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https://www.readbyqxmd.com/read/28432357/in-silico-drug-discovery-approach-targeting-receptor-tyrosine-kinase-like-orphan-receptor-1-for-cancer-treatment
#1
Onkar Nath, Archana Singh, Indrakant K Singh
Receptor tyrosine kinases (RTK) are important cell signaling molecules that influence many cellular processes. Receptor tyrosine kinase such as orphan receptor 1 (Ror1), a surface antigen, is a member of the RTK family of Ror, which plays a crucial role in cancers that have high-grade histology. As Ror1 has been implicated to be a potential target for cancer therapy, we selected this protein for further investigation. The secondary and tertiary structure of this protein was determined, which revealed that this protein contained three β-sheets, seven α-helices, and coils...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432219/optimized-cubic-protocol-for-3d-imaging-of-chicken-embryos-at-single-cell-resolution
#2
María Victoria Gómez-Gaviro, Evan Balaban, Diana Bocancea, María Teresa Lorrio, María Pompeiano, Manuel Desco, Jorge Ripoll, Juan José Vaquero
The CUBIC tissue clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell-level resolution in whole early-stage chicken embryos, and in the whole brains of late-stage embryos.
April 21, 2017: Development
https://www.readbyqxmd.com/read/28432017/dual-functional-nanoparticles-for-precise-drug-delivery-to-alzheimer-s-disease-lesions-targeting-mechanisms-pharmacodynamics-and-safety
#3
Xiaoyao Zheng, Chi Zhang, Qian Guo, Xu Wan, Xiayan Shao, Qingfeng Liu, Qizhi Zhang
Alzheimer's disease (AD) is the most common form of dementia and is characterized by the cerebral accumulation of extracellular amyloid plaques. In a previous study, this histopathological hallmark was used as a target on a dual-functional nanoparticle (TQNP) to deliver biotechnological drugs, such as the H102 peptide, a β-sheet breaker, to AD lesions precisely. This delivery system could reduce the amyloid-β (Aβ) burden in the brains of AD model mice, as well as ameliorated the memory impairment of the mice...
April 18, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28431400/local-delivery-of-hmgb1-in-gelatin-sponge-scaffolds-combined-with-mesenchymal-stem-cell-sheets-to-accelerate-fracture-healing
#4
Deting Xue, Wei Zhang, Erman Chen, Xiang Gao, Ling Liu, Chenyi Ye, Yanbin Tan, Zhijun Pan, Hang Li
Fracture nonunion and delayed union continue to pose challenges for orthopedic surgeons. In the present study, we combined HMGB1 gelatin sponges with MSC sheets to promote bone healing after surgical treatment of rat tibial fractures. The HMGB1 gelatin sponge scaffolds supported the expansion of mesenchymal stem cells (MSCs) and promoted the osteogenic differentiation of MSCs and MSC sheets. Lentiviral vectors were then used to overexpress HMGB1 in MSCs. The results indicated that HMGB1 promotes the osteogenic differentiation of MSCs through the STAT3 pathway...
April 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28424631/mechanisms-underlying-the-emergence-of-post-acidosis-arrhythmia-at-the-tissue-level-a-theoretical-study
#5
Jieyun Bai, Renli Yin, Kuanquan Wang, Henggui Zhang
Acidosis has complex electrophysiological effects, which are associated with a high recurrence of ventricular arrhythmias. Through multi-scale cardiac computer modeling, this study investigated the mechanisms underlying the emergence of post-acidosis arrhythmia at the tissue level. In simulations, ten Tusscher-Panfilov ventricular model was modified to incorporate various data on acidosis-induced alterations of cellular electrophysiology and intercellular electrical coupling. The single cell models were incorporated into multicellular one-dimensional (1D) fiber and 2D sheet tissue models...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28423686/clinical-features-and-prognosis-for-intraventricular-liponeurocytoma
#6
Nini Xu, Jinxiu Cai, Jiang Du, Rong Yang, Huachen Zhu, Peiyi Gao, Jian Zhou, Xiaofeng Li
Cerebellar liponeurocytoma is a rare central nervous system tumor, we investigate its biological behaviors and clinical prognosis to improve the understanding of this tumor. We retrospectively analyzed the clinical, radiological and histopathological findings as well as follow-up data of two patients with intraventricular liponeurocytomas in Beijing Tiantan Hospital between July 2000 and July 2016. The main clinical manifestations of the two patients were headache. The supratentorial intraventricular liponeurocytoma appeared as isodense to slight hyperdense on CT scan and heterogeneous intensity on T1-weighted imaging (T1WI) and T2-weighted imaging (T2WI)...
March 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423004/variations-in-the-interaction-of-human-defensins-with-escherichia-coli-possible-implications-in-bacterial-killing
#7
Basil Mathew, Ramakrishnan Nagaraj
Human α and β-defensins are cationic antimicrobial peptides characterized by three disulfide bonds with a triple stranded β-sheet motif. It is presumed that interaction with the bacterial cell surface and membrane permeabilization by defensins is an important step in the killing process. In this study, we have compared interactions of three human α-defensins HNP3, HNP4, HD5 and human β-defensins HBD1-4 that are active against Escherichia coli, with its cell surface and inner membrane as well as negatively charged model membranes...
2017: PloS One
https://www.readbyqxmd.com/read/28422570/an-induced-adipocyte-sheet-reduces-inflammatory-reactions-during-remodeling-of-xenogeneic-scaffolds-in-vivo
#8
Tong Yi, Qiang Wang, Fan Fan, Gouhui Fan
Surgical therapy of cardiovascular diseases frequently requires replacement of diseased tissues with prosthetic devices or grafts. Calcification is the main reason for the degeneration of implanted grafts. However, some factors reduce stenosis and attenuate calcification of implanted grafts. In this study, we used an autologous induced adipocyte cell-sheet (IACS) as a drug delivery system to determine whether its secretion ability has a beneficial effect on the remodeling process of grafts in a rat subcutaneous model...
April 19, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28418636/defining-molecular-details-of-the-chemistry-of-biofilm-formation-by-raman-microspectroscopy
#9
Paul R Carey, Blake R Gibson, Jordan F Gibson, Michael E Greenberg, Hossein Heidari-Torkabadi, Marianne Pusztai-Carey, Sean T Weaver, Grant R Whitmer
Two protocols that allow for the comparison of Raman spectra of planktonic cells and biofilm formed from these cells in their growth phase have been developed. Planktonic cells are washed and flash-frozen in <1 min to reduce the time for metabolic changes during processing, prior to freeze-drying. Biofilm is formed by standing cells in 50 μL indentations in aluminum foil in an atmosphere of saturated water vapor for 24-48 h. The results for Escherichia coli type K12 cells, which do not readily form biofilm, are compared to those for Staphylococcus epidermidis cells, which prolifically synthesize biofilm...
April 19, 2017: Biochemistry
https://www.readbyqxmd.com/read/28416163/nanosheets-for-delivery-of-biomolecules-into-plant-cells
#10
Wenlong Bao, Yinglang Wan, František Baluška
Layered double hydroxides (LDHs) are sheet-formed nanoparticles (NPs) of adjustable size. It has recently been reported that LDHs have the ability to deliver biomolecules into intact plant cells. LDHs show promise as a novel and powerful tool for plant cell studies and similar applications.
April 13, 2017: Trends in Plant Science
https://www.readbyqxmd.com/read/28416106/categorizing-renal-oncocytic-neoplasms-on-core-needle-biopsy-a-morphologic-and-immunophenotypic-study-of-144-cases-with-clinical-follow-up-alderman-ma-daignault-s-wolf-js-jr-palapattu-gs-weizer-az-hafez-ks-kunju-lp-wu-aj-hum-pathol-september-2016-55-1-10
#11
Oleksandr N Kryvenko
There is limited literature on renal oncocytic neoplasms diagnosed on core biopsy. All renal oncocytic neoplasm core biopsies from 2006 to 2013 were, retrospectively, reviewed. Morphologic features and an immunohistochemical panel of CK7, c-KIT, and S100A1 were assessed. Concordance with resection diagnosis, statistical analysis including a random forest classification, and follow-up were recorded. The postimmunohistochemical diagnoses of 144 renal oncocytic core biopsies were favor oncocytoma (67%), favor renal cell carcinoma (RCC) (12%), and cannot exclude RCC (21%)...
April 13, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28415270/pinning-of-scroll-waves-to-flat-and-highly-branched-unexcitable-heterogeneities
#12
Dhriti Mahanta, Sumana Dutta, Oliver Steinbock
System heterogeneities such as organelles, cells, and anatomical features strongly affect nonlinear wave patterns in biological systems. These effects are more readily studied in otherwise homogeneous chemical reactions that allow the introduction of tailored structures. Following this approach, we investigate the dynamics of three-dimensional excitation vortices pinned to inert sheets with circular holes arranged on a hexagonal lattice. Experiments with the Belousov-Zhabotinsky reaction and numerical simulations of an excitable reaction-diffusion model reveal vortex pinning that circumvents the rapid collapse of free vortex rings...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28415157/a-small-case-series-of-intravascular-large-b-cell-lymphoma-with-unexpected-findings-subset-of-cases-with-concomitant-extravascular-central-nervous-system-cns-involvement-mimicking-primary-cns-lymphoma
#13
Kate Poropatich, Dave Dittmann, Yi-Hua Chen, Kirtee Raparia, Kristy Wolniak, Juehua Gao
Background: Intravascular large B-cell lymphoma (IVLBCL) is a rare type of extranodal lymphoma with growth mainly in the lumina of vessels. We studied a small series of IVLBCL and focused on its central nervous system (CNS) involvement. Materials and Methods: Searching the medical records of Northwestern Memorial Hospital, we identified five cases of IVLBCL from January 2007 to January 2015. Clinical information, hematoxylin and eosin stained histologic slides and immunohistochemistry studies were reviewed for all cases...
April 17, 2017: Journal of Pathology and Translational Medicine
https://www.readbyqxmd.com/read/28414372/graphene-based-near-field-optical-microscopy-high-resolution-imaging-using-reconfigurable-gratings
#14
Sandeep Inampudi, Jierong Cheng, Hossein Mosallaei
High-resolution and fast-paced optical microscopy is a requirement for current trends in biotechnology and materials industry. The most reliable and adaptable technique so far to obtain higher resolution than conventional microscopy is near-field scanning optical microscopy (NSOM), which suffers from a slow-paced nature. Stemming from the principles of diffraction imaging, we present fast-paced graphene-based scanning-free wide-field optical microscopy that provides image resolution that competes with NSOM...
April 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/28414273/oriented-clonal-cell-dynamics-enables-accurate-growth-and-shaping-of-vertebrate-cartilage
#15
Marketa Kaucka, Tomas Zikmund, Marketa Tesarova, Daniel Gyllborg, Andreas Hellander, Josef Jaros, Jozef Kaiser, Julian Petersen, Bara Szarowska, Phillip T Newton, Vyacheslav Dyachuk, Lei Li, Hong Qian, Anne-Sofie Johansson, Yuji Mishina, Josh Currie, Elly M Tanaka, Alek Erickson, Andrew Dudley, Hjalmar Brismar, Paul Southam, Enrico Coen, Min Chen, Lee S Weinstein, Ales Hampl, Ernest Arenas, Andrei S Chagin, Kaj Fried, Igor Adameyko
Cartilaginous structures are at the core of embryo growth and shaping before the bone forms. Here we report a novel principle of vertebrate cartilage growth that is based on introducing transversally-oriented clones into pre-existing cartilage. This mechanism of growth uncouples the lateral expansion of curved cartilaginous sheets from the control of cartilage thickness, a process which might be the evolutionary mechanism underlying adaptations of facial shape. In rod-shaped cartilage structures (Meckel, ribs and skeletal elements in developing limbs), the transverse integration of clonal columns determines the well-defined diameter and resulting rod-like morphology...
April 17, 2017: ELife
https://www.readbyqxmd.com/read/28412975/effect-of-cryopreservation-on-proliferation-and-differentiation-of-periodontal-ligament-stem-cell-sheets
#16
Mengying Li, Cheng Feng, Xiuge Gu, Qin He, Fulan Wei
BACKGROUND: Cryopreservation has been extensively applied to the long-term storage of a diverse range of biological materials. However, no comprehensive study is currently available on the cryopreservation of periodontal ligament stem cell (PDLSC) sheets which have been suggested as excellent transplant materials for periodontal tissue regeneration. The aim of this study is to investigate the effect of cryopreservation on the structural integrity and functional viability of PDLSC sheets...
April 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28412555/one-dimensional-poly-l-lysine-block-poly-l-threonine-assemblies-exhibit-potent-anticancer-activity-by-enhancing-membranolysis
#17
Yu-Fon Chen, Ai-Li Shiau, Sue-Joan Chang, Nai-Shin Fan, Chung-Teng Wang, Chao-Liang Wu, Jeng-Shiung Jan
Herein, we report the oncolytic activity of cationic, one-dimensional (1D) fibril assemblies formed from coil-sheet poly(L-lysine)-block-poly(L-threonine) (PLL-b-PLT) block copolypeptides for cancer therapy. The 1D fibril assemblies can efficiently interact with negatively charged cellular and mitochondrial membranes via electrostatic interactions, leading to necrosis via membrane lysis and apoptosis via the mitochondria-lytic effect. The concept is analogous to that of 1D drug carriers that exhibit enhanced cell penetration...
April 12, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28410407/bacterial-amyloid-curli-acts-as-a-carrier-for-dna-to-elicit-an-autoimmune-response-via-tlr2-and-tlr9
#18
Sarah A Tursi, Ernest Y Lee, Nicole J Medeiros, Michael H Lee, Lauren K Nicastro, Bettina Buttaro, Stefania Gallucci, Ronald Paul Wilson, Gerard C L Wong, Çagla Tükel
Bacterial biofilms are associated with numerous human infections. The predominant protein expressed in enteric biofilms is the amyloid curli, which forms highly immunogenic complexes with DNA. Infection with curli-expressing bacteria or systemic exposure to purified curli-DNA complexes triggers autoimmunity via the generation of type I interferons (IFNs) and anti-double-stranded DNA antibodies. Here, we show that DNA complexed with amyloid curli powerfully stimulates Toll-like receptor 9 (TLR9) through a two-step mechanism...
April 14, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28408991/transplantation-of-tissue-engineered-cell-sheets-for-stricture-prevention-after-endoscopic-submucosal-dissection-of-the-oesophagus
#19
Eduard Jonas, Sebastian Sjöqvist, Peter Elbe, Nobuo Kanai, Jenny Enger, Stephan L Haas, Ammar Mohkles-Barakat, Teruo Okano, Ryo Takagi, Takeshi Ohki, Masakazu Yamamoto, Makoto Kondo, Katrin Markland, Mei Ling Lim, Masayuki Yamato, Magnus Nilsson, Johan Permert, Pontus Blomberg, J-Matthias Löhr
BACKGROUND AND OBJECTIVE: Endoscopic mucosal dissection (ESD) is a treatment option for oesophagus tumours localized to the mucosa enabling en bloc removal of large lesions. The resulting larger mucosal defects have resulted in an increase in the occurrence of post-treatment strictures. Transplantation of autologous cell sheets, cultured from oral mucosa, has been shown to prevent post-ESD strictures. The aim of the study was to assess the efficacy and safety of cell sheet transplantation after oesophageal ESD in a Western patient population where reflux-associated pre-malignant and malignant conditions predominate...
December 2016: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28404971/association-between-tumor-architecture-derived-from-generalized-q-space-mri-and-survival-in-glioblastoma
#20
Erik N Taylor, Yao Ding, Shan Zhu, Eric Cheah, Phillip Alexander, Leon Lin, George E Aninwene Ii, Matthew P Hoffman, Anita Mahajan, Abdallah S R Mohamed, Nathan McDannold, Clifton D Fuller, Clark C Chen, Richard J Gilbert
While it is recognized that the overall resistance of glioblastoma to treatment may be related to intra-tumor patterns of structural heterogeneity, imaging methods to assess such patterns remain rudimentary. METHODS: We utilized a generalized Q-space imaging (GQI) algorithm to analyze magnetic resonance imaging (MRI) derived from a rodent model of glioblastoma and 2 clinical datasets to correlate GQI, histology, and survival. RESULTS: In a rodent glioblastoma model, GQI demonstrated a poorly coherent core region, consisting of diffusion tracts <5 mm, surrounded by a shell of highly coherent diffusion tracts, 6-25 mm...
March 16, 2017: Oncotarget
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