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Human bone scaffold

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https://www.readbyqxmd.com/read/28087448/fabrication-and-characterization-of-electrospun-cellulose-nano-hydroxyapatite-nanofibers-for-bone-tissue-engineering
#1
Chenghong Ao, Yan Niu, Ximu Zhang, Xu He, Wei Zhang, Canhui Lu
Nanofibrous scaffolds from cotton cellulose and nano-hydroxyapatite (nano-HA) were electrospun for bone tissue engineering. The solution properties of cellulose/nano-HA spinning dopes and their associated electrospinnability were characterized. Morphological, thermal and mechanical properties of the electrospun cellulose/nano-HA nanocomposite nanofibers (ECHNN) were measured and the biocompatibility of ECHNN with human dental follicle cells (HDFCs) was evaluated. Scanning electron microscope (SEM) images indicated that the average diameter of ECHNN increased with a higher nano-HA loading and the fiber diameter distributions were well within the range of natural ECM (extra cellular matrix) fibers (50-500nm)...
January 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28078846/mir-2861-is-involved-in-osteogenic-commitment-of-human-periodontal-ligament-stem-cells-grown-onto-3d-scaffold
#2
F Diomede, I Merciaro, S Martinotti, M F X B Cavalcanti, S Caputi, E Mazzon, O Trubiani
miR-2861 endorsing osteoblast differentiation through the overexpression of Runt-related transcription factor 2 (RUNX2) protein has been recently described. In this study we evaluated: the performance of living construct, composed by human Periodontal Ligament Stem Cells (hPDLSCs) and 3D scaffold (EXg), and the behaviour of miR-2861/RUNX2 expression pathway on the osteogenic commitment. Human PDLSCs were seeded with and without EXg scaffold and cultured under basal and osteogenic conditions. Morphological features, adhesiveness and differentiation abilities were analysed using scanning electron and confocal laser scanning microscopy...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28078807/traumatized-muscle-derived-multipotent-progenitor-cells-recruit-endothelial-cells-through-vascular-endothelial-growth-factor-a-action
#3
Heidi R H Supanc, Shannon Gorman, Rocky S Tuan
Traumatized muscle, such as that debrided from blast injury sites, is considered a promising and convenient tissue source for multipotent progenitor cells (MPCs), a population of adult mesenchymal stem cell (MSC)-like cells. The present study aimed to assess the regenerative therapeutic potential of human traumatized muscle-derived MPCs, e.g., for injury repair in the blast-traumatized extremity, by comparing their pro-angiogenic potential in vitro and capillary recruitment activity in vivo to those of MSCs isolated from human bone marrow, a widely-used tissue source...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28074350/a-comparative-study-of-the-bone-regenerative-effect-of-chemically-modified-rna-encoding-bmp-2-or-bmp-9
#4
Behnoush Khorsand, Satheesh Elangovan, Liu Hong, Alexander Dewerth, Michael S D Kormann, Aliasger K Salem
Employing cost-effective biomaterials to deliver chemically modified ribonucleic acid (cmRNA) in a controlled manner addresses the high cost, safety concerns, and lower transfection efficiency that exist with protein and gene therapeutic approaches. By eliminating the need for nuclear entry, cmRNA therapeutics can potentially overcome the lower transfection efficiencies associated with non-viral gene delivery systems. Here, we investigated the osteogenic potential of cmRNA-encoding BMP-9, in comparison to cmRNA-encoding BMP-2...
January 10, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28073305/human-amniotic-mesenchymal-stromal-cells-as-favourable-source-for-cartilage-repair
#5
Emma Muinos-López, Tamara Hermida-Gómez, Isaac Fuentes-Boquete, Francisco Javier de Toro-Santos, Francisco J Blanco, Silvia María Díaz-Prado
INTRODUCTION: Localized trauma-derived breakdown of the hyaline articular cartilage may progress towards osteoarthritis, a degenerative condition characterized by total loss of articular cartilage and joint function. Tissue engineering technologies encompass several promising approaches with high therapeutic potential for the treatment of these focal defects. However, most of the research in tissue engineering is focused on potential materials and structural cues, while little attention is directed to the most appropriate source of cells endowing these materials...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28073075/freeform-extrusion-fabrication-of-titanium-fiber-reinforced-13-93-bioactive-glass-scaffolds
#6
Albin Thomas, Krishna C R Kolan, Ming C Leu, Gregory E Hilmas
Although implants made with bioactive glass have shown promising results for bone repair, their application in repairing load-bearing long bone is limited due to their poor mechanical properties in comparison to human bone. This work investigates the freeform extrusion fabrication of bioactive silicate 13-93 glass scaffolds reinforced with titanium (Ti) fibers. A composite paste prepared with 13-93 glass and Ti fibers (~16µm in diameter and lengths varying from ~200µm to ~2 mm) was extruded through a nozzle to fabricate scaffolds (0-90° filament orientation pattern) on a heated plate...
December 31, 2016: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28071988/braided-and-stacked-electrospun-nanofibrous-scaffolds-for-tendon-and-ligament-tissue-engineering
#7
Benjamin B Rothrauff, Brian B Lauro, Guang Yang, Richard E Debski, Volker Musahl, Rocky Tuan
Tendon and ligament injuries are a persistent orthopaedic challenge given their poor innate healing capacity. Non-woven electrospun nanofibrous scaffolds composed of polyesters have been used to mimic the mechanics and topographical cues of native tendons and ligaments. However, non-woven nanofibers have several limitations that prevent broader clinical application, including poor cell infiltration as well as tensile and suture-retention strengths that are inferior to native tissues. In this study, multilayered scaffolds of aligned electrospun nanofibers of two designs - stacked or braided - were fabricated...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28071596/3d-bioprinting-of-gelma-scaffolds-triggers-mineral-deposition-by-primary-human-osteoblasts
#8
Christine McBeth, Jasmin Lauer, Michael Ottersbach, Jennifer Campbell, Andre Sharon, Alexis F Sauer-Budge
Due to its relatively low level of antigenicity and high durability, titanium has successfully been used as the major material for biological implants. However, because the typical interface between titanium and tissue precludes adequate transmission of load into the surrounding bone, over time, load-bearing implants tend to loosen and revision surgeries are required. Osseointegration of titanium implants requires presentation of both biological and mechanical cues that promote attachment of and trigger mineral deposition by osteoblasts...
January 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28070691/biological-characteristic-effects-of-human-dental-pulp-stem-cells-on-poly-%C3%AE%C2%B5-caprolactone-biphasic-calcium-phosphate-fabricated-scaffolds-using-modified-melt-stretching-and-multilayer-deposition
#9
Natkrita Wongsupa, Thongchai Nuntanaranont, Suttatip Kamolmattayakul, Nuttawut Thuaksuban
Craniofacial bone defects such as alveolar cleft affect the esthetics and functions that need bone reconstruction. The advanced techniques of biomaterials combined with stem cells have been a challenging role for maxillofacial surgeons and scientists. PCL-coated biphasic calcium phosphate (PCL-BCP) scaffolds were created with the modified melt stretching and multilayer deposition (mMSMD) technique and merged with human dental pulp stem cells (hDPSCs) to fulfill the component of tissue engineering for bone substitution...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28069556/regeneration-of-bone-using-nanoplex-delivery-of-fgf-2-and-bmp-2-genes-in-diaphyseal-long-bone-radial-defects-in-a-diabetic-rabbit-model
#10
Behnoush Khorsand, Nate Nicholson, Anh-Vu Do, John E Femino, James A Martin, Emily Petersen, Brian Guetschow, Douglas C Fredericks, Aliasger K Salem
Bone fracture healing impairment related to systemic diseases such as diabetes can be addressed by growth factor augmentation. We previously reported that growth factors such as fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) work synergistically to encourage osteogenesis in vitro. In this report, we investigated if BMP-2 and FGF-2 together can synergistically promote bone repair in a leporine model of diabetes mellitus, a condition that is known to be detrimental to union. We utilized two kinds of plasmid DNA encoding either BMP-2 or FGF-2 formulated into polyethylenimine (PEI) complexes...
January 7, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28068621/formation-of-porous-hpcl-lpcl-ha-scaffolds-with-supercritical-co2-gas-foaming-method
#11
M Zahedi Moghadam, Sh Hassanajili, F Esmaeilzadeh, M Ayatollahi, M Ahmadi
Scaffold is a 3D porous structure that is made of different materials, such as synthetic and natural polymers. It plays the role of a synthetic extracellular matrix and permits adhesion, proliferation and differentiation of the cells. Porosity and pore size are the important factors for any 3D scaffold used in bone tissue engineering. In this study, porous scaffolds were prepared by adding hydroxyapatite (HA) nanoparticles as filler to the polymeric matrix of polycaprolactone (PCL) blends with two different molecular weight by using supercritical CO2 (ScCO2) foaming method...
January 3, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28068081/peptide-decorated-nanofibrous-niche-augments-in-vitro-directed-osteogenic-conversion-of-human-pluripotent-stem-cells
#12
Yi Deng, Yuanyi Yang, Shicheng Wei
Realization of clinical potential of human pluripotent stem cells (hPSCs) in bone regenerative medicine requires development of simple and safe biomaterials for expansion of hPSCs followed by directing their lineage commitment to osteoblasts. In the present study, a chemically defined peptide-decorated polycaprolactone (PCL) nanofibrous microenvironment was prepared through electrospinning technology and subsequent conjugation with vitronectin peptide to promote the culture and osteogenic potential of hPSCs in vitro...
January 9, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28067666/versatile-humanized-niche-model-enables-study-of-normal-and-malignant-human-hematopoiesis
#13
Ander Abarrategi, Katie Foster, Ashley Hamilton, Syed A Mian, Diana Passaro, John Gribben, Ghulam Mufti, Dominique Bonnet
The BM niche comprises a tightly controlled microenvironment formed by specific tissue and cells that regulates the behavior of hematopoietic stem cells (HSCs). Here, we have provided a 3D model that is tunable in different BM niche components and useful, both in vitro and in vivo, for studying the maintenance of normal and malignant hematopoiesis. Using scaffolds, we tested the capacity of different stromal cell types to support human HSCs. Scaffolds coated with human mesenchymal stromal cells (hMSCs) proved to be superior in terms of HSC engraftment and long-term maintenance when implanted in vivo...
January 9, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28060874/salmon-dna-accelerates-bone-regeneration-by-inducing-osteoblast-migration
#14
Ayako Sato, Hiroshi Kajiya, Nana Mori, Hironobu Sato, Tadao Fukushima, Hirofumi Kido, Jun Ohno
The initial step of bone regeneration requires the migration of osteogenic cells to defective sites. Our previous studies suggest that a salmon DNA-based scaffold can promote the bone regeneration of calvarial defects in rats. We speculate that the salmon DNA may possess osteoinductive properties, including the homing of migrating osteogenic cells. In the present study, we investigated the influence of the salmon DNA on osteoblastic differentiation and induction of osteoblast migration using MG63 cells (human preosteoblasts) in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28060333/mesenchymal-stromal-cell-culture-and-delivery-in-autologous-conditions-a-smart-approach-for-orthopedic-applications
#15
Luisa Trombi, Serena Danti, Sara Savelli, Stefania Moscato, Delfo D'Alessandro, Claudio Ricci, Stefano Giannotti, Mario Petrini
Human Mesenchymal Stromal Cells (hMSCs) are cultured in vitro with different media. Limits on their use in clinical settings, however, mainly depend on potential biohazard and inflammation risks exerted by xenogeneic nutrients for their culture. Human derivatives or recombinant materials are the first choice candidates to reduce these reactions. Therefore, culture supplements and materials of autologous origin represent the best nutrients and the safest products. Here, we describe a new protocol for the isolation and culture of bone marrow hMSCs in autologous conditions - namely, patient-derived serum as a supplement for the culture medium and fibrin as a scaffold for hMSC administration...
December 8, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28056713/three-dimensional-printed-bone-scaffolds-the-role-of-nano-micro-hydroxyapatite-particles-on-the-adhesion-and-differentiation-of-human-mesenchymal-stem-cells
#16
Marco Domingos, Antonio Gloria, Jorge Coelho, Paulo Bartolo, Joaquim Ciurana
Bone tissue engineering is strongly dependent on the use of three-dimensional scaffolds that can act as templates to accommodate cells and support tissue ingrowth. Despite its wide application in tissue engineering research, polycaprolactone presents a very limited ability to induce adhesion, proliferation and osteogenic cell differentiation. To overcome some of these limitations, different calcium phosphates, such as hydroxyapatite and tricalcium phosphate, have been employed with relative success. This work investigates the influence of nano-hydroxyapatite and micro-hydroxyapatite (nHA and mHA, respectively) particles on the in vitro biomechanical performance of polycaprolactone/hydroxyapatite scaffolds...
December 1, 2016: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/28054585/osseous-differentiation-of-human-fat-tissue-grafts-from-tissue-engineering-to-tissue-differentiation
#17
Maryna Bondarava, Chiara Cattaneo, Bin Ren, Wolfgang E Thasler, Volkmar Jansson, Peter E Müller, Oliver B Betz
Conventional bone tissue engineering approaches require isolation and in vitro propagation of autologous cells, followed by seeding on a variety of scaffolds. Those protracted procedures impede the clinical applications. Here we report the transdifferentiation of human fat tissue fragments retrieved from subcutaneous fat into tissue with bone characteristics in vitro without prior cell isolation and propagation. 3D collagen-I cultures of human fat tissue were cultivated either in growth medium or in osteogenic medium (OM) with or without addition of Bone Morphogenetic Proteins (BMPs) BMP-2, BMP-7 or BMP-9...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28040084/do-skeletal-muscle-mscs-in-humans-contribute-to-bone-repair-a-systematic-review
#18
Heather Owston, Peter V Giannoudis, Elena Jones
Mesenchymal stem cells (MSC) from bone marrow and periosteum are known to be heavily involved in fracture repair and bone regeneration is thought to be impaired when the surrounding skeletal muscle is damaged. Recent literature from mouse in vivo models suggest that cells originating from skeletal muscle can occupy a fracture callus during open fracture repair when periosteum is compromised. This systematic review set out to ascertain whether there are MSCs residing in human skeletal muscle and whether cells from human skeletal muscle are capable of forming bone in vitro and in vivo...
December 2016: Injury
https://www.readbyqxmd.com/read/28040083/a-novel-aragonite-based-scaffold-for-osteochondral-regeneration-early-experience-on-human-implants-and-technical-developments
#19
Elizaveta Kon, Dror Robinson, Peter Verdonk, Matej Drobnic, Jenel Mariano Patrascu, Oliver Dulic, Gordon Gavrilovic, Giuseppe Filardo
INTRODUCTION: Chondral and osteochondral lesions represent a debilitating disease. Untreated lesions remain a risk factor for more extensive joint damage. The objective of this clinical study is to evaluate safety and early results of an aragonite-based scaffold used for osteochondral unit repair, by analysing both clinical outcome and MRI results, as well as the benefits of the procedure optimization through novel tapered shaped implants. METHODS: A crystalline aragonite bi-phasic scaffold was implanted in patients affected by focal chondral-osteochondral knee lesions of the condyle and trochlea...
December 2016: Injury
https://www.readbyqxmd.com/read/28028895/characterization-of-sox9-overexpressing-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-based-engineered-cartilage-both-in-vitro-and-in-vivo
#20
Xiao-Li Li, Jun Zhang, Hua-Nan Luo, Xiaoyan Zhao, A-Ling Zhang, Zheng-Hui Wang
The source of seed cells is a critical factor for tissue engineering . The goal of this study was to evaluate the chondrogenesis of Sox9-overexpressing human umbilical cord mesenchymal stem cells (hUCMSCs) seeded onto bone matrix gelatin (BMG)/fibrin hybrid scaffolds both in vitro and in vivo. hUCMSCs were stably transfected with Sox9-expressing plasmid and grown on the three-dimensional BMG/fibrin hybrid scaffold for 8 weeks. Scanning electron microscopy and histochemistry were performed. The hUCMSC-loaded scaffolds were implanted into the subcutaneous layer of immunocompetent rats and chondrogenesis and host immune responses were monitored for 8 weeks...
December 28, 2016: Journal of Biomedical Materials Research. Part A
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