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https://www.readbyqxmd.com/read/28040413/in-search-of-an-improved-injection-technique-for-the-clinical-application-of-spermatogonial-stem-cell-transplantation
#1
Katrien Faes, Tony Lahoutte, Anne Hoorens, Herman Tournaye, Ellen Goossens
When fertility is impaired by anticancer treatment, spermatogonial stem cell transplantation (SSCT) could be used as a fertility restoration technique later on in life. Previously, we have demonstrated that a testicular cell suspension could be injected into a human cadaver testis, however, leakage to the interstitium was observed. In this study, injection of mouse testicular cells at an injection height of 50 cm (hydrostatic pressure) or via an automated injection pump (1400 µl, 2600 µl and 3000 µl) was evaluated...
December 15, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28024304/nasal-unit-transplantation-a-cadaveric-anatomical-feasibility-study
#2
Amir H Dorafshar, Gerhard S Mundinger, Brent Robinson, Sami Tuffaha, Gerald Brandacher, Patrick Byrne, Robert L Walton
Background The science and technical acumen in the field of vascularized composite allotransplantation has progressed rapidly over the past 15 years, and transplantation of specialized units of the face, such as the nose, appears possible. No study to date has evaluated the technical feasibility of isolated nasal unit transplantation (NUT). In this study, we explore the anatomy and technical specifics of NUT. Methods In this study, four fresh cadaver heads were studied. Bilateral vascular pedicle dissections were performed in each cadaver...
December 26, 2016: Journal of Reconstructive Microsurgery
https://www.readbyqxmd.com/read/27997448/the-retroauricular-approach-to-the-facial-nerve-trunk
#3
Jordan W Swanson, Jason W Yu, Jesse A Taylor, Stephen Kovach, Suhail Kanchwala, Laurent Lantieri
BACKGROUND: Exposure of the common trunk of the facial nerve has traditionally been approached based on principles of parotidectomy, which is associated with high rates of facial nerve palsy and landmarks that may be unreliable. On the basis of experience gained with vascularized composite allotransplantation of the face, the authors propose a retroauricular approach that may be more time-effective and safe. METHODS: In the proposed retroauricular facial nerve approach, an incision is made posterior to the ear in the retroauricular sulcus, and dissection proceeds anteriorly to the mastoid fascia to the base of the conchal bowl...
December 16, 2016: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/27893628/watershed-areas-in-face-transplantation
#4
Bahar Bassiri Gharb, Russell S Frautschi, Brianna C Halasa, Gaby D Doumit, Risal S Djohan, Steven L Bernard, Brian R Gastman, Maria Z Siemionow, Francis A Papay, Antonio Rampazzo
BACKGROUND: Maxillary artery has traditionally been considered the main blood supply of the facial skeleton. However, the deep and concealed location makes the harvest of facial allografts based on this artery challenging, giving preference to the facial artery. There is growing evidence that the junction between the hard and soft palate may represent a watershed area in facial artery based allografts. The aim of this study was to review the occurrence of partial allograft necrosis and modify the available craniofacial techniques allowing for a reliable harvest of maxillary artery based facial allografts...
November 21, 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27805444/feasibility-repeatability-and-safety-of-ultrasound-guided-stimulation-of-the-first-cervical-nerve-at-the-alar-foramen-in-horses
#5
Céline Mespoulhès-Rivière, Olivier Brandenberger, Fabrice Rossignol, Céline Robert, Justin D Perkins, Jean-Paul Marie, Norm Ducharme
OBJECTIVE To develop and assess the feasibility, repeatability, and safety of an ultrasound-guided technique to stimulate the first cervical nerve (FCN) at the level of the alar foramen of the atlas of horses. ANIMALS 4 equine cadavers and 6 clinically normal Standardbreds. PROCEDURES In each cadaver, the FCN pathway was determined by dissection, and any anastomosis between the first and second cervical nerves was identified. Subsequently, each of 6 live horses underwent a bilateral ultrasound-guided stimulation of the FCN at the alar foramen 3 times at 3-week intervals...
November 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27789209/three-dimensional-printing-for-restoration-of-the-donor-face-a-new-digital-technique-tested-and-used-in-the-first-facial-allotransplantation-patient-in-finland
#6
A A Mäkitie, M Salmi, A Lindford, J Tuomi, P Lassus
BACKGROUND AND AIMS: Prosthetic mask restoration of the donor face is essential in current facial transplant protocols. The aim was to develop a new three-dimensional (3D) printing (additive manufacturing; AM) process for the production of a donor face mask that fulfilled the requirements for facial restoration after facial harvest. MATERIALS AND METHODS: A digital image of a single test person's face was obtained in a standardized setting and subjected to three different image processing techniques...
October 5, 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/27747235/effect-of-prophylactic-antifungal-protocols-on-the-prognosis-of-liver-transplantation-a-propensity-score-matching-and-multistate-model-approach
#7
Yi-Chan Chen, Ting-Shuo Huang, Yu-Chao Wang, Chih-Hsien Cheng, Chen-Fang Lee, Ting-Jun Wu, Hong-Shiue Chou, Kun-Ming Chan, Wei-Chen Lee, Ruey-Shyang Soong
Background. Whether routine antifungal prophylaxis decreases posttransplantation fungal infections in patients receiving orthotopic liver transplantation (OLT) remains unclear. This study aimed to determine the effectiveness of antifungal prophylaxis for patients receiving OLT. Patients and Methods. This is a retrospective analysis of a database at Chang Gung Memorial Hospital. We have been administering routine antibiotic and prophylactic antifungal regimens to recipients with high model for end-stage liver disease scores (>20) since 2009...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27742327/looking-for-new-alternatives-what-nursing-students-of-lodz-s-medical-university-in-poland-think-about-the-use-of-organs-coming-from-animals
#8
M Mikla, A Rios, A Lopez-Navas, D Kilanska, B Gorzkowicz, L Martinez-Alarcón, G Ramis, P Ramirez, M J Lopez Montesinos
BACKGROUND: The shortage of organs has made it necessary to look for new alternatives as xenotransplantation. The objective of this study was to analyze the attitudes of Polish nursing students toward organ donation of nonhuman origin, and to determine the factors that affect them. METHODS: Year 2011-2012. Nursing students of the University of Poland. Sampling points in 5 compulsory-attendance nursing courses with a completion rate of fulfillment of 80%. Validated questionnaire (PCID-XenoTx Rios) filled out anonymously and self-administered...
September 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27740958/the-spinal-accessory-nerve-for-functional-muscle-innervation-in-facial-reanimation-surgery-an-anatomical-and-histomorphometric-study
#9
Eva Placheta, Ines Tinhofer, Melanie Schmid, Lukas F Reissig, Igor Pona, Wolfgang Weninger, Thomas Rath, David Chwei-Chin Chuang, Chieh-Han John Tzou
INTRODUCTION: Facial reanimation surgery is performed in severe cases of facial palsy to restore facial function. In a 1-stage procedure, the spinal accessory nerve can be used as a donor nerve to power a free gracilis muscle transplant for the reanimation of the mouth. The aim of this study was to describe the surgical anatomy of the spinal accessory nerve, provide a guide for reliable donor nerve dissection, and analyze the available donor axon counts. METHODS: Dissections were performed on 10 nonembalmed cadavers (measurements of 20 nerves)...
October 6, 2016: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/27668589/generation-of-pure-cultures-of-autologous-schwann-cells-by-use-of-biopsy-specimens-of-the-dorsal-cutaneous-branches-of-the-cervical-nerves-of-young-adult-dogs
#10
Ji-Hey Lim, Natasha J Olby
OBJECTIVE To identify an optimal technique for isolation, purification, and amplification of Schwann cells (SCs) from biopsy specimens of the dorsal cutaneous branches of the cervical nerves of dogs. SAMPLE Biopsy specimens of dorsal cervical cutaneous nerves from the cadavers of three 1- to 2-year-old dogs. PROCEDURES Nerve specimens were dissected, predegenerated, and dissociated to isolate single cells. After culture to enhance SC growth, cells were immunopurified by use of magnetic beads. Cell purity was evaluated by assessing expression of cell surface antigens p75 (to detect SCs) and CD90 (to detect fibroblasts)...
October 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27559289/quantitative-proteomics-analysis-with-itraq-in-human-lenses-with-nuclear-cataracts-of-different-axial-lengths
#11
Haiyan Zhou, Hong Yan, Weijia Yan, Xinchuan Wang, Yong Ma, Jianping Wang
PURPOSE: The goal of this study was to identify and quantify the differentially expressed proteins in human nuclear cataract with different axial lengths. METHODS: Thirty-six samples of human lens nuclei with hardness grade III or IV were obtained during cataract surgery with extracapsular cataract extraction (ECCE). Six healthy transparent human lens nuclei were obtained from fresh healthy cadaver eyes during corneal transplantation surgery. The lens nuclei were divided into seven groups (six lenses in each group) according to the optic axis: Group A (mean axial length 28...
2016: Molecular Vision
https://www.readbyqxmd.com/read/27517508/development-and-refinement-of-computer-assisted-planning-and-execution-system-for-use-in-face-jaw-teeth-transplantation-to-improve-skeletal-and-dento-occlusal-outcomes
#12
Sepehr Hashemi, Mehran Armand, Chad R Gordon
PURPOSE OF REVIEW: To describe the development and refinement of the computer-assisted planning and execution (CAPE) system for use in face-jaw-teeth transplants (FJTTs). RECENT FINDINGS: Although successful, some maxillofacial transplants result in suboptimal hybrid occlusion and may require subsequent surgical orthognathic revisions. Unfortunately, the use of traditional dental casts and splints pose several compromising shortcomings in the context of FJTT and hybrid occlusion...
October 2016: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/27504274/unusual-variation-in-the-branching-pattern-of-the-celiac-trunk-and-its-embryological-and-clinical-perspective
#13
Shilpi Agarwal, Babita Pangtey, Neelam Vasudeva
Knowledge of variations in the branching pattern of Celiac Trunk (CT) is important for surgeries of stomach, duodenum, pancreas and hepatobiliary region, for abdominal aortic aneurysm, for liver transplant and chaemoembolization of hepatic tumours. An unusual variation in the branching pattern of CT was observed in 50-year-old male cadaver of Indian origin. CT divided into six branches out of which right & left inferior phrenic arteries originated as first two branches and left gastric artery as the third branch...
June 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27493934/cost-effectiveness-analysis-of-dialysis-and-kidney-transplant-in-patients-with-renal-impairment-using-disability-adjusted-life-years-in-iran
#14
Safiye YaghoubiFard, Reza Goudarzi, Abbas Etminan, MohammadReza Baneshi, Mohsen Barouni, Mohammad Jafari Sirizi
BACKGROUND: This cross-sectional study was conducted to compare the cost-effectiveness of three therapeutic methods of long-term hemodialysis, kidney transplant from a living person and kidney transplant from a cadaver utilizing Disability Adjusted Life Years (DALY) using data from the records of patients referred to Afzalipour Hospital of Kerman in 2012. METHODS: This cross-sectional study utilizing Disability Adjusted Life Years (DALY) as outcome measure, used data from the records of patients referred to Afzalipour Hospital of Kerman in 2012...
2016: Medical Journal of the Islamic Republic of Iran
https://www.readbyqxmd.com/read/27468207/doppler-ultrasonography-in-living-donor-liver-transplantation-recipients-intra-and-post-operative-vascular-complications
#15
REVIEW
Omar Abdelaziz, Hussein Attia
Living-donor liver transplantation has provided a solution to the severe lack of cadaver grafts for the replacement of liver afflicted with end-stage cirrhosis, fulminant disease, or inborn errors of metabolism. Vascular complications remain the most serious complications and a common cause for graft failure after hepatic transplantation. Doppler ultrasound remains the primary radiological imaging modality for the diagnosis of such complications. This article presents a brief review of intra- and post-operative living donor liver transplantation anatomy and a synopsis of the role of ultrasonography and color Doppler in evaluating the graft vascular haemodynamics both during surgery and post-operatively in accurately defining the early vascular complications...
July 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27383457/cost-comparison-of-kidney-transplant-versus-dialysis-in-brazil
#16
Silvia Brand Silva, Heitor Mansur Caulliraux, Claudia Affonso Silva Araújo, Eduardo Rocha
This study aimed to compare the direct medical costs of renal transplantation and renal replacement therapies, specifically hemodialysis and peritoneal dialysis, from the perspective of the Brazilian Unified National Health System (SUS). Renal replacement therapies costs were based on data published in the literature. Cost items for kidney transplant were identified in a private hospital based on procedure codes used for charging the SUS, and other items were taken from the literature. In the four years covered by the study, cadaver kidney transplant generated per-patient savings of BRL 37,000 and BRL 74,000 compared to hemodialysis and peritoneal dialysis, respectively...
June 1, 2016: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/27363540/technical-skills-required-in-split-liver-transplantation
#17
Huanqiu Liu, Ruijun Li, Jinling Fu, Qianyan He, Ji Li
The number of liver grafts obtained from a cadaver can be greatly increased with the application of split liver transplantation. In the last 10 years, pediatric waiting list mortality has been reduced significantly with the use of this form of liver transplantation, which has 2 major forms. In its most commonly used form, the liver can be transplanted into 1 adult and 1 child by splitting it into a right extended and a left lateral graft. For adult and pediatric recipients, the results of this procedure are comparable to those of whole-organ techniques...
July 1, 2016: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/27329292/deceased-tissue-donor-serology-and-molecular-testing-for-hiv-hepatitis-b-and-hepatitis-c-viruses-a-lack-of-cadaveric-validated-tests
#18
REVIEW
Thayssa Neiva da Fonseca Victer, Cris Stéphany Rodrigues Dos Santos, Sônia Nair Báo, Thatiane Lima Sampaio
Vital to patient safety is the accurate assessment and minimization of risk for human immunodeficiency virus (HIV), Hepatitis C (HCV), and Hepatitis B (HBV) virus transmission by deceased donor organ and tissue transplantation. The pathogens are tested by serological kits based on enzyme-linked immunosorbent assay (ELISA), chemiluminescence (CLIA) and eletrochemiluminescence (ECLIA) immunoassays. Organ transplantation is a highly successful life-saving treatment in Brazil, but the Brazilian Health Surveillance Agency currently mandates that all deceased organ donors are screened for HIV, HCV and HBV following living donor policies...
December 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27238991/omental-flow-through-flap-experimental-hemodynamic-study
#19
Nicla Settembre, Sergueï Malikov
BACKGROUND: The omental flow-through flap (OFTF) is based on the use of an anatomic unit composed of the right gastro-omental artery (bypass) with its omental branch or branches supplying the greater omentum (flap). The greater omentum flap is known for its capacity of resistance to infection, for its use in the treatment of ischemic lesions and as a high-flow tissue. Several hypotheses regarding the hemodynamic behavior of a distal bypass with a flap were discussed in the literature...
October 2016: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/27178491/unrelated-renal-transplantation-an-ethical-enigma
#20
Gaurav Aggarwal, Samiran Adhikary
End-stage renal disease (ESRD) is a condition better discussed than suffered. People suffering from ESRD are at a disadvantage not only financially, but also emotionally and in terms of the quality of their lives. The majority of their productive time is spent in hospital, on dialysis machines, or in the search for a suitable kidney donor, so that they may be able to improve upon the quality of their remaining lifespan. Only a "lucky few" are able to find a suitable matching donor, be it living (related) or a cadaver, whilst the others are left to fend for themselves...
July 2016: Indian Journal of Medical Ethics
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