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https://www.readbyqxmd.com/read/29232144/interactions-between-surface-immobilized-antimicrobial-peptides-and-model-bacterial-cell-membranes
#1
Xiaofeng Han, Jingguo Zheng, Fengming Lin, Kenichi Kuroda, Zhan Chen
Sum frequency generation vibrational spectroscopy (SFG) was used to study surface immobilization effects on the interactions between antimicrobial peptide (AMP) cecropin P1 (CP1) and model cell membranes. While free CP1 in solution interacted with a model cell membrane composed of a phosphatidylglycerole (PG) bilayer, electrostatic interaction led to the attachment of the CP1 molecules onto the PG surface, and the hydrophobic domain in the lipid bilayer enabled the peptides to insert into the bilayer and form α-helices from random coil structures...
December 12, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29231793/acute-rerupture-after-coil-embolization-of-ruptured-intracranial-saccular-aneurysms-a-literature-review
#2
Kailing Li, Yunbao Guo, Ying Zhao, Baofeng Xu, Kan Xu, Jinlu Yu
Acute rerupture after coil embolization is defined as rerupture within three days after treatment; its prognosis is worse than that of rebleeding at other time periods. However, to date, little is known about complications during the acute phase. Therefore, we used the PubMed database to perform a review of acute rerupture after coil embolization of ruptured intracranial saccular aneurysms and increase our understanding. After reviewing the complications, we found that the cause of acute rerupture is unclear, but the following risk factors are involved: incomplete occlusion of the initial aneurysm, the presence of a hematoma adjacent to a ruptured aneurysm, an aneurysmal outpouching, poor Hunt-Hess grade at the time of treatment, and the location of the aneurysm in an anterior communicating artery...
January 1, 2017: Interventional Neuroradiology
https://www.readbyqxmd.com/read/29230860/cardiac-gating-using-scattering-of-an-8-channel-parallel-transmit-coil-at-7t
#3
Sven H F Jaeschke, Matthew D Robson, Aaron T Hess
PURPOSE: To establish a cardiac signal from scattering matrix or scattering coefficient measurements made on a 7T 8-channel parallel transmit (pTx) system, and to evaluate its use for cardiac gating. METHODS: Measurements of the scattering matrix and scattering coefficients were acquired using a monitoring pulse sequence and during a standard cine acquisition, respectively. Postprocessing used an independent component analysis and gating feature identification. The effect of the phase of the excitation radiofrequency (RF) field ( B1+ shim) on the cardiac signal was simulated for multiple B1+ shim configurations, and cine images were reconstructed from both the scattering coefficients and electrocardiogram (ECG)...
December 11, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29230450/charge-evolution-during-the-unfolding-of-a-single-dna-i-motif
#4
Weibin Ren, Kaikai Zheng, Charan Liao, Jingfa Yang, Jiang Zhao
The effective charge and evolution of single chains of a DNA i-motif during its unfolding process are investigated at the single molecule level. Using fluorescence correlation spectroscopy and photon counting histograms, the single chain dimensions and electrical potential of cytosine-rich human telomeric oligonucleotides are monitored, during their unfolding from the i-motif to the random coil state. It is discovered that the effective charge density of the DNA chain is very sensitive to conformation changes and the results remarkably expose the existence of an intermediate state of the unfolding process...
December 12, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29230422/novel-semiquantitative-bone-marrow-oedema-score-and-fracture-score-for-the-magnetic-resonance-imaging-assessment-of-the-active-charcot-foot-in-diabetes
#5
L Meacock, N L Petrova, Ana Donaldson, A Isaac, A Briody, R Ramnarine, M E Edmonds, D A Elias
There are no accepted methods to grade bone marrow oedema (BMO) and fracture on magnetic resonance imaging (MRI) scans in Charcot osteoarthropathy. The aim was to devise semiquantitative BMO and fracture scores on foot and ankle MRI scans in diabetic patients with active osteoarthropathy and to assess the agreement in using these scores. Three radiologists assessed 45 scans (Siemens Avanto 1.5T, dedicated foot and ankle coil) and scored independently twenty-two bones (proximal phalanges, medial and lateral sesamoids, metatarsals, tarsals, distal tibial plafond, and medial and lateral malleoli) for BMO (0-no oedema, 1-oedema < 50% of bone volume, and 2-oedema > 50% of bone volume) and fracture (0-no fracture, 1-fracture, and 2-collapse/fragmentation)...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/29230304/coil-embolization-for-intrahepatic-haemorrhage-following-liver-biopsy-in-a-patient-with-hepatitis-c-virus-infection-and-hepatic-microaneurysms
#6
Apostolos Koffas, John Karani, Roger Williams
Intrahepatic bleeding secondary to rupture of hepatic microaneurysms is an uncommon clinical entity more frequently associated with polyarteritis nodosa (PAN) or rarely with other vasculitis or autoimmune disease. Hepatic vasculitis is reported in chronic hepatitis C virus (HCV) infection and an association between hepatitis C and PAN is described. The current report presents the case of a middle-aged female patient with a medical history remarkable for HCV infection who underwent a percutaneous liver biopsy, which was complicated by severe intrahepatic and perihepatic haemorrhage...
December 2017: Oxford Medical Case Reports
https://www.readbyqxmd.com/read/29230178/human-cardiac-31p-mr-spectroscopy-at-3-tesla-cannot-detect-failing-myocardial-energy-homeostasis-during-exercise
#7
Adrianus J Bakermans, Jason N Bazil, Aart J Nederveen, Gustav J Strijkers, S Matthijs Boekholdt, Daniel A Beard, Jeroen A L Jeneson
Phosphorus-31 magnetic resonance spectroscopy (31P-MRS) is a unique non-invasive imaging modality for probing in vivo high-energy phosphate metabolism in the human heart. We investigated whether current 31P-MRS methodology would allow for clinical applications to detect exercise-induced changes in (patho-)physiological myocardial energy metabolism. Hereto, measurement variability and repeatability of three commonly used localized 31P-MRS methods [3D image-selected in vivo spectroscopy (ISIS) and 1D ISIS with 1D chemical shift imaging (CSI) oriented either perpendicular or parallel to the surface coil] to quantify the myocardial phosphocreatine (PCr) to adenosine triphosphate (ATP) ratio in healthy humans (n = 8) at rest were determined on a clinical 3 Tesla MR system...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29230164/a-sub-millimeter-inductively-powered-neural-stimulator
#8
Daniel K Freeman, Jonathan M O'Brien, Parshant Kumar, Brian Daniels, Reed A Irion, Louis Shraytah, Brett K Ingersoll, Andrew P Magyar, Andrew Czarnecki, Jesse Wheeler, Jonathan R Coppeta, Michael P Abban, Ronald Gatzke, Shelley I Fried, Seung Woo Lee, Amy E Duwel, Jonathan J Bernstein, Alik S Widge, Ana Hernandez-Reynoso, Aswini Kanneganti, Mario I Romero-Ortega, Stuart F Cogan
Wireless neural stimulators are being developed to address problems associated with traditional lead-based implants. However, designing wireless stimulators on the sub-millimeter scale (<1 mm3) is challenging. As device size shrinks, it becomes difficult to deliver sufficient wireless power to operate the device. Here, we present a sub-millimeter, inductively powered neural stimulator consisting only of a coil to receive power, a capacitor to tune the resonant frequency of the receiver, and a diode to rectify the radio-frequency signal to produce neural excitation...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29229700/flexible-hinges-in-bacterial-chemoreceptors
#9
Narahari Akkaladevi, Filiz Bunyak, David Stalla, Tommi A White, Gerald L Hazelbauer
Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for formation of signaling complexes and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted receptors. Electron tomography of in vivo signaling complex arrays lack distinct densities for chemoreceptor rods away from the well-ordered base plate region, implying structural heterogeneity. We investigated using negative staining, transmission electron microscopy and image analysis to characterize molecular shapes of intact homodimers of Escherichia coli aspartate receptor Tar rendered functional by insertion into Nanodisc-provided E...
December 11, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29229286/whole-length-myosin-binding-protein-c-stabilizes-myosin-s2-as-measured-by-gravitational-force-spectroscopy
#10
Rohit R Singh, James W Dunn, Motamed M Qadan, Nakiuda Hall, Kathy K Wang, Douglas D Root
The mechanical stability of the myosin subfragment-2 (S2) was tested with simulated force spectroscopy (SFS) and gravitational force spectroscopy (GFS). Experiments examined unzipping S2, since it required less force than stretching parallel to the coiled coil. Both GFS and SFS demonstrated that the force required to destabilize the light meromyosin (LMM) was greater than the force required to destabilize the coiled coil at each of three different locations along S2. GFS data also conveyed that the mechanical stability of the S2 region is independent from its association with the myosin thick filament using cofilaments of myosin tail and a single intact myosin...
December 8, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29229244/the-functional-properties-and-structural-characteristics-of-deamidated-and-succinylated-wheat-gluten
#11
Yanhong Liu, Lingling Zhang, Yongxiang Li, Yang Yang, Fei Yang, Shuo Wang
Wheat gluten protein (WGP) was modified by deamidation and succinylation to improve its functional properties. The properties of deamidated and succinylated WGP were compared with those of unmodified WGP, deamidated WGP and succinylated WGP. Structural analysis was carried out with FTIR. Surface visualization was carried out with SEM and the thermal stability was determined by DSC. The results showed that an acylation level of 73.64% produced the optimal properties for modified WGP. After modification, its functional properties were significantly improved...
December 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29228292/recombination-directionality-factor-gp3-binds-%C3%AF-c31-integrase-via-the-zinc-domain-potentially-affecting-the-trajectory-of-the-coiled-coil-motif
#12
Paul C M Fogg, Ellen Younger, Booshini D Fernando, Thanafez Khaleel, W Marshall Stark, Margaret C M Smith
To establish a prophage state, the genomic DNA of temperate bacteriophages normally becomes integrated into the genome of their host bacterium by integrase-mediated, site-specific DNA recombination. Serine integrases catalyse a single crossover between an attachment site in the host (attB) and a phage attachment site (attP) on the circularized phage genome to generate the integrated prophage DNA flanked by recombinant attachment sites, attL and attR. Exiting the prophage state and entry into the lytic growth cycle requires an additional phage-encoded protein, the recombination directionality factor or RDF, to mediate recombination between attL and attR and excision of the phage genome...
December 8, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29227914/design-and-use-of-a-folded-four-ring-double-tuned-birdcage-coil-for-rat-brain-sodium-imaging-at-9-4%C3%A2-t
#13
YongHyun Ha, Chang-Hoon Choi, Wieland A Worthoff, Aliaksandra Shymanskaya, Michael Schöneck, Antje Willuweit, Jörg Felder, N Jon Shah
A folded four-ring quadrature birdcage coil was designed and constructed with a double-tune configuration of an outer high-pass coil for 1H (400 MHz) and inner low-pass coil for 23Na (105.72 MHz at 9.4 T). The coil was evaluated on the bench and in the scanner, comparing its performance with that of single-tuned coils and a large four-ring coil. All coils were tuned and matched and the isolation between two quadrature ports was found to be better than -13.7 dB for 1H and -27 dB for 23Na. Signal-to-noise ratios (SNRs) were calculated and 23Na flip angle maps were acquired...
December 6, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/29227890/a-novel-hydrophobically-modified-polyelectrolyte-for-enhanced-dewatering-of-clay-suspension
#14
Sarang P Gumfekar, João B P Soares
This work investigates the effect of multifunctional poly (N-isopropyl acrylamide/acrylic acid/N-tert-butylacrylamide) [p(NIPAM-AA-NTBA)] ternary polymer on the sedimentation of kaolin clay - a major fraction of oil sands tailings. A series of linear, uncross-linked p(NIPAM), p(NIPAM/AA), and p(NIPAM/AA/NTBM) were synthesized as random copolymers, where all monomer units were randomly arranged along the polymer backbone and connected by covalent bonds. The ternary copolymer, used as a flocculant, exhibited thermo-sensitivity, anionic nature, and hydrophobic association due to NIPAM, AA, and NTBM, respectively...
December 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/29227472/engineered-synthetic-scaffolds-for-organizing-proteins-within-the-bacterial-cytoplasm
#15
Matthew J Lee, Judith Mantell, Lorna Hodgson, Dominic Alibhai, Jordan M Fletcher, Ian R Brown, Stefanie Frank, Wei-Feng Xue, Paul Verkade, Derek N Woolfson, Martin J Warren
We have developed a system for producing a supramolecular scaffold that permeates the entire Escherichia coli cytoplasm. This cytoscaffold is constructed from a three-component system comprising a bacterial microcompartment shell protein and two complementary de novo coiled-coil peptides. We show that other proteins can be targeted to this intracellular filamentous arrangement. Specifically, the enzymes pyruvate decarboxylase and alcohol dehydrogenase have been directed to the filaments, leading to enhanced ethanol production in these engineered bacterial cells compared to those that do not produce the scaffold...
December 11, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/29227192/coiled-coil-motif-in-lbd16-and-lbd18-transcription-factors-are-critical-for-dimerization-and-biological-function-in-arabidopsis
#16
Shashank K Pandey, Jungmook Kim
The LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (LBD) gene family members encode a class of plant-specific transcription factors that play important roles in many different aspects of plant growth and development. The LBD proteins contain a conserved LOB domain harboring a Leu zipper-like coiled-coil motif, which has been predicted to mediate protein-protein interactions among the LBD family members. Dimerization of transcription factors is crucial for the modulation of their DNA-binding affinity, specificity, and diversity, contributing to the transcriptional regulation of distinct cellular and biological responses...
December 11, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/29227118/complex-suicide-involving-pyrethroid-ingestion-mosquito-coils-and-fatal-self-wounding-by-sharp-force
#17
Maria -Valeria Karakasi, Pavlos Pavlidis, Epameinondas Vasilikos, Doxakis Anestakis, Nikolaos Raikos
"Complex suicide" is a term referring to the suicidal pattern in which more than one suicidal method is applied in the purpose of inducing death. The present paper aims to review complex suicide literature shortly and investigate an unusual planned complex suicide incident, the rarity of which is attributable to the combination of suicide methods as well as the type and quantity of substances applied to induce poisoning. A 33-year-old man with a history of psychotic depression was found dead in his bedroom lying within a large quantity of blood...
2017: Soudní Lékarství
https://www.readbyqxmd.com/read/29226970/a-dominant-interfering-camta3-mutation-compromises-primary-transcriptional-outputs-mediated-by-both-cell-surface-and-intracellular-immune-receptors-in-arabidopsis-thaliana
#18
Florence Jacob, Barbara Kracher, Akira Mine, Carolin Seyfferth, Servane Blanvillain-Baufumé, Jane E Parker, Kenichi Tsuda, Paul Schulze-Lefert, Takaki Maekawa
Pattern recognition receptors (PRRs) and nucleotide-binding domain and leucine-rich repeat (LRR)-containing proteins (NLRs) initiate pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), respectively, each associated with the activation of an overlapping set of defence genes. The regulatory mechanism behind this convergence of PTI- and ETI-mediated defence gene induction remains elusive. We generated transgenic Arabidopsis plants that enable conditional NLR activation without pathogen infection to dissect NLR- and PRR-mediated transcriptional signals...
December 11, 2017: New Phytologist
https://www.readbyqxmd.com/read/29224048/comparison-between-whole-body-and-head-and-neck-neurovascular-coils-for-3-t-magnetic-resonance-proton-resonance-frequency-shift-thermography-guidance-in-the-head-and-neck-region
#19
Daniel Thomas Ginat, Gregory J Anthony, Gregory Christoforidis, Aytekin Oto, Leonard Dalag, Steffen Sammet
The purpose of this study is to compare the image quality of magnetic resonance (MR) treatment planning images and proton resonance frequency (PRF) shift thermography images and inform coil selection for MR-guided laser ablation of tumors in the head and neck region. Laser ablation was performed on an agar phantom and monitored via MR PRF shift thermography on a 3-T scanner, following acquisition of T1-weighted (T1W) planning images. PRF shift thermography images and T2-weighted (T2W) planning images were also performed in the neck region of five normal human volunteers...
December 9, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/29223826/modulation-effect-of-double-strand-dna-on-the-self-assembly-of-n-terminal-domain-of-euplotes-octocarinatus-centrin
#20
Wenlong Zhang, Enxian Shi, Yaqin Zhao, Binsheng Yang
Centrin is a member of the EF-hand super family of calcium-binding proteins, which can behave as a part of damage detector initiated the initiation of nucleotide excision repair (NER). Its self-assembly plays a causative role in fiber contraction associated with the cell division cycle and ciliogenesis. To explore the possible role of DNA in the process of centrin self-assembly, the aggregation properties of N-terminal domain of Euplotes octocarinatus centrin (N-EoCen) in the presence of DNA with or without metal ions are investigated...
December 5, 2017: Journal of Inorganic Biochemistry
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