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https://www.readbyqxmd.com/read/29352711/gold-cleaning-methods-for-preparation-of-cell-culture-surfaces-for-self-assembled-monolayers-of-zwitterionic-oligopeptides
#1
Junko Enomoto, Tatsuto Kageyama, Dina Myasnikova, Kisaki Onishi, Yuka Kobayashi, Yoko Taruno, Takahiro Kanai, Junji Fukuda
Self-assembled monolayers (SAMs) have been used to elucidate interactions between cells and material surface chemistry. Gold surfaces modified with oligopeptide SAMs exhibit several unique characteristics, such as cell-repulsive surfaces, micropatterns of cell adhesion and non-adhesion regions for control over cell microenvironments, and dynamic release of cells upon external stimuli under culture conditions. However, basic procedures for the preparation of oligopeptide SAMs, including appropriate cleaning methods of the gold surface before modification, have not been fully established...
January 15, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29352273/binder-free-sheet-type-all-solid-state-batteries-with-enhanced-rate-capabilities-and-high-energy-densities
#2
Mari Yamamoto, Yoshihiro Terauchi, Atsushi Sakuda, Masanari Takahashi
All-solid-state batteries using inorganic solid electrolytes are considered promising energy storage systems because of their safety and long life. Stackable and compact sheet-type all-solid-state batteries are urgently needed for industrial applications such as smart grids and electric vehicles. A binder is usually indispensable to the construction of sheet-type batteries; however, it can decrease the power and cycle performance of the battery. Here we report the first fabrication of a binder-free sheet-type battery...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29352135/blocking-zika-virus-vertical-transmission
#3
Pinar Mesci, Angela Macia, Spencer M Moore, Sergey A Shiryaev, Antonella Pinto, Chun-Teng Huang, Leon Tejwani, Isabella R Fernandes, Nicole A Suarez, Matthew J Kolar, Sandro Montefusco, Scott C Rosenberg, Roberto H Herai, Fernanda R Cugola, Fabiele B Russo, Nicholas Sheets, Alan Saghatelian, Sujan Shresta, Jeremiah D Momper, Jair L Siqueira-Neto, Kevin D Corbett, Patricia C B Beltrão-Braga, Alexey V Terskikh, Alysson R Muotri
The outbreak of the Zika virus (ZIKV) has been associated with increased incidence of congenital malformations. Although recent efforts have focused on vaccine development, treatments for infected individuals are needed urgently. Sofosbuvir (SOF), an FDA-approved nucleotide analog inhibitor of the Hepatitis C (HCV) RNA-dependent RNA polymerase (RdRp) was recently shown to be protective against ZIKV both in vitro and in vivo. Here, we show that SOF protected human neural progenitor cells (NPC) and 3D neurospheres from ZIKV infection-mediated cell death and importantly restored the antiviral immune response in NPCs...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29349490/auto-aggregation-properties-of-a-novel-aerobic-denitrifier-enterobacter-sp-strain-fl
#4
Xia Wang, Qiang An, Bin Zhao, Jin Song Guo, Yuan Sheng Huang, Meng Tian
Enterobacter sp. strain FL was newly isolated from activated sludge and exhibited significant capability of auto-aggregation as well as aerobic denitrification. The removal efficiencies of NO3--N, total nitrogen (TN), and TOC by strain FL in batch culture reached 94.6, 63.9, and 72.5% in 24 h, respectively. The production of N2O and N2 in the presence of oxygen demonstrated the occurrence of aerobic denitrification. The auto-aggregation index of strain FL reached 54.3%, suggesting a high tendency that the cells would agglomerate into aggregates...
January 18, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29346724/structural-and-biophysical-characterization-of-human-extl3-domain-organisation-glycosylation-and-solution-structure
#5
Wael Awad, Sven Kjellstrom, Gabriel Svensson Birkedal, Katrin Mani, Derek Thomas Logan
Heparan sulfate proteoglycans are proteins substituted with one or more heparan sulfate (HS) polysaccharides, found in abundance at cell surfaces. HS chains influence the activity of many biologically important molecules involved in cellular communication and signaling. The exostosin (EXT) proteins are glycosyltransferases in the Golgi apparatus that assemble HS chains on HSPGs. The EXTL3 enzyme mainly works as an initiator in HS biosynthesis. In this work, human lumenal N-glycosylated EXTL3 (EXTL3ΔN) was cloned, expressed in human embryonic kidney cells and purified...
January 18, 2018: Biochemistry
https://www.readbyqxmd.com/read/29345306/hypoalbuminaemia-segregates-different-prognostic-subgroups-within-the-refined-standard-risk-acute-graft-versus-host-disease-score
#6
Mohamed A Kharfan-Dabaja, Kyle Sheets, Ambuj Kumar, Hemant S Murthy, Taiga Nishihori, Athanasios Tsalatsanis, Alain Mina, John Mathews, Ernesto Ayala, Julio Chavez, Lia E Perez, Brian C Betts, Claudio Anasetti, Joseph Pidala
Hypoalbuminaemia has been previously described to predict worse non-relapse mortality (NRM) and inferior overall survival (OS) in allogeneic haematopoietic cell transplant (allo-HCT) recipients. Here, we evaluate the role of hypoalbuminaemia (<35 g/l) at time of onset of acute graft-versus-host disease (aGVHD) when incorporated into the refined aGVHD score. The study population consisted of 522 patients, median age 53 (18-75) years, who underwent an allo-HCT mostly for haematological malignancies. Standard risk (SR) aGVHD comprised 467 patients (89%) and the number of high risk (HR) cases was 55 (11%)...
January 18, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/29345093/rapid-fabrication-of-detachable-three-dimensional-tissues-by-layering-of-cell-sheets-with-heating-centrifuge
#7
Yuji Haraguchi, Yuki Kagawa, Akiyuki Hasegawa, Hirotsugu Kubo, Tatsuya Shimizu
Confluent cultured cells on a temperature-responsive culture dish can be harvested as an intact cell sheet by decreasing temperature below 32°C. A three-dimensional (3-D) tissue can be fabricated by the layering of cell sheets. A resulting 3-D multi-layered cell sheet-tissue on a temperature-responsive culture dish can be also harvested without any damage by only temperature decreasing. For shortening the fabrication time of the 3-D multi-layered constructs, we attempted to layer cell sheets on a temperature-responsive culture dish with centrifugation...
January 18, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29345038/electrocatalytic-activity-of-2d-phosphorene-based-heteroelectrocatalyst-for-photoelectrochemical-cells
#8
Munkhbayar Batmunkh, Aabhash Shrestha, Munkhjargal Bat-Erdene, Md Julker Nine, Cameron James Shearer, Christopher Gibson, Ashley Slattery, Sherif Tawfik, Michael Ford, Sheng Dai, Shizhang Qiao, Joseph George Shapter
Research into efficient synthesis, fundamental properties and potential applications of phosphorene is currently the subject of intense investigation. Herein, solution processed phosphorene or few-layer black phosphorus (FL-BP) sheets are prepared using microwave-exfoliation method and used in photoelectrochemical cells. Based on our experimental and theoretical (DFT) studies, we found that the FL-BP sheets can act as catalytically active sites and show excellent electrocatalytic activity for triiodide reduction in solar cells...
January 17, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29344766/patient-reported-symptoms-before-palliative-radiotherapy-predict-survival-differences
#9
Carsten Nieder, Thomas A Kämpe, Adam Pawinski, Astrid Dalhaug
BACKGROUND: Widely used prognostic scores, e. g., for brain or bone metastases, are based on disease- and patient-related factors such as extent of metastases, age and performance status, which were available in the databases used to develop the scores. Few groups were able to include patient-reported symptoms. In our department, all patients were assessed with the Edmonton Symptom Assessment System (ESAS, a one-sheet questionnaire addressing 11 major symptoms and wellbeing on a numeric scale of 0-10) at the time of treatment planning since 2012...
January 17, 2018: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
https://www.readbyqxmd.com/read/29344160/the-role-of-computed-tomography-and-magnetic-resonance-imaging-in-diagnosing-clear-cell-ameloblastoma-a-case-report
#10
Shoko Gamoh, Masahiro Wato, Hironori Akiyama, Kaname Tsuji, Hiroki Ishikawa, Kaori Naruse, Hiroaki Yoshida, Shosuke Morita, Akio Tanaka, Kimishige Shimizutani
Ameloblastoma is the most common and clinically relevant type of odontogenic tumor. Clear cell odontogenic carcinoma is histologically characterized by solid sheets and nests of clear cells, whereas clear cell ameloblastoma (CCAM) is histologically characterized by an ameloblastomatous component intermixed with an extensive clear cell component. A total of 12 reports have been published on the histological etiology for CCAM; however, no reports have made regarding the detailed computed tomography and/or magnetic resonance imaging features of tumors of this type...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29341617/enhancing-docetaxel-delivery-to-multidrug-resistant-cancer-cells-with-albumin-coated-nanocrystals
#11
Sheryhan F Gad, Joonyoung Park, Ji Eun Park, Gihan N Fetih, Sozan S Tous, Wooin Lee, Yoon Yeo
Intravenous delivery of poorly water-soluble anticancer drugs such as docetaxel (DTX) is challenging due to the low bioavailability and the toxicity related to solubilizing excipients. Colloidal nanoparticles are used as alternative carriers, but low drug loading capacity and circulation instability limit their clinical translation. To address these challenges, DTX nanocrystals (NCs) were prepared using Pluronic F127 as an intermediate stabilizer and albumin as a functional surface modifier, which were previously found to be effective in producing small and stable NCs...
January 17, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29340391/graphene-on-116-faceted-monocrystalline-anatase-nanosheet-array-for-ultraviolet-detection
#12
Yu Liu, Honglei Cai, Fangfang Wang, Jianlin Wang, Qiuping Huang, Zhengping Fu, Yalin Lu
A structure composed of a nanosheet array may trap photons, which could be used to enhance the optical response via resonances; this may be highly useful in ultraviolet (UV) detection, photocatalysis, solar cells, etc. Moreover, anatase nanosheets exposed with the active {001}, {111}, and {116} facets are promising in applications such as Li-ion battery, photocatalysis, and electrochemistry. Therefore, in this study, the {116} faceted single-crystalline anatase nanosheet array with the sheet spacing in the range of several hundred nanometers was directly grown on the transparent conductive substrates...
January 17, 2018: Nanoscale
https://www.readbyqxmd.com/read/29339149/use-of-skinethic%C3%A2-rhe-for-in-vitro-evaluation-of-skin-irritation-of-medical-device-extracts
#13
Christian Pellevoisin, Christelle Videau, Damien Briotet, Corinne Grégoire, Carine Tornier, Alain Alonso, Anne Sophie Rigaudeau, Charbel Bouez, Nathalie Seyler
According to ISO 10993 standards for biocompatibility of medical devices, skin irritation is one of the three toxicological endpoints to be always addressed in a biological risk assessment. This work presents a new protocol to assess this endpoint in vitro rather than in vivo. The protocol was adapted to medical devices extracts from the OECD TG 439 with the SkinEthic™ RHE model as test system. It was challenged with irritant chemicals, Sodium Dodecyl Sulfate, Lactic Acid and Heptanoic Acid spiked in polar solvents, sodium chloride solution or phosphate buffer saline and non-polar solvent, Sesame Oil...
January 12, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29337067/the-impact-of-growth-factors-on-human-induced-pluripotent-stem-cells-differentiation-into-cardiomyocytes
#14
Marianne E Yassa, Iman A Mansour, Nadia I Sewelam, Hala Hamza, Taghrid Gaafar
Human induced pluripotent stem cells (hiPSCs) act as a promising therapeutic alternative for cardiovascular diseases. They yield a large number of functional cardiomyocytes (CMs) from autologous cell sources without ethical or immunological problems. However, significant limitations still remain in terms of line-to-line variability in CM yield and reproducibility. AIM: To efficiently enhance NP0040 hiPSCs differentiation into CMs. MAIN METHODS: Following a standard cardiac differentiation protocol using small molecules targeting the canonical Wnt signaling, growth factors (BMP4 and FGF2) and ascorbic acid were added further in order to increase the cardiac differentiation efficiency...
January 11, 2018: Life Sciences
https://www.readbyqxmd.com/read/29337044/lysophosphatidic-acid-regulates-the-motility-of-mcf10ca1a-breast-cancer-cell-sheets-via-two-opposing-signaling-pathways
#15
Christina H Stuelten, Rachel M Lee, Wolfgang Losert, Carole A Parent
Aberrant cell migration leads to the dispersal of malignant cells. The ubiquitous lipid mediator lysophosphatidic acid (LPA) modulates cell migration and is implicated in tumor progression. Yet, the signaling cascades that regulate LPA's effect on cell motility remain unclear. Using time-lapse imaging and quantitative analyses, we studied the role of signaling cascades that act downstream of LPA on the motility of MCF10CA1a breast cancer cells. We found that LPA alters cell motility via two major signaling pathways...
January 11, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29333326/evaluation-of-the-periodontal-regenerative-properties-of-patterned-human-periodontal-ligament-stem-cell-sheets
#16
Joong-Hyun Kim, Seok-Yeong Ko, Justin Ho Lee, Deok-Ho Kim, Jeong-Ho Yun
Purpose: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum. Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet. Results: The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum...
December 2017: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/29331337/elastin-in-lung-development-and-disease-pathogenesis
#17
REVIEW
Robert P Mecham
Elastin is expressed in most tissues that require elastic recoil. The protein first appeared coincident with the closed circulatory system, and was critical for the evolutionary success of the vertebrate lineage. Elastin is expressed by multiple cell types in the lung, including mesothelial cells in the pleura, smooth muscle cells in airways and blood vessels, endothelial cells, and interstitial fibroblasts. This highly crosslinked protein associates with fibrillin-containing microfibrils to form the elastic fiber, which is the physiological structure that functions in the extracellular matrix...
January 10, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29330844/bradykinin-mediates-the-association-of-collecting-duct-cells-to-form-migratory-colonies-through-b2-receptor-activation
#18
Edith Del Valle Guaytima, Yamila Romina Brandán, Nicolás Octavio Favale, Bruno Jaime Santacreu, Norma B Sterin-Speziale, María Gabriela Márquez
It is known that bradykinin (BK) B2 receptor (B2R) is expressed in the collecting duct (CD) cells of the newborn rat kidney, but little is known about its role during early postnatal life. Therefore, we hypothesize that BK could participate in the mechanisms that mediate CD formation during the postnatal renal development. Performing primary cultures, combined with biochemical, immunocytochemical, and time-lapse analysis, we studied the role of BK in CD cell behaviour isolated from renal papilla of neonatal rats...
January 13, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29330069/conformation-and-trimer-association-of-the-transmembrane-domain-of-the-parainfluenza-virus-fusion-protein-in-lipid-bilayers-from-solid-state-nmr-insights-into-the-sequence-determinants-of-trimer-structure-and-fusion-activity
#19
Myungwoon Lee, Hongwei Yao, Byungsu Kwon, Alan J Waring, Peter Ruchala, Chandan Singh, Mei Hong
Enveloped viruses enter cells by using their fusion proteins to merge the virus lipid envelope and the cell membrane. While crystal structures of the water-soluble ectodomains of many viral fusion proteins have been determined, the structure and assembly of the C-terminal transmembrane domain (TMD) remains poorly understood. Here we use solid-state NMR to determine the backbone conformation and oligomeric structure of the TMD of the parainfluenza virus 5 (PIV5) fusion protein. 13C chemical shifts indicate that the central leucine-rich segment of the TMD is α-helical in POPC/cholesterol membranes and POPE membranes, while the Ile- and Val-rich termini shift to the β-strand conformation in the POPE membrane...
January 9, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29329001/improved-antibacterial-activity-of-a-marine-peptide-n2-against-intracellular-salmonella-typhimurium-by-conjugating-with-cell-penetrating-peptides-blfcin6-tat11
#20
Zhanzhan Li, Xiao Wang, Da Teng, Ruoyu Mao, Ya Hao, Na Yang, Huixian Chen, Xiumin Wang, Jianhua Wang
Salmonellae, gram-negative bacteria, are facultative intracellular pathogens that cause a number of diseases in animals and humans. The poor penetration ability of antimicrobial agents limits their use in the treatment of intracellular bacterial infections. In this study, the cell-penetrating peptides (CPPs) bLFcin6 and Tat11 were separately conjugated to the antimicrobial peptide N2, and the antibacterial activity and pharmacodynamics of the CPPs-N2 conjugates were first evaluated against Salmonellae typhimurium in vitro and in macrophage cells...
December 29, 2017: European Journal of Medicinal Chemistry
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