiveness-and-dna-damage-repair-processes-in-response-to-proton-beam-therapy-according-to-the-positions-of-the-spread-out-bragg-peak
#11
Hidehiro Hojo, Takeshi Dohmae, Kenji Hotta, Ryosuke Kohno, Atsushi Motegi, Atsushi Yagishita, Hideki Makinoshima, Katsuya Tsuchihara, Tetsuo Akimoto
BACKGROUND: Cellular responses to proton beam irradiation are not yet clearly understood, especially differences in the relative biological effectiveness (RBE) of high-energy proton beams depending on the position on the Spread-Out Bragg Peak (SOBP). Towards this end, we investigated the differences in the biological effect of a high-energy proton beam on the target cells placed at different positions on the SOBP, using two human esophageal cancer cell lines with differing radiosensitivities...
July 3, 2017: Radiation Oncology
https://www.readbyqxmd.com/read/28631426/hsp90%C3%AE-regulates-atm-and-nbn-functions-in-sensing-and-repair-of-dna-double-strand-breaks
#12
EDITORIAL
Rosa Pennisi, Antonio Antoccia, Stefano Leone, Paolo Ascenzi, Alessandra di Masi
The molecular chaperone heat shock protein 90 (Hsp90α) regulates cell proteostasis and mitigates the harmful effects of endogenous and exogenous stressors on the proteome. Indeed, the inhibition of Hsp90α ATPase activity affects the cellular response to ionizing radiation (IR). Although the interplay between Hsp90α and several DNA damage response (DDR) proteins has been reported, its role in the DDR is still unclear. Here, we show that ataxia-telangiectasia-mutated kinase (ATM) and nibrin (NBN), but not 53BP1, RAD50, and MRE11, are Hsp90α clients as the Hsp90α inhibitor 17-(allylamino)-17-demethoxygeldanamycin (17-AAG) induces ATM and NBN polyubiquitination and proteosomal degradation in normal fibroblasts and lymphoblastoid cell lines...
August 2017: FEBS Journal
https://www.readbyqxmd.com/read/28624023/enhanced-radiation-damage-caused-by-iodinated-contrast-agents-during-ct-examination
#13
Ling Wang, Qiang Li, Xi-Ming Wang, Guang-Yu Hao, Jie-Bao, Su Hu, Chun-Hong Hu
OBJECTIVE: To access the effect of iodinate contrast agent (ICA) on DNA double-stand breaks (DSBs) in human peripheral blood lymphocytes during computed tomography (CT) examinations. MATERIALS AND METHODS: This present study was approved by the institutional ethics committee; written informed patient consent was obtained from 70 patients. A total of 48 patients underwent computed tomography urography (CTU), in which only one time CT scanning was examined after injecting ICA, and 22 patients received unenhanced whole abdominal CT, among them 10 patients were selected to get ICA injection immediately after irradiation...
July 2017: European Journal of Radiology
https://www.readbyqxmd.com/read/28599315/reevaluation-of-atr-signaling-in-primary-resting-chronic-lymphocytic-leukemia-cells-evidence-for-pro-survival-or-pro-apoptotic-function
#14
Maxime Beyaert, Eliza Starczewska, Ana Cristina González Pérez, Nicolas Vanlangendonck, Pascale Saussoy, Gaëlle Tilman, Anne De Leener, Marie-Christiane Vekemans, Eric Van Den Neste, Françoise Bontemps
ATM, primarily activated by DNA double-strand breaks, and ATR, activated by single-stranded DNA, are master regulators of the cellular response to DNA damage. In primary chronic lymphocytic leukemia (CLL) cells, ATR signaling is considered to be switched off due to ATR downregulation. Here, we hypothesized that ATR, though expressed at low protein level, could play a role in primary resting CLL cells after genotoxic stress. By investigating the response of CLL cells to UV-C irradiation, a prototypical activator of ATR, we could detect phosphorylation of ATR at Thr-1989, a marker for ATR activation, and also observed that selective ATR inhibitors markedly decreased UV-C-induced phosphorylation of ATR targets, including H2AX and p53...
May 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28587165/dynamic-in-vivo-profiling-of-dna-damage-and-repair-after-radiotherapy-using-canine-patients-as-a-model
#15
Nadine Schulz, Hassan Chaachouay, Katarzyna J Nytko, Mathias S Weyland, Malgorzata Roos, Rudolf M Füchslin, Franco Guscetti, Stephan Scheidegger, Carla Rohrer Bley
Time resolved data of DNA damage and repair after radiotherapy elucidates the relation between damage, repair, and cell survival. While well characterized in vitro, little is known about the time-course of DNA damage response in tumors sampled from individual patients. Kinetics of DNA damage after radiotherapy was assessed in eight dogs using repeated in vivo samples of tumor and co-irradiated normal tissue analyzed with comet assay and phosphorylated H2AX (γH2AX) immunohistochemistry. In vivo results were then compared (in silico) with a dynamic mathematical model for DNA damage formation and repair...
June 1, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28565765/pravastatin-reduces-radiation-induced-damage-in-normal-tissues
#16
Hiroshi Doi, Seiji Matsumoto, Soichi Odawara, Toshiyuki Shikata, Kazuhiro Kitajima, Masao Tanooka, Yasuhiro Takada, Tohru Tsujimura, Norihiko Kamikonya, Shozo Hirota
Pravastatin is an inhibitor of 3-hydroxy-3-methyl- glutaryl-coenzyme A reductase that has been reported to have therapeutic applications in a range of inflammatory conditions. The aim of the present study was to assess the radioprotective effects of pravastatin in an experimental animal model. Mice were divided into two groups: The control group received ionizing radiation with no prior medication, while the pravastatin group received pravastatin prior to ionizing radiation. Pravastatin was administered orally at 30 mg/kg body weight in drinking water at 24 and 4 h before irradiation...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28544661/early-passage-mesenchymal-stem-cells-display-decreased-radiosensitivity-and-increased-dna-repair-activity
#17
Po-Kuei Wu, Jir-You Wang, Cheng-Fong Chen, Kuang-Yu Chao, Ming-Chau Chang, Wei-Ming Chen, Shih-Chieh Hung
Cell therapies using human mesenchymal stem cells (MSCs) have received much attention in the past decade. In pursuit of the therapeutic potential of MSCs, cell expansion is required to generate a great number of cells with desired phenotype and functionality. Long-term expansion in vitro, however, can lead to altered functions. To explore the changes in DNA damage responses (DDR) in MSCs expanded, DDR pathways following irradiation were characterized in early- and late-passage bone marrow MSCs. Seventy-two hours after irradiation, the percentage of sub-G1 cells in early-passage MSCs did not change significantly...
June 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28503683/cellular-evaluation-of-diselenonicotinamide-dsna-as-a-radioprotector-against-cell-death-and-dna-damage
#18
M Raghuraman, Prachi Verma, Amit Kunwar, Prasad P Phadnis, V K Jain, K Indira Priyadarsini
Diselenonicotinamide (DSNA), a synthetic organoselenium compound, was evaluated for its radioprotective effect in cellular models. A clonogenic assay in Chinese Hamster Ovary (CHO) cells and an apoptosis assay in murine splenic lymphocytes indicated that pre-treatment with DSNA at a concentration of 25 μM significantly protected them from radiation-induced cell death. Upon irradiation (1-12 Gy), dose-response studies were carried out under similar treatment conditions, and its dose modification factor (DMF) was estimated to be 1...
June 1, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28494188/depletion-of-signal-recognition-particle-72kda-increases-radiosensitivity
#19
Remko Prevo, Gaganpreet S Tiwana, Timothy S Maughan, Francesca M Buffa, W Gillies McKenna, Geoff S Higgins
The identification of genetic determinants that underpin tumor radioresistance can help the development of targeted radiosensitizers or aid personalization of radiotherapy treatment. Here we identify signal recognition particle 72kDa (SRP72) as a novel gene involved in radioresistance. Knockdown of SRP72 resulted in significant radiosensitization of HeLa (cervical), PSN-1 (pancreatic), and T24 (bladder), BT-549 (breast) and MCF7 (breast) tumor lines as measured by colony formation assays. SRP72 depletion also resulted in the radiosensitization of normal lung fibroblast cell lines (HFL1 and MRC-5), demonstrating that the effect is not restricted to tumor cells...
June 3, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/28450809/patm-and-%C3%AE-h2ax-are-effective-radiation-biomarkers-in-assessing-the-radiosensitivity-of-12-c-6-in-human-tumor-cells
#20
Jin Zhao, Zhong Guo, Shuyan Pei, Lei Song, Chenjing Wang, Jianxiu Ma, Long Jin, Yanqing Ma, Renke He, Jianbin Zhong, Ying Ma, Hong Zhang
BACKGROUND: Tumour radiosensitivity would be particularly useful in optimizing the radiation dose during radiotherapy. The aim of the current study was to evaluate the potential value of phosphorylated H2AX (γH2AX) and ATM (pATM) in assessing (12)C(6+) radiosensitivity of tumour cells. METHODS: Human cervical carcinoma HeLa cells, hepatoma HepG2 cells, and mucoepidermoid carcinoma MEC-1 cells were irradiated with different doses of (12)C(6+). The survival fraction was assayed with clonogenic survival method and the foci of γH2AX and pATM was visualized using immunocytochemical methods...
2017: Cancer Cell International
keyword
keyword
56070
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"