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https://www.readbyqxmd.com/read/27931818/evaluation-of-the-pigret-assay-as-a-short-term-test-using-a-single-dose-of-diethylnitrosamine
#1
Kunio Wada, Risako Nishino, Tomoki Fukuyama, Kyomu Matsumoto
The PIGRET assay, which was developed as the Pig-a assay in reticulocytes, can detect mutagenicity of compounds earlier than the Pig-a assay in total red blood cells (RBC; RBC Pig-a assay). The usefulness of the PIGRET assay as a short-term test has been confirmed in a collaborative study in Japan with 24 chemicals. One of these chemicals, diethylnitrosamine (DEN), which mainly induces liver tumors in both sexes of rats, was tested. To determine the appropriate doses, DEN was dissolved in physiological saline and administered orally with a single dose to male 8-week-old Sprague-Dawley rats in a preliminary dose-range finding study...
November 15, 2016: Mutation Research
https://www.readbyqxmd.com/read/27931811/the-pigret-assay-a-method-for-measuring-pig-a-gene-mutation-in-reticulocytes-is-reliable-as-a-short-term-in-vivo-genotoxicity-test-summary-of-the-mms-jems-collaborative-study-across-16-laboratories-using-24-chemicals
#2
Takafumi Kimoto, Katsuyoshi Horibata, Daishiro Miura, Satsuki Chikura, Yuki Okada, Akiko Ukai, Satoru Itoh, Shiho Nakayama, Hisakazu Sanada, Naomi Koyama, Shigeharu Muto, Yoshifumi Uno, Mika Yamamoto, Yuta Suzuki, Takayuki Fukuda, Ken Goto, Kunio Wada, Takahiro Kyoya, Miyuki Shigano, Hironao Takasawa, Shuichi Hamada, Hideki Adachi, Yasuaki Uematsu, Eri Tsutsumi, Hisako Hori, Ryuta Kikuzuki, Yosuke Ogiwara, Ikuma Yoshida, Akihisa Maeda, Kazunori Narumi, Yohei Fujiishi, Takeshi Morita, Masami Yamada, Masamitsu Honma
The in vivo mutation assay using the X-linked phosphatidylinositol glycan class A gene (Pig-a in rodents, PIG-A in humans) is a promising tool for evaluating the mutagenicity of chemicals. Approaches for measuring Pig-a mutant cells have focused on peripheral red blood cells (RBCs) and reticulocytes (RETs) from rodents. The recently developed PIGRET assay is capable of screening >1×10(6) RETs for Pig-a mutants by concentrating RETs in whole blood prior to flow cytometric analysis. Additionally, due to the characteristics of erythropoiesis, the PIGRET assay can potentially detect increases in Pig-a mutant frequency (MF) sooner after exposure compared with a Pig-a assay targeting total RBCs (RBC Pig-a assay)...
November 15, 2016: Mutation Research
https://www.readbyqxmd.com/read/27931807/measuring-reproducibility-of-dose-response-data-for-the-pig-a-assay-using-covariate-benchmark-dose-analysis
#3
George E Johnson, Mika Yamamoto, Yuta Suzuki, Hideki Adachi, Takahiro Kyoya, Hironao Takasawa, Katsuyoshi Horibata, Eri Tsutsumi, Kunio Wada, Ryuta Kikuzuki, Ikuma Yoshida, Takafumi Kimoto, Akihisa Maeda, Kazunori Narumi
The reproducibility of the in vivo Pig-a gene mutation test system was assessed across 13 different Japanese laboratories. In each laboratory rats were exposed to the same dosing regimen of N-nitroso-N-ethylurea (ENU), and red blood cells (RBCs) and reticulocytes (RETs) were collected for mutant phenotypic analysis using flow cytometry. Mutant frequency dose response data were analysed using the PROAST benchmark dose (BMD) statistical package. Laboratory was used as a covariate during the analysis to allow all dose responses to be analysed at the same time, with conserved shape parameters...
November 15, 2016: Mutation Research
https://www.readbyqxmd.com/read/27927603/lysophosphatidic-acid-signaling-through-its-receptor-initiates-profibrotic-epithelial-cell-fibroblast-communication-mediated-by-epithelial-cell-derived-connective-tissue-growth-factor
#4
Norihiko Sakai, Jerold Chun, Jeremy S Duffield, David Lagares, Takashi Wada, Andrew D Luster, Andrew M Tager
The expansion of the fibroblast pool is a critical step in organ fibrosis, but the mechanisms driving expansion remain to be fully clarified. We previously showed that lysophosphatidic acid (LPA) signaling through its receptor LPA1 expressed on fibroblasts directly induces the recruitment of these cells. Here we tested whether LPA-LPA1 signaling drives fibroblast proliferation and activation during the development of renal fibrosis. LPA1-deficient (LPA1(-/-)) or -sufficient (LPA1(+/+)) mice were crossed to mice with green fluorescent protein expression (GFP) driven by the type I procollagen promoter (Col-GFP) to identify fibroblasts...
December 4, 2016: Kidney International
https://www.readbyqxmd.com/read/27905683/prolonged-neutropenia-due-to-antihuman-neutrophil-antigen-2-cd177-antibody-after-bone-marrow-transplantation
#5
Taizo Wada, Satoshi Miyamoto, Hiroyuki Okamoto, Yusuke Matsuda, Tomoko Toma, Kohsuke Imai, Masatoshi Takagi, Tomohiro Morio, Akihiro Yachie
We describe a patient who presented with prolonged neutropenia due to anti-human neutrophil antigen (HNA)-2 (CD177) antibody after allogeneic bone marrow transplantation. A granulocyte immunofluorescence test showed bimodal expression of antineutrophil antibody that resulted from specific binding of anti-HNA-2 to CD177(+) neutrophils from healthy donors. The patient did not respond to granulocyte colony stimulating factor, which is able to upregulate CD177 expression on neutrophils. The low percentage of CD177(+) cells in the few remaining neutrophils supports the possibility of elimination of CD177-upregulated neutrophils...
December 1, 2016: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/27904091/erratum-to-application-of-addition-cured-silicone-denture-relining-materials-to-adjust-mouthguards
#6
Shintaro Fukasawa, Hiroshi Churei, Ruman Uddin Chowdhury, Takahiro Shirako, Sharika Shahrin, Abhishekhi Shrestha, Takahiro Wada, Motohiro Uo, Hidekazu Takahashi, Toshiaki Ueno
The purposes of this study were to examine the shock absorption capability of addition-cured silicone denture relining materials and the bonding strength of addition-cured silicone denture relining materials and a commercial mouthguard material to determine its applicability to mouthguard adjustment. Two addition-cured silicone denture relining materials were selected as test materials. The impact test was applied by a free-falling steel ball. On the other hand, bonding strength was determined by a delamination test...
2016: Dental Materials Journal
https://www.readbyqxmd.com/read/27897029/a-method-for-evaluating-the-performance-of-computer-aided-detection-of-pulmonary-nodules-in-lung-cancer-ct-screening-detection-limit-for-nodule-size-and-density
#7
Hajime Kobayashi, Masaki Ohkubo, Akihiro Narita, Janaka C Marasinghe, Kohei Murao, Toru Matsumoto, Shusuke Sone, Shinichi Wada
OBJECTIVES: We propose the application of virtual nodules to evaluate the performance of computer-aided detection (CAD) of lung nodules in cancer screening using low-dose computed tomography (CT). METHODS: The virtual nodules were generated based on the spatial resolution measured for a CT system used in an institution providing cancer screening and were fused into clinical lung images obtained at that institution, allowing site-specificity. First, we validated the virtual nodules as an alternative to artificial nodules inserted into a phantom...
November 29, 2016: British Journal of Radiology
https://www.readbyqxmd.com/read/27890733/differences-in-the-onset-mode-of-ventricular-tachyarrhythmia-between-patients-with-j-wave-in-anterior-leads-versus-inferolateral-leads
#8
Tsukasa Kamakura, Mitsuru Wada, Kohei Ishibashi, Yuko Y Inoue, Koji Miyamoto, Hideo Okamura, Satoshi Nagase, Takashi Noda, Takeshi Aiba, Satoshi Yasuda, Wataru Shimizu, Shiro Kamakura, Kengo Kusano
BACKGROUND: The pathophysiological mechanism of J wave in anterior leads (A-leads) and inferolateral leads (L-leads) remains unclear. OBJECTIVE: We investigated the onset mode and circadian distribution of ventricular tachyarrhythmia (VTA) episodes in patients with early repolarization syndrome (ERS) and Brugada syndrome (BrS). METHODS: The study enrolled 35 ERS patients and 52 type 1 BrS patients with spontaneous ventricular fibrillation who were divided into four groups: ERS(A+L) (n=15), ERS patients who had non-type 1 Brugada-pattern electrocardiogram (ECG) in any A-leads (2(nd) to 4(th) intercostal spaces) in control and/or after drug provocation tests; ERS(L) (n=20), ERS with J wave only in L-leads; BrS(A) (n=24), BrS without J wave in L-leads; and, BrS(A+L) (n=28), BrS with J wave in L-leads...
November 23, 2016: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/27885819/annual-banned-substance-review-analytical-approaches-in-human-sports-drug-testing
#9
Mario Thevis, Tiia Kuuranne, Hans Geyer, Wilhelm Schänzer
There has been an immense amount of visibility of doping issues on the international stage over the past 12 months and the complexity of doping controls has been reiterated on various occasions. Hence, analytical test methods continuously being updated, expanded, and improved to provide specific, sensitive, and comprehensive test results in line with the World Anti-Doping Agency's (WADA) 2016 Prohibited List represent one of several critical cornerstones of doping controls. This enterprise necessitates expediting the (combined) exploitation of newly generated information on novel and/or superior target analytes for sports drug testing assays, drug elimination profiles, alternative test matrices, and recent advances in instrumental developments...
November 25, 2016: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27883208/the-association-between-dentition-status-and-sarcopenia-in-japanese-adults-aged-%C3%A2-75-years
#10
Masanori Iwasaki, Yumi Kimura, Hiroshi Ogawa, Taizo Wada, Ryota Sakamoto, Yasuko Ishimoto, Michiko Fujisawa, Kiyohito Okumiya, Toshihiro Ansai, Hideo Miyazaki, Kozo Matsubayashi
BACKGROUND: Sarcopenia is an age-related loss of muscle mass and muscle strength or physical performance. There are limited data on the association between oral health and sarcopenia. OBJECTIVE: To test the hypothesis that impaired dentition status was associated with sarcopenia, we conducted a cross-sectional study. METHODS: A total of 272 community-dwelling Japanese adults aged ≥75 years for whom data were available from comprehensive health examinations conducted in 2015 were included in this study...
November 24, 2016: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/27869981/description-of-the-first-cases-of-zika-virus-fever-investigated-in-municipalities-of-the-brazilian-northeastern-region-2015
#11
Francieli Fontana Sutile Tardetti Fantinato, Emerson Luiz Lima Araújo, Igor Gonçalves Ribeiro, Marcia Regina de Andrade, Anna Livia de Medeiros Dantas, Jakeline Maria Trinta Rios, Odila Márcia Vinhas da Silva, Maria do Socorro da Silva, Renata Valeria Nóbrega, Daniel de Araújo Batista, Priscila Leal E Leite, Eduardo Saad, Jadher Percio, Elionardo Andrade Resende, Priscila Bochi de Souza, Marcelo Yoshito Wada
OBJECTIVE: to confirm Zika virus circulation and discard other etiological agents in an outbreak occurred in the states of Rio Grande do Norte, Maranhão and Paraíba, in May, 2015. METHODS: this is a case series descriptive study with residents in Natal-RN, Barra do Corda-MA, São Luis-MA and João Pessoa-PB, with 20 cases in each state, presenting rash, absent or mild fever and one of the following signs/symptoms: conjunctival hyperemia, arthralgia or limb edema; RT-PCR/isolation tests for Zika, enterovirus and respiratory viruses, and serology tests (dengue, rubella and parvovirus B19) were performed...
October 2016: Epidemiologia e Servicos de Saude: Revista do Sistema Unico de Saude do Brasil
https://www.readbyqxmd.com/read/27822031/effects-of-aerobic-training-combined-with-respiratory-muscle-stretching-on-the-functional-exercise-capacity-and-thoracoabdominal-kinematics-in-patients-with-copd-a-randomized-and-controlled-trial
#12
Juliano T Wada, Erickson Borges-Santos, Desiderio Cano Porras, Denise M Paisani, Alberto Cukier, Adriana C Lunardi, Celso Rf Carvalho
BACKGROUND: Patients with COPD present a major recruitment of the inspiratory muscles, predisposing to chest incoordination, increasing the degree of dyspnea and impairing their exercise capacity. Stretching techniques could decrease the respiratory muscle activity and improve their contractile capacity; however, the systemic effects of stretching remain unknown. OBJECTIVE: The aim of this study was to evaluate the effects of aerobic training combined with respiratory muscle stretching on functional exercise capacity and thoracoabdominal kinematics in patients with COPD...
2016: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/27816897/epileptic-networks-in-patients-with-bitemporal-epilepsy-the-role-of-seeg-for-the-selection-of-good-surgical-candidates
#13
REVIEW
L Di Vito, F Mauguière, H Catenoix, S Rheims, P Bourdillon, A Montavont, J Isnard
OBJECTIVE: In this study we investigated the electrical characteristics of seizures recorded by stereo-EEG (SEEG) in a cohort of patients with bitemporal lobe epilepsy (BTLE), in the attempt to verify the presence/absence of different seizure patterns and to identify good surgical candidates. METHODS: We retrospectively reviewed 14 consecutive patients with medically refractory BTLE confirmed by scalp video-EEG (VEEG) recording of bitemporal independent or non-lateralizing seizure onset...
December 2016: Epilepsy Research
https://www.readbyqxmd.com/read/27800589/endoscopic-marking-clip-with-an-ic-tag-and-receiving-antenna-to-detect-localization-during-laparoscopic-surgery
#14
Yuma Wada, Norikatsu Miyoshi, Masayuki Ohue, Masayoshi Yasui, Shiki Fujino, Akira Tomokuni, Keijirou Sugimura, Hirofumi Akita, Jeong Ho Moon, Hidenori Takahashi, Shogo Kobayashi, Takeshi Omori, Hiroshi Miyata, Yoshiyuki Fujiwara, Masahiko Yano, Masato Sakon
BACKGROUND: Conventional tattooing using India ink for preoperative marking during colonoscopy has been widely used. However, the ink used in this method can spread into the abdominal cavity and induce peritonitis. Therefore, we developed a new marking method using an endoscopic clip with an integrated circuit (IC) tag to accurately identify an objective location. We applied the novel method in laparoscopic surgery using a porcine model and also evaluated it with resected human gastrointestinal tissue...
October 31, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27780372/sweep-frequency-impedance-measures-in-young-infants-developmental-characteristics-from-birth-to-6-months
#15
Venkatesh Aithal, Joseph Kei, Carlie Driscoll, Michio Murakoshi, Hiroshi Wada
OBJECTIVE: International Journal of Audiology To study the developmental characteristics of sweep frequency impedance (SFI) measures in healthy infants from birth to 6 months. DESIGN: All infants were assessed using high-frequency tympanometry (HFT), distortion product otoacoustic emission (DPOAE) and SFI tests. SFI measures consisted of measurement of resonance frequency (RF) and mobility (ΔSPL) of the outer and middle ear. A mixed model analysis of variance was applied to the SFI data to examine the effect of age on RF and ΔSPL...
October 26, 2016: International Journal of Audiology
https://www.readbyqxmd.com/read/27776186/serum-reactive-oxygen-metabolite-levels-predict-severe-exacerbations-of-asthma
#16
Keitaro Nakamoto, Masato Watanabe, Mitsuru Sada, Toshiya Inui, Masuo Nakamura, Kojiro Honda, Hiroo Wada, Yu Mikami, Hirotaka Matsuzaki, Masafumi Horie, Satoshi Noguchi, Yasuhiro Yamauchi, Hikari Koyama, Toshiyuki Kogane, Tadashi Kohyama, Hajime Takizawa
BACKGROUND AND PURPOSE: Bronchial asthma (BA) is a chronic airway disease characterized by airway hyperresponsiveness and remodeling, which are intimately linked to chronic airway inflammation. Reactive oxygen species (ROS) such as hydrogen peroxide are generated by inflammatory cells that are involved in the pathogenesis of BA. However, the role of ROS in the management of BA patients is not yet clear. We attempted to determine the role of ROS as a biomarker in the clinical setting of BA...
2016: PloS One
https://www.readbyqxmd.com/read/27732769/longitudinal-evaluation-of-the-isotope-ratio-mass-spectrometric-data-towards-the-isotopic-module-of-the-athlete-biological-passport
#17
Daniel Jardines, Francesco Botrè, Cristiana Colamonici, Davide Curcio, Gemma Procida, Xavier de la Torre
The detection of the abuse of pseudo-endogenous steroids (testosterone and/or its precursors) is currently based on the application of the steroid module of the World Anti-Doping Agency (WADA) Athletes' Biological Passport (ABP), implemented through ADAMS. Diagnostic metabolites are monitored for every athlete and statistically evaluated with a predictive Bayesian approach. In the case of suspicious samples, the data of the ABP are confirmed and the isotope ratio mass spectrometry (IRMS) test is activated. We have previously demonstrated that IRMS enables confirmation of the non-endogenous origin of pseudo-endogenous steroids in otherwise non-suspicious samples, after a longitudinal evaluation of the ABP, even after the inclusion of additional long-term diagnostic hydroxylated metabolites, and that the delta values of the parameters obtained during the IRMS confirmation process presented much less variability compared to the parameters of the ABP...
November 2016: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27729561/evaluation-of-the-diagnostic-criteria-for-the-basic-type-of-dic-established-by-the-japanese-society-of-thrombosis-and-hemostasis
#18
Takumi Aota, Hideo Wada, Naoki Fujimoto, Yoshiki Yamashita, Takeshi Matsumoto, Kohshi Ohishi, Kei Suzuki, Hiroshi Imai, Masanobu Usui, Shuji Isaji, Toshimasa Uchiyama, Yoshinobu Seki, Naoyuki Katayama
We evaluated the diagnostic criteria for disseminated intravascular coagulation (DIC), which was published by the Japanese Society of Thrombosis and Hemostasis (JSTH), in 232 patients with suspected DIC without hematopoietic injury or infection. The diagnoses of the patients were as follows: DIC (n = 116), pre-DIC (n = 54), and non-DIC (n = 63). The efficacy of the diagnostic criteria for DIC was evaluated using a receiver operating characteristic analysis. The area under the curve and odds ratio for the global coagulation test (GCT) scores in the diagnosis of "DIC" were high, whereas those for the diagnosis of "DIC and pre-DIC" were low, suggesting that the addition of a reduced platelet count (RPC), antithrombin (AT), and soluble fibrin (SF)/thrombin AT (TAT) complex was required to diagnose DIC and pre-DIC...
October 11, 2016: Clinical and Applied Thrombosis/hemostasis
https://www.readbyqxmd.com/read/27725574/generic-selection-criteria-for-safety-and-patient-benefit-vi-comparing-the-physicochemical-and-pharmaceutical-properties-of-brand-name-generic-and-otc-felbinac-tapes
#19
Yuko Wada, Yukie Takaoka, Mitsuru Nozawa, Miho Goto, Ken-Ichi Shimokawa, Fumiyoshi Ishii
We measured the pH, water-vapor permeability, adhesive force, peeling-force, elongation rate, support flexibility, and peeling time of medicinal and over-the-counter (OTC) tape preparations containing felbinac. When measuring the pH of each preparation, Felnabion (pH 4.5) was weakly acidic, and EMEC and Tokuon (pH 7.0) were neutral. When measuring the water-vapor permeability of each preparation, that of a generic product, EMEC (380 g/m(2)/24h), was twice as high as that of a brand-name product, Seltouch (189 g/m(2)/24 h)...
October 4, 2016: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/27714988/intranasal-delivery-of-natesto%C3%A2-testosterone-gel-and-its-effects-on-doping-markers
#20
Geoffrey D Miller, Vinod Nair, M Scott Morrison, Maggie Summers, Stuart E Willick, Daniel Eichner
The laboratory profile of intranasal testosterone gel has not been previously reported from an anti-doping perspective. Because intranasal testosterone gel is newly available as a commercial product, we sought to examine the laboratory parameters following administration of this formulation, with particular attention to anti-doping guidelines. Five healthy and active male subjects were administered testosterone intranasal gel three times daily for four weeks, using a pattern of five consecutive days on, two days off...
November 2016: Drug Testing and Analysis
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