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Human bone scaffold

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https://www.readbyqxmd.com/read/28718708/enhanced-bone-repair-induced-by-human-adipose-derived-stem-cells-on-osteogenic-extracellular-matrix-ornamented-small-intestinal-submucosa
#1
Chi Zhang, Mei Li, Jinjin Zhu, Fangmiao Luo, Jiyuan Zhao
AIM: Our aim was to design an osteogenic extracellular matrix (ECM) coated bioscaffold and to apply it to critical bone defect repair with adipose-derived stem cells (ADSCs). MATERIALS & METHODS: Morphology of scaffolds was scanned by scanning electron microscope. Cell adhesion, proliferation and osteogenic differentiation of ADSCs on ECM-small intestinal submucosa (SIS) were evaluated by immunofluorescences staining, cell counting kit-8 and real-time qPCR, respectively...
July 18, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28717129/3d-printed-scaffolds-of-calcium-silicate-doped-%C3%AE-tcp-synergize-with-co-cultured-endothelial-and-stromal-cells-to-promote-vascularization-and-bone-formation
#2
Yuan Deng, Chuan Jiang, Cuidi Li, Tao Li, Mingzheng Peng, Jinwu Wang, Kerong Dai
Synthetic bone scaffolds have potential application in repairing large bone defects, however, inefficient vascularization after implantation remains the major issue of graft failure. Herein, porous β-tricalcium phosphate (β-TCP) scaffolds with calcium silicate (CS) were 3D printed, and pre-seeded with co-cultured human umbilical cord vein endothelial cells (HUVECs) and human bone marrow stromal cells (hBMSCs) to construct tissue engineering scaffolds with accelerated vascularization and better bone formation...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28714856/use-of-human-aortic-extracellular-matrix-as-a-scaffold-for-construction-of-patient-specific-tissue-engineered-vascular-patch
#3
Liping Gao, Mingjun Du, Jingjing Lv, Sebastian Schmull, Ritai Huang, Jun Li
Abstract Synthetic or biologic materials are usually used to repair vascular malformation in congenital heart defects; however, non-autologous materials show both mismatch compliance and antigenicity, as well as a lack of recellularization on its surface. Here, we constructed a tissue-engineered vascular patch (TEVP) using decellularized extracellular matrix (ECM) scaffold obtained from excised human aorta during surgery, which was seeded with patient-derived bone marrow CD34-positive (CD34+) progenitor cells...
July 17, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28713741/reconstruction-of-drug-induced-cleft-palate-using-bone-marrow-mesenchymal-stem-cell-in-rodents
#4
Julie Christy Amalraj, Manasa Gangothri, Hari Babu
BACKGROUND: Triamcinolone acetonide (TAC) (Kenacort*) is a commonly used synthetic glucocorticoid in today's medical practice. The drug is also a potential agent in inducing cleft palates in rats. This drug has been used to induce cleft palate in the fetus of the pregnant rats to bring out a suitable animal model for human cleft lip and palate. The drug was given intraperitoneally to induce congenital cleft palate in pregnant mother rats. AIM: The aim of this study is to induce congenital cleft palate in pregnant Wister albino rats and reconstruct the defect with bone marrow mesenchymal stem cells (BMSCs) isolated from the same species along with PLGA (poly lactic co glycolic acid) scaffold...
January 2017: Annals of Maxillofacial Surgery
https://www.readbyqxmd.com/read/28690010/tailoring-surface-nanoroughness-of-electrospun-scaffolds-for-skeletal-tissue-engineering
#5
Honglin Chen, Xiaobin Huang, Minmin Zhang, Febriyani Damanik, Matthew B Baker, Anne Leferink, Huipin Yuan, Roman Truckenmüller, Clemens van Blitterswijk, Lorenzo Moroni
Electrospun scaffolds provide a promising approach for tissue engineering as they mimic the physical properties of extracellular matrix. Previous studies have demonstrated that electrospun scaffolds with porous features on the surface of single fibers, enhanced cellular attachment and proliferation. Yet, little is known about the effect of such topographical cues on cellular differentiation. Here, we aimed at investigating the influence of surface roughness of electrospun scaffolds on skeletal differentiation of human mesenchymal stromal cells (hMSCs)...
July 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28686698/flow-velocity-driven-differentiation-of-human-mesenchymal-stromal-cells-in-silk-fibroin-scaffolds-a-combined-experimental-and-computational-approach
#6
Jolanda Rita Vetsch, Duncan Colin Betts, Ralph Müller, Sandra Hofmann
Mechanical loading plays a major role in bone remodeling and fracture healing. Mimicking the concept of mechanical loading of bone has been widely studied in bone tissue engineering by perfusion cultures. Nevertheless, there is still debate regarding the in-vitro mechanical stimulation regime. This study aims at investigating the effect of two different flow rates (vlow = 0.001m/s and vhigh = 0.061m/s) on the growth of mineralized tissue produced by human mesenchymal stromal cells cultured on 3-D silk fibroin scaffolds...
2017: PloS One
https://www.readbyqxmd.com/read/28672271/the-fabrication-and-characterization-of-barium-titanate-akermanite-nano-bio-ceramic-with-a-suitable-piezoelectric-coefficient-for-bone-defect-recovery
#7
H Shokrollahi, F Salimi, A Doostmohammadi
In recent years, due to the controllable mechanical properties and degradation rate, calcium silicates such as akermanite (Ca2MgSi2O7) with Ca-Mg and Si- containing bio-ceramics have received much more attention. In addition, the piezoelectric effect plays an important role in bone growth, remodeling and defect healing. To achieve our objective, the porous bioactive nano-composite with a suitable piezoelectric coefficient was fabricated by the freeze-casting technique from the barium titanate and nano-akermanite (BT/nAK) suspension...
June 22, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28668476/conventional-and-microwave-assisted-synthesis-of-new-1h-benzimidazole-thiazolidinedione-derivatives-a-potential-anticancer-scaffold
#8
Pankaj Sharma, T Srinivasa Reddy, Niggula Praveen Kumar, Kishna Ram Senwar, Suresh K Bhargava, Nagula Shankaraiah
A series of new benzimidazole bearing thiazolidinedione derivatives has been designed, synthesized by using conventional as well as microwave-assisted methods. Microwave-assisted synthesis caused a significant reduction in the reaction times and improvement in the yields of all the derivatives. All the new synthesized compounds were evaluated for their in vitro cytotoxic potential against selected human cancer cell lines of breast (MDAMB-231), prostate (PC-3), cervical (HeLa), lung (A549) and bone (HT1080) along with a normal kidney cells (HeK-293T)...
June 23, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28661587/endochondral-ossification-in-critical-sized-bone-defects-via-readily-implantable-scaffold-free-stem-cell-constructs
#9
Phuong N Dang, Samuel Herberg, Davood Varghai, Hooman Riazi, Daniel Varghai, Alexandra McMillan, Amad Awadallah, Lauren M Phillips, Oju Jeon, Minh K Nguyen, Neha Dwivedi, Xiaohua Yu, William L Murphy, Eben Alsberg
The growing socioeconomic burden of musculoskeletal injuries and limitations of current therapies have motivated tissue engineering approaches to generate functional tissues to aid in defect healing. A readily implantable scaffold-free system comprised of human bone marrow-derived mesenchymal stem cells embedded with bioactive microparticles capable of controlled delivery of transforming growth factor-beta 1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) was engineered to guide endochondral bone formation...
July 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28659417/human-adipose-stem-cells-induced-to-osteogenic-differentiation-by-an-innovative-collagen-hydroxylapatite-hybrid-scaffold
#10
Elisa Mazzoni, Antonio D'Agostino, Marco Manfrini, Stefania Maniero, Andrea Puozzo, Elena Bassi, Stefano Marsico, Cinzia Fortini, Lorenzo Trevisiol, Simone Patergnani, Mauro Tognon
Novel biomaterials are of paramount importance for bone regrowth. In this study, we investigated human adipose stem cells (hASCs) for osteogenic, osteoconductivity, and osteoinductivity effects of an innovative collagen/hydroxylapatite hybrid scaffold. In hASCs that were grown on this scaffold, osteogenic genes were analyzed for their expression profiles, together with adhesion and extracellular matrix genes. In hASC integrins, basement membrane constituents and collagens were up-regulated, together with cell proliferation...
June 28, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28658717/bone-marrow-on-a-chip-long-term-culture-of-human-hematopoietic-stem-cells-in-a-3d-microfluidic-environment
#11
Stefan Sieber, Lorenz Wirth, Nino Cavak, Marielle Koenigsmark, Uwe Marx, Roland Lauster, Mark Rosowski
Multipotent hematopoietic stem and progenitor cells (HSPCs) are the source for all blood cell types. The bone marrow stem cell niche in which the HSPCs are maintained is known to be vital for their maintenance. Unfortunately, to this date no in vitro model exists that truthfully mimics the aspects of the bone marrow niche and simultaneously allows the long-term culture of HSPCs. In this study, we present a novel 3D co-culture model, based on a hydroxyapatite coated zirconium oxide scaffold, comprising of human mesenchymal stromal cells (MSCs) and cord blood derived HSPCs, enabling successful HSPC culture for a time span of 28 days within the microfluidic Multi-Organ-Chip (MOC)...
June 28, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28652926/silk-fibroin-hydroxyapatite-composite-hydrogel-induced-by-gamma-ray-irradiation-for-bone-tissue-engineering
#12
Min Hee Kim, Beom Su Kim, Jun Lee, Donghwan Cho, Oh Hyeong Kwon, Won Ho Park
BACKGROUND: In this study, silk fibroin (SF) composite hydrogels containing hydroxyapatite (HAP) nanoparticles (NPs) for bone tissue engineering were fabricated using gamma-ray (γ-ray) irradiation treatment. During the irradiation, the HAP dispersed SF solution was changed to the chemically crosslinked SF hydrogel. METHODS: Distribution of HAP NPs in the SF hydrogel was examined by SEM imagery and energy dispersive X-ray spectrophotometry, and the crystalline structure of SF composite hydrogels was also confirmed by X-ray diffractometry...
2017: Biomaterials Research
https://www.readbyqxmd.com/read/28647591/chitosan-based-nanocomposites-for-the-repair-of-bone-defects
#13
L Keller, A Regiel-Futyra, M Gimeno, S Eap, G Mendoza, V Andreu, Q Wagner, A Kyzioł, V Sebastian, G Stochel, M Arruebo, N Benkirane-Jessel
Chitosan scaffolds of different deacetylation degrees, average molecular weights and concentrations reinforced with silica nanoparticles were prepared for bone tissue regeneration. The resulting nanocomposites showed similar pore sizes (<300 μm) regardless the deacetylation degree and concentration used in their formulation. Their mechanical compression resistance was increased by a 30% with the addition of silica nanoparticles as nanofillers. The biocompatibility of the three-dimensional chitosan scaffolds was confirmed by the Alamar Blue assay in human primary osteoblasts as well as the formation of cell spheroids indicative of their great potential for bone regeneration...
June 22, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28646917/polycaprolactone-nanofiber-scaffold-enhances-the-osteogenic-differentiation-potency-of-various-human-tissue-derived-mesenchymal-stem-cells
#14
Ruyue Xue, Yuna Qian, Linhao Li, Guidong Yao, Li Yang, Yingpu Sun
BACKGROUND: Polycaprolactone (PCL) has been regarded as a promising synthetic material for bone tissue engineering application. Owing to its unique biochemical properties and great compatibility, PCL fibers have come to be explored as a potential delivering scaffold for stem cells to support bone regeneration during clinical application. METHODS: The human derived mesenchymal stem cells (MSCs) were obtained from umbilical cord (UC), bone marrow (BM), and adipose tissue (AD), respectively...
June 24, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28643418/protein-engineering-of-recombinant-human-bone-morphogenetic-protein-2-with-higher-interaction-with-ca-phosphate-based-scaffold-usedfor-osteogenesis
#15
Mohammad Bayat, Sahar Shojaei, Naghmeh Bahrami, Abdol Reza Mohamadnia, Pegah Shojaei, Naeim Bahrami
The aim of the present studywas to assess the recombinant bonemorphogenetic protein 2 (RHBMP-2)with higher substantively and solubility for use in calcium phosphate scaffolds for better release in differentiation of mesenchymal stem cells to osteoblast cells. By using bioinformatics tools,2 mutations (p. L10D and p. S12E) werechosen and applied in BMP2 CDS sequenceto increase interaction with calcium derived composite. The new recombinant mutated sequence (BMP2(mut) ) was synthesized and then subcloned to expression vector pBV220...
June 23, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28639378/regulation-of-the-fate-of-dental-derived-mesenchymal-stem-cells-using-engineered-alginate-gelma-hydrogels
#16
Sahar Ansari, Patricia Sarrion, Mohammad Mahdi Hasani-Sadrabdi, Tara Aghaloo, Benjamin M Wu, Alireza Moshaverinia
Mesenchymal stem cells (MSCs) derived from dental and orofacial tissues provide an alternative therapeutic option for craniofacial bone tissue regeneration. However, there is still a need to improve stem cell delivery vehicles to regulate the fate of the encapsulated MSCs for high quality tissue regeneration. Matrix elasticity plays a vital role in MSC fate determination. Here we have prepared various hydrogel formulations based on alginate and gelatin methacryloyl (GelMA) and have encapsulated gingival mesenchymal stem cells (GMSCs) and human bone marrow MSCs (hBMMSCs) within these fabricated hydrogels...
June 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28636926/enhanced-human-bone-marrow-mesenchymal-stromal-cell-adhesion-on-scaffolds-promotes-cell-survival-and-bone-formation
#17
Miryam Mebarki, Laura Coquelin, Pierre Layrolle, Séverine Battaglia, Marine Tossou, Philippe Hernigou, Hélène Rouard, Nathalie Chevallier
In order to induce an efficient bone formation with human bone marrow mesenchymal stromal cells (hBMSC) associated to a scaffold, it is crucial to determine the key points of the hBMSC action after in vivo transplantation as well as the appropriate features of a scaffold. To this aim we compared the hBMSC behavior when grafted onto two biomaterials allowing different bone potential in vivo. The cancellous devitalized Tutoplast®-processed bone (TPB) and the synthetic hydroxyapatite/β-tricalcium-phosphate (HA/βTCP) which give at 6 weeks 100% and 50% of bone formation respectively...
June 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28635177/engineering-human-bone-grafts-with-new-macroporous-calcium-phosphate-cement-scaffolds
#18
Martina Sladkova, Michael Palmer, Caroline Öhman, Jiayi Cheng, Shoug Al-Ansari, Munerah Saad, Håkan Engqvist, Giuseppe Maria de Peppo
Bone engineering opens the possibility to grow large amounts of tissue products by combining patient-specific cells with compliant biomaterials. Decellularized tissue matrices represent suitable biomaterials but availability, long processing time, excessive cost, and concerns on pathogen transmission have led to the development of biomimetic synthetic alternatives. We recently fabricated calcium phosphate cement (CPC) scaffolds with variable macroporosity using a facile synthesis method with minimal manufacturing steps, and demonstrated long-term biocompatibility in vitro...
June 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28631418/the-effects-of-biodentine-polycaprolactone-3d-scaffold-with-odontogenesis-properties-on-human-dental-pulp-cells
#19
C-C Ho, H-Y Fang, B Wang, T-H Huang, M-Y Shie
AIM: To determine the feasibility of using three-dimensional printed Biodentine/polycaprolactone composite scaffolds for orthopaedic and dental applications. The physicochemical properties and the odontogenic differentiation of human dental pulp cells (hDPCs) were investigated. METHODOLOGY: Biodentine was well-suspended in ethanol and dropped slowly into molten polycaprolactone with vigorous stirring. The Biodentine/polycaprolactone composite scaffolds were then fabricated into controlled macropore sizes and structures using an extrusion-based three-dimensional (3D) printer...
June 20, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28629079/silver-doped-resorbable-tricalcium-phosphate-scaffolds-for-bone-graft-applications
#20
Sean Hoover, Solaiman Tarafder, Amit Bandyopadhyay, Susmita Bose
Bone graft procedures, in particular maxillofacial repair, account for half of the orthopedic procedures done in the US each year. Infection is a major issue in surgery, and should be of primary concern when engineering biomaterials. Silver is of renewed importance today, as it has the ability to potentiate antibiotics against resistant bacterial strains. In order to reduce long term infection risks, it is necessary for the scaffold to maintain a silver ion release for the length of the healing process. In this study, silver doped porous β-tricalcium phosphate (β-TCP) scaffolds were engineered using liquid porogen based method with the goal of meeting these requirements...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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