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Human bone scaffold

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https://www.readbyqxmd.com/read/27912139/repair-of-calvarial-bone-defects-in-mice-using-electrospun-polystyrene-scaffolds-combined-with-%C3%AE-tcp-or-gold-nanoparticles
#1
Lisa Terranova, Diana Maria Dragusin, Romain Mallet, Eugeniu Vasile, Izabela-Cristina Stancu, Catherine Behets, Daniel Chappard
Non-biodegradable porous polystyrene (PS) scaffolds, composed of microfibers, have been prepared by electrospinning for the reconstruction of large bone defects. PS microfibers were prepared by incorporating β-TCP grains inside the polymer or grafting gold nanoparticles surface functionalized with mercaptosuccinic acid. Cytocompatibility of the three types of scaffolds (PS, β-TCP-PS and Au-PS) was studied by seeding human mesenchymal stem cells. Biocompatibility was evaluated by implanting β-TCP-PS and Au-PS scaffolds into a critical size (4mm) calvarial defect in mice...
November 8, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27907202/a-reproducible-method-for-isolation-and-in-vitro-culture-of-functional-human-lymphoid-stromal-cells-from-tonsils
#2
Yotam E Bar-Ephraim, Tanja Konijn, Mehmet Gönültas, Reina E Mebius, Rogier M Reijmers
The stromal compartment of secondary lymphoid organs is classicaly known for providing a mechanical scaffold for the complex interactions between hematopoietic cells during immune activation as well as for providing a niche which is favorable for survival of lymphocytes. In recent years, it became increasingly clear that these cells also play an active role during such a response. Currently, knowledge of the interactions between human lymphoid stroma and hematopoietic cells is still lacking and most insight is based on murine systems...
2016: PloS One
https://www.readbyqxmd.com/read/27901555/osteoblast-seeded-bioglass-gelatin-nanocomposite-a-promising-bone-substitute-in-critical-size-calvarial-defect-repair-in-rat
#3
Behrooz Johari, Mehdi Kadivar, Shirin Lak, Mazaher Gholipourmalekabadi, Aleksandra M Urbanska, Masoud Mozafari, Maryam Ahmadzadehzarajabad, Asaad Azarnezhad, Samane Afshari, Jamil Zargan, Saeid Kargozar
INTRODUCTION: Amid the plethora of methods to repair critical bone defects, there is no one perfect approach. In this study, we sought to evaluate a potent 3-dimensional (3D) bioactive SiO2-CaO-P2O5 glasses (bioglass)/gelatin (gel) scaffold for its biocompatibility by seeding cells as well as for its regenerative properties by animal implantation. METHODS: Osteoblast cells were seeded onto nanocomposite scaffolds to investigate the process of critical-size calvarial defect via new bone formation...
November 29, 2016: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/27901202/in-vivo-performance-of-different-scaffolds-for-dental-pulp-stem-cells-induced-for-odontogenic-differentiation
#4
Cigdem Atalayin, Huseyin Tezel, Taner Dagci, Nefise Ulku Karabay Yavasoglu, Gulperi Oktem, Timur Kose
This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. Mesenchymal stem cell medium was used in the control groups. DPSC were transplanted onto the backs of mice via three scaffolds: copolymer of L-lactide and DL-lactide (PLDL), copolymer of DL-lactide (PDL) and hydroxyapatite tricalcium phosphate (HA/TCP)...
November 28, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27895039/region-specific-effect-of-the-decellularized-meniscus-extracellular-matrix-on-mesenchymal-stem-cell-based-meniscus-tissue-engineering
#5
Kazunori Shimomura, Benjamin B Rothrauff, Rocky S Tuan
BACKGROUND: The meniscus is the most commonly injured knee structure, and surgical repair is often ineffective. Tissue engineering-based repair or regeneration may provide a needed solution. Decellularized, tissue-derived extracellular matrices (ECMs) have received attention for their potential use as tissue-engineered scaffolds. In considering meniscus-derived ECMs (mECMs) for meniscus tissue engineering, it is noteworthy that the inner and outer regions of the meniscus have different structural and biochemical features, potentially directing the differentiation of cells toward region-specific phenotypes...
November 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27893514/improved-bone-regeneration-with-multiporous-plga-scaffold-and-bmp-2-transduced-human-adipose-derived-stem-cells-by-cell-permeable-peptide
#6
Suhyun Park, Hyun-A Heo, Kwang-Bae Lee, Han-Goo Kim, Sung-Woon Pyo
OBJECTIVE: Currently, much work has focused on the engineering of bone using adipose-derived stem cells (ADSCs), which differentiate into osteogenic cells. This study was conducted to assess the bone-regenerating capacity of ADSCs with genetic modification. MATERIALS AND METHODS: ADSCs were cultured and transduced with recombinant adenovirus-expressing bone morphogenetic protein-2 (rAd/BMP-2). Two 5-mm full-thickness bone defects were created on the parietal bones of 24 rats...
November 23, 2016: Implant Dentistry
https://www.readbyqxmd.com/read/27886552/three-dimensional-electrospun-pcl-pla-blend-nanofibrous-scaffolds-with-significantly-improved-stem-cells-osteogenic-differentiation-and-cranial-bone-formation
#7
Qingqing Yao, Jaqueline G L Cosme, Tao Xu, Jacob M Miszuk, Paulo H S Picciani, Hao Fong, Hongli Sun
Nanofibrous scaffolds that are morphologically/structurally similar to natural ECM are highly interested for tissue engineering; however, the electrospinning technique has the difficulty in directly producing clinically relevant 3D nanofibrous scaffolds with desired structural properties. To address this challenge, we have developed an innovative technique of thermally induced nanofiber self-agglomeration (TISA) recently. The aim of this work was to prepare (via the TISA technique) and evaluate 3D electrospun PCL/PLA blend (mass ratio: 4/1) nanofibrous scaffolds having high porosity of ∼95...
November 15, 2016: Biomaterials
https://www.readbyqxmd.com/read/27881642/clinoptilolite-pcl-peg-pcl-composite-scaffolds-for-bone-tissue-engineering-applications
#8
Engin Pazarçeviren, Özge Erdemli, Dilek Keskin, Ayşen Tezcaner
The aim of this study was to prepare and characterize highly porous clinoptilolite/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) composite scaffolds. Scaffolds with different clinoptilolite contents (10% and 20%) were fabricated with reproducible solvent-free powder compression/particulate leaching technique. The scaffolds had interconnective porosity in the range of 55-76%. Clinoptilolite/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) scaffolds showed negligible degradation within eight weeks and displayed less water uptake and higher bioactivity than poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) scaffolds...
November 23, 2016: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/27872012/biomimetic-collagen-elastin-meshes-for-ventral-hernia-repair-in-a-rat-model
#9
Silvia Minardi, Francesca Taraballi, Xin Wang, Fernando J Cabrera, Jeffrey L Van Eps, Andrew B Robbins, Monica Sandri, Michael R Moreno, Bradley K Weiner, Ennio Tasciotti
: Ventral hernia repair remains a major clinical need. Herein, we formulated a type I collagen/elastin crosslinked blend (CollE) for the fabrication of biomimetic meshes for ventral hernia repair. To evaluate the effect of architecture on the performance of the implants, CollE was formulated both as flat sheets (CollE Sheets) and porous scaffolds (CollE Scaffolds). The morphology, hydrophylicity and in vitro degradation were assessed by SEM, water contact angle and differential scanning calorimetry, respectively...
November 18, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27871909/histone-deacetylase-inhibition-enhances-in-vivo-bone-regeneration-induced-by-human-periodontal-ligament-cells
#10
Nam Cong-Nhat Huynh, Vincent Everts, Akira Nifuji, Prasit Pavasant, Ruchanee Salingcarnboriboon Ampornaramveth
: Periodontal ligament cells have the potential to differentiate into bone forming osteoblasts and thus represent a good cellular candidate for bone regeneration. This study aimed to investigate the effect of inhibition of histone deacetylases, using the inhibitor Trichostatin A (TSA), on bone regeneration by human periodontal ligament cells (hPDLCs) in a mouse calvaria bone defect. METHODS: RUNX2 protein and its acetylation was analyzed by immunoprecipitation and western blotting...
November 18, 2016: Bone
https://www.readbyqxmd.com/read/27865962/pharmacologically-active-microcarriers-delivering-bdnf-within-a-hydrogel-novel-strategy-for-human-bone-marrow-derived-stem-cells-neural-neuronal-differentiation-guidance-and-therapeutic-secretome-enhancement
#11
Saikrishna Kandalam, Laurence Sindji, Gaëtan J-R Delcroix, Fabien Violet, Xavier Garric, Emilie M André, Paul C Schiller, Marie-Claire Venier-Julienne, Anne des Rieux, Jérôme Guicheux, Claudia N Montero-Menei
: Stem cells combined with biodegradable injectable scaffolds releasing growth factors hold great promises in regenerative medicine, particularly in the treatment of neurological disorders. We here integrated human marrow-isolated adult multilineage-inducible (MIAMI) stem cells and pharmacologically active microcarriers (PAMs) into an injectable non-toxic silanized-hydroxypropyl methylcellulose (Si-HPMC) hydrogel. The goal is to obtain an injectable non-toxic cell and growth factor delivery device...
November 16, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27862947/osteogenic-potential-of-stem-cells-seeded-bioactive-nanocomposite-scaffolds-a-comparative-study-between-human-mesenchymal-stem-cells-derived-from-bone-umbilical-cord-wharton-s-jelly-and-adipose-tissue
#12
Saeid Kargozar, Masoud Mozafari, Seyed Jafar Hashemian, Peiman Brouki Milan, Sepideh Hamzehlou, Mansooreh Soleimani, Mohammad Taghi Joghataei, Mazaher Gholipourmalekabadi, Alireza Korourian, Kazem Mousavizadeh, Alexander M Seifalian
Bone regeneration is considered as an unmet clinical need, the aim of this study is to investigate the osteogenic potential of three different mesenchymal stem cells (MSCs) derived from human bone marrow (BM-MSCs), umbilical cord Wharton's jelly (UC-MSCs), and adipose (AD-MSCs) seeded on a recently developed nanocomposite scaffold (bioactive glass/gelatin) implanted in rat animal models with critical size calvarial defects. In this study, after isolation, culture, and characterization, the MSCs were expanded and seeded on the scaffolds for in vitro and in vivo studies...
November 15, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27859871/antheraea-pernyi-silk-sericin-mediating-biomimetic-nucleation-and-growth-of-hydroxylapatite-crystals-promoting-bone-matrix-formation
#13
Zhuang Jiayao, Zhou Guanshan, Zhang Jinchi, Chen Yuyin, Zhu Yongqiang
Bone biomineralization is well-regulated processes mediated by extracellular matrix proteins. The materials that can direct nucleation of hydroxylapatite (HAp) crystals and assembly of well-structured material-minerals complex are the key to mimicking the natural mineralization. This study used sericin from Antheraea pernyi (A.pernyi), non-mulberry silkworm cocoon as template to mediate nucleation of HAp crystals. Here we find out that AS (Antheraea pernyi sericin) can nucleate the formation HAp crystals in simulated body fluid verified by XRD and FTIR observations...
November 18, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27848246/mir-2861-is-involved-in-osteogenic-commitment-of-human-periodontal-ligament-stem-cells-grown-onto-3d-scaffold
#14
F Diomede, I Merciaro, S Martinotti, M Cavalcanti, S Caputi, E Mazzon, O Trubiani
miR-2861 endorsing osteoblast differentiation through the overexpression of Runt-related transcription factor 2 (RUNX2) protein has been recently described. In this study we evaluated: the performance of living construct, composed by human Periodontal Ligament Stem Cells (hPDLSCs) and 3D scaffold (EXg), and the behaviour of miR-2861/RUNX2 expression pathway on the osteogenic commitment. Human PDLSCs were seeded with and without EXg scaffold and cultured under basal and osteogenic conditions. Morphological features, adhesiveness and differentiation abilities were analysed using scanning electron and confocal laser scanning microscopy...
November 16, 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/27835883/ranbpm-ranbp9-regulates-mouse-c-kit-receptor-level-and-is-essential-for-normal-development-of-bone-marrow-progenitor-cells
#15
Sandrine Puverel, Erkan Kiris, Satyendra Singh, Kimberly D Klarmann, Vincenzo Coppola, Jonathan R Keller, Lino Tessarollo
c-Kit is a tyrosine kinase receptor important for gametogenesis, hematopoiesis, melanogenesis and mast cell biology. Dysregulation of c-Kit function is oncogenic and its expression in the stem cell niche of a number of tissues has underlined its relevance for regenerative medicine and hematopoietic stem cell biology. Yet, very little is known about the mechanisms that control c-Kit protein levels. Here we show that the RanBPM/RanBP9 scaffold protein binds to c-Kit and is necessary for normal c-Kit protein expression in the mouse testis and subset lineages of the hematopoietic system...
November 8, 2016: Oncotarget
https://www.readbyqxmd.com/read/27824330/effect-of-sustained-pdgf-non-viral-gene-delivery-on-repair-of-tooth-supporting-bone-defects
#16
A B Plonka, B Khorsand, N Yu, J V Sugai, A K Salem, W V Giannobile, S Elangovan
Recombinant human platelet-derived growth factor-BB (rhPDGF-BB) promotes soft tissue and bone healing, and is FDA-approved for treatment of diabetic ulcers and periodontal defects. The short half-life of topical rhPDGF-BB protein application necessitates bolus, high-dose delivery. Gene therapy enables sustained local growth factor production. A novel gene activated matrix delivering polyplexes of polyethylenimine (PEI)-plasmid DNA (pDNA) encoding PDGF was evaluated for promotion of periodontal wound repair in vivo...
November 8, 2016: Gene Therapy
https://www.readbyqxmd.com/read/27818910/biologically-inspired-smart-release-system-based-on-3d-bioprinted-perfused-scaffold-for-vascularized-tissue-regeneration
#17
Haitao Cui, Wei Zhu, Benjamin Holmes, Lijie Grace Zhang
A critical challenge to the development of large-scale artificial tissue grafts for defect reconstruction is vascularization of the tissue construct. As an emerging tissue/organ manufacturing technique, 3D bioprinting offers great precision in controlling the internal architecture of a scaffold with preferable mechanical strength and printing complicated microstructures comparable to native tissue. However, current bioprinting techniques still exhibit difficulty in achieving biomimetic nano resolution and cooperating with bioactive spatiotemporal signals...
August 2016: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/27815469/effect-of-bmp2-platelet-rich-plasma-biphasic-calcium-phosphate-scaffold-on-accelerated-osteogenesis-in-mastoid-obliteration
#18
Chul Ho Jang, Cheol Hee Choi, Yong Beom Cho
The aim of this study was to evaluate the synergistic effect of platelet-rich plasma (PRP) and recombinant human bone morphogenic protein (BMP)-2 on accelerated osteogenesis of hydroxyapatite/β-tricalcium phosphate mixture and biphasic calcium phosphate (BCP) in mastoid obliteration. To the best of our knowledge, there have been no studies reporting the enhancing effects of BCP, combined with BMP2 and PRP, on osteogenesis in mastoid obliteration. Mastoid obliteration was performed in a control group (BCP only, n=7), a group treated with BMP2 and BCP (experimental group I, n=7), and a group treated with BMP2, PRP and BCP (experimental group II, n=7)...
November 2016: In Vivo
https://www.readbyqxmd.com/read/27813317/bone-forming-cells-with-pronounced-spread-into-the-third-dimension-in-polymer-scaffolds-fabricated-by-two-photon-polymerization
#19
J Heitz, C Plamadeala, M Wiesbauer, P Freudenthaler, R Wollhofen, J Jacak, T A Klar, B Magnus, D Köstner, A Weth, W Baumgartner, R Marksteiner
The main aim of this work was to stimulate bone-forming cells to produce three-dimensional networks of mineralized proteins such as those occurring in bones. This was achieved by a novel approach using a specific type of mesenchymal progenitor cells (i.e., primary fibroblast cells from human hair roots) seeded onto polymer scaffolds. We wrote polymer microstructures with one or more levels of quadratic pores onto a flexible substrate by means of two-photon polymerization using a Ti-sapphire femtosecond laser focused into a liquid acrylate-based resin containing a photoinitiator...
November 3, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27811389/hydrogel-fibers-encapsulating-human-stem-cells-in-an-injectable-calcium-phosphate-scaffold-for-bone-tissue-engineering
#20
Lin Wang, Ping Wang, Michael D Weir, Mark A Reynolds, Liang Zhao, Hockin H K Xu
Human induced pluripotent stem cells (hiPSCs), human embryonic stem cells (hESCs) and human umbilical cord mesenchymal stem cells (hUCMSCs) are exciting cell sources for use in regenerative medicine. There have been no reports on long hydrogel fibers encapsulating stem cells inside an injectable calcium phosphate cement (CPC) scaffold for bone tissue engineering. The objectives of this study were: (1) to develop a novel injectable CPC construct containing hydrogel fibers encapsulating cells for bone engineering, and (2) to investigate and compare cell viability, proliferation and osteogenic differentiation of hiPSC-MSCs, hESC-MSCs and hUCMSCs in injectable CPC...
November 4, 2016: Biomedical Materials
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