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https://www.readbyqxmd.com/read/28425679/soft-interactions-and-volume-exclusion-by-polymeric-crowders-can-stabilize-or-destabilize-transient-structure-in-disordered-proteins-depending-on-polymer-concentration
#1
Farai I Rusinga, David D Weis
The effects of macromolecular crowding on the transient structure of intrinsically disordered proteins is not well-understood. Crowding by biological molecules inside cells could modulate transient structure and alter IDP function. Volume exclusion theory and observations of structured proteins suggest that IDP transient structure would be stabilized by macromolecular crowding. Amide hydrogen exchange (HX) of IDPs in highly concentrated polymer solutions would provide valuable insights into IDP transient structure under crowded conditions...
April 20, 2017: Proteins
https://www.readbyqxmd.com/read/28424572/functional-sensitivity-of-2d-simultaneous-multi-slice-echo-planar-imaging-effects-of-acceleration-on-g-factor-and-physiological-noise
#2
Nick Todd, Oliver Josephs, Peter Zeidman, Guillaume Flandin, Steen Moeller, Nikolaus Weiskopf
Accelerated data acquisition with simultaneous multi-slice (SMS) imaging for functional MRI studies leads to interacting and opposing effects that influence the sensitivity to blood oxygen level-dependent (BOLD) signal changes. Image signal to noise ratio (SNR) is decreased with higher SMS acceleration factors and shorter repetition times (TR) due to g-factor noise penalties and saturation of longitudinal magnetization. However, the lower image SNR is counteracted by greater statistical power from more samples per unit time and a higher temporal Nyquist frequency that allows for better removal of spurious non-BOLD high frequency signal content...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28424284/a-novel-type-of-polyhedral-viruses-infecting-hyperthermophilic-archaea
#3
Ying Liu, Sonoko Ishino, Yoshizumi Ishino, Gérard Pehau-Arnaudet, Mart Krupovic, David Prangishvili
Encapsidation of genetic material into polyhedral particles is one of the most common structural solutions employed by viruses infecting hosts in all three domains of life. Here, we describe a new virus of hyperthermophilic archaea, Sulfolobus polyhedral virus 1 (SPV1), which condenses its circular double-stranded DNA genome in a manner not previously observed for other known virus. The genome complexed with virion proteins is wound up sinusoidally into a spherical coil which is surrounded by an envelope and further encased by an outer polyhedral capsid apparently composed of the 20 kDa virion protein...
April 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28422092/hierarchical-assembly-of-centriole-subdistal-appendages-via-centrosome-binding-proteins-ccdc120-and-ccdc68
#4
Ning Huang, Yuqing Xia, Donghui Zhang, Song Wang, Yitian Bao, Runsheng He, Junlin Teng, Jianguo Chen
In animal cells, the centrosome is the main microtubule-organizing centre where microtubules are nucleated and anchored. The centriole subdistal appendages (SDAs) are the key structures that anchor microtubules in interphase cells, but the composition and assembly mechanisms of SDAs are not well understood. Here, we reveal that centrosome-binding proteins, coiled-coil domain containing (CCDC) 120 and CCDC68 are two novel SDA components required for hierarchical SDA assembly in human cells. CCDC120 is anchored to SDAs by ODF2 and recruits CEP170 and Ninein to the centrosome through different coiled-coil domains at its N terminus...
April 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28421683/a-numerical-investigation-on-the-effect-of-rf-coil-feed-variability-on-global-and-local-electromagnetic-field-exposure-in-human-body-models-at-64-mhz
#5
Elena Lucano, Micaela Liberti, Tom Lloyd, Francesca Apollonio, Steve Wedan, Wolfgang Kainz, Leonardo M Angelone
PURPOSE: This study aims to investigate how the positions of the feeding sources of the transmit radiofrequency (RF) coil, field orientation direction with respect to the patient, and patient dimensions affect the global and local electromagnetic exposure in human body models. METHODS: Three RF coil models were implemented, namely a specific two-source (S2) feed and two multisource feed configurations: generic 32-source (G32) and hybrid 16-source (H16). Thirty-two feeding conditions were studied for the S2, whereas two were studied for the G32 and H16...
April 18, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28421215/control-of-supramolecular-nanoassemblies-by-tuning-the-interactions-of-bent-shaped-rod-coil-molecules
#6
Shengnan You, Keli Zhong, Long Yi Jin
Rod-coil molecules 1a, 1b and 2a, 2b, consisting of biphenyl and phenyl units connected by an acetylene bond as the rod segment and oligo(ethylene glycol) (OEG) as the coil segment, were synthesized and characterized. Molecules 1a and 1b incorporate a butoxy group at the apex of their bent-shaped rigid building blocks, while both 1b and 2b contain a lateral methyl group between the rod and coil segments. The self-assembling behavior of these molecules was investigated using DSC, SAXS, CD, AFM, and TEM in bulk and aqueous solutions...
April 19, 2017: Soft Matter
https://www.readbyqxmd.com/read/28419533/a-new-approach-for-radiosynoviorthesis-a-dose-optimized-planning-method-based-on-monte-carlo-simulation-and-synovial-measurement-using-3d-slicer-and-mri
#7
Mirta Bárbara Torres Berdeguez, Sylvia Thomas, Patricia Rafful, Tiago Arruda Sanchez, Susie Medeiros Oliveira Ramos, Marta Souza Albernaz, Lidia Vasconcellos de Sá, Sergio Augusto Lopes de Souza, Felix Mas Milian, Ademir Xavier da Silva
PURPOSE: Recently, there has been a growing interest in a methodology for dose planning in radiosynoviorthesis to substitute fixed activity. Clinical practice based on fixed activity frequently does not embrace radiopharmaceutical dose optimization in patients. The aim of this paper is to propose and discuss a dose planning methodology considering the radiological findings of interest obtained by three-dimensional magnetic resonance imaging combined with Monte Carlo simulation in radiosynoviorthesis treatment applied to hemophilic arthropathy...
April 17, 2017: Medical Physics
https://www.readbyqxmd.com/read/28419294/preliminary-study-of-tirofiban-infusion-in-coil-embolization-of-ruptured-intracranial-aneurysms
#8
Chang Hyo Yoon, Ho-Won Lee, Young Soo Kim, Sang Won Lee, Jeong A Yeom, Jieun Roh, Seung Kug Baik
BACKGROUND: There have been some reports on the use of intra-arterial tirofiban in ruptured intracranial aneurysms, but few studies have reported on the use of 24 h of intravenous tirofiban infusion in patients with subarachnoid hemorrhage. OBJECTIVE: To present our experience with intravenous tirofiban infusion, in the form of a monotherapy as well as in addition to intra-arterial tirofiban, as a prophylactic, and as a rescue management for thrombus in patients who have undergone embolization with coils for ruptured intracranial aneurysms...
April 17, 2017: Neurosurgery
https://www.readbyqxmd.com/read/28419217/shock-efficacy-of-single-and-dual-coil-electrodes-new-insights-from-the-nordic-icd-trial
#9
Dietmar Bänsch, Hendrik Bonnemeier, Johan Brandt, Frank Bode, Jesper Hastrup Svendsen, Oliver Ritter, Johannes Aring, Klaus-Jürgen Gutleben, Ralph Schneider, Angelika Felk, Tino Hauser, Anika Buchholz, Gerhard Hindricks, Karl Wegscheider
Aims: Dual coil (DC) electrodes are preferred to single coil (SC) electrodes because of an assumed higher shock efficacy. However, DC-electrodes may be associated with an increased difficulty and risk of lead extraction. We aimed to compare SC- and DC-electrodes with respect to the first shock efficacy (FSE) after implantable cardioverter defibrillator (ICD) implantation. Methods and results: One thousand and seventy-seven patients of the NORDIC ICD trial were randomly assigned to first time ICD implantation with or without defibrillation (DF) testing...
April 13, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28417637/a-synergistic-enhancement-of-microwave-absorption-using-hybridized-polyaniline-helical-cnts-with-dual-chirality
#10
Xin Tian, Fanbin Meng, Fanchen Meng, Xiangnan Chen, Yifan Guo, Ying Wang, Wenjun Zhu, Zuowan Zhou
We designed a dual chirality hierarchical structure to achieve a synergistically enhanced effect in microwave absorption via the hybridization of nanomaterials. Herein, polyaniline (PANi) nanorods with tunable chirality are grown on helical carbon nanotubes (HCNTs), a typical nanoscale chiral structure, through in situ polymerization. The experimental results show that the hierarchical hybrids (PANi@HCNTs) exhibit distinctly dual chirality and display a significant enhancement in electromagnetic losses compared to either pure PANi or HCNTs...
April 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28417489/management-of-septic-emboli-in-patients-with-infectious-endocarditis
#11
Seyed Hossein Aalaei-Andabili, Tomas Martin, Phillip Hess, Brian Hoh, Meshka Anderson, Charles T Klodell, Thomas M Beaver
BACKGROUND AND AIM: Septic emboli (SE) associated with infectious endocarditis (IE) can result in splenic abscesses and infectious intracranial aneurysms (IIA). We investigated the impact of SE on patient outcomes following surgery for IE. METHOD: From January-2000 to October-2015, all patients with surgical IE (n = 437) were evaluated for incidence and management of SE. RESULTS: Overall SE was found in 46/437 (10.52%) patients (n = 17 spleen, 13 brain, and 16 both)...
April 17, 2017: Journal of Cardiac Surgery
https://www.readbyqxmd.com/read/28417323/polymer-chain-collapse-induced-by-many-body-dipole-correlations
#12
Yu A Budkov, N N Kalikin, A L Kolesnikov
We present a simple analytical theory of a flexible polymer chain dissolved in a good solvent, carrying permanent freely oriented dipoles on the monomers. We take into account the dipole correlations within the random phase approximation (RPA), as well as a dielectric heterogeneity in the internal polymer volume relative to the bulk solution. We demonstrate that the dipole correlations of monomers can be taken into account as pairwise ones only when the polymer chain is in a coil conformation. In this case the dipole correlations manifest themselves through the Keesom interactions of the permanent dipoles...
April 2017: European Physical Journal. E, Soft Matter
https://www.readbyqxmd.com/read/28417161/ift54-regulates-ift20-stability-but-is-not-essential-for-tubulin-transport-during-ciliogenesis
#13
Xin Zhu, Yinwen Liang, Feng Gao, Junmin Pan
Intraflagellar transport (IFT) is required for ciliogenesis by ferrying ciliary components using IFT complexes as cargo adaptors. IFT54 is a component of the IFT-B complex and is also associated with cytoplasmic microtubules (MTs). Loss of IFT54 impairs cilia assembly as well as cytoplasmic MT dynamics. The N-terminal calponin homology (CH) domain of IFT54 interacts with tubulins/MTs and has been proposed to transport tubulin during ciliogenesis, whereas the C-terminal coiled-coil (CC) domain binds IFT20. However, the precise function of these domains in vivo is not well understood...
April 17, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28416410/progressive-occlusion-and-recanalization-after-endovascular-treatment-for-287-unruptured-small-aneurysms-5mm-a-single-center-6-year-experience
#14
Xin Feng, Luyao Wang, Erkang Guo, Baorui Zhang, Zenghui Qian, Peng Liu, Xiaolong Wen, Wenjuan Xu, Youxiang Li, Chuhan Jiang, Zhongxue Wu, Aihua Liu
OBJECT: We aimed to investigate the effect of coiling for small unruptured intracranial aneurysms(UIAs)﹤5mm) on progressive occlusion and recanalization, and the dubious factors related to progressive occlusion and recanalization among UIAs without complete occlusion. METHODS: A total of 264 patients with 287 small UIAs were coiled in our institute between June 2009 and December 2014. All UIAs enrolled were divided into small (3-5mm) and very small (<3mm) groups, and UIAs without initial complete occlusion were divided into progressive, stable and recanalization groups...
April 14, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28416090/clipping-in-awake-surgery-as-end-stage-in-a-complex-internal-carotid-artery-aneurysm-after-failure-of-multimodal-endovascular-and-extracranial-intracranial-bypass-treatment
#15
Delia Cannizzaro, Simone Peschillo, Cristina Mancarella, Biagia La Pira, Emanuela Rastelli, Emiliano Passacantilli, Antonio Santoro
BACKGROUND: Intracranial carotid artery aneurysm can be treated via microsurgical or endovascular techniques. The optimal planning is the result of the careful patient selection through clinical, anatomic, and angiographic analysis. CLINICAL PRESENTATION: We present a case of ruptured internal carotid artery (ICA) aneurysm that became a complex aneurysm after failure of multi-endovascular and surgery treatment. We describe complete trapping in awake craniotomy after failure of coiling, stenting, and bypassing...
April 13, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28415199/identifying-polymer-states-by-machine-learning
#16
Qianshi Wei, Roger G Melko, Jeff Z Y Chen
The ability of a feed-forward neural network to learn and classify different states of polymer configurations is systematically explored. Performing numerical experiments, we find that a simple network model can, after adequate training, recognize multiple structures, including gaslike coil, liquidlike globular, and crystalline anti-Mackay and Mackay structures. The network can be trained to identify the transition points between various states, which compare well with those identified by independent specific-heat calculations...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28414975/fullerene-doped-polyaniline-as-new-redox-nanoprobe-and-catalyst-in-electrochemical-aptasensor-for-ultrasensitive-detection-of-mycobacterium-tuberculosis-mpt64-antigen-in-human-serum
#17
Lijuan Bai, Yuhan Chen, Yan Bai, Yongjie Chen, Jing Zhou, Ailong Huang
Tuberculosis caused by Mycobacterium tuberculosis (MTB) is still a major threat to global public health. However, the existing methods for MTB detection are usually complicated and time consuming with unsatisfactory sensitivity and specificity. In this work, a relatively simple and ultrasensitive electrochemical aptasensor based on novel signal generation and amplification was constructed for the determination of MTB antigen MPT64. The coil-like fullerene (C60)-doped polyaniline (C60-PAn) nanohybrids with large surface area, abundant active groups and excellent electric performance were synthesized and used both as new redox nanoprobe and catalyst for the generation and amplification of electrochemical signal for the first time...
April 12, 2017: Biomaterials
https://www.readbyqxmd.com/read/28414964/insights-into-the-short-term-effects-of-ceo2-nanoparticles-on-sludge-dewatering-and-related-mechanism
#18
Guoxiang You, Peifang Wang, Jun Hou, Chao Wang, Yi Xu, Lingzhan Miao, Bowen Lv, Yangyang Yang, Zhilin Liu, Fei Zhang
As CeO2 nanoparticles (NPs) are used in many applications, the released particles eventually enter wastewater treatment plants, influencing sludge dewatering and increasing the disposal costs. We conducted tests to gain insight into the sludge dewatering performance and the potential mechanism related to the distribution and production of extracellular polymeric substances (EPS). In addition, we conducted single-factor tests with six concentrations of CeO2 NPs in sequencing batch reactors containing matured activated sludge...
April 8, 2017: Water Research
https://www.readbyqxmd.com/read/28414871/effect-of-platelet-rich-plasma-on-orthodontic-tooth-movement-in-dogs
#19
A Rashid, F A ElSharaby, E M Nassef, S Mehanni, Y A Mostafa
OBJECTIVES: To evaluate the effect of platelet-rich plasma (PRP) on the rate of orthodontic tooth movement. METHODS: The sample comprised six skeletally mature male mongrel dogs. The maxillary second premolar in each dog was extracted bilaterally. PRP was prepared and injected around the first premolar in one randomly selected maxillary quadrant while the other quadrant served as the control. Coil springs (150 g) were used to distalize the first premolars for 63 days using TAD as anchorage...
May 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28413881/molecular-mechanism-on-stabilizing-huntingtin-n17-helical-structure-in-micelle-environment
#20
Leili Zhang, Hongsuk Kang, Francisco X Vázquez, Leticia Marisel Toledo-Sherman, Binquan Luan, Ruhong Zhou
Huntington's disease is a deadly neurodegenerative disease caused by the fibrilization of huntingtin (HTT) exon-1 protein mutants. Despite extensive efforts over the past decade, much remains unknown about the structures of (mutant) HTT exon-1 and their enigmatic roles in aggregation. Particularly, whether the first 17 residues in the N-terminal (HTT-N17) adopt a helical or a coiled structure remains unclear. Here, with the rigorous study of molecular dynamics simulations, we explored the most possible structures of HTT-N17 in both dodecylphosphocholine (DPC) micelles and aqueous solution, using three commonly applied force fields (OPLS-AA/L, CHARMM36 and AMBER99sb*-ILDNP) to examine the underlying molecular mechanism and rule out the potential artifacts...
April 17, 2017: Journal of Physical Chemistry. B
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