keyword
https://read.qxmd.com/read/38213357/a-rare-case-of-polymerase-chain-reaction-negative-severe-clostridioides-difficile-infection
#1
Charity C Iheagwara, Carlos Cantu Lopez, Onyinye N Otaluka, Byron Okwesili, Vadim Belinski, Ala Muhanna, Bereket Tewoldemedhin, Jihad Slim, Maria Szabela, Jack Boghossian, Yatinder Bains
Accurately diagnosing Clostridioides difficile infection (CDI) is crucial for effective patient management. A misdiagnosis poses risks to patients, leads to treatment delays, and contributes to infection transmission in healthcare settings. While using polymerase chain reaction (PCR) to amplify the toxin B gene is a sensitive method for detecting toxigenic C. difficile , there is still a risk of false-negative results. These inaccuracies could have significant consequences for diagnosing and treating CDI, emphasizing the need for careful consideration and other diagnostic approaches...
December 2023: Curēus
https://read.qxmd.com/read/37842346/factors-associated-with-fecal-microbiota-transplant-failure-in-the-treatment-of-recurrent-clostridioides-difficile-infection-a-single-center-retrospective-study
#2
JOURNAL ARTICLE
Fatima Warraich, Syed H Sohail, Alexander Knee, Jacob Smith, Hans Schlecht, Daniel Skiest
Background Clostridioides difficile infection (CDI) is a major cause of hospital-acquired diarrhea and is associated with substantial morbidity and mortality. Recurrences following treatment are common. Fecal microbiota transplantation (FMT) is a therapeutic intervention in which stool from a healthy donor is administered to a patient with recurrent CDI. Studies to date of predictors of FMT failure have primarily included inpatients. In this study, we aimed to describe FMT failure rates within one year of FMT and evaluate factors associated with FMT failure...
September 2023: Curēus
https://read.qxmd.com/read/36814219/role-of-foetal-kidney-size-on-kidney-function-in-childhood-the-born-in-bradford-cohort-renal-study
#3
JOURNAL ARTICLE
Nida Ziauddeen, Robin F Jeffrey, Dagmar Waiblinger, Simon Ds Fraser, Nisreen A Alwan, Ho M Yuen, Rafaq Azad, Dan Mason, John Wright, Richard Jm Coward, Paul J Roderick
BACKGROUND: Foetal and early childhood development contributes to the risk of adult non-communicable diseases such as hypertension and cardiovascular disease. We aimed to investigate whether kidney size at birth is associated with markers of kidney function at 7-11 years. METHODS: Foetal kidney dimensions were measured using ultrasound scans at 34 weeks gestation and used to derive kidney volume (cm3 ) in 1802 participants in the Born in Bradford (BiB) birth cohort...
February 22, 2023: BMC Nephrology
https://read.qxmd.com/read/36609791/case-of-mastocytemia-and-systemic-mastocytosis-in-a-dog-procyte-dx-and-sysmex-xt-2000i-scattergram-findings-morphologic-features-and-c-kit-somatic-mutation-analysis
#4
Jelena Palić, Annabelle Heier, Els Acke, Franziska Hergt, Maria Elena Turba
A 10-year-old female Golden Retriever was presented for a recheck after the complete removal of low-grade complex mammary carcinoma. The in-house ProCyte Dx automated counts revealed moderate regenerative anemia and moderate eosinophilia. The ProCyte Dx WBC scattergram showed a cloud in an unusual place parallel and to the right of the monocyte dot plot location. Cells were classified as either monocytes or neutrophils with no clear separation. Complete blood count analysis performed in the laboratory on a Sysmex XT-2000iV analyzer showed moderate regenerative anemia and WBC count within RI; a differential count was not provided by the instrument...
June 2023: Veterinary Clinical Pathology
https://read.qxmd.com/read/36219324/prevalence-and-seasonality-of-clostridiodes-difficile-over-12-years-at-a-tertiary-hospital-in-brazil
#5
JOURNAL ARTICLE
Adriane C Maestri, Paula K Ando, Gustavo Sarot, Fernanda de Castilho, Sonia M Raboni, Keite S Nogueira
Clostridioides difficile is the main pathogen responsible for antibiotic-associated diarrhea in adults. Besides its challenging diagnosis, C. difficile infection (CDI) causes substantial morbidity and mortality. Commercially, there are assays with different targets and performances in sensitivity and specificity. The objectives of this study were to: (1) evaluate the prevalence and seasonal variability of CDI rates at a tertiary hospital in southern Brazil over 12 years and (2) determine the impact of using a two-step algorithm test in the laboratory diagnosis...
October 11, 2022: Current Microbiology
https://read.qxmd.com/read/35967141/advanced-case-of-cardiac-amyloidosis-presents-with-chronic-diarrhea
#6
Mahmoud Abdelsamia, Osama Mosalem, Yasser Radwan, Manal Boumegouas, Heather Laird Fick
Late diagnosis of light chain (AL) amyloidosis can lead to catastrophic consequences on the quality of life of affected patients and overall disease prognosis. Therefore, clinicians should have high suspicion and recognize clinical red flags for amyloidosis. This case report presents a 65-year-old female who presented to the emergency department with chronic diarrhea and significant weight loss with significant hypotension. The patient was treated four weeks prior to admission with a five-day course of nitrofurantoin for urinary tract infection...
July 2022: Curēus
https://read.qxmd.com/read/35509400/hematological-findings-in-adult-patients-with-sars-cov-2-infection-at-tygerberg-hospital-cape-town-south-africa
#7
JOURNAL ARTICLE
Ibtisam Abdullah, Helena M Cornelissen, Ernest Musekwa, Annalise Zemlin, Thumeka Jalavu, Nomusa Mashigo, Carissa Chetty, Nokwazi Nkosi, Zivanai C Chapanduka
Background: Coronavirus disease 2019 (COVID-19) is associated with hematological abnormalities of variable severity. The full blood count (FBC) and leukocyte differential count (DIFF) could facilitate the prediction of disease severity and outcome in COVID-19. This study aimed to assess the hematological parameters in early severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and their correlation with disease outcome. Methods: A retrospective cross-sectional descriptive study was performed...
May 2022: Health Science Reports
https://read.qxmd.com/read/35078721/rare-toxin-a-negative-and-toxin-b-positive-strain-of-clostridioides-difficile-from-japan-lacking-a-complete-tcda-gene
#8
JOURNAL ARTICLE
Yukiko Takemori-Sakai, Kenji Satou, Yasuko Senda, Youko Nakamura, Hatsumi Otani, Akiko Maekawa, Hiroyasu Oe, Megumi Oshima, Shiori Yoneda-Nakagawa, Taro Miyagawa, Koichi Sato, Hisayuki Ogura, Mika Mori, Taizo Wada, Yoshio Sakai, Masahiro Yutani, Takuhiro Matsumura, Yukako Fujinaga, Toshifumi Gabata, Takashi Wada, Yasunori Iwata
INTRODUCTION: Clostridioides difficile (C. difficile) produces three kinds of toxins: toxin A (enterotoxin), toxin B (cytotoxin), and C. difficile transferase (CDT), a binary toxin. Some strains show positivity only for toxin B. These strains reportedly possess a gene for toxin A, tcdA. However, toxin A production is inhibited due to a mutated stop codon and/or deletion within the tcdA gene. Here for the first case in Japan, we describe toxin genomes and proteins of a strain possessing only toxin B and lacking a complete tcdA gene, along with clinical manifestations...
May 2022: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://read.qxmd.com/read/34338715/prevalence-of-detection-of-clostridioides-difficile-among-asymptomatic-children-a-systematic-review-and-meta-analysis
#9
JOURNAL ARTICLE
Sarah R Tougas, Nidhi Lodha, Ben Vandermeer, Diane L Lorenzetti, Phillip I Tarr, Gillian A M Tarr, Linda Chui, Otto G Vanderkooi, Stephen B Freedman
IMPORTANCE: Detection of Clostridioides difficile has frequently been described in asymptomatic infants and children, but accurate estimates across the age spectrum are unavailable. OBJECTIVE: To assess the prevalence of C difficile detection among asymptomatic children across the age spectrum. DATA SOURCES: This systematic review and meta-analysis included a search of the Cochrane Central Register of Controlled Trials, MEDLINE, Embase, CINAHL, Scopus, and Web of Science for articles published from January 1, 1990, to December 31, 2020...
October 1, 2021: JAMA Pediatrics
https://read.qxmd.com/read/34184680/rapid-ultra-sensitive-diagnosis-of-clostridium-difficile-infection-using-a-sers-based-lateral-flow-assay
#10
JOURNAL ARTICLE
Waleed A Hassanain, Julia Spoors, Christopher L Johnson, Karen Faulds, Neil Keegan, Duncan Graham
Clostridium difficile (C. diff) infection is one of the most contagious diseases associated with high morbidity and mortality rates in hospitalised patients. Accurate diagnosis can slow its spread by determining the most effective treatment. Herein, we report a novel testing platform as a proof-of-concept for the selective, sensitive, rapid and cost-effective diagnosis of C. diff infection (CDI) based on a duplex measurement. This was achieved by detecting two specific biomarkers, surface layer protein A (SlpA) and toxin B (ToxB), using a surface enhanced Raman scattering-based lateral flow assay (SERS-based LFA)...
June 29, 2021: Analyst
https://read.qxmd.com/read/33601876/significance-of-a-polymerase-chain-reaction-method-in-the-detection-of-clostridioides-difficile
#11
JOURNAL ARTICLE
J Jaqueti Aroca, L M Molina Esteban, I García-Arata, J García-Martínez, I Cano De Torres, S Prieto Menchero
OBJECTIVE: Clostridioides difficile (CD) is the most common cause of nosocomial diarrhea. Detection of CD toxin in patients' faecal samples is the traditional rapid method for the diagnosis of CD infection. Various testing algorithms have been proposed: an initial screening test using a rapid test, and a confirmatory test (cytotoxicity neutralization assay, toxigenic culture, nucleic acid amplification test) for discordant results. The aim of this study was to evaluate the effectiveness of a two-step algorithm using an immunochromatographic test followed of a polymerase chain reaction (PCR)...
April 2021: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://read.qxmd.com/read/32249275/bifidobacterium-infantis-relieves-allergic-asthma-in-mice-by-regulating-th1-th2
#12
JOURNAL ARTICLE
Wenlan Wang, Xiaoming Luo, Qin Zhang, Xujun He, Zhifang Zhang, Xinxin Wang
BACKGROUND Bifidobacteria are among the probiotics used in treating intestinal diseases and are rarely used for allergic asthma treatment. The present study investigated the mechanism of B. infantis in treating allergic asthma in mice. MATERIAL AND METHODS A total of 40 male Balb/c mice were randomized into control, ovalbumin (OVA), montelukast (Mon), and B. infantis (B10) groups, and allergic asthma was induced in the OVA, Mon, and B10 groups. Airway reactivity was measured on day 29 by methacholine at various doses...
April 6, 2020: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://read.qxmd.com/read/32119956/detection-of-c-difficile-toxin-as-a-model-assay-for-performing-fully-automated-high-throughput-rt-pcr-on-clinical-stool-samples
#13
JOURNAL ARTICLE
Ulrich Eigner, Dominik Nörz, Svenja Reucher, Jan Furrer, Jingtao Sun, Kristina Chu, Melissa Kolb, Nadine Hefner, Susanne Pfefferle, Marc Lütgehetmann
BACKGROUND: The cobas® omni Utility Channel enables users to integrate lab-developed tests (LDTs) on the cobas® 6800 System to perform molecular diagnostics with high-throughput capacity and full automation. At present, there are no CE- or FDA-approved tests for stool pathogens on this system. To assess the performance of stool as a matrix, we evaluated the analytical and clinical performance of an LDT for detection of Clostridioides difficile (C. difficile) toxin B using the Utility Channel (C...
May 2020: Journal of Microbiological Methods
https://read.qxmd.com/read/32051264/evaluation-of-cycle-threshold-toxin-concentration-and-clinical-characteristics-of-clostridioides-difficile-infection-in-patients-with-discordant-diagnostic-test-results
#14
JOURNAL ARTICLE
Megan D Shah, Joan-Miquel Balada-Llasat, Kelci Coe, Erica Reed, Johanna Sandlund, Preeti Pancholi
Background: Clostridioides difficile infection (CDI) is one of the most common health-care associated infections that can cause significant morbidity and mortality. CDI diagnosis involves laboratory testing in conjunction with clinical assessment. The objective of this study was to assess the performance of various C. difficile tests and to compare clinical characteristics, Xpert® C. difficile /Epi (PCR) cycle threshold (CT), and Singulex Clarity® C. diff toxins A/B (Clarity) concentrations between groups with discordant test results...
February 12, 2020: Journal of Clinical Microbiology
https://read.qxmd.com/read/31941691/prospective-evaluation-of-the-maripoc-test-for-detection-of-clostridioides-difficile-glutamate-dehydrogenase-and-toxins-a-b
#15
JOURNAL ARTICLE
Roosa Savolainen, Juha M Koskinen, Silja Mentula, Janne O Koskinen, Suvi-Sirkku Kaukoranta
The objective of this study was to evaluate a novel automated random-access test, mariPOC CDI (ArcDia Ltd., Finland), for the detection of Clostridioides difficile glutamate dehydrogenase (GDH) and toxins A and B directly from fecal specimens. The mariPOC test was compared with both the GenomEra C. difficile PCR assay (Abacus Diagnostica Oy, Finland) and the TechLab C. diff Quik Chek Complete (Alere Inc.; now Abbot) membrane enzyme immunoassay (MEIA). Culture and the Xpert C. difficile assay (Cepheid Inc., USA) were used to resolve discrepant results...
March 25, 2020: Journal of Clinical Microbiology
https://read.qxmd.com/read/31776192/high-agreement-between-an-ultrasensitive-clostridioides-difficile-toxin-assay-and-a-c-difficile-laboratory-algorithm-utilizing-gdh-and-toxin-enzyme-immunoassays-and-cytotoxin-testing
#16
JOURNAL ARTICLE
Marie L Landry, Jeffrey E Topal, Joel Estis, Phoebe Katzenbach, Niamh Nolan, Johanna Sandlund
The Singulex Clarity C. diff toxins A/B (Clarity) assay is an automated, ultrasensitive immunoassay for the detection of Clostridioides difficile toxins in stool. In this study, the performance of the Clarity assay was compared to that of a multistep algorithm using an enzyme immunoassay (EIA) for detection of glutamate dehydrogenase (GDH) and toxins A and B arbitrated by a semiquantitative cell cytotoxicity neutralization assay (CCNA). The performance of the assay was evaluated using 211 residual deidentified stool samples tested with a GDH-and-toxin EIA (C...
January 28, 2020: Journal of Clinical Microbiology
https://read.qxmd.com/read/31266088/-first-experiences-with-detection-of-the-tcdb-gene-of-clostridioides-difficile-using-simplexa-c-difficile-direct-kit
#17
JOURNAL ARTICLE
Martin Kracík
OBJECTIVE: Clostridioides difficile (formerly Clostridium difficile) is one of the main pathogens causing nosocomial infections today. It colonizes the intestines of patients receiving antibiotic therapy, causing unpleasant or even life-threatening conditions (diarrhea, toxic megacolon). Rapid and correct detection of strain toxigenicity is essential for treatment and isolation of patients. Simplexa C. difficile Direct Kit is a real-time PCR kit detecting the tcdB gene of C. difficile...
March 2019: Klinická Mikrobiologie a Infekc̆ní Lékar̆ství
https://read.qxmd.com/read/31129008/laboratory-comparison-between-cell-cytotoxicity-neutralization-assay-and-ultrasensitive-single-molecule-counting-technology-for-detection-of-clostridioides-difficile-toxins-a-and-b-pcr-enzyme-immunoassays-and-multistep-algorithms
#18
COMPARATIVE STUDY
Johanna Sandlund, Ray Mills, Christen Griego-Fullbright, Aaron Wagner, Joel Estis, Amelita Bartolome, Anna Almazan, Stanley Tam, Sheryl Biscocho, Salina Abusali, Niamh Nolan, Jeffrey J Bishop, John Todd, Stephen Young
Diagnostic tests for Clostridioides difficile infection (CDI) lack either specificity (nucleic acid amplification tests) or sensitivity (enzyme immunoassays; EIAs). The performance of the Singulex Clarity® C. diff toxins A/B assay was compared to cell cytotoxicity neutralization assay. Testing was also performed using an EIA for glutamate dehydrogenase (GDH) and C. difficile toxins A and B (C. Diff Quik Chek Complete®), polymerase chain reaction (PCR) (BD MAX™ Cdiff Assay), and 2 multistep algorithms: algorithm 1 (discordant GDH/toxin results arbitrated by PCR) and algorithm 2 (PCR-positive samples tested with toxin EIA)...
September 2019: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/31101982/interference-of-mir-943-3p-with-secreted-frizzled-related-proteins4-sfrp4-in-an-asthma-mouse-model
#19
JOURNAL ARTICLE
Jian Shen, Jun Zhao, Qing-Yan Ye, Xi-Dong Gu
The aim of this study is to investigate the potential roles of miR-943-3p and its target gene secreted frizzled-related proteins4 (SFRP4) in allergic asthma and elucidate its underlying mechanism, which may prompt a new clue about developing novel treatments of this disease. An allergic asthma mouse model was generated by challenging with ovalbumin (OVA); lung pathological features of mice were viewed using H&E staining; thickness of subepithelial fibrosis and smooth muscle was measured using Masson's trichrome staining...
October 2019: Cell and Tissue Research
https://read.qxmd.com/read/30802215/anti-asthmatic-effects-of-volatile-organic-compounds-from-chamaecyparis-obtusa-pinus-densiflora-pinus-koraiensis-or-larix-kaempferi-wood-panels
#20
COMPARATIVE STUDY
C Ahn, Y-J Jang, J-W Kim, M-J Park, Y-M Yoo, E-B Jeung
Asthma is a common chronic inflammatory disease in which lung airways narrow and produce extra mucus. Numerous medications, such as steroids, are used to prevent or control asthmatic symptoms, but side effects are associated with those medications. There are reports of anti-inflammatory, antibacterial, and antiparasitic effects of terpene, a volatile organic compound (VOC) in conifers. VOCs easily enter a gaseous form, and wood products are good sources of VOCs. However, only a few studies have been conducted on the effect on asthma of VOCs emitted by wood...
December 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
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