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Human bone scaffold

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https://www.readbyqxmd.com/read/28326561/hemoxcell-a-new-oxygen-carrier-usable-as-an-additive-for-mesenchymal-stem-cell-culture-in-platelet-lysate-supplemented-media
#1
Fiona Le Pape, Lucie Cosnuau-Kemmat, Gaëlle Richard, Frédéric Dubrana, Claude Férec, Franck Zal, Elisabeth Leize, Pascal Delépine
Human mesenchymal stem cells (MSCs) are promising candidates for therapeutic applications such as tissue engineering. However, one of the main challenges is to improve oxygen supply to hypoxic areas to reduce oxygen gradient formation while preserving MSC differentiation potential and viability. For this purpose, a marine hemoglobin, HEMOXCell, was evaluated as an oxygen carrier for culturing human bone marrow MSCs in vitro for future three-dimensional culture applications. Impact of HEMOXCell on cell growth and viability was assessed in human platelet lysate (hPL)-supplemented media...
March 22, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28325318/preparation-and-characterization-of-amine-functional-nano-hydroxyapatite-chitosan-bionanocomposite-for-bone-tissue-engineering-applications
#2
Besir Hakan Atak, Berna Buyuk, Merve Huysal, Sevim Isik, Mehmet Senel, Wolfgang Metzger, Guven Cetin
In this study, three different types of scaffolds including a uniquely modified composite scaffold - namely chitosan (CTS), nano-hydroxyapatite/chitosan composite (CTS+nHAP), and amine group (NH2) modified nano-hydroxyapatite/chitosan composite (CTS+nHAP-NH2) scaffolds - were synthesized for bone tissue engineering (BTE) purposes. As results of the study, it was found that all scaffold types were biodegradable with CTS and CTS+nHAP scaffolds losing up to 15% of their initial weight, while the CTS+nHAP-NH2 scaffold showing 10% of weight loss after six weeks of lysozyme treatment...
May 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28321991/atomic-layer-deposition-of-a-silver-nanolayer-on-advanced-titanium-orthopedic-implants-inhibits-bacterial-colonization-and-supports-vascularized-de-novo-bone-ingrowth
#3
Aine Devlin-Mullin, Naomi M Todd, Zahra Golrokhi, Hua Geng, Moritz A Konerding, Nigel G Ternan, John A Hunt, Richard J Potter, Chris Sutcliffe, Eric Jones, Peter D Lee, Christopher A Mitchell
Joint replacement surgery is associated with significant morbidity and mortality following infection with either methicillin-resistant Staphylococcus aureus (MRSA) or Staphylococcus epidermidis. These organisms have strong biofilm-forming capability in deep wounds and on prosthetic surfaces, with 10(3) -10(4) microbes resulting in clinically significant infections. To inhibit biofilm formation, we developed 3D titanium structures using selective laser melting and then coated them with a silver nanolayer using atomic layer deposition...
March 21, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28319248/cyclic-tensile-strain-enhances-human-mesenchymal-stem-cell-smad-2-3-activation-and-tenogenic-differentiation-in-anisotropic-collagen-glycosaminoglycan-scaffolds
#4
W G Grier, A S Moy, B A Harley
Stem cell research arose from the need to explore new therapeutic possibilities for intractable and lethal diseases. Although musculoskeletal disorders are basically nonlethal, their high prevalence and relative ease of performing clinical trials have facilitated the clinical application of stem cells in this field. However, few reliable clinical studies have been published, despite the plethora of in vitro and preclinical studies in stem cell research for regenerative medicine in the musculoskeletal system...
March 20, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28315764/development-of-nanocomposite-scaffolds-based-on-tio2-doped-in-grafted-chitosan-hydroxyapatite-by-freeze-drying-method-and-evaluation-of-biocompatibility
#5
Saber Abd-Khorsand, Samaneh Saber-Samandari, Saeed Saber-Samandari
Porous three-dimensional scaffolds with potential for application as cancellous bone graft substitutes were prepared using the freeze-drying technique. Hydroxyapatite with different weight ratio were embedded in the network of poly(acrylic acid) grafted chitosan accompanied by using TiO2 as an auxiliary component to fabricate porous nanocomposite bone scaffolds. Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction analysis and mechanical tests were carried out to characterize the prepared scaffolds...
March 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28302946/influence-of-lyophilization-factors-and-gelatin-concentration-on-pore-structures-of-atelocollagen-gelatin-sponge-biomaterial
#6
Longqiang Yang, Koji Tanabe, Tadashi Miura, Masao Yoshinari, Shinji Takemoto, Seikou Shintani, Masataka Kasahara
This study aimed to investigate influences of lyophilization factors and gelatin concentration on pore structures of ACG sponge. ACG sponges of different freezing temperatures (-30, -80 and -196(o)C), freezing times (1, 2 and 24 h), gelatin concentrations (0.6%AC+0.15%G, 0.6%AC+0.6%G and 0.6%AC+2.4%G), and with 500 μM fluvastatin were fabricated. Pore structures including porosity and pore size were analyzed by scanning electron microscopy and ImageJ. The cytotoxic effects of ACG sponges were evaluated in vitro...
March 8, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28291964/application-of-a-parallelizable-perfusion-bioreactor-for-physiologic-3d-cell-culture
#7
Dominik Egger, Sarah Spitz, Monica Fischer, Stephan Handschuh, Martin Glösmann, Benedikt Friemert, Monika Egerbacher, Cornelia Kasper
It is crucial but challenging to keep physiologic conditions during the cultivation of 3D cell scaffold constructs for the optimization of 3D cell culture processes. Therefore, we demonstrate the benefits of a recently developed miniaturized perfusion bioreactor together with a specialized incubator system that allows for the cultivation of multiple samples while screening different conditions. Hence, a decellularized bone matrix was tested towards its suitability for 3D osteogenic differentiation under flow perfusion conditions...
March 15, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28287922/co-seeding-human-endothelial-cells-with-human-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells-on-calcium-phosphate-scaffold-enhances-osteogenesis-and-vascularization-in-rats
#8
Xian Liu, Wenchuan Chen, Chi Zhang, Wahwah Thein-Han, Kevin Hu, Mark A Reynolds, Chongyun Bao, Ping Wang, Liang Zhao, Hockin H K Xu
A major challenge in repairing large bone defects with tissue-engineered constructs is the poor vascularization in the defect. The lack of vascular networks leads to insufficient oxygen and nutrients supply, which compromises the survival of seeded cells. To achieve favorable regenerative effects, prevascularization of tissue-engineered constructs by co-culturing of endothelial cells and bone cells is a promising strategy. The aim of this study was to investigate the effects of human-induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs) co-cultured with human umbilical vein endothelial cells (HUVECs) for prevascularization of calcium phosphate cement (CPC) scaffold on bone regeneration in vivo for the first time...
March 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28287912/osteogenic-differentiation-of-mesenchymal-stromal-cells-a-comparative-analysis-between-human-subcutaneous-adipose-tissue-and-dental-pulp
#9
Iolanda D'Alimonte, Filiberto Mastrangelo, Patricia Giuliani, Laura Pierdomenico, Marco Marchisio, Mariachiara Zuccarini, Patrizia Di Iorio, Raimondo Quaresima, Francesco Caciagli, Renata Ciccarelli
White adipose tissue is a source of mesenchymal stromal/stem cells (MSCs) that are actively studied for their possible therapeutic use in bone tissue repair/remodeling. To better appreciate the osteogenic potential of these cells, we compared some properties of MSCs from human subcutaneous adipose tissue [subcutaneous-adipose stromal cells (S-ASCs)] and dental pulp stem cell (DPSCs) of third-impacted molars, the latter representing a well-established MSC source. Both undifferentiated cell types showed similar fibroblast-like morphology and mesenchymal marker expression...
March 13, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28286037/surface-enrichment-with-hydroxyapatite-nanoparticles-in-stereolithography-fabricated-composite-polymer-scaffolds-promotes-bone-repair
#10
O Guillaume, M A Geven, C M Sprecher, V A Stadelmann, D W Grijpma, T T Tang, L Qin, Y Lai, M Alini, J D de Bruijn, H Yuan, R G Richards, D Eglin
Fabrication of composite scaffolds using stereolithography (SLA) for bone tissue engineering has shown great promises. However, in order to trigger effective bone formation and implant integration, exogenous growth factors are commonly combined to scaffold materials. In this study, we fabricated biodegradable composite scaffolds using SLA and endowed them with osteopromotive properties in the absence of biologics. First we prepared photo-crosslinkable poly(trimethylene carbonate) (PTMC) resins containing 20 and 40 wt...
March 7, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28285576/decellularized-bone-extracellular-matrix-and-human-dental-pulp-stem-cells-as-a-construct-for-bone-regeneration
#11
Francesco Paduano, Massimo Marrelli, Noura Alom, Mahetab Amer, Lisa J White, Kevin M Shakesheff, Marco Tatullo
Dental pulp tissue represents a source of mesenchymal stem cells that have a strong differentiation potential towards the osteogenic lineage. The objective of the current study was to examine in vitro osteogenic induction of dental pulp stem cells (DPSCs) cultured on hydrogel scaffolds derived from decellularized bone extracellular matrix (bECM) compared to collagen type I (Col-I), the major component of bone matrix. DPSCs in combination with bECM hydrogels were cultured under three different conditions: basal medium, osteogenic medium and medium supplemented with growth factors (GFs) and cell growth, mineral deposition, gene and protein expression were investigated...
March 13, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28280669/in-vivo-chondrogenesis-in-3d-bioprinted-human-cell-laden-hydrogel-constructs
#12
Thomas Möller, Matteo Amoroso, Daniel Hägg, Camilla Brantsing, Nicole Rotter, Peter Apelgren, Anders Lindahl, Lars Kölby, Paul Gatenholm
BACKGROUND: The three-dimensional (3D) bioprinting technology allows creation of 3D constructs in a layer-by-layer fashion utilizing biologically relevant materials such as biopolymers and cells. The aim of this study is to investigate the use of 3D bioprinting in a clinically relevant setting to evaluate the potential of this technique for in vivo chondrogenesis. METHODS: Thirty-six nude mice (Balb-C, female) received a 5- × 5- × 1-mm piece of bioprinted cell-laden nanofibrillated cellulose/alginate construct in a subcutaneous pocket...
February 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28279182/the-use-of-mesenchymal-stem-cells-for-cartilage-repair-and-regeneration-a-systematic-review
#13
REVIEW
Andy Goldberg, Katrina Mitchell, Julian Soans, Louise Kim, Razi Zaidi
BACKGROUND: The management of articular cartilage defects presents many clinical challenges due to its avascular, aneural and alymphatic nature. Bone marrow stimulation techniques, such as microfracture, are the most frequently used method in clinical practice however the resulting mixed fibrocartilage tissue which is inferior to native hyaline cartilage. Other methods have shown promise but are far from perfect. There is an unmet need and growing interest in regenerative medicine and tissue engineering to improve the outcome for patients requiring cartilage repair...
March 9, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28276640/prospective-review-of-mesenchymal-stem-cells-differentiation-into-osteoblasts
#14
REVIEW
Priyanka Garg, Matthew M Mazur, Amy C Buck, Meghan E Wandtke, Jiayong Liu, Nabil A Ebraheim
Stem cell research has been a popular topic in the past few decades. This review aims to discuss factors that help regulate, induce, and enhance mesenchymal stem cell (MSC) differentiation into osteoblasts for bone regeneration. The factors analyzed include bone morphogenic protein (BMP), transforming growth factor β (TGF-β), stromal cell-derived factor 1 (SDF-1), insulin-like growth factor type 1 (IGF-1), histone demethylase JMJD3, cyclin dependent kinase 1 (CDK1), fucoidan, Runx2 transcription factor, and TAZ transcriptional coactivator...
March 9, 2017: Orthopaedic Surgery
https://www.readbyqxmd.com/read/28267421/use-of-pig-as-a-model-for-mesenchymal-stem-cell-therapies-for-bone-regeneration
#15
Marcello Rubessa, Kathryn Polkoff, Massimo Bionaz, Elisa Monaco, Derek J Milner, Scott J Holllister, Michael S Goldwasser, Matthew B Wheeler
Bone is a plastic tissue with a large healing capability. However, extensive bone loss due to disease or trauma requires extreme therapy such as bone grafting or tissue-engineering applications. Presently, bone grafting is the gold standard for bone repair, but presents serious limitations including donor site morbidity, rejection, and limited tissue regeneration. The use of stem cells appears to be a means to overcome such limitations. Bone marrow mesenchymal stem cells (BMSC) have been the choice thus far for stem cell therapy for bone regeneration...
March 7, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28266827/incorporation-of-human-platelet-derived-growth-factor-bb-encapsulated-poly-lactic-co-glycolic-acid-microspheres-into-3d-coragraf-enhances-osteogenic-differentiation-of-mesenchymal-stromal-cells
#16
Saktiswaren Mohan, Hanumantharao Balaji Raghavendran, Puvanan Karunanithi, Malliga Raman Murali, Sangeetha Vasudevaraj Naveen, Sepehr Talebian, Mohammad Mehrali, Mehdi Mehrali, Elango Natarajan, Chee Ken Chan, Tunku Kamarul
Tissue engineering aims to generate or facilitate regrowth or healing of damaged tissues by applying a combination of biomaterials, cells, and bioactive signaling molecules. In this regard, growth factors clearly play important roles in regulating cellular fate. However, uncontrolled release of growth factors has been demonstrated to produce severe side effects on the surrounding tissues. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres (MS) incorporated three-dimensional (3D) CORAGRAF scaffolds were engineered to achieve controlled release of platelet-derived growth factor-BB (PDGF-BB) for the differentiation of stem cells within the 3D polymer network...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28263431/electrospun-oriented-gelatin-hydroxyapatite-fibre-scaffolds-for-bone-tissue-engineering
#17
Ali A Salifu, Constantina Lekakou, Fatima Labeed
Tissue engineering of human foetal osteoblast (hFOB) cells was investigated on gelatin-hydroxyapatite (HA), crosslinked, electrospun oriented fibre scaffolds at the different hydroxyapatite concentrations of 0, 10, 20 and 25 wt% in the dry fibres and different fibre diameter, pore size and porosity of scaffolds. Rheological tests and proton NMR spectroscopy were conducted for all solutions used for electrospinning. It was found that 25 wt% HA-gelatin scaffolds electrospun at 20 kV led to the greatest cell attachment, cell proliferation and extracellular matrix (ECM) production while fibre orientation improved the mechanical properties, where crosslinked electrospun 25 wt% HA-gelatin fibre scaffolds yielded a Young's modulus in the range of 0...
March 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28260604/the-osteogenic-potential-of-human-nondifferentiated-and-pre-differentiated-mesenchymal-stem-cells-combined-with-an-osteoconductive-scaffold-early-stage-healing
#18
Luboš Tuček, Zuzana Kočí, Kristýna Kárová, Helena Doležalová, Jakub Suchánek
Despite the huge research into stem cells and their regenerative properties for bone healing, there are still unanswered questions including the recipient's respond to the presence of the stem cells, the fate of stem cells inside the bone defect and the possible advantage in utilizing pre-differentiated cells. To address these problems, we used human multipotent mesenchymal stromal/stem cells (MSCs), GMP Grade, in a rat model of bone formation. In a "bioreactor concept" approach seven Wistar rats were implanted with 0...
March 6, 2017: Acta Medica (Hradec Králové)
https://www.readbyqxmd.com/read/28256634/monomeric-porous-type-ii-collagen-scaffolds-promote-chondrogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-in-vitro
#19
M Tamaddon, M Burrows, S A Ferreira, F Dazzi, J F Apperley, A Bradshaw, D D Brand, J Czernuszka, E Gentleman
Osteoarthritis (OA) is a common cause of pain and disability and is often associated with the degeneration of articular cartilage. Lesions to the articular surface, which are thought to progress to OA, have the potential to be repaired using tissue engineering strategies; however, it remains challenging to instruct cell differentiation within a scaffold to produce tissue with appropriate structural, chemical and mechanical properties. We aimed to address this by driving progenitor cells to adopt a chondrogenic phenotype through the tailoring of scaffold composition and physical properties...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28254319/evaluation-of-bioactive-glass-incorporated-poly-caprolactone-poly-vinyl-alcohol-matrix-and-the-effect-of-bmp-2-modification
#20
Khamsone Keothongkham, Narattaphol Charoenphandhu, Jirawan Thongbunchoo, Panan Suntornsaratoon, Nateetip Krishnamra, I-Ming Tang, Weeraphat Pon-On
Composite materials having mechanical and biological properties similar to those of human bones are needed for bone regeneration and repair. In the present study, composites were made by incorporating bioactive glass (BG) into polycaprolactone (PCL)-polyvinyl alcohol (PVA) (PCLPVA) matrix. Composites with different BG contents of 10, 25 and 50wt% were fabricated by an in-situ blending method. Physicochemical properties measurements found that the composite with 50wt% BG in the PCLPVA organic matrix exhibited the best mechanical properties (compressive strength and compressive young's modulus up to 32...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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