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https://www.readbyqxmd.com/read/28945015/membrane-fusion-mediated-intracellular-delivery-of-lipid-bilayer-coated-mesoporous-silica-nanoparticles
#1
Jian Yang, Jing Tu, Gerda E M Lamers, René C L Olsthoorn, Alexander Kros
Protein delivery into the cytosol of cells is a challenging topic in the field of nanomedicine, because cellular uptake and endosomal escape are typically inefficient, hampering clinical applications. In this contribution cuboidal mesoporous silica nanoparticles (MSNs) containing disk-shaped cavities with a large pore diameter (10 nm) are studied as a protein delivery vehicle using cytochrome-c (cytC) as a model membrane-impermeable protein. To ensure colloidal stability, the MSNs are coated with a fusogenic lipid bilayer (LB) and cellular uptake is induced by a complementary pair of coiled-coil (CC) lipopeptides...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28945005/differential-expression-of-myometrial-ap-1-proteins-during-gestation-and-labour
#2
Lubna Nadeem, Tali Farine, Anna Dorogin, Elzbieta Matysiak-Zablocki, Oksana Shynlova, Stephen Lye
Preterm labour (PTL) is a leading cause of perinatal mortality and postnatal morbidity. Contractions of the uterine muscle (myometrium) that determine the onset of labour depend on the expression of contraction-associated proteins (CAPs, i.e. connexin43) regulated by dimeric AP-1 transcription factors. Here, we examined subcellular (by immunoblotting) and tissue expression (by immunohistochemistry) of myometrial AP-1 proteins (cJUN, JUNB, JUND, cFOS, FOSB, FRA1, FRA2) throughout gestation and TL in different species (mouse, rat and human)...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944995/comparative-analysis-of-serum-proteome-in-congenital-scoliosis-patients-with-tbx6-haploinsufficiency-a-first-report-pointing-to-lipid-metabolism
#3
Qiankun Zhu, Nan Wu, Gang Liu, Yangzhong Zhou, Sen Liu, Jun Chen, Jiaqi Liu, Yuzhi Zuo, Zhenlei Liu, Weisheng Chen, Yixin Chen, Jia Chen, Mao Lin, Yanxue Zhao, Yang Yang, Shensgru Wang, Xu Yang, Yufen Ma, Jian Wang, Xiaoli Chen, Jianguo Zhang, Jianxiong Shen, Zhihong Wu, Guixing Qiu
Congenital scoliosis (CS) is a three-dimensional deformity of the spine affecting quality of life. We have demonstrated TBX6 haploinsufficiency is the most important contributor to CS. However, the pathophysiology at the protein level remains unclear. Therefore, this study was to explore the differential proteome in serum of CS patients with TBX6 haploinsufficiency. Sera from nine CS patients with TBX6 haploinsufficiency and nine age- and gender-matched healthy controls were collected and analysed by isobaric tagged relative and absolute quantification (iTRAQ) labelling coupled with mass spectrometry (MS)...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944982/evaluation-of-inos-inhibition-on-kidney-function-and-structure-in-high-fat-diet-induced-kidney-disease
#4
Blanche Martin, Nathalie Caron, Inès Jadot, Vanessa Colombaro, Gabrielle Federici, Clara Depommier, Anne-Émilie Declèves
BACKGROUND: Central obesity is related to caloric excess promoting deleterious cellular responses in targeted organs. Nitric oxide (NO) has been determined as a key player in the pathogenesis of metabolic diseases. Here, we investigated the implication of inducible NO synthase (iNOS) in the development of obesity-induced kidney disease. MATERIALS AND METHODS: C57Bl/6 male mice were randomized to a low-fat diet (LFD) or a high-fat diet (HFD) and treated with L-NIL, a specific iNOS inhibitor for 16 weeks...
September 25, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/28944919/proprotein-convertase-subtilisin-kexin-type-6-promotes-in%C3%A2-vitro-proliferation-migration-and-inflammatory-cytokine-secretion-of-synovial-fibroblast%C3%A2-like-cells-from-rheumatoid-arthritis-via-nuclear%C3%A2-%C3%AE%C2%BAb-signal-transducer-and-activator-of-transcription-3-and
#5
Huiyu Jiang, Lin Wang, Feifei Wang, Jihong Pan
Our previous studies demonstrated that expression of proprotein convertase subtilisin/kexin type 6 (PCSK6) is greatly enhanced in rheumatoid arthritis fibroblast‑like synoviocytes (RASFs), and that PCSK6 inhibition decreases cell proliferation, migration and invasion. The present study aimed to investigate the functional role of PCSK6 in the hyperplasia of RASFs. Cultured RASFs from RA patients were stimulated with recombinant human (rh)PCSK6. Subsequent changes in proliferation, invasion, migration and the secretion of inflammatory cytokines were measured in vitro using MTT, wound healing and Transwell assays, and ELISA...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944918/microrna%C3%A2-340-inhibits-tumor-cell-proliferation-migration-and-invasion-and-induces-apoptosis-in-hepatocellular-carcinoma
#6
Ziyao Wang, Dan Song, Ping Huang
MicroRNAs (miRs) are short RNAs that serve a role in the origination and progression of hepatocellular carcinoma (HCC). miR‑340 has been identified to be a novel tumor suppressor. The present study investigated the antitumor function of miR‑340 in HCC. In the present study, it was detected that miR‑340 was significantly decreased in HCC cancer tissues and human HCC cell lines using reverse transcription‑quantitative polymerase chain reaction analysis. Cell Counting kit‑8 and apoptosis assays demonstrated that miR‑340 reduced cell proliferation and induced cellular apoptosis in HCC cell lines...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944891/thioredoxin-is-implicated-in-the-anti%C3%A2-apoptotic-effects-of-grape-seed-proanthocyanidin-extract-during-hyperglycemia
#7
Xiang Ren, Heyuan Lu, Nina Wang, Chenghong Zhang, Yunpeng Ji, Shiqi Cui, Yichen Dong, Kaiyuan Yang, Mengyi Du, Fengsheng Diao, Li Kong
Diabetic retinopathy has long been recognized as a microvascular disease, however, recent research has indicated that diabetic retinopathy may also be considered a neurodegenerative disease. The elucidation of the molecular mechanisms underlying the development of diabetic retinopathy is imperative for the development of preventive and treatment strategies for patients with diabetes. In the present study, grape seed proanthocyanidin extract (GSPE) was used to upregulate the expression of thioredoxin (Trx), in order to evaluate its potential as a novel agent for the prevention and treatment of neurodegenerative diseases, including diabetic retinopathy...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944871/shear-stress-induces-human-aortic-endothelial-cell-apoptosis-via-interleukin%C3%A2-1-receptor%C3%A2-associated-kinase-2%C3%A2-induced-endoplasmic-reticulum-stress
#8
Longfei Pan, Zhou Hong, Lei Yu, Yanxia Gao, Rui Zhang, Hui Feng, Lijuan Su, Gang Wang
Atherosclerosis is characterized by localized lesions distributed in the arterial tree due to the shear stress produced by blood flow. Endothelial cells are directly affected by alterations in blood flow. Dysfunction and injury to endothelial cells has been hypothesized to initiate the pathological processes of atherosclerosis. The present study aimed to investigate the mechanism of shear stress‑induced endothelial cellular apoptosis. Shear stress was generated using an artificial device to mimic the impact of disturbed blood flow on cultured human aortic endothelial cells (HAECs)...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944849/celastrol-alleviates-angiotensin-ii%C3%A2-mediated-vascular-smooth-muscle-cell-senescence-via-induction-of-autophagy
#9
Xian-Jie Xu, Wei-Bo Zhao, Shi-Bin Feng, Cheng Sun, Qiang Chen, Bing Ni, Hou-Yuan Hu
Reactive oxygen species (ROS) production has been implicated in the promotion of cellular senescence. Celastrol, a quinone methide triterpenoid isolated from the Celastraceae family, exerts antioxidant effects and enhances autophagy in various cell types. Since autophagy serves an important role in regulating ROS, it was hypothesized that the antioxidant effect of celastrol is via enhanced autophagy, thus inhibiting cell senescence. Therefore, the present study used a Senescence β‑Galactosidase Staining kit, western blot analysis and cell cycle analysis to investigate whether celastrol alleviates angiotensin (Ang) II‑induced cellular senescence by upregulating autophagy in vascular smooth muscle cells (VSMCs)...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944847/geniposide-promotes-autophagy-to-inhibit-insulin-resistance-in-hepg2-cells-via-p62-nf%C3%A2-%C3%AE%C2%BAb-glut%C3%A2-4
#10
Hongwei Jiang, Yujin Ma, Junqiang Yan, Jie Liu, Liping Li
Insulin resistance (IR) is known to be an important factor, which can lead to the onset of type 2 diabetes. Autophagy is a cellular process, which sequesters senescent or damaged proteins in autophagosomes for recycling of their products. Insulin and intracellular molecules, including mammalian target of rapamycin (mTOR), are well‑known inhibitors of autophagy. In patients with type 2 diabetes, the expression levels of glucose transporter 4 (GLUT‑4) in skeletal muscles are significantly decreased, indicating decreased glucose‑processing ability...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944843/microarray-and-bioinformatics-analyses-of-gene-expression-profiles-in-balb-c-murine-macrophage-polarization
#11
Li Jiang, Xueqin Li, Yingying Zhang, Mengying Zhang, Zongsheng Tang, Kun Lv
Macrophages possess the hallmark feature of plasticity, allowing them to undergo a dynamic transition between M1 and M2 polarized phenotypes. The aim of the present study was to screen for differentially-expressed genes (DEGs) that were associated with BALB/c murine macrophage polarization. The transcription profiles of three M1 and three M2 samples were obtained using microarray analysis. Based on the threshold of fold‑change >2.0 and P‑value <0.05, a total of 1,253 DEGs were identified, of which 696 were upregulated and 557 downregulated in M1 macrophages compared with M2 macrophages...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944838/effect-of-antioxidant-of-bamboo-leaves-on-gene-expression-associated-with-mouse-embryonic-fibroblast-reproduction-and-embryonic-development
#12
Feng Yu, Xiaowei Qian, Zhanghui Zeng, Xiaoli Zhao, Rong Hou, Zhihe Zhang, Hongwu Bian, Ning Han, Junhui Wang, Muyuan Zhu
Antioxidant of bamboo leaves (AOB) was certified to be a natural antioxidant by the Chinese Ministry of Health in 2003. However, the effects of AOB on animal reproductive and developmental functions remain unclear. The present study aimed to investigate the effects of different concentrations of AOB on mouse embryonic fibroblast (MEF) cells, and to examine the underlying molecular mechanism through which AOB affects the proliferation and apoptosis of MEFs. MEFs prepared from individual embryos were treated with various dosages of AOB...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944825/mild-hypothermia-pretreatment-protects-against-liver-ischemia-reperfusion-injury-via-the-pi3k-akt-foxo3a-pathway
#13
Qi Xiao, Qifa Ye, Wei Wang, Jiansheng Xiao, Biqi Fu, Zhiping Xia, Xingjian Zhang, Zhongzhong Liu, Xianpeng Zeng
Mild hypothermia is known to protect against ischemia and reperfusion (IR) injury. The exact mechanisms of the protection are not fully understood. Forkhead box O3 (FOXO3a) has been defined as a critical mediator in cellular processes, including oxidative stress, apoptosis, inflammation, cell death and DNA repair; however, the protection function in mild hypothermia has not been reported previously. The current study was designed to investigate the function of phosphoinositide 3‑kinase (PI3K)/protein kinase B (AKT)/FOXO3a pathway in pretreatment with mild hypothermia during IR injury...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944805/stretchable-3d-lattice-conductors
#14
Tingyao Li, Yanhui Jiang, Kunhao Yu, Qiming Wang
3D architectures have been long harnessed to create lightweight yet strong cellular materials; however, the study regarding how 3D architectures facilitate the design of soft materials is at the incipient stage. Here, we demonstrate that 3D architectures can greatly facilitate the design of an intrinsically stretchable lattice conductor. We show that 3D architectures can be harnessed to enhance the overall stretchability of the soft conductors, reduce the effective density, enable resistive sensing of the large deformation of curved solids, and improve monitoring of a wastewater stream...
September 25, 2017: Soft Matter
https://www.readbyqxmd.com/read/28944802/subcellular-compartmentalisation-of-copper-iron-manganese-and-zinc-in-the-parkinson-s-disease-brain
#15
Sian Genoud, Blaine R Roberts, Adam P Gunn, Glenda M Halliday, Simon J G Lewis, Helen J Ball, Dominic J Hare, Kay L Double
Elevated iron and decreased copper levels are cardinal features of the degenerating substantia nigra pars compacta in the Parkinson's disease brain. Both of these redox-active metals, and fellow transition metals manganese and zinc, are found at high concentrations within the midbrain and participate in a range of unique biological reactions. We examined the total metal content and cellular compartmentalisation of manganese, iron, copper and zinc in the degenerating substantia nigra, disease-affected but non-degenerating fusiform gyrus, and unaffected occipital cortex in the post mortem Parkinson's disease brain compared with age-matched controls...
September 25, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28944771/the-spectrum-of-t-cell-metabolism-in-health-and-disease
#16
REVIEW
Glenn R Bantug, Lorenzo Galluzzi, Guido Kroemer, Christoph Hess
In healthy individuals, metabolically quiescent T cells survey lymph nodes and peripheral tissues in search of cognate antigens. During infection, T cells that encounter cognate antigens are activated and - in a context-specific manner - proliferate and/or differentiate to become effector T cells. This process is accompanied by important changes in cellular metabolism (known as metabolic reprogramming). The magnitude and spectrum of metabolic reprogramming as it occurs in T cells in the context of acute infection ensure host survival...
September 25, 2017: Nature Reviews. Immunology
https://www.readbyqxmd.com/read/28944765/folate-receptor-targeted-nir-sensitive-polydopamine-nanoparticles-for-chemo-photothermal-cancer-therapy
#17
Hao Li, Zhen Jin, Sunghoon Cho, Mi Jeong Jeon, Van Du Nguyen, Jong-Oh Park, Sukho Park
We propose the use of folate-receptor-targeted, near-infrared-sensitive polydopamine nanoparticles (NPs) for chemo-photothermal cancer therapy as an enhanced type of drug-delivery system which can be synthesized by in situ polymerization and conjugation with folic acid. The NPs consist of a Fe3O4/Au core, coated polydopamine, conjugated folic acid, and loaded anti-cancer drug (doxorubicin). The proposed multifunctional NPs show many advantages for therapeutic applications such as good biocompatibility and easy bioconjugation...
September 25, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28944749/keeping-communications-flowing-during-large-scale-disasters-leveraging-amateur-radio-innovations-for-disaster-medicine
#18
Victor H Cid, Andrew R Mitz, Stacey J Arnesen
Medical facilities may struggle to maintain effective communications during a major disaster. Natural and man-made disasters threaten connectivity by degrading or crippling Internet, cellular/mobile, and landline telephone services across wide areas. Communications among staff, between facilities, and to resources outside the disaster area may be lost for an extended time. A prototype communications system created by the National Library of Medicine (NLM) provides basic communication services that ensure essential connectivity in the face of widespread infrastructure loss...
September 25, 2017: Disaster Medicine and Public Health Preparedness
https://www.readbyqxmd.com/read/28944743/alginate-combined-cholic-acid-increased-insulin-secretion-of-microencapsulated-mouse-cloned-pancreatic-%C3%AE-cells
#19
Armin Mooranian, Rebecca Negrulj, Ryu Takechi, Emma Jamieson, Grant Morahan, Hani Al-Salami
AIM: A semisynthetic primary bile acid (PBA) has exerted hypoglycemic effects in Type 1 diabetic animals, which were hypothesized to be due to its anti-inflammatory and cellular glucose-regulatory effects. Thus, the research purpose aimed to examine antidiabetic effects of a PBA, in terms of cellular inflammation and survival and insulin release, in the context of supporting β-cell delivery and Type 1 diabetic treatment. MATERIALS & METHODS: 10 formulations were prepared, five without PBA (control) and five with PBA (test)...
October 2017: Therapeutic Delivery
https://www.readbyqxmd.com/read/28944735/strategies-in-the-design-of-gold-nanoparticles-for-intracellular-targeting-opportunities-and-challenges
#20
Yamin Yang, Lei Ren, Hongjun Wang
With unique physicochemical properties, gold nanoparticles (Au NPs) have demonstrated their potential as drug carriers or therapeutic agents. Effective guidance of Au NPs into specific intracellular destinations becomes increasingly important as we strive to further improve the efficiency of drug delivery and modulate controllable cellular responses. In this review, we summarized recent advances in designing Au NPs with the capabilities of cellular penetration and internalization, endosomal escape, intracellular trafficking and subcellular localization via various approaches including physical injection, tuning the physiochemical parameters of Au NPs, and surface modification with targeting ligands...
October 2017: Therapeutic Delivery
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