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Human bone scaffold

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https://www.readbyqxmd.com/read/29786553/from-skeletal-development-to-the-creation-of-pluripotent-stem-cell-derived-bone-forming-progenitors
#1
REVIEW
Wai Long Tam, Frank P Luyten, Scott J Roberts
Bone has many functions. It is responsible for protecting the underlying soft organs, it allows locomotion, houses the bone marrow and stores minerals such as calcium and phosphate. Upon damage, bone tissue can efficiently repair itself. However, healing is hampered if the defect exceeds a critical size and/or is in compromised conditions. The isolation or generation of bone-forming progenitors has applicability to skeletal repair and may be used in tissue engineering approaches. Traditionally, bone engineering uses osteochondrogenic stem cells, which are combined with scaffold materials and growth factors...
July 5, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29779220/human-intrabony-defect-regeneration-with-micro-grafts-containing-dental-pulp-stem-cells-a-randomized-controlled-clinical-trial
#2
Francesco Ferrarotti, Federica Romano, Mara Noemi Gamba, Andrea Quirico, Marta Giraudi, Martina Audagna, Mario Aimetti
AIM: The goal of this study was to evaluate if dental pulp stem cells (DPSCs) delivered into intrabony defects in a collagen scaffold would enhance the clinical and radiographic parameters of periodontal regeneration. MATERIALS AND METHODS: In this randomized controlled trial, 29 chronic periodontitis patients presenting one deep intrabony defect and requiring extraction of one vital tooth were consecutively enrolled. Defects were randomly assigned to test or control treatments which both consisted of the use of minimally invasive surgical technique...
May 19, 2018: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29768878/nano-nets-covered-composite-nanofibers-with-enhanced-biocompatibility-and-mechanical-properties-for-bone-tissue-engineering
#3
Arjun Prasad Tiwari, Mahesh Kumar Joshi, Chan Hee Park, Cheol Sang Kim
Enhancing the biocompatibility profiles including cell attachment, growth, and viability and mechanical properties of designed synthetic scaffolds have an essential role in tissue engineering applications. Polymer blending is one of the most effective methods for providing new anticipated biomaterials for tissue scaffolds. Here, the blend solution of the different mass weight ratio of polycaprolactone (PCL) to human serum albumin (HSA) was subjected to fabricate nanocomposite spider-web-like membranes using electrospinning process...
January 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29767463/mechanical-and-biological-properties-of-a-biodegradable-mg-zn-ca-porous-alloy
#4
Yong-Qiang Zhang, Yang Li, Huan Liu, Jing Bai, Ni-Rong Bao, Yue Zhang, Peng He, Jian-Ning Zhao, Li Tao, Feng Xue, Guang-Xin Zhou, Gen-Tao Fan
OBJECTIVES: As promising alternative to current metallic biomaterials, the porous Mg scaffold with a 3-D open-pore framework has drawn much attention in recent years due to its suitable biodegradation, biocompatibility, and mechanical properties for human bones. This experiment's aim is to study the mechanical properties, biosafety, and osteogenesis of porous Mg-Zn alloy. METHODS: A porous Mg-2Zn-0.3Ca (wt%) alloy was successfully prepared by infiltration casting, and the size of NaCl particles was detected by a laser particle size analyzer...
May 16, 2018: Orthopaedic Surgery
https://www.readbyqxmd.com/read/29765415/human-recombinant-peptide-sponge-enables-novel-less-invasive-cell-therapy-for-ischemic-stroke
#5
Michiyuki Miyamoto, Kentaro Nakamura, Hideo Shichinohe, Tomohiro Yamauchi, Masaki Ito, Hisayasu Saito, Masahito Kawabori, Toshiya Osanai, Tasuku Sasaki, Kiyohiro Houkin, Satoshi Kuroda
Bone marrow stromal cell (BMSC) transplantation has the therapeutic potential for ischemic stroke. However, it is unclear which delivery routes would yield both safety and maximal therapeutic benefits. We assessed whether a novel recombinant peptide (RCP) sponge, that resembles human collagen, could act as a less invasive and beneficial scaffold in cell therapy for ischemic stroke. BMSCs from green fluorescent protein-transgenic rats were cultured and Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAo)...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29761787/osteoinducing-scaffolds-with-multi-layered-biointerface
#6
Ekaterina A Grebenik, Varvara Grinchenko, Semyon Nikolaevich Churbanov, Nikita Minaev, Boris Shavkuta, Pavel Alexandrovich Melnikov, Denis Butnaru, Yury Rochev, Victor Nikolayevich Bagratashvili, Peter S Timashev
This study was aimed to design and characterize hybrid tissue-engineered constructs composed of osteoinducing polylactide-based scaffolds with multi-layered cellular biointerface for bone tissue reconstruction. Three-dimensional scaffolds with improved hydrophilic and osteoinducing properties were produced using the surface-selective laser sintering (SSLS) method. The designed scaffold pattern had dimensions of 8×8×2.5 mm and ladder-like pores (~ 700 μm in width). Hyaluronic acid-coated polylactide microparticles (~ 100 μm in diameter) were used as building blocks and water was used as the photosensitizer for SSLS followed by photocross-linking with Irgacure 2959 photoinitiator...
May 15, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29756533/genetic-engineering-of-mesenchymal-stem-cells-for-differential-matrix-deposition-on-3dwoven-scaffold
#7
Nguyen Phuong Thao Huynh, Jonathan Matthew Brunger, Catherine Claire Gloss, Franklin T Moutos, Charles A Gersbach, Farshid Guilak
Tissue engineering approaches for the repair of osteochondral defects using biomaterial scaffolds and stem cells have remained challenging due to the inherent complexities of inducing cartilage-like matrix and bone-like matrix within the same local environment. Members of the transforming growth factor β (TGFβ) family have been extensively utilized in the engineering of skeletal tissues, but have distinct effects on chondrogenic and osteogenic differentiation of progenitor cells. The goal of this study was to develop a method to direct human bone marrow derived mesenchymal stem cells (MSCs) to deposit either mineralized matrix or a cartilaginous matrix rich in glycosaminoglycan and type II collagen within the same biochemical environment...
May 14, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29756272/harnessing-human-decellularized-blood-vessel-matrices-and-cellular-construct-implants-to-promote-bone-healing-in-an-ex-vivo-organotypic-bone-defect-model
#8
Stefanie Inglis, Karl Heinrich Schneider, Janos M Kanczler, Heinz Redl, Richard O C Oreffo
Decellularized matrices offer a beneficial substitute for biomimetic scaffolds in tissue engineering. The current study examines the potential of decellularized placental vessel sleeves (PVS) as a periosteal protective sleeve to enhance bone regeneration in embryonic day 18 chick femurs contained within the PVS and cultured organotypically over a 10 day period. The femurs are inserted into decellularized biocompatibility-tested PVS and maintained in an organotypic culture for a period of 10 days. In femurs containing decellularized PVS, a significant increase in bone volume (p < 0...
May 14, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29752122/strontium-modified-chitosan-montmorillonite-composites-as-bone-tissue-engineering-scaffold
#9
Aysel Koç Demir, Ayşe Eser Elçin, Yaşar Murat Elçin
The objective of this study is to develop chitosan/montmorillonite (C/MMT) composite scaffolds based on improved properties for bone tissue engineering applications. With the freeze-drying technique, strontium (Sr2+ ) modified C/MMT composite scaffold with an interconnected porous structure was produced. X-ray diffraction, fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy (SEM) were employed to investigate the structural properties, surface morphology and porosity of the composite scaffold...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29752081/effect-of-polyhydroxybutyrate-chitosan-bioglass-nanofiber-scaffold-on-proliferation-and-differentiation-of-stem-cells-from-human-exfoliated-deciduous-teeth-into-odontoblast-like-cells
#10
Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Batool Hashemibeni, Abbas Ali Khademi
Scaffolds and their characteristics play a central role in tissue engineering. The purpose of this study was to determine the effects of Polyhydroxybutyrate (PHB)/Chitosan/nano-bioglass (nBG) nanofiber scaffold made using the electrospinning method, on the proliferation and differentiation of stem cells obtained from human exfoliated deciduous teeth into odontoblast-like cells. In this experimental study, the pulps of the molten deciduous teeth were isolated, thereafter, the stem cells from human exfoliated deciduous teeth (SHED) were extracted and then the 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability percentage...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29749273/enhanced-osteogenic-activity-with-boron-doped-nanohydroxyapatite-loaded-poly-butylene-adipate-co-terephthalate-fibrous-3d-matrix
#11
Aysu Arslan, Soner Çakmak, Menemşe Gümüşderelioğlu
In this study, three dimensional (3D) poly(butylene adipate-co-terephthalate) (PBAT) fibrous scaffolds with more than 90% porosity were fabricated via wet electrospinning method. Amorphous hydroxyapatite (HAp) and boron (B) doped hydroxyapatite (B-HAp) nanoparticles were produced by microwave-assisted biomimetic precipitation and encapsulated into PBAT fibres with the ratio of 5% (w/w) in order to enhance osteogenic activity of the scaffolds. Cell culture studies were carried out with human bone marrow derived stem cells (hBMSCs) and they showed that alkaline phosphatase (ALP) activity and the amounts of collagen and calcium were higher on B containing PBAT (B-HAp-PBAT) scaffolds during the 28-day culture period than that of the PBAT scaffolds...
May 11, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29744424/-in-situ-solvothermal-processing-of-polycaprolactone-hydroxyapatite-nanocomposites-with-enhanced-mechanical-and-biological-performance-for-bone-tissue-engineering
#12
Saeed Moeini, Mohammad Reza Mohammadi, Abdolreza Simchi
The interest in biodegradable polymer-matrix nanocomposites with bone regeneration potential has been increasing in recent years. In the present work, a solvothermal process is introduced to prepare hydroxyapatite (HA) nanorod-reinforced polycaprolactone in-situ . A non-aqueous polymer solution containing calcium and phosphorous precursors is prepared and processed in a closed autoclave at different temperatures in the range of 60-150 °C. Hydroxyapatite nanorods with varying aspect ratios are formed depending on the processing temperature...
September 2017: Bioactive Materials
https://www.readbyqxmd.com/read/29744421/smart-biomaterials-surfaces-functionalized-with-proteolytically-stable-osteoblast-adhesive-peptides
#13
Annj Zamuner, Paola Brun, Michele Scorzeto, Giuseppe Sica, Ignazio Castagliuolo, Monica Dettin
Engineered scaffolds for bone tissue regeneration are designed to promote cell adhesion, growth, proliferation and differentiation. Recently, covalent and selective functionalization of glass and titanium surfaces with an adhesive peptide (HVP) mapped on [351-359] sequence of human Vitronectin allowed to selectively increase osteoblast attachment and adhesion strength in in vitro assays, and to promote osseointegration in in vivo studies. For the first time to our knowledge, in this study we investigated the resistance of adhesion sequences to proteolytic digestion: HVP was completely cleaved after 5 h...
September 2017: Bioactive Materials
https://www.readbyqxmd.com/read/29744414/human-adipose-derived-stem-cells-are-superior-to-human-osteoblasts-hob-in-bone-tissue-engineering-on-a-collagen-fibroin-elr-blend
#14
Esen Sayin, Rosti Hama Rashid, José Carlos Rodríguez-Cabello, Ahmed Elsheikh, Erkan Türker Baran, Vasif Hasirci
The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen - silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films...
June 2017: Bioactive Materials
https://www.readbyqxmd.com/read/29743648/a-tpms-based-method-for-modeling-porous-scaffolds-for-bionic-bone-tissue-engineering
#15
Jianping Shi, Liya Zhu, Lan Li, Zongan Li, Jiquan Yang, Xingsong Wang
In the field of bone defect repair, gradient porous scaffolds have received increased attention because they provide a better environment for promoting tissue regeneration. In this study, we propose an effective method to generate bionic porous scaffolds based on the TPMS (triply periodic minimal surface) and SF (sigmoid function) methods. First, cortical bone morphological features (e.g., pore size and distribution) were determined for several regions of a rabbit femoral bone by analyzing CT-scans. A finite element method was used to evaluate the mechanical properties of the bone at these respective areas...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29743489/magnetic-mesoporous-calcium-sillicate-chitosan-porous-scaffolds-for-enhanced-bone-regeneration-and-photothermal-chemotherapy-of-osteosarcoma
#16
Fan Yang, Jiawei Lu, Qinfei Ke, Xiaoyuan Peng, Yaping Guo, Xuetao Xie
The development of multifunctional biomaterials to repair bone defects after neoplasm removal and inhibit tumor recurrence remained huge clinical challenges. Here, we demonstrate a kind of innovative and multifunctional magnetic mesoporous calcium sillicate/chitosan (MCSC) porous scaffolds, made of M-type ferrite particles (SrFe12 O19 ), mesoporous calcium silicate (CaSiO3 ) and chitosan (CS), which exert robust anti-tumor and bone regeneration properties. The mesopores in the CaSiO3 microspheres contributed to the drug delivery property, and the SrFe12 O19 particles improved photothermal therapy (PTT) conversion efficacy...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29732529/osteostimulation-scaffolds-of-stem-cells-bmp-7-derived-peptide-decorated-alginate-porous-scaffolds-promote-the-aggregation-and-osteo-differentiation-of-human-mesenchymal-stem-cells
#17
Yue Yang, Zuyuan Luo, Ying Zhao
The scaffolds for stem cell-based bone tissue engineering should hold the ability to guide stem cells osteo-differentiating. Otherwise, stem cells will differentiate into unwanted cell types or will form tumors in vivo. Alginate, a natural polysaccharide with great biocompatibility, was widely used in biomedical applications. However, the limited bioactivity and poor osteogenesis capability of pristine alginate hampered its further application in tissue engineering. In this work, a bone forming peptide-1 (BFP-1), derived from bone morphogenetic protein-7, was grafted to alginate polymer chains to prepare peptide-decorated alginate porous scaffolds (pep-APS) for promoting osteo-differentiation of human mesenchymal stem cells (hMSCs)...
May 6, 2018: Biopolymers
https://www.readbyqxmd.com/read/29730855/chitosan-gelatin-scaffolds-support-bone-regeneration
#18
Anthie Georgopoulou, Fotios Papadogiannis, Aristea Batsali, John Marakis, Kalliopi Alpantaki, Aristides G Eliopoulos, Charalampos Pontikoglou, Maria Chatzinikolaidou
Chitosan/Gelatin (CS:Gel) scaffolds were fabricated by chemical crosslinking with glutaraldehyde or genipin by freeze drying. Both crosslinked CS:Gel scaffold types with a mass ratio of 40:60% form a gel-like structure with interconnected pores. Dynamic rheological measurements provided similar values for the storage modulus and the loss modulus of the CS:Gel scaffolds when crosslinked with the same concentration of glutaraldehyde vs. genipin. Compared to genipin, the glutaraldehyde-crosslinked scaffolds supported strong adhesion and infiltration of pre-osteoblasts within the pores as well as survival and proliferation of both MC3T3-E1 pre-osteoblastic cells after 7 days in culture, and human bone marrow mesenchymal stem cells (BM-MSCs) after 14 days in culture...
May 5, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29728785/enhanced-in-vivo-bone-and-blood-vessel-formation-by-iron-oxide-and-silica-doped-3d-printed-tricalcium-phosphate-scaffolds
#19
Susmita Bose, Dishary Banerjee, Samuel Robertson, Sahar Vahabzadeh
Calcium phosphate (CaP) ceramics show significant promise towards bone graft applications because of the compositional similarity to inorganic materials of bone. With 3D printing, it is possible to create ceramic implants that closely mimic the geometry of human bone and can be custom-designed for unusual injuries or anatomical sites. The objective of the study was to optimize the 3D-printing parameters for the fabrication of scaffolds, with complex geometry, made from synthesized tricalcium phosphate (TCP) powder...
May 4, 2018: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29725268/mesoporous-bioactive-glass-coated-3d-printed-borosilicate-bioactive-glass-scaffolds-for-improving-repair-of-bone-defects
#20
Xin Qi, Hui Wang, Yadong Zhang, Libin Pang, Wei Xiao, Weitao Jia, Shichang Zhao, Deping Wang, Wenhai Huang, Qiugen Wang
Background: In the field of tissue engineering, there is currently increasing interest in new biomedical materials with high osteogenic ability and comparable mechanical function to repair bone defects. Three-dimensional (3-D) bioactive borosilicate glass (BG) scaffolds exhibit uniform interconnected macro-pores, high porosity and high compressive strength. In this study, we fabricated 3-D BG scaffolds by the 3D printing technique, then coated the surface of the 3-D BG scaffolds with mesoporous bioactive glass (MBG) (BG-MBG scaffold)...
2018: International Journal of Biological Sciences
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