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Human bone scaffold

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https://www.readbyqxmd.com/read/29140567/human-adipose-derived-stromal-cell-isolation-methods-and-use-in-osteogenic-and-adipogenic-in-vivo-applications
#1
Elizabeth Brett, Ruth Tevlin, Adrian McArdle, Eun Young Seo, Charles K F Chan, Derrick C Wan, Michael T Longaker
Adipose tissue represents an abundant and easily accessible source of multipotent cells, which may serve as excellent building blocks for tissue engineering. This article presents a newly described protocol for isolating adipose-derived stromal cells (ASCs) from human lipoaspirate, compared to the standard protocol for harvesting ASCs established in 2001. Human ASC isolation is performed using two methods, and resultant cells are compared through cell yield, cell viability, cell proliferation and regenerative potential...
November 15, 2017: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/29127064/multifunctional-ph-sensitive-3d-scaffolds-for-treatment-and-prevention-of-bone-infection
#2
Mónica Cicuéndez, Juan C Doadrio, Ana Hernández, M Teresa Portolés, Isabel Izquierdo-Barba, María Vallet-Regí
Multifunctional-therapeutic three-dimensional (3D) scaffolds have been prepared. These biomaterials are able to destroy the S. aureus bacterial biofilm and to allow bone regeneration at the same time. The present study is focused on the design of pH sensitive 3D hierarchical meso-macroporous 3D scaffolds based on MGHA nanocomposite formed by a mesostructured glassy network with embedded hydroxyapatite nanoparticles, whose mesopores have been loaded with levofloxacin (Levo) as antibacterial agent. These 3D platforms exhibit controlled and pH-dependent Levo release, sustained over time at physiological pH (7...
November 7, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29126441/tgf%C3%AE-3-recruits-endogenous-mesenchymal-stem-cells-to-initiate-bone-regeneration
#3
Moyuan Deng, Tieniu Mei, Tianyong Hou, Keyu Luo, Fei Luo, Aijun Yang, Bo Yu, Hao Pang, Shiwu Dong, Jianzhong Xu
BACKGROUND: The recruitment of a sufficient number of endogenous mesenchymal stem cells (MSCs) is the first stage of in-situ tissue regeneration. Transforming growth factor beta-3 (TGFβ3) could recruit stem or progenitor cells and endothelial cells to participate in tissue regeneration. However, the mechanism of TGFβ3 recruiting MSCs toward bone regeneration has remained obscure. METHODS: We estimated the promigratory property of TGFβ3 on human bone marrow MSCs (hBMSCs) cocultured with the vascular cells (human umbilical artery smooth muscle cells or human umbilical vein endothelial cells) or not by Transwell assay...
November 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29124078/demineralized-bone-matrix-to-augment-tendon-bone-healing-a-systematic-review
#4
Adam T Hexter, Catherine Pendegrass, Fares Haddad, Gordon Blunn
Background: Following injury to the rotator cuff and anterior cruciate ligament, a direct enthesis is not regenerated, and healing occurs with biomechanically inferior fibrous tissue. Demineralized bone matrix (DBM) is a collagen scaffold that contains growth factors and is a promising biological material for tendon and ligament repair because it can regenerate a direct fibrocartilaginous insertion via endochondral ossification. Purpose: To provide a comprehensive review of the literature investigating the use of DBM to augment tendon-bone healing in tendon repair and anterior cruciate ligament reconstruction (ACLR)...
October 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29123118/substrate-microarchitecture-shapes-the-paracrine-crosstalk-of-stem-cells-with-endothelial-cells-and-osteoblasts
#5
Francisco Martín-Saavedra, Lara Crespo, Clara Escudero-Duch, Laura Saldaña, Enrique Gómez-Barrena, Nuria Vilaboa
We examined the hypothesis that substrate microarchitecture regulates the crosstalk between human mesenchymal stem cells (hMSC) and cell types involved in bone regeneration. Compared with polyester flat substrates having uniformly distributed homogenous pores (2D), three-dimensional polystyrene substrates with randomly oriented and interconnected pores of heterogeneous size (3D) stimulated the stromal secretion of IGF-1 while lessened the production of VEGFR-1, MCP-1 and IL-6. The medium conditioned by hMSC cultured in 3D substrates stimulated tube formation by human endothelial cells (hEC) to a higher extent than medium from 2D cultures...
November 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29122713/fabrication-and-characterization-of-nanobiocomposite-scaffold-of-zein-chitosan-nanohydroxyapatite-prepared-by-freeze-drying-method-for-bone-tissue-engineering
#6
Zeinab Shahbazarab, Abbas Teimouri, Alireza Najafi Chermahini, Mohammad Azadi
In this investigation, porous composite scaffolds were prepared using a freeze-drying procedure by mixing zein (ZN), chitosan (CS) and nanohydroxyapatite (nHAp) in different inorganic/organic weight ratios. The gained nanocomposite scaffolds were studied using X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and N2-adsorption-desorption technique. Also, swelling, porosity, mechanical properties, biomineralization capability, degradation, cell attachment, and cell viability of the composite scaffolds were studied...
November 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29119879/magnesium-and-zinc-borate-enhance-osteoblastic-differentiation-of-stem-cells-from-human-exfoliated-deciduous-teeth-in-vitro
#7
Yao-Jen Liu, Wen-Ta Su, Po-Hung Chen
Various biocompatible and biodegradable scaffolds blended with biochemical signal molecules with adequate osteoinductive and osteoconductive properties have attracted significant interest in hard tissue engineering regeneration. We evaluated the distinct effects of magnesium borate, zinc borate, and boric acid blended into chitosan scaffold for osteogenic differentiation of stem cells from exfoliated deciduous teeth. Stem cells from exfoliated deciduous teeth cells are a potential source of functional osteoblasts for applications in bone tissue engineering, but the efficiency of osteoblastic differentiation is low, thereby significantly limiting their clinical applications...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29113502/osteogenic-and-antibacterial-properties-of-vancomycin-laden-mesoporous-bioglass-plga-composite-scaffolds-for-bone-regeneration-in-infected-bone-defects
#8
Tao Cheng, Haiyun Qu, Guoyou Zhang, Xianlong Zhang
Chronic osteomyelitis and infected bone defects are substantial challenges faced by orthopaedic surgeons. In this study, vancomycin was loaded into mesoporous bioactive glass (MBG) to form a local antibiotic delivery system and then a bone tissue-engineering scaffold combining MBG and poly-(L-lactic-co-glycolic acid) (PLGA) was prepared by freeze-drying fabrication. In vitro degradation and water contact angle analysis suggested that the MBG-incorporated PLGA scaffold exhibited controlled degradability, stabilizing the pH of the surrounding environment and improved the hydrophilicity...
November 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29105943/characterization-of-printed-pla-scaffolds-for-bone-tissue-engineering
#9
Agathe Grémare, Vera Guduric, Reine Bareille, Valérie Heroguez, Simon Latour, Nicolas L'Heureux, Jean-Christophe Fricain, Sylvain Catros, Damien Le Nihouannen
Autografts remain the gold standard for orthopedic transplantations. However, to overcome its limitations, bone tissue engineering proposes new strategies. This includes the development of new biomaterials such as synthetic polymers, to serve as scaffold for tissue production. The objective of this present study was to produce poly(lactic) acid (PLA) scaffolds of different pore size using fused deposition modeling (FDM) technique and to evaluate their physicochemical and biological properties. Structural, chemical, mechanical and biological characterizations were performed...
November 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29104917/prevention-of-oxygen-inhibition-of-polyhipe-radical-polymerization-using-a-thiol-based-crosslinker
#10
Michael E Whitely, Jennifer L Robinson, Melissa C Stuebben, Hannah A Pearce, Madison A P McEnery, Elizabeth Cosgriff-Hernandez
Polymerized high internal phase emulsions (polyHIPEs) are highly porous constructs currently under investigation as tissue engineered scaffolds. We previously reported on the potential of redox-initiated polyHIPEs as injectable bone grafts that space fill irregular defects with improved integration and rapid cure. Upon subsequent investigation, the radical-initiated cure of these systems rendered them susceptible to oxygen inhibition with an associated increase in uncured macromer in the clinical setting. In the current study, polyHIPEs with increased resistance to oxygen inhibition were fabricated utilizing a tetrafunctional thiol, pentaerythritol tetrakis(3-mercaptoproprionate), and the biodegradable macromer, propylene fumarate dimethacrylate...
March 13, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29104482/human-cartilage-engineering-in-an-in-vitro-repair-model-using-collagen-scaffolds-and-mesenchymal-stromal-cells
#11
Clara Sanjurjo-Rodríguez, Rocío Castro-Viñuelas, Tamara Hermida-Gómez, Isaac Manuel Fuentes-Boquete, Francisco Javier de Toro, Francisco Javier Blanco, Silvia María Díaz-Prado
The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made in human cartilage biopsies. Injured samples were pre-treated with interleukin 1β (IL1β) for 24 h; also, samples were not pre-treated. hBMSCs were seeded on different types of collagen scaffolds. The resulting constructs were placed into the lesions, and the biopsies were cultured for 2 months in chondrogenic medium...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29102342/bioengineered-lungs-a-challenge-and-an-opportunity
#12
Ramon Farré, Jordi Otero, Isaac Almendros, Daniel Navajas
Lung biofabrication is a new tissue engineering and regenerative development aimed at providing organs for potential use in transplantation. Lung biofabrication is based on seeding cells into an acellular organ scaffold and on culturing them in an especial purpose bioreactor. The acellular lung scaffold is obtained by decellularizing a non-transplantable donor lung by means of conventional procedures based on application of physical, enzymatic and detergent agents. To avoid immune recipient's rejection of the transplanted bioengineered lung, autologous bone marrow/adipose tissue-derived mesenchymal stem cells, lung progenitor cells or induced pluripotent stem cells are used for biofabricating the bioengineered lung...
November 1, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/29098767/silicon-bioceramic-loaded-with-vancomycin-stimulates-bone-tissue-regeneration
#13
Angel Manchón, Mohammad H Alkhraisat, Carmen Rueda-Rodriguez, Concepción Pintado, J C Prados-Frutos, Jesus Torres, Enrique Lopez Cabarcos
Porous ceramics doped with silicon and pure β-TCP were analyzed in terms of internal microstructure, cell behavior, and the percentage of newly formed bone. Additionally the materials were tested to determine which of the two had better properties to load and release vancomycin hydrochloride. Internal pore distribution and porosity were determined through high pressure mercury porosimetry and the specific surface area was measured by the Brunauer Emmet-Teller method. The proliferation and viability of the human osteoblast-like cell line MG-63 was studied to validate both materials...
November 3, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29095911/correction-human-decellularized-bone-scaffolds-from-aged-donors-show-improved-osteoinductive-capacity-compared-to-young-donor-bone
#14
Christopher A Smith, Tim N Board, Paul Rooney, Mark J Eagle, Stephen M Richardson, Judith A Hoyland
[This corrects the article DOI: 10.1371/journal.pone.0177416.].
2017: PloS One
https://www.readbyqxmd.com/read/29094460/hydrogel-fiber-conductive-scaffold-for-bone-tissue-engineering
#15
Sajedeh Khorshidi, Akbar Karkhaneh
Hydrogel/fiber composites have emerged as compelling scaffolds for regeneration purposes. Any bio-related modification or feature may endow more regenerative functionality to these composites. In the present study, a hydrogel/fiber scaffold possessing electrical conductivity in both phases, hydrogel and fiber, has been prepared and evaluated. Fiber component possessed electrical conductivity due to the presence of polyaniline (PANi) and hydrogel fraction thanks to the presence of graphene nanoparticles. PANi based fibers were processed via electrospinning and transformed into a three-dimensional structure through ultrasonication...
November 1, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29092692/characterization-of-multi-layered-tissue-engineered-human-alveolar-bone-and-gingival-mucosa
#16
Thafar Almela, Sarmad Al-Sahaf, Robert Bolt, Ian Brook, K Moharamzadeh
Advances in tissue engineering have permitted assembly of multi-layered composite tissue constructs for potential applications in the treatment of combined hard and soft tissue defects and as an alternative in vitro test model to animal experimental systems. The aim of this study was to develop and characterize a novel three-dimensional combined human alveolar bone and gingival mucosal model based on primary cells isolated from the oral tissues. Bone component of the model was engineered by seeding primary human alveolar osteoblasts (HAOBs) into a hydroxyapatite/tricalcium phosphate (HA/TCP) scaffold and culturing in a spinner bioreactor...
November 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29090300/heterogeneous-and-self-organizing-mineralization-of-bone-matrix-promoted-by-hydroxyapatite-nanoparticles
#17
G Campi, F Cristofaro, G Pani, M Fratini, B Pascucci, P A Corsetto, B Weinhausen, A Cedola, A M Rizzo, L Visai, G Rea
The mineralization process is crucial to the load-bearing characteristics of the bone extracellular matrix. In this work, we have studied the spatiotemporal dynamics of mineral deposition by human bone marrow mesenchymal stem cells differentiating toward osteoblasts promoted by the presence of exogenous hydroxyapatite nanoparticles. At the molecular level, the added nanoparticles positively modulated the expression of bone-specific markers and enhanced calcified matrix deposition during osteogenic differentiation...
November 1, 2017: Nanoscale
https://www.readbyqxmd.com/read/29078325/necroptosis-controls-net-generation-and-mediates-complement-activation-endothelial-damage-and-autoimmune-vasculitis
#18
Adrian Schreiber, Anthony Rousselle, Jan Ulrich Becker, Anne von Mässenhausen, Andreas Linkermann, Ralph Kettritz
Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) constitutes life-threatening autoimmune diseases affecting every organ, including the kidneys, where they cause necrotizing crescentic glomerulonephritis. ANCA activates neutrophils and activated neutrophils damage the endothelium, leading to vascular inflammation and necrosis. Better understanding of neutrophil-mediated AAV disease mechanisms may reveal novel treatment strategies. Here we report that ANCA induces neutrophil extracellular traps (NETs) via receptor-interacting protein kinase (RIPK) 1/3- and mixed-lineage kinase domain-like (MLKL)-dependent necroptosis...
October 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29077014/mesoporous-bioactive-glass-functionalized-3d-ti-6al-4v-scaffolds-with-improved-surface-bioactivity
#19
Xiaotong Ye, Sander Leeflang, Chengtie Wu, Jiang Chang, Jie Zhou, Zhiguang Huan
Porous Ti-6Al-4V scaffolds fabricated by means of selective laser melting (SLM), having controllable geometrical features and preferable mechanical properties, have been developed as a class of biomaterials that hold promising potential for bone repair. However, the inherent bio-inertness of the Ti-6Al-4V alloy as the matrix of the scaffolds results in a lack in the ability to stimulate bone ingrowth and regeneration. The aim of the present study was to develop a bioactive coating on the struts of SLM Ti-6Al-4V scaffolds in order to add the desired surface osteogenesis ability...
October 27, 2017: Materials
https://www.readbyqxmd.com/read/29073831/pthrp-gradients-affect-the-progression-of-mesenchymal-stem-cell-chondrogenesis-and-hypertrophy
#20
Niamh Fahy, Oliver Gardner, Mauro Alini, Martin James Stoddart
INTRODUCTION: Mesenchymal stem cells (MSCs) are considered a promising cell source for cartilage repair strategies due to their chondrogenic differentiation potential. However, their in-vitro tendency to progress towards hypertrophy limits their clinical use. This unfavourable result may be due to the fact that MSCs used in tissue engineering approaches are all at the same developmental stage, and have lost crucial spatial and temporal signalling cues. In this study, we sought to investigate the effect of a spatial parathyroid hormone-related protein (PTHrP) signalling gradient on the chondrogenic differentiation of MSCs and progression to hypertrophy...
October 26, 2017: Tissue Engineering. Part A
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