Read by QxMD icon Read

Methylation rectal cancer

Ruoxu Dou, Reiko Nishihara, Yin Cao, Tsuyoshi Hamada, Kosuke Mima, Atsuhiro Masuda, Yohei Masugi, Yan Shi, Mancang Gu, Wanwan Li, Annacarolina da Silva, Katsuhiko Nosho, Xuehong Zhang, Jeffrey A Meyerhardt, Edward L Giovannucci, Andrew T Chan, Charles S Fuchs, Zhi Rong Qian, Shuji Ogino
Experimental evidence suggests that the let-7 family of noncoding RNAs suppresses adaptive immune responses, contributing to immune evasion by the tumor. We hypothesized that the amount of let-7a and let-7b expression in colorectal carcinoma might be associated with limited T-lymphocyte infiltrates in the tumor microenvironment and worse clinical outcome. Utilizing the molecular pathological epidemiology resources of 795 rectal and colon cancers in two U.S.-nationwide prospective cohort studies, we measured tumor-associated let-7a and let-7b expression levels by quantitative reverse-transcription PCR, and CD3(+), CD8(+), CD45RO (PTPRC)(+), and FOXP3(+) cell densities by tumor tissue microarray immunohistochemistry and computer-assisted image analysis...
October 13, 2016: Cancer Immunology Research
Jiufeng Wei, Guodong Li, Jinning Zhang, Yuhui Zhou, Shuwei Dang, Hongsheng Chen, Qiong Wu, Ming Liu
DNA methylation was regarded as the promising biomarker for rectal cancer diagnosis. However, the optimal methylation biomarkers with ideal diagnostic performance for rectal cancer are still limited. To identify new molecular markers for rectal cancer, we mapped DNA methylation and transcriptomic profiles in the six rectal cancer and paired normal samples. Further analysis revealed the hypermethylated probes in cancer prone to be located in gene promoter. Meanwhile, transcriptome analysis presented 773 low-expressed and 1,161 over-expressed genes in rectal cancer...
August 23, 2016: Oncotarget
Fang Zhao, Ekaterina Olkhov-Mitsel, Theodorus van der Kwast, Jenna Sykes, Darko Zdravic, Vasundara Venkateswaran, Alexandre R Zlotta, Andrew Loblaw, Neil E Fleshner, Laurence Klotz, Danny Vesprini, Bharati Bapat
PURPOSE: Prostate cancer (PCa) patients on active surveillance (AS) are monitored through repeated prostate-specific antigen (PSA) measurements, digital rectal exams (DREs) and prostate biopsies. A subset of AS patients will later "reclassify" with disease progression prompting definitive treatment. To minimize the risk of under-treating such AS patients, minimally-invasive tests incorporating biomarkers to identify patients who will reclassify are urgently needed. METHODS: We assessed post-DRE urine samples of AS patients for selected DNA methylation biomarkers that were previously investigated in radical prostatectomy specimens and shown to correlate with increasing risk of PCa...
August 18, 2016: Journal of Urology
Veronika Vymetalkova, Pavel Vodicka, Barbara Pardini, Fabio Rosa, Miroslav Levy, Michaela Schneiderova, Vaclav Liska, Ludmila Vodickova, Torbjörn K Nilsson, Sanja A Farkas
AIM: The aim of the present study is to address a genome-wide search for novel methylation biomarkers in the rectal cancer (RC), as only scarce information on methylation profile is available. MATERIALS & METHODS: We analyzed methylation status in 25 pairs of RC and adjacent healthy mucosa using the Illumina Human Methylation 450 BeadChip. RESULTS: We found significantly aberrant methylation in 33 genes. After validation of our results by pyrosequencing, we found a good agreement with our findings...
September 2016: Epigenomics
Kosuke Mima, Jonathan A Nowak, Zhi Rong Qian, Yin Cao, Mingyang Song, Yohei Masugi, Yan Shi, Annacarolina da Silva, Mancang Gu, Wanwan Li, Tsuyoshi Hamada, Xuehong Zhang, Kana Wu, Jeffrey A Meyerhardt, Hideo Baba, Edward L Giovannucci, Andrew T Chan, Charles S Fuchs, Shuji Ogino, Reiko Nishihara
Colorectal tumors arise with genomic and epigenomic alterations through interactions between neoplastic cells, immune cells, and microbiota that vary along the proximal to distal axis of colorectum. Long interspersed nucleotide element-1 (LINE-1) hypomethylation in colorectal cancer has been associated with worse clinical outcome. Utilizing 1,317 colon and rectal carcinoma cases in two U.S.-nationwide prospective cohort studies, we examined patient survival according to LINE-1 methylation level stratified by tumor location...
July 4, 2016: Oncotarget
Martha L Slattery, Jennifer S Herrick, Lila E Mullany, Erica Wolff, Michael D Hoffman, Daniel F Pellatt, John R Stevens, Roger K Wolff
MiRNAs regulate gene expression by post-transcriptionally suppressing mRNA translation or by causing mRNA degradation. It has been proposed that unique miRNAs influence specific tumor molecular phenotype. In this paper, we test the hypotheses that miRNA expression differs by tumor molecular phenotype and that those differences may influence prognosis. Data come from population-based studies of colorectal cancer conducted in Utah and the Northern California Kaiser Permanente Medical Care Program. A total of 1893 carcinoma samples were run on the Agilent Human miRNA Microarray V19...
August 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
Yohei Masugi, Reiko Nishihara, Juhong Yang, Kosuke Mima, Annacarolina da Silva, Yan Shi, Kentaro Inamura, Yin Cao, Mingyang Song, Jonathan A Nowak, Xiaoyun Liao, Katsuhiko Nosho, Andrew T Chan, Marios Giannakis, Adam J Bass, F Stephen Hodi, Gordon J Freeman, Scott Rodig, Charles S Fuchs, Zhi Rong Qian, Shuji Ogino
OBJECTIVE: Evidence suggests that CD274 (programmed death-ligand 1, B7-H1) immune checkpoint ligand repress antitumour immunity through its interaction with the PDCD1 (programmed cell death 1, PD-1) receptor of T lymphocytes in various tumours. We hypothesised that tumour CD274 expression levels might be inversely associated with T-cell densities in colorectal carcinoma tissue. DESIGN: We evaluated tumour CD274 expression by immunohistochemistry in 823 rectal and colon cancer cases within the Nurses' Health Study and Health Professionals Follow-up Study...
May 5, 2016: Gut
Jeong Mo Bae, Jung Ho Kim, Gyeong Hoon Kang
CONTEXT: -Colorectal cancer is a heterogeneous disease entity with 3 molecular carcinogenesis pathways and 2 morphologic multistep pathways. Right-sided colon cancers and left-sided colon and rectal cancers exhibit differences in their incidence rates according to geographic region, age, and sex. A linear tendency toward increasing frequencies of microsatellite instability-high or CpG island methylator phenotype-high cancers in subsites along the bowel from the rectum to the cecum or the ascending colon accounts for the differences in tumor phenotypes associated with these subsites...
May 2016: Archives of Pathology & Laboratory Medicine
Xiaoliang Wang, Junwei Fan, Fudong Yu, Feifei Cui, Xing Sun, Lin Zhong, Dongwang Yan, Chongzhi Zhou, Guilong Deng, Bin Wang, Xiaosheng Qi, Shuyun Wang, Lei Qu, Biao Deng, Ming Pan, Jian Chen, Yupeng Wang, Guohe Song, Huamei Tang, Zongguang Zhou, Zhihai Peng
The aim of the present study was to determine whether MALL expression is associated with colon cancer progression and patient survival. MALL mRNA expression was reduced in the tumor tissues of 70% of the colon cancer patients and 75% of the rectal cancer patients as compared to their normal tissues. MALL protein was also significantly reduced in the tumor tissues of colon cancer patients (P < 0.001). Increased LOH and methylation of MALL was observed in tumor tissues as compared to normal tissues. Reduced MALL expression was associated with vessin invasion, disease recurrence and metastasis or death (P ≤ 0...
April 19, 2016: Oncotarget
Kosuke Mima, Reiko Nishihara, Juhong Yang, Ruoxu Dou, Yohei Masugi, Yan Shi, Annacarolina da Silva, Yin Cao, Mingyang Song, Jonathan Nowak, Mancang Gu, Wanwan Li, Teppei Morikawa, Xuehong Zhang, Kana Wu, Hideo Baba, Edward L Giovannucci, Jeffrey A Meyerhardt, Andrew T Chan, Charles S Fuchs, Zhi Rong Qian, Shuji Ogino
PURPOSE: Prostaglandin-endoperoxide synthase 2 (PTGS2, cyclooxygenase-2; a target of aspirin) produces inflammatory mediator prostaglandin E2 (PGE2), and contributes to colorectal neoplasia development. PTGS2-driven inflammatory responses can induce tumor expression of microRNA MIR21 (miR-21) that can increase local PGE2 level by downregulating PGE2-metabolizing enzymes. We hypothesized that the prognostic association of tumor MIR21 expression level in colorectal carcinoma might depend on inflammatory tumor microenvironment and be stronger in tumors expressing high-level PTGS2...
August 1, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Masakazu Miyake, Takashi Takeda, Naotsugu Haraguchi, Mamoru Uemura, Masataka Ikeda, Michihiko Miyazaki, Sakae Maeda, Kazuyoshi Yamamoto, Naoki Hama, Kazuhiro Nishikawa, Atsushi Miyamoto, Motohiro Hirao, Shoji Nakamori, Mitsugu Sekimoto
We report of a 74-year-old man with advanced rectal cancer invading the small intestine and bladder. Initially, 3 courses of neoadjuvant chemotherapy with FOLFIRI and cetuximab were administered. At the beginning of the 4 course, the patient experienced fever and dyspnea. Chest radiography and computed tomography showed ground-grass opacities in both lungs. He was diagnosed with interstitial pneumonia induced by irinotecan or cetuximab. Steroid pulse therapy consisting of methyl-prednisolone (1,000 mg/day; 3 days) with respiratory assist resulted in significant improvement, and prednisolone was continued while reducing the dose gradually...
November 2015: Gan to Kagaku Ryoho. Cancer & Chemotherapy
J Li, K L Huang, T Zhang, H Li, J Zhao, H Wang
Adenocarcinomas are tumors of glandular characteristics. While tissues of common origins have been known to undergo similar epigenetic changes, it is unclear whether adenocarcinomas of different cancer types would exhibit similar DNA methylation and epigenetic regulation profiles. Herein, we studied global methylation and mRNA expression levels in 1214 lung, prostate, colon, and rectal cancer samples from The Cancer Genome Atlas (TCGA). We identified 602 candidate epigenetically silenced genes shared across these cancer types, and 835 associated CpG sites...
2016: Neoplasma
A Joan Levine, Amanda I Phipps, John A Baron, Daniel D Buchanan, Dennis J Ahnen, Stacey A Cohen, Noralane M Lindor, Polly A Newcomb, Christophe Rosty, Robert W Haile, Peter W Laird, Daniel J Weisenberger
BACKGROUND: The CpG island methylator phenotype (CIMP) is a major molecular pathway in colorectal cancer. Approximately 25% to 60% of CIMP tumors are microsatellite unstable (MSI-H) due to DNA hypermethylation of the MLH1 gene promoter. Our aim was to determine if the distributions of clinicopathologic factors in CIMP-positive tumors with MLH1 DNA methylation differed from those in CIMP-positive tumors without DNA methylation of MLH1. METHODS: We assessed the associations between age, sex, tumor-site, MSI status BRAF and KRAS mutations, and family colorectal cancer history with MLH1 methylation status in a large population-based sample of CIMP-positive colorectal cancers defined by a 5-marker panel using unconditional logistic regression to assess the odds of MLH1 methylation by study variables...
January 2016: Cancer Epidemiology, Biomarkers & Prevention
Paul Lochhead, Reiko Nishihara, Zhi Rong Qian, Kosuke Mima, Yin Cao, Yasutaka Sukawa, Sun A Kim, Kentaro Inamura, Xuehong Zhang, Kana Wu, Edward Giovannucci, Jeffrey A Meyerhardt, Andrew T Chan, Charles S Fuchs, Shuji Ogino
BACKGROUND: Observational data have suggested that intakes of nutrients involved in one-carbon metabolism are inversely associated with risk of colorectal carcinoma and adenomas. In contrast, results from some preclinical studies and cardiovascular and chemoprevention trials have raised concerns that high folate intake may promote carcinogenesis by facilitating the progression of established neoplasia. OBJECTIVE: We tested the hypothesis that higher total folate intake (including food folate and folic acid from fortified foods and supplements) or other one-carbon nutrient intakes might be associated with poorer survival after a diagnosis of colorectal cancer...
November 2015: American Journal of Clinical Nutrition
Ruth Exner, Walter Pulverer, Martina Diem, Lisa Spaller, Laura Woltering, Martin Schreiber, Brigitte Wolf, Markus Sonntagbauer, Fabian Schröder, Judith Stift, Fritz Wrba, Michael Bergmann, Andreas Weinhäusel, Gerda Egger
BACKGROUND: Aberrant DNA methylation is more prominent in proximal compared with distal colorectal cancers. Although a number of methylation markers were identified for colon cancer, yet few are available for rectal cancer. METHODS: DNA methylation differences were assessed by a targeted DNA microarray for 360 marker candidates between 22 fresh frozen rectal tumour samples and 8 controls and validated by microfluidic high-throughput and methylation-sensitive qPCR in fresh frozen and formalin-fixed paraffin-embedded (FFPE) samples, respectively...
September 29, 2015: British Journal of Cancer
José Perea, Juana M Cano, Daniel Rueda, Juan L García, Lucía Inglada, Irene Osorio, María Arriba, Jessica Pérez, Miriam Gaspar, Tamara Fernández-Miguel, Yolanda Rodríguez, Javier Benítez, Rogelio González-Sarmiento, Miguel Urioste
Early-onset Colorectal Cancer (ECRC) represents a significant and increasing proportion of Colorectal Cancer (CRC), but it is a heterogeneous entity that probably encompasses specific subclasses. On the premise that the carcinogenetic mechanism and progression of CRC may differ with location, we analyzed molecular and clinical characteristics of ECRC according to tumor location in order to identify more homogeneous subgroups of CRC. Right-sided ECRC is a subset in which most Lynch Syndrome cases are found, with earlier stages at diagnosis and better prognosis...
2015: American Journal of Cancer Research
Kosuke Mima, Reiko Nishihara, Zhi Rong Qian, Yin Cao, Yasutaka Sukawa, Jonathan A Nowak, Juhong Yang, Ruoxu Dou, Yohei Masugi, Mingyang Song, Aleksandar D Kostic, Marios Giannakis, Susan Bullman, Danny A Milner, Hideo Baba, Edward L Giovannucci, Levi A Garraway, Gordon J Freeman, Glenn Dranoff, Wendy S Garrett, Curtis Huttenhower, Matthew Meyerson, Jeffrey A Meyerhardt, Andrew T Chan, Charles S Fuchs, Shuji Ogino
OBJECTIVE: Accumulating evidence links the intestinal microbiota and colorectal carcinogenesis. Fusobacterium nucleatum may promote colorectal tumour growth and inhibit T cell-mediated immune responses against colorectal tumours. Thus, we hypothesised that the amount of F. nucleatum in colorectal carcinoma might be associated with worse clinical outcome. DESIGN: We used molecular pathological epidemiology database of 1069 rectal and colon cancer cases in the Nurses' Health Study and the Health Professionals Follow-up Study, and measured F...
August 26, 2015: Gut
Andrea Imperatori, Nicola Rotolo, Lorenzo Dominioni, Elisa Nardecchia, Maria Cattoni, Laura Cimetti, Cristina Riva, Fausto Sessa, Daniela Furlan
BACKGROUND: Treatment of pulmonary recurrence from colorectal cancer involving the main bronchus usually entails palliation using interventional bronchoscopy, because the prognosis is generally very poor. Surgical experience has clarified that in this setting pneumonectomy should only be performed in carefully selected patients showing favorable prognostic profiles (defined by low carcinoembryonic antigen serum levels pre-thoracotomy), solitary and completely resectable pulmonary metastasis, and long disease-free intervals...
2015: BMC Cancer
Jeremy S Williamson, Dean A Harris, John Beynon, Gareth J S Jenkins
There is much debate around the preoperative treatment of colorectal cancer and, in particular, neoadjuvant chemoradiotherapy in locally advanced rectal cancer. This treatment carries a significant risk of harmful side effects and has a highly variable response rate. Predictive biomarkers have been the subject of a great deal of study with the aim of pretreatment risk stratification in order to more accurately determine which patients will derive the most benefit and least harm from these treatments. The study of epigenetics in colorectal cancer is relatively recent, and distinct patterns of aberrant DNA methylation, in particular the cytosine-phosphate-guanine (CpG) island methylator phenotype (CIMP), have been demonstrated in colorectal cancer, and their characterisation and significance are under debate, particularly in rectal cancer...
2015: Clinical Epigenetics
Kosuke Mima, Yasutaka Sukawa, Reiko Nishihara, Zhi Rong Qian, Mai Yamauchi, Kentaro Inamura, Sun A Kim, Atsuhiro Masuda, Jonathan A Nowak, Katsuhiko Nosho, Aleksandar D Kostic, Marios Giannakis, Hideo Watanabe, Susan Bullman, Danny A Milner, Curtis C Harris, Edward Giovannucci, Levi A Garraway, Gordon J Freeman, Glenn Dranoff, Andrew T Chan, Wendy S Garrett, Curtis Huttenhower, Charles S Fuchs, Shuji Ogino
IMPORTANCE: Evidence indicates a complex link between gut microbiome, immunity, and intestinal tumorigenesis. To target the microbiota and immunity for colorectal cancer prevention and therapy, a better understanding of the relationship between microorganisms and immune cells in the tumor microenvironment is needed. Experimental evidence suggests that Fusobacterium nucleatum may promote colonic neoplasia development by downregulating antitumor T cell-mediated adaptive immunity. OBJECTIVE: To test the hypothesis that a greater amount of F nucleatum in colorectal carcinoma tissue is associated with a lower density of T cells in tumor tissue...
August 2015: JAMA Oncology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"