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phosphatidylserine

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https://www.readbyqxmd.com/read/29334397/recombinant-phosphatidylserine-binding-nanobodies-for-targeting-of-extracellular-vesicles-to-tumor-cells-a-plug-and-play-approach
#1
Sander A A Kooijmans, Jerney J J M Gitz-Francois, Raymond M Schiffelers, Pieter Vader
Extracellular vesicles (EVs) are increasingly being recognized as candidate drug delivery systems due to their ability to functionally transfer biological cargo between cells. However, manipulation of targeting properties of EVs through engineering of the producer cells can be challenging and time-consuming. As a novel approach to confer tumor targeting properties to isolated EVs, we generated recombinant fusion proteins of nanobodies against the epidermal growth factor receptor (EGFR) fused to phosphatidylserine (PS)-binding domains of lactadherin (C1C2)...
January 15, 2018: Nanoscale
https://www.readbyqxmd.com/read/29332224/phosphatidylserine-exposing-blood-and-endothelial-cells-contribute-to-the-hypercoagulable-state-in-essential-thrombocythemia-patients
#2
Dongxia Tong, Muxin Yu, Li Guo, Tao Li, Jihe Li, Valerie A Novakovic, Zengxiang Dong, Ye Tian, Junjie Kou, Yayan Bi, Jinghua Wang, Jin Zhou, Jialan Shi
The mechanisms of thrombogenicity in essential thrombocythemia (ET) are complex and not well defined. Our objective was to explore whether phosphatidylserine (PS) exposure on blood cells and endothelial cells (ECs) can account for the increased thrombosis and distinct thrombotic risks among mutational subtypes in ET. Using flow cytometry and confocal microscopy, we found that the levels of PS-exposing erythrocytes, platelets, leukocytes, and serum-cultured ECs were significantly higher in each ET group [JAK2, CALR, and triple-negative (TN) (all P < 0...
January 13, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29330691/bis-guanylhydrazones-as-efficient-anti-candida-compounds-through-dna-interaction
#3
Jelena Lazić, Vladimir Ajdačić, Sandra Vojnovic, Mario Zlatović, Marina Pekmezovic, Selene Mogavero, Igor Opsenica, Jasmina Nikodinovic-Runic
Candida spp. are leading causes of opportunistic mycoses, including life-threatening hospital-borne infections, and novel antifungals, preferably aiming targets that have not been used before, are constantly needed. Hydrazone- and guanidine-containing molecules have shown a wide range of biological activities, including recently described excellent antifungal properties. In this study, four bis-guanylhydrazone derivatives (BG1-4) were generated following a previously developed synthetic route. Anti-Candida (two C...
January 12, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29329503/chemical-imaging-of-aggressive-basal-cell-carcinoma-using-time-of-flight-secondary-ion-mass-spectrometry
#4
Marwa Munem, Oscar Zaar, Kelly Dimovska Nilsson, Noora Neittaanmäki, John Paoli, John S Fletcher
A set of basal cell carcinoma samples, removed by Mohs micrographic surgery and pathologically identified as having an aggressive subtype, have been analyzed using time-of-flight secondary ion mass spectrometry (SIMS). The SIMS analysis employed a gas cluster ion beam (GCIB) to increase the sensitivity of the technique for the detection of intact lipid species. The GCIB also allowed these intact molecular signals to be maintained while surface contamination and delocalized chemicals were removed from the upper tissue surface...
January 12, 2018: Biointerphases
https://www.readbyqxmd.com/read/29327917/peptide-based-photoelectrochemical-cytosensor-using-a-hollow-tio2-eg-znin2s4-co-sensitized-structure-for-ultrasensitive-detection-of-early-apoptotic-cells-and-drug-evaluation
#5
Rong Wu, Gao-Chao Fan, Li-Ping Jiang, Jun-Jie Zhu
The ability to rapidly detect apoptotic cells and accurately evaluate therapeutic effects is significant in cancer research. To address this target, a biocompatible, ultrasensitive photoelectrochemical (PEC) cytosensing platform was developed based on electrochemically reduced graphene (EG)/ZnIn2S4 co-sensitized TiO2 coupled with specific recognition between apoptotic cells and phosphatidylserine (PS)-binding peptide (PSBP). In this strategy, HL-60 cells were selected as a model, and C005, nilotinib and imatinib were selected as apoptosis inducers to show cytosensing performance...
January 12, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29324830/nano-pulse-stimulation-induces-immunogenic-cell-death-in-human-papillomavirus-transformed-tumors-and-initiates-an-adaptive-immune-response
#6
Joseph G Skeate, Diane M Da Silva, Elena Chavez-Juan, Snjezana Anand, Richard Nuccitelli, W Martin Kast
Nano-Pulse Stimulation (NPS) is a non-thermal pulsed electric field modality that has been shown to have cancer therapeutic effects. Here we applied NPS treatment to the human papillomavirus type 16 (HPV 16)-transformed C3.43 mouse tumor cell model and showed that it is effective at eliminating primary tumors through the induction of immunogenic cell death while subsequently increasing the number of tumor-infiltrating lymphocytes within the tumor microenvironment. In vitro NPS treatment of C3.43 cells resulted in a doubling of activated caspase 3/7 along with the translocation of phosphatidylserine (PS) to the outer leaflet of the plasma membrane, indicating programmed cell death activity...
2018: PloS One
https://www.readbyqxmd.com/read/29321615/functional-isolation-of-tumor-initiating-cells-using-microfluidic-based-migration-identifies-phosphatidylserine-decarboxylase-as-a-key-regulator
#7
Yu-Chih Chen, Brock Humphries, Riley Brien, Anne E Gibbons, Yu-Ting Chen, Tonela Qyli, Henry R Haley, Matthew E Pirone, Benjamin Chiang, Annie Xiao, Yu-Heng Cheng, Yi Luan, Zhixiong Zhang, Jason Cong, Kathryn E Luker, Gary D Luker, Euisik Yoon
Isolation of tumor-initiating cells currently relies on markers that do not reflect essential biologic functions of these cells. We proposed to overcome this limitation by isolating tumor-initiating cells based on enhanced migration, a function tightly linked to tumor-initiating potential through epithelial-to-mesenchymal transition (EMT). We developed a high-throughput microfluidic migration platform with automated cell tracking software and facile recovery of cells for downstream functional and genetic analyses...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321581/a-ruthenium-based-5-fluorouracil-complex-with-enhanced-cytotoxicity-and-apoptosis-induction-action-in-hct116-cells
#8
Valdenizia Rodrigues Silva, Rodrigo S Corrêa, Luciano de Souza Santos, Milena Botelho Pereira Soares, Alzir Azevedo Batista, Daniel Pereira Bezerra
Combination of multifunctionalities into one compound is a rational strategy in medicinal chemical design, and have often been used with metallodrug-based compounds. In the present study, we synthesized a novel ruthenium-based 5-fluorouracil complex [Ru(5-FU)(PPh3)2(bipy)]PF6 (PPh3 = triphenylphosphine; and bipy = 2,2'-bipyridine) with enhanced cytotoxicity in different cancer cells, and assessed its apoptosis induction action in human colon carcinoma HCT116 cells. The complex was characterized by infrared, cyclic voltammetry, molar conductance measurements, elemental analysis, NMR experiments and X-ray crystallographic analysis...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29318974/-understanding-the-role-of-hypoxia-inducible-factor-during-neurodegeneration-for-new-therapeutics-opportunities
#9
Amalia Merelli, Julio Cesar Garcia Rodriguez, Jaume Folch, Marcelo R Regueiro, Antoni Camins, Lazarowski Alberto
Neurodegeneration (NDG) is linked with the progressive loss of neural function with intellectual and/or motor impairment. Several diseases affecting older individuals, including Alzheimer's disease, Amyotrophic Lateral Sclerosis, Huntington's disease, Parkinson's disease, stroke, Multiple Sclerosis and many others, are the most relevant disorders associated with NDG. Since other pathologies such as refractory epilepsy, brain infections, or hereditary diseases such as "neurodegeneration with brain iron accumulation", also lead to chronic brain inflammation with loss of neural cells, NDG can be said to affect all ages...
January 10, 2018: Current Neuropharmacology
https://www.readbyqxmd.com/read/29318910/cloning-of-a-novel-lectin-from-artocarpus-lingnanensis-that-induces-apoptosis-in-human-b-lymphoma-cells
#10
Yu Luo, Xiaoqin Liu, Faquan Lin, Liejun Liao, Yong Deng, Linjie Zeng, Qiyan Zeng
We isolated a novel lectin (Artocarpus nitidus subsp. lingnanensis lectin, ALL) from Artocarpus nitidus subsp. lingnanensis and showed its mitogenic activities. In this study, we determined the amino acid sequence of ALL by cDNA sequencing. ALL cDNA (933 bp) contains a 657-bp open reading frame (ORF), which encodes a protein with 218 amino acids. ALL shares high sequence similarities with Jacalin and Morniga G and belongs to jacalin-related lectin family. We also examined the antitumor activity of ALL using Raji, a human B-lymphoma cell line...
January 10, 2018: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29317266/biochemical-characterization-of-unusual-cysteine-protease-of-p-falciparum-metacaspase-2-mca-2
#11
Vandana, Agam P Singh, Jitendra Singh, Ruby Sharma, Mymoona Akhter, Pradyumna K Mishra, Ajay K Saxena, Rajnikant Dixit, Brijesh Rathi, Anju Katyal, Kailash C Pandey
Earlier studies on Plasmodium apoptosis revealed the presence of proteases with caspases like- activity, which are known as "metacaspases". Although this family of cysteine proteases is structurally similar to caspases with Cys-His dyad but their evolutionary significance and functional relevance remains largely unknown. These proteases are considered to be an important target against malaria due to their absence in humans. In this report, we have biochemically characterized metacaspase-2 (PfMCA-2) of P.falciparum...
January 6, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29317198/rhodomyrtone-rom-is-a-membrane-active-compound
#12
Jongkon Saising, Minh Thu Nguyen, Thomas Härtner, Patrick Ebner, Abdulla Al Mamun Bhuyan, Anne Berscheid, Melanie Muehlenkamp, Sina Schäkermann, Nimerta Kumari, Martin E Maier, Supayang P Voravuthikunchai, Julia Bandow, Florian Lang, Heike Brötz-Oesterhelt, Friedrich Götz
Particularly in Asia medicinal plants with antimicrobial activity are used for therapeutic purpose. One such plant-derived antibiotic is rhodomyrtone (Rom) isolated from Rhodomyrtus tomentosa leaves. Rom shows high antibacterial activity against a wide range of Gram-positive bacteria, however, its mode of action is still unclear. Reporter gene assays and proteomic profiling experiments in Bacillus subtilis indicate that Rom does not address classical antibiotic targets like translation, transcription or DNA replication, but acts at the cytoplasmic membrane...
January 6, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29311487/acyl-chain-preference-in-foam-cell-formation-from-mouse-peritoneal-macrophages
#13
Yuko Fujiwara, Kotaro Hama, Makoto Tsukahara, Ryosuke Izumi-Tsuzuki, Toru Nagai, Mihoko Ohe-Yamada, Keizo Inoue, Kazuaki Yokoyama
Macrophage foam cells play critical roles in the initiation and development of atherosclerosis by synthesizing and accumulating cholesteryl ester (CE) in lipid droplets. However, in analyzing lipid metabolism in foam cell formation, studies have focused on the sterol group, and little research has been done on the acyl chains. Therefore, we adapted a model system using liposomes containing particular acyl chains and examined the effect of various acyl chains on foam cell formation. Of the phosphatidylserine (PS) liposomes tested containing PS, phosphatidylcholine, and cholesterol, we found that unsaturated (C18:1), but not saturated (C16:0 and C18:0), PS liposomes induced lipid droplet formation, indicating that foam cell formation depends on the nature of the acyl chain of the PS liposomes...
2018: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29305928/anti-proliferative-activity-of-biochanin-a-in-human-osteosarcoma-cells-via-mitochondrial-involved-apoptosis
#14
Yen-Nien Hsu, Huey-Wen Shyu, Tsui-Wen Hu, Jou-Pei Yeh, Ya-Wen Lin, Ling-Yi Lee, Yao-Tsung Yeh, Hong-Ying Dai, Daw-Shyong Perng, Shu-Hui Su, Yu-Hsuan Huang, Shu-Jem Su
Biochanin A is a major isoflavone in red clover and a potent chemopreventive agent against cancer. However, the effects of biochanin A on human osteosarcoma cells have never been clarified. This study investigated the anti-proliferative potential of biochanin A in osteosarcoma cells. The results indicate that biochanin A inhibited cell growth and colony formation in a dose-dependent manner with a minimal toxicity to normal cells. The combination of doxorubicin and biochanin A could synergistically inhibit osteosarcoma cell growth...
January 3, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29304470/protein-s-and-gas6-induce-efferocytosis-of-hiv-1-infected-cells
#15
Bernadette Anne Chua, Jamie Ann Ngo, Kathy Situ, Christina M Ramirez, Haruko Nakano, Kouki Morizono
Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular mechanisms are unknown. To elucidate the roles that efferocytosis of HIV-1-infected cells play in clearance of infected cells, we sought to identify molecules that mediate these processes. We found that protein S, present in human serum, and its homologue, Gas6, can mediate phagocytosis of HIV-1-infected cells by bridging receptor tyrosine kinase Mer, expressed on macrophages, to phosphatidylserine exposed on infected cells...
January 2, 2018: Virology
https://www.readbyqxmd.com/read/29302779/glia-to-neuron-transfer-of-mirnas-via-extracellular-vesicles-a-new-mechanism-underlying-inflammation-induced-synaptic-alterations
#16
Ilaria Prada, Martina Gabrielli, Elena Turola, Alessia Iorio, Giulia D'Arrigo, Roberta Parolisi, Mariacristina De Luca, Marco Pacifici, Mattia Bastoni, Marta Lombardi, Giuseppe Legname, Dan Cojoc, Annalisa Buffo, Roberto Furlan, Francesca Peruzzi, Claudia Verderio
Recent evidence indicates synaptic dysfunction as an early mechanism affected in neuroinflammatory diseases, such as multiple sclerosis, which are characterized by chronic microglia activation. However, the mode(s) of action of reactive microglia in causing synaptic defects are not fully understood. In this study, we show that inflammatory microglia produce extracellular vesicles (EVs) which are enriched in a set of miRNAs that regulate the expression of key synaptic proteins. Among them, miR-146a-5p, a microglia-specific miRNA not present in hippocampal neurons, controls the expression of presynaptic synaptotagmin1 (Syt1) and postsynaptic neuroligin1 (Nlg1), an adhesion protein which play a crucial role in dendritic spine formation and synaptic stability...
January 4, 2018: Acta Neuropathologica
https://www.readbyqxmd.com/read/29299846/larkinella-roseus-sp-nov-a-species-of-the-family-cytophagaceae-isolated-from-beach-soil
#17
Jae-Bong Lee, Sumin Hong, Seung-Yeol Lee, Su-Jin Park, Kyeung Il Park, Seok-Gwan Choi, Myung Kyum Kim, Leonid N Ten, Hee-Young Jung
The taxonomic position of bacterial strain, designated 15J16-1T3AT, recovered from a soil sample was established using a polyphasic approach. Phylogenic analysis based on the 16S rRNA gene sequence showed that strain 15J16-1T3AT belonged to the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to 'Larkinella harenae' 15J9-9 (95.9% similarity), Larkinella ripae 15J11-11T (95.6%), Larkinella bovis M2TB15T (94.7%), Larkinella arboricola Z0532T (93.9%), and Larkinella insperata LMG 22510T (93...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/29299119/identifying-mitotane-induced-mitochondria-associated-membranes-dysfunctions-metabolomic-and-lipidomic-approaches
#18
Ségolène Hescot, Larbi Amazit, Marie Lhomme, Simon Travers, Anais DuBow, Stephanie Battini, Geoffrey Boulate, Izzie Jacques Namer, Anne Lombes, Anatol Kontush, Alessio Imperiale, Eric Baudin, Marc Lombes
Mitotane (o,p'DDD), the most effective drug in adrenocortical carcinoma, concentrates into the mitochondria and impacts mitochondrial functions. To address the molecular mechanisms of mitotane action and to identify its potential target, metabolomic and lipidomic approaches as well as imaging analyses were employed in human adrenocortical H295R cells allowing identification of Mitochondria-Associated Membranes dysfunction as a critical impact of mitotane. Study of intracellular energetic metabolites by NMR spectroscopy showed that mitotane significantly decreased aspartate while concomitantly increased glutamate content in a time- and concentration-dependent manner...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29297991/application-of-lipidomics-to-reveal-differences-in-facial-skin-surface-lipids-between-males-and-females
#19
Le Cui, Cong-Fen He, Lin-Na Fan, Yan Jia
PURPOSE: To analyze and compare differences in facial skin surface lipids (SSL) between 18- to 25-year-old males and females. METHODS: Ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) technology was used to measure the facial SSL composition of 18- to 25-year-old males and females. Measurement results were combined with the orthogonal projections to latent structures discriminant analysis (OPLS-DA) model for analysis and comparisons, and differences in lipids with significance were selected...
January 3, 2018: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29297134/tamoxifen-induces-apoptotic-neutrophil-efferocytosis-in-horses
#20
C Olave, N Morales, B Uberti, C Henriquez, J Sarmiento, A Ortloff, H Folch, G Moran
Macrophages and neutrophils are important cellular components in the process of acute inflammation and its subsequent resolution, and evidence increasingly suggests that they play important functions during the resolution of chronic, adaptive inflammatory processes. Exacerbated neutrophil activity can be harmful to surrounding tissues; this is important in a range of diseases, including allergic asthma and chronic obstructive pulmonary disease in humans, and equine asthma (also known as recurrent airway obstruction (RAO)...
January 2, 2018: Veterinary Research Communications
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