keyword
MENU ▼
Read by QxMD icon Read
search

C diff and PCR

keyword
https://www.readbyqxmd.com/read/29103272/first-case-of-canine-infection-with-hepatozoon-canis-apicomplexa-haemogregarinidae-in-the-republic-of-korea
#1
Seung-Joo Kwon, Yoon-Hee Kim, Hyun-Hee Oh, Ul-Soo Choi
This report describes a dog infected with Hepatozoon canis, the first canine infection in the Republic of Korea. A 2-year-old intact male Maltese dog presented with anorexia and depression. Physical examinations revealed mild dehydration and hyperthermia (39.8°C), and blood analysis showed pancytopenia. Diff-Quik staining of blood smear specimens showed the presence of ellipsoidal shaped structures (gamonts of H. canis) within a small number of neutrophils. Real-time PCR analysis using whole blood confirmed infection by H...
October 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28751156/performance-evaluation-of-the-verigene-%C3%A2-clostridium-difficile-nucleic-acid-test-an-automated-multiplex-molecular-testing-system-for-detection-of-c-%C3%A2-difficile-toxin
#2
Kosuke Kosai, Yuki Iwanaga, Norihiko Akamatsu, Yuya Okada, Norihito Kaku, Naoki Uno, Yoshitomo Morinaga, Hiroo Hasegawa, Taiga Miyazaki, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara
The Verigene(®)Clostridium difficile nucleic acid test (Verigene(®) CDF test) is an automatic and rapid detection system for the genes encoding tcdA, tcdB, binary toxin, and the single nucleotide deletion at base pair 117 in the tcdC based on microarray and PCR amplification. We compared the performance of the Verigene(®) CDF test to that of two enzyme immunoassays, C. DIFF QUIK CHEK COMPLETE and X/Pect Toxin A/B, using 118 specimens. We found overall concordance rates of 81.4% and 78.8% between C. DIFF QUIK CHEK COMPLETE and Verigene(®) CDF test, and X/Pect Toxin A/B and Verigene(®) CDF test...
October 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28615471/clostridium-difficile-pcr-cycle-threshold-predicts-free-toxin
#3
Fiona Senchyna, Rajiv L Gaur, Saurabh Gombar, Cynthia Y Truong, Lee F Schroeder, Niaz Banaei
There is no stand-alone Clostridium difficile diagnostic that can sensitively and rapidly detect fecal free toxins. We investigated the performance of the C. difficile PCR cycle threshold (CT ) for predicting free toxin status. Consecutive stool samples (n = 312) positive for toxigenic C. difficile by the GeneXpert C. difficile/Epi tcdB PCR assay were tested with the rapid membrane C. Diff Quik Chek Complete immunoassay (RMEIA). RMEIA toxin-negative samples were tested with the cell cytotoxicity neutralization assay (CCNA) and tgcBIOMICS enzyme-linked immunosorbent assay (ELISA)...
September 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28215602/clostridium-difficile-infection-in-acute-flares-of-inflammatory-bowel-disease-a-prospective-study
#4
Harry Sokol, Valérie Lalande, Cecilia Landman, Anne Bourrier, Isabelle Nion-Larmurier, Sylvie Rajca, Julien Kirchgesner, Philippe Seksik, Jacques Cosnes, Frédéric Barbut, Laurent Beaugerie
OBJECTIVES: Clostridium difficile infection (CDI) is a common complication in inflammatory bowel disease (IBD) and has been associated with poor IBD outcome. The aims of our study were to look for predictive factors of CDI in patients hospitalized for IBD flare and to evaluate a rapid testing strategy in this population. METHODS: Consecutive patients hospitalized for IBD flare in Saint-Antoine Hospital (Paris, France) were prospectively tested for CDI with a defined strategy involving rapid testing and reference methods...
June 2017: Digestive and Liver Disease
https://www.readbyqxmd.com/read/28181032/comprehensive-evaluation-of-chemiluminescent-immunoassays-for-the-laboratory-diagnosis-of-clostridium-difficile-infection
#5
A Makristathis, I Zeller, D Mitteregger, M Kundi, A M Hirschl
For the microbiological diagnosis of a Clostridium (C.) difficile infection (CDI), a two-test algorithm consisting of a C. difficile glutamate dehydrogenase (GDH)-immunoassay followed by a toxin-immunoassay in positive cases is widely used. In this study, two chemiluminescent immunoassays (CLIAs), one for GDH and the other for the toxins A and B, have been evaluated systematically using appropriate reference methods. Three-hundred diarrhoeal stool specimens submitted for CDI diagnosis were analysed by the LIAISON CLIAs (DiaSorin)...
July 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27651167/clostridium-difficile-infection-diagnostics-evaluation-of-the-c-diff-quik-chek-complete-assay-a-rapid-enzyme-immunoassay-for-detection-of-toxigenic-c-difficile-in-clinical-stool-samples
#6
COMPARATIVE STUDY
Karin Johansson, Hanna Karlsson, Torbjörn Norén
Diagnostic testing for Clostridium difficile infection (CDI) has, in recent years, seen the introduction of rapid dual-EIA (enzyme immunoassay) tests combining species-specific glutamate dehydrogenase (GDH) with toxin A/B. In a prospective study, we compared the C. DIFF Quik Chek Complete test to a combination of selective culture (SC) and loop-mediated isothermal amplification (LAMP) of the toxin A gene. Of 419 specimens, 68 were positive in SC including 62 positive in LAMP (14.7%). The combined EIA yielded 82 GDH positives of which 47 were confirmed toxin A/B positive (11%) corresponding to a sensitivity and specificity of 94% for GDH EIA compared to SC and for toxin A/B EIA a sensitivity of 71% and a specificity of 99% compared to LAMP...
November 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27625418/evaluation-of-the-performance-of-c-diff-quik-chek-complete-and-its-usefulness-in-a-hospital-setting-with-a-high-prevalence-of-clostridium-difficile-infection
#7
Hae-Sun Chung, Miae Lee
Rapid and accurate diagnosis of Clostridium difficile infection (CDI) is crucial for patient care, infection control, and efficient surveillance. We evaluated C. DIFF QUIK CHEK COMPLETE (QCC; TechLab), which detects glutamate dehydrogenase (GDH) antigen (QCC-Ag) and toxin A/B (QCC-Tox) simultaneously, and compared it to the laboratory diagnostics for CDI currently in use in a tertiary hospital setting with a high prevalence of CDI. QCC, RIDASCREEN C. difficile toxin A/B assay (Toxin EIA; R-Biopharm AG), chromID C...
January 2017: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/27552785/multiplex-pcr-assay-for-identifi-cation-and-differentiation-of-campylobacter-jejuni-and-campylobacter-coli-isolates
#8
Maria R Pavlova, Elina G Dobreva, Katucha I Ivanova, Galina D Asseva, Ivan N Ivanov, Peter K Petrov, Valeri R Velev, Ivelina I Tomova, Maida M Tiholova, Todor V Kantardjiev
INTRODUCTION: Campylobacter spp. are important causative agents of gastrointestinal infections in humans. The most frequently isolated strains of this bacterial genus are Campylobacter jejuni and Campylobacter coli. To date, genetic methods for bacterial identification have not been used in Bulgaria. We optimized the multiplex PSR assay to identify Campylobacter spp. and differentiate C. jejuni from C. coli in clinical isolates. We also compared this method with the routinely used biochemical methods...
April 2016: Folia Medica
https://www.readbyqxmd.com/read/27465145/characterisation-of-clostridium-difficile-strains-isolated-from-groote-schuur-hospital-cape-town-south-africa
#9
B Kullin, T Brock, N Rajabally, F Anwar, G Vedantam, S Reid, V Abratt
The C. difficile infection rate in South Africa is concerning. Many strains previously isolated from diarrhetic patients at Groote Schuur Hospital were ribotype 017. This study further characterised these strains with respect to their clonal relationships, antibiotic susceptibility, toxin production and various attributes impacting on pathogen colonisation. Multilocus variable-number tandem-repeat analysis (MLVA) was used to characterise all C. difficile isolates. Antibiotic susceptibility was determined by E-test and PCR-based analysis of the ermB, gyrA and gyrB genes...
October 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/26966622/a-diagnostic-algorithm-for-the-detection-of-clostridium-difficile-associated-diarrhea
#10
Özlem Yoldaş, Mustafa Altındiş, Davut Cufalı, Gülşah Aşık, Recep Keşli
BACKGROUND: Clostridium difficile is a common cause of hospital-acquired diarrhea, which is usually associated with previous antibiotic use. The clinical manifestations of C. difficile infection (CDI) may range from mild diarrhea to fulminant colitis. Clostridium difficile should be considered in diarrhea cases with a history of antibiotic use within the last 8 weeks (community-associated CDI) or with a hospital stay of at least 3 days, regardless of the duration of antibiotic use (hospital-acquired CDI)...
January 2016: Balkan Medical Journal
https://www.readbyqxmd.com/read/26912238/mapping-and-candidate-gene-screening-of-tomato-cladosporium-fulvum-resistant-gene-cf-19-based-on-high-throughput-sequencing-technology
#11
Tingting Zhao, Jingbin Jiang, Guan Liu, Shanshan He, He Zhang, Xiuling Chen, Jingfu Li, Xiangyang Xu
BACKGROUND: Tomato leaf mold is a common disease in tomato cultivation. This disease is caused by Cladosporium fulvum, which has many physiological races and differentiates rapidly. Cf genes confer resistance to C. fulvum, and the C. fulvum-tomato pathosystem is a model for the study of gene-for-gene interactions. Plants carrying the Cf-19 gene show effective resistance to C. fulvum in the field, and can be used in breeding and resistance mechanism studies as new resistant materials. In this study, we used F2 bulk specific-locus amplified fragment sequencing (SLAF-seq) and parental resequencing methods to locate and characterize the Cf-19 gene...
2016: BMC Plant Biology
https://www.readbyqxmd.com/read/26165021/-analysis-of-clostridium-difficile-associated-diarrhea-in-pediatric-patients-with-antibiotic-associated-diarrhea
#12
Guoping Cheng, Zihua Li, Xin Dai, Zaihua Wang, Ping Cai, Li Chen, Zhen Zhang
OBJECTIVE: To analyze the incidence and treatment of Clostridium difficile infection (CDI) in pediatric patients with antibiotic-associated diarrhea (AAD). METHOD: Clinical data of totally 577 pediatric patients with AAD seen from January 2012 to January 2014 were collected; those children were divided according to age into 4 groups, 0.25 -1 year, >1 -4 years, >4 -12 years and >12 -18 years old groups, and 220 healthy children were enrolled as controls...
March 2015: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/26099613/-diagnosis-of-infections-caused-by-clostridium-difficile-in-the-czech-republic-availability-possibilities-and-interpretation-of-laboratory-results
#13
M Krůtová, O Nyč
OBJECTIVE: To assess the availability of the laboratory diagnosis of infections caused by C. difficile in the Czech Republic (CR), including the range of tests used, possible combinations, and adequate interpretation of model results. MATERIAL AND METHODS: Data were collected through a web questionnaire survey with the participation of representatives of 61 public and private microbiological laboratories. The questionnaire addressed the use of diagnostic test kits and culture media in the diagnosis of C...
June 2015: Epidemiologie, Mikrobiologie, Imunologie
https://www.readbyqxmd.com/read/25753844/anti-asthma-potential-of-crocin-and-its-effect-on-mapk-signaling-pathway-in-a-murine-model-of-allergic-airway-disease
#14
Youyi Xiong, Junsong Wang, Hao Yu, Xiaolin Zhang, Chenggui Miao
CONTEXT: Crocin, a diterpenoid glucoside, has multitudinous activities such as anti-inflammation, anti-allergy, anti-oxidation and relaxing smooth muscles. OBJECTIVE: In this study, the potential of crocin as an anti-asthma agent was investigated in a murine model. MATERIALS AND METHODS: BALB/c mice were sensitized and challenged by ovalbumin (OVA) to induce allergic airway inflammation, with crocin administered one hour before every OVA challenge...
June 2015: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/25462186/comparative-performance-of-the-genexpert-c-difficile-pcr-assay-and-c-diff-quik-chek-complete-kit-assay-for-detection-of-clostridium-difficile-antigen-and-toxins-in-symptomatic-community-onset-infections
#15
COMPARATIVE STUDY
Wafaa Jamal, Eunice M Pauline, Vincent O Rotimi
OBJECTIVES: To evaluate the performance of the GeneXpert C. difficile assay and C. diff Quik Chek Complete (QCC) kit for the detection of toxins from fecal specimens and cooked meat broth (CMB) culture using toxigenic stool culture as reference method, for the diagnosis of C. difficile infection (CDI) in a community setting. METHODS: Non-repeat stool samples were tested simultaneously by GeneXpert and QCC. Toxin detection was done on neat stool samples, inoculated CMB, and isolated colonies...
December 2014: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/25122859/laboratory-detection-of-clostridium-difficile-in-piglets-in-australia
#16
Daniel R Knight, Michele M Squire, Thomas V Riley
Clostridium difficile is a well-known enteric pathogen of humans and the causative agent of high-morbidity enteritis in piglets aged 1 to 7 days. C. difficile prevalence in Australian piglets is as high as 70%. The current diagnostic assays have been validated only for human infections, and there are no published studies assessing their performance in Australian piglets. We evaluated the suitability of five assays for detecting C. difficile in 157 specimens of piglet feces. The assays included a loop-mediated isothermal amplification (LMIA)-PCR for tcdA (illumigene C...
November 2014: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/24858336/fecal-transplant-for-treatment-of-toxic-megacolon-associated-with-clostridium-difficile-colitis-in-a-patient-with-duchenne-muscular-dystrophy
#17
Shipeng Yu, Ahmed Abdelkarim, Ali Nawras, Bryan Thomas Hinch, Chimaka Mbaso, Shahul Valavoor, Fadi Safi, Jeffrey Hammersley, Jianlin Tang, Ragheb Assaly
Clostridium difficile (C diff) colitis infection is the most common cause of nosocomial infectious diarrhea and the prevalence is increasing worldwide. Toxic megacolon is a severe complication of C diff colitis associated with high mortality. Gastrointestinal (GI) comorbidity and impaired smooth muscle contraction are risk factors for the development of C diff-associated toxic megacolon. We present a case of fulminant C diff colitis with toxic megacolon in a patient with Duchenne muscular dystrophy (DMD) in the intensive care unit...
March 2016: American Journal of Therapeutics
https://www.readbyqxmd.com/read/24781004/clostridium-difficile-infection-diagnosis-in-a-paediatric-population-comparison-of-methodologies
#18
J Hart, P Putsathit, D R Knight, L Sammels, T V Riley, A Keil
The increasing incidence of Clostridium difficile infection (CDI) in paediatric hospitalised populations, combined with the emergence of hypervirulent strains, community-acquired CDI and the need for prompt treatment and infection control, makes the rapid, accurate diagnosis of CDI crucial. We validated commonly used C. difficile diagnostic tests in a paediatric hospital population. From October 2011 to January 2012, 150 consecutive stools were collected from 75 patients at a tertiary paediatric hospital in Perth, Western Australia...
September 2014: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/24579451/-experience-with-laboratory-diagnosis-of-clostridium-difficile
#19
L Bareková, E Zálabská, I Hanovcová
BACKGROUND: Clostridium difficile is currently a significant cause of nosocomial diarrhea. For several years, the number of infectious cases in the community has also been increasing. Since the beginning of 2010, quite a large increase in the number of Clostridium difficile infections (CDIs) has been noted in Pardubice Regional Hospital (PRH). The objectives of this study were to describe and evaluate the methods used in the laboratory diagnosis of CDIs in PRH, and to describe the laboratory diagnostic algorithm used here...
September 2013: Klinická Mikrobiologie a Infekc̆ní Lékar̆ství
https://www.readbyqxmd.com/read/24470904/diagnosis-of-clostridium-difficile-infection-comparison-of-four-methods-on-specimens-collected-in-cary-blair-transport-medium-and-tcdb-pcr-on-fresh-versus-frozen-samples
#20
REVIEW
Noah A Brown, William D Lebar, Carol L Young, Rosemary E Hankerd, Duane W Newton
Clostridium difficile infection (CDI) caused by toxigenic strains of C. difficile is primarily a nosocomial infection with increasing prevalence. Stool specimens are typically collected in Cary-Blair transport medium to maximize culture-based detection of common stool pathogens. The goal of this study was to establish an analytically accurate and efficient algorithm for the detection of CDI in our patient population using samples collected in Cary-Blair transport medium. In addition, we wished to determine whether the sensitivity and specificity of PCR was affected by freezing samples before testing...
March 8, 2011: Infectious Disease Reports
keyword
keyword
55942
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"