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Human bone scaffold

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https://www.readbyqxmd.com/read/28636926/enhanced-human-bone-marrow-mesenchymal-stromal-cell-adhesion-on-scaffolds-promotes-cell-survival-and-bone-formation
#1
Miryam Mebarki, Laura Coquelin, Pierre Layrolle, Séverine Battaglia, Marine Tossou, Philippe Hernigou, Hélène Rouard, Nathalie Chevallier
In order to induce an efficient bone formation with human bone marrow mesenchymal stromal cells (hBMSC) associated to a scaffold, it is crucial to determine the key points of the hBMSC action after in vivo transplantation as well as the appropriate features of a scaffold. To this aim we compared the hBMSC behavior when grafted onto two biomaterials allowing different bone potential in vivo. The cancellous devitalized Tutoplast®-processed bone (TPB) and the synthetic hydroxyapatite/β-tricalcium-phosphate (HA/βTCP) which give at 6 weeks 100% and 50% of bone formation respectively...
June 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28635177/engineering-human-bone-grafts-with-new-macroporous-calcium-phosphate-cement-scaffolds
#2
Martina Sladkova, Michael Palmer, Caroline Öhman, Jiayi Cheng, Shoug Al-Ansari, Munerah Saad, Håkan Engqvist, Giuseppe Maria de Peppo
Bone engineering opens the possibility to grow large amounts of tissue products by combining patient-specific cells with compliant biomaterials. Decellularized tissue matrices represent suitable biomaterials but availability, long processing time, excessive cost, and concerns on pathogen transmission have led to the development of biomimetic synthetic alternatives. We recently fabricated calcium phosphate cement (CPC) scaffolds with variable macroporosity using a facile synthesis method with minimal manufacturing steps, and demonstrated long-term biocompatibility in vitro...
June 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28631418/the-effects-of-biodentine-polycaprolactone-3d-scaffold-with-odontogenesis-properties-on-human-dental-pulp-cells
#3
C-C Ho, H-Y Fang, B Wang, T-H Huang, M-Y Shie
AIM: To determine the feasibility of using three-dimensional printed Biodentine/polycaprolactone composite scaffolds for orthopaedic and dental applications. The physicochemical properties and the odontogenic differentiation of human dental pulp cells (hDPCs) were investigated. METHODOLOGY: Biodentine was well-suspended in ethanol and dropped slowly into molten polycaprolactone with vigorous stirring. The Biodentine/polycaprolactone composite scaffolds were then fabricated into controlled macropore sizes and structures using an extrusion-based three-dimensional (3D) printer...
June 20, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28629079/silver-doped-resorbable-tricalcium-phosphate-scaffolds-for-bone-graft-applications
#4
Sean Hoover, Solaiman Tarafder, Amit Bandyopadhyay, Susmita Bose
Bone graft procedures, in particular maxillofacial repair, account for half of the orthopedic procedures done in the US each year. Infection is a major issue in surgery, and should be of primary concern when engineering biomaterials. Silver is of renewed importance today, as it has the ability to potentiate antibiotics against resistant bacterial strains. In order to reduce long term infection risks, it is necessary for the scaffold to maintain a silver ion release for the length of the healing process. In this study, silver doped porous β-tricalcium phosphate (β-TCP) scaffolds were engineered using liquid porogen based method with the goal of meeting these requirements...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629069/evaluation-of-electrospun-biomimetic-substrate-surface-decorated-with-nanohydroxyapatite-precipitation-for-osteoblasts-behavior
#5
Sai Zhang, Guojun Jiang, Molamma P Prabhakaran, Xiaohong Qin, Seeram Ramakrishna
To engineer bone tissue, it is crucial to design scaffolds with micro- and nano-sized architecture imitating approximate hierarchical structure of native bone, and afford desirable biological properties by introducing biocompatible polymers and bioceramics into the scaffolds. Here, a novel scaffold consisting of poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV)/polyaspartic acid (PAA) was fabricated by electrospinning and nano-hydroxyapatite (nHA) was deposited by calcium-phosphate dipping process for bone tissue regeneration...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629066/glucosamine-modified-polyethylene-glycol-hydrogel-mediated-chondrogenic-differentiation-of-human-mesenchymal-stem-cells
#6
Hang Yao, Jingchen Xue, Qunfang Wang, Renjian Xie, Weichang Li, Sa Liu, Jinglei Cai, Dajiang Qin, Dong-An Wang, Li Ren
Glucosamine (GA) is an important cartilage matrix precursor for the glycosaminoglycan biochemical synthesis, and has positive effects on cartilage regeneration, particularly in osteoarthritis therapy. However, it has not been used as a bioactive group in scaffolds for cartilage repair widely. In this study, we synthesized modified polyethylene glycol (PEG) hydrogel with glucosamine and then encapsulated human bone mesenchymal stem cells (hBMSCs) in the hydrogel to induce the differentiation of hBMSCs into chondrocytes in three-dimensional culture...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629047/two-phase-electrospinning-to-incorporate-growth-factors-loaded-chitosan-nanoparticles-into-electrospun-fibrous-scaffolds-for-bioactivity-retention-and-cartilage-regeneration
#7
Chongyang Wang, Wenxiu Hou, Xuran Guo, Juehong Li, Tu Hu, Manle Qiu, Shen Liu, Xiumei Mo, Xudong Liu
Growth factor is an essential ingredient to regulate mesenchymal stem cells (MSCs) chondrogenic differentiation in cartilage tissue engineering. However, non-osteochondral specification, short plasma half time and bioactivity loss restrict growth factor's application. Thus, novel chondrogenic growth factors, specifically target osteochondral lineage cells, that can be sustained release and bioactivity protected to exert functions continually and effectively have attracted increasing researchers' interest. To achieve these goals, chitosan nanoparticles and electrospun fiber scaffolds were used as dual release system to sustain release Nel-like molecule-1 (Nell-1) growth factor and protect bioactivity, then the effect and mechanism of Nell-1 on inducing human bone MSCs (hBMSCs) differentiate toward chondrocytes were investigated...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629021/novel-hipsc-based-tri-culture-for-pre-vascularization-of-calcium-phosphate-scaffold-to-enhance-bone-and-vessel-formation
#8
Chi Zhang, Kevin Hu, Xian Liu, Mark A Reynolds, Chongyun Bao, Ping Wang, Liang Zhao, Hockin H K Xu
Vascularization of tissue-engineered bone is a critical step in maintaining cell viability and advancing cell performance in vivo. In this study, a novel tri-culture system was developed to elicit pre-vascularization of calcium phosphate cement (CPC) scaffold in which human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSMSCs) were seeded together with human umbilical vein endothelial cells (HUVECs) and pericytes. In a two-step methodology design, we first performed osteoinduction of the seeded hiPSMSCs on the CPC scaffold and then incorporated HUVECs and pericytes to the hiPSMSC-colonized CPC scaffold under a favorable culturing condition, with an objective to form a stable and functional capillary-like vascular network that sustained the engineered osseous tissue...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28622604/tissue-engineered-hypertrophic-chondrocyte-grafts-enhanced-long-bone-repair
#9
Jonathan Bernhard, James Ferguson, Bernhard Rieder, Patrick Heimel, Thomas Nau, Stefan Tangl, Heinz Redl, Gordana Vunjak-Novakovic
Bone has innate ability to regenerate following injury. However, large and complex fractures exceed bone's natural repair capacity and result in non-unions, requiring external intervention to facilitate regeneration. One potential treatment solution, tissue-engineered bone grafts, has been dominated by recapitulating intramembranous ossification (bone formation by osteoblasts), although most serious bone injuries heal by endochondral ossification (bone formation by remodeling of hypertrophic cartilaginous anlage)...
September 2017: Biomaterials
https://www.readbyqxmd.com/read/28621746/biocompatibility-analyses-of-al%C3%A2-o%C3%A2-treated-titanium-plates-tested-with-osteocyte-and-fibroblast-cell-lines
#10
Alberto Smargiassi, Jessika Bertacchini, Marta Checchi, Francesco Cavani, Marzia Ferretti, Carla Palumbo
Osseointegration of a titanium implant is still an issue in dental/orthopedic implants durable over time. The good integration of these implants is mainly due to their surface and topography. We obtained an innovative titanium surface by shooting different-in-size particles of Al₂O₃ against the titanium scaffolds which seems to be ideal for bone integration. To corroborate that, we used two different cell lines: MLO-Y4 (murine osteocytes) and 293 (human fibroblasts) and tested the titanium scaffolds untreated and treated (i...
June 16, 2017: Biomedicines
https://www.readbyqxmd.com/read/28617737/3d-cone-beam-computed-tomography-volumetric-outcomes-of-rhbmp-2-demineralized-bone-matrix-vs-iliac-crest-bone-graft-for-alveolar-cleft-reconstruction
#11
Fan Liang, Stephen Yen, Thomas Imahiyerobo, Luke Sanborn, Leia Yen, Daniel Yen, Sheila Nazarian, Breanna Jedrzejewski, Mark Urata, Jeffrey Hammoudeh
BACKGROUND: Recent studies indicate that recombinant human bone morphogenetic protein (rhBMP-2) in a demineralized bone matrix scaffold (MS) is a comparable alternative to iliac bone autograft in the setting of secondary alveolar cleft repair. Post-reconstruction occlusal radiographs demonstrate improved bone stock when rhBMP-2/MS is used, but lack the capacity to evaluate bone growth in three dimensions. This study uses cone beam computed tomography (CBCT) to provide the first clinical evaluation of volumetric and density comparisons between these two treatment modalities...
June 14, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28611002/chondrogenesis-of-human-bone-marrow-mesenchymal-stem-cells-in-3-dimensional-photocrosslinked-hydrogel-constructs-effect-of-cell-seeding-density-and-material-stiffness
#12
Aaron X Sun, Hang Lin, Madalyn R Fritch, He Shen, Pete G Alexander, Michael DeHart, Rocky S Tuan
Three-dimensional hydrogel constructs incorporated with live stem cells that support chondrogenic differentiation and maintenance offer a promising regenerative route towards addressing the limited self-repair capabilities of articular cartilage. In particular, hydrogel scaffolds that augment chondrogenesis and recapitulate the native physical properties of cartilage, such as compressive strength, can potentially be applied in point-of-care procedures. We report here the synthesis of two new materials, [poly-l-lactic acid/polyethylene glycol/poly-l-lactic acid] (PLLA-PEG 1000) and [poly-d,l-lactic acid/polyethylene glycol/poly-d,l-lactic acid] (PDLLA-PEG 1000), that are biodegradable, biocompatible (>80% viability post fabrication), and possess high, physiologically relevant mechanical strength (∼1500 to 1800kPa)...
June 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28607223/graphene-oxide-improves-the-biocompatibility-of-collagen-membranes-in-an-in-vitro-model-of-human-primary-gingival-fibroblasts
#13
Patrizia De Marco, Susi Zara, Marianna De Colli, Milena Radunovic, Vladimir Lazovic, Valeria Ettorre, Antonello Di Crescenzo, Adriano Piattelli, Amelia Cataldi, Antonella Fontana
Commercial collagen membranes are used in oral surgical procedures as scaffolds for bone deposition in guided bone regeneration. Here, we have enriched them with graphene oxide (GO) via a simple non-covalent functionalization and exploiting the capacity of oxygenated carbon functional moieties of GO to interact through hydrogen bonding with collagene. In the present paper, the GO-coated membranes have been characterized in terms of stability, nano-roughness, biocompatibility and induction of inflammatory response towards HGFs cells...
June 13, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28605908/chondroitin-sulfate-based-biomineralizing-surface-hydrogels-for-bone-tissue-engineering
#14
Hwan D Kim, Eunjee A Lee, Young-Hyeon An, Seunghyun L Kim, Seunghun S Lee, Seung Jung Yu, Hae Lin Jang, Ki Tae Nam, Sung Gap Im, Nathaniel S Hwang
Chondroitin sulfate (CS) is the major component of glycosaminoglycan in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated them as biomineralizing three-dimensional scaffolds for charged ion binding and depositions. Due to its negative charge from the sulfate group, CS exhibited an osteogenically favorable microenvironment by binding charged ions such as calcium and phosphate. Particularly, ion binding and distribution within negatively charged hydrogel was dependent on CS concentration...
June 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28589918/cryogenic-3d-printing-for-producing-hierarchical-porous-and-rhbmp-2-loaded-ca-p-plla-nanocomposite-scaffolds-for-bone-tissue-engineering
#15
Chong Wang, Qilong Zhao, Min Wang
The performance of bone tissue engineering scaffolds can be assessed through cell responses to scaffolds, including cell attachment, infiltration, morphogenesis, proliferation, differentiation, etc, which are determined or heavily influenced by the composition, structure, mechanical properties, and biological properties (e.g. osteoconductivity and osteoinductivity) of scaffolds. Although some promising 3D printing techniques such as fused deposition modeling and selective laser sintering could be employed to produce biodegradable bone tissue engineering scaffolds with customized shapes and tailored interconnected pores, effective methods for fabricating scaffolds with well-designed hierarchical porous structure (both interconnected macropores and surface micropores) and tunable osteoconductivity/osteoinductivity still need to be developed...
June 7, 2017: Biofabrication
https://www.readbyqxmd.com/read/28589787/conditioned-medium-from-the-three-dimensional-culture-of-human-umbilical-cord-perivascular-cells-accelerate-the-migration-and-proliferation-of-human-keratinocyte-and-fibroblast
#16
Min Ho Kim, Wen Hao Wu, Jee Hyun Choi, Ji Hyun Kim, Seok-Ho Hong, Jin Hyun Jun, Yong Ko, Jong Hun Lee
Previous studies have reported that the conditioned medium (CM) of bone marrow-mesenchymal stem cells (BM-MSCs) stimulate the migration and proliferation of cell types involved in the wound healing process. However, these studies only show MSC-CM effects that were obtained using a two-dimensional (2D) culture. Recently, a three-dimensional (3D) culture has been considered to be a more physiologically appropriate system than the 2D culture. In addition, it has been shown that the procurement of BM-MSC is invasive, and other sources of MSC are thus being explored...
June 18, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28586346/3d-bioprinting-of-methacrylated-hyaluronic-acid-meha-hydrogel-with-intrinsic-osteogenicity
#17
Michelle T Poldervaart, Birgit Goversen, Mylene de Ruijter, Anna Abbadessa, Ferry P W Melchels, F Cumhur Öner, Wouter J A Dhert, Tina Vermonden, Jacqueline Alblas
In bone regenerative medicine there is a need for suitable bone substitutes. Hydrogels have excellent biocompatible and biodegradable characteristics, but their visco-elastic properties limit their applicability, especially with respect to 3D bioprinting. In this study, we modified the naturally occurring extracellular matrix glycosaminoglycan hyaluronic acid (HA), in order to yield photo-crosslinkable hydrogels with increased mechanical stiffness and long-term stability, and with minimal decrease in cytocompatibility...
2017: PloS One
https://www.readbyqxmd.com/read/28583182/tissue-specific-bioactivity-of-soluble-tendon-derived-and-cartilage-derived-extracellular-matrices-on-adult-mesenchymal-stem-cells
#18
Benjamin B Rothrauff, Guang Yang, Rocky S Tuan
BACKGROUND: Biological scaffolds composed of tissue-derived extracellular matrix (ECM) can promote homologous (i.e., tissue-specific) cell differentiation through preservation of biophysical and biochemical motifs found in native tissues. Solubilized ECMs derived from decellularized tendon and cartilage have recently been promoted as tissue-specific biomaterials, but whether tissue-specific bioactivity is preserved following solubilization is unknown. This study explored the tissue-specific bioactivity of soluble decellularized tendon and cartilage ECMs on human bone marrow-derived mesenchymal stem cells (MSCs) presented across different culture microenvironments, including two-dimensional (2D) tissue culture plastic, aligned electrospun nanofibers, cell pellets, and cell-seeded photocrosslinkable hydrogels...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28576047/optimally-designed-collagen-polycaprolactone-biocomposites-supplemented-with-controlled-release-of-ha-tcp-rhbmp-2-and-ha-tcp-prp-for-hard-tissue-regeneration
#19
WonJin Kim, Chul Ho Jang, GeunHyung Kim
Collagen has been widely used as a very promising material to regenerate various tissues. It is a chief component of the extracellular matrix, and encourages various biological effects conducive to tissue regeneration. However, poor mechanical stability, low processability, and high level of water absorption can lead to impaired control of growth factor release and have impeded the use of collagen as a functional biomedical scaffold. Here, to overcome the shortcomings of collagen scaffolds, we have additively manufactured collagen/polycaprolactone (PCL) biocomposites supplemented with a bioceramic (hydroxyapatite (HA)/β-tricalcium-phosphate (TCP)) and two growth factors (recombinant human bone morphogenetic protein-2 [rhBMP-2] and platelet-rich plasma [PRP])...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28576015/porous-nb-ti-ta-alloy-scaffolds-for-bone-tissue-engineering-fabrication-mechanical-properties-and-in-vitro-vivo-biocompatibility
#20
Jue Liu, Jianming Ruan, Lin Chang, Hailin Yang, Wei Ruan
Porous Nb-Ti-Ta (at.%) alloys with the pore size of 100-600μm and the porosity of 50%-80% were fabricated by the combination of the sponge impregnation technique and sintering method. The results revealed that the pores were well connected with three-dimensional (3D) network structure, which showed morphological similarity to the anisotropic porous structure of human bones. The results also showed that the alloys could provide the compressive Young's modulus of 0.11±0.01GPa to 2.08±0.09GPa and the strength of 17...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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