Yuezheng Lai, Yuying Zhang, Shan Zhou, Jinxu Xu, Zhanqiang Du, Ziyan Feng, Long Yu, Ziqing Zhao, Weiwei Wang, Yanting Tang, Xiuna Yang, Luke W Guddat, Fengjiang Liu, Yan Gao, Zihe Rao, Hongri Gong
Biological energy currency ATP is produced by F1 Fo -ATP synthase. However, the molecular mechanism for human ATP synthase action remains unknown. Here, we present snapshot images for three main rotational states and one substate of human ATP synthase using cryoelectron microscopy. These structures reveal that the release of ADP occurs when the β subunit of F1 Fo -ATP synthase is in the open conformation, showing how ADP binding is coordinated during synthesis. The accommodation of the symmetry mismatch between F1 and Fo motors is resolved by the torsional flexing of the entire complex, especially the γ subunit, and the rotational substep of the c subunit...
May 19, 2023: Molecular Cell