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Human bone scaffold

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https://www.readbyqxmd.com/read/28927931/polyurethane-foam-scaffold-as-in-vitro-model-for-breast-cancer-bone-metastasis
#1
Valentina Angeloni, Nicola Contessi, Cinzia De Marco, Serena Bertoldi, Maria Cristina Tanzi, Maria Grazia Daidone, Silvia Farè
Breast cancer (BC) represents the most incident cancer case in women (29%), with high mortality rate. Bone metastasis occurs in 20-50% cases and, despite advances in BC research, the interactions between tumor cells and the metastatic microenvironment are still poorly understood. In vitro 3D models gained great interest in cancer research, thanks to the reproducibility, the 3D spatial cues and associated low costs, compared to in vivo and 2D in vitro models. In this study, we investigated the suitability of a poly-ether-urethane (PU) foam as 3D in vitro model to study the interactions between BC tumor-initiating cells and the bone microenvironment...
September 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28927930/biodegradable-and-adjustable-sol-gel-glass-based-hybrid-scaffolds-from-multi-armed-oligomeric-building-blocks
#2
Christian Kascholke, Stephan Hendrikx, Tobias Flath, Dzmitry Kuzmenka, Hans-Martin Dörfler, Dirk Schumann, Mathias Gressenbuch, F Peter Schulze, Michaela Schulz-Siegmund, Michael C Hacker
Biodegradability is a crucial characteristic to improve the clinical potential of sol-gel-derived glass materials. To this end, a set of degradable organic/inorganic class II hybrids from a tetraethoxysilane(TEOS)-derived silica sol and oligovalent cross-linker oligomers containing oligo(D,L-lactide) domains was developed and characterized. A series of 18 oligomers (Mn: 1100-3200 Da) with different degrees of ethoxylation and varying length of oligoester units was established and chemical composition was determined...
September 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28921822/construction-of-human-induced-pluripotent-stem-cell-derived-oriented-bone-matrix-microstructure-by-using-in-vitro-engineered-anisotropic-culture-model
#3
Ryosuke Ozasa, Aira Matsugaki, Yoshihiro Isobe, Taro Saku, Hui-Suk Yun, Takayoshi Nakano
Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold...
September 16, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28919925/in-vitro-evaluation-of-polylactic-acid-pla-manufactured-by-fused-deposition-modeling
#4
Matthias C Wurm, Tobias Möst, Bastian Bergauer, Dominik Rietzel, Friedrich Wilhelm Neukam, Sandra C Cifuentes, Cornelius von Wilmowsky
BACKGROUND: With additive manufacturing (AM) individual and biocompatible implants can be generated by using suitable materials. The aim of this study was to investigate the biological effects of polylactic acid (PLA) manufactured by Fused Deposition Modeling (FDM) on osteoblasts in vitro according to European Norm / International Organization for Standardization 10,993-5. METHOD: Human osteoblasts (hFOB 1.19) were seeded onto PLA samples produced by FDM and investigated for cell viability by fluorescence staining after 24 h...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28919746/in-vitro-and-in-vivo-studies-of-a-novel-bacterial-cellulose-based-acellular-bilayer-nanocomposite-scaffold-for-the-repair-of-osteochondral-defects
#5
Jyoti V Kumbhar, Sachin H Jadhav, Dhananjay S Bodas, Amruta Barhanpurkar-Naik, Mohan R Wani, Kishore M Paknikar, Jyutika M Rajwade
Bacterial cellulose (BC) is a naturally occurring nanofibrous biomaterial which exhibits unique physical properties and is amenable to chemical modifications. To explore whether this versatile material can be used in the treatment of osteochondral defects (OCD), we developed and characterized novel BC-based nanocomposite scaffolds, for example, BC-hydroxyapatite (BC-HA) and BC-glycosaminoglycans (BC-GAG) that mimic bone and cartilage, respectively. In vitro biocompatibility of BC-HA and BC-GAG scaffolds was established using osteosarcoma cells, human articular chondrocytes, and human adipose-derived mesenchymal stem cells...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28917940/natural-and-synthetic-polymers-bioceramics-bioactive-compounds-mediated-cell-signaling-in-bone-tissue-engineering
#6
REVIEW
S Harsha Rao, B Harini, R Pranav Kumar Shadamarshan, K Balagangadharan, N Selvamurugan
Bone is a highly integrative and dynamic tissue of the human body. It is continually remodeled by bone cells such as osteoblasts, osteoclasts. When a fraction of a bone is damaged or deformed, stem cells and bone cells under the influence of several signaling pathways regulate bone regeneration at the particular locale. Effective therapies for bone defects can be met via bone tissue engineering which employs drug delivery systems with biomaterials to enhance cellular functions by acting on signaling pathways such as Wnt, BMP, TGF-β, and Notch...
September 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28917149/a-comparison-study-between-electrospun-polycaprolactone-and-piezoelectric-poly-3-hydroxybutyrate-co-3-hydroxyvalerate-scaffolds-for-bone-tissue-engineering
#7
Svetlana N Gorodzha, Albert R Muslimov, Dina S Syromotina, Alexander S Timin, Nikolai Y Tcvetkov, Kirill V Lepik, Aleksandra V Petrova, Maria A Surmeneva, Dmitry A Gorin, Gleb B Sukhorukov, Roman A Surmenev
In this study, bone scaffolds composed of polycaprolactone (PCL), piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and silicate containing hydroxyapatite (PHBV-SiHA) were successfully fabricated by a conventional electrospinning process. The morphological, chemical, wetting and biological properties of the scaffolds were examined. All fabricated scaffolds are composed of randomly oriented fibres with diameters from 800nm to 12μm...
September 5, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28901689/prevascularization-of-3d-printed-bone-scaffolds-by-bioactive-hydrogels-and-cell-co-culture
#8
Mitchell A Kuss, Shaohua Wu, Ying Wang, Jason B Untrauer, Wenlong Li, Jung Yul Lim, Bin Duan
Vascularization is a fundamental prerequisite for large bone construct development and remains one of the main challenges of bone tissue engineering. Our current study presents the combination of 3D printing technique with a hydrogel-based prevascularization strategy to generate prevascularized bone constructs. Human adipose derived mesenchymal stem cells (ADMSC) and human umbilical vein endothelial cells (HUVEC) were encapsulated within our bioactive hydrogels, and the effects of culture conditions on in vitro vascularization were determined...
September 13, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28900122/projection-stereolithographic-fabrication-of-bmp-2-gene-activated-matrix-for-bone-tissue-engineering
#9
Hang Lin, Ying Tang, Thomas P Lozito, Nicholas Oyster, Robert B Kang, Madalyn R Fritch, Bing Wang, Rocky S Tuan
Currently, sustained in vivo delivery of active bone morphogenetic protein-2 (BMP-2) protein to responsive target cells, such as bone marrow-derived mesenchymal stem cells (BMSCs), remains challenging. Ex vivo gene transfer method, while efficient, requires additional operation for cell culture and therefore, is not compatible with point-of-care treatment. In this study, two lentiviral gene constructs - (1) Lv-BMP/GFP, containing human BMP-2 and green fluorescent protein (GFP) gene (BMP group); or (2) Lv-GFP, containing GFP gene (GFP group) - were incorporated with human BMSCs into a solution of photocrosslinkable gelatin, which was then subjected to visible light-based projection stereolithographic printing to form a scaffold with desired architectures...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28899816/enzyme-crosslinked-gene-activated-matrix-for-the-induction-of-mesenchymal-stem-cells-in-osteochondral-tissue-regeneration
#10
Yi-Hsuan Lee, Hsi-Chin Wu, Chia-Wei Yeh, Chen-Hsiang Kuan, Han-Tsung Liao, Horng-Chaung Hsu, Jui-Che Tsai, Jui-Sheng Sun, Tzu-Wei Wang
The development of osteochondral tissue engineering is an important issue for the treatment of traumatic injury or aging associated joint disease. However, the different compositions and mechanical properties of cartilage and subchondral bone show the complexity of this tissue interface, making it challenging for the design and fabrication of osteochondral graft substitute. In this study, a bilayer scaffold is developed to promote the regeneration of osteochondral tissue within a single integrated construct...
September 9, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28898756/bioreactor-culture-duration-of-engineered-constructs-influences-bone-formation-by-mesenchymal-stem-cells
#11
Debika Mitra, Jacklyn Whitehead, Osamu W Yasui, J Kent Leach
Perfusion culture of mesenchymal stem cells (MSCs) seeded in biomaterial scaffolds provides nutrients for cell survival, enhances extracellular matrix deposition, and increases osteogenic cell differentiation. However, there is no consensus on the appropriate perfusion duration of cellular constructs in vitro to boost their bone forming capacity in vivo. We investigated this phenomenon by culturing human MSCs in macroporous composite scaffolds in a direct perfusion bioreactor and compared their response to scaffolds in continuous dynamic culture conditions on an XYZ shaker...
September 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/28893630/engineered-axially-vascularized-osteogenic-grafts-from-human-adipose-derived-cells-to-treat-avascular-necrosis-of-bone-in-a-rat-model
#12
Tarek Ismail, Rik Osinga, Atanas Todorov, Alexander Haumer, Laurent A Tchang, Christian Epple, Nima Allafi, Nadia Menzi, René D Largo, Alexandre Kaempfen, Ivan Martin, Dirk J Schaefer, Arnaud Scherberich
BACKGROUND: Avascular necrosis of bone (AVN) leads to sclerosis and collapse of bone and joints. The standard of care, vascularized bone grafts, is limited by donor site morbidity and restricted availability. The aim of this study was to generate and test engineered, axially vascularized SVF cells-based bone substitutes in a rat model of AVN. METHODS: SVF cells were isolated from lipoaspirates and cultured onto porous hydroxyapatite scaffolds within a perfusion-based bioreactor system for 5 days...
September 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28892261/fabrication-of-trabecular-bone-templated-tissue-engineered-constructs-by-3d-inkjet-printing
#13
Joseph P Vanderburgh, Shanik J Fernando, Alyssa R Merkel, Julie A Sterling, Scott A Guelcher
3D printing enables the creation of scaffolds with precisely controlled morphometric properties for multiple tissue types, including musculoskeletal tissues such as cartilage and bone. Computed tomography (CT) imaging has been combined with 3D printing to fabricate anatomically scaled patient-specific scaffolds for bone regeneration. However, anatomically scaled scaffolds typically lack sufficient resolution to recapitulate the <100 micrometer-scale trabecular architecture essential for investigating the cellular response to the morphometric properties of bone...
September 11, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28887955/engineered-electrospun-polycaprolactone-pcl-octacalcium-phosphate-ocp-scaffold-for-bone-tissue-engineering
#14
Zohre Heydari, Davod Mohebbi-Kalhori, Mahdi Shafiee Afarani
The main challenge in bone tissue engineering is to find suitable biological substitutes which act as scaffolds. Hence, in this work, a novel scaffold composed of octacalcium phosphate (OCP) particles fabricated by co-precipitation method and polycaprolactone (PCL) using electrospinning technique was introduced. The electrospun scaffolds were characterized by SEM, FTIR, XRD, DSC and TGA analysis. The mechanical properties of the composite scaffolds including maximum tensile stress, strain at break and Young modulus were measured...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28885317/ridge-preservation-after-maxillary-third-molar-extraction-using-30-porosity-plga-ha-%C3%AE-tcp-scaffolds-with-and-without-simvastatin-a-pilot-randomized-controlled-clinical-trial
#15
Miguel Noronha Oliveira, Levy Hermes Rau, Aline Marodin, Márcio Corrêa, Letícia Ruhland Corrêa, Aguedo Aragones, Ricardo de Souza Magini
OBJECTIVE: To evaluate clinically and radiographically, in humans, the healing of maxillary third molars postextraction sockets after application of different ridge preservation techniques 3 months after tooth extraction. MATERIALS AND METHODS: Twenty-six sockets (13 patients) were randomly assigned to 4 treatment modalities: deproteinized bovine bone mineral with 10% collagen (DBBM-C), poly(D,L-lactide-co-glycolide) with hydroxyapatite/β-TCP scaffold (PLGA/HA), PLGA/HA/β-TCP with 2...
September 6, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28884508/the-unique-calcium-chelation-property-of-poly-vinyl-phosphonic-acid-co-acrylic-acid-and-effects-on-osteogenesis-in-vitro
#16
Qiguang Wang, Ian Wimpenny, Rebecca E Dey, Xia Zhong, Peter J Youle, Sandra Downes, David C Watts, Peter M Budd, Judith A Hoyland, Julie E Gough
There is a clear clinical need for a bioactive bone graft substitute. Poly(vinyl phosphonic acid-co-acrylic acid) (PVPA-co-AA) has been identified as a promising candidate for bone regeneration but there is little evidence to show its direct osteogenic effect on progenitor or mature cells. In this study mature osteoblast-like cells (SaOS-2) and human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were cultured with PVPA-co-AA polymers with different VPA:AA ratio and at different concentrations in vitro...
September 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28882369/3d-printed-tcp-based-scaffold-incorporating-vegf-loaded-plga-microspheres-for-craniofacial-tissue-engineering
#17
F Fahimipour, M Rasoulianboroujeni, E Dashtimoghadam, K Khoshroo, M Tahriri, F Bastami, D Lobner, L Tayebi
OBJECTIVE: Vascularization is a critical process during bone regeneration/repair and the lack of tissue vascularization is recognized as a major challenge in applying bone tissue engineering methods for cranial and maxillofacial surgeries. The aim of our study is to fabricate a vascular endothelial growth factor (VEGF)-loaded gelatin/alginate/β-TCP composite scaffold by 3D printing method using a computer-assisted design (CAD) model. METHODS: The paste, composed of (VEGF-loaded PLGA)-containing gelatin/alginate/β-TCP in water, was loaded into standard Nordson cartridges and promptly employed for printing the scaffolds...
September 4, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28880264/knockdown-of-sirt7-enhances-the-osteogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-partly-via-activation-of-the-wnt-%C3%AE-catenin-signaling-pathway
#18
Erman E M Chen, Wei Zhang, Chenyi C Y Ye, Xiang Gao, Liangjun L J Jiang, Tengfei T F Zhao, Zhijun Z J Pan, Deting D T Xue
Sirtuin 7 (SIRT7) is a NAD(+)-dependent deacetylase in the sirtuin family. In a previous study, human bone marrow mesenchymal stem cells (hBMSCs) with reduced SIRT7 activity were developed to evaluate the effect of SIRT7 on osteogenesis. SIRT7 knockdown significantly enhanced osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. Additionally, SIRT7 knockdown upregulated β-catenin. The enhanced osteogenesis due to SIRT7 knockdown was partially rescued by a Wnt/β-catenin inhibitor...
September 7, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28877641/collagen-and-hydroxyapatite-scaffolds-activate-distinct-osteogenesis-signaling-pathways-in-adult-adipose-derived-multipotent-stromal-cells
#19
Wei Duan, Masudul Haque, Michael T Kearney, Mandi Lopez
Osteogenic cell signaling pathway disruption varies among bone diseases. This investigation was designed to identify adipose derived multipotent stromal cell (ASC) and bone graft scaffold combinations for local, targeted restoration of gene expression and extracellular matrix (ECM) deposition. Human ASC osteogenesis on bone graft materials was quantified following culture in stromal (S), osteogenic (O) or osteogenic for 48 hours followed by stromal medium (OS) to test the two-part hypothesis: 1) Identical ASC isolates on distinct bone graft scaffolds demonstrate unique viability, differentiation, ECM production and gene expression in the same culture conditions; 2) Identical ASC-bone graft scaffold combinations have different cell viability, differentiation, ECM production, and gene expression when cultured in S, O or OS medium...
September 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28877412/neuronal-differentiation-of-human-mesenchymal-stem-cells-in-response-to-the-domain-size-of-graphene-substrates
#20
Yoo-Jung Lee, Tae Hoon Seo, Seul-A Lee, Wonhee Jang, Myung Jong Kim, Jung-Suk Sung
Graphene is a non-cytotoxic monolayer platform with unique physical, chemical, and biological properties. It has been demonstrated that graphene substrate may provide a promising biocompatible scaffold for stem cell therapy. Since chemical vapor deposited graphene has a two dimensional poly-crystalline structure, it is important to control the individual domain size to obtain desirable properties for nano-material. However, the biological effects mediated by differences in domain size of graphene have not yet been reported...
September 6, 2017: Journal of Biomedical Materials Research. Part A
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