keyword
MENU ▼
Read by QxMD icon Read
search

phosphatidylserine

keyword
https://www.readbyqxmd.com/read/28535537/triggering-of-suicidal-erythrocyte-death-by-exemestane
#1
Abdulla Al Mamun Bhuyan, Rosi Bissinger, Hang Cao, Florian Lang
BACKGROUND/AIMS: The steroidal aromatase inactivator exemestane blocks estrogen biosynthesis and is thus employed for the prevention and treatment of breast cancer. Exemestane is in part effective by stimulation of suicidal cell death or apoptosis. Side effects of exemestane treatment include anemia. At least in theory, exemestane induced anemia could be secondary to stimulation of suicidal erythrocyte death or eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface...
May 11, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28530650/multi-virion-infectious-units-arise-from-free-viral-particles-in-an-enveloped-virus
#2
José M Cuevas, María Durán-Moreno, Rafael Sanjuán
Many animal viruses are enveloped in a lipid bilayer taken up from cellular membranes. Because viral surface proteins bind to these membranes to initiate infection, we hypothesized that free virions may also be capable of interacting with the envelopes of other virions extracellularly. Here, we demonstrate this hypothesis in the vesicular stomatitis virus (VSV), a prototypic negative-strand RNA virus composed of an internal ribonucleocapsid, a matrix protein and an external envelope(1). Using microscopy, dynamic light scattering, differential centrifugation and flow cytometry, we show that free viral particles can spontaneously aggregate into multi-virion infectious units...
May 22, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28528260/hexavalent-chromium-induced-oxidative-stress-and-apoptosis-in-pycnoporus-sanguineus
#3
Mi Feng, Hua Yin, Hui Peng, Zehua Liu, Guining Lu, Zhi Dang
White rot fungi have been proved to be a promising option for the removal of heavy metals, understanding their toxic response to heavy metals is conducive to developing and popularizing fungi-based remediation technologies so as to lessen the hazard of heavy metals. In this study, Cr(VI)-induced oxidative stress and apoptosis in Pycnoporus sanguineus, a species of white rot fungi were investigated. The results suggested that high level of Cr(VI) promoted the formation of ROS, including H2O2, O2(•-) and ·OH...
May 18, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28524456/endothelial-microparticles-released-by-activated-protein-c-protect-beta-cells-through-epcr-par1-and-annexin-a1-fpr2-pathways-in-islets
#4
Guillaume Kreutter, Mohamad Kassem, Ali El Habhab, Philippe Baltzinger, Malak Abbas, Julie Boisrame-Helms, Lamia Amoura, Jean Peluso, Blandine Yver, Zobairi Fatiha, Geneviève Ubeaud-Sequier, Laurence Kessler, Florence Toti
Islet transplantation is associated with early ischaemia/reperfusion, localized coagulation and redox-sensitive endothelial dysfunction. In animal models, islet cytoprotection by activated protein C (aPC) restores islet vascularization and protects graft function, suggesting that aPC triggers various lineages. aPC also prompts the release of endothelial MP that bear EPCR, its specific receptor. Microparticles (MP) are plasma membrane procoagulant vesicles, surrogate markers of stress and cellular effectors...
May 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28522586/antitumor-synergism-and-enhanced-survival-with-a-tumor-vasculature-targeted-enzyme-prodrug-system-rapamycin-and-cyclophosphamide
#5
John J Krais, Needa Virani, Partick H McKernan, Quang Nguyen, Kar-Ming Fung, Vassilios I Sikavitsas, Carla D Kurkjian, Roger G Harrison
Mutant cystathionine gamma-lyase was targeted to phosphatidylserine exposed on tumor vasculature through fusion with annexin A1 or annexin A5.  Cystathionine gamma-lyase E58N, R118L, and E338N mutations impart non-native methionine gamma-lyase activity, resulting in tumor-localized generation of highly toxic methylselenol upon systemic administration of non-toxic selenomethionine.  The described therapeutic system circumvents systemic toxicity issues using a novel drug delivery/generation approach and avoids the administration of non-native proteins and/or DNA required with other enzyme prodrug systems...
May 18, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28514834/antitumor-activity-of-brazilian-red-propolis-fractions-against-hep-2-cancer-cell-line
#6
Caroline Olivieri da Silva Frozza, Denis Amilton Santos, Luciane Corbellini Rufatto, Luciane Minetto, Fernando Joel Scariot, Sergio Echeverrigaray, Claus Tröger Pich, Sidnei Moura, Francine Ferreira Padilha, Sibele Borsuk, Lucielli Savegnago, Tiago Collares, Fabiana Kömmling Seixas, Odir Dellagostin, Mariana Roesch-Ely, João Antonio Pêgas Henriques
Continuous increases in the rates of tumor diseases have highlighted the need for identification of novel and inexpensive antitumor agents from natural sources. In this study, we investigated the effects of enriched fraction from hydroalcoholic Brazilian red propolis extract against Hep-2 cancer cell line. Initially 201 fractions were arranged in 12 groups according to their chromatographic characteristics (A-L). After an in vitro cell viability screening, J and L were further selected as promising enriched fractions for this study...
May 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28512733/a-lipidomic-cell-based-assay-for-studying-drug-induced-phospholipidosis-and-steatosis
#7
Juan Carlos García-Cañaveras, Manuel David Peris-Díaz, M Isabel Alcoriza, Manuela Cerdán-Calero, M Teresa Donato, Agustín Lahoz
Phospholipidosis and steatosis are two toxic effects, which course with overaccumulation of different classes of lipids in the liver. MS-based lipidomics has become a powerful tool for the comprehensive determination of lipids. LC-MS lipid profiling of HepG2 cells is proposed as an in vitro assay to study and anticipate phospholipidosis and steatosis. Cells with and without pre-incubation with a mixture of free fatty acids (FFA) (i.e., oleic and palmitic) were exposed to a set of well-known steatogenic and phospholipidogenic compounds...
May 17, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28509615/mining-of-a-phospholipase-d-and-its-application-in-enzymatic-preparation-of-phosphatidylserine
#8
Wen-Bin Zhou, Jin-Song Gong, Hai-Juan Hou, Heng Li, Zhen-Ming Lu, Hong-Yu Xu, Zheng-Hong Xu, Jin-Song Shi
Phosphatidylserine (PS) is useful as the additive in industries for memory improvement, mood enhancement and drug delivery. Conventionally, PS was extracted from soybeans, vegetable oils, egg yolk, and biomass; however, their low availability and high extraction cost were limiting factors. Phospholipase D (PLD) is a promising tool for enzymatic synthesis of PS due to its transphosphatidylation activity. In this contribution, a new and uncharacterized PLD was first obtained from GenBank database via genome mining strategy...
May 16, 2017: Bioengineered
https://www.readbyqxmd.com/read/28505535/thiosemicarbazones-and-4-thiazolidinones-indole-based-derivatives-synthesis-evaluation-of-antiproliferative-activity-cell-death-mechanisms-and-topoisomerase-inhibition-assay
#9
Jamerson Ferreira de Oliveira, Talitha Santos Lima, Débora Barbosa Vendramini-Costa, Sybelle Christianne Batista de Lacerda Pedrosa, Elizabeth Almeida Lafayette, Rosali Maria Ferreira da Silva, Sinara Monica Vitalino de Almeida, Ricardo Olímpio de Moura, Ana Lúcia Tasca Gois Ruiz, João Ernesto de Carvalho, Maria do Carmo Alves de Lima
In this study, we report the synthesis and structural characterization of a series of thiosemicarbazone and 4-thiazolidinones derivatives, as well as their in vitro antiproliferative activity against eight human tumor cell lines. For the most potent compound further studies were performed evaluating cell death induction, cell cycle profile, ctDNA interaction and topoisomerase IIα inhibition. A synthetic three-step route was established for compounds (2a-e and 3a-d) with yields ranging from 32 to 95%. Regarding antiproliferative activity, compounds 2a-e and 3a-d showed mean GI50 values ranging between 1...
May 8, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28495929/tissue-factor-prothrombotic-activity-is-regulated-by-integrin-arf6-trafficking
#10
Andrea S Rothmeier, Patrizia Marchese, Florian Langer, Yuichi Kamikubo, Florence Schaffner, Joseph Cantor, Mark H Ginsberg, Zaverio M Ruggeri, Wolfram Ruf
OBJECTIVE: Coagulation initiation by tissue factor (TF) is regulated by cellular inhibitors, cell surface availability of procoagulant phosphatidylserine, and thiol-disulfide exchange. How these mechanisms contribute to keeping TF in a noncoagulant state and to generating prothrombotic TF remain incompletely understood. APPROACH AND RESULTS: Here, we study the activation of TF in primary macrophages by a combination of pharmacological, genetic, and biochemical approaches...
May 11, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28495859/alpha-synuclein-may-cross-bridge-v-snare-and-acidic-phospholipids-to-facilitate-snare-dependent-vesicle-docking
#11
Xiaochu Lou, Jaewook Kim, Brenden J Hawk, Yeon-Kyun Shin
Misfolded α-synuclein/A-syn is widely recognized as primal cause of neurodegenerative diseases including Parkinson's and dementia with Lewy bodies. The normal cellular function of A-syn has however been elusive. There is evidence that A-syn plays a variety of roles in the exocytotic pathway in the neuron, but the underlying molecular mechanisms are unclear. A-syn has been known to interact with negatively charged phospholipids and with vesicle SNARE protein VAMP2 as well. Using single vesicle-vesicle docking/fusion assays, we find that A-syn promotes SNARE-dependent vesicles docking significantly at 2...
May 11, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28489923/differences-of-microparticle-patterns-between-sickle-cell-anemia-and-hemoglobin-sc-patients
#12
Yohann Garnier, Séverine Ferdinand, Maryse Etienne-Julan, Gisèle Elana, Marie Petras, Lydia Doumdo, Benoit Tressières, Marie-Laure Lalanne-Mistrih, Marie-Dominique Hardy-Dessources, Philippe Connes, Marc Romana
Sickle cell anemia (SCA) and hemoglobin SC (HbSC) disease are the two most common forms of sickle cell disease (SCD), a frequent hemoglobinopathy which exhibits a highly variable clinical course. Although high levels of microparticles (MPs) have been consistently reported in SCA and evidence of their harmful impact on the SCA complication occurrences have been provided, no data on MP pattern in HbSC patients has been reported so far. In this study, we determined and compared the MP patterns of 84 HbSC and 96 SCA children, all at steady-state, using flow cytometry...
2017: PloS One
https://www.readbyqxmd.com/read/28488761/apoptotic-sperm-biomarkers-and-the-correlation-between-conventional-sperm-parameters-and-clinical-characteristics
#13
R Hichri, H Amor, M Khammari, M Harzallah, S El Fekih, A Saad, M Ajina, H Ben Ali
The principal aim of this retrospective study was to examine the relationship between sperm apoptotic biomarkers and the patient's biclinical characteristics, the conventional sperm parameters and the results of assisted reproductive technology. Sperm analysis, activated caspases, annexin V staining for phosphatidylserine (PS) externalisation and labelling assay for DNA fragmentation were assessed in 122 males of infertile couples. Fifty-seven couples were allocated to the natural conception group, and 65 couples underwent IVF or ICSI...
May 10, 2017: Andrologia
https://www.readbyqxmd.com/read/28484605/how-do-red-blood-cells-know-when-to-die
#14
Clemente Fernandez Arias, Cristina Fernandez Arias
Human red blood cells (RBCs) are normally phagocytized by macrophages of splenic and hepatic sinusoids at 120 days of age. The destruction of RBCs is ultimately controlled by antagonist effects of phosphatidylserine (PS) and CD47 on the phagocytic activity of macrophages. In this work, we introduce a conceptual model that explains RBC lifespan as a consequence of the dynamics of these molecules. Specifically, we suggest that PS and CD47 define a molecular algorithm that sets the timing of RBC phagocytosis. We show that significant changes in RBC lifespan described in the literature can be explained as alternative outcomes of this algorithm when it is executed in different conditions of oxygen availability...
April 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28484043/the-long-hunt-for-pssr-looking-for-a-phospholipid-synthesis-transcriptional-regulator-finding-the-ribosome
#15
J Bartoli, L My, Lucid Belmudes, Yohann Couté, J P Viala, E Bouveret
The phospholipid (PL) composition of bacterial membranes varies as a function of growth rate and in response to changes in the environment. While growth adaptation can be explained by biochemical feedbacks in the PL synthesis pathway, recent transcriptome studies have revealed that the expression of PL synthesis genes can also be tuned in response to various stresses. We previously showed that the BasRS two-component pathway controls expression of the diacylglycerol kinase gene dgkA in Escherichia coli (Wahl et al, Mol...
May 8, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28483690/characterization-of-the-lipid-envelope-of-exosome-encapsulated-hev-particles-protected-from-the-immune-response
#16
Sabine Chapuy-Regaud, Martine Dubois, Célia Plisson-Chastang, Tiffany Bonnefois, Sébastien Lhomme, Justine Bertrand-Michel, Bruno You, Steve Simoneau, Pierre-Emmanuel Gleizes, Benoît Flan, Florence Abravanel, Jacques Izopet
The hepatitis E virus (HEV) is the most common cause of acute hepatitis worldwide. Although HEV is a small, naked RNA virus, HEV particles become associated with lipids in the blood of infected patients and in the supernatant of culture systems. The egress of these particles from cells implies the exocytosis pathway but the question of the role of the resulting HEV RNA containing exosomes and the nature of the lipids they contain has not been fully addressed. We determined the lipid proportions of exosomes from uninfected and HEV-infected cells and their role in HEV spreading...
May 5, 2017: Biochimie
https://www.readbyqxmd.com/read/28482935/nerolidol-the-main-constituent-of-piper-aduncum-essential-oil-has-anti-leishmania-braziliensis-activity
#17
Ligia Fernanda Ceole, Maria DAS Graças Cardoso, Maurilio José Soares
Leishmania (Viannia) braziliensis is a protozoan that causes mucocutaneous leishmaniasis, which is an infectious disease that affects more than 12 million people worldwide. The available treatment is limited, has side-effects or is inefficient. In a search for alternative compounds of natural origin, we tested the microbicidal activity of Piper aduncum essential oil (PaEO) on this parasite. Our data showed that PaEO had an inhibitory effect on the growth of L. braziliensis promastigotes with an IC50/24 h=77·9 µg mL-1...
May 9, 2017: Parasitology
https://www.readbyqxmd.com/read/28475026/solibacillus-kalamii-sp-nov-isolated-from-a-high-efficiency-particulate-arrestance-filter-system-used-in-the-international-space-station
#18
Aleksandra Checinska Sielaff, Rajendran Mathan Kumar, Deepika Pal, Shanmugam Mayilraj, Kasthuri Venkateswaran
A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterial strain, designated ISSFR-015T, was isolated from a high-efficiency particulate arrestance filter in the International Space Station and was characterized by polyphasic taxonomy. A comparative analysis of the 16S rRNA gene sequence (1494 bp) of strain ISSFR-015T showed highest similarity to Solibacillus isronensis B3W22T (98.9 %), followed by Solibacillus silvestris HR3-23T (98.6 %) and Bacillus cecembensis PN5T (96.7 %). DNA-DNA hybridization analysis revealed that the DNA relatedness values of strain ISSFR-015T with other closely related species were in the range of 41-47 % [S...
April 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28473884/characterisation-of-adipocyte-derived-extracellular-vesicle-subtypes-identifies-distinct-protein-and-lipid-signatures-for-large-and-small-extracellular-vesicles
#19
Maëva Durcin, Audrey Fleury, Emiliane Taillebois, Grégory Hilairet, Zuzana Krupova, Céline Henry, Sandrine Truchet, Martin Trötzmüller, Harald Köfeler, Guillaume Mabilleau, Olivier Hue, Ramaroson Andriantsitohaina, Patrice Martin, Soazig Le Lay
Extracellular vesicles (EVs) are biological vectors that can modulate the metabolism of target cells by conveying signalling proteins and genomic material. The level of EVs in plasma is significantly increased in cardiometabolic diseases associated with obesity, suggesting their possible participation in the development of metabolic dysfunction. With regard to the poor definition of adipocyte-derived EVs, the purpose of this study was to characterise both qualitatively and quantitatively EVs subpopulations secreted by fat cells...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28473294/involvement-of-a-putative-substrate-binding-site-in-the-biogenesis-and-assembly-of-phosphatidylserine-decarboxylase-1-from-saccharomyces-cerevisiae
#20
Francesca Di Bartolomeo, Kim Nguyen Doan, Karin Athenstaedt, Thomas Becker, Günther Daum
In the yeast Saccharomyces cerevisiae, the mitochondrial phosphatidylserine decarboxylase 1 (Psd1p) produces the largest amount of cellular phosphatidylethanolamine (PE). Psd1p is synthesized as a larger precursor on cytosolic ribosomes and then imported into mitochondria in a three-step processing event leading to the formation of an α-subunit and a β-subunit. The α-subunit harbors a highly conserved motif, which was proposed to be involved in phosphatidylserine (PS) binding. Here, we present a molecular analysis of this consensus motif for the function of Psd1p by using Psd1p variants bearing either deletions or point mutations in this region...
May 1, 2017: Biochimica et Biophysica Acta
keyword
keyword
55951
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"