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https://www.readbyqxmd.com/read/28731322/large-scale-stretchable-semi-embedded-copper-nanowires-transparent-conductive-films-by-electrospinning-template
#1
Xia Yang, Xiaotian Hu, Qingxia Wang, Jian Xiong, Hanjun Yang, Xiangchuan Meng, Licheng Tan, Lie Chen, Yiwang Chen
With recent emergence of wearable electronic devices, the flexible and stretchable transparent electrodes are the core components to realize innovative devices. Copper nanowires (CuNWs) network is commonly chosen because of its high conductivity and transparency. However, the junction resistances and low aspect ratios still limit its further stretchable performance. Herein, a large-scale stretchable semi-embedded CuNWs transparent conductive film (TCFs) was fabricated by electrolessly depositing Cu on the electrospun poly (4-vinylpyridine) (P4VP) polymer template semi-embedded in polydimethylsiloxane (PDMS)...
July 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28730250/metastatic-high-grade-myxofibrosarcoma-review-of-a-clinical-case
#2
Tiberiu Paul Neagu, Ruxandra Diana Sinescu, Valentin Enache, Sanda Claudia Achim, Mirela Ţigliş, Liliana Elena Mirea
We present the particular clinical and histological features of a metastatic high-grade myxofibrosarcoma (MFS) of the left buttock in a 77-year-old male patient. The tumor was biopsied and surgically removed in order to increase the patient's comfort, due to its increased size and aggressive clinical behavior. Computed tomography (CT) revealed metastases in the pleura and mediastinal lymph nodes, so limb-sparing tumor excision followed by palliative care was the best practice for the patient until the fatal outcome...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28721884/dosimetric-predictors-of-patient-reported-xerostomia-and-dysphagia-with-deintensified-chemoradiation-therapy-for-hpv-associated-oropharyngeal-squamous-cell-carcinoma
#3
Bhishamjit S Chera, David Fried, Alex Price, Robert J Amdur, William Mendenhall, Chiray Lu, Shiva Das, Nathan Sheets, Lawrence Marks, Panayiotis Mavroidis
PURPOSE/OBJECTIVE(S): To estimate the association between different dose-volume metrics of the salivary glands and pharyngeal constrictors with patient reported severity of xerostomia/dysphagia in the setting of deintensified chemoradiation therapy (CRT). METHODS AND MATERIALS: Forty-five patients were treated on a phase 2 study assessing the efficacy of deintensified CRT for favorable-risk, HPV-associated oropharyngeal squamous cell carcinoma. Patients received 60 Gy intensity modulated radiation therapy with concurrent weekly cisplatin (30 mg/m(2)), and reported the severity of their xerostomia/dysphagia (before and after treatment) using the patient-reported outcome version of the Common Terminology Criteria for Adverse Events (CTCAE) (PRO-CTCAE)...
August 1, 2017: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/28719734/tissue-engineering-of-a-composite-trachea-construct-using-autologous-rabbit-chondrocytes
#4
James E Dennis, Kristina G Bernardi, Thomas J Kean, Nelson E Liou, Tanya K Meyer
The repair of large tracheal segmental defects remains an unsolved problem. The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements. Engineered tracheal replacements composed of autologous cells ("neotracheas") were tested in a New Zealand White rabbit model. Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle and an engineered cartilage sheet, and allowing the construct to mature for 8-12 weeks...
July 18, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28719466/dedifferentiated-liposarcoma-with-epithelioid-epithelial-features
#5
Naohiro Makise, Akihiko Yoshida, Motokiyo Komiyama, Fumihiko Nakatani, Kan Yonemori, Akira Kawai, Masashi Fukayama, Nobuyoshi Hiraoka
Dedifferentiated liposarcoma (DDLPS) demonstrates a variety of growth patterns, and their histologic resemblance to other spindle cell mesenchymal tumors has been widely recognized. However, epithelioid morphology in DDLPS has only rarely been documented. Here, we report 6 cases of DDLPS with striking epithelioid/epithelial features. The patients were 5 men and 1 woman with a median age of 61 years. All tumors were located in the internal trunk. During follow-up of 1 to 41 months, local recurrence, distant metastases, and tumor-related death occurred in 4, 2, and 4 patients, respectively...
July 20, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28718622/graphene-sheet-induced-global-maturation-of-cardiomyocytes-derived-from-human-induced-pluripotent-stem-cells
#6
Jiaxian Wang, Chang Cui, Haiyan Nan, Yuanfang Yu, Yini Xiao, Ellen Poon, Gang Yang, Xijie Wang, Chenchen Wang, Lingsong Li, Kenneth Richard Boheler, Xu Ma, Xin Cheng, Zhenhua Ni, Minglong Chen
Human induced pluripotent stem cells (hiPSCs) can proliferate infinitely. Their ability to differentiate into cardiomyocytes provides abundant sources for disease modeling, drug screening and regenerative medicine. However, hiPSC-derived cardiomyocytes (hiPSC-CMs) display a low degree of maturation and fetal-like properties. Current in vitro differentiation methods do not mimic the structural, mechanical, and physiological properties of the cardiogenesis niche. Recently, we present an efficient cardiac maturation platform that combines hiPSCs monolayer cardiac differentiation with graphene substrate which is a biocompatible and superconductive material...
July 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28714276/human-platelet-lysate-supports-the-formation-of-robust-human-periodontal-ligament-cell-sheets
#7
Bei-Min Tian, Rui-Xin Wu, Chun-Sheng Bi, Xiao-Tao He, Yuan Yin, Fa-Ming Chen
The use of stem cell-derived sheets has become increasingly common in a wide variety of biomedical applications. Although substantial evidence has demonstrated that human platelet lysate (PL) can be used for therapeutic cell expansion, either as a substitute for or as a supplement to xenogeneic fetal bovine serum (FBS), its impact on cell sheet production remains largely unexplored. In this study, we manufactured periodontal ligament stem cell (PDLSC) sheets in vitro by incubating PDLSCs in sheet-induction media supplemented with various ratios of PL and FBS, i...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714246/carbon-nanodot-sensitized-modulation-of-alzheimer-s-%C3%AE-amyloid-self-assembly-disassembly-and-toxicity
#8
You Jung Chung, Kayoung Kim, Byung Il Lee, Chan Beum Park
The self-assembly of amyloidogenic peptides into β-sheet-rich aggregates is a general feature of many neurodegenerative diseases, including Alzheimer's disease, which signifies the need for the effective attenuation of amyloid aggregation toward alleviating amyloid-associated neurotoxicity. This study reports that photoluminescent carbon nanodots (CDs) can effectively suppress Alzheimer's β-amyloid (Aβ) self-assembly and function as a β-sheet breaker disintegrating preformed Aβ aggregates. This study synthesizes CDs using ammonium citrate through one-pot hydrothermal treatment and passivates their surface with branched polyethylenimine (bPEI)...
July 17, 2017: Small
https://www.readbyqxmd.com/read/28714140/prevascularization-of-natural-nanofibrous-extracellular-matrix-for-engineering-completely-biological-3d-prevascularized-tissues-for-diverse-applications
#9
Lijun Zhang, Zichen Qian, Mitchell Tahtinen, Shaohai Qi, Feng Zhao
Self-sustainability after implantation is one of the critical obstacles facing large engineered tissues. A preformed functional vascular network provides an effective solution for solving the mass transportation problem. With the support of mural cells, endothelial cells (ECs) can form microvessels within engineered tissues. As an important mural cell, human mesenchymal stem cells (hMSCs) not only stabilize the engineered microvessel network, but also preserve their multi-potency when grown under optimal culture conditions...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28713709/high-quality-of-infant-chondrocytes-in-comparison-with-adult-chondrocytes-for-cartilage-tissue-engineering
#10
Fatemeh Mortazavi, Hajar Shafaei, Jafar Soleimani Rad, Leila Rushangar, Azadeh Montaceri, Masoud Jamshidi
BACKGROUND: Tissue engineering is used for the treatment of many diseases, and the ideal cell source for cartilage tissue engineering is chondrocytes. The main limitation of chondrocyte is the low number of cells in cartilage tissue engineering. This study investigated a suitable cell source with high proliferation rate to obtain a large number of chondrocytes. METHODS: Adult cartilage tissue samples were obtained from adult patients undergoing surgical procedure, and infant cartilage tissue samples were obtained from polydactyly surgical waste...
May 2017: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/28713638/neurovascular-cell-sheet-transplantation-in-a-canine-model-of-intracranial-hemorrhage
#11
Woo-Jin Lee, Jong Young Lee, Keun-Hwa Jung, Soon-Tae Lee, Hyo Yeol Kim, Dong-Kyu Park, Jung-Suk Yu, So-Yun Kim, Daejong Jeon, Manho Kim, Sang Kun Lee, Jae-Kyu Roh, Kon Chu
Cell-based therapy for intracerebral hemorrhage (ICH) has a great therapeutic potential. However, methods to effectively induce direct regeneration of the damaged neural tissue after cell transplantation have not been established, which, if done, would improve the efficacy of cell-based therapy. In this study, we aimed to develop a cell sheet with neurovasculogenic potential and evaluate its usefulness in a canine ICH model. We designed a composite cell sheet made of neural progenitors derived from human olfactory neuroepithelium and vascular progenitors from human adipose tissue-derived stromal cells...
2017: Cell Medicine
https://www.readbyqxmd.com/read/28713066/approaches-for-stricture-prevention-after-esophageal-endoscopic-resection
#12
REVIEW
Seiichiro Abe, Prasad G Iyer, Ichiro Oda, Nobuo Kanai, Yutaka Saito
BACKGROUND AND AIMS: Endoscopic resection of extensive esophageal lesions has become more common as endoscopic resection techniques and equipment has developed. However, extensive esophageal endoscopic resections can cause postoperative esophageal strictures, which have a negative impact on the quality of life of patients. We aimed to review current treatments and innovative approaches to prevent esophageal strictures after widespread endoscopic resection of esophageal lesions. METHODS: We performed a comprehensive literature search using predetermined search terms from 2000 to 2016 to identify relevant articles and summarized their results as a narrative review...
July 13, 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/28712875/apical-constriction-in-distal-visceral-endoderm-cells-initiates-global-collective-cell-rearrangement-in-embryonic-visceral-endoderm-to-form-anterior-visceral-endoderm
#13
Go Shioi, Hideharu Hoshino, Takaya Abe, Hiroshi Kiyonari, Kazuki Nakao, Wenxiang Meng, Yasuhide Furuta, Toshihiko Fujimori, Shinichi Aizawa
The behavior of visceral endoderm cells was examined as the anterior visceral endoderm (AVE) formed from the distal visceral endoderm (DVE) using the mouse lines R26-H2B-EGFP and R26-PHA7-EGFP to visualize cell nuclei and adherens junction, respectively. The analysis using R26-H2B-EGFP demonstrated global cell rearrangement that was not specific to the DVE cells in the monolayer embryonic visceral endoderm sheet; each population of the endoderm cells moved collectively in a swirling movement as a whole. Most of the AVE cells at E6...
July 13, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28711240/characterization-and-bioactivities-of-the-exopolysaccharide-from-a-probiotic-strain-of-lactobacillus-plantarum-wlpl04
#14
Zhengqi Liu, Zhihong Zhang, Liang Qiu, Fen Zhang, Xiongpeng Xu, Hua Wei, Xueying Tao
Exopolysaccharide (EPS) was extracted and purified from Lactobacillus plantarum WLPL04, which has been confirmed previously as a potential probiotic for its antagonistic and immune-modulating activity. It has a molecular weight of 6.61 × 10(4) Da, consisting of xylose, glucose, and galactose in an approximate molar ratio of 3.4: 1.8: 1. Microstructural studies demonstrated that the EPS appeared as a smooth sheet structure with many homogeneous rod-shaped lumps. The preliminary in vitro assays indicated that the EPS could significantly inhibit the adhesion of Escherichia coli O157:H7 to HT-29 cells in competition, replacement, and inhibition assays at a dose of 1...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28710569/low-shear-modeled-microgravity-grown-penicillium-chrysogenum-mediated-biosynthesis-of-silver-nanoparticles-with-enhanced-antimicrobial-activity-and-its-anticancer-effect-in-human-liver-cancer-and-fibroblast-cells
#15
Sunirmal Sheet, Yesupatham Sathishkumar, Allur Subramaniyam Sivakumar, Kwan Seob Shim, Yang Soo Lee
Gravitational force and shear forces induce various changes in gene expression and metabolite production of microorganisms. Previous reports have shown that there are differences in the expression of different sets of proteins and enzymes under microgravity conditions compared to normal gravity. The aim of this study is to utilize culture filtrates of Penicillium chrysogenum grown under microgravity and normal conditions to synthesize silver nanoparticles and to examine whether there is any difference between their physiochemical and biological function...
July 14, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28710268/physiological-evidence-for-isopotential-tunneling-in-the-electron-transport-chain-of-methane-producing-archaea
#16
Nikolas Duszenko, Nicole R Buan
Many, but not all, organisms use quinones to conserve energy in their electron transport chains. Fermentative bacteria and methane-producing archaea (methanogens) do not produce quinones but have devised other ways to generate ATP. Methanophenazine (MPh) is a unique membrane electron carrier found in Methanosarcina species that plays the same role as quinones in the electron transport chain. To extend the analogy between quinones and MPh, we compared the MPh pool sizes between two well-studied Methanosarcina species, Methanosarcina acetivorans C2A and Methanosarcina barkeri Fusaro to the quinone pools sizes in the bacterium, Escherichia coli We found the quantity of MPh per cell increases as cultures transition from exponential growth to stationary phase, and absolute quantities of MPh were 3-fold higher in M...
July 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28709984/micrometer-scale-guidance-of-mesenchymal-stem-cells-to-form-structurally-oriented-large-scale-tissue-engineered-cartilage
#17
Chih-Ling Chou, Alexander L Rivera, Valencia Williams, Jean F Welter, Joseph M Mansour, Judith A Drazba, Takao Sakai, Harihara Baskaran
Current clinical methods to treat articular cartilage lesions provide temporary relief of the symptoms but fail to permanently restore the damaged tissue. Tissue engineering, using mesenchymal stem cells (MSCs) combined with scaffolds and bioactive factors, is viewed as a promising method for repairing cartilage injuries. However, current tissue engineered constructs display inferior mechanical properties compared to native articular cartilage, which could be attributed to the lack of structural organization of the extracellular matrix (ECM) of these engineered constructs in comparison to the highly oriented structure of articular cartilage ECM...
July 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28707336/mechanical-role-of-the-spatial-patterns-of-contractile-cells-in-invagination-of-growing-epithelial-tissue
#18
Yasuhiro Inoue, Tadashi Watanabe, Satoru Okuda, Taiji Adachi
Epithelial invagination is one of the fundamental deformation modes during morphogenesis, and is essential for deriving the three-dimensional shapes of organs from a flat epithelial sheet. Invagination occurs in an orderly manner according to the spatial pattern of the contractile cells; however, it remains elusive how tissue deformation can be caused by cellular activity in the patterned region. In this study, we investigated the mechanical role of the spatial patterns of the contractile cells in invagination of growing tissue using multicellular dynamics simulations...
July 13, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28706272/modeling-atrial-fibrillation-using-human-embryonic-stem-cell-derived-atrial-tissue
#19
Zachary Laksman, Marianne Wauchop, Eric Lin, Stephanie Protze, Jeehoon Lee, Wallace Yang, Farzad Izaddoustdar, Sanam Shafaattalab, Lior Gepstein, Glen F Tibbits, Gordon Keller, Peter H Backx
Since current experimental models of Atrial Fibrillation (AF) have significant limitations, we used human embryonic stem cells (hESCs) to generate an atrial-specific tissue model of AF for pharmacologic testing. We generated atrial-like cardiomyocytes (CMs) from hESCs which preferentially expressed atrial-specific genes, and had shorter action potential (AP) durations compared to ventricular-like CMs. We then generated confluent atrial-like CM sheets and interrogated them using optical mapping techniques. Atrial-like CM sheets (~1 cm in diameter) showed uniform AP propagation, and rapid re-entrant rotor patterns, as seen in AF could be induced...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28705807/augmentation-of-cgmp-pkg-pathway-and-colonic-motility-by-hydrogen-sulfide
#20
Ancy D Nalli, Sayak Bhattacharya, Hongxia Wang, Derek M Kendig, John R Grider, Karnam S Murthy
Hydrogen sulfide (H2S), like nitric oxide (NO), causes smooth muscle relaxation, but unlike NO, does not stimulate soluble guanylyl cyclase (sGC) activity and generate cGMP. The aim of this study was to investigate the interplay between NO and H2S in colonic smooth muscle. In colonic smooth muscle from rabbit, mouse and human, L-cysteine, substrate of cystathionine-γ-lyase (CSE), or NaHS, an H2S donor, inhibited phosphodiesterase 5 (PDE5) activity and augmented the increase in cGMP levels, IP3 receptor phosphorylation at Ser(1756) (measured as a proxy for PKG activation), and muscle relaxation in response to NO donor, s-nitrosoglutathione (GSNO), suggesting augmentation of cGMP/PKG pathway by H2S...
July 13, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
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